RLG00000021862

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018068
Physical Location & Seq
Forward (+)
78888219 .. 78896601
8383 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021862

Sequence Viewer

Length: 1527 bp
ATGGCCGCCTATTCAAGAATACAGGCATCGTCGTTTTCCTTCTATTCGTCAGCAACAAGGAGCAGGCACGACGTATTCTTGAGCTTTCGAGGCGAAGACACCCGCCACAACATAACCGAAAACCTCCACAAGGCCTTAGTCGACGCCGGCGTTCCCACGTTCAAAGACGATATTAATCTTCAGAAGGGCAACGAGATCGCGTCCGATCTGCTCAAGGCCATACAGATATCGAGAATCGCCATCATAGTGTTCTCCAACAACTACGCGAGCTCCAGCTGGTGTCTGGATGAGTTGGTCAAGATTGTAGAGTGCAAGAGGGAAGCGGGTCAGGTCATTTTGCCCATTTTCTGCGATGTGGATCCCTGTGATGTGCAGCAGCAGATGGGCACTTTTTCAGAAGCACTTGCTGTGCATGAAAGAAGTGGCAGGACCCAGAACAAGTTGGAGGCTTGGAAAGTGTCGTTGACAGAAGCGGCTAGTCAGCCTTATGGCTTTGATATGCGTGATGCTGCTACTTGGCTTCAATCGAAGTTCATAAAAGAAATTGTAAAGCATGTTTTGAGTAGATATCAAGATGCGCACTTGAGTGTTTCCATCTTCCCACCAAGATTAAACGCTGGTAAAGAATACATGAATTCCTTGTTAAGTGTAATATCAGATGACATTCTCATCATAGGCATCTGTGGAATGGAGAAAACTGCCGTGGCAAAAGATGCATATGACCAGAATTTTTATAGGTTTGAAAGCAGCAGTTTCATTAAAGATGTGAACAAGATGGCAGAACAACCCAATGGATTAGTTGCCTTACAAAAGCAGCTCCTTTCAGATATCCTGGGAGTAGACAATGTAGAAAATAATGATGTTAAGGTAGGAACTGAGTTGATTAAAGATGTAGCAAGAAGAAAAAGAGTGCTCATCTTTCTTGATAATGTGAATAAACTAGACCAGTTAAATGCACTTGCTAGGAGCCGGGAGTGGTTCGGCTCAGGAAGCAGAATAGTGATAACTACTAGAGATCCACAACTGCTGAGTGTACTTGAAGTCGATAATGTATATGGGACGCAAGGTATAGAGGTTATCGAGACACAGTTTCAGCAGAACCTTCAGATGATTAAAGACCTGGAGGAGCCTATCAGAAGGTCATATGTTCAGGCTCGTGATAAAGAAAGGTCCCTTATTGCTGAAATAGAAGCAATACGGAGAAAGAACGATGCTCAGGAGCAAATTATAGCAGAGCTGCAGCAAAATTTGAATTCGAGTTTCTCCTTGTATGCATTAGACTTTGCAACAGATGTTCTACTGGATGGAAGAGGGCCTGAAAAGTTTGAAGGATATGATGATTACAAAACCCTGCCCCGTTTTGTTCCTGAGAATGAGATGATAATTGGGGATGCTAATGTTAATGATGAGTTGAAGGCAAAGATGCAATTTCTTAATGTTGGATCCAAGGATTTCAATGAGGCAGTAAGCAGGGAATCATCCAGCCAGGCATTAATTCTGCGAGGGAGGAAGCAGGAACGTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

509

Amino Acids

57.49

Weight (kDa)

5.33

Isoelectric Point (pI)

48.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 191 6.5e-47 TIR domain
TIR_2 PF13676 25 - 123 2.3e-14 TIR domain
NB-ARC PF00931 222 - 343 1.8e-06 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018068)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49510
rosa_laevigata RLG00000021862
rosa_multiflora Rmu_sc0000648.1_g000014 Rmu_sc0035653.1_g000001
