FvH4_6g51490

Protein kinase PVPK-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007009
Physical Location & Seq
Forward (+)
38169097 .. 38172864
3768 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g51490.t3

Sequence Viewer

Length: 1791 bp
ATGGGGAAACGGTACCAGGGTTCGAGTAAAGGCAAAGTTGAACATGGAGGCACGGATGATGTTCTTGTTGATCCAGCCGAAAGCTCTTATCAGAGCAAGGTTTCTATGGTGGAAGCAAAACCTCCCGTCCAGCATCCTGTTGATGATTCTGAGGAATTTGGTTCAAGCGGCTTACCAGAATCTAGGAATTATGTTTTAGGTCCGGGTAAAGGAGTTGCCGGACGGAAAAATGGCAACTTAGTTTCTCATTCAGGAGTAGGGACTGCCTATTGTCCAAGCCCTCAGAACAGCTTATATTCTGCTGCTCCCACTCTCTATTGTGAAGCCAAACAGAGTTTTACCAATACTGAAGTTAGTGAATGTACAAGCAGCATTGAGAAGTCTGCTGAAACTGAAAGTGAGGTCACTAATTCCTGTGAGCTTATCGAGAGCAGAAAGACCAGTATGTATAGAGGCAGCACCGGAAGTGATGTTAGTGATGAGAGCAGCTCCAGTAGTTTGAGCAATAACATGTACAAGCCCCACAAGGCGAATGATGTGAGATGGGAAGCAATTCAAGCTGTCCGAGATCATGATGGGATGCTGGGTTTGAATCATTTCAGGTTGGTGAAGAGACTGGGATGTGGAGATATAGGCAGTGTTTATTTGTCTGAACTGTCAGGCACTAGAACTTGTTTTGCCATGAAGGTTATGGATAAAGCACAACTGGCAGGTCGCAAAAAGCTTTTGAGGGCTCAGACAGAAAGAGAGATACTACAGTCTCTGGATCACCCTTTCCTGCCCACATTGTACTCCCACTTTGAGACAGAGAAGTTCACATGCTTGTTGATGGAGTATTGCCCTGGAGGTGATCTGCATGCACTTAGGCAAAAACAACCTGGAAAGTATTTTCCGGAGCATGCTGCCAGGTTTTATGTGGCTGAAGTTCTGCTTGCTTTGGAGTATTTGCATATGCTTGGGATCATTTACAGAGACCTCAAACCAGAGAATGTTTTAGTGAGGGAAGATGGACATATAATGCTTTCAGATTTTGACCTTTCATTAAGATGTGCTGTTTCCCCCACCCTGGTTAGATCTTCAATCGCAAGCATGGAGACAAAGAAATCAGCATATTGTGTTCAGCCTGCATGCATCGAGCCAACTTGTGTAATGCAGCCAGATTGCATTACGCCAGCTTGCTTTGCTCCACGCTTTTTCTCAAGCAAGCCCAAGAAGGAGAAGAAGAAGACCAAACCAAAGAATGACATATATAATCAAGTGAGCCCTCTCCCTGAGCTTATTGCAGAACCAACAAGTGCTAGATCAATGTCCTTTGTGGGAACACACGAGTACCTGGCACCTGAAATCATTAAAGGTGAAGGCCATGGAAGTGCTGTGGATTGGTGGACATTTGGGATCTTTCTCTATGAGCTTTTATTTGGCAAAACTCCATTCAAGGGAGCAGGGAACCGAGCTACTTTGTTTAATGTGGTTGGGCAGCCTTTAAGATTTCCAGAGTCGCCTAGTGTCAGCTTTGCAGCGAGGGACTTGATCAGAGGTCTACTTGTGAAAGAGCCACAGCATCGTCTTGCTTATCGGCGTGGGGCTACAGAAGTGAAACAGCATCCGTTTTTCCAGAGTGTGAATTGGGCACTAATCCGGTGTACAAATCCGCCTTCGGTGCCACCAAAACAGAACCCGTTGGACATTTCTCCACGACCTGATACTCCGAAAGCAGCCAGCACAGACGGGAAGGTACCAGGTGTTGATTTGAAGCCTTCTGGTAATTATATAGAGATTGATTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

597

Amino Acids

65.84

Weight (kDa)

7.83

Isoelectric Point (pI)

