Rh2AG611200

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0018068
Physical Location & Seq
Forward (+)
83810792 .. 83816596
5805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG611200.1

Sequence Viewer

Length: 1326 bp
ATGAAGGCCAAGGTGATCGAATTCGAAGATCTGCACGAGAAGTTTAATAGAAAATTCAAGCACCTCAATCTCGACTTCGAACTCATCACTGACGAGTCCATCGGTAAGAAGACGTTCAAGATGGAGGCCTGGTTCGACACCCATAAGACCAGCTCTGCCATCTGCACCACCCTAAGCCGTTATAAGACACAAGCACTTGGTGTGCATGAAAGGAGTGGCAGGACAGAGGACAAGTTGGAGGCTTGGAGGACCTCGTTGACTGAAGCGGCTAGTCAACCTTATGGGTTTGATATGCAGGATGCCGCTACGTGGCTTCAATCGAAGTTCATTAAGGAAATTGTAAAGCATGTTTTGAGTAGATTTCAAGATGCGCACTTGAGTGTTTCCATCTTCCCACCGAAATTAGATGCTGGTAAAGAATATATGAATTCCTTGTTAAGTGTAATATCAGATGACATTCTCATTATAGGCATCTGTGGAATCGACAAAACCGCCGTGGCAAAAGATGCATATGACCAGAACTTTCATAGGTTTGAAAGCAGCAGTTTCATTAAAGATGTGAACAAGATGGAAGAACAACCCAATGGACTAGTTTCCTTGCAAAAGCGGTTCCTTTCAGATATCCTGGGAGTAGACAATGTAGAAATAAATGATGTAAAGGAAGGAACTGAGTTGATTAAAGATGTAGCAAGAAGAAAAAGAGTGCTCGTCTTTCTTGATAATGTGAATAAACTAGACCAGTTAAATGCACTTGCTAGGAGCCGGGAGTGGTTCGGCTCAGGAAGCAAAATAGTGATAACTACTAGAGATCCACAGCTGCTAAGTGTACTTGAAGTCGATAATGTATATGGGACACAAGGCGTAGAGGTTATCGAGACACGGTTTCAGCAGAACCTTCAGATGATTAAAGACCTGGAGGAGCCTATCAGAAGGTCATATGTTCAGGCTCGTGATAAAGAAAGGTCACTTATTGCTGAAATAGAAGCAATACGGAGAAAGAACAATGTTCAGGCTCAAATTATAGCAGAGCTGCAGCAAACTTCGAATTCGAGTTTCCCCTTGTACCCATTAGACTCTTCAACAGATGTTCTGCTGGATGGAAGAAGGCCTGAATATTTTGAAGGATATGATGATGACAAAACCCTGCTCAGTTTTGTTCCTGAGAATGAGATGATGATTGGGGATGCAAATGTTAATGATGAGTTGAAGGCGAAGATGCAATTTCTTAGTATTGGATCTAAGGTTTCAATGAGGATTGAGGCCGTAAGCAGTGAATCATCCAGCAAAGAATTAATTCTGCAAGGGAGGAAGCAGGAACGTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

441

Amino Acids

50.41

Weight (kDa)

5.53

Isoelectric Point (pI)