rosa_roxburghii Rroxscaffold_2G00082140 Rroxscaffold_2G00082170
rosa_rugosa Rorug02G0539900 Rorug02G0539900
rosa_samantha Rh2AG611200 Rh2BG624800
rosa_wichuraiana Rw2G050720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1525
Acc16I TGCGCA 1 cut(s) 579
AccI GTMKAC 2 cut(s) 141, 840
AccII CGCG 2 cut(s) 200, 266
AciI CCGC 4 cut(s) 6, 103, 323, 473
AclI AACGTT 1 cut(s) 1519
AclWI GGATC 5 cut(s) 353, 366, 1010, 1437, 1450
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 4 cut(s) 634, 727, 1246, 1252
AcuI CTGAAG 2 cut(s) 164, 1088
AcyI GRCGYC 1 cut(s) 144
AfaI GTAC 1 cut(s) 1035
AfiI CCNNNNNNNGG 1 cut(s) 130
AgsI TTSAA 9 cut(s) 15, 163, 524, 743, 1040, 1252, 1328, 1414, 1456
AjnI CCWGG 3 cut(s) 831, 1119, 1485
AleI CACNNNNGTG 1 cut(s) 585
AluBI AGCT 5 cut(s) 84, 270, 276, 817, 1237
AluI AGCT 5 cut(s) 84, 270, 276, 817, 1237
Alw21I GWGCWC 2 cut(s) 272, 915
Alw26I GTCTC 1 cut(s) 1076
AlwI GGATC 5 cut(s) 353, 366, 1010, 1437, 1450
AoxI GGCC 4 cut(s) 3, 132, 216, 1313
ApeKI GCWGC 7 cut(s) 373, 376, 509, 747, 814, 1237, 1240
ApoI RAATTY 4 cut(s) 634, 727, 1246, 1252
AseI ATTAAT 2 cut(s) 174, 1493
AspLEI GCGC 1 cut(s) 580
AspS9I GGNCC 3 cut(s) 429, 1170, 1313
AsuC2I CCSGG 1 cut(s) 971
AvaII GGWCC 2 cut(s) 429, 1170
BaeGI GKGCMC 1 cut(s) 389
BamHI GGATCC 2 cut(s) 358, 1442
BanII GRGCYC 1 cut(s) 272
BauI CACGAG 1 cut(s) 1155
BbsI GAAGAC 1 cut(s) 102
Bbv12I GWGCWC 2 cut(s) 272, 915
BbvI GCAGC 7 cut(s) 385, 388, 496, 759, 826, 1224, 1252
BccI CCATC 5 cut(s) 248, 376, 602, 769, 1298
BceAI ACGGC 1 cut(s) 686
BcgI CGANNNNNNTGC 2 cut(s) 178, 212
BciT130I CCWGG 3 cut(s) 833, 1121, 1487
BcnI CCSGG 1 cut(s) 971
BcoDI GTCTC 1 cut(s) 1076
BfaI CTAG 4 cut(s) 477, 941, 963, 1011
BfmI CTRYAG 1 cut(s) 1238
BisI GCNGC 9 cut(s) 6, 374, 377, 474, 510, 748, 815, 1238, 1241
BlsI GCNGC 9 cut(s) 7, 375, 378, 475, 511, 749, 816, 1239, 1242
Bme1390I CCNGG 4 cut(s) 833, 971, 1121, 1487
Bme18I GGWCC 2 cut(s) 429, 1170
BmgT120I GGNCC 3 cut(s) 429, 1170, 1313
BmiI GGNNCC 6 cut(s) 360, 431, 968, 1128, 1172, 1444
BmrFI CCNGG 4 cut(s) 833, 971, 1121, 1487
BmsI GCATC 8 cut(s) 35, 496, 565, 687, 703, 1201, 1381, 1413
BpiI GAAGAC 1 cut(s) 102
BpmI CTGGAG 2 cut(s) 256, 1142
Bpu10I CCTNAGC 2 cut(s) 985, 1215
BpuEI CTTGAG 3 cut(s) 100, 197, 604
