Rorug02G0539900

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0018068
Physical Location & Seq
Forward (+)
67007094 .. 67015108
8015 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0539900.1

Sequence Viewer

Length: 2007 bp
ATGGAGTCAGAGACTATGGTCTGTACCACAGATCAATGTGTTGAACAAGAGCCACAGTACACACATATTGACCAGAATGAATTCTCACAGAGAAGGCACAACAAGCAGGAGGAGGAGGAGATTCTCATATGTGAATGCAAGTATGATGAAAAGGATCTTGAAAGTGCATGTGGAGAAAGGTGCTTGAATGTGTTGACCAGCACAGAATGCACTCCTGGATATTGCCCTTGTGGTGTCTATTGTAAGAATCAGAATTCAGACATGGTTAAGGAAGAAAGGCCACAAATTACAAGGAGAGCAACCAGATCCTCCGCTTTGGCTAAAAGTGTAGTTGAAGAAACAGATACAAGCAGTCAGCACCCAAATGATGCTTCTGCGAGTTCAGACATTATTAAAGAAGAAAGCCCACAAATTACAAGGAGAACAACCCGATCCTCCGTTTTGGCCAAATGTTCAAACAATGTAGTTGAAGAAACAGACGCAAATAGTCAGCAGCAACCGACTTTAGATGACCTTGTGATTGGAGAAGAAGGAGAGTCCTTCAGCTTCGATGATCTGGTGTCTAGTTTTCCTGGTAGAGAAAGTCACATCAAAGAGCTTGTGCACCTTTTGGGTCCGCAGAACTCTCCCATGCTTCCTATATTTATATATGGAGGCTCTTCCACTGGAAAAACCAGTATTGTTCTACAAACATTTAGGCATCTTAAACGACCTTTTGTTTACTCAAGCTGCCTTACCTGTTATAATCCTCGAATCTTGTTTGAATCCATATTAAATCAATTGTTCCTTCACCGAAAGAAAGCAGAAAATGGTTATTCTAGTGCAAAGCGCTGTGAAAGGCCATCTGATTTTGTGAATTTTCTTCGCGAAGCCTTGGTTAGCATTGTTGGTAATCCTCAGGGACAAAAGTCAGGATTGAGTAGCAAAAGGTCAGGAAAGGCTAATGGAAATATGATCTACTTCATTTTTGACAATTTGGAGCGTGTCAAAGGGTGGGATAAAGGTTCTACTGTGTTACCTTTGTTGTTTAATCTCTATGACACTCTGAAAATGCCCGAGGTCGGTCTTATCTTCATCAGCAATACCTCACCGGATACATACGACTCAAATATGGGTTATGTTGAGCCTAACCCTCTATATCTTCCTGATTATACAGATGATGATCTTGGTCAAATCTTAATGAGACACCAAGCAAACAAAAAGCTTTATACCTCTTTTCTCAGTGTTGTACTAAGGCCTTTCAGTAGAATTACCAGACTAGTGGATGAATTGTCTAGTGCATTTTCAGTATTATTCAGAAAATATTGTGAACCTATTACTGATCTTGGGGTTGTTCCCAATGAAGAAATGAAGAGAAAGTTGTTTAGTCAATTTCATCCCCATATTGCTCCTGCACTGAATGAGATATTTAAGGTTTCAACTCAGCCTTCCACTGAAGTTGAAGCTAGGGAGAGAAAGCCAAAGGTTAGCACAAGGAAATCCGGAGGTTATGAAGTTGTTGACCAGTTAGATTTTCACATGTCTACCTCTGCCAAGTATCTTCTAATTTCAGCATTCCTTGCTTCAAGAAATCCAGCTACCCTTGATGCATCATTGTTCGATTCTACTGGAGGTTCTAATAATCGAAAACGAAAGAGGAAGGTCTCTGAGAAGTCAATGGAACAGAAGGAAACTGCAGAACAGGAATTACTCATGAAGGGCCCCGGAACATTCCCAATGGAGAGGTTACTAGCCATATTCCAGTGTATTACATCTGTGGCGGAAGATCCACTTGATGATGAGGAACAAGCGCAGGATGGGCTAGGGATTCAAGATGGACATTGTGGGCTCATGTCTGATGTCCTCTTACAATTGACTAGTCTCTGTGGTGCAAATTTTATTGTCAAAGGAGGAAGCTGCCCACTCGAGGGCTCAACTCGCTATCGTTCTACGGTTAGTGAAGATATGGCCCTGCAGGTAGCAAGGAGCATTAAGTTTCCTCTCTCAAAGTACTTGTACAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

