pycom111g02080

Protein kinase PVPK-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0007009
Physical Location & Seq
Reverse (-)
1247158 .. 1249379
2222 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g02080.1

Sequence Viewer

Length: 2025 bp
ATGGGGTCACTGGTTGATGGGGTTAATTCTTTGCCCAAAATTCGCTATTCTGCACCGCCTGAGGTAGGCAAGAACCTTCCTTCCACAACTGGGACTCCTCGTCCTTCTCAGCCTTCATCTCCAAAACAATCAAGAAGCGAAGGGGTTTCGTCGCCATCCTGCATGACTGCGCATACTTATTCGGTGAAGACAGACAGCTACTCAAATAGTTCCGTAAATAATCAAAAGAACCCCAACAAGACAACTAATCATAGCATGCAGCAGGAAGAGTTTCTTCATATGGGGAAACACTACCACAATTCGAATAAAGGAACACTCGAACATGGAGGCCCAAATGATGTTCTTAACAAATCTGCCGAAACTTCTTATCTGAACAAGGTTTCCGTGCTGGAAGCAAAATCAAGTTTCAAGCATCCAGTTAATGATTGTGATTCGAGTAGATATCTGGAATCTAGTAATCACAGTTTGGTTCCTTATAAAGGAGTAGCCGGGCAGACAAATAGCCACCTCATTTCTCACTGTGCAAGCCCTCAGAACAGTTTATATTCTGCCTCTCTGTATTGTGAAGCCAAGCAGAGTTTCACCAATACAGAAGTCAGTGAATGTATCAGCAGTGTGGAGAAGTCTGTTGAAAGCAGCGAAGTTACTAATTCCTGTGATCTTATTGAGAGCAGGACGACCAGCATCTATAGAGGCAGCACTGGCAGCGATGTTAGCGATGAGAGCAGCTCCAGTAGTTTGAGCAATGCCATGTACAAGCCCCACAAGGCAAATGATATTAGTTGGGAAGCGGTGCAAGCTGCGCGATCTCATCATGATGGAACACTGGGTAAGGAGCATTTCAAGTTGCTGAGGACACTGGGATGTGGAGATATAGGCAGCGTTTATTTGGCTGAACTGATTGGCACTAAAACTTATTTTGCCATGAAGGTTATGGATAAAGCATTACTGGCAAGTAGGAAAAAACTTCTTAGGGCTCAGACAGAGAGGGAAATACTGCAATCTCTGGATCATCCTTTCCTGCCAACATTGTATACACACTTTGAGACGGCAAAGGTATCTTGTTTGGTGATGGAGTTTTGTCCCGGGGGTGACCTGCATGCACTCAGACAAAAACAACCTGGGAAGCATTTTTCGGAGCATGCTGCTAGGTTTTATGTGGCTGAAGTTCTTCTAGCCTTGGAATATTTGCATATGCTCGGGATCATTTACAGAGACCTCAAACCAGAGAATGTTTTGGTGAGGGAAGATGGACATATAATGCTTTCAGATTTCGACCTTTCTTTAAGATGTGCCGTTTCCCCCACCCTGGTAAGATCTTCAAACTTAAGTTTGGAGACAAAGAAATCAGCATATTGTGTTCAGCCTGCATGCATCCAGCCAACTTGTGTAATGCAGCCGGATTGCATTACGCCCACATGTTTTGGTCCACGCTTTTTCAAGGGCAAACCCAAGAAAGATAAAAAGATCAAAGTGAAGAAGAATGATAATGATGTATACAATCAAGTGAGCCCTCTCCCTGAACTCGTTGCAGAACCGACAAGTGCTAGATCAATGTCCTTTGTGGGAACACACGAGTACTTGGCGCCTGAAATCATTAAAGGTGAAGGCCATGGCAGCGCTGTAGATTGGTGGACCTTTGGGATCTTTCTCTACGAGCTTTTGTTCGGAAGAACTCCATTCAAGGGGCAAGGAAACCGGGCTACTTTGTTTAATGTGGTTGGCCAGCCTTTGAGATTTCCAGAGCTGCCTTCTGTCAGCTTTGCAGCCAGGGACTTGATCAGAGGTTTACTTGTCAAGGAGCCACAGCATCGTCTTGCGTATAGGCGGGGAGCAACAGAAGTTAAACAACATCCGTTTTTCCAAAGCGTGAATTGGGCACTAATTCGTTGTACAACTCCACCCCAGGTGCCAAAGCACTACATATTGGAGACTCCTGACACACCGAAAGAACCATTAAACGGGAAGATGCCGGGCGTTGATTTGAAGCCTTCGGGTAATTATTTAGAGATTGATTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