BpuMI CCSGG 1 cut(s) 971
BsaBI GATNNNNATC 2 cut(s) 174, 357
BsaHI GRCGYC 1 cut(s) 144
BsaJI CCNNGG 3 cut(s) 702, 832, 1446
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 1 cut(s) 130
Bse118I RCCGGY 1 cut(s) 146
Bse1I ACTGG 2 cut(s) 946, 1305
Bse8I GATNNNNATC 2 cut(s) 174, 357
BseBI CCWGG 3 cut(s) 833, 1121, 1487
BseDI CCNNGG 3 cut(s) 702, 832, 1446
BseGI GGATG 4 cut(s) 292, 1309, 1396, 1478
BseJI GATNNNNATC 2 cut(s) 174, 357
BseLI CCNNNNNNNGG 1 cut(s) 130
BseMII CTCAG 5 cut(s) 867, 999, 1019, 1229, 1359
BseNI ACTGG 2 cut(s) 946, 1305
BseRI GAGGAG 1 cut(s) 1139
BseSI GKGCMC 1 cut(s) 389
BseXI GCAGC 7 cut(s) 385, 388, 496, 759, 826, 1224, 1252
BsgI GTGCAG 1 cut(s) 392
Bsh1236I CGCG 2 cut(s) 200, 266
BshFI GGCC 4 cut(s) 5, 134, 218, 1315
BsiHKAI GWGCWC 2 cut(s) 272, 915
BsiSI CCGG 2 cut(s) 147, 970
BslFI GGGAC 2 cut(s) 1072, 1156
BslI CCNNNNNNNGG 1 cut(s) 130
BsmAI GTCTC 1 cut(s) 1076
BsmFI GGGAC 2 cut(s) 1072, 1156
BsnI GGCC 4 cut(s) 5, 134, 218, 1315
Bsp1286I GDGCHC 3 cut(s) 272, 389, 915
Bsp143I GATC 5 cut(s) 195, 205, 358, 1015, 1442
BspACI CCGC 4 cut(s) 6, 103, 323, 473
BspANI GGCC 4 cut(s) 5, 134, 218, 1315
BspCNI CTCAG 5 cut(s) 868, 998, 1020, 1228, 1360
BspFNI CGCG 2 cut(s) 200, 266
BspLI GGNNCC 6 cut(s) 360, 431, 968, 1128, 1172, 1444
BspMAI CTGCAG 1 cut(s) 1242
BspPI GGATC 5 cut(s) 353, 366, 1010, 1437, 1450
BsrFI RCCGGY 1 cut(s) 146
BsrI ACTGG 2 cut(s) 946, 1305
BssAI RCCGGY 1 cut(s) 146
BssECI CCNNGG 3 cut(s) 702, 832, 1446
BssMI GATC 5 cut(s) 195, 205, 358, 1015, 1442
BssNI GRCGYC 1 cut(s) 144
BssSI CACGAG 1 cut(s) 1155
BssT1I CCWWGG 1 cut(s) 1446
Bst2BI CACGAG 1 cut(s) 1155
Bst2UI CCWGG 3 cut(s) 833, 1121, 1487
Bst4CI ACNGT 1 cut(s) 1089
Bst6I CTCTTC 1 cut(s) 1303
BstACI GRCGYC 1 cut(s) 144
BstAPI GCANNNNNTGC 1 cut(s) 713
BstC8I GCNNGC 3 cut(s) 65, 148, 268
BstDEI CTNAG 6 cut(s) 136, 876, 985, 1028, 1215, 1368
BstDSI CCRYGG 1 cut(s) 702
BstENI CCTNNNNNAGG 1 cut(s) 128
BstF5I GGATG 4 cut(s) 292, 1309, 1396, 1478
BstFNI CGCG 2 cut(s) 200, 266
BstHHI GCGC 1 cut(s) 580
BstKTI GATC 5 cut(s) 198, 208, 361, 1018, 1445
BstMAI GTCTC 1 cut(s) 1076
BstMBI GATC 5 cut(s) 195, 205, 358, 1015, 1442
BstMWI GCNNNNNNNGC 3 cut(s) 90, 713, 990
BstNI CCWGG 3 cut(s) 833, 1121, 1487
BstNSI RCATGY 1 cut(s) 557
