MD09G1026700.v1.1

Protein kinase PVPK-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007009
Physical Location & Seq
Reverse (-)
1659834 .. 1663287
3454 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1026700.v1.1.491

Sequence Viewer

Length: 2028 bp
ATGGGGTCACTGGTTGATGGGGTTAATTCTTTGCCGAAATTTCGCTATTCTGCACCGCCTGAGGTAGGCAAGAACCCTCCTTCCACAACTGGGACTCCTCGTCCTTCTCAGCCCTCATCTCCAAAACAATCAAGAAGCGAAGGGGTTTCGTCGCCATCCTGCGTGACTGCGCATACTTATTCGGTGAAGACAGACAGCTACTCAAATGGTTCTGTAAATAATCAAAAGAACCCCAACAAGGCAACTAATCATAACATGCAGCAGGAAGAGTTTCTTCATATGGGGAAACACTACCACAATTCGAATAAAGGAACACTTGAGCATGGAGGCCCAAATGATGTTCTTAACAAATCAGCCAAAACTTCTTATCTGAACAAGGTTTCCGTGCTGGAAGCAAAATCAAGCGTCAAGCATCCGGTTAATGATTGTGATTCGAGTAGATATATGGAATCTAGAAATCACGGTTTGGTTCCTTATAAAGGAGTAGCCGGGCAGACAAATAGCCACCTCATTTCTCACTGTGCAAGCCCTCAGAACAGTTTATATTCTGCCTCTCTATATTGTGAAGCCAAGCAGAGTTTCACCAATACAGAAGTCAGTGAATGTATCAACAGTGTGGAGAAGTCTGTTGAAAGCAGTGAAGTTACTAATTCCCGTGATCTTATTGAGAGCAGGACGACCAGCATCTATAGAGGCAGCACTGGCAGCGATGTTAGCGATGAGAGCAGCTCCAGTAGTTTGAGCAATGCCATGTACAAGCCCCACAAGGCAAATAATATTAGTTGGGAAGCGGTGCAAGCTGCACGATCTCATCATGATGGAACACTGGGTAAGGAGCATTTCAAGTTGCTGAGGACACTGGGATGTGGAGATATAGGCAGCGTTTATTTGGCTGAACTAATTGGCACTAAAACTTATTTCGCCATGAAGGTTATGGATAAAGCATTACTGGCAAGTAGGAAAAAACTTCTTAGGGCTCAGACAGAGAGGGAAATACTGCAATCTCTGGATCATCCTTTCCTGCCAACATTGTATACACACTTTGAGACGGAGAAGGTATCTTGTTTGGTGATGGAGTTTTGTCCCGGGGGTGATCTGCATGCACTCAGACAAAAACAACCTGGGATTGGGAAGCATTTTTCGGAGCATGCTGCTAGGTTTTATGTGTCTGAAGTTCTTCTTGCCTTGGAATATTTGCATATGCTCGGGATCATTTACAGAGACCTTAAACCAGAGAATGTTTTAGTGAGGGAAGATGGACATATAATGCTTTCAGATTTCGACCTTTCTTTAAGATGCACCGTTTCCCCCACCCTGGTAAGATCTTCAAACTTAAGCTTGGAGACAAAGAAATCAGCATATTGTGTTCAGCCTGCATGCATCCAGCCGACTTGTGTAATGCAGCCGGATTGCATTACGCCCACATGTTTTGGTCCACGCTTTTTCAAGGGCAAACCCAAGAAAGATAAAAAGATCAAAGTGAAGAATGATAATGATGTATACAATCAAGTGAGCCCTCTCCCTGAGCTCGTTGCAGAACCGACAAGTGCTAGATCAATGTCCTTTGTGGGAACACACGAGTACTTGGCGCCTGAAATCATTAAAGGTGAAGGCCATGGCAGCGCTGTGGATTGGTGGACCTTTGGGATCTTTCTCTACGAGCTTTTGTTCGGAAGAACTCCATTCAAGGGGCACGGAAATCGGGCTACTTTGTTTAATGTGGTTGGCCAGCCTTTAAGATTTCCAGAGTCACCTTCTGTCAGCTTTGCAGCCAGGGACTTGATCAGAGGTTTACTTGTCAAGGAGCCACAGCATCGTCTTGCGTATAGGCGGGGAGCAACAGAAGTTAAACAACATCCGTTTTTCCAAAGCGTGAATTGGGCACTGATTCGCTGTACAACTCCACCCCAGGTGCCAAAGCACTACATATTGGAGACTCCTGATACCCCGAAAGAACCCATGAACGGGAAGATGCCGGGCGTTGATTTGAAGCCTTCGGGTAATTATTTAGAGATTGATTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