47.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 290 - 440 8.4e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 290 - 439 4e-18 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 524 - 627 1.3e-20 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 531 - 614 3.5e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 701
Acc65I GGTACC 2 cut(s) 12, 1735
AccB1I GGYRCC 4 cut(s) 12, 1336, 1660, 1735
AccI GTMKAC 1 cut(s) 1540
AccIII TCCGGA 1 cut(s) 892
AciI CCGC 2 cut(s) 168, 1652
AclWI GGATC 4 cut(s) 65, 774, 968, 1403
AcsI RAATTY 1 cut(s) 155
AcuI CTGAAG 2 cut(s) 369, 942
AfaI GTAC 7 cut(s) 14, 364, 515, 791, 1331, 1645, 1737
AfiI CCNNNNNNNGG 3 cut(s) 209, 1066, 1436
AflIII ACRYGT 1 cut(s) 510
AgsI TTSAA 7 cut(s) 41, 165, 557, 592, 1080, 1435, 1753
AjnI CCWGG 7 cut(s) 15, 841, 877, 905, 1065, 1332, 1738
Alw26I GTCTC 5 cut(s) 607, 765, 797, 966, 1088
AlwI GGATC 4 cut(s) 65, 774, 968, 1403
AlwNI CAGNNNCTG 1 cut(s) 763
Aor13HI TCCGGA 1 cut(s) 892
AoxI GGCC 1 cut(s) 1360
ApeKI GCWGC 9 cut(s) 302, 369, 456, 486, 902, 1153, 1477, 1517, 1715
ApoI RAATTY 1 cut(s) 155
Asp700I GAANNNNTTC 2 cut(s) 552, 596
Asp718I GGTACC 2 cut(s) 12, 1735
AspS9I GGNCC 1 cut(s) 200
AsuC2I CCSGG 1 cut(s) 204
AsuHPI GGTGA 4 cut(s) 619, 761, 860, 1367
AvaII GGWCC 1 cut(s) 200
BaeGI GKGCMC 1 cut(s) 1633
BaeI ACNNNNGTAYC 5 cut(s) 37, 743, 776, 1313, 1346
BanI GGYRCC 4 cut(s) 12, 1336, 1660, 1735
BanII GRGCYC 2 cut(s) 736, 1265
BauI CACGAG 1 cut(s) 1325
BbsI GAAGAC 1 cut(s) 1232
BbvI GCAGC 9 cut(s) 289, 381, 468, 498, 889, 1165, 1489, 1529, 1727
BccI CCATC 4 cut(s) 537, 569, 823, 1001
BciT130I CCWGG 7 cut(s) 17, 843, 879, 907, 1067, 1334, 1740
BclI TGATCA 1 cut(s) 1530
BcnI CCSGG 1 cut(s) 204
BcoDI GTCTC 5 cut(s) 607, 765, 797, 966, 1088
BfaI CTAG 4 cut(s) 183, 666, 1299, 1503
BfmI CTRYAG 2 cut(s) 755, 1587
BfuAI ACCTGC 1 cut(s) 701
BglII AGATCT 1 cut(s) 1073
Bme1390I CCNGG 8 cut(s) 17, 204, 843, 879, 907, 1067, 1334, 1740
Bme18I GGWCC 1 cut(s) 200
BmgT120I GGNCC 1 cut(s) 200
BmiI GGNNCC 5 cut(s) 14, 1338, 1448, 1662, 1737
BmrFI CCNGG 8 cut(s) 17, 204, 843, 879, 907, 1067, 1334, 1740
BmrI ACTGGG 1 cut(s) 626