BstSCI CCNGG 4 cut(s) 831, 969, 1119, 1485
BstSFI CTRYAG 1 cut(s) 1238
BstSLI GKGCMC 1 cut(s) 389
BstUI CGCG 2 cut(s) 200, 266
BstV1I GCAGC 7 cut(s) 385, 388, 496, 759, 826, 1224, 1252
BstV2I GAAGAC 1 cut(s) 102
BstX2I RGATCY 3 cut(s) 358, 1015, 1442
BstYI RGATCY 3 cut(s) 358, 1015, 1442
BsuRI GGCC 4 cut(s) 5, 134, 218, 1315
BtgI CCRYGG 1 cut(s) 702
BtgZI GCGATG 1 cut(s) 366
BtsCI GGATG 4 cut(s) 292, 1309, 1396, 1478
Cac8I GCNNGC 3 cut(s) 65, 148, 268
CfoI GCGC 1 cut(s) 580
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 3 cut(s) 429, 1170, 1313
CseI GACGC 3 cut(s) 152, 189, 1069
Csp6I GTAC 1 cut(s) 1034
CviAII CATG 3 cut(s) 413, 554, 631
CviQI GTAC 1 cut(s) 1034
DdeI CTNAG 6 cut(s) 136, 876, 985, 1028, 1215, 1368
DpnI GATC 5 cut(s) 197, 207, 360, 1017, 1444
DpnII GATC 5 cut(s) 195, 205, 358, 1015, 1442
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 1303
EarI CTCTTC 1 cut(s) 1303
Ecl136II GAGCTC 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 1446
Eco147I AGGCCT 1 cut(s) 134
Eco24I GRGCYC 1 cut(s) 272
Eco32I GATATC 3 cut(s) 228, 569, 829
Eco47I GGWCC 2 cut(s) 429, 1170
Eco53kI GAGCTC 1 cut(s) 270
Eco57I CTGAAG 2 cut(s) 164, 1088
EcoICRI GAGCTC 1 cut(s) 270
EcoNI CCTNNNNNAGG 1 cut(s) 128
EcoO109I RGGNCCY 3 cut(s) 429, 1170, 1313
EcoRI GAATTC 2 cut(s) 634, 1252
EcoRII CCWGG 3 cut(s) 831, 1119, 1485
EcoRV GATATC 3 cut(s) 228, 569, 829
EcoT14I CCWWGG 1 cut(s) 1446
EcoT22I ATGCAT 2 cut(s) 718, 1276
EcoT38I GRGCYC 1 cut(s) 272
ErhI CCWWGG 1 cut(s) 1446
FaeI CATG 3 cut(s) 416, 557, 634
FaqI GGGAC 2 cut(s) 1072, 1156
FatI CATG 3 cut(s) 412, 553, 630
FauI CCCGC 2 cut(s) 110, 316
FauNDI CATATG 2 cut(s) 718, 1144
FblI GTMKAC 2 cut(s) 141, 840
Fnu4HI GCNGC 9 cut(s) 6, 374, 377, 474, 510, 748, 815, 1238, 1241
FokI GGATG 4 cut(s) 299, 1316, 1403, 1465
FriOI GRGCYC 1 cut(s) 272
Fsp4HI GCNGC 9 cut(s) 6, 374, 377, 474, 510, 748, 815, 1238, 1241
FspAI RTGCGCAY 1 cut(s) 579
FspBI CTAG 4 cut(s) 477, 941, 963, 1011
FspI TGCGCA 1 cut(s) 579
GlaI GCGC 1 cut(s) 579
GluI GCNGC 9 cut(s) 6, 374, 377, 474, 510, 748, 815, 1238, 1241
GsuI CTGGAG 2 cut(s) 256, 1142
HaeIII GGCC 4 cut(s) 5, 134, 218, 1315
HapII CCGG 2 cut(s) 147, 970
HgaI GACGC 3 cut(s) 152, 189, 1069
HhaI GCGC 1 cut(s) 580