676

Amino Acids

74.91

Weight (kDa)

8.74

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 282 - 434 1.4e-35 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 282 - 434 3.8e-19 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 519 - 622 1e-20 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 526 - 609 5.2e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 477
Acc16I TGCGCA 1 cut(s) 171
AccB1I GGYRCC 2 cut(s) 1588, 1912
AccI GTMKAC 2 cut(s) 1034, 1500
AciI CCGC 3 cut(s) 56, 791, 1831
AclWI GGATC 3 cut(s) 1017, 1217, 1655
AcoI YGGCCR 1 cut(s) 1726
AcsI RAATTY 1 cut(s) 38
AcuI CTGAAG 1 cut(s) 1191
AcyI GRCGYC 1 cut(s) 1589
AfaI GTAC 3 cut(s) 755, 1583, 1897
AfeI AGCGCT 1 cut(s) 1624
AfiI CCNNNNNNNGG 9 cut(s) 65, 90, 238, 479, 1127, 1315, 1688, 1964, 1965
AflII CTTAAG 1 cut(s) 1333
AflIII ACRYGT 1 cut(s) 1424
AgsI TTSAA 6 cut(s) 632, 844, 1329, 1447, 1687, 1990
AhdI GACNNNNNGTC 1 cut(s) 99
AjnI CCWGG 4 cut(s) 1120, 1314, 1772, 1908
AjuI GAANNNNNNNTTGG 2 cut(s) 563, 595
AloI GAACNNNNNNTCC 4 cut(s) 65, 97, 365, 397
AluBI AGCT 7 cut(s) 198, 729, 800, 1338, 1528, 1663, 1764
AluI AGCT 7 cut(s) 198, 729, 800, 1338, 1528, 1663, 1764
Alw21I GWGCWC 1 cut(s) 1530
Alw26I GTCTC 4 cut(s) 1040, 1215, 1337, 1928
AlwI GGATC 3 cut(s) 1017, 1217, 1655
Ama87I CYCGRG 2 cut(s) 1085, 1205
Aor51HI AGCGCT 1 cut(s) 1624
AoxI GGCC 3 cut(s) 328, 1612, 1726
ApoI RAATTY 1 cut(s) 38
Asp700I GAANNNNTTC 2 cut(s) 270, 1176
AspLEI GCGC 3 cut(s) 172, 1591, 1625
AspS9I GGNCC 3 cut(s) 329, 1433, 1638
AsuC2I CCSGG 4 cut(s) 490, 1086, 1087, 1977
AsuHPI GGTGA 6 cut(s) 196, 574, 1081, 1103, 1619, 1743
AsuII TTCGAA 1 cut(s) 302
AvaI CYCGRG 2 cut(s) 1085, 1205
AvaII GGWCC 2 cut(s) 1433, 1638
AxyI CCTNAGG 1 cut(s) 60
BaeGI GKGCMC 2 cut(s) 1695, 1885
BalI TGGCCA 1 cut(s) 1728
BanI GGYRCC 2 cut(s) 1588, 1912
BanII GRGCYC 3 cut(s) 979, 1517, 1530
BauI CACGAG 1 cut(s) 1577
BbsI GAAGAC 1 cut(s) 194
Bbv12I GWGCWC 1 cut(s) 1530
BbvCI CCTCAGC 1 cut(s) 851
BccI CCATC 5 cut(s) 11, 163, 812, 1066, 1250
BcgI CGANNNNNNTGC 2 cut(s) 1682, 1716
