Rh2BG624800

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0018068
Physical Location & Seq
Forward (+)
84674006 .. 84674621
616 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG624800.1

Sequence Viewer

Length: 462 bp
ATGCAGGTTATCGAGACACAGTTTCAGCAGAACCTTCAGATGATTAAAGACCTGGAGGAGCCTATCAGAAGGTCATATGTTCAGGCTCGTGATAAAGAAAGGTCCCTTATTGCTGAAATAGAAGCAATACGGAGAAAGAACGATGCTCAGGCTCAAATTATAGCAGAGCTGCAGCAAACTTCGAATTCGAGTTTCCCCTTGTACCCATTAGACTCCACAACAGATGTTCTACTGGATGGAAGAAGGCCTGAATATTTTGAAGGATATGATGATGACAAAACCCTGCTCAGTTTTGTACCTGAGAATGAGATGATGATAACTGGGGATGCTAATGTTAATGATGAGTTGAAGGCAAAAATGCATTTTCTTAATATTGGATCCAAGTATCTGAATGAAGCAGGAAGCAGTGAATCATCCAGCAAGGCATTAATTCTGCGAGGGAGGAAGCAGGAACTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

153

Amino Acids

17.54

Weight (kDa)

4.83

Isoelectric Point (pI)

68.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018068)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49510
rosa_laevigata RLG00000021862
rosa_multiflora Rmu_sc0000648.1_g000014 Rmu_sc0035653.1_g000001
rosa_roxburghii Rroxscaffold_2G00082140 Rroxscaffold_2G00082170
rosa_rugosa Rorug02G0539900 Rorug02G0539900
rosa_samantha Rh2AG611200 Rh2BG624800
rosa_wichuraiana Rw2G050720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 372, 385
AcsI RAATTY 1 cut(s) 184
AcuI CTGAAG 1 cut(s) 20
AfaI GTAC 2 cut(s) 203, 297
AgsI TTSAA 2 cut(s) 260, 349
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 1 cut(s) 169
AluI AGCT 1 cut(s) 169
Alw26I GTCTC 1 cut(s) 8
AlwI GGATC 2 cut(s) 372, 385
AoxI GGCC 1 cut(s) 245
ApeKI GCWGC 2 cut(s) 169, 172
ApoI RAATTY 1 cut(s) 184
AseI ATTAAT 1 cut(s) 428
AspS9I GGNCC 1 cut(s) 102
AsuII TTCGAA 1 cut(s) 182
AvaII GGWCC 1 cut(s) 102
BamHI GGATCC 1 cut(s) 377
BauI CACGAG 1 cut(s) 87
BbvI GCAGC 2 cut(s) 156, 184
BccI CCATC 1 cut(s) 230
BciT130I CCWGG 1 cut(s) 53
BcoDI GTCTC 1 cut(s) 8
BfmI CTRYAG 1 cut(s) 170
BisI GCNGC 2 cut(s) 170, 173
BlsI GCNGC 2 cut(s) 171, 174
Bme1390I CCNGG 1 cut(s) 53
Bme18I GGWCC 1 cut(s) 102
BmgT120I GGNCC 1 cut(s) 102
BmiI GGNNCC 3 cut(s) 60, 104, 379
BmrFI CCNGG 1 cut(s) 53
BmrI ACTGGG 1 cut(s) 330
BmsI GCATC 2 cut(s) 133, 316
BmuI ACTGGG 1 cut(s) 330
BpmI CTGGAG 1 cut(s) 74
Bpu10I CCTNAGC 1 cut(s) 147
Bpu14I TTCGAA 1 cut(s) 182
Bse1I ACTGG 2 cut(s) 237, 325
BseBI CCWGG 1 cut(s) 53
BseGI GGATG 3 cut(s) 241, 331, 413
BseMII CTCAG 3 cut(s) 161, 291, 301
BseNI ACTGG 2 cut(s) 237, 325
BseRI GAGGAG 1 cut(s) 71
BseXI GCAGC 2 cut(s) 156, 184
BshFI GGCC 1 cut(s) 247
BslFI GGGAC 1 cut(s) 88
BsmAI GTCTC 1 cut(s) 8
BsmFI GGGAC 1 cut(s) 88
BsnI GGCC 1 cut(s) 247
Bsp119I TTCGAA 1 cut(s) 182
Bsp143I GATC 1 cut(s) 377
BspANI GGCC 1 cut(s) 247
BspCNI CTCAG 3 cut(s) 160, 292, 300
BspLI GGNNCC 3 cut(s) 60, 104, 379
BspMAI CTGCAG 1 cut(s) 174
BspPI GGATC 2 cut(s) 372, 385
BspT104I TTCGAA 1 cut(s) 182
BsrI ACTGG 2 cut(s) 237, 325
BssMI GATC 1 cut(s) 377
BssSI CACGAG 1 cut(s) 87
Bst2BI CACGAG 1 cut(s) 87
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 1 cut(s) 21
BstBI TTCGAA 1 cut(s) 182
BstDEI CTNAG 3 cut(s) 147, 287, 300
BstF5I GGATG 3 cut(s) 241, 331, 413
BstKTI GATC 1 cut(s) 380
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 1 cut(s) 377
BstNI CCWGG 1 cut(s) 53
