Rroxscaffold_2G00082140

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0018068
Physical Location & Seq
Reverse (-)
5007537 .. 5009957
2421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00082140.1

Sequence Viewer

Length: 966 bp
ATGACTAGGACAAAACCGCTCATGGCATCGTCGTTTTCCTTCTATTCGTCAGCAACAAGGCGCAGGCACGACGTGTTCTTGAGCTTCCGAGGCGAAGACACCCGCCACAACATAACCAAACACCTCCACAGCGCTTTAGTCGAAGCAGGCGTTCCACCATTTAAAGACGATGTCAATCTTCAAAATGGCAGCGAGATCGCATCCGATCTGCTCAAGGCCATGCAGATGTCGAGGATCGCCATCATCGTGTTTTCGAACAACTACGCGAGCTCCAGATGGTGTCTGGACGAACTGGTCATGATTGTAGAGTGTAACAGGGAAGCCGGTCAGATCATTTTGCCCATTTTCTGCGATGTAAATCCCTGTGATGTGCAGCAGCATAAGGGGAAGTTTTCAGAAGGCATCTGTGGAATTGACAAAACTGCCATGGCAAAAGATGCATATGACCAGAATTTTCATAGGTTTGAAAGCAGCAGTTTCATTAAAGATGTGCACAAGATGGCAGAACAACCCAATGGACTAGTTTCCTTGCAAAAGCGGTTCCTTTCAGATATCCTGGGAGTAGACAGTGTAGGAATAAATGATATTAAGGAAGGAACTGAGTTGATTAAAGATGTAGGAAGAAGAAAAAGAGTGCTCGTCTTTCTTGATAATGTGAATAAACTGGACCAGTTAAATGCACTTGCTAGGAGCCGGGAGTGGTTCGGCTCAGGAAGCAGAATGGTGATAACTACTAGAGATCCACAACTGCTAAGTGTACTTGAAGTCGACAATTGCGCAAATTATAGCAGAGGTGCAACAATATTCGAATTCCAGTTTCCCCTAGCCTTGTATGCATTAGACTCGGCAACAGATGTTCTACTGGATGGAAGAGGGCATGAAAAATTTTATGGATATGATGATTACAAAACCCTGCTCAATTTGGTTCCTGAGAATGAGATGATAATTGGAGCTGCTAAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

321

Amino Acids

36.17

Weight (kDa)

6.09

Isoelectric Point (pI)