BciT130I CCWGG 4 cut(s) 1122, 1316, 1774, 1910
BclI TGATCA 1 cut(s) 1782
BcnI CCSGG 4 cut(s) 490, 1086, 1087, 1977
BcoDI GTCTC 4 cut(s) 1040, 1215, 1337, 1928
BfaI CTAG 3 cut(s) 453, 1155, 1551
BfmI CTRYAG 1 cut(s) 688
BfoI RGCGCY 2 cut(s) 1592, 1626
BfrI CTTAAG 1 cut(s) 1333
BglII AGATCT 1 cut(s) 1322
BmcAI AGTACT 1 cut(s) 1583
Bme1390I CCNGG 8 cut(s) 490, 1086, 1087, 1122, 1316, 1774, 1910, 1977
Bme18I GGWCC 2 cut(s) 1433, 1638
BmeRI GACNNNNNGTC 1 cut(s) 99
BmeT110I CYCGRG 2 cut(s) 1085, 1205
BmgT120I GGNCC 3 cut(s) 329, 1433, 1638
BmiI GGNNCC 4 cut(s) 471, 1590, 1806, 1914
BmrFI CCNGG 8 cut(s) 490, 1086, 1087, 1122, 1316, 1774, 1910, 1977
BmrI ACTGGG 3 cut(s) 99, 836, 869
BmsI GCATC 6 cut(s) 421, 693, 1286, 1389, 1822, 1962
BmuI ACTGGG 3 cut(s) 99, 836, 869
BpiI GAAGAC 1 cut(s) 194
BplI GAGNNNNNCTC 2 cut(s) 713, 745
BpmI CTGGAG 1 cut(s) 715
Bpu10I CCTNAGC 2 cut(s) 851, 1524
Bpu14I TTCGAA 1 cut(s) 302
BpuEI CTTGAG 1 cut(s) 338
BpuMI CCSGG 4 cut(s) 490, 1086, 1087, 1977
BsaHI GRCGYC 1 cut(s) 1589
BsaI GGTCTC 1 cut(s) 1215
BsaJI CCNNGG 8 cut(s) 1085, 1086, 1121, 1185, 1314, 1615, 1773, 1908
BsaWI WCCGGW 1 cut(s) 415
BsaXI ACNNNNNCTCC 2 cut(s) 79, 109
Bsc4I CCNNNNNNNGG 9 cut(s) 65, 90, 238, 479, 1127, 1315, 1688, 1964, 1965
Bse1I ACTGG 7 cut(s) 15, 94, 706, 732, 831, 864, 954
Bse21I CCTNAGG 1 cut(s) 60
Bse3DI GCAATG 1 cut(s) 751
BseBI CCWGG 4 cut(s) 1122, 1316, 1774, 1910
BseDI CCNNGG 8 cut(s) 1085, 1086, 1121, 1185, 1314, 1615, 1773, 1908
BseGI GGATG 6 cut(s) 155, 412, 869, 1012, 1380, 1855
BseLI CCNNNNNNNGG 9 cut(s) 65, 90, 238, 479, 1127, 1315, 1688, 1964, 1965
BseMI GCAATG 1 cut(s) 751
BseMII CTCAG 7 cut(s) 51, 122, 545, 842, 992, 1120, 1515
BseNI ACTGG 7 cut(s) 15, 94, 706, 732, 831, 864, 954
BseRI GAGGAG 1 cut(s) 87
BseSI GKGCMC 2 cut(s) 1695, 1885
BsgI GTGCAG 2 cut(s) 36, 786
BshFI GGCC 3 cut(s) 330, 1614, 1728
BshNI GGYRCC 2 cut(s) 1588, 1912
BsiHKAI GWGCWC 1 cut(s) 1530
BsiHKCI CYCGRG 2 cut(s) 1085, 1205
BsiSI CCGG 5 cut(s) 416, 489, 1086, 1406, 1976
BslFI GGGAC 3 cut(s) 106, 1068, 1790
BslI CCNNNNNNNGG 9 cut(s) 65, 90, 238, 479, 1127, 1315, 1688, 1964, 1965
BsmAI GTCTC 4 cut(s) 1040, 1215, 1337, 1928
BsmBI CGTCTC 1 cut(s) 1040