48.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 153 - 274 3.1e-07 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018068)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49510
rosa_laevigata RLG00000021862
rosa_multiflora Rmu_sc0000648.1_g000014 Rmu_sc0035653.1_g000001
rosa_roxburghii Rroxscaffold_2G00082140 Rroxscaffold_2G00082170
rosa_rugosa Rorug02G0539900 Rorug02G0539900
rosa_samantha Rh2AG611200 Rh2BG624800
rosa_wichuraiana Rw2G050720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 183, 1324
Acc16I TGCGCA 1 cut(s) 372
AccI GTMKAC 1 cut(s) 633
AciI CCGC 4 cut(s) 266, 303, 492, 607
AclI AACGTT 1 cut(s) 1318
AclWI GGATC 2 cut(s) 803, 1243
AcsI RAATTY 4 cut(s) 20, 53, 427, 1045
AcuI CTGAAG 2 cut(s) 282, 881
AdeI CACNNNGTG 1 cut(s) 200
AfaI GTAC 2 cut(s) 828, 1064
AfiI CCNNNNNNNGG 1 cut(s) 309
AhlI ACTAGT 1 cut(s) 589
AjnI CCWGG 3 cut(s) 128, 624, 912
AleI CACNNNNGTG 1 cut(s) 378
AloI GAACNNNNNNTCC 2 cut(s) 116, 148
AluBI AGCT 3 cut(s) 153, 817, 1030
AluI AGCT 3 cut(s) 153, 817, 1030
Alw21I GWGCWC 1 cut(s) 708
Alw26I GTCTC 1 cut(s) 869
AlwI GGATC 2 cut(s) 803, 1243
AoxI GGCC 4 cut(s) 6, 126, 1106, 1260
ApeKI GCWGC 4 cut(s) 540, 817, 1030, 1033
ApoI RAATTY 4 cut(s) 20, 53, 427, 1045
AseI ATTAAT 1 cut(s) 1292
Asp700I GAANNNNTTC 2 cut(s) 113, 1293
AspLEI GCGC 1 cut(s) 373
AspS9I GGNCC 1 cut(s) 249
AsuC2I CCSGG 1 cut(s) 764
AsuHPI GGTGA 1 cut(s) 25
AsuII TTCGAA 3 cut(s) 24, 78, 1043
AvaII GGWCC 1 cut(s) 249
BauI CACGAG 2 cut(s) 35, 948
BbsI GAAGAC 1 cut(s) 116
Bbv12I GWGCWC 1 cut(s) 708
BbvI GCAGC 4 cut(s) 552, 804, 1017, 1045
BccI CCATC 6 cut(s) 107, 115, 167, 395, 562, 1091
BceAI ACGGC 3 cut(s) 162, 479, 1247
BciT130I CCWGG 3 cut(s) 130, 626, 914
BcnI CCSGG 1 cut(s) 764
BcoDI GTCTC 1 cut(s) 869
BcuI ACTAGT 1 cut(s) 589
BfaI CTAG 5 cut(s) 270, 590, 734, 756, 804
BfmI CTRYAG 1 cut(s) 1031
BglII AGATCT 1 cut(s) 28
BisI GCNGC 6 cut(s) 267, 303, 541, 818, 1031, 1034
BlsI GCNGC 6 cut(s) 268, 304, 542, 819, 1032, 1035
Bme1390I CCNGG 4 cut(s) 130, 626, 764, 914
Bme18I GGWCC 1 cut(s) 249
BmgT120I GGNCC 1 cut(s) 249
BmiI GGNNCC 3 cut(s) 611, 761, 921
BmrFI CCNGG 4 cut(s) 130, 626, 764, 914
BmsI GCATC 7 cut(s) 289, 358, 397, 480, 496, 1174, 1206
BpiI GAAGAC 1 cut(s) 116
BpmI CTGGAG 1 cut(s) 935
Bpu10I CCTNAGC 2 cut(s) 173, 778
Bpu14I TTCGAA 3 cut(s) 24, 78, 1043
BpuEI CTTGAG 1 cut(s) 397
BpuMI CCSGG 1 cut(s) 764
BsaAI YACGTR 1 cut(s) 309
BsaJI CCNNGG 3 cut(s) 9, 495, 625
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 1 cut(s) 309
Bse1I ACTGG 1 cut(s) 739
