MD17G1026400.v1.1

Protein kinase PVPK-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007009
Physical Location & Seq
Reverse (-)
1894005 .. 1897850
3846 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1026400.v1.1.491

Sequence Viewer

Length: 2025 bp
ATGGAGTCACTGGTTGACGGGGTTAATTCTTTGCCGAAGATTCGCTATTCTGCACCGCCGGAGGTAGGCAACAACCCTCCTTCCACAACTGGGACTGCTCGTCCTTCTCAGCCCCCATCTCCAAAACAATCGAGAAGCGAAGGGGTTCTGTCGCCATCTTGCGTGACTGCGCATACTCATTCGGTAAAGACAGTCAGCTATTCAAATGGTTCTGTAAATAATCTAAAGAATCCCAACAAGGCAACTAATCATAGCATGCAGCAGGAAGAGTTTCTCCATATGGGGAAACATTACCACAGTTCGAATAAAGGAAAACTCGAACATGGAGGCCCAAATGATGTTCTTAACAAATCAGCCGAAACTTCTTATCTGAACAAGGTTTCCATGCCGGAAGCAAAATCAAGTGACAAGAATCCGGTTAATGATTGTGATTCGAGTAGATATCTGGAATCTAGAAATCACGGTTTGGTTCCTTATAAAGTAGTGGCCGGGCAGAAAAATAGCCACTTCGTTTCTCACTGTGCAAGCCCTCAGAACAGTTTATATTCTGCCTCTCTGTATTGTGAAGCCAAGCAGAGTTTCACCAACACAGAAGTCAGTGAATGTGCCAGCAGTGTGGAGAAGTCTGTTGAAAGCGGCGAAGTTACTAATTCCTGTGATCTTATTGAGAGCAGGACGACCAGCATCTACAGAGGCAGCACTGGCAGTGATGTTAGCGATGAGAGCAGCTCCAGTAGTTTGAGCAATGCCATTTACAAGCCCCACAAGGCAAATGATGTTAGATGGGAAGCAGTGCAAGCTGCGCGATCTGATCATGATGGAACGCTGGGTAAGGAGCATTTCAAGTTGCTGAGGACACTGGGATGTGGAGATATAGGCAGCGTTTATTTAGCTGAACTGATTGGCACTAAAACTTACTTCGCCATGAAGGTTATGGATAAAGCATTATTGGCAAGTAGGAAAAAGCTTCTTAGGGCTCAGACAGAGAGGGAGATACTGCAATCTCTGGATCATCCTTTCCTGCCAACATTATATACACACTTTGAGACGGAGAAGGTATCTTGTTTGGTGATGGAGTTTTGTCCTGGGGGTGATCTGCACGCACTCAGACAAAAACAACCTGGGAAACATTTTCCGGAGCATGCTGCCAGGTTTTATGTGGCTGAAGTTCTTCTTGCTTTGGAGTATTTGCATATGCTCGGGATCATTTACAGAGACCTCAAACCAGAGAATGTTTTGGTGAGGGAAGATGGACATATAATGCTTTCAGATTTCGACCTTTCTTTAAGATGCGCCGTTTCCCCCACCCTGGTAAGATCTTCAAACTTAAGCTTGGAGACAAAGAAGTCATCATATTGTGTTCAGCCTGCATGCATCCAGCCGACTTGTGTGATGCAGCCAGATTGCATTACCCCCACATGTTTTGGTCCACGCTTTTTCACGGGCAAGCCAAAGAAAGATAAAAAGACTAAAGTGAAGAAGAATAATAATGATGTAAACAATCAAGCGAGCCATCTCCCCGAGCTCGTTGCAGAACCAACGAGTGCTAGATCAATGTCCTTTGTGGGAACACACGAGTACTTGGCGCCTGAAATCATTAAAGGTGAAGGCCATGGCAGCGCTGTGGATTGGTGGACATTTGGGATCTTTCTCTACGAGCTTTTGTTCGGAAGAACTCCATTCAAGGGGCAAGGAAATCGGGCTACGTTGTTCAATGTGGTTGGCCAGCCATTACGATTTCCAGAGTCGCCTTCTGTCAGCTTTGCAGCCAGGGACCTGATCAGAGGTTTACTTGTCAAAGAACCACAGCATCGTCTTGCATATAGGAGGGGAGCAACAGAAGTTAAACAGCATCCGTTTTTCCAAAGCGTGAATTGGGCACTAATTCGCTGTACAACTCCACCCCAGGTGCCAAAGCACTACATACTGGACACTCCTGATACACCGAAAGAACCCATGAACGGGAAGATGCCGGGCGTTGATTTGAAGCCATCTGGTAATTATTTAGAGATGGATTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