BsmFI GGGAC 3 cut(s) 106, 1068, 1790
BsnI GGCC 3 cut(s) 330, 1614, 1728
Bso31I GGTCTC 1 cut(s) 1215
BsoBI CYCGRG 2 cut(s) 1085, 1205
Bsp119I TTCGAA 1 cut(s) 302
Bsp1286I GDGCHC 5 cut(s) 979, 1517, 1530, 1695, 1885
Bsp1407I TGTACA 2 cut(s) 753, 1895
Bsp19I CCATGG 1 cut(s) 1615
BspACI CCGC 3 cut(s) 56, 791, 1831
BspANI GGCC 3 cut(s) 330, 1614, 1728
BspCNI CTCAG 7 cut(s) 52, 121, 544, 843, 991, 1119, 1516
BspHI TCATGA 1 cut(s) 814
BspLI GGNNCC 4 cut(s) 471, 1590, 1806, 1914
BspPI GGATC 3 cut(s) 1017, 1217, 1655
BspT104I TTCGAA 1 cut(s) 302
BspT107I GGYRCC 2 cut(s) 1588, 1912
BspTI CTTAAG 1 cut(s) 1333
BspTNI GGTCTC 1 cut(s) 1215
BsrDI GCAATG 1 cut(s) 751
BsrGI TGTACA 2 cut(s) 753, 1895
BsrI ACTGG 7 cut(s) 15, 94, 706, 732, 831, 864, 954
BssECI CCNNGG 8 cut(s) 1085, 1086, 1121, 1185, 1314, 1615, 1773, 1908
BssNAI GTATAC 2 cut(s) 1035, 1501
BssNI GRCGYC 1 cut(s) 1589
BssSI CACGAG 1 cut(s) 1577
BssT1I CCWWGG 2 cut(s) 1185, 1615
Bst1107I GTATAC 2 cut(s) 1035, 1501
Bst2BI CACGAG 1 cut(s) 1577
Bst2UI CCWGG 4 cut(s) 1122, 1316, 1774, 1910
Bst4CI ACNGT 5 cut(s) 464, 521, 539, 614, 1303
Bst6I CTCTTC 1 cut(s) 261
BstACI GRCGYC 1 cut(s) 1589
BstAFI CTTAAG 1 cut(s) 1333
BstAUI TGTACA 2 cut(s) 753, 1895
BstBI TTCGAA 1 cut(s) 302
BstC8I GCNNGC 7 cut(s) 526, 798, 1101, 1149, 1374, 1378, 1730
BstDEI CTNAG 8 cut(s) 60, 108, 531, 851, 971, 978, 1106, 1524
BstDSI CCRYGG 1 cut(s) 1615
BstENI CCTNNNNNAGG 2 cut(s) 63, 477
BstF5I GGATG 6 cut(s) 155, 412, 869, 1012, 1380, 1855
BstH2I RGCGCY 2 cut(s) 1592, 1626
BstHHI GCGC 3 cut(s) 172, 1591, 1625
BstMAI GTCTC 4 cut(s) 1040, 1215, 1337, 1928
BstMWI GCNNNNNNNGC 7 cut(s) 702, 705, 714, 723, 797, 950, 1620
BstNI CCWGG 4 cut(s) 1122, 1316, 1774, 1910
BstNSI RCATGY 5 cut(s) 259, 1103, 1151, 1380, 1428
BstSCI CCNGG 8 cut(s) 488, 1084, 1085, 1120, 1314, 1772, 1908, 1975
BstSFI CTRYAG 1 cut(s) 688
BstSLI GKGCMC 2 cut(s) 1695, 1885
BstV2I GAAGAC 1 cut(s) 194
BstX2I RGATCY 2 cut(s) 1322, 1647
BstYI RGATCY 2 cut(s) 1322, 1647
BstZ17I GTATAC 2 cut(s) 1035, 1501
Bsu36I CCTNAGG 1 cut(s) 60
BsuRI GGCC 3 cut(s) 330, 1614, 1728
BtgI CCRYGG 1 cut(s) 1615
BtgZI GCGATG 2 cut(s) 723, 732
BtsCI GGATG 6 cut(s) 155, 412, 869, 1012, 1380, 1855