675

Amino Acids

74.72

Weight (kDa)

8.51

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 282 - 431 7e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 282 - 431 2.8e-19 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 518 - 621 9.5e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 525 - 607 9.6e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 477
Acc16I TGCGCA 1 cut(s) 171
Acc36I ACCTGC 1 cut(s) 1104
AccB1I GGYRCC 2 cut(s) 1585, 1909
AccI GTMKAC 2 cut(s) 1034, 1497
AccII CGCG 1 cut(s) 805
AciI CCGC 3 cut(s) 56, 791, 1828
AclWI GGATC 3 cut(s) 1017, 1211, 1652
AcoI YGGCCR 1 cut(s) 1723
AcsI RAATTY 1 cut(s) 39
AcuI CTGAAG 1 cut(s) 1185
AcyI GRCGYC 1 cut(s) 1586
AfaI GTAC 3 cut(s) 755, 1580, 1894
AfeI AGCGCT 1 cut(s) 1621
AfiI CCNNNNNNNGG 6 cut(s) 65, 90, 479, 1309, 1685, 1961
AflII CTTAAG 1 cut(s) 1327
AflIII ACRYGT 1 cut(s) 1418
AgsI TTSAA 7 cut(s) 409, 632, 844, 1323, 1441, 1684, 1987
AhdI GACNNNNNGTC 1 cut(s) 99
AjnI CCWGG 4 cut(s) 1120, 1308, 1769, 1905
AjuI GAANNNNNNNTTGG 2 cut(s) 563, 595
AloI GAACNNNNNNTCC 4 cut(s) 65, 97, 365, 397
AluBI AGCT 6 cut(s) 198, 729, 800, 1660, 1747, 1761
AluI AGCT 6 cut(s) 198, 729, 800, 1660, 1747, 1761
Alw26I GTCTC 4 cut(s) 1040, 1209, 1331, 1925
AlwI GGATC 3 cut(s) 1017, 1211, 1652
Ama87I CYCGRG 2 cut(s) 1085, 1199
Aor51HI AGCGCT 1 cut(s) 1621
AoxI GGCC 3 cut(s) 328, 1609, 1723
ApoI RAATTY 1 cut(s) 39
Asp700I GAANNNNTTC 2 cut(s) 270, 1170
AspLEI GCGC 4 cut(s) 172, 805, 1588, 1622
AspS9I GGNCC 3 cut(s) 329, 1427, 1635
AsuC2I CCSGG 5 cut(s) 490, 1086, 1087, 1700, 1974
AsuHPI GGTGA 6 cut(s) 196, 574, 1081, 1103, 1252, 1616
AsuII TTCGAA 1 cut(s) 302
AvaI CYCGRG 2 cut(s) 1085, 1199
AvaII GGWCC 2 cut(s) 1427, 1635
AxyI CCTNAGG 1 cut(s) 60
BaeGI GKGCMC 1 cut(s) 1882
BalI TGGCCA 1 cut(s) 1725
BanI GGYRCC 2 cut(s) 1585, 1909
BanII GRGCYC 2 cut(s) 979, 1514
BauI CACGAG 1 cut(s) 1574
BbsI GAAGAC 1 cut(s) 194
BbvCI CCTCAGC 1 cut(s) 851
BccI CCATC 5 cut(s) 11, 163, 812, 1066, 1244
BceAI ACGGC 2 cut(s) 1065, 1280
BciT130I CCWGG 4 cut(s) 1122, 1310, 1771, 1907
BclI TGATCA 1 cut(s) 1779
BcnI CCSGG 5 cut(s) 490, 1086, 1087, 1700, 1974
BcoDI GTCTC 4 cut(s) 1040, 1209, 1331, 1925
BfaI CTAG 4 cut(s) 453, 1149, 1175, 1548
BfmI CTRYAG 2 cut(s) 688, 1623
BfoI RGCGCY 2 cut(s) 1589, 1623