47.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 138 1.9e-35 TIR domain
TIR_2 PF13676 25 - 141 4.6e-15 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018068)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49510
rosa_laevigata RLG00000021862
rosa_multiflora Rmu_sc0000648.1_g000014 Rmu_sc0035653.1_g000001
rosa_roxburghii Rroxscaffold_2G00082140 Rroxscaffold_2G00082170
rosa_rugosa Rorug02G0539900 Rorug02G0539900
rosa_samantha Rh2AG611200 Rh2BG624800
rosa_wichuraiana Rw2G050720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 293
Acc16I TGCGCA 1 cut(s) 778
AccBSI CCGCTC 1 cut(s) 19
AccI GTMKAC 2 cut(s) 564, 768
AccII CGCG 1 cut(s) 266
AciI CCGC 3 cut(s) 17, 103, 538
AclWI GGATC 2 cut(s) 242, 734
AcsI RAATTY 3 cut(s) 451, 809, 884
AdeI CACNNNGTG 1 cut(s) 73
AfaI GTAC 1 cut(s) 759
AfeI AGCGCT 1 cut(s) 133
AflIII ACRYGT 1 cut(s) 72
AgsI TTSAA 3 cut(s) 182, 467, 764
AhlI ACTAGT 1 cut(s) 520
AjiI CACGTC 1 cut(s) 73
AjnI CCWGG 1 cut(s) 555
AluBI AGCT 3 cut(s) 84, 270, 953
AluI AGCT 3 cut(s) 84, 270, 953
Alw21I GWGCWC 3 cut(s) 272, 495, 639
Alw44I GTGCAC 1 cut(s) 491
AlwI GGATC 2 cut(s) 242, 734
Aor51HI AGCGCT 1 cut(s) 133
AoxI GGCC 1 cut(s) 216
ApaLI GTGCAC 1 cut(s) 491
ApeKI GCWGC 5 cut(s) 189, 373, 376, 471, 953
ApoI RAATTY 3 cut(s) 451, 809, 884
AspLEI GCGC 3 cut(s) 63, 134, 779
AspS9I GGNCC 1 cut(s) 667
AsuC2I CCSGG 1 cut(s) 695
AsuHPI GGTGA 1 cut(s) 736
AsuII TTCGAA 2 cut(s) 254, 807
AvaII GGWCC 1 cut(s) 667
BaeGI GKGCMC 1 cut(s) 495
BanII GRGCYC 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 102
Bbv12I GWGCWC 3 cut(s) 272, 495, 639
BbvI GCAGC 5 cut(s) 201, 385, 388, 483, 940
BccI CCATC 4 cut(s) 248, 270, 493, 860
BcgI CGANNNNNNTGC 4 cut(s) 178, 212, 825, 859
BciT130I CCWGG 1 cut(s) 557
BcnI CCSGG 1 cut(s) 695
BcuI ACTAGT 1 cut(s) 520
BfaI CTAG 5 cut(s) 6, 521, 687, 735, 824
BfoI RGCGCY 1 cut(s) 135
BisI GCNGC 5 cut(s) 190, 374, 377, 472, 954
BlpI GCTNAGC 1 cut(s) 957
BlsI GCNGC 5 cut(s) 191, 375, 378, 473, 955
Bme1390I CCNGG 2 cut(s) 557, 695
Bme18I GGWCC 1 cut(s) 667
BmgBI CACGTC 1 cut(s) 73
BmgT120I GGNCC 1 cut(s) 667
BmiI GGNNCC 3 cut(s) 542, 692, 927
BmrFI CCNGG 2 cut(s) 557, 695
BmsI GCATC 4 cut(s) 35, 209, 411, 427
BpiI GAAGAC 1 cut(s) 102
BpmI CTGGAG 1 cut(s) 256
Bpu10I CCTNAGC 1 cut(s) 709
Bpu1102I GCTNAGC 1 cut(s) 957
Bpu14I TTCGAA 2 cut(s) 254, 807
BpuEI CTTGAG 2 cut(s) 100, 197
BpuMI CCSGG 1 cut(s) 695
BsaBI GATNNNNATC 3 cut(s) 174, 239, 357
BsaJI CCNNGG 3 cut(s) 88, 426, 556
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 5 cut(s) 297, 669, 670, 814, 867
Bse8I GATNNNNATC 3 cut(s) 174, 239, 357
BseBI CCWGG 1 cut(s) 557
