FvH4_7g00020

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0013104
Physical Location & Seq
Reverse (-)
615890 .. 618733
2844 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g00020.t2

Sequence Viewer

Length: 555 bp
ATGGAGATGGAGATGGAGATTGTTCATGATCAGCTGACGTTCTGGTGCCACGTGTGCGACATGAGCGTGACTCTGCCGGCGACGACCGTCGCCTGTCCACACTGCTTCAGCGACCTCCTGGAGCTCATGGACTACTCCACTCATGCTCCAGAAGCAGACCGCCTCAACAGCCCACTCCTCCGCCGCCTGATCCACCATCTGTCCGCCGCCGACGTCCTTCCGCCCCCGCCCGCTAATGTCTACTTACTGCCGGCTTCCAAGGCCTACGTAGACGTCATCCCGACCGTGAAGATGACGTCACGTTCAATGACGGTGTGCGCTGTTTGTAAGGAGCAATTTGCGGAGGATGCGGAGGCTAAGCAGCTGGCTTGCAGCCACGTGTACCATCCTGACTGCATCCTCCCTTGGCTTTCTTCCCGCAGTTCATGCCCCCTCTGCCGCTTTCAGCTCCCCACCGACGTCGACCACCCTCCAGACTCCCAACACCGACTTCAACGTCTTGTCCATTACCGTCTTCAACTCCACGACCACCGCAACGCAGGTGGGTTACAGTAA

Protein Analysis

185

Amino Acids

20.8

Weight (kDa)

5.96

Isoelectric Point (pI)