668

Amino Acids

75.11

Weight (kDa)

5.66

Isoelectric Point (pI)

54.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AWS PF17907 52 - 84 9.5e-11 AWS domain
AAA_16 PF13191 190 - 332 2.5e-11 AAA ATPase domain
ORC5_lid PF21639 403 - 456 6.2e-10 ORC5, lid domain
ORC5_C PF14630 507 - 665 6.2e-39 Origin recognition complex (ORC) subunit 5 C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018068)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49510
rosa_laevigata RLG00000021862
rosa_multiflora Rmu_sc0000648.1_g000014 Rmu_sc0035653.1_g000001
rosa_roxburghii Rroxscaffold_2G00082140 Rroxscaffold_2G00082170
rosa_rugosa Rorug02G0539900 Rorug02G0539900
rosa_samantha Rh2AG611200 Rh2BG624800
rosa_wichuraiana Rw2G050720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 744
Acc36I ACCTGC 1 cut(s) 1945
AccI GTMKAC 1 cut(s) 1523
AccII CGCG 1 cut(s) 867
AccIII TCCGGA 1 cut(s) 1481
AciI CCGC 3 cut(s) 312, 617, 1760
AclWI GGATC 4 cut(s) 162, 300, 426, 1760
AcoI YGGCCR 1 cut(s) 444
AcsI RAATTY 4 cut(s) 80, 253, 856, 1873
AcuI CTGAAG 2 cut(s) 526, 1455
AfaI GTAC 5 cut(s) 25, 59, 1230, 1991, 1997
AfeI AGCGCT 1 cut(s) 830
AfiI CCNNNNNNNGG 3 cut(s) 1061, 1906, 1907
AflIII ACRYGT 1 cut(s) 1518
AhlI ACTAGT 2 cut(s) 1258, 1856
AjnI CCWGG 2 cut(s) 214, 571
AjuI GAANNNNNNNTTGG 2 cut(s) 355, 387
AluBI AGCT 7 cut(s) 546, 598, 729, 1204, 1445, 1577, 1896
AluI AGCT 7 cut(s) 546, 598, 729, 1204, 1445, 1577, 1896
Alw21I GWGCWC 1 cut(s) 606
Alw26I GTCTC 4 cut(s) 5, 1177, 1648, 1865
Alw44I GTGCAC 1 cut(s) 602
AlwI GGATC 4 cut(s) 162, 300, 426, 1760
Ama87I CYCGRG 2 cut(s) 1055, 1904
Aor13HI TCCGGA 1 cut(s) 1481
Aor51HI AGCGCT 1 cut(s) 830
AoxI GGCC 6 cut(s) 278, 444, 839, 1235, 1699, 1947
ApaI GGGCCC 1 cut(s) 1703
ApaLI GTGCAC 1 cut(s) 602
ApeKI GCWGC 3 cut(s) 493, 729, 1896
ApoI RAATTY 4 cut(s) 80, 253, 856, 1873
Asp700I GAANNNNTTC 1 cut(s) 80
AspLEI GCGC 2 cut(s) 831, 1792
AspS9I GGNCC 4 cut(s) 614, 1699, 1700, 1948
AsuC2I CCSGG 1 cut(s) 1704
AsuHPI GGTGA 2 cut(s) 782, 1080
AvaI CYCGRG 2 cut(s) 1055, 1904
AvaII GGWCC 1 cut(s) 614
AxyI CCTNAGG 1 cut(s) 897
BaeGI GKGCMC 2 cut(s) 606, 1703
BalI TGGCCA 1 cut(s) 446