BtsI GCAGTG 1 cut(s) 643
BtsIMutI CAGTG 9 cut(s) 8, 517, 604, 619, 643, 699, 824, 857, 1883
Cac8I GCNNGC 7 cut(s) 526, 798, 1101, 1149, 1374, 1378, 1730
CciI TCATGA 1 cut(s) 814
CfoI GCGC 3 cut(s) 172, 1591, 1625
Cfr13I GGNCC 3 cut(s) 329, 1433, 1638
Cfr9I CCCGGG 1 cut(s) 1085
CseI GACGC 1 cut(s) 394
Csp6I GTAC 3 cut(s) 754, 1582, 1896
CspCI CAANNNNNGTGG 2 cut(s) 1411, 1446
CviQI GTAC 3 cut(s) 754, 1582, 1896
DdeI CTNAG 8 cut(s) 60, 108, 531, 851, 971, 978, 1106, 1524
DinI GGCGCC 1 cut(s) 1590
DriI GACNNNNNGTC 1 cut(s) 99
EaeI YGGCCR 1 cut(s) 1726
Eam1104I CTCTTC 1 cut(s) 261
Eam1105I GACNNNNNGTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 261
Ecl136II GAGCTC 1 cut(s) 1528
Eco130I CCWWGG 2 cut(s) 1185, 1615
Eco24I GRGCYC 3 cut(s) 979, 1517, 1530
Eco31I GGTCTC 1 cut(s) 1215
Eco47I GGWCC 2 cut(s) 1433, 1638
Eco47III AGCGCT 1 cut(s) 1624
Eco53kI GAGCTC 1 cut(s) 1528
Eco57I CTGAAG 1 cut(s) 1191
Eco81I CCTNAGG 1 cut(s) 60
Eco88I CYCGRG 2 cut(s) 1085, 1205
EcoICRI GAGCTC 1 cut(s) 1528
EcoNI CCTNNNNNAGG 2 cut(s) 63, 477
EcoRII CCWGG 4 cut(s) 1120, 1314, 1772, 1908
EcoT14I CCWWGG 2 cut(s) 1185, 1615
EcoT22I ATGCAT 1 cut(s) 1382
EcoT38I GRGCYC 3 cut(s) 979, 1517, 1530
EgeI GGCGCC 1 cut(s) 1590
EheI GGCGCC 1 cut(s) 1590
ErhI CCWWGG 2 cut(s) 1185, 1615
Esp3I CGTCTC 1 cut(s) 1040
FalI AAGNNNNNCTT 6 cut(s) 258, 290, 300, 332, 1164, 1196
FaqI GGGAC 3 cut(s) 106, 1068, 1790
FauI CCCGC 1 cut(s) 1824
FauNDI CATATG 2 cut(s) 279, 1200
FbaI TGATCA 1 cut(s) 1782
FblI GTMKAC 2 cut(s) 1034, 1500
FokI GGATG 6 cut(s) 142, 399, 876, 999, 1367, 1842
FriOI GRGCYC 3 cut(s) 979, 1517, 1530
FspBI CTAG 3 cut(s) 453, 1155, 1551
FspI TGCGCA 1 cut(s) 171
GlaI GCGC 3 cut(s) 171, 1590, 1624
GsuI CTGGAG 1 cut(s) 715
HaeII RGCGCY 2 cut(s) 1592, 1626
HaeIII GGCC 3 cut(s) 330, 1614, 1728
HapII CCGG 5 cut(s) 416, 489, 1086, 1406, 1976
HgaI GACGC 1 cut(s) 394
HhaI GCGC 3 cut(s) 172, 1591, 1625
Hin1I GRCGYC 1 cut(s) 1589
Hin6I GCGC 3 cut(s) 170, 1589, 1623
HinP1I GCGC 3 cut(s) 170, 1589, 1623
HindIII AAGCTT 1 cut(s) 1336
HinfI GANTC 6 cut(s) 94, 431, 449, 1748, 1888, 1936
HpaII CCGG 5 cut(s) 416, 489, 1086, 1406, 1976
HphI GGTGA 6 cut(s) 196, 574, 1081, 1103, 1619, 1743
