RLG00000030812

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0013104
Physical Location & Seq
Reverse (-)
67972364 .. 67974437
2074 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030812

Sequence Viewer

Length: 627 bp
ATGGAGATTGGTCATCAGCAGACGTTCTGGTGCCACGACTGCGACATGAGCGTGAGTCTGCCGACGACGACGACGACGACGAGTGTTGTCTGTCCTCACTGCTTCAGCGACCTCCTGGAGCTGATGGATTCGAGTAGTGGCGACAATTTCCAAGCTCCAGAAGCATTCCGCCGCCCCCACGAGGACAACTACCGCCTCAACAGCCCAGTCCTCCAACGGCTGATCCACCGCCTCTCCGACTCCTCCGAGGACGACGTCGTCGTTCCGCCGCCCCCCTCCAATATCTACTTACTGCCGGCTTCCAAGGCGTACGTAGCCGTCATCCCAACGGTGAGGATGACGTCACGTTCAATGTCGGTGTGCGCTGTTTGTAAGGAGCAATTTGCGGAGGATGCGGAGGCTAAGCAGCTGGCTTGCAACCACGTGTACCATCCTGACTGCATTCTCCCTTGGCTTTCTTCCCGAAGTTCATGCCCACTCTGCCGCTATCAGCTCCCCACCGACGACCACCACACTCCTCCTCAACAAGACTCCCAACACCGAATTCAACGTCTTGTGCATTACCGACTTCAACTTCAGCTCCAGCTCCAGCTCGACCGCTCATCCTTTAATGGTGGTTTACAGTAG

Protein Analysis

209

Amino Acids

23.62

Weight (kDa)

5.82

Isoelectric Point (pI)