BfrI CTTAAG 1 cut(s) 1327
BfuAI ACCTGC 1 cut(s) 1104
BglII AGATCT 1 cut(s) 1316
BmcAI AGTACT 1 cut(s) 1580
Bme1390I CCNGG 9 cut(s) 490, 1086, 1087, 1122, 1310, 1700, 1771, 1907, 1974
Bme18I GGWCC 2 cut(s) 1427, 1635
BmeRI GACNNNNNGTC 1 cut(s) 99
BmeT110I CYCGRG 2 cut(s) 1085, 1199
BmgT120I GGNCC 3 cut(s) 329, 1427, 1635
BmiI GGNNCC 4 cut(s) 471, 1587, 1803, 1911
BmrFI CCNGG 9 cut(s) 490, 1086, 1087, 1122, 1310, 1700, 1771, 1907, 1974
BmrI ACTGGG 3 cut(s) 99, 836, 869
BmsI GCATC 5 cut(s) 421, 693, 1383, 1819, 1959
BmuI ACTGGG 3 cut(s) 99, 836, 869
BpiI GAAGAC 1 cut(s) 194
BplI GAGNNNNNCTC 2 cut(s) 713, 745
BpmI CTGGAG 1 cut(s) 715
Bpu10I CCTNAGC 1 cut(s) 851
Bpu14I TTCGAA 1 cut(s) 302
BpuMI CCSGG 5 cut(s) 490, 1086, 1087, 1700, 1974
BsaHI GRCGYC 1 cut(s) 1586
BsaI GGTCTC 1 cut(s) 1209
BsaJI CCNNGG 8 cut(s) 1085, 1086, 1121, 1179, 1308, 1612, 1770, 1905
BsaXI ACNNNNNCTCC 2 cut(s) 79, 109
Bsc4I CCNNNNNNNGG 6 cut(s) 65, 90, 479, 1309, 1685, 1961
Bse1I ACTGG 8 cut(s) 15, 94, 416, 706, 732, 831, 864, 954
Bse21I CCTNAGG 1 cut(s) 60
Bse3DI GCAATG 1 cut(s) 751
BseBI CCWGG 4 cut(s) 1122, 1310, 1771, 1907
BseDI CCNNGG 8 cut(s) 1085, 1086, 1121, 1179, 1308, 1612, 1770, 1905
BseGI GGATG 6 cut(s) 155, 412, 869, 1012, 1374, 1852
BseLI CCNNNNNNNGG 6 cut(s) 65, 90, 479, 1309, 1685, 1961
BseMI GCAATG 1 cut(s) 751
BseMII CTCAG 6 cut(s) 51, 122, 545, 842, 992, 1120
BseNI ACTGG 8 cut(s) 15, 94, 416, 706, 732, 831, 864, 954
BseRI GAGGAG 1 cut(s) 87
BseSI GKGCMC 1 cut(s) 1882
BsgI GTGCAG 1 cut(s) 36
Bsh1236I CGCG 1 cut(s) 805
BshFI GGCC 3 cut(s) 330, 1611, 1725
BshNI GGYRCC 2 cut(s) 1585, 1909
BsiHKCI CYCGRG 2 cut(s) 1085, 1199
BsiSI CCGG 5 cut(s) 489, 1086, 1400, 1699, 1973
BslFI GGGAC 3 cut(s) 106, 1068, 1787
BslI CCNNNNNNNGG 6 cut(s) 65, 90, 479, 1309, 1685, 1961
BsmAI GTCTC 4 cut(s) 1040, 1209, 1331, 1925
BsmBI CGTCTC 1 cut(s) 1040
BsmFI GGGAC 3 cut(s) 106, 1068, 1787
BsnI GGCC 3 cut(s) 330, 1611, 1725
Bso31I GGTCTC 1 cut(s) 1209
BsoBI CYCGRG 2 cut(s) 1085, 1199
Bsp119I TTCGAA 1 cut(s) 302
Bsp1286I GDGCHC 3 cut(s) 979, 1514, 1882
Bsp1407I TGTACA 2 cut(s) 753, 1892
Bsp143I GATC 9 cut(s) 658, 806, 1009, 1203, 1316, 1467, 1550, 1644, 1779
Bsp19I CCATGG 1 cut(s) 1612
BspACI CCGC 3 cut(s) 56, 791, 1828
BspANI GGCC 3 cut(s) 330, 1611, 1725
BspCNI CTCAG 6 cut(s) 52, 121, 544, 843, 991, 1119