66.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 13 - 43 8.4e-09 zinc-ribbon
zf-rbx1 PF12678 103 - 147 2.5e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 105 - 147 2.9e-12 Ring finger domain
zf-C3HC4_2 PF13923 106 - 146 3.2e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 106 - 133 1.4e-06 RING-like zinc finger
zf-C3HC4 PF00097 106 - 146 1.4e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013104)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44330 AT3G60080
fragaria_vesca FvH4_7g00020 FvH4_7g00020
malus_domestica MD02G1319700.v1.1
prunus_persica Prupe.2G002400_v2.0.a1 Prupe.8G211400_v2.0.a1
pyrus_communis pycom02g26770
rosa_chinensis RchiOBHm_Chr1g0313221
rosa_laevigata RLG00000030812
rosa_multiflora Rmu_sc0013586.1_g000001 Rmu_sc0027690.1_g000001
rosa_roxburghii Rroxscaffold_4G00332330
rosa_rugosa RorugPtG0001800.1
rosa_samantha Rh1AG001800 Rh1CG004000 Rh1DG001700
rosa_wichuraiana Rw1G000090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 530
AasI GACNNNNNNGTC 1 cut(s) 495
AatII GACGTC 4 cut(s) 216, 276, 299, 462
Acc36I ACCTGC 1 cut(s) 530
AccB1I GGYRCC 1 cut(s) 45
AccI GTMKAC 3 cut(s) 240, 270, 462
AclWI GGATC 1 cut(s) 184
AcuI CTGAAG 1 cut(s) 91
AcvI CACGTG 2 cut(s) 52, 379
AcyI GRCGYC 4 cut(s) 213, 273, 296, 459
AfaI GTAC 1 cut(s) 383
AflIII ACRYGT 2 cut(s) 51, 378
AgsI TTSAA 3 cut(s) 306, 494, 518
AjnI CCWGG 1 cut(s) 117
AjuI GAANNNNNNNTTGG 2 cut(s) 474, 506
AluBI AGCT 4 cut(s) 34, 124, 364, 448
AluI AGCT 4 cut(s) 34, 124, 364, 448
Alw21I GWGCWC 1 cut(s) 126
AlwI GGATC 1 cut(s) 184
AoxI GGCC 1 cut(s) 261
ApeKI GCWGC 2 cut(s) 361, 372
AspLEI GCGC 1 cut(s) 320
BanI GGYRCC 1 cut(s) 45
BanII GRGCYC 1 cut(s) 126
BbrPI CACGTG 2 cut(s) 52, 379
BbsI GAAGAC 1 cut(s) 506
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 373, 384
BccI CCATC 3 cut(s) 7, 204, 393
BciT130I CCWGG 1 cut(s) 119
BclI TGATCA 1 cut(s) 28
BfuAI ACCTGC 1 cut(s) 530
BisI GCNGC 5 cut(s) 184, 207, 362, 373, 439
BlpI GCTNAGC 1 cut(s) 357
BlsI GCNGC 5 cut(s) 185, 208, 363, 374, 440
Bme1390I CCNGG 1 cut(s) 119
BmiI GGNNCC 1 cut(s) 47
BmrFI CCNGG 1 cut(s) 119
BmsI GCATC 2 cut(s) 337, 405
BoxI GACNNNNGTC 1 cut(s) 86
BpiI GAAGAC 1 cut(s) 506
BplI GAGNNNNNCTC 2 cut(s) 55, 87
BpmI CTGGAG 3 cut(s) 132, 140, 456
Bpu1102I GCTNAGC 1 cut(s) 357
BsaAI YACGTR 3 cut(s) 52, 268, 379
BsaHI GRCGYC 4 cut(s) 213, 273, 296, 459
BsaJI CCNNGG 2 cut(s) 258, 404
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
Bse118I RCCGGY 2 cut(s) 76, 250
BseBI CCWGG 1 cut(s) 119
BseDI CCNNGG 2 cut(s) 258, 404
BseGI GGATG 4 cut(s) 276, 352, 385, 396
BseRI GAGGAG 1 cut(s) 167
BseXI GCAGC 2 cut(s) 373, 384
Bsh1285I CGRYCG 2 cut(s) 87, 285
BshFI GGCC 1 cut(s) 263
BshNI GGYRCC 1 cut(s) 45
BsiEI CGRYCG 2 cut(s) 87, 285
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 2 cut(s) 77, 251
BsnI GGCC 1 cut(s) 263