675

Amino Acids

74.66

Weight (kDa)

8.51

Isoelectric Point (pI)

44.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 282 - 432 5.4e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 282 - 431 2.9e-19 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 518 - 621 9.1e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 525 - 608 5.7e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 477
AasI GACNNNNNNGTC 1 cut(s) 1345
Acc16I TGCGCA 1 cut(s) 171
AccB1I GGYRCC 2 cut(s) 1585, 1909
AccII CGCG 1 cut(s) 805
AccIII TCCGGA 1 cut(s) 1135
AciI CCGC 2 cut(s) 56, 636
AclWI GGATC 3 cut(s) 1017, 1211, 1652
AcoI YGGCCR 2 cut(s) 486, 1723
AcuI CTGAAG 1 cut(s) 1185
AcyI GRCGYC 1 cut(s) 1586
AfaI GTAC 2 cut(s) 1580, 1894
AfeI AGCGCT 1 cut(s) 1621
AfiI CCNNNNNNNGG 6 cut(s) 65, 90, 1309, 1685, 1961, 1962
AflII CTTAAG 1 cut(s) 1327
AflIII ACRYGT 1 cut(s) 1418
AgsI TTSAA 7 cut(s) 204, 632, 844, 1323, 1684, 1714, 1987
AhdI GACNNNNNGTC 1 cut(s) 99
AjnI CCWGG 6 cut(s) 1084, 1120, 1148, 1308, 1769, 1905
AjuI GAANNNNNNNTTGG 2 cut(s) 563, 595
AloI GAACNNNNNNTCC 2 cut(s) 365, 397
AluBI AGCT 9 cut(s) 198, 729, 800, 893, 967, 1332, 1525, 1660, 1761
AluI AGCT 9 cut(s) 198, 729, 800, 893, 967, 1332, 1525, 1660, 1761
Alw21I GWGCWC 1 cut(s) 1527
Alw26I GTCTC 3 cut(s) 1040, 1209, 1331
AlwI GGATC 3 cut(s) 1017, 1211, 1652
Ama87I CYCGRG 2 cut(s) 1199, 1520
Aor13HI TCCGGA 1 cut(s) 1135
Aor51HI AGCGCT 1 cut(s) 1621
AoxI GGCC 4 cut(s) 328, 486, 1609, 1723
ApeKI GCWGC 9 cut(s) 259, 696, 726, 800, 879, 1145, 1396, 1617, 1766
Asp700I GAANNNNTTC 3 cut(s) 144, 270, 1170
AspLEI GCGC 5 cut(s) 172, 805, 1295, 1588, 1622
AspS9I GGNCC 3 cut(s) 329, 1427, 1774
AsuC2I CCSGG 2 cut(s) 490, 1974
AsuHPI GGTGA 5 cut(s) 574, 1081, 1103, 1252, 1616
AsuII TTCGAA 1 cut(s) 302
AvaI CYCGRG 2 cut(s) 1199, 1520
AvaII GGWCC 2 cut(s) 1427, 1774
BaeGI GKGCMC 1 cut(s) 1882
BalI TGGCCA 1 cut(s) 1725
BanI GGYRCC 2 cut(s) 1585, 1909
BanII GRGCYC 2 cut(s) 979, 1527
BauI CACGAG 1 cut(s) 1574
Bbv12I GWGCWC 1 cut(s) 1527
BbvCI CCTCAGC 1 cut(s) 851
BbvI GCAGC 9 cut(s) 271, 708, 738, 787, 891, 1132, 1408, 1629, 1778