Hpy166II GTNNAC 5 cut(s) 1035, 1436, 1501, 1638, 1793
Hpy188I TCNGA 9 cut(s) 372, 534, 981, 1109, 1144, 1171, 1276, 1673, 1787
Hpy188III TCNNGA 7 cut(s) 132, 453, 815, 1007, 1207, 1745, 1940
Hpy8I GTNNAC 5 cut(s) 1035, 1436, 1501, 1638, 1793
Hpy99I CGWCG 1 cut(s) 154
HpyAV CCTTC 8 cut(s) 90, 114, 134, 922, 1048, 1604, 1764, 2004
HpyCH4III ACNGT 5 cut(s) 464, 521, 539, 614, 1303
HpyF10VI GCNNNNNNNGC 7 cut(s) 702, 705, 714, 723, 797, 950, 1620
HpyF3I CTNAG 8 cut(s) 60, 108, 531, 851, 971, 978, 1106, 1524
Hsp92I GRCGYC 1 cut(s) 1589
HspAI GCGC 3 cut(s) 170, 1589, 1623
KasI GGCGCC 1 cut(s) 1588
Ksp22I TGATCA 1 cut(s) 1782
LmnI GCTCC 5 cut(s) 734, 835, 1144, 1804, 1835
LweI GCATC 6 cut(s) 421, 693, 1286, 1389, 1822, 1962
MaeI CTAG 3 cut(s) 453, 1155, 1551
MaeIII GTNAC 4 cut(s) 6, 163, 643, 1749
MboII GAAGA 9 cut(s) 199, 266, 278, 1169, 1265, 1317, 1495, 1686, 1981
MflI RGATCY 2 cut(s) 1322, 1647
MhlI GDGCHC 5 cut(s) 979, 1517, 1530, 1695, 1885
MlsI TGGCCA 1 cut(s) 1728
MluCI AATT 7 cut(s) 25, 38, 298, 649, 900, 1876, 2002
MluNI TGGCCA 1 cut(s) 1728
Mly113I GGCGCC 1 cut(s) 1589
MlyI GAGTC 3 cut(s) 88, 1757, 1930
Mox20I TGGCCA 1 cut(s) 1728
Mph1103I ATGCAT 1 cut(s) 1382
MroXI GAANNNNTTC 2 cut(s) 270, 1176
MscI TGGCCA 1 cut(s) 1728
MslI CAYNNNNRTG 2 cut(s) 816, 862
Msp20I TGGCCA 1 cut(s) 1728
MspCI CTTAAG 1 cut(s) 1333
MspI CCGG 5 cut(s) 416, 489, 1086, 1406, 1976
MspR9I CCNGG 8 cut(s) 490, 1086, 1087, 1122, 1316, 1774, 1910, 1977
MvaI CCWGG 4 cut(s) 1122, 1316, 1774, 1910
MwoI GCNNNNNNNGC 7 cut(s) 702, 705, 714, 723, 797, 950, 1620
NarI GGCGCC 1 cut(s) 1589
NciI CCSGG 4 cut(s) 490, 1086, 1087, 1977
NcoI CCATGG 1 cut(s) 1615
NdeI CATATG 2 cut(s) 279, 1200
NlaIV GGNNCC 4 cut(s) 471, 1590, 1806, 1914
NmuCI GTSAC 3 cut(s) 6, 163, 1749
NsbI TGCGCA 1 cut(s) 171
NsiI ATGCAT 1 cut(s) 1382
NspI RCATGY 5 cut(s) 259, 1103, 1151, 1380, 1428
NspV TTCGAA 1 cut(s) 302
PaeI GCATGC 3 cut(s) 1103, 1151, 1380
PagI TCATGA 1 cut(s) 814
PciI ACATGT 1 cut(s) 1424
PdmI GAANNNNTTC 2 cut(s) 270, 1176
PfeI GAWTC 3 cut(s) 431, 449, 1888
PleI GAGTC 3 cut(s) 88, 1756, 1930
PluTI GGCGCC 1 cut(s) 1592
PpsI GAGTC 3 cut(s) 88, 1756, 1930
PscI ACATGT 1 cut(s) 1424
PsiI TTATAA 1 cut(s) 477