BmsI GCATC 5 cut(s) 142, 570, 1140, 1570, 1612
BmuI ACTGGG 1 cut(s) 626
BpiI GAAGAC 1 cut(s) 1232
BplI GAGNNNNNCTC 2 cut(s) 473, 505
BpmI CTGGAG 2 cut(s) 475, 864
Bpu10I CCTNAGC 1 cut(s) 1272
BpuEI CTTGAG 1 cut(s) 1183
BpuMI CCSGG 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 966
BsaJI CCNNGG 4 cut(s) 16, 841, 1065, 1363
BsaWI WCCGGW 3 cut(s) 461, 892, 1638
Bsc4I CCNNNNNNNGG 3 cut(s) 209, 1066, 1436
Bse1I ACTGG 4 cut(s) 441, 492, 621, 711
BseAI TCCGGA 1 cut(s) 892
BseBI CCWGG 7 cut(s) 17, 843, 879, 907, 1067, 1334, 1740
BseDI CCNNGG 4 cut(s) 16, 841, 1065, 1363
BseGI GGATG 5 cut(s) 61, 133, 585, 626, 1603
BseLI CCNNNNNNNGG 3 cut(s) 209, 1066, 1436
BseMII CTCAG 4 cut(s) 141, 296, 749, 1263
BseNI ACTGG 4 cut(s) 441, 492, 621, 711
BseSI GKGCMC 1 cut(s) 1633
BseXI GCAGC 9 cut(s) 289, 381, 468, 498, 889, 1165, 1489, 1529, 1727
BseYI CCCAGC 1 cut(s) 583
BshFI GGCC 1 cut(s) 1362
BshNI GGYRCC 4 cut(s) 12, 1336, 1660, 1735
BsiSI CCGG 5 cut(s) 203, 219, 462, 893, 1639
BslFI GGGAC 2 cut(s) 274, 1538
BslI CCNNNNNNNGG 3 cut(s) 209, 1066, 1436
BsmAI GTCTC 5 cut(s) 607, 765, 797, 966, 1088
BsmFI GGGAC 2 cut(s) 274, 1538
BsnI GGCC 1 cut(s) 1362
Bso31I GGTCTC 1 cut(s) 966
Bsp1286I GDGCHC 3 cut(s) 736, 1265, 1633
Bsp13I TCCGGA 1 cut(s) 892
Bsp1407I TGTACA 3 cut(s) 362, 513, 1643
Bsp143I GATC 9 cut(s) 70, 568, 766, 850, 960, 1073, 1301, 1395, 1530
Bsp19I CCATGG 1 cut(s) 1363
BspACI CCGC 2 cut(s) 168, 1652
BspANI GGCC 1 cut(s) 1362
BspCNI CTCAG 4 cut(s) 142, 295, 748, 1264
BspEI TCCGGA 1 cut(s) 892
BspHI TCATGA 1 cut(s) 571
BspLI GGNNCC 5 cut(s) 14, 1338, 1448, 1662, 1737
BspMI ACCTGC 1 cut(s) 701
BspPI GGATC 4 cut(s) 65, 774, 968, 1403
BspT107I GGYRCC 4 cut(s) 12, 1336, 1660, 1735
BspTNI GGTCTC 1 cut(s) 966
BsrGI TGTACA 3 cut(s) 362, 513, 1643
BsrI ACTGG 4 cut(s) 441, 492, 621, 711
BssECI CCNNGG 4 cut(s) 16, 841, 1065, 1363
BssMI GATC 9 cut(s) 70, 568, 766, 850, 960, 1073, 1301, 1395, 1530
BssSI CACGAG 1 cut(s) 1325
BssT1I CCWWGG 1 cut(s) 1363
Bst2BI CACGAG 1 cut(s) 1325
Bst2UI CCWGG 7 cut(s) 17, 843, 879, 907, 1067, 1334, 1740
Bst4CI ACNGT 3 cut(s) 12, 657, 759
Bst6I CTCTTC 1 cut(s) 605
BstAUI TGTACA 3 cut(s) 362, 513, 1643
