Rmu_sc0000648.1_g000014

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000648.1
OG0018068
Physical Location & Seq
Forward (+)
67520 .. 69027
1508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000648.1_g000014.1.cds

Sequence Viewer

Length: 1098 bp
atggccgcctatacaagaatacaggcatcgtcgttttccttctattcgtcagcaacagggcgcaggcacgacgtattcttgagctttcgaggcgaagacacccgccacaacataaccgaaaacctccacaaggccttagtcgacgccggcgttcccacgttcaaagacgatgttaaccttcagaagggcaacgagattgcgtccgatctgctcaaggccatacagacatcgagaatcgccatcatagtgttctccaacaactacgcgagctctagctggtgtctggatgagttggtcaagattgtagagtgcaacagggaagcgggtcaggtcattttgcccattttctgcgatgtggatccctgtgatgtgcagcagcagatgggcactttttcagaagcacttgctgtgcatgaaagaagtggcaggacccaggacaagttggaggcttggaaagtgtcgttgaccgaagcggctagtcagccttatggctttgatatgcgggatgctgctacttggcttcaatcgaagttcataaaagaaattgtaaagcatgttctgagtagatatcaagatgcgcacttgagtgtttccatcttcccaccaagattagatgctggtaaagaatacatgaattccttgttaagtgtaatatcagatgacattctcatcataggcatctgtggaatggagaaaactgccttggcaaaagatgcgtatgaccagaatttttataggtttgaaagcagcagtttcattaaagatgtgaacaagatggcagaacaacccaatggattagttgccttgcaaaagtggctcctttcagatatcctgggagtagacaatgtagaaataaatgatgttaaggaaggaactgagttgattaaagatgtagcaagaagaaaaagagtgctcatctttcttgataatgtgaataaactagaccagttaaatgcactcgctaggagacgggagtggttcggctcaggaagcagaatagtgataactactagagatccacaactgctaagtgtacttgaagtcgataatgtatatgggacgcaaggcatagaggtaaaattttgctctgtgctcaaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

366

Amino Acids

41.19

Weight (kDa)

