Rroxscaffold_2G00082170

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0018068
Physical Location & Seq
Reverse (-)
5020879 .. 5022381
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00082170.1

Sequence Viewer

Length: 621 bp
ATGCATATGATGCGTCTGAGAAAGTTAGAAGTCATGCTTCAATCAAAGTTCATAAAAGAAATTGTAAAGCATGTTCTGAGTAGATATCAAGATGCGCACTTGAGTGTTTCCATCTTCCCCCCAAGATTAGATGCTGTCGATAATGTATATGGGACGAAAGGCATAGAGGTTATCGAGACACAGTTTCAGCAGAACCTTCAGATGATTAAAGACCTGGAGGAGCCTATCAGAAAGTCATATGTTCAGGCTCGTGATAAAGAAAGGTCCCTTATTGCTGAAATAGAAGCAATACGGAGAAAGAACGATGCTCAGGCTCAAATTATAGCAGAGCTGCAGCAAACTTCAAATTCGAGTTTCCCCTTGTACCCATTAAACTCGACAACAGATGTTATACTGGATGGAAGAAGGCCTGAATATTTTGAAGGATATGATGATGACAAAACCCTGCTCAGTTTTGTTCCTGAGAATGAGATGATGTTTGGGGATGCTAATGTTAATGATGAGTTGAAGGCAAAGATGCAATTTCTTAGTGTTGGATCTAAGGATTTCAATGAGACGGTAAGCAGTGAATCATCCAGCACAGCATTAATTCTGCAAGGGAGGAAACAGGAAAGTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

206

Amino Acids

23.67

Weight (kDa)

5.23

Isoelectric Point (pI)