80.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 7 - 42 1.9e-10 zinc-ribbon
zf-rbx1 PF12678 117 - 162 1e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 121 - 162 3.3e-12 Ring finger domain
zf-C3HC4_2 PF13923 121 - 161 3.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 121 - 161 1.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 121 - 148 3.1e-06 RING-like zinc finger
zf-RING_UBOX PF13445 121 - 159 9e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013104)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44330 AT3G60080
fragaria_vesca FvH4_7g00020 FvH4_7g00020
malus_domestica MD02G1319700.v1.1
prunus_persica Prupe.2G002400_v2.0.a1 Prupe.8G211400_v2.0.a1
pyrus_communis pycom02g26770
rosa_chinensis RchiOBHm_Chr1g0313221
rosa_laevigata RLG00000030812
rosa_multiflora Rmu_sc0013586.1_g000001 Rmu_sc0027690.1_g000001
rosa_roxburghii Rroxscaffold_4G00332330
rosa_rugosa RorugPtG0001800.1
rosa_samantha Rh1AG001800 Rh1CG004000 Rh1DG001700
rosa_wichuraiana Rw1G000090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 257
AatII GACGTC 2 cut(s) 258, 344
AccB1I GGYRCC 1 cut(s) 30
AccBSI CCGCTC 1 cut(s) 600
AclWI GGATC 1 cut(s) 217
AcsI RAATTY 1 cut(s) 543
AcuI CTGAAG 2 cut(s) 88, 560
AcvI CACGTG 1 cut(s) 424
AcyI GRCGYC 2 cut(s) 255, 341
AfaI GTAC 2 cut(s) 311, 428
AfiI CCNNNNNNNGG 1 cut(s) 181
AflIII ACRYGT 1 cut(s) 423
AgsI TTSAA 3 cut(s) 351, 548, 572
AjnI CCWGG 1 cut(s) 114
AjuI GAANNNNNNNTTGG 2 cut(s) 528, 560
AluBI AGCT 7 cut(s) 121, 155, 409, 493, 580, 586, 592
AluI AGCT 7 cut(s) 121, 155, 409, 493, 580, 586, 592
AlwI GGATC 1 cut(s) 217
ApeKI GCWGC 1 cut(s) 406
ApoI RAATTY 1 cut(s) 543
ArsI GACNNNNNNTTYG 2 cut(s) 535, 567
AspLEI GCGC 1 cut(s) 365
AsuHPI GGTGA 1 cut(s) 343
BanI GGYRCC 1 cut(s) 30
BauI CACGAG 1 cut(s) 179
BbrPI CACGTG 1 cut(s) 424
BbvI GCAGC 1 cut(s) 418
BccI CCATC 2 cut(s) 118, 438
BceAI ACGGC 2 cut(s) 233, 302
BcgI CGANNNNNNTGC 2 cut(s) 453, 487
BciT130I CCWGG 1 cut(s) 116
BisI GCNGC 4 cut(s) 172, 269, 407, 484
BlpI GCTNAGC 1 cut(s) 402
BlsI GCNGC 4 cut(s) 173, 270, 408, 485
Bme1390I CCNGG 1 cut(s) 116
BmiI GGNNCC 1 cut(s) 32
BmrFI CCNGG 1 cut(s) 116
BmrI ACTGGG 1 cut(s) 200
BmsI GCATC 1 cut(s) 382
BmuI ACTGGG 1 cut(s) 200
BpmI CTGGAG 4 cut(s) 137, 141, 566, 572
Bpu1102I GCTNAGC 1 cut(s) 402
BsaAI YACGTR 2 cut(s) 313, 424
BsaHI GRCGYC 2 cut(s) 255, 341
BsaJI CCNNGG 3 cut(s) 246, 303, 449
BsaXI ACNNNNNCTCC 4 cut(s) 218, 248, 564, 594
Bsc4I CCNNNNNNNGG 1 cut(s) 181
Bse118I RCCGGY 1 cut(s) 295
Bse1I ACTGG 1 cut(s) 206
BseBI CCWGG 1 cut(s) 116
BseDI CCNNGG 3 cut(s) 246, 303, 449
BseGI GGATG 5 cut(s) 321, 342, 397, 430, 602
BseLI CCNNNNNNNGG 1 cut(s) 181
BseNI ACTGG 1 cut(s) 206
BseRI GAGGAG 3 cut(s) 232, 507, 510
BseXI GCAGC 1 cut(s) 418
Bsh1285I CGRYCG 1 cut(s) 598
BshNI GGYRCC 1 cut(s) 30
BsiEI CGRYCG 1 cut(s) 598
BsiSI CCGG 1 cut(s) 296
BsiWI CGTACG 1 cut(s) 309
BslI CCNNNNNNNGG 1 cut(s) 181
BsmI GAATGC 2 cut(s) 164, 441
Bsp143I GATC 1 cut(s) 222