5.65

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018068)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49510
rosa_laevigata RLG00000021862
rosa_multiflora Rmu_sc0000648.1_g000014 Rmu_sc0035653.1_g000001
rosa_roxburghii Rroxscaffold_2G00082140 Rroxscaffold_2G00082170
rosa_rugosa Rorug02G0539900 Rorug02G0539900
rosa_samantha Rh2AG611200 Rh2BG624800
rosa_wichuraiana Rw2G050720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 579
AccI GTMKAC 2 cut(s) 141, 840
AccII CGCG 1 cut(s) 266
AciI CCGC 5 cut(s) 6, 103, 323, 473, 502
AclWI GGATC 3 cut(s) 353, 366, 1010
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 634, 727, 1079
AcuI CTGAAG 1 cut(s) 164
AcyI GRCGYC 1 cut(s) 144
AfaI GTAC 1 cut(s) 1035
AfiI CCNNNNNNNGG 2 cut(s) 130, 184
AgsI TTSAA 4 cut(s) 163, 524, 743, 1040
AjnI CCWGG 2 cut(s) 432, 831
AjuI GAANNNNNNNTTGG 2 cut(s) 686, 718
AleI CACNNNNGTG 1 cut(s) 585
AluBI AGCT 3 cut(s) 84, 270, 276
AluI AGCT 3 cut(s) 84, 270, 276
Alw21I GWGCWC 3 cut(s) 272, 915, 1095
Alw26I GTCTC 1 cut(s) 961
AlwI GGATC 3 cut(s) 353, 366, 1010
AoxI GGCC 3 cut(s) 3, 132, 216
ApeKI GCWGC 4 cut(s) 373, 376, 509, 747
ApoI RAATTY 3 cut(s) 634, 727, 1079
AspLEI GCGC 2 cut(s) 63, 580
AspS9I GGNCC 1 cut(s) 429
AvaII GGWCC 1 cut(s) 429
BaeGI GKGCMC 1 cut(s) 389
BamHI GGATCC 1 cut(s) 358
BanII GRGCYC 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 102
Bbv12I GWGCWC 3 cut(s) 272, 915, 1095
BbvI GCAGC 4 cut(s) 385, 388, 496, 759
BccI CCATC 4 cut(s) 248, 376, 602, 769
BciT130I CCWGG 2 cut(s) 434, 833
BcoDI GTCTC 1 cut(s) 961
BfaI CTAG 5 cut(s) 273, 477, 941, 963, 1011
BisI GCNGC 6 cut(s) 6, 374, 377, 474, 510, 748
BlsI GCNGC 6 cut(s) 7, 375, 378, 475, 511, 749
Bme1390I CCNGG 2 cut(s) 434, 833
Bme18I GGWCC 1 cut(s) 429
BmgT120I GGNCC 1 cut(s) 429
BmiI GGNNCC 3 cut(s) 360, 431, 818
BmrFI CCNGG 2 cut(s) 434, 833
BmsI GCATC 6 cut(s) 35, 496, 565, 604, 687, 703
BpiI GAAGAC 1 cut(s) 102
Bpu10I CCTNAGC 1 cut(s) 985
BpuEI CTTGAG 3 cut(s) 100, 197, 604
BsaBI GATNNNNATC 1 cut(s) 357
BsaHI GRCGYC 1 cut(s) 144
BsaJI CCNNGG 3 cut(s) 432, 702, 832
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 184
Bse118I RCCGGY 1 cut(s) 146
Bse1I ACTGG 1 cut(s) 946
Bse8I GATNNNNATC 1 cut(s) 357
BseBI CCWGG 2 cut(s) 434, 833
BseDI CCNNGG 3 cut(s) 432, 702, 832
BseGI GGATG 2 cut(s) 292, 511
BseJI GATNNNNATC 1 cut(s) 357
BseLI CCNNNNNNNGG 2 cut(s) 130, 184
BseMII CTCAG 3 cut(s) 551, 867, 999
BseNI ACTGG 1 cut(s) 946
BseSI GKGCMC 1 cut(s) 389
BseXI GCAGC 4 cut(s) 385, 388, 496, 759
BsgI GTGCAG 1 cut(s) 392
Bsh1236I CGCG 1 cut(s) 266
BshFI GGCC 3 cut(s) 5, 134, 218
BsiHKAI GWGCWC 3 cut(s) 272, 915, 1095
BsiSI CCGG 1 cut(s) 147
BslFI GGGAC 1 cut(s) 1072
BslI CCNNNNNNNGG 2 cut(s) 130, 184
BsmAI GTCTC 1 cut(s) 961
BsmBI CGTCTC 1 cut(s) 961
BsmFI GGGAC 1 cut(s) 1072
BsnI GGCC 3 cut(s) 5, 134, 218
Bsp1286I GDGCHC 4 cut(s) 272, 389, 915, 1095
Bsp143I GATC 3 cut(s) 205, 358, 1015
BspACI CCGC 5 cut(s) 6, 103, 323, 473, 502
BspANI GGCC 3 cut(s) 5, 134, 218
BspCNI CTCAG 3 cut(s) 552, 868, 998
BspFNI CGCG 1 cut(s) 266
BspLI GGNNCC 3 cut(s) 360, 431, 818
BspPI GGATC 3 cut(s) 353, 366, 1010
BsrFI RCCGGY 1 cut(s) 146
BsrI ACTGG 1 cut(s) 946
BssAI RCCGGY 1 cut(s) 146