BanII GRGCYC 3 cut(s) 1703, 1830, 1913
Bbv12I GWGCWC 1 cut(s) 606
BbvI GCAGC 3 cut(s) 505, 716, 1883
BccI CCATC 3 cut(s) 850, 1790, 1808
BciT130I CCWGG 2 cut(s) 216, 573
BciVI GTATCC 1 cut(s) 1087
BcnI CCSGG 1 cut(s) 1704
BcoDI GTCTC 4 cut(s) 5, 1177, 1648, 1865
BcuI ACTAGT 2 cut(s) 1258, 1856
BfaI CTAG 8 cut(s) 564, 819, 1259, 1275, 1446, 1730, 1802, 1857
BfmI CTRYAG 2 cut(s) 1674, 1952
BfoI RGCGCY 1 cut(s) 832
BfuAI ACCTGC 1 cut(s) 1945
BfuI GTATCC 1 cut(s) 1087
BisI GCNGC 3 cut(s) 494, 730, 1897
BlsI GCNGC 3 cut(s) 495, 731, 1898
BmcAI AGTACT 1 cut(s) 1991
Bme1390I CCNGG 3 cut(s) 216, 573, 1704
Bme18I GGWCC 1 cut(s) 614
BmeT110I CYCGRG 2 cut(s) 1055, 1904
BmgT120I GGNCC 4 cut(s) 614, 1699, 1700, 1948
BmiI GGNNCC 3 cut(s) 615, 1701, 1702
BmrFI CCNGG 3 cut(s) 216, 573, 1704
BmsI GCATC 4 cut(s) 358, 709, 1576, 1598
BoxI GACNNNNGTC 2 cut(s) 17, 907
BpmI CTGGAG 1 cut(s) 1629
BpuEI CTTGAG 1 cut(s) 709
BpuMI CCSGG 1 cut(s) 1704
BsaBI GATNNNNATC 1 cut(s) 1161
BsaI GGTCTC 1 cut(s) 1648
BsaJI CCNNGG 3 cut(s) 873, 1056, 1702
BsaWI WCCGGW 2 cut(s) 1090, 1481
Bsc4I CCNNNNNNNGG 3 cut(s) 1061, 1906, 1907
Bse1I ACTGG 5 cut(s) 670, 675, 1504, 1612, 1741
Bse21I CCTNAGG 1 cut(s) 897
Bse8I GATNNNNATC 1 cut(s) 1161
BseAI TCCGGA 1 cut(s) 1481
BseBI CCWGG 2 cut(s) 216, 573
BseDI CCNNGG 3 cut(s) 873, 1056, 1702
BseGI GGATG 3 cut(s) 1270, 1375, 1801
BseJI GATNNNNATC 1 cut(s) 1161
BseLI CCNNNNNNNGG 3 cut(s) 1061, 1906, 1907
BseMII CTCAG 4 cut(s) 911, 1234, 1436, 1638
BseNI ACTGG 5 cut(s) 670, 675, 1504, 1612, 1741
BseRI GAGGAG 3 cut(s) 125, 128, 131
BseSI GKGCMC 2 cut(s) 606, 1703
BseXI GCAGC 3 cut(s) 505, 716, 1883
BsgI GTGCAG 1 cut(s) 1377
Bsh1236I CGCG 1 cut(s) 867
BshFI GGCC 6 cut(s) 280, 446, 841, 1237, 1701, 1949
BsiHKAI GWGCWC 1 cut(s) 606
BsiHKCI CYCGRG 2 cut(s) 1055, 1904
BsiSI CCGG 3 cut(s) 1091, 1482, 1704
BslFI GGGAC 1 cut(s) 915
BslI CCNNNNNNNGG 3 cut(s) 1061, 1906, 1907
BsmAI GTCTC 4 cut(s) 5, 1177, 1648, 1865
BsmFI GGGAC 1 cut(s) 915
BsmI GAATGC 3 cut(s) 140, 212, 1553
BsnI GGCC 6 cut(s) 280, 446, 841, 1237, 1701, 1949
Bso31I GGTCTC 1 cut(s) 1648
BsoBI CYCGRG 2 cut(s) 1055, 1904
Bsp120I GGGCCC 1 cut(s) 1699
Bsp1286I GDGCHC 4 cut(s) 606, 1703, 1830, 1913
Bsp13I TCCGGA 1 cut(s) 1481
Bsp1407I TGTACA 1 cut(s) 1995