BseDI CCNNGG 3 cut(s) 88, 426, 556
BseGI GGATG 2 cut(s) 200, 871
BseJI GATNNNNATC 3 cut(s) 174, 239, 357
BseMII CTCAG 3 cut(s) 591, 723, 921
BseNI ACTGG 5 cut(s) 297, 669, 670, 814, 867
BseSI GKGCMC 1 cut(s) 495
BseXI GCAGC 5 cut(s) 201, 385, 388, 483, 940
BsgI GTGCAG 1 cut(s) 392
Bsh1236I CGCG 1 cut(s) 266
BshFI GGCC 1 cut(s) 218
BsiHKAI GWGCWC 3 cut(s) 272, 495, 639
BsiSI CCGG 2 cut(s) 324, 694
BsnI GGCC 1 cut(s) 218
Bsp119I TTCGAA 2 cut(s) 254, 807
Bsp1286I GDGCHC 3 cut(s) 272, 495, 639
Bsp143I GATC 5 cut(s) 195, 205, 234, 330, 739
Bsp1720I GCTNAGC 1 cut(s) 957
Bsp19I CCATGG 1 cut(s) 426
BspACI CCGC 3 cut(s) 17, 103, 538
BspANI GGCC 1 cut(s) 218
BspCNI CTCAG 3 cut(s) 592, 722, 922
BspFNI CGCG 1 cut(s) 266
BspHI TCATGA 1 cut(s) 297
BspLI GGNNCC 3 cut(s) 542, 692, 927
BspPI GGATC 2 cut(s) 242, 734
BspT104I TTCGAA 2 cut(s) 254, 807
BsrBI CCGCTC 1 cut(s) 19
BsrFI RCCGGY 1 cut(s) 323
BsrI ACTGG 5 cut(s) 297, 669, 670, 814, 867
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 3 cut(s) 88, 426, 556
BssMI GATC 5 cut(s) 195, 205, 234, 330, 739
BssT1I CCWWGG 1 cut(s) 426
Bst2UI CCWGG 1 cut(s) 557
Bst4CI ACNGT 1 cut(s) 569
Bst6I CTCTTC 1 cut(s) 865
BstAPI GCANNNNNTGC 1 cut(s) 437
BstBI TTCGAA 2 cut(s) 254, 807
BstC8I GCNNGC 3 cut(s) 65, 148, 268
BstDEI CTNAG 5 cut(s) 600, 709, 752, 930, 957
BstDSI CCRYGG 1 cut(s) 426
BstF5I GGATG 2 cut(s) 200, 871
BstFNI CGCG 1 cut(s) 266
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 3 cut(s) 63, 134, 779
BstKTI GATC 5 cut(s) 198, 208, 237, 333, 742
BstMBI GATC 5 cut(s) 195, 205, 234, 330, 739
BstMWI GCNNNNNNNGC 4 cut(s) 90, 437, 714, 833
BstNI CCWGG 1 cut(s) 557
BstSCI CCNGG 2 cut(s) 555, 693
BstSLI GKGCMC 1 cut(s) 495
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 5 cut(s) 201, 385, 388, 483, 940
BstV2I GAAGAC 1 cut(s) 102
BstX2I RGATCY 1 cut(s) 739
BstYI RGATCY 1 cut(s) 739
BsuRI GGCC 1 cut(s) 218
BtgI CCRYGG 1 cut(s) 426
BtgZI GCGATG 1 cut(s) 366
BtrI CACGTC 1 cut(s) 73
BtsCI GGATG 2 cut(s) 200, 871
BtsIMutI CAGTG 1 cut(s) 574
Cac8I GCNNGC 3 cut(s) 65, 148, 268
CciI TCATGA 1 cut(s) 297
CfoI GCGC 3 cut(s) 63, 134, 779
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 1 cut(s) 667
Csp6I GTAC 1 cut(s) 758
CviAII CATG 5 cut(s) 22, 220, 298, 427, 878
CviJI RGCY 8 cut(s) 84, 218, 270, 323, 693, 708, 827, 953
CviKI_1 RGCY 8 cut(s) 84, 218, 270, 323, 693, 708, 827, 953
CviQI GTAC 1 cut(s) 758
DdeI CTNAG 5 cut(s) 600, 709, 752, 930, 957
DpnI GATC 5 cut(s) 197, 207, 236, 332, 741
DpnII GATC 5 cut(s) 195, 205, 234, 330, 739
DraI TTTAAA 1 cut(s) 163
DraIII CACNNNGTG 1 cut(s) 73
DrdI GACNNNNNNGTC 1 cut(s) 293