BseBI CCWGG 3 cut(s) 130, 626, 914
BseDI CCNNGG 3 cut(s) 9, 495, 625
BseGI GGATG 4 cut(s) 304, 1102, 1189, 1277
BseLI CCNNNNNNNGG 1 cut(s) 309
BseMII CTCAG 4 cut(s) 660, 792, 1152, 1162
BseNI ACTGG 1 cut(s) 739
BseRI GAGGAG 1 cut(s) 932
BseXI GCAGC 4 cut(s) 552, 804, 1017, 1045
BsgI GTGCAG 2 cut(s) 17, 148
BshFI GGCC 4 cut(s) 8, 128, 1108, 1262
BsiHKAI GWGCWC 1 cut(s) 708
BsiSI CCGG 1 cut(s) 763
BslFI GGGAC 1 cut(s) 865
BslI CCNNNNNNNGG 1 cut(s) 309
BsmAI GTCTC 1 cut(s) 869
BsmFI GGGAC 1 cut(s) 865
BsnI GGCC 4 cut(s) 8, 128, 1108, 1262
Bsp119I TTCGAA 3 cut(s) 24, 78, 1043
Bsp1286I GDGCHC 1 cut(s) 708
Bsp143I GATC 4 cut(s) 15, 28, 808, 1235
BspACI CCGC 4 cut(s) 266, 303, 492, 607
BspANI GGCC 4 cut(s) 8, 128, 1108, 1262
BspCNI CTCAG 4 cut(s) 661, 791, 1153, 1161
BspLI GGNNCC 3 cut(s) 611, 761, 921
BspMAI CTGCAG 1 cut(s) 1035
BspPI GGATC 2 cut(s) 803, 1243
BspT104I TTCGAA 3 cut(s) 24, 78, 1043
BsrI ACTGG 1 cut(s) 739
BssECI CCNNGG 3 cut(s) 9, 495, 625
BssMI GATC 4 cut(s) 15, 28, 808, 1235
BssSI CACGAG 2 cut(s) 35, 948
BssT1I CCWWGG 1 cut(s) 9
Bst2BI CACGAG 2 cut(s) 35, 948
Bst2UI CCWGG 3 cut(s) 130, 626, 914
Bst4CI ACNGT 1 cut(s) 882
Bst6I CTCTTC 1 cut(s) 1081
BstAPI GCANNNNNTGC 1 cut(s) 506
BstBAI YACGTR 1 cut(s) 309
BstBI TTCGAA 3 cut(s) 24, 78, 1043
BstDEI CTNAG 8 cut(s) 173, 669, 778, 821, 1148, 1161, 1226, 1239
BstDSI CCRYGG 1 cut(s) 495
BstF5I GGATG 4 cut(s) 304, 1102, 1189, 1277
BstHHI GCGC 1 cut(s) 373
BstKTI GATC 4 cut(s) 18, 31, 811, 1238
BstMAI GTCTC 1 cut(s) 869
BstMBI GATC 4 cut(s) 15, 28, 808, 1235
BstMWI GCNNNNNNNGC 2 cut(s) 506, 783
BstNI CCWGG 3 cut(s) 130, 626, 914
BstNSI RCATGY 1 cut(s) 350
BstSCI CCNGG 4 cut(s) 128, 624, 762, 912
BstSFI CTRYAG 1 cut(s) 1031
BstV1I GCAGC 4 cut(s) 552, 804, 1017, 1045
BstV2I GAAGAC 1 cut(s) 116
BstX2I RGATCY 3 cut(s) 28, 808, 1235
BstYI RGATCY 3 cut(s) 28, 808, 1235
BsuRI GGCC 4 cut(s) 8, 128, 1108, 1262
BtgI CCRYGG 1 cut(s) 495
BtsCI GGATG 4 cut(s) 304, 1102, 1189, 1277
BtsI GCAGTG 1 cut(s) 1276
BtsIMutI CAGTG 2 cut(s) 87, 1276
CfoI GCGC 1 cut(s) 373
Cfr13I GGNCC 1 cut(s) 249
Csp6I GTAC 2 cut(s) 827, 1063
CviAII CATG 2 cut(s) 206, 347
CviQI GTAC 2 cut(s) 827, 1063
DdeI CTNAG 8 cut(s) 173, 669, 778, 821, 1148, 1161, 1226, 1239
DpnI GATC 4 cut(s) 17, 30, 810, 1237
DpnII GATC 4 cut(s) 15, 28, 808, 1235
DraIII CACNNNGTG 1 cut(s) 200
Eam1104I CTCTTC 1 cut(s) 1081
EarI CTCTTC 1 cut(s) 1081
Eco130I CCWWGG 1 cut(s) 9
Eco147I AGGCCT 2 cut(s) 128, 1108
Eco32I GATATC 1 cut(s) 622
Eco47I GGWCC 1 cut(s) 249