Bsp143I GATC 9 cut(s) 31, 154, 305, 431, 553, 954, 1162, 1321, 1765
Bsp68I TCGCGA 1 cut(s) 867
BspACI CCGC 3 cut(s) 312, 617, 1760
BspANI GGCC 6 cut(s) 280, 446, 841, 1237, 1701, 1949
BspCNI CTCAG 4 cut(s) 910, 1233, 1435, 1639
BspEI TCCGGA 1 cut(s) 1481
BspFNI CGCG 1 cut(s) 867
BspHI TCATGA 1 cut(s) 1692
BspLI GGNNCC 3 cut(s) 615, 1701, 1702
BspMAI CTGCAG 2 cut(s) 1678, 1956
BspMI ACCTGC 1 cut(s) 1945
BspPI GGATC 4 cut(s) 162, 300, 426, 1760
BspQI GCTCTTC 1 cut(s) 664
BspTNI GGTCTC 1 cut(s) 1648
BsrGI TGTACA 1 cut(s) 1995
BsrI ACTGG 5 cut(s) 670, 675, 1504, 1612, 1741
BssECI CCNNGG 3 cut(s) 873, 1056, 1702
BssMI GATC 9 cut(s) 31, 154, 305, 431, 553, 954, 1162, 1321, 1765
BssT1I CCWWGG 1 cut(s) 873
Bst2UI CCWGG 2 cut(s) 216, 573
Bst4CI ACNGT 3 cut(s) 57, 1012, 1933
Bst6I CTCTTC 2 cut(s) 664, 1346
BstAPI GCANNNNNTGC 2 cut(s) 207, 1559
BstAUI TGTACA 1 cut(s) 1995
BstDEI CTNAG 5 cut(s) 897, 1220, 1232, 1422, 1647
BstF5I GGATG 3 cut(s) 1270, 1375, 1801
BstFNI CGCG 1 cut(s) 867
BstH2I RGCGCY 1 cut(s) 832
BstHHI GCGC 2 cut(s) 831, 1792
BstKTI GATC 9 cut(s) 34, 157, 308, 434, 556, 957, 1165, 1324, 1768
BstMAI GTCTC 4 cut(s) 5, 1177, 1648, 1865
BstMBI GATC 9 cut(s) 31, 154, 305, 431, 553, 954, 1162, 1321, 1765
BstMWI GCNNNNNNNGC 5 cut(s) 103, 207, 1559, 1798, 1917
BstNI CCWGG 2 cut(s) 216, 573
BstNSI RCATGY 2 cut(s) 171, 1522
BstPAI GACNNNNGTC 2 cut(s) 17, 907
BstSCI CCNGG 3 cut(s) 214, 571, 1702
BstSFI CTRYAG 2 cut(s) 1674, 1952
BstSLI GKGCMC 2 cut(s) 606, 1703
BstUI CGCG 1 cut(s) 867
BstV1I GCAGC 3 cut(s) 505, 716, 1883
BstX2I RGATCY 3 cut(s) 154, 305, 1765
BstXI CCANNNNNNTGG 1 cut(s) 1261
BstYI RGATCY 3 cut(s) 154, 305, 1765
Bsu36I CCTNAGG 1 cut(s) 897
BsuI GTATCC 1 cut(s) 1087
BsuRI GGCC 6 cut(s) 280, 446, 841, 1237, 1701, 1949
BtsCI GGATG 3 cut(s) 1270, 1375, 1801
BtsIMutI CAGTG 5 cut(s) 663, 1228, 1394, 1431, 1748
BtuMI TCGCGA 1 cut(s) 867
BveI ACCTGC 1 cut(s) 1945
CciI TCATGA 1 cut(s) 1692
CfoI GCGC 2 cut(s) 831, 1792
Cfr13I GGNCC 4 cut(s) 614, 1699, 1700, 1948
CseI GACGC 1 cut(s) 488
Csp6I GTAC 5 cut(s) 24, 58, 1229, 1990, 1996
CspCI CAANNNNNGTGG 2 cut(s) 1420, 1455
CviAII CATG 6 cut(s) 168, 262, 631, 1519, 1693, 1831
CviQI GTAC 5 cut(s) 24, 58, 1229, 1990, 1996