DseDI GACNNNNNNGTC 1 cut(s) 293
Eam1104I CTCTTC 1 cut(s) 865
EarI CTCTTC 1 cut(s) 865
Ecl136II GAGCTC 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 426
Eco24I GRGCYC 1 cut(s) 272
Eco32I GATATC 1 cut(s) 553
Eco47I GGWCC 1 cut(s) 667
Eco47III AGCGCT 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 270
EcoICRI GAGCTC 1 cut(s) 270
EcoRI GAATTC 1 cut(s) 809
EcoRII CCWGG 1 cut(s) 555
EcoRV GATATC 1 cut(s) 553
EcoT14I CCWWGG 1 cut(s) 426
EcoT22I ATGCAT 2 cut(s) 442, 838
EcoT38I GRGCYC 1 cut(s) 272
ErhI CCWWGG 1 cut(s) 426
FaeI CATG 5 cut(s) 25, 223, 301, 430, 881
FatI CATG 5 cut(s) 21, 219, 297, 426, 877
FauI CCCGC 1 cut(s) 110
FauNDI CATATG 1 cut(s) 442
FblI GTMKAC 2 cut(s) 564, 768
Fnu4HI GCNGC 5 cut(s) 190, 374, 377, 472, 954
FokI GGATG 2 cut(s) 187, 878
FriOI GRGCYC 1 cut(s) 272
Fsp4HI GCNGC 5 cut(s) 190, 374, 377, 472, 954
FspBI CTAG 5 cut(s) 6, 521, 687, 735, 824
FspI TGCGCA 1 cut(s) 778
GlaI GCGC 3 cut(s) 62, 133, 778
GluI GCNGC 5 cut(s) 190, 374, 377, 472, 954
GsuI CTGGAG 1 cut(s) 256
HaeII RGCGCY 1 cut(s) 135
HaeIII GGCC 1 cut(s) 218
HapII CCGG 2 cut(s) 324, 694
HhaI GCGC 3 cut(s) 63, 134, 779
Hin1II CATG 5 cut(s) 25, 223, 301, 430, 881
Hin6I GCGC 3 cut(s) 61, 132, 777
HinP1I GCGC 3 cut(s) 61, 132, 777
HincII GTYRAC 1 cut(s) 769
HindII GTYRAC 1 cut(s) 769
HinfI GANTC 1 cut(s) 842
HpaII CCGG 2 cut(s) 324, 694
HphI GGTGA 1 cut(s) 736
Hpy166II GTNNAC 4 cut(s) 493, 565, 758, 769
Hpy188I TCNGA 5 cut(s) 89, 205, 330, 397, 550
Hpy188III TCNNGA 7 cut(s) 79, 273, 284, 298, 647, 711, 929
Hpy8I GTNNAC 4 cut(s) 493, 565, 758, 769
Hpy99I CGWCG 2 cut(s) 34, 74
HpyAV CCTTC 3 cut(s) 49, 392, 587
HpyCH4III ACNGT 1 cut(s) 569
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 8 cut(s) 223, 373, 440, 493, 532, 680, 797, 836
HpyF10VI GCNNNNNNNGC 4 cut(s) 90, 437, 714, 833
HpyF3I CTNAG 5 cut(s) 600, 709, 752, 930, 957
HpySE526I ACGT 1 cut(s) 72
Hsp92II CATG 5 cut(s) 25, 223, 301, 430, 881
HspAI GCGC 3 cut(s) 61, 132, 777
Kzo9I GATC 5 cut(s) 195, 205, 234, 330, 739
LmnI GCTCC 3 cut(s) 275, 690, 950
Lsp1109I GCAGC 5 cut(s) 201, 385, 388, 483, 940
LweI GCATC 4 cut(s) 35, 209, 411, 427
MaeI CTAG 5 cut(s) 6, 521, 687, 735, 824
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 1 cut(s) 311
MalI GATC 5 cut(s) 197, 207, 236, 332, 741
MbiI CCGCTC 1 cut(s) 19
MboI GATC 5 cut(s) 195, 205, 234, 330, 739
MboII GAAGA 5 cut(s) 107, 170, 633, 636, 882
MfeI CAATTG 1 cut(s) 772
MflI RGATCY 1 cut(s) 739
MhlI GDGCHC 3 cut(s) 272, 495, 639
MluCI AATT 8 cut(s) 411, 451, 772, 781, 809, 884, 919, 945
MlyI GAGTC 1 cut(s) 836
MnlI CCTC 5 cut(s) 83, 134, 225, 785, 866