BccI CCATC 9 cut(s) 124, 163, 777, 812, 1066, 1244, 1521, 1999, 2005
BceAI ACGGC 1 cut(s) 1280
BcgI CGANNNNNNTGC 4 cut(s) 1511, 1545, 1679, 1713
BciT130I CCWGG 6 cut(s) 1086, 1122, 1150, 1310, 1771, 1907
BclI TGATCA 2 cut(s) 811, 1779
BcnI CCSGG 2 cut(s) 490, 1974
BcoDI GTCTC 3 cut(s) 1040, 1209, 1331
BfaI CTAG 2 cut(s) 453, 1548
BfmI CTRYAG 1 cut(s) 688
BfoI RGCGCY 2 cut(s) 1589, 1623
BfrI CTTAAG 1 cut(s) 1327
BglII AGATCT 1 cut(s) 1316
BmcAI AGTACT 1 cut(s) 1580
Bme1390I CCNGG 8 cut(s) 490, 1086, 1122, 1150, 1310, 1771, 1907, 1974
Bme18I GGWCC 2 cut(s) 1427, 1774
BmeRI GACNNNNNGTC 1 cut(s) 99
BmeT110I CYCGRG 2 cut(s) 1199, 1520
BmgT120I GGNCC 3 cut(s) 329, 1427, 1774
BmiI GGNNCC 4 cut(s) 471, 1587, 1775, 1911
BmrFI CCNGG 8 cut(s) 490, 1086, 1122, 1150, 1310, 1771, 1907, 1974
BmrI ACTGGG 2 cut(s) 99, 869
BmsI GCATC 7 cut(s) 693, 1280, 1383, 1383, 1819, 1861, 1959
BmuI ACTGGG 2 cut(s) 99, 869
BplI GAGNNNNNCTC 2 cut(s) 713, 745
BpmI CTGGAG 1 cut(s) 715
Bpu10I CCTNAGC 1 cut(s) 851
Bpu14I TTCGAA 1 cut(s) 302
BpuMI CCSGG 2 cut(s) 490, 1974
BsaHI GRCGYC 1 cut(s) 1586
BsaI GGTCTC 1 cut(s) 1209
BsaJI CCNNGG 6 cut(s) 1085, 1121, 1308, 1612, 1770, 1905
BsaWI WCCGGW 2 cut(s) 415, 1135
Bsc4I CCNNNNNNNGG 6 cut(s) 65, 90, 1309, 1685, 1961, 1962
Bse1I ACTGG 6 cut(s) 15, 94, 706, 732, 864, 1932
Bse3DI GCAATG 1 cut(s) 751
BseAI TCCGGA 1 cut(s) 1135
BseBI CCWGG 6 cut(s) 1086, 1122, 1150, 1310, 1771, 1907
BseDI CCNNGG 6 cut(s) 1085, 1121, 1308, 1612, 1770, 1905
BseGI GGATG 4 cut(s) 869, 1012, 1374, 1852
BseLI CCNNNNNNNGG 6 cut(s) 65, 90, 1309, 1685, 1961, 1962
BseMI GCAATG 1 cut(s) 751
BseMII CTCAG 5 cut(s) 122, 545, 842, 992, 1120
BseNI ACTGG 6 cut(s) 15, 94, 706, 732, 864, 1932
BseSI GKGCMC 1 cut(s) 1882
BseXI GCAGC 9 cut(s) 271, 708, 738, 787, 891, 1132, 1408, 1629, 1778
BseYI CCCAGC 1 cut(s) 826
BsgI GTGCAG 2 cut(s) 36, 1082
Bsh1236I CGCG 1 cut(s) 805
BshFI GGCC 4 cut(s) 330, 488, 1611, 1725
BshNI GGYRCC 2 cut(s) 1585, 1909
BsiHKAI GWGCWC 1 cut(s) 1527
BsiHKCI CYCGRG 2 cut(s) 1199, 1520
BsiSI CCGG 6 cut(s) 59, 389, 416, 489, 1136, 1973
BslFI GGGAC 2 cut(s) 106, 1787
BslI CCNNNNNNNGG 6 cut(s) 65, 90, 1309, 1685, 1961, 1962
BsmAI GTCTC 3 cut(s) 1040, 1209, 1331
BsmBI CGTCTC 1 cut(s) 1040
BsmFI GGGAC 2 cut(s) 106, 1787