BspFNI CGCG 1 cut(s) 805
BspHI TCATGA 1 cut(s) 814
BspLI GGNNCC 4 cut(s) 471, 1587, 1803, 1911
BspMI ACCTGC 1 cut(s) 1104
BspPI GGATC 3 cut(s) 1017, 1211, 1652
BspT104I TTCGAA 1 cut(s) 302
BspT107I GGYRCC 2 cut(s) 1585, 1909
BspTI CTTAAG 1 cut(s) 1327
BspTNI GGTCTC 1 cut(s) 1209
BsrDI GCAATG 1 cut(s) 751
BsrGI TGTACA 2 cut(s) 753, 1892
BsrI ACTGG 8 cut(s) 15, 94, 416, 706, 732, 831, 864, 954
BssECI CCNNGG 8 cut(s) 1085, 1086, 1121, 1179, 1308, 1612, 1770, 1905
BssMI GATC 9 cut(s) 658, 806, 1009, 1203, 1316, 1467, 1550, 1644, 1779
BssNAI GTATAC 2 cut(s) 1035, 1498
BssNI GRCGYC 1 cut(s) 1586
BssSI CACGAG 1 cut(s) 1574
BssT1I CCWWGG 2 cut(s) 1179, 1612
Bst1107I GTATAC 2 cut(s) 1035, 1498
Bst2BI CACGAG 1 cut(s) 1574
Bst2UI CCWGG 4 cut(s) 1122, 1310, 1771, 1907
Bst4CI ACNGT 3 cut(s) 464, 521, 539
Bst6I CTCTTC 1 cut(s) 261
BstACI GRCGYC 1 cut(s) 1586
BstAFI CTTAAG 1 cut(s) 1327
BstAUI TGTACA 2 cut(s) 753, 1892
BstBI TTCGAA 1 cut(s) 302
BstC8I GCNNGC 8 cut(s) 257, 526, 798, 1101, 1143, 1368, 1372, 1727
BstDEI CTNAG 7 cut(s) 60, 108, 531, 851, 971, 978, 1106
BstDSI CCRYGG 1 cut(s) 1612
BstEII GGTNACC 1 cut(s) 1091
BstENI CCTNNNNNAGG 2 cut(s) 63, 477
BstF5I GGATG 6 cut(s) 155, 412, 869, 1012, 1374, 1852
BstFNI CGCG 1 cut(s) 805
BstH2I RGCGCY 2 cut(s) 1589, 1623
BstHHI GCGC 4 cut(s) 172, 805, 1588, 1622
BstKTI GATC 9 cut(s) 661, 809, 1012, 1206, 1319, 1470, 1553, 1647, 1782
BstMAI GTCTC 4 cut(s) 1040, 1209, 1331, 1925
BstMBI GATC 9 cut(s) 658, 806, 1009, 1203, 1316, 1467, 1550, 1644, 1779
BstMWI GCNNNNNNNGC 8 cut(s) 702, 705, 714, 723, 797, 802, 950, 1617
BstNI CCWGG 4 cut(s) 1122, 1310, 1771, 1907
BstNSI RCATGY 5 cut(s) 259, 1103, 1145, 1374, 1422
BstPI GGTNACC 1 cut(s) 1091
BstSCI CCNGG 9 cut(s) 488, 1084, 1085, 1120, 1308, 1698, 1769, 1905, 1972
BstSFI CTRYAG 2 cut(s) 688, 1623
BstSLI GKGCMC 1 cut(s) 1882
BstUI CGCG 1 cut(s) 805
BstV2I GAAGAC 1 cut(s) 194
BstX2I RGATCY 2 cut(s) 1316, 1644
BstYI RGATCY 2 cut(s) 1316, 1644
BstZ17I GTATAC 2 cut(s) 1035, 1498
Bsu36I CCTNAGG 1 cut(s) 60
BsuRI GGCC 3 cut(s) 330, 1611, 1725
BtgI CCRYGG 1 cut(s) 1612
BtgZI GCGATG 2 cut(s) 723, 732
BtsCI GGATG 6 cut(s) 155, 412, 869, 1012, 1374, 1852
BtsI GCAGTG 1 cut(s) 619
BtsIMutI CAGTG 7 cut(s) 8, 517, 604, 619, 699, 824, 857
BveI ACCTGC 1 cut(s) 1104
Cac8I GCNNGC 8 cut(s) 257, 526, 798, 1101, 1143, 1368, 1372, 1727