Mph1103I ATGCAT 2 cut(s) 442, 838
MseI TTAA 5 cut(s) 162, 483, 588, 609, 674
MslI CAYNNNNRTG 2 cut(s) 224, 245
MspI CCGG 2 cut(s) 324, 694
MspR9I CCNGG 2 cut(s) 557, 695
MunI CAATTG 1 cut(s) 772
MvaI CCWGG 1 cut(s) 557
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 4 cut(s) 90, 437, 714, 833
NciI CCSGG 1 cut(s) 695
NcoI CCATGG 1 cut(s) 426
NdeI CATATG 1 cut(s) 442
NdeII GATC 5 cut(s) 195, 205, 234, 330, 739
NlaIII CATG 5 cut(s) 25, 223, 301, 430, 881
NlaIV GGNNCC 3 cut(s) 542, 692, 927
NmeAIII GCCGAG 1 cut(s) 824
NsbI TGCGCA 1 cut(s) 778
NsiI ATGCAT 2 cut(s) 442, 838
NspV TTCGAA 2 cut(s) 254, 807
PagI TCATGA 1 cut(s) 297
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 243
PflFI GACNNNGTC 1 cut(s) 170
PkrI GCNGC 5 cut(s) 191, 375, 378, 473, 955
PleI GAGTC 1 cut(s) 836
PpsI GAGTC 1 cut(s) 836
Psp124BI GAGCTC 1 cut(s) 272
Psp6I CCWGG 1 cut(s) 555
PspGI CCWGG 1 cut(s) 555
PspN4I GGNNCC 3 cut(s) 542, 692, 927
PspPI GGNCC 1 cut(s) 667
PsuI RGATCY 1 cut(s) 739
PsyI GACNNNGTC 1 cut(s) 170
RsaI GTAC 1 cut(s) 759
RsaNI GTAC 1 cut(s) 758
RseI CAYNNNNRTG 2 cut(s) 224, 245
SacI GAGCTC 1 cut(s) 272
SalI GTCGAC 1 cut(s) 767
SaqAI TTAA 5 cut(s) 162, 483, 588, 609, 674
SatI GCNGC 5 cut(s) 190, 374, 377, 472, 954
Sau3AI GATC 5 cut(s) 195, 205, 234, 330, 739
Sau96I GGNCC 1 cut(s) 667
SchI GAGTC 1 cut(s) 836
ScrFI CCNGG 2 cut(s) 557, 695
SduI GDGCHC 3 cut(s) 272, 495, 639
SetI ASST 7 cut(s) 75, 86, 126, 272, 464, 796, 955
SfaNI GCATC 4 cut(s) 35, 209, 411, 427
SfuI TTCGAA 2 cut(s) 254, 807
SinI GGWCC 1 cut(s) 667
SmiMI CAYNNNNRTG 2 cut(s) 224, 245
SmlI CTYRAG 2 cut(s) 79, 212
SmoI CTYRAG 2 cut(s) 79, 212
SpeI ACTAGT 1 cut(s) 520
Sse9I AATT 8 cut(s) 411, 451, 772, 781, 809, 884, 919, 945
SsiI CCGC 3 cut(s) 17, 103, 538
SspI AATATT 1 cut(s) 804
SspMI CTAG 5 cut(s) 6, 521, 687, 735, 824
SstI GAGCTC 1 cut(s) 272
StyD4I CCNGG 2 cut(s) 555, 693
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 1 cut(s) 569
TaiI ACGT 1 cut(s) 75
TaqI TCGA 5 cut(s) 141, 230, 254, 768, 807
TasI AATT 8 cut(s) 411, 451, 772, 781, 809, 884, 919, 945
TatI WGTACW 1 cut(s) 757
Tru1I TTAA 5 cut(s) 162, 483, 588, 609, 674
Tru9I TTAA 5 cut(s) 162, 483, 588, 609, 674
TscAI CASTG 1 cut(s) 574
TseI GCWGC 5 cut(s) 189, 373, 376, 471, 953
TspDTI ATGAA 3 cut(s) 446, 469, 894
TspRI CASTG 1 cut(s) 574
Tth111I GACNNNGTC 1 cut(s) 170
VneI GTGCAC 1 cut(s) 491
VpaK11BI GGWCC 1 cut(s) 667
XapI RAATTY 3 cut(s) 451, 809, 884
XcmI CCANNNNNNNNNTGG 1 cut(s) 280
XmiI GTMKAC 2 cut(s) 564, 768
XspI CTAG 5 cut(s) 6, 521, 687, 735, 824
Zsp2I ATGCAT 2 cut(s) 442, 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.