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 3 cut(s) 416, 557, 634
Hin6I GCGC 1 cut(s) 578
HinP1I GCGC 1 cut(s) 578
HincII GTYRAC 2 cut(s) 142, 465
HindII GTYRAC 2 cut(s) 142, 465
HinfI GANTC 2 cut(s) 234, 1475
HpaII CCGG 2 cut(s) 147, 970
Hpy166II GTNNAC 5 cut(s) 142, 465, 769, 841, 1034
Hpy188I TCNGA 7 cut(s) 183, 205, 397, 658, 826, 1107, 1136
Hpy8I GTNNAC 5 cut(s) 142, 465, 769, 841, 1034
Hpy99I CGWCG 3 cut(s) 34, 74, 146
HpyAV CCTTC 6 cut(s) 49, 178, 1112, 1131, 1322, 1408
HpyCH4III ACNGT 1 cut(s) 1089
HpyCH4IV ACGT 3 cut(s) 72, 158, 1519
HpyCH4V TGCA 9 cut(s) 312, 373, 412, 716, 956, 1240, 1274, 1286, 1426
HpyF10VI GCNNNNNNNGC 3 cut(s) 90, 713, 990
HpyF3I CTNAG 6 cut(s) 136, 876, 985, 1028, 1215, 1368
HpySE526I ACGT 3 cut(s) 72, 158, 1519
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 3 cut(s) 416, 557, 634
HspAI GCGC 1 cut(s) 578
KroI GCCGGC 1 cut(s) 146
KroNI GCCGGC 1 cut(s) 148
Kzo9I GATC 5 cut(s) 195, 205, 358, 1015, 1442
LmnI GCTCC 6 cut(s) 60, 275, 822, 966, 1126, 1219
Lsp1109I GCAGC 7 cut(s) 385, 388, 496, 759, 826, 1224, 1252
LweI GCATC 8 cut(s) 35, 496, 565, 687, 703, 1201, 1381, 1413
MaeI CTAG 4 cut(s) 477, 941, 963, 1011
MaeII ACGT 3 cut(s) 72, 158, 1519
MalI GATC 5 cut(s) 197, 207, 360, 1017, 1444
MboI GATC 5 cut(s) 195, 205, 358, 1015, 1442
MboII GAAGA 5 cut(s) 107, 170, 589, 912, 1320
MflI RGATCY 3 cut(s) 358, 1015, 1442
MhlI GDGCHC 3 cut(s) 272, 389, 915
MluCI AATT 9 cut(s) 543, 634, 727, 1224, 1246, 1252, 1383, 1427, 1494
MmeI TCCRAC 3 cut(s) 279, 423, 1420
Mph1103I ATGCAT 2 cut(s) 718, 1276
MreI CGCCGGCG 1 cut(s) 146
MroNI GCCGGC 1 cut(s) 146
MslI CAYNNNNRTG 2 cut(s) 245, 585
MspA1I CMGCKG 1 cut(s) 276
MspI CCGG 2 cut(s) 147, 970
MspR9I CCNGG 4 cut(s) 833, 971, 1121, 1487
MvaI CCWGG 3 cut(s) 833, 1121, 1487
MvnI CGCG 2 cut(s) 200, 266
MwoI GCNNNNNNNGC 3 cut(s) 90, 713, 990
NaeI GCCGGC 1 cut(s) 148
NciI CCSGG 1 cut(s) 971
NdeI CATATG 2 cut(s) 718, 1144
NdeII GATC 5 cut(s) 195, 205, 358, 1015, 1442
NgoMIV GCCGGC 1 cut(s) 146
NlaIII CATG 3 cut(s) 416, 557, 634
NlaIV GGNNCC 6 cut(s) 360, 431, 968, 1128, 1172, 1444
NsbI TGCGCA 1 cut(s) 579
NsiI ATGCAT 2 cut(s) 718, 1276
NspI RCATGY 1 cut(s) 557
OliI CACNNNNGTG 1 cut(s) 585
PceI AGGCCT 1 cut(s) 134
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PdiI GCCGGC 1 cut(s) 148