BsnI GGCC 4 cut(s) 330, 488, 1611, 1725
Bso31I GGTCTC 1 cut(s) 1209
BsoBI CYCGRG 2 cut(s) 1199, 1520
Bsp119I TTCGAA 1 cut(s) 302
Bsp1286I GDGCHC 3 cut(s) 979, 1527, 1882
Bsp13I TCCGGA 1 cut(s) 1135
Bsp1407I TGTACA 1 cut(s) 1892
Bsp19I CCATGG 1 cut(s) 1612
BspACI CCGC 2 cut(s) 56, 636
BspANI GGCC 4 cut(s) 330, 488, 1611, 1725
BspCNI CTCAG 5 cut(s) 121, 544, 843, 991, 1119
BspEI TCCGGA 1 cut(s) 1135
BspFNI CGCG 1 cut(s) 805
BspHI TCATGA 1 cut(s) 814
BspLI GGNNCC 4 cut(s) 471, 1587, 1775, 1911
BspPI GGATC 3 cut(s) 1017, 1211, 1652
BspT104I TTCGAA 1 cut(s) 302
BspT107I GGYRCC 2 cut(s) 1585, 1909
BspTI CTTAAG 1 cut(s) 1327
BspTNI GGTCTC 1 cut(s) 1209
BsrDI GCAATG 1 cut(s) 751
BsrGI TGTACA 1 cut(s) 1892
BsrI ACTGG 6 cut(s) 15, 94, 706, 732, 864, 1932
BssECI CCNNGG 6 cut(s) 1085, 1121, 1308, 1612, 1770, 1905
BssNI GRCGYC 1 cut(s) 1586
BssSI CACGAG 1 cut(s) 1574
BssT1I CCWWGG 1 cut(s) 1612
Bst2BI CACGAG 1 cut(s) 1574
Bst2UI CCWGG 6 cut(s) 1086, 1122, 1150, 1310, 1771, 1907
Bst4CI ACNGT 5 cut(s) 193, 299, 464, 521, 539
Bst6I CTCTTC 1 cut(s) 261
BstACI GRCGYC 1 cut(s) 1586
BstAFI CTTAAG 1 cut(s) 1327
BstAUI TGTACA 1 cut(s) 1892
BstBI TTCGAA 1 cut(s) 302
BstDEI CTNAG 6 cut(s) 108, 531, 851, 971, 978, 1106
BstDSI CCRYGG 1 cut(s) 1612
BstF5I GGATG 4 cut(s) 869, 1012, 1374, 1852
BstFNI CGCG 1 cut(s) 805
BstH2I RGCGCY 2 cut(s) 1589, 1623
BstHHI GCGC 5 cut(s) 172, 805, 1295, 1588, 1622
BstMAI GTCTC 3 cut(s) 1040, 1209, 1331
BstMWI GCNNNNNNNGC 6 cut(s) 702, 723, 797, 802, 950, 1617
BstNI CCWGG 6 cut(s) 1086, 1122, 1150, 1310, 1771, 1907
BstNSI RCATGY 4 cut(s) 259, 1145, 1374, 1422
BstSCI CCNGG 8 cut(s) 488, 1084, 1120, 1148, 1308, 1769, 1905, 1972
BstSFI CTRYAG 1 cut(s) 688
BstSLI GKGCMC 1 cut(s) 1882
BstUI CGCG 1 cut(s) 805
BstV1I GCAGC 9 cut(s) 271, 708, 738, 787, 891, 1132, 1408, 1629, 1778
BstX2I RGATCY 2 cut(s) 1316, 1644
BstXI CCANNNNNNTGG 1 cut(s) 616
BstYI RGATCY 2 cut(s) 1316, 1644
BsuRI GGCC 4 cut(s) 330, 488, 1611, 1725
BtgI CCRYGG 1 cut(s) 1612
BtgZI GCGATG 1 cut(s) 732
BtsCI GGATG 4 cut(s) 869, 1012, 1374, 1852
BtsI GCAGTG 3 cut(s) 619, 712, 798
BtsIMutI CAGTG 8 cut(s) 8, 517, 604, 619, 699, 712, 798, 857
CciI TCATGA 1 cut(s) 814
CfoI GCGC 5 cut(s) 172, 805, 1295, 1588, 1622