54.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018068)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49510
rosa_laevigata RLG00000021862
rosa_multiflora Rmu_sc0000648.1_g000014 Rmu_sc0035653.1_g000001
rosa_roxburghii Rroxscaffold_2G00082140 Rroxscaffold_2G00082170
rosa_rugosa Rorug02G0539900 Rorug02G0539900
rosa_samantha Rh2AG611200 Rh2BG624800
rosa_wichuraiana Rw2G050720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 619
Acc16I TGCGCA 1 cut(s) 96
AclWI GGATC 1 cut(s) 544
AcsI RAATTY 1 cut(s) 346
AcuI CTGAAG 1 cut(s) 182
AfaI GTAC 1 cut(s) 365
AgsI TTSAA 5 cut(s) 41, 345, 422, 508, 550
AjnI CCWGG 1 cut(s) 213
AleI CACNNNNGTG 1 cut(s) 102
AluBI AGCT 1 cut(s) 331
AluI AGCT 1 cut(s) 331
Alw26I GTCTC 2 cut(s) 170, 548
AlwI GGATC 1 cut(s) 544
AoxI GGCC 1 cut(s) 407
ApeKI GCWGC 2 cut(s) 331, 334
ApoI RAATTY 1 cut(s) 346
AseI ATTAAT 1 cut(s) 587
AspLEI GCGC 1 cut(s) 97
AspS9I GGNCC 1 cut(s) 264
AvaII GGWCC 1 cut(s) 264
BauI CACGAG 1 cut(s) 249
BbvI GCAGC 2 cut(s) 318, 346
BccI CCATC 2 cut(s) 119, 392
BciT130I CCWGG 1 cut(s) 215
BcoDI GTCTC 2 cut(s) 170, 548
BfmI CTRYAG 1 cut(s) 332
BisI GCNGC 2 cut(s) 332, 335
BlsI GCNGC 2 cut(s) 333, 336
Bme1390I CCNGG 1 cut(s) 215
Bme18I GGWCC 1 cut(s) 264
BmgT120I GGNCC 1 cut(s) 264
BmiI GGNNCC 2 cut(s) 222, 266
BmrFI CCNGG 1 cut(s) 215
BmsI GCATC 5 cut(s) 82, 121, 295, 475, 507
BpmI CTGGAG 1 cut(s) 236
Bpu10I CCTNAGC 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 121
Bse1I ACTGG 1 cut(s) 399
BseBI CCWGG 1 cut(s) 215
BseGI GGATG 3 cut(s) 403, 490, 572
BseMII CTCAG 5 cut(s) 8, 68, 323, 453, 463
BseNI ACTGG 1 cut(s) 399
BseRI GAGGAG 1 cut(s) 233
BseXI GCAGC 2 cut(s) 318, 346
BshFI GGCC 1 cut(s) 409
BslFI GGGAC 2 cut(s) 166, 250
BsmAI GTCTC 2 cut(s) 170, 548
BsmBI CGTCTC 1 cut(s) 548
BsmFI GGGAC 2 cut(s) 166, 250
BsnI GGCC 1 cut(s) 409
Bsp143I GATC 1 cut(s) 536
BspANI GGCC 1 cut(s) 409
BspCNI CTCAG 5 cut(s) 9, 69, 322, 454, 462
BspLI GGNNCC 2 cut(s) 222, 266
BspMAI CTGCAG 1 cut(s) 336
BspPI GGATC 1 cut(s) 544
BsrI ACTGG 1 cut(s) 399
BssMI GATC 1 cut(s) 536
BssSI CACGAG 1 cut(s) 249
Bst2BI CACGAG 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 2 cut(s) 183, 559
BstAPI GCANNNNNTGC 1 cut(s) 10
BstDEI CTNAG 7 cut(s) 17, 77, 309, 449, 462, 527, 540
BstF5I GGATG 3 cut(s) 403, 490, 572
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 1 cut(s) 539
BstMAI GTCTC 2 cut(s) 170, 548
BstMBI GATC 1 cut(s) 536
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNI CCWGG 1 cut(s) 215
BstNSI RCATGY 1 cut(s) 74
BstSCI CCNGG 1 cut(s) 213
BstSFI CTRYAG 1 cut(s) 332
BstV1I GCAGC 2 cut(s) 318, 346
BstX2I RGATCY 1 cut(s) 536
BstYI RGATCY 1 cut(s) 536
BsuRI GGCC 1 cut(s) 409
BtsCI GGATG 3 cut(s) 403, 490, 572
BtsI GCAGTG 1 cut(s) 571
BtsIMutI CAGTG 1 cut(s) 571
CfoI GCGC 1 cut(s) 97
Cfr13I GGNCC 1 cut(s) 264
CseI GACGC 1 cut(s) 2
Csp6I GTAC 1 cut(s) 364
CviAII CATG 2 cut(s) 34, 71
CviJI RGCY 5 cut(s) 223, 248, 314, 331, 409
CviKI_1 RGCY 5 cut(s) 223, 248, 314, 331, 409
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 7 cut(s) 17, 77, 309, 449, 462, 527, 540
DpnI GATC 1 cut(s) 538
DpnII GATC 1 cut(s) 536
Eco147I AGGCCT 1 cut(s) 409
Eco32I GATATC 1 cut(s) 86
Eco47I GGWCC 1 cut(s) 264
Eco57I CTGAAG 1 cut(s) 182
EcoO109I RGGNCCY 1 cut(s) 264