BssECI CCNNGG 3 cut(s) 432, 702, 832
BssMI GATC 3 cut(s) 205, 358, 1015
BssNI GRCGYC 1 cut(s) 144
BssT1I CCWWGG 1 cut(s) 702
Bst2UI CCWGG 2 cut(s) 434, 833
BstACI GRCGYC 1 cut(s) 144
BstAPI GCANNNNNTGC 1 cut(s) 713
BstC8I GCNNGC 3 cut(s) 65, 148, 268
BstDEI CTNAG 5 cut(s) 136, 560, 876, 985, 1028
BstENI CCTNNNNNAGG 2 cut(s) 128, 182
BstF5I GGATG 2 cut(s) 292, 511
BstFNI CGCG 1 cut(s) 266
BstHHI GCGC 2 cut(s) 63, 580
BstKTI GATC 3 cut(s) 208, 361, 1018
BstMAI GTCTC 1 cut(s) 961
BstMBI GATC 3 cut(s) 205, 358, 1015
BstMWI GCNNNNNNNGC 4 cut(s) 90, 713, 814, 990
BstNI CCWGG 2 cut(s) 434, 833
BstNSI RCATGY 1 cut(s) 557
BstSCI CCNGG 2 cut(s) 432, 831
BstSLI GKGCMC 1 cut(s) 389
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 4 cut(s) 385, 388, 496, 759
BstV2I GAAGAC 1 cut(s) 102
BstX2I RGATCY 2 cut(s) 358, 1015
BstYI RGATCY 2 cut(s) 358, 1015
BsuRI GGCC 3 cut(s) 5, 134, 218
BtgZI GCGATG 1 cut(s) 366
BtsCI GGATG 2 cut(s) 292, 511
Cac8I GCNNGC 3 cut(s) 65, 148, 268
CfoI GCGC 2 cut(s) 63, 580
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 1 cut(s) 429
CseI GACGC 3 cut(s) 152, 189, 1069
Csp6I GTAC 1 cut(s) 1034
CviAII CATG 3 cut(s) 413, 554, 631
CviQI GTAC 1 cut(s) 1034
DdeI CTNAG 5 cut(s) 136, 560, 876, 985, 1028
DpnI GATC 3 cut(s) 207, 360, 1017
DpnII GATC 3 cut(s) 205, 358, 1015
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 702
Eco147I AGGCCT 1 cut(s) 134
Eco24I GRGCYC 1 cut(s) 272
Eco32I GATATC 2 cut(s) 569, 829
Eco47I GGWCC 1 cut(s) 429
Eco53kI GAGCTC 1 cut(s) 270
Eco57I CTGAAG 1 cut(s) 164
EcoICRI GAGCTC 1 cut(s) 270
EcoNI CCTNNNNNAGG 2 cut(s) 128, 182
EcoO109I RGGNCCY 1 cut(s) 429
EcoRI GAATTC 1 cut(s) 634
EcoRII CCWGG 2 cut(s) 432, 831
EcoRV GATATC 2 cut(s) 569, 829
EcoT14I CCWWGG 1 cut(s) 702
EcoT38I GRGCYC 1 cut(s) 272
ErhI CCWWGG 1 cut(s) 702
Esp3I CGTCTC 1 cut(s) 961
FaeI CATG 3 cut(s) 416, 557, 634
FaqI GGGAC 1 cut(s) 1072
FatI CATG 3 cut(s) 412, 553, 630
FauI CCCGC 3 cut(s) 110, 316, 495
FblI GTMKAC 2 cut(s) 141, 840
Fnu4HI GCNGC 6 cut(s) 6, 374, 377, 474, 510, 748
FokI GGATG 2 cut(s) 299, 518
FriOI GRGCYC 1 cut(s) 272
Fsp4HI GCNGC 6 cut(s) 6, 374, 377, 474, 510, 748
FspAI RTGCGCAY 1 cut(s) 579
FspBI CTAG 5 cut(s) 273, 477, 941, 963, 1011
FspI TGCGCA 1 cut(s) 579
GlaI GCGC 2 cut(s) 62, 579
GluI GCNGC 6 cut(s) 6, 374, 377, 474, 510, 748
HaeIII GGCC 3 cut(s) 5, 134, 218
HapII CCGG 1 cut(s) 147
HgaI GACGC 3 cut(s) 152, 189, 1069
HhaI GCGC 2 cut(s) 63, 580
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 3 cut(s) 416, 557, 634
Hin6I GCGC 2 cut(s) 61, 578
HinP1I GCGC 2 cut(s) 61, 578
HincII GTYRAC 3 cut(s) 142, 175, 465
HindII GTYRAC 3 cut(s) 142, 175, 465
HinfI GANTC 1 cut(s) 234
HpaI GTTAAC 1 cut(s) 175
HpaII CCGG 1 cut(s) 147
Hpy166II GTNNAC 6 cut(s) 142, 175, 465, 769, 841, 1034
Hpy188I TCNGA 6 cut(s) 183, 205, 397, 561, 658, 826
Hpy188III TCNNGA 7 cut(s) 79, 231, 284, 298, 572, 923, 987
Hpy8I GTNNAC 6 cut(s) 142, 175, 465, 769, 841, 1034
Hpy99I CGWCG 3 cut(s) 34, 74, 146
HpyAV CCTTC 4 cut(s) 49, 178, 188, 863
HpyCH4IV ACGT 2 cut(s) 72, 158
HpyCH4V TGCA 5 cut(s) 312, 373, 412, 808, 956
HpyF10VI GCNNNNNNNGC 4 cut(s) 90, 713, 814, 990
HpyF3I CTNAG 5 cut(s) 136, 560, 876, 985, 1028
HpySE526I ACGT 2 cut(s) 72, 158