DdeI CTNAG 5 cut(s) 897, 1220, 1232, 1422, 1647
DpnI GATC 9 cut(s) 33, 156, 307, 433, 555, 956, 1164, 1323, 1767
DpnII GATC 9 cut(s) 31, 154, 305, 431, 553, 954, 1162, 1321, 1765
EaeI YGGCCR 1 cut(s) 444
Eam1104I CTCTTC 2 cut(s) 664, 1346
EarI CTCTTC 2 cut(s) 664, 1346
EciI GGCGGA 1 cut(s) 1775
Eco130I CCWWGG 1 cut(s) 873
Eco147I AGGCCT 1 cut(s) 1237
Eco24I GRGCYC 3 cut(s) 1703, 1830, 1913
Eco31I GGTCTC 1 cut(s) 1648
Eco47I GGWCC 1 cut(s) 614
Eco47III AGCGCT 1 cut(s) 830
Eco57I CTGAAG 2 cut(s) 526, 1455
Eco81I CCTNAGG 1 cut(s) 897
Eco88I CYCGRG 2 cut(s) 1055, 1904
EcoO109I RGGNCCY 2 cut(s) 1699, 1700
EcoRI GAATTC 2 cut(s) 80, 253
EcoRII CCWGG 2 cut(s) 214, 571
EcoT14I CCWWGG 1 cut(s) 873
EcoT22I ATGCAT 1 cut(s) 1591
EcoT38I GRGCYC 3 cut(s) 1703, 1830, 1913
ErhI CCWWGG 1 cut(s) 873
FaeI CATG 6 cut(s) 171, 265, 634, 1522, 1696, 1834
FalI AAGNNNNNCTT 2 cut(s) 1755, 1787
FaqI GGGAC 1 cut(s) 915
FatI CATG 6 cut(s) 167, 261, 630, 1518, 1692, 1830
FauNDI CATATG 1 cut(s) 128
FblI GTMKAC 1 cut(s) 1523
Fnu4HI GCNGC 3 cut(s) 494, 730, 1897
FokI GGATG 3 cut(s) 1277, 1362, 1808
FriOI GRGCYC 3 cut(s) 1703, 1830, 1913
Fsp4HI GCNGC 3 cut(s) 494, 730, 1897
FspBI CTAG 8 cut(s) 564, 819, 1259, 1275, 1446, 1730, 1802, 1857
GlaI GCGC 2 cut(s) 830, 1791
GluI GCNGC 3 cut(s) 494, 730, 1897
GsuI CTGGAG 1 cut(s) 1629
HaeII RGCGCY 1 cut(s) 832
HaeIII GGCC 6 cut(s) 280, 446, 841, 1237, 1701, 1949
HapII CCGG 3 cut(s) 1091, 1482, 1704
HgaI GACGC 1 cut(s) 488
HhaI GCGC 2 cut(s) 831, 1792
Hin1II CATG 6 cut(s) 171, 265, 634, 1522, 1696, 1834
Hin6I GCGC 2 cut(s) 829, 1790
HinP1I GCGC 2 cut(s) 829, 1790
HincII GTYRAC 2 cut(s) 195, 1501
HindII GTYRAC 2 cut(s) 195, 1501
HindIII AAGCTT 1 cut(s) 1202
HinfI GANTC 9 cut(s) 5, 121, 247, 536, 753, 764, 1103, 1601, 1807
HpaII CCGG 3 cut(s) 1091, 1482, 1704
HphI GGTGA 2 cut(s) 782, 1080
Hpy166II GTNNAC 7 cut(s) 60, 195, 604, 721, 1310, 1501, 1524
Hpy188I TCNGA 9 cut(s) 10, 252, 259, 385, 847, 1047, 1298, 1648, 1837
Hpy188III TCNNGA 9 cut(s) 158, 866, 912, 933, 1145, 1482, 1566, 1693, 1811
Hpy8I GTNNAC 7 cut(s) 60, 195, 604, 721, 1310, 1501, 1524
HpyAV CCTTC 8 cut(s) 87, 524, 550, 797, 1437, 1633, 1660, 1690
HpyCH4III ACNGT 3 cut(s) 57, 1012, 1933
HpyF10VI GCNNNNNNNGC 5 cut(s) 103, 207, 1559, 1798, 1917