Psp124BI GAGCTC 1 cut(s) 1530
Psp6I CCWGG 4 cut(s) 1120, 1314, 1772, 1908
PspGI CCWGG 4 cut(s) 1120, 1314, 1772, 1908
PspN4I GGNNCC 4 cut(s) 471, 1590, 1806, 1914
PspPI GGNCC 3 cut(s) 329, 1433, 1638
PsuI RGATCY 2 cut(s) 1322, 1647
RsaI GTAC 3 cut(s) 755, 1583, 1897
RsaNI GTAC 3 cut(s) 754, 1582, 1896
RseI CAYNNNNRTG 2 cut(s) 816, 862
SacI GAGCTC 1 cut(s) 1530
Sau96I GGNCC 3 cut(s) 329, 1433, 1638
ScaI AGTACT 1 cut(s) 1583
SchI GAGTC 3 cut(s) 88, 1757, 1930
ScrFI CCNGG 8 cut(s) 490, 1086, 1087, 1122, 1316, 1774, 1910, 1977
SduI GDGCHC 5 cut(s) 979, 1517, 1530, 1695, 1885
SfaNI GCATC 6 cut(s) 421, 693, 1286, 1389, 1822, 1962
SfcI CTRYAG 1 cut(s) 688
SfoI GGCGCC 1 cut(s) 1590
SfuI TTCGAA 1 cut(s) 302
SinI GGWCC 2 cut(s) 1433, 1638
SmaI CCCGGG 1 cut(s) 1087
SmiMI CAYNNNNRTG 2 cut(s) 816, 862
SmlI CTYRAG 2 cut(s) 317, 1333
SmoI CTYRAG 2 cut(s) 317, 1333
SphI GCATGC 3 cut(s) 1103, 1151, 1380
Sse9I AATT 7 cut(s) 25, 38, 298, 649, 900, 1876, 2002
SsiI CCGC 3 cut(s) 56, 791, 1831
SspDI GGCGCC 1 cut(s) 1588
SspI AATATT 2 cut(s) 778, 1193
SspMI CTAG 3 cut(s) 453, 1155, 1551
SstI GAGCTC 1 cut(s) 1530
StyD4I CCNGG 8 cut(s) 488, 1084, 1085, 1120, 1314, 1772, 1908, 1975
StyI CCWWGG 2 cut(s) 1185, 1615
TaaI ACNGT 5 cut(s) 464, 521, 539, 614, 1303
TaqI TCGA 3 cut(s) 302, 434, 1281
TasI AATT 7 cut(s) 25, 38, 298, 649, 900, 1876, 2002
TatI WGTACW 3 cut(s) 753, 1581, 1895
TfiI GAWTC 3 cut(s) 431, 449, 1888
TscAI CASTG 9 cut(s) 15, 524, 604, 619, 643, 706, 831, 864, 1890
TseFI GTSAC 3 cut(s) 6, 163, 1749
Tsp45I GTSAC 3 cut(s) 6, 163, 1749
TspDTI ATGAA 3 cut(s) 266, 941, 1976
TspGWI ACGGA 4 cut(s) 373, 1064, 1710, 1848
TspMI CCCGGG 1 cut(s) 1085
TspRI CASTG 9 cut(s) 15, 524, 604, 619, 643, 706, 831, 864, 1890
Vha464I CTTAAG 1 cut(s) 1333
VpaK11BI GGWCC 2 cut(s) 1433, 1638
XagI CCTNNNNNAGG 2 cut(s) 63, 477
XapI RAATTY 1 cut(s) 38
XbaI TCTAGA 1 cut(s) 452
XceI RCATGY 5 cut(s) 259, 1103, 1151, 1380, 1428
XcmI CCANNNNNNNNNTGG 1 cut(s) 931
XmaI CCCGGG 1 cut(s) 1085
XmiI GTMKAC 2 cut(s) 1034, 1500
XmnI GAANNNNTTC 2 cut(s) 270, 1176
XspI CTAG 3 cut(s) 453, 1155, 1551
ZrmI AGTACT 1 cut(s) 1583
Zsp2I ATGCAT 1 cut(s) 1382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.