CciI TCATGA 1 cut(s) 814
CfoI GCGC 4 cut(s) 172, 805, 1588, 1622
Cfr13I GGNCC 3 cut(s) 329, 1427, 1635
Cfr9I CCCGGG 1 cut(s) 1085
Csp6I GTAC 3 cut(s) 754, 1579, 1893
CspCI CAANNNNNGTGG 2 cut(s) 1405, 1440
CviQI GTAC 3 cut(s) 754, 1579, 1893
DdeI CTNAG 7 cut(s) 60, 108, 531, 851, 971, 978, 1106
DinI GGCGCC 1 cut(s) 1587
DpnI GATC 9 cut(s) 660, 808, 1011, 1205, 1318, 1469, 1552, 1646, 1781
DpnII GATC 9 cut(s) 658, 806, 1009, 1203, 1316, 1467, 1550, 1644, 1779
DriI GACNNNNNGTC 1 cut(s) 99
EaeI YGGCCR 1 cut(s) 1723
Eam1104I CTCTTC 1 cut(s) 261
Eam1105I GACNNNNNGTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 261
Eco130I CCWWGG 2 cut(s) 1179, 1612
Eco24I GRGCYC 2 cut(s) 979, 1514
Eco31I GGTCTC 1 cut(s) 1209
Eco32I GATATC 1 cut(s) 443
Eco47I GGWCC 2 cut(s) 1427, 1635
Eco47III AGCGCT 1 cut(s) 1621
Eco57I CTGAAG 1 cut(s) 1185
Eco81I CCTNAGG 1 cut(s) 60
Eco88I CYCGRG 2 cut(s) 1085, 1199
Eco91I GGTNACC 1 cut(s) 1091
EcoNI CCTNNNNNAGG 2 cut(s) 63, 477
EcoO65I GGTNACC 1 cut(s) 1091
EcoRII CCWGG 4 cut(s) 1120, 1308, 1769, 1905
EcoRV GATATC 1 cut(s) 443
EcoT14I CCWWGG 2 cut(s) 1179, 1612
EcoT22I ATGCAT 1 cut(s) 1376
EcoT38I GRGCYC 2 cut(s) 979, 1514
EgeI GGCGCC 1 cut(s) 1587
EheI GGCGCC 1 cut(s) 1587
ErhI CCWWGG 2 cut(s) 1179, 1612
Esp3I CGTCTC 1 cut(s) 1040
FalI AAGNNNNNCTT 2 cut(s) 258, 290
FaqI GGGAC 3 cut(s) 106, 1068, 1787
FauI CCCGC 1 cut(s) 1821
FauNDI CATATG 2 cut(s) 279, 1194
FbaI TGATCA 1 cut(s) 1779
FblI GTMKAC 2 cut(s) 1034, 1497
FokI GGATG 6 cut(s) 142, 399, 876, 999, 1361, 1839
FriOI GRGCYC 2 cut(s) 979, 1514
FspBI CTAG 4 cut(s) 453, 1149, 1175, 1548
FspI TGCGCA 1 cut(s) 171
GlaI GCGC 4 cut(s) 171, 804, 1587, 1621
GsuI CTGGAG 1 cut(s) 715
HaeII RGCGCY 2 cut(s) 1589, 1623
HaeIII GGCC 3 cut(s) 330, 1611, 1725
HapII CCGG 5 cut(s) 489, 1086, 1400, 1699, 1973
HhaI GCGC 4 cut(s) 172, 805, 1588, 1622
Hin1I GRCGYC 1 cut(s) 1586
Hin6I GCGC 4 cut(s) 170, 803, 1586, 1620
HinP1I GCGC 4 cut(s) 170, 803, 1586, 1620
HinfI GANTC 4 cut(s) 94, 431, 449, 1933
HpaII CCGG 5 cut(s) 489, 1086, 1400, 1699, 1973
HphI GGTGA 6 cut(s) 196, 574, 1081, 1103, 1252, 1616
Hpy166II GTNNAC 5 cut(s) 1035, 1430, 1498, 1635, 1790
Hpy188I TCNGA 8 cut(s) 372, 534, 981, 1109, 1138, 1270, 1670, 1784
Hpy188III TCNNGA 7 cut(s) 132, 446, 815, 1007, 1201, 1742, 1937