HpyF3I CTNAG 5 cut(s) 897, 1220, 1232, 1422, 1647
Hsp92II CATG 6 cut(s) 171, 265, 634, 1522, 1696, 1834
HspAI GCGC 2 cut(s) 829, 1790
Kpn2I TCCGGA 1 cut(s) 1481
Kzo9I GATC 9 cut(s) 31, 154, 305, 431, 553, 954, 1162, 1321, 1765
LguI GCTCTTC 1 cut(s) 664
LmnI GCTCC 3 cut(s) 979, 1393, 1965
Lsp1109I GCAGC 3 cut(s) 505, 716, 1883
LweI GCATC 4 cut(s) 358, 709, 1576, 1598
MaeI CTAG 8 cut(s) 564, 819, 1259, 1275, 1446, 1730, 1802, 1857
MaeIII GTNAC 3 cut(s) 584, 1014, 1725
MalI GATC 9 cut(s) 33, 156, 307, 433, 555, 956, 1164, 1323, 1767
MboI GATC 9 cut(s) 31, 154, 305, 431, 553, 954, 1162, 1321, 1765
MfeI CAATTG 2 cut(s) 779, 1850
MflI RGATCY 3 cut(s) 154, 305, 1765
MhlI GDGCHC 4 cut(s) 606, 1703, 1830, 1913
MlsI TGGCCA 1 cut(s) 446
MluNI TGGCCA 1 cut(s) 446
MlyI GAGTC 3 cut(s) 14, 545, 1097
Mox20I TGGCCA 1 cut(s) 446
Mph1103I ATGCAT 1 cut(s) 1591
MroI TCCGGA 1 cut(s) 1481
MroXI GAANNNNTTC 1 cut(s) 80
MscI TGGCCA 1 cut(s) 446
MseI TTAA 8 cut(s) 267, 393, 705, 773, 1029, 1178, 1410, 1971
MslI CAYNNNNRTG 1 cut(s) 363
Msp20I TGGCCA 1 cut(s) 446
MspI CCGG 3 cut(s) 1091, 1482, 1704
MspR9I CCNGG 3 cut(s) 216, 573, 1704
MunI CAATTG 2 cut(s) 779, 1850
Mva1269I GAATGC 3 cut(s) 140, 212, 1553
MvaI CCWGG 2 cut(s) 216, 573
MvnI CGCG 1 cut(s) 867
MwoI GCNNNNNNNGC 5 cut(s) 103, 207, 1559, 1798, 1917
NciI CCSGG 1 cut(s) 1704
NdeI CATATG 1 cut(s) 128
NdeII GATC 9 cut(s) 31, 154, 305, 431, 553, 954, 1162, 1321, 1765
NlaIII CATG 6 cut(s) 171, 265, 634, 1522, 1696, 1834
NlaIV GGNNCC 3 cut(s) 615, 1701, 1702
NmuCI GTSAC 1 cut(s) 584
NruI TCGCGA 1 cut(s) 867
NsiI ATGCAT 1 cut(s) 1591
NspI RCATGY 2 cut(s) 171, 1522
PaeR7I CTCGAG 1 cut(s) 1904
PagI TCATGA 1 cut(s) 1692
PceI AGGCCT 1 cut(s) 1237
PciI ACATGT 1 cut(s) 1518
PciSI GCTCTTC 1 cut(s) 664
PctI GAATGC 3 cut(s) 140, 212, 1553
PdmI GAANNNNTTC 1 cut(s) 80
PfeI GAWTC 6 cut(s) 121, 247, 753, 764, 1601, 1807
PfoI TCCNGGA 1 cut(s) 214
PkrI GCNGC 3 cut(s) 495, 731, 1898
PleI GAGTC 3 cut(s) 13, 544, 1097
PpsI GAGTC 3 cut(s) 13, 544, 1097
PscI ACATGT 1 cut(s) 1518
PshAI GACNNNNGTC 2 cut(s) 17, 907
PsiI TTATAA 1 cut(s) 744
Psp6I CCWGG 2 cut(s) 214, 571
PspGI CCWGG 2 cut(s) 214, 571
PspN4I GGNNCC 3 cut(s) 615, 1701, 1702
PspOMI GGGCCC 1 cut(s) 1699