Eco57I CTGAAG 2 cut(s) 282, 881
EcoO109I RGGNCCY 1 cut(s) 249
EcoRI GAATTC 3 cut(s) 20, 427, 1045
EcoRII CCWGG 3 cut(s) 128, 624, 912
EcoRV GATATC 1 cut(s) 622
EcoT14I CCWWGG 1 cut(s) 9
EcoT22I ATGCAT 1 cut(s) 511
ErhI CCWWGG 1 cut(s) 9
FaeI CATG 2 cut(s) 209, 350
FaqI GGGAC 1 cut(s) 865
FatI CATG 2 cut(s) 205, 346
FauNDI CATATG 2 cut(s) 511, 937
FblI GTMKAC 1 cut(s) 633
Fnu4HI GCNGC 6 cut(s) 267, 303, 541, 818, 1031, 1034
FokI GGATG 4 cut(s) 311, 1109, 1196, 1264
Fsp4HI GCNGC 6 cut(s) 267, 303, 541, 818, 1031, 1034
FspAI RTGCGCAY 1 cut(s) 372
FspBI CTAG 5 cut(s) 270, 590, 734, 756, 804
FspI TGCGCA 1 cut(s) 372
GlaI GCGC 1 cut(s) 372
GluI GCNGC 6 cut(s) 267, 303, 541, 818, 1031, 1034
GsuI CTGGAG 1 cut(s) 935
HaeIII GGCC 4 cut(s) 8, 128, 1108, 1262
HapII CCGG 1 cut(s) 763
HhaI GCGC 1 cut(s) 373
Hin1II CATG 2 cut(s) 209, 350
Hin6I GCGC 1 cut(s) 371
HinP1I GCGC 1 cut(s) 371
HincII GTYRAC 2 cut(s) 258, 275
HindII GTYRAC 2 cut(s) 258, 275
HinfI GANTC 4 cut(s) 95, 480, 1073, 1274
HpaII CCGG 1 cut(s) 763
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 5 cut(s) 258, 275, 562, 634, 827
Hpy188I TCNGA 4 cut(s) 451, 619, 900, 929
Hpy188III TCNNGA 8 cut(s) 71, 118, 365, 716, 780, 874, 950, 1160
Hpy8I GTNNAC 5 cut(s) 258, 275, 562, 634, 827
HpyAV CCTTC 6 cut(s) 656, 905, 924, 1098, 1115, 1201
HpyCH4III ACNGT 1 cut(s) 882
HpyCH4IV ACGT 3 cut(s) 113, 308, 1318
HpyF10VI GCNNNNNNNGC 2 cut(s) 506, 783
HpyF3I CTNAG 8 cut(s) 173, 669, 778, 821, 1148, 1161, 1226, 1239
HpySE526I ACGT 3 cut(s) 113, 308, 1318
Hsp92II CATG 2 cut(s) 209, 350
HspAI GCGC 1 cut(s) 371
Kzo9I GATC 4 cut(s) 15, 28, 808, 1235
LmnI GCTCC 2 cut(s) 759, 919
Lsp1109I GCAGC 4 cut(s) 552, 804, 1017, 1045
LweI GCATC 7 cut(s) 289, 358, 397, 480, 496, 1174, 1206
MaeI CTAG 5 cut(s) 270, 590, 734, 756, 804
MaeII ACGT 3 cut(s) 113, 308, 1318
MaeIII GTNAC 1 cut(s) 963
MalI GATC 4 cut(s) 17, 30, 810, 1237
MboI GATC 4 cut(s) 15, 28, 808, 1235
MboII GAAGA 8 cut(s) 38, 121, 382, 584, 705, 1068, 1113, 1225
MflI RGATCY 3 cut(s) 28, 808, 1235
MhlI GDGCHC 1 cut(s) 708
MlyI GAGTC 2 cut(s) 104, 1067
MmeI TCCRAC 1 cut(s) 216
Mph1103I ATGCAT 1 cut(s) 511
MroXI GAANNNNTTC 2 cut(s) 113, 1293
MseI TTAA 9 cut(s) 45, 330, 437, 552, 678, 743, 906, 1194, 1292
MslI CAYNNNNRTG 1 cut(s) 378
MspA1I CMGCKG 1 cut(s) 817
MspI CCGG 1 cut(s) 763
MspR9I CCNGG 4 cut(s) 130, 626, 764, 914
MvaI CCWGG 3 cut(s) 130, 626, 914
MwoI GCNNNNNNNGC 2 cut(s) 506, 783
NciI CCSGG 1 cut(s) 764
NdeI CATATG 2 cut(s) 511, 937
NdeII GATC 4 cut(s) 15, 28, 808, 1235