Hpy8I GTNNAC 5 cut(s) 1035, 1430, 1498, 1635, 1790
Hpy99I CGWCG 1 cut(s) 154
HpyAV CCTTC 9 cut(s) 86, 90, 114, 123, 134, 922, 1601, 1761, 2001
HpyCH4III ACNGT 3 cut(s) 464, 521, 539
HpyF10VI GCNNNNNNNGC 8 cut(s) 702, 705, 714, 723, 797, 802, 950, 1617
HpyF3I CTNAG 7 cut(s) 60, 108, 531, 851, 971, 978, 1106
Hsp92I GRCGYC 1 cut(s) 1586
HspAI GCGC 4 cut(s) 170, 803, 1586, 1620
KasI GGCGCC 1 cut(s) 1585
Ksp22I TGATCA 1 cut(s) 1779
Kzo9I GATC 9 cut(s) 658, 806, 1009, 1203, 1316, 1467, 1550, 1644, 1779
LmnI GCTCC 5 cut(s) 734, 835, 1138, 1801, 1832
LweI GCATC 5 cut(s) 421, 693, 1383, 1819, 1959
MaeI CTAG 4 cut(s) 453, 1149, 1175, 1548
MaeIII GTNAC 3 cut(s) 6, 643, 1091
MalI GATC 9 cut(s) 660, 808, 1011, 1205, 1318, 1469, 1552, 1646, 1781
MboI GATC 9 cut(s) 658, 806, 1009, 1203, 1316, 1467, 1550, 1644, 1779
MflI RGATCY 2 cut(s) 1316, 1644
MhlI GDGCHC 3 cut(s) 979, 1514, 1882
MlsI TGGCCA 1 cut(s) 1725
MluCI AATT 7 cut(s) 25, 39, 298, 649, 1873, 1884, 1999
MluNI TGGCCA 1 cut(s) 1725
Mly113I GGCGCC 1 cut(s) 1586
MlyI GAGTC 2 cut(s) 88, 1927
Mox20I TGGCCA 1 cut(s) 1725
Mph1103I ATGCAT 1 cut(s) 1376
MroXI GAANNNNTTC 2 cut(s) 270, 1170
MscI TGGCCA 1 cut(s) 1725
MseI TTAA 9 cut(s) 24, 345, 420, 1286, 1328, 1599, 1713, 1845, 1958
MslI CAYNNNNRTG 2 cut(s) 816, 862
Msp20I TGGCCA 1 cut(s) 1725
MspCI CTTAAG 1 cut(s) 1327
MspI CCGG 5 cut(s) 489, 1086, 1400, 1699, 1973
MspR9I CCNGG 9 cut(s) 490, 1086, 1087, 1122, 1310, 1700, 1771, 1907, 1974
MvaI CCWGG 4 cut(s) 1122, 1310, 1771, 1907
MvnI CGCG 1 cut(s) 805
MwoI GCNNNNNNNGC 8 cut(s) 702, 705, 714, 723, 797, 802, 950, 1617
NarI GGCGCC 1 cut(s) 1586
NciI CCSGG 5 cut(s) 490, 1086, 1087, 1700, 1974
NcoI CCATGG 1 cut(s) 1612
NdeI CATATG 2 cut(s) 279, 1194
NdeII GATC 9 cut(s) 658, 806, 1009, 1203, 1316, 1467, 1550, 1644, 1779
NlaIV GGNNCC 4 cut(s) 471, 1587, 1803, 1911
NmuCI GTSAC 2 cut(s) 6, 1091
NsbI TGCGCA 1 cut(s) 171
NsiI ATGCAT 1 cut(s) 1376
NspI RCATGY 5 cut(s) 259, 1103, 1145, 1374, 1422
NspV TTCGAA 1 cut(s) 302
PaeI GCATGC 4 cut(s) 259, 1103, 1145, 1374
PagI TCATGA 1 cut(s) 814
PciI ACATGT 1 cut(s) 1418
PdmI GAANNNNTTC 2 cut(s) 270, 1170
PfeI GAWTC 2 cut(s) 431, 449
PleI GAGTC 2 cut(s) 88, 1927
PluTI GGCGCC 1 cut(s) 1589
PpsI GAGTC 2 cut(s) 88, 1927
PscI ACATGT 1 cut(s) 1418
PsiI TTATAA 1 cut(s) 477
Psp6I CCWGG 4 cut(s) 1120, 1308, 1769, 1905