BstDEI CTNAG 6 cut(s) 150, 238, 282, 735, 863, 1272
BstDSI CCRYGG 1 cut(s) 1363
BstF5I GGATG 5 cut(s) 61, 133, 585, 626, 1603
BstKTI GATC 9 cut(s) 73, 571, 769, 853, 963, 1076, 1304, 1398, 1533
BstMAI GTCTC 5 cut(s) 607, 765, 797, 966, 1088
BstMBI GATC 9 cut(s) 70, 568, 766, 850, 960, 1073, 1301, 1395, 1530
BstMWI GCNNNNNNNGC 4 cut(s) 557, 707, 1181, 1660
BstNI CCWGG 7 cut(s) 17, 843, 879, 907, 1067, 1334, 1740
BstNSI RCATGY 5 cut(s) 514, 822, 860, 902, 1131
BstSCI CCNGG 8 cut(s) 15, 202, 841, 877, 905, 1065, 1332, 1738
BstSFI CTRYAG 2 cut(s) 755, 1587
BstSLI GKGCMC 1 cut(s) 1633
BstV1I GCAGC 9 cut(s) 289, 381, 468, 498, 889, 1165, 1489, 1529, 1727
BstV2I GAAGAC 1 cut(s) 1232
BstX2I RGATCY 2 cut(s) 1073, 1395
BstYI RGATCY 2 cut(s) 1073, 1395
BsuRI GGCC 1 cut(s) 1362
BtgI CCRYGG 1 cut(s) 1363
BtsCI GGATG 5 cut(s) 61, 133, 585, 626, 1603
BtsI GCAGTG 1 cut(s) 643
BtsIMutI CAGTG 1 cut(s) 643
BveI ACCTGC 1 cut(s) 701
CaiI CAGNNNCTG 1 cut(s) 763
CciI TCATGA 1 cut(s) 571
Cfr13I GGNCC 1 cut(s) 200
CsiI ACCWGGT 1 cut(s) 1738
Csp6I GTAC 7 cut(s) 13, 363, 514, 790, 1330, 1644, 1736
CviQI GTAC 7 cut(s) 13, 363, 514, 790, 1330, 1644, 1736
DdeI CTNAG 6 cut(s) 150, 238, 282, 735, 863, 1272
DpnI GATC 9 cut(s) 72, 570, 768, 852, 962, 1075, 1303, 1397, 1532
DpnII GATC 9 cut(s) 70, 568, 766, 850, 960, 1073, 1301, 1395, 1530
Eam1104I CTCTTC 1 cut(s) 605
EarI CTCTTC 1 cut(s) 605
EciI GGCGGA 1 cut(s) 1641
Eco130I CCWWGG 1 cut(s) 1363
Eco24I GRGCYC 2 cut(s) 736, 1265
Eco31I GGTCTC 1 cut(s) 966
Eco47I GGWCC 1 cut(s) 200
Eco57I CTGAAG 2 cut(s) 369, 942
EcoRII CCWGG 7 cut(s) 15, 841, 877, 905, 1065, 1332, 1738
EcoT14I CCWWGG 1 cut(s) 1363
EcoT22I ATGCAT 1 cut(s) 1133
EcoT38I GRGCYC 2 cut(s) 736, 1265
ErhI CCWWGG 1 cut(s) 1363
FalI AAGNNNNNCTT 2 cut(s) 915, 947
FaqI GGGAC 2 cut(s) 274, 1538
FauNDI CATATG 1 cut(s) 951
FbaI TGATCA 1 cut(s) 1530
FblI GTMKAC 1 cut(s) 1540
FokI GGATG 5 cut(s) 68, 120, 592, 633, 1590
FriOI GRGCYC 2 cut(s) 736, 1265
FspBI CTAG 4 cut(s) 183, 666, 1299, 1503
GsaI CCCAGC 1 cut(s) 587
GsuI CTGGAG 2 cut(s) 475, 864
HaeIII GGCC 1 cut(s) 1362
HapII CCGG 5 cut(s) 203, 219, 462, 893, 1639
HindIII AAGCTT 1 cut(s) 722
HinfI GANTC 4 cut(s) 146, 179, 592, 1496