BstSCI CCNGG 1 cut(s) 51
BstSFI CTRYAG 1 cut(s) 170
BstV1I GCAGC 2 cut(s) 156, 184
BstX2I RGATCY 1 cut(s) 377
BstYI RGATCY 1 cut(s) 377
BsuRI GGCC 1 cut(s) 247
BtsCI GGATG 3 cut(s) 241, 331, 413
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 1 cut(s) 412
Cfr13I GGNCC 1 cut(s) 102
Csp6I GTAC 2 cut(s) 202, 296
CviJI RGCY 5 cut(s) 61, 86, 152, 169, 247
CviKI_1 RGCY 5 cut(s) 61, 86, 152, 169, 247
CviQI GTAC 2 cut(s) 202, 296
DdeI CTNAG 3 cut(s) 147, 287, 300
DpnI GATC 1 cut(s) 379
DpnII GATC 1 cut(s) 377
Eco147I AGGCCT 1 cut(s) 247
Eco47I GGWCC 1 cut(s) 102
Eco57I CTGAAG 1 cut(s) 20
EcoO109I RGGNCCY 1 cut(s) 102
EcoRI GAATTC 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 51
EcoT22I ATGCAT 1 cut(s) 363
FaiI YATR 4 cut(s) 76, 78, 161, 267
FaqI GGGAC 1 cut(s) 88
FauNDI CATATG 1 cut(s) 76
Fnu4HI GCNGC 2 cut(s) 170, 173
FokI GGATG 3 cut(s) 248, 338, 400
Fsp4HI GCNGC 2 cut(s) 170, 173
GluI GCNGC 2 cut(s) 170, 173
GsuI CTGGAG 1 cut(s) 74
HaeIII GGCC 1 cut(s) 247
HinfI GANTC 2 cut(s) 212, 410
Hpy188I TCNGA 3 cut(s) 39, 68, 390
Hpy188III TCNNGA 2 cut(s) 13, 89
HpyAV CCTTC 5 cut(s) 44, 63, 237, 254, 343
HpyCH4III ACNGT 1 cut(s) 21
HpyCH4V TGCA 3 cut(s) 4, 172, 361
HpyF3I CTNAG 3 cut(s) 147, 287, 300
Kzo9I GATC 1 cut(s) 377
LmnI GCTCC 1 cut(s) 58
Lsp1109I GCAGC 2 cut(s) 156, 184
LweI GCATC 2 cut(s) 133, 316
MalI GATC 1 cut(s) 379
MboI GATC 1 cut(s) 377
MboII GAAGA 1 cut(s) 252
MflI RGATCY 1 cut(s) 377
MluCI AATT 3 cut(s) 156, 184, 429
MlyI GAGTC 1 cut(s) 206
MnlI CCTC 3 cut(s) 49, 431, 435
Mph1103I ATGCAT 1 cut(s) 363
MseI TTAA 4 cut(s) 45, 336, 369, 428
MspR9I CCNGG 1 cut(s) 53
MvaI CCWGG 1 cut(s) 53
NdeI CATATG 1 cut(s) 76
NdeII GATC 1 cut(s) 377
NlaIV GGNNCC 3 cut(s) 60, 104, 379
NsiI ATGCAT 1 cut(s) 363
NspV TTCGAA 1 cut(s) 182
PceI AGGCCT 1 cut(s) 247
PfeI GAWTC 1 cut(s) 410
PkrI GCNGC 2 cut(s) 171, 174
PleI GAGTC 1 cut(s) 206
PpsI GAGTC 1 cut(s) 206
PpuMI RGGWCCY 1 cut(s) 102
PshBI ATTAAT 1 cut(s) 428
Psp5II RGGWCCY 1 cut(s) 102
Psp6I CCWGG 1 cut(s) 51
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 3 cut(s) 60, 104, 379
PspPI GGNCC 1 cut(s) 102
PspPPI RGGWCCY 1 cut(s) 102
PstI CTGCAG 1 cut(s) 174
PsuI RGATCY 1 cut(s) 377
RsaI GTAC 2 cut(s) 203, 297
RsaNI GTAC 2 cut(s) 202, 296
SaqAI TTAA 4 cut(s) 45, 336, 369, 428
SatI GCNGC 2 cut(s) 170, 173
Sau3AI GATC 1 cut(s) 377
Sau96I GGNCC 1 cut(s) 102
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 1 cut(s) 53
SetI ASST 7 cut(s) 9, 36, 54, 74, 104, 171, 301
SfaNI GCATC 2 cut(s) 133, 316
SfcI CTRYAG 1 cut(s) 170
SfuI TTCGAA 1 cut(s) 182
SinI GGWCC 1 cut(s) 102
Sse9I AATT 3 cut(s) 156, 184, 429
SseBI AGGCCT 1 cut(s) 247
SspI AATATT 2 cut(s) 254, 373
StuI AGGCCT 1 cut(s) 247
StyD4I CCNGG 1 cut(s) 51
TaaI ACNGT 1 cut(s) 21
TaqI TCGA 3 cut(s) 12, 182, 188
TasI AATT 3 cut(s) 156, 184, 429
TfiI GAWTC 1 cut(s) 410
Tru1I TTAA 4 cut(s) 45, 336, 369, 428
Tru9I TTAA 4 cut(s) 45, 336, 369, 428
TscAI CASTG 1 cut(s) 412
TseI GCWGC 2 cut(s) 169, 172
TspDTI ATGAA 1 cut(s) 408
TspGWI ACGGA 1 cut(s) 145
TspRI CASTG 1 cut(s) 412
VpaK11BI GGWCC 1 cut(s) 102
VspI ATTAAT 1 cut(s) 428
XapI RAATTY 1 cut(s) 184
Zsp2I ATGCAT 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.