PspEI GGTNACC 1 cut(s) 1091
PspGI CCWGG 4 cut(s) 1120, 1308, 1769, 1905
PspN4I GGNNCC 4 cut(s) 471, 1587, 1803, 1911
PspPI GGNCC 3 cut(s) 329, 1427, 1635
PsuI RGATCY 2 cut(s) 1316, 1644
RsaI GTAC 3 cut(s) 755, 1580, 1894
RsaNI GTAC 3 cut(s) 754, 1579, 1893
RseI CAYNNNNRTG 2 cut(s) 816, 862
SaqAI TTAA 9 cut(s) 24, 345, 420, 1286, 1328, 1599, 1713, 1845, 1958
Sau3AI GATC 9 cut(s) 658, 806, 1009, 1203, 1316, 1467, 1550, 1644, 1779
Sau96I GGNCC 3 cut(s) 329, 1427, 1635
ScaI AGTACT 1 cut(s) 1580
SchI GAGTC 2 cut(s) 88, 1927
ScrFI CCNGG 9 cut(s) 490, 1086, 1087, 1122, 1310, 1700, 1771, 1907, 1974
SduI GDGCHC 3 cut(s) 979, 1514, 1882
SfaNI GCATC 5 cut(s) 421, 693, 1383, 1819, 1959
SfcI CTRYAG 2 cut(s) 688, 1623
SfoI GGCGCC 1 cut(s) 1587
SfuI TTCGAA 1 cut(s) 302
SinI GGWCC 2 cut(s) 1427, 1635
SmaI CCCGGG 1 cut(s) 1087
SmiMI CAYNNNNRTG 2 cut(s) 816, 862
SmlI CTYRAG 1 cut(s) 1327
SmoI CTYRAG 1 cut(s) 1327
SphI GCATGC 4 cut(s) 259, 1103, 1145, 1374
Sse9I AATT 7 cut(s) 25, 39, 298, 649, 1873, 1884, 1999
SsiI CCGC 3 cut(s) 56, 791, 1828
SspDI GGCGCC 1 cut(s) 1585
SspI AATATT 1 cut(s) 1187
SspMI CTAG 4 cut(s) 453, 1149, 1175, 1548
StyD4I CCNGG 9 cut(s) 488, 1084, 1085, 1120, 1308, 1698, 1769, 1905, 1972
StyI CCWWGG 2 cut(s) 1179, 1612
TaaI ACNGT 3 cut(s) 464, 521, 539
TaqI TCGA 4 cut(s) 302, 318, 434, 1275
TasI AATT 7 cut(s) 25, 39, 298, 649, 1873, 1884, 1999
TatI WGTACW 3 cut(s) 753, 1578, 1892
TfiI GAWTC 2 cut(s) 431, 449
Tru1I TTAA 9 cut(s) 24, 345, 420, 1286, 1328, 1599, 1713, 1845, 1958
Tru9I TTAA 9 cut(s) 24, 345, 420, 1286, 1328, 1599, 1713, 1845, 1958
TscAI CASTG 7 cut(s) 15, 524, 604, 619, 706, 831, 864
TseFI GTSAC 2 cut(s) 6, 1091
Tsp45I GTSAC 2 cut(s) 6, 1091
TspDTI ATGAA 3 cut(s) 105, 266, 941
TspGWI ACGGA 3 cut(s) 202, 373, 1845
TspMI CCCGGG 1 cut(s) 1085
TspRI CASTG 7 cut(s) 15, 524, 604, 619, 706, 831, 864
Vha464I CTTAAG 1 cut(s) 1327
VpaK11BI GGWCC 2 cut(s) 1427, 1635
XagI CCTNNNNNAGG 2 cut(s) 63, 477
XapI RAATTY 1 cut(s) 39
XceI RCATGY 5 cut(s) 259, 1103, 1145, 1374, 1422
XcmI CCANNNNNNNNNTGG 1 cut(s) 931
XmaI CCCGGG 1 cut(s) 1085
XmiI GTMKAC 2 cut(s) 1034, 1497
XmnI GAANNNNTTC 2 cut(s) 270, 1170
XspI CTAG 4 cut(s) 453, 1149, 1175, 1548
ZrmI AGTACT 1 cut(s) 1580
Zsp2I ATGCAT 1 cut(s) 1376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.