HpaII CCGG 5 cut(s) 203, 219, 462, 893, 1639
HphI GGTGA 4 cut(s) 619, 761, 860, 1367
Hpy166II GTNNAC 4 cut(s) 816, 1386, 1541, 1644
Hpy188I TCNGA 9 cut(s) 93, 151, 285, 566, 652, 738, 1027, 1535, 1710
Hpy188III TCNNGA 7 cut(s) 252, 427, 572, 764, 893, 1493, 1615
Hpy8I GTNNAC 4 cut(s) 816, 1386, 1541, 1644
HpyAV CCTTC 6 cut(s) 679, 1207, 1352, 1665, 1726, 1767
HpyCH4III ACNGT 3 cut(s) 12, 657, 759
HpyCH4V TGCA 9 cut(s) 856, 860, 949, 1127, 1131, 1153, 1164, 1283, 1517
HpyF10VI GCNNNNNNNGC 4 cut(s) 557, 707, 1181, 1660
HpyF3I CTNAG 6 cut(s) 150, 238, 282, 735, 863, 1272
Kpn2I TCCGGA 1 cut(s) 892
KpnI GGTACC 2 cut(s) 16, 1739
Ksp22I TGATCA 1 cut(s) 1530
Kzo9I GATC 9 cut(s) 70, 568, 766, 850, 960, 1073, 1301, 1395, 1530
LmnI GCTCC 5 cut(s) 310, 494, 895, 1189, 1439
Lsp1109I GCAGC 9 cut(s) 289, 381, 468, 498, 889, 1165, 1489, 1529, 1727
LweI GCATC 5 cut(s) 142, 570, 1140, 1570, 1612
MabI ACCWGGT 1 cut(s) 1738
MaeI CTAG 4 cut(s) 183, 666, 1299, 1503
MaeIII GTNAC 1 cut(s) 403
MalI GATC 9 cut(s) 72, 570, 768, 852, 962, 1075, 1303, 1397, 1532
MboI GATC 9 cut(s) 70, 568, 766, 850, 960, 1073, 1301, 1395, 1530
MboII GAAGA 6 cut(s) 622, 1016, 1068, 1231, 1234, 1237
MflI RGATCY 2 cut(s) 1073, 1395
MhlI GDGCHC 3 cut(s) 736, 1265, 1633
MluCI AATT 6 cut(s) 155, 187, 409, 552, 1624, 1765
MlyI GAGTC 1 cut(s) 1505
MmeI TCCRAC 1 cut(s) 1662
Mph1103I ATGCAT 1 cut(s) 1133
MroI TCCGGA 1 cut(s) 892
MroXI GAANNNNTTC 2 cut(s) 552, 596
MseI TTAA 4 cut(s) 1043, 1350, 1464, 1484
MslI CAYNNNNRTG 2 cut(s) 1045, 1368
MspI CCGG 5 cut(s) 203, 219, 462, 893, 1639
MspR9I CCNGG 8 cut(s) 17, 204, 843, 879, 907, 1067, 1334, 1740
MvaI CCWGG 7 cut(s) 17, 843, 879, 907, 1067, 1334, 1740
MwoI GCNNNNNNNGC 4 cut(s) 557, 707, 1181, 1660
NciI CCSGG 1 cut(s) 204
NcoI CCATGG 1 cut(s) 1363
NdeI CATATG 1 cut(s) 951
NdeII GATC 9 cut(s) 70, 568, 766, 850, 960, 1073, 1301, 1395, 1530
NlaIV GGNNCC 5 cut(s) 14, 1338, 1448, 1662, 1737
NmuCI GTSAC 1 cut(s) 403
NsiI ATGCAT 1 cut(s) 1133
NspI RCATGY 5 cut(s) 514, 822, 860, 902, 1131
PaeI GCATGC 3 cut(s) 860, 902, 1131
PagI TCATGA 1 cut(s) 571
PciI ACATGT 1 cut(s) 510
PdmI GAANNNNTTC 2 cut(s) 552, 596