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 3 cut(s) 416, 557, 634
HspAI GCGC 2 cut(s) 61, 578
KroI GCCGGC 1 cut(s) 146
KroNI GCCGGC 1 cut(s) 148
KspAI GTTAAC 1 cut(s) 175
Kzo9I GATC 3 cut(s) 205, 358, 1015
LmnI GCTCC 1 cut(s) 822
Lsp1109I GCAGC 4 cut(s) 385, 388, 496, 759
LweI GCATC 6 cut(s) 35, 496, 565, 604, 687, 703
MaeI CTAG 5 cut(s) 273, 477, 941, 963, 1011
MaeII ACGT 2 cut(s) 72, 158
MalI GATC 3 cut(s) 207, 360, 1017
MboI GATC 3 cut(s) 205, 358, 1015
MboII GAAGA 3 cut(s) 107, 589, 912
MflI RGATCY 2 cut(s) 358, 1015
MhlI GDGCHC 4 cut(s) 272, 389, 915, 1095
MluCI AATT 4 cut(s) 543, 634, 727, 1079
MmeI TCCRAC 2 cut(s) 279, 423
MnlI CCTC 4 cut(s) 83, 134, 439, 1066
MreI CGCCGGCG 1 cut(s) 146
MroNI GCCGGC 1 cut(s) 146
MseI TTAA 6 cut(s) 174, 644, 759, 864, 885, 950
MslI CAYNNNNRTG 2 cut(s) 245, 585
MspI CCGG 1 cut(s) 147
MspR9I CCNGG 2 cut(s) 434, 833
MvaI CCWGG 2 cut(s) 434, 833
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 4 cut(s) 90, 713, 814, 990
NaeI GCCGGC 1 cut(s) 148
NdeII GATC 3 cut(s) 205, 358, 1015
NgoMIV GCCGGC 1 cut(s) 146
NlaIII CATG 3 cut(s) 416, 557, 634
NlaIV GGNNCC 3 cut(s) 360, 431, 818
NsbI TGCGCA 1 cut(s) 579
NspI RCATGY 1 cut(s) 557
OliI CACNNNNGTG 1 cut(s) 585
PceI AGGCCT 1 cut(s) 134
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PdiI GCCGGC 1 cut(s) 148
PfeI GAWTC 1 cut(s) 234
PkrI GCNGC 6 cut(s) 7, 375, 378, 475, 511, 749
PpuMI RGGWCCY 1 cut(s) 429
Psp124BI GAGCTC 1 cut(s) 272
Psp5II RGGWCCY 1 cut(s) 429
Psp6I CCWGG 2 cut(s) 432, 831
PspGI CCWGG 2 cut(s) 432, 831
PspN4I GGNNCC 3 cut(s) 360, 431, 818
PspPI GGNCC 1 cut(s) 429
PspPPI RGGWCCY 1 cut(s) 429
PsrI GAACNNNNNNTAC 2 cut(s) 540, 572
PsuI RGATCY 2 cut(s) 358, 1015
RsaI GTAC 1 cut(s) 1035
RsaNI GTAC 1 cut(s) 1034
RseI CAYNNNNRTG 2 cut(s) 245, 585
SacI GAGCTC 1 cut(s) 272
SalI GTCGAC 1 cut(s) 140
SaqAI TTAA 6 cut(s) 174, 644, 759, 864, 885, 950
SatI GCNGC 6 cut(s) 6, 374, 377, 474, 510, 748
Sau3AI GATC 3 cut(s) 205, 358, 1015
Sau96I GGNCC 1 cut(s) 429
ScrFI CCNGG 2 cut(s) 434, 833
SduI GDGCHC 4 cut(s) 272, 389, 915, 1095
SfaNI GCATC 6 cut(s) 35, 496, 565, 604, 687, 703
SgrAI CRCCGGYG 1 cut(s) 146
SinI GGWCC 1 cut(s) 429
SmiMI CAYNNNNRTG 2 cut(s) 245, 585
SmlI CTYRAG 3 cut(s) 79, 212, 583
SmoI CTYRAG 3 cut(s) 79, 212, 583
Sse9I AATT 4 cut(s) 543, 634, 727, 1079
SseBI AGGCCT 1 cut(s) 134
SsiI CCGC 5 cut(s) 6, 103, 323, 473, 502
SspMI CTAG 5 cut(s) 273, 477, 941, 963, 1011
SstI GAGCTC 1 cut(s) 272
StuI AGGCCT 1 cut(s) 134
StyD4I CCNGG 2 cut(s) 432, 831
StyI CCWWGG 1 cut(s) 702
TaiI ACGT 2 cut(s) 75, 161
TaqI TCGA 5 cut(s) 88, 141, 230, 527, 1044
TaqII GACCGA 1 cut(s) 482
TasI AATT 4 cut(s) 543, 634, 727, 1079
TatI WGTACW 1 cut(s) 1033
TauI GCSGC 2 cut(s) 8, 476
TfiI GAWTC 1 cut(s) 234
Tru1I TTAA 6 cut(s) 174, 644, 759, 864, 885, 950
Tru9I TTAA 6 cut(s) 174, 644, 759, 864, 885, 950
TseI GCWGC 4 cut(s) 373, 376, 509, 747
TspDTI ATGAA 4 cut(s) 429, 523, 647, 745
VpaK11BI GGWCC 1 cut(s) 429
XagI CCTNNNNNAGG 2 cut(s) 128, 182
XapI RAATTY 3 cut(s) 634, 727, 1079
XceI RCATGY 1 cut(s) 557
XmiI GTMKAC 2 cut(s) 141, 840
XspI CTAG 5 cut(s) 273, 477, 941, 963, 1011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.