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 2 cut(s) 28, 189
Bsp1720I GCTNAGC 1 cut(s) 357
BspANI GGCC 1 cut(s) 263
BspHI TCATGA 1 cut(s) 25
BspLI GGNNCC 1 cut(s) 47
BspMI ACCTGC 1 cut(s) 530
BspPI GGATC 1 cut(s) 184
BspT107I GGYRCC 1 cut(s) 45
BsrFI RCCGGY 2 cut(s) 76, 250
BssAI RCCGGY 2 cut(s) 76, 250
BssECI CCNNGG 2 cut(s) 258, 404
BssMI GATC 2 cut(s) 28, 189
BssNI GRCGYC 4 cut(s) 213, 273, 296, 459
BssT1I CCWWGG 2 cut(s) 258, 404
Bst2UI CCWGG 1 cut(s) 119
Bst4CI ACNGT 5 cut(s) 88, 286, 313, 512, 552
BstACI GRCGYC 4 cut(s) 213, 273, 296, 459
BstAPI GCANNNNNTGC 1 cut(s) 426
BstBAI YACGTR 3 cut(s) 52, 268, 379
BstC8I GCNNGC 5 cut(s) 78, 231, 252, 366, 370
BstDEI CTNAG 1 cut(s) 357
BstF5I GGATG 4 cut(s) 276, 352, 385, 396
BstHHI GCGC 1 cut(s) 320
BstKTI GATC 2 cut(s) 31, 192
BstMBI GATC 2 cut(s) 28, 189
BstMCI CGRYCG 2 cut(s) 87, 285
BstMWI GCNNNNNNNGC 8 cut(s) 54, 63, 152, 168, 260, 347, 426, 435
BstNI CCWGG 1 cut(s) 119
BstPAI GACNNNNGTC 1 cut(s) 86
BstSCI CCNGG 1 cut(s) 117
BstSNI TACGTA 1 cut(s) 268
BstV1I GCAGC 2 cut(s) 373, 384
BstV2I GAAGAC 1 cut(s) 506
BsuRI GGCC 1 cut(s) 263
BtsCI GGATG 4 cut(s) 276, 352, 385, 396
BtsI GCAGTG 1 cut(s) 100
BtsIMutI CAGTG 1 cut(s) 100
BveI ACCTGC 1 cut(s) 530
Cac8I GCNNGC 5 cut(s) 78, 231, 252, 366, 370
CciI TCATGA 1 cut(s) 25
CfoI GCGC 1 cut(s) 320
Cfr10I RCCGGY 2 cut(s) 76, 250
Csp6I GTAC 1 cut(s) 382
CspCI CAANNNNNGTGG 1 cut(s) 523
CviAII CATG 5 cut(s) 26, 61, 127, 143, 426
CviQI GTAC 1 cut(s) 382
DdeI CTNAG 1 cut(s) 357
DpnI GATC 2 cut(s) 30, 191
DpnII GATC 2 cut(s) 28, 189
DrdI GACNNNNNNGTC 1 cut(s) 495
DseDI GACNNNNNNGTC 1 cut(s) 495
EciI GGCGGA 3 cut(s) 170, 193, 210
Ecl136II GAGCTC 1 cut(s) 124
Eco105I TACGTA 1 cut(s) 268
Eco130I CCWWGG 2 cut(s) 258, 404
Eco147I AGGCCT 1 cut(s) 263
Eco24I GRGCYC 1 cut(s) 126
Eco53kI GAGCTC 1 cut(s) 124
Eco57I CTGAAG 1 cut(s) 91
Eco72I CACGTG 2 cut(s) 52, 379
EcoICRI GAGCTC 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 117
EcoT14I CCWWGG 2 cut(s) 258, 404
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 2 cut(s) 258, 404
FaeI CATG 5 cut(s) 29, 64, 130, 146, 429
FaiI YATR 5 cut(s) 27, 62, 128, 144, 427
FatI CATG 5 cut(s) 25, 60, 126, 142, 425
FauI CCCGC 3 cut(s) 234, 238, 425
FbaI TGATCA 1 cut(s) 28
FblI GTMKAC 3 cut(s) 240, 270, 462
Fnu4HI GCNGC 5 cut(s) 184, 207, 362, 373, 439
FokI GGATG 4 cut(s) 263, 359, 372, 383
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 5 cut(s) 184, 207, 362, 373, 439
GlaI GCGC 1 cut(s) 319
GluI GCNGC 5 cut(s) 184, 207, 362, 373, 439
GsuI CTGGAG 3 cut(s) 132, 140, 456
HaeIII GGCC 1 cut(s) 263
HapII CCGG 2 cut(s) 77, 251
HhaI GCGC 1 cut(s) 320
Hin1I GRCGYC 4 cut(s) 213, 273, 296, 459
Hin1II CATG 5 cut(s) 29, 64, 130, 146, 429
Hin6I GCGC 1 cut(s) 318
HinP1I GCGC 1 cut(s) 318
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HinfI GANTC 2 cut(s) 70, 476
HpaII CCGG 2 cut(s) 77, 251