PfeI GAWTC 3 cut(s) 146, 179, 592
PleI GAGTC 1 cut(s) 1504
PpsI GAGTC 1 cut(s) 1504
PscI ACATGT 1 cut(s) 510
Psp6I CCWGG 7 cut(s) 15, 841, 877, 905, 1065, 1332, 1738
PspFI CCCAGC 1 cut(s) 583
PspGI CCWGG 7 cut(s) 15, 841, 877, 905, 1065, 1332, 1738
PspN4I GGNNCC 5 cut(s) 14, 1338, 1448, 1662, 1737
PspPI GGNCC 1 cut(s) 200
PstNI CAGNNNCTG 1 cut(s) 763
PsuI RGATCY 2 cut(s) 1073, 1395
RsaI GTAC 7 cut(s) 14, 364, 515, 791, 1331, 1645, 1737
RsaNI GTAC 7 cut(s) 13, 363, 514, 790, 1330, 1644, 1736
RseI CAYNNNNRTG 2 cut(s) 1045, 1368
SaqAI TTAA 4 cut(s) 1043, 1350, 1464, 1484
Sau3AI GATC 9 cut(s) 70, 568, 766, 850, 960, 1073, 1301, 1395, 1530
Sau96I GGNCC 1 cut(s) 200
SchI GAGTC 1 cut(s) 1505
ScrFI CCNGG 8 cut(s) 17, 204, 843, 879, 907, 1067, 1334, 1740
SduI GDGCHC 3 cut(s) 736, 1265, 1633
SexAI ACCWGGT 1 cut(s) 1738
SfaNI GCATC 5 cut(s) 142, 570, 1140, 1570, 1612
SfcI CTRYAG 2 cut(s) 755, 1587
SinI GGWCC 1 cut(s) 200
SmiMI CAYNNNNRTG 2 cut(s) 1045, 1368
SmlI CTYRAG 1 cut(s) 1198
SmoI CTYRAG 1 cut(s) 1198
SphI GCATGC 3 cut(s) 860, 902, 1131
Sse9I AATT 6 cut(s) 155, 187, 409, 552, 1624, 1765
SsiI CCGC 2 cut(s) 168, 1652
SspMI CTAG 4 cut(s) 183, 666, 1299, 1503
StyD4I CCNGG 8 cut(s) 15, 202, 841, 877, 905, 1065, 1332, 1738
StyI CCWWGG 1 cut(s) 1363
TaaI ACNGT 3 cut(s) 12, 657, 759
TaqI TCGA 3 cut(s) 23, 426, 1134
TasI AATT 6 cut(s) 155, 187, 409, 552, 1624, 1765
TatI WGTACW 4 cut(s) 362, 513, 789, 1643
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 3 cut(s) 146, 179, 592
Tru1I TTAA 4 cut(s) 1043, 1350, 1464, 1484
Tru9I TTAA 4 cut(s) 1043, 1350, 1464, 1484
TscAI CASTG 1 cut(s) 643
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 9 cut(s) 302, 369, 456, 486, 902, 1153, 1477, 1517, 1715
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 2 cut(s) 698, 1029
TspGWI ACGGA 3 cut(s) 68, 238, 1596
TspRI CASTG 1 cut(s) 643
VpaK11BI GGWCC 1 cut(s) 200
XapI RAATTY 1 cut(s) 155
XceI RCATGY 5 cut(s) 514, 822, 860, 902, 1131
XcmI CCANNNNNNNNNTGG 2 cut(s) 688, 913
XmiI GTMKAC 1 cut(s) 1540
XmnI GAANNNNTTC 2 cut(s) 552, 596
XspI CTAG 4 cut(s) 183, 666, 1299, 1503
Zsp2I ATGCAT 1 cut(s) 1133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.