Cfr13I GGNCC 3 cut(s) 329, 1427, 1774
Csp6I GTAC 2 cut(s) 1579, 1893
CspCI CAANNNNNGTGG 2 cut(s) 1405, 1440
CviQI GTAC 2 cut(s) 1579, 1893
DdeI CTNAG 6 cut(s) 108, 531, 851, 971, 978, 1106
DinI GGCGCC 1 cut(s) 1587
DrdI GACNNNNNNGTC 1 cut(s) 1345
DriI GACNNNNNGTC 1 cut(s) 99
DseDI GACNNNNNNGTC 1 cut(s) 1345
EaeI YGGCCR 2 cut(s) 486, 1723
Eam1104I CTCTTC 1 cut(s) 261
Eam1105I GACNNNNNGTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 261
Ecl136II GAGCTC 1 cut(s) 1525
Eco130I CCWWGG 1 cut(s) 1612
Eco24I GRGCYC 2 cut(s) 979, 1527
Eco31I GGTCTC 1 cut(s) 1209
Eco32I GATATC 1 cut(s) 443
Eco47I GGWCC 2 cut(s) 1427, 1774
Eco47III AGCGCT 1 cut(s) 1621
Eco53kI GAGCTC 1 cut(s) 1525
Eco57I CTGAAG 1 cut(s) 1185
Eco88I CYCGRG 2 cut(s) 1199, 1520
EcoICRI GAGCTC 1 cut(s) 1525
EcoO109I RGGNCCY 1 cut(s) 1774
EcoRII CCWGG 6 cut(s) 1084, 1120, 1148, 1308, 1769, 1905
EcoRV GATATC 1 cut(s) 443
EcoT14I CCWWGG 1 cut(s) 1612
EcoT22I ATGCAT 1 cut(s) 1376
EcoT38I GRGCYC 2 cut(s) 979, 1527
EgeI GGCGCC 1 cut(s) 1587
EheI GGCGCC 1 cut(s) 1587
ErhI CCWWGG 1 cut(s) 1612
Esp3I CGTCTC 1 cut(s) 1040
FalI AAGNNNNNCTT 2 cut(s) 1158, 1190
FaqI GGGAC 2 cut(s) 106, 1787
FauNDI CATATG 2 cut(s) 279, 1194
FbaI TGATCA 2 cut(s) 811, 1779
FokI GGATG 4 cut(s) 876, 999, 1361, 1839
FriOI GRGCYC 2 cut(s) 979, 1527
FspBI CTAG 2 cut(s) 453, 1548
FspI TGCGCA 1 cut(s) 171
GlaI GCGC 5 cut(s) 171, 804, 1294, 1587, 1621
GsaI CCCAGC 1 cut(s) 830
GsuI CTGGAG 1 cut(s) 715
HaeII RGCGCY 2 cut(s) 1589, 1623
HaeIII GGCC 4 cut(s) 330, 488, 1611, 1725
HapII CCGG 6 cut(s) 59, 389, 416, 489, 1136, 1973
HhaI GCGC 5 cut(s) 172, 805, 1295, 1588, 1622
Hin1I GRCGYC 1 cut(s) 1586
Hin6I GCGC 5 cut(s) 170, 803, 1293, 1586, 1620
HinP1I GCGC 5 cut(s) 170, 803, 1293, 1586, 1620
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HindIII AAGCTT 2 cut(s) 965, 1330
HinfI GANTC 7 cut(s) 5, 40, 229, 412, 431, 449, 1745
HpaII CCGG 6 cut(s) 59, 389, 416, 489, 1136, 1973
HphI GGTGA 5 cut(s) 574, 1081, 1103, 1252, 1616
Hpy166II GTNNAC 5 cut(s) 16, 1430, 1498, 1635, 1790
Hpy188I TCNGA 8 cut(s) 372, 534, 811, 981, 1109, 1270, 1670, 1784
Hpy188III TCNNGA 9 cut(s) 132, 446, 453, 815, 1007, 1136, 1201, 1742, 1937
Hpy8I GTNNAC 5 cut(s) 16, 1430, 1498, 1635, 1790