NlaIII CATG 2 cut(s) 209, 350
NlaIV GGNNCC 3 cut(s) 611, 761, 921
NmuCI GTSAC 1 cut(s) 963
NsbI TGCGCA 1 cut(s) 372
NsiI ATGCAT 1 cut(s) 511
NspI RCATGY 1 cut(s) 350
NspV TTCGAA 3 cut(s) 24, 78, 1043
OliI CACNNNNGTG 1 cut(s) 378
PceI AGGCCT 2 cut(s) 128, 1108
PdmI GAANNNNTTC 2 cut(s) 113, 1293
PfeI GAWTC 2 cut(s) 480, 1274
PkrI GCNGC 6 cut(s) 268, 304, 542, 819, 1032, 1035
PleI GAGTC 2 cut(s) 103, 1067
PpsI GAGTC 2 cut(s) 103, 1067
Ppu21I YACGTR 1 cut(s) 309
PpuMI RGGWCCY 1 cut(s) 249
PshBI ATTAAT 1 cut(s) 1292
PsiI TTATAA 2 cut(s) 183, 1324
Psp1406I AACGTT 1 cut(s) 1318
Psp5II RGGWCCY 1 cut(s) 249
Psp6I CCWGG 3 cut(s) 128, 624, 912
PspGI CCWGG 3 cut(s) 128, 624, 912
PspN4I GGNNCC 3 cut(s) 611, 761, 921
PspPI GGNCC 1 cut(s) 249
PspPPI RGGWCCY 1 cut(s) 249
PstI CTGCAG 1 cut(s) 1035
PsuI RGATCY 3 cut(s) 28, 808, 1235
PvuII CAGCTG 1 cut(s) 817
RsaI GTAC 2 cut(s) 828, 1064
RsaNI GTAC 2 cut(s) 827, 1063
RseI CAYNNNNRTG 1 cut(s) 378
SaqAI TTAA 9 cut(s) 45, 330, 437, 552, 678, 743, 906, 1194, 1292
SatI GCNGC 6 cut(s) 267, 303, 541, 818, 1031, 1034
Sau3AI GATC 4 cut(s) 15, 28, 808, 1235
Sau96I GGNCC 1 cut(s) 249
SchI GAGTC 2 cut(s) 104, 1067
ScrFI CCNGG 4 cut(s) 130, 626, 764, 914
SduI GDGCHC 1 cut(s) 708
SfaNI GCATC 7 cut(s) 289, 358, 397, 480, 496, 1174, 1206
SfcI CTRYAG 1 cut(s) 1031
SfuI TTCGAA 3 cut(s) 24, 78, 1043
SinI GGWCC 1 cut(s) 249
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 1 cut(s) 376
SmoI CTYRAG 1 cut(s) 376
SpeI ACTAGT 1 cut(s) 589
SseBI AGGCCT 2 cut(s) 128, 1108
SsiI CCGC 4 cut(s) 266, 303, 492, 607
SspI AATATT 1 cut(s) 1115
SspMI CTAG 5 cut(s) 270, 590, 734, 756, 804
StuI AGGCCT 2 cut(s) 128, 1108
StyD4I CCNGG 4 cut(s) 128, 624, 762, 912
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 1 cut(s) 882
TaiI ACGT 3 cut(s) 116, 311, 1321
TatI WGTACW 1 cut(s) 826
TauI GCSGC 2 cut(s) 269, 305
TfiI GAWTC 2 cut(s) 480, 1274
Tru1I TTAA 9 cut(s) 45, 330, 437, 552, 678, 743, 906, 1194, 1292
Tru9I TTAA 9 cut(s) 45, 330, 437, 552, 678, 743, 906, 1194, 1292
TscAI CASTG 2 cut(s) 94, 1276
TseFI GTSAC 1 cut(s) 963
TseI GCWGC 4 cut(s) 540, 817, 1030, 1033
Tsp45I GTSAC 1 cut(s) 963
TspDTI ATGAA 6 cut(s) 17, 222, 316, 440, 515, 538
TspGWI ACGGA 1 cut(s) 1006
TspRI CASTG 2 cut(s) 94, 1276
VpaK11BI GGWCC 1 cut(s) 249
VspI ATTAAT 1 cut(s) 1292
XapI RAATTY 4 cut(s) 20, 53, 427, 1045
XceI RCATGY 1 cut(s) 350
XmiI GTMKAC 1 cut(s) 633
XmnI GAANNNNTTC 2 cut(s) 113, 1293
XspI CTAG 5 cut(s) 270, 590, 734, 756, 804
Zsp2I ATGCAT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.