PspPI GGNCC 4 cut(s) 614, 1699, 1700, 1948
PspXI VCTCGAGB 1 cut(s) 1904
PsrI GAACNNNNNNTAC 2 cut(s) 1671, 1703
PstI CTGCAG 2 cut(s) 1678, 1956
PsuI RGATCY 3 cut(s) 154, 305, 1765
RruI TCGCGA 1 cut(s) 867
RsaI GTAC 5 cut(s) 25, 59, 1230, 1991, 1997
RsaNI GTAC 5 cut(s) 24, 58, 1229, 1990, 1996
RseI CAYNNNNRTG 1 cut(s) 363
SapI GCTCTTC 1 cut(s) 664
SaqAI TTAA 8 cut(s) 267, 393, 705, 773, 1029, 1178, 1410, 1971
SatI GCNGC 3 cut(s) 494, 730, 1897
Sau3AI GATC 9 cut(s) 31, 154, 305, 431, 553, 954, 1162, 1321, 1765
Sau96I GGNCC 4 cut(s) 614, 1699, 1700, 1948
SbfI CCTGCAGG 1 cut(s) 1956
ScaI AGTACT 1 cut(s) 1991
SchI GAGTC 3 cut(s) 14, 545, 1097
ScrFI CCNGG 3 cut(s) 216, 573, 1704
SdaI CCTGCAGG 1 cut(s) 1956
SduI GDGCHC 4 cut(s) 606, 1703, 1830, 1913
SfaNI GCATC 4 cut(s) 358, 709, 1576, 1598
SfcI CTRYAG 2 cut(s) 1674, 1952
Sfr274I CTCGAG 1 cut(s) 1904
SinI GGWCC 1 cut(s) 614
SlaI CTCGAG 1 cut(s) 1904
SmiMI CAYNNNNRTG 1 cut(s) 363
SmlI CTYRAG 2 cut(s) 724, 1904
SmoI CTYRAG 2 cut(s) 724, 1904
SpeI ACTAGT 2 cut(s) 1258, 1856
Sse8387I CCTGCAGG 1 cut(s) 1956
SseBI AGGCCT 1 cut(s) 1237
SsiI CCGC 3 cut(s) 312, 617, 1760
SspI AATATT 1 cut(s) 1304
SspMI CTAG 8 cut(s) 564, 819, 1259, 1275, 1446, 1730, 1802, 1857
StuI AGGCCT 1 cut(s) 1237
StyD4I CCNGG 3 cut(s) 214, 571, 1702
StyI CCWWGG 1 cut(s) 873
TaaI ACNGT 3 cut(s) 57, 1012, 1933
TaqI TCGA 5 cut(s) 549, 751, 1599, 1624, 1905
TaqII GACCGA 1 cut(s) 1052
TatI WGTACW 4 cut(s) 57, 1228, 1989, 1995
TfiI GAWTC 6 cut(s) 121, 247, 753, 764, 1601, 1807
Tru1I TTAA 8 cut(s) 267, 393, 705, 773, 1029, 1178, 1410, 1971
Tru9I TTAA 8 cut(s) 267, 393, 705, 773, 1029, 1178, 1410, 1971
TscAI CASTG 5 cut(s) 670, 1228, 1401, 1438, 1748
TseFI GTSAC 1 cut(s) 584
TseI GCWGC 3 cut(s) 493, 729, 1896
Tsp45I GTSAC 1 cut(s) 584
TspGWI ACGGA 1 cut(s) 427
TspRI CASTG 5 cut(s) 670, 1228, 1401, 1438, 1748
VneI GTGCAC 1 cut(s) 602
VpaK11BI GGWCC 1 cut(s) 614
XapI RAATTY 4 cut(s) 80, 253, 856, 1873
XceI RCATGY 2 cut(s) 171, 1522
XhoI CTCGAG 1 cut(s) 1904
XmiI GTMKAC 1 cut(s) 1523
XmnI GAANNNNTTC 1 cut(s) 80
XspI CTAG 8 cut(s) 564, 819, 1259, 1275, 1446, 1730, 1802, 1857
ZrmI AGTACT 1 cut(s) 1991
Zsp2I ATGCAT 1 cut(s) 1591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.