Hpy166II GTNNAC 5 cut(s) 98, 241, 271, 382, 463
Hpy188III TCNNGA 5 cut(s) 26, 149, 280, 389, 473
Hpy8I GTNNAC 5 cut(s) 98, 241, 271, 382, 463
Hpy99I CGWCG 5 cut(s) 85, 92, 215, 461, 464
HpyAV CCTTC 1 cut(s) 227
HpyCH4III ACNGT 5 cut(s) 88, 286, 313, 512, 552
HpyCH4V TGCA 2 cut(s) 372, 396
HpyF10VI GCNNNNNNNGC 8 cut(s) 54, 63, 152, 168, 260, 347, 426, 435
HpyF3I CTNAG 1 cut(s) 357
Hsp92I GRCGYC 4 cut(s) 213, 273, 296, 459
Hsp92II CATG 5 cut(s) 29, 64, 130, 146, 429
HspAI GCGC 1 cut(s) 318
KroI GCCGGC 2 cut(s) 76, 250
KroNI GCCGGC 2 cut(s) 78, 252
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 2 cut(s) 28, 189
LmnI GCTCC 4 cut(s) 121, 151, 331, 453
Lsp1109I GCAGC 2 cut(s) 373, 384
LweI GCATC 2 cut(s) 337, 405
MaeIII GTNAC 3 cut(s) 67, 297, 546
MalI GATC 2 cut(s) 30, 191
MboI GATC 2 cut(s) 28, 189
MboII GAAGA 3 cut(s) 301, 405, 506
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 1 cut(s) 335
MlyI GAGTC 2 cut(s) 64, 470
MnlI CCTC 8 cut(s) 125, 173, 188, 337, 346, 410, 443, 480
MroNI GCCGGC 2 cut(s) 76, 250
MslI CAYNNNNRTG 1 cut(s) 65
MspA1I CMGCKG 2 cut(s) 34, 364
MspI CCGG 2 cut(s) 77, 251
MspR9I CCNGG 1 cut(s) 119
MvaI CCWGG 1 cut(s) 119
MwoI GCNNNNNNNGC 8 cut(s) 54, 63, 152, 168, 260, 347, 426, 435
NaeI GCCGGC 2 cut(s) 78, 252
NdeII GATC 2 cut(s) 28, 189
NgoMIV GCCGGC 2 cut(s) 76, 250
NlaIII CATG 5 cut(s) 29, 64, 130, 146, 429
NlaIV GGNNCC 1 cut(s) 47
NmuCI GTSAC 2 cut(s) 67, 297
PagI TCATGA 1 cut(s) 25
PaqCI CACCTGC 1 cut(s) 530
PceI AGGCCT 1 cut(s) 263
PdiI GCCGGC 2 cut(s) 78, 252
PfoI TCCNGGA 1 cut(s) 117
PkrI GCNGC 5 cut(s) 185, 208, 363, 374, 440
PleI GAGTC 2 cut(s) 64, 470
PmaCI CACGTG 2 cut(s) 52, 379
PmlI CACGTG 2 cut(s) 52, 379
PpsI GAGTC 2 cut(s) 64, 470
Ppu21I YACGTR 3 cut(s) 52, 268, 379
PshAI GACNNNNGTC 1 cut(s) 86
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 117
PspCI CACGTG 2 cut(s) 52, 379
PspGI CCWGG 1 cut(s) 117
PspN4I GGNNCC 1 cut(s) 47
PvuII CAGCTG 2 cut(s) 34, 364
RsaI GTAC 1 cut(s) 383
RsaNI GTAC 1 cut(s) 382
RseI CAYNNNNRTG 1 cut(s) 65
SacI GAGCTC 1 cut(s) 126
SalI GTCGAC 1 cut(s) 461
SatI GCNGC 5 cut(s) 184, 207, 362, 373, 439
Sau3AI GATC 2 cut(s) 28, 189
SchI GAGTC 2 cut(s) 64, 470
ScrFI CCNGG 1 cut(s) 119
SduI GDGCHC 1 cut(s) 126
SfaNI GCATC 2 cut(s) 337, 405
SmiMI CAYNNNNRTG 1 cut(s) 65
SnaBI TACGTA 1 cut(s) 268
Sse9I AATT 1 cut(s) 335
SseBI AGGCCT 1 cut(s) 263
SstI GAGCTC 1 cut(s) 126
StuI AGGCCT 1 cut(s) 263
StyD4I CCNGG 1 cut(s) 117
StyI CCWWGG 2 cut(s) 258, 404
TaaI ACNGT 5 cut(s) 88, 286, 313, 512, 552
TaqI TCGA 1 cut(s) 462
TasI AATT 1 cut(s) 335
TauI GCSGC 3 cut(s) 186, 209, 441
TscAI CASTG 1 cut(s) 107
TseFI GTSAC 2 cut(s) 67, 297
TseI GCWGC 2 cut(s) 361, 372
Tsp45I GTSAC 2 cut(s) 67, 297
TspDTI ATGAA 2 cut(s) 14, 414
TspRI CASTG 1 cut(s) 107
XmiI GTMKAC 3 cut(s) 240, 270, 462
ZraI GACGTC 4 cut(s) 214, 274, 297, 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.