HpyAV CCTTC 7 cut(s) 90, 114, 134, 922, 1048, 1601, 1761
HpyCH4III ACNGT 5 cut(s) 193, 299, 464, 521, 539
HpyCH4IV ACGT 1 cut(s) 1706
HpyF10VI GCNNNNNNNGC 6 cut(s) 702, 723, 797, 802, 950, 1617
HpyF3I CTNAG 6 cut(s) 108, 531, 851, 971, 978, 1106
HpySE526I ACGT 1 cut(s) 1706
Hsp92I GRCGYC 1 cut(s) 1586
HspAI GCGC 5 cut(s) 170, 803, 1293, 1586, 1620
KasI GGCGCC 1 cut(s) 1585
Kpn2I TCCGGA 1 cut(s) 1135
Ksp22I TGATCA 2 cut(s) 811, 1779
LmnI GCTCC 4 cut(s) 734, 835, 1138, 1832
Lsp1109I GCAGC 9 cut(s) 271, 708, 738, 787, 891, 1132, 1408, 1629, 1778
LweI GCATC 7 cut(s) 693, 1280, 1383, 1383, 1819, 1861, 1959
MaeI CTAG 2 cut(s) 453, 1548
MaeII ACGT 1 cut(s) 1706
MaeIII GTNAC 4 cut(s) 6, 163, 404, 643
MboII GAAGA 9 cut(s) 49, 278, 1163, 1259, 1311, 1489, 1492, 1683, 1978
MflI RGATCY 2 cut(s) 1316, 1644
MhlI GDGCHC 3 cut(s) 979, 1527, 1882
MlsI TGGCCA 1 cut(s) 1725
MluCI AATT 5 cut(s) 25, 649, 1873, 1884, 1999
MluNI TGGCCA 1 cut(s) 1725
Mly113I GGCGCC 1 cut(s) 1586
MlyI GAGTC 2 cut(s) 14, 1754
Mox20I TGGCCA 1 cut(s) 1725
Mph1103I ATGCAT 1 cut(s) 1376
MroI TCCGGA 1 cut(s) 1135
MroXI GAANNNNTTC 3 cut(s) 144, 270, 1170
MscI TGGCCA 1 cut(s) 1725
MseI TTAA 7 cut(s) 24, 345, 420, 1286, 1328, 1599, 1845
MslI CAYNNNNRTG 1 cut(s) 862
Msp20I TGGCCA 1 cut(s) 1725
MspCI CTTAAG 1 cut(s) 1327
MspI CCGG 6 cut(s) 59, 389, 416, 489, 1136, 1973
MspR9I CCNGG 8 cut(s) 490, 1086, 1122, 1150, 1310, 1771, 1907, 1974
MvaI CCWGG 6 cut(s) 1086, 1122, 1150, 1310, 1771, 1907
MvnI CGCG 1 cut(s) 805
MwoI GCNNNNNNNGC 6 cut(s) 702, 723, 797, 802, 950, 1617
NarI GGCGCC 1 cut(s) 1586
NciI CCSGG 2 cut(s) 490, 1974
NcoI CCATGG 1 cut(s) 1612
NdeI CATATG 2 cut(s) 279, 1194
NlaIV GGNNCC 4 cut(s) 471, 1587, 1775, 1911
NmuCI GTSAC 3 cut(s) 6, 163, 404
NsbI TGCGCA 1 cut(s) 171
NsiI ATGCAT 1 cut(s) 1376
NspI RCATGY 4 cut(s) 259, 1145, 1374, 1422
NspV TTCGAA 1 cut(s) 302
PaeI GCATGC 3 cut(s) 259, 1145, 1374
PagI TCATGA 1 cut(s) 814
PciI ACATGT 1 cut(s) 1418
PdmI GAANNNNTTC 3 cut(s) 144, 270, 1170
PfeI GAWTC 5 cut(s) 40, 229, 412, 431, 449
PleI GAGTC 2 cut(s) 13, 1753
PluTI GGCGCC 1 cut(s) 1589
PpsI GAGTC 2 cut(s) 13, 1753
PpuMI RGGWCCY 1 cut(s) 1774
PscI ACATGT 1 cut(s) 1418
PsiI TTATAA 1 cut(s) 477
Psp124BI GAGCTC 1 cut(s) 1527
Psp5II RGGWCCY 1 cut(s) 1774