PfeI GAWTC 2 cut(s) 234, 1475
PkrI GCNGC 9 cut(s) 7, 375, 378, 475, 511, 749, 816, 1239, 1242
PpuMI RGGWCCY 2 cut(s) 429, 1170
PshBI ATTAAT 2 cut(s) 174, 1493
PsiI TTATAA 1 cut(s) 1525
Psp124BI GAGCTC 1 cut(s) 272
Psp1406I AACGTT 1 cut(s) 1519
Psp5II RGGWCCY 2 cut(s) 429, 1170
Psp6I CCWGG 3 cut(s) 831, 1119, 1485
PspGI CCWGG 3 cut(s) 831, 1119, 1485
PspN4I GGNNCC 6 cut(s) 360, 431, 968, 1128, 1172, 1444
PspPI GGNCC 3 cut(s) 429, 1170, 1313
PspPPI RGGWCCY 2 cut(s) 429, 1170
PstI CTGCAG 1 cut(s) 1242
PsuI RGATCY 3 cut(s) 358, 1015, 1442
PvuII CAGCTG 1 cut(s) 276
RsaI GTAC 1 cut(s) 1035
RsaNI GTAC 1 cut(s) 1034
RseI CAYNNNNRTG 2 cut(s) 245, 585
SacI GAGCTC 1 cut(s) 272
SalI GTCGAC 1 cut(s) 140
SatI GCNGC 9 cut(s) 6, 374, 377, 474, 510, 748, 815, 1238, 1241
Sau3AI GATC 5 cut(s) 195, 205, 358, 1015, 1442
Sau96I GGNCC 3 cut(s) 429, 1170, 1313
ScrFI CCNGG 4 cut(s) 833, 971, 1121, 1487
SduI GDGCHC 3 cut(s) 272, 389, 915
SfaNI GCATC 8 cut(s) 35, 496, 565, 687, 703, 1201, 1381, 1413
SfcI CTRYAG 1 cut(s) 1238
SgrAI CRCCGGYG 1 cut(s) 146
SinI GGWCC 2 cut(s) 429, 1170
SmiMI CAYNNNNRTG 2 cut(s) 245, 585
SmlI CTYRAG 3 cut(s) 79, 212, 583
SmoI CTYRAG 3 cut(s) 79, 212, 583
Sse9I AATT 9 cut(s) 543, 634, 727, 1224, 1246, 1252, 1383, 1427, 1494
SseBI AGGCCT 1 cut(s) 134
SsiI CCGC 4 cut(s) 6, 103, 323, 473
SspMI CTAG 4 cut(s) 477, 941, 963, 1011
SstI GAGCTC 1 cut(s) 272
StuI AGGCCT 1 cut(s) 134
StyD4I CCNGG 4 cut(s) 831, 969, 1119, 1485
StyI CCWWGG 1 cut(s) 1446
TaaI ACNGT 1 cut(s) 1089
TaiI ACGT 3 cut(s) 75, 161, 1522
TaqI TCGA 7 cut(s) 88, 141, 230, 527, 1044, 1080, 1256
TasI AATT 9 cut(s) 543, 634, 727, 1224, 1246, 1252, 1383, 1427, 1494
TatI WGTACW 1 cut(s) 1033
TauI GCSGC 2 cut(s) 8, 476
TfiI GAWTC 2 cut(s) 234, 1475
TseI GCWGC 7 cut(s) 373, 376, 509, 747, 814, 1237, 1240
TspDTI ATGAA 4 cut(s) 429, 523, 647, 745
TspGWI ACGGA 1 cut(s) 1213
VpaK11BI GGWCC 2 cut(s) 429, 1170
VspI ATTAAT 2 cut(s) 174, 1493
XagI CCTNNNNNAGG 1 cut(s) 128
XapI RAATTY 4 cut(s) 634, 727, 1246, 1252
XceI RCATGY 1 cut(s) 557
XcmI CCANNNNNNNNNTGG 1 cut(s) 280
XmiI GTMKAC 2 cut(s) 141, 840
XspI CTAG 4 cut(s) 477, 941, 963, 1011
Zsp2I ATGCAT 2 cut(s) 718, 1276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.