EcoRII CCWGG 1 cut(s) 213
EcoRV GATATC 1 cut(s) 86
EcoT22I ATGCAT 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 548
FaeI CATG 2 cut(s) 37, 74
FalI AAGNNNNNCTT 2 cut(s) 21, 53
FaqI GGGAC 2 cut(s) 166, 250
FatI CATG 2 cut(s) 33, 70
FauNDI CATATG 2 cut(s) 6, 238
Fnu4HI GCNGC 2 cut(s) 332, 335
FokI GGATG 3 cut(s) 410, 497, 559
Fsp4HI GCNGC 2 cut(s) 332, 335
FspAI RTGCGCAY 1 cut(s) 96
FspI TGCGCA 1 cut(s) 96
GlaI GCGC 1 cut(s) 96
GluI GCNGC 2 cut(s) 332, 335
GsuI CTGGAG 1 cut(s) 236
HaeIII GGCC 1 cut(s) 409
HgaI GACGC 1 cut(s) 2
HhaI GCGC 1 cut(s) 97
Hin1II CATG 2 cut(s) 37, 74
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HinfI GANTC 1 cut(s) 569
Hpy188I TCNGA 4 cut(s) 18, 78, 201, 230
Hpy188III TCNNGA 4 cut(s) 89, 175, 251, 461
HpyAV CCTTC 4 cut(s) 206, 399, 416, 502
HpyCH4III ACNGT 2 cut(s) 183, 559
HpyCH4V TGCA 4 cut(s) 4, 334, 520, 595
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 7 cut(s) 17, 77, 309, 449, 462, 527, 540
Hsp92II CATG 2 cut(s) 37, 74
HspAI GCGC 1 cut(s) 95
Kzo9I GATC 1 cut(s) 536
LmnI GCTCC 1 cut(s) 220
Lsp1109I GCAGC 2 cut(s) 318, 346
LweI GCATC 5 cut(s) 82, 121, 295, 475, 507
MalI GATC 1 cut(s) 538
MboI GATC 1 cut(s) 536
MboII GAAGA 2 cut(s) 106, 414
MflI RGATCY 1 cut(s) 536
MluCI AATT 5 cut(s) 60, 318, 346, 521, 588
MmeI TCCRAC 1 cut(s) 514
MnlI CCTC 3 cut(s) 160, 211, 594
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 4 cut(s) 207, 371, 495, 587
MslI CAYNNNNRTG 1 cut(s) 102
MspR9I CCNGG 1 cut(s) 215
MvaI CCWGG 1 cut(s) 215
MwoI GCNNNNNNNGC 1 cut(s) 10
NdeI CATATG 2 cut(s) 6, 238
NdeII GATC 1 cut(s) 536
NlaIII CATG 2 cut(s) 37, 74
NlaIV GGNNCC 2 cut(s) 222, 266
NsbI TGCGCA 1 cut(s) 96
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 74
OliI CACNNNNGTG 1 cut(s) 102
PceI AGGCCT 1 cut(s) 409
PfeI GAWTC 1 cut(s) 569
PkrI GCNGC 2 cut(s) 333, 336
PpuMI RGGWCCY 1 cut(s) 264
PshBI ATTAAT 1 cut(s) 587
PsiI TTATAA 1 cut(s) 619
Psp5II RGGWCCY 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 213
PspN4I GGNNCC 2 cut(s) 222, 266
PspPI GGNCC 1 cut(s) 264
PspPPI RGGWCCY 1 cut(s) 264
PsrI GAACNNNNNNTAC 2 cut(s) 57, 89
PstI CTGCAG 1 cut(s) 336
PsuI RGATCY 1 cut(s) 536
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
RseI CAYNNNNRTG 1 cut(s) 102
SaqAI TTAA 4 cut(s) 207, 371, 495, 587
SatI GCNGC 2 cut(s) 332, 335
Sau3AI GATC 1 cut(s) 536
Sau96I GGNCC 1 cut(s) 264
ScrFI CCNGG 1 cut(s) 215
SetI ASST 5 cut(s) 171, 198, 216, 266, 333
SfaNI GCATC 5 cut(s) 82, 121, 295, 475, 507
SfcI CTRYAG 1 cut(s) 332
SinI GGWCC 1 cut(s) 264
SmiMI CAYNNNNRTG 1 cut(s) 102
SmlI CTYRAG 1 cut(s) 100
SmoI CTYRAG 1 cut(s) 100
Sse9I AATT 5 cut(s) 60, 318, 346, 521, 588
SseBI AGGCCT 1 cut(s) 409
SspI AATATT 1 cut(s) 416
StuI AGGCCT 1 cut(s) 409
StyD4I CCNGG 1 cut(s) 213
TaaI ACNGT 2 cut(s) 183, 559
TaqI TCGA 4 cut(s) 138, 174, 350, 377
TasI AATT 5 cut(s) 60, 318, 346, 521, 588
TfiI GAWTC 1 cut(s) 569
Tru1I TTAA 4 cut(s) 207, 371, 495, 587
Tru9I TTAA 4 cut(s) 207, 371, 495, 587
TscAI CASTG 1 cut(s) 571
TseI GCWGC 2 cut(s) 331, 334
TspDTI ATGAA 1 cut(s) 40
TspGWI ACGGA 1 cut(s) 307
TspRI CASTG 1 cut(s) 571
VpaK11BI GGWCC 1 cut(s) 264
VspI ATTAAT 1 cut(s) 587
XapI RAATTY 1 cut(s) 346
XceI RCATGY 1 cut(s) 74
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.