Psp6I CCWGG 6 cut(s) 1084, 1120, 1148, 1308, 1769, 1905
PspFI CCCAGC 1 cut(s) 826
PspGI CCWGG 6 cut(s) 1084, 1120, 1148, 1308, 1769, 1905
PspN4I GGNNCC 4 cut(s) 471, 1587, 1775, 1911
PspPI GGNCC 3 cut(s) 329, 1427, 1774
PspPPI RGGWCCY 1 cut(s) 1774
PsuI RGATCY 2 cut(s) 1316, 1644
RsaI GTAC 2 cut(s) 1580, 1894
RsaNI GTAC 2 cut(s) 1579, 1893
RseI CAYNNNNRTG 1 cut(s) 862
SacI GAGCTC 1 cut(s) 1527
SaqAI TTAA 7 cut(s) 24, 345, 420, 1286, 1328, 1599, 1845
Sau96I GGNCC 3 cut(s) 329, 1427, 1774
ScaI AGTACT 1 cut(s) 1580
SchI GAGTC 2 cut(s) 14, 1754
ScrFI CCNGG 8 cut(s) 490, 1086, 1122, 1150, 1310, 1771, 1907, 1974
SduI GDGCHC 3 cut(s) 979, 1527, 1882
SfaNI GCATC 7 cut(s) 693, 1280, 1383, 1383, 1819, 1861, 1959
SfcI CTRYAG 1 cut(s) 688
SfoI GGCGCC 1 cut(s) 1587
SfuI TTCGAA 1 cut(s) 302
SinI GGWCC 2 cut(s) 1427, 1774
SmiMI CAYNNNNRTG 1 cut(s) 862
SmlI CTYRAG 1 cut(s) 1327
SmoI CTYRAG 1 cut(s) 1327
SphI GCATGC 3 cut(s) 259, 1145, 1374
Sse9I AATT 5 cut(s) 25, 649, 1873, 1884, 1999
SsiI CCGC 2 cut(s) 56, 636
SspDI GGCGCC 1 cut(s) 1585
SspMI CTAG 2 cut(s) 453, 1548
SstI GAGCTC 1 cut(s) 1527
StyD4I CCNGG 8 cut(s) 488, 1084, 1120, 1148, 1308, 1769, 1905, 1972
StyI CCWWGG 1 cut(s) 1612
TaaI ACNGT 5 cut(s) 193, 299, 464, 521, 539
TaiI ACGT 1 cut(s) 1709
TaqI TCGA 5 cut(s) 131, 302, 318, 434, 1275
TasI AATT 5 cut(s) 25, 649, 1873, 1884, 1999
TatI WGTACW 2 cut(s) 1578, 1892
TauI GCSGC 1 cut(s) 639
TfiI GAWTC 5 cut(s) 40, 229, 412, 431, 449
Tru1I TTAA 7 cut(s) 24, 345, 420, 1286, 1328, 1599, 1845
Tru9I TTAA 7 cut(s) 24, 345, 420, 1286, 1328, 1599, 1845
TscAI CASTG 8 cut(s) 15, 524, 604, 619, 706, 712, 798, 864
TseFI GTSAC 3 cut(s) 6, 163, 404
TseI GCWGC 9 cut(s) 259, 696, 726, 800, 879, 1145, 1396, 1617, 1766
Tsp45I GTSAC 3 cut(s) 6, 163, 404
TspDTI ATGAA 2 cut(s) 941, 1973
TspGWI ACGGA 2 cut(s) 1064, 1845
TspRI CASTG 8 cut(s) 15, 524, 604, 619, 706, 712, 798, 864
Vha464I CTTAAG 1 cut(s) 1327
VpaK11BI GGWCC 2 cut(s) 1427, 1774
XbaI TCTAGA 1 cut(s) 452
XceI RCATGY 4 cut(s) 259, 1145, 1374, 1422
XcmI CCANNNNNNNNNTGG 2 cut(s) 931, 1156
XmnI GAANNNNTTC 3 cut(s) 144, 270, 1170
XspI CTAG 2 cut(s) 453, 1548
ZrmI AGTACT 1 cut(s) 1580
Zsp2I ATGCAT 1 cut(s) 1376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.