Bsp1720I GCTNAGC 1 cut(s) 402
BspLI GGNNCC 1 cut(s) 32
BspPI GGATC 1 cut(s) 217
BspT107I GGYRCC 1 cut(s) 30
BsrBI CCGCTC 1 cut(s) 600
BsrFI RCCGGY 1 cut(s) 295
BsrI ACTGG 1 cut(s) 206
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 3 cut(s) 246, 303, 449
BssMI GATC 1 cut(s) 222
BssNI GRCGYC 2 cut(s) 255, 341
BssSI CACGAG 1 cut(s) 179
BssT1I CCWWGG 2 cut(s) 303, 449
Bst2BI CACGAG 1 cut(s) 179
Bst2UI CCWGG 1 cut(s) 116
Bst4CI ACNGT 2 cut(s) 331, 624
BstACI GRCGYC 2 cut(s) 255, 341
BstBAI YACGTR 2 cut(s) 313, 424
BstC8I GCNNGC 3 cut(s) 297, 411, 415
BstDEI CTNAG 1 cut(s) 402
BstF5I GGATG 5 cut(s) 321, 342, 397, 430, 602
BstHHI GCGC 1 cut(s) 365
BstKTI GATC 1 cut(s) 225
BstMBI GATC 1 cut(s) 222
BstMCI CGRYCG 1 cut(s) 598
BstMWI GCNNNNNNNGC 8 cut(s) 39, 48, 161, 201, 305, 314, 392, 480
BstNI CCWGG 1 cut(s) 116
BstSCI CCNGG 1 cut(s) 114
BstSNI TACGTA 1 cut(s) 313
BstV1I GCAGC 1 cut(s) 418
BtsCI GGATG 5 cut(s) 321, 342, 397, 430, 602
BtsI GCAGTG 1 cut(s) 97
BtsIMutI CAGTG 1 cut(s) 97
Cac8I GCNNGC 3 cut(s) 297, 411, 415
CfoI GCGC 1 cut(s) 365
Cfr10I RCCGGY 1 cut(s) 295
Csp6I GTAC 2 cut(s) 310, 427
CviAII CATG 2 cut(s) 46, 471
CviQI GTAC 2 cut(s) 310, 427
DdeI CTNAG 1 cut(s) 402
DpnI GATC 1 cut(s) 224
DpnII GATC 1 cut(s) 222
DrdI GACNNNNNNGTC 1 cut(s) 257
DseDI GACNNNNNNGTC 1 cut(s) 257
EciI GGCGGA 2 cut(s) 158, 255
Eco105I TACGTA 1 cut(s) 313
Eco130I CCWWGG 2 cut(s) 303, 449
Eco57I CTGAAG 2 cut(s) 88, 560
Eco72I CACGTG 1 cut(s) 424
EcoRI GAATTC 1 cut(s) 543
EcoRII CCWGG 1 cut(s) 114
EcoT14I CCWWGG 2 cut(s) 303, 449
ErhI CCWWGG 2 cut(s) 303, 449
FaeI CATG 2 cut(s) 49, 474
FaiI YATR 2 cut(s) 47, 472
FatI CATG 2 cut(s) 45, 470
Fnu4HI GCNGC 4 cut(s) 172, 269, 407, 484
FokI GGATG 5 cut(s) 308, 349, 404, 417, 589
Fsp4HI GCNGC 4 cut(s) 172, 269, 407, 484
GlaI GCGC 1 cut(s) 364
GluI GCNGC 4 cut(s) 172, 269, 407, 484
GsuI CTGGAG 4 cut(s) 137, 141, 566, 572
HapII CCGG 1 cut(s) 296
HhaI GCGC 1 cut(s) 365
Hin1I GRCGYC 2 cut(s) 255, 341
Hin1II CATG 2 cut(s) 49, 474
Hin6I GCGC 1 cut(s) 363
HinP1I GCGC 1 cut(s) 363
HinfI GANTC 4 cut(s) 55, 128, 239, 530
HpaII CCGG 1 cut(s) 296
HphI GGTGA 1 cut(s) 343
Hpy166II GTNNAC 2 cut(s) 427, 620
Hpy188I TCNGA 2 cut(s) 238, 247
Hpy188III TCNNGA 3 cut(s) 158, 434, 462
Hpy8I GTNNAC 2 cut(s) 427, 620
HpyCH4III ACNGT 2 cut(s) 331, 624
HpyCH4IV ACGT 7 cut(s) 23, 255, 312, 341, 346, 423, 550
HpyCH4V TGCA 3 cut(s) 417, 441, 559
HpyF10VI GCNNNNNNNGC 8 cut(s) 39, 48, 161, 201, 305, 314, 392, 480
HpyF3I CTNAG 1 cut(s) 402
HpySE526I ACGT 7 cut(s) 23, 255, 312, 341, 346, 423, 550
Hsp92I GRCGYC 2 cut(s) 255, 341
Hsp92II CATG 2 cut(s) 49, 474
HspAI GCGC 1 cut(s) 363
KroI GCCGGC 1 cut(s) 295
KroNI GCCGGC 1 cut(s) 297
Kzo9I GATC 1 cut(s) 222
LmnI GCTCC 6 cut(s) 118, 160, 376, 498, 585, 591
Lsp1109I GCAGC 1 cut(s) 418
LweI GCATC 1 cut(s) 382
MaeII ACGT 7 cut(s) 23, 255, 312, 341, 346, 423, 550
MaeIII GTNAC 1 cut(s) 342
MalI GATC 1 cut(s) 224
MbiI CCGCTC 1 cut(s) 600
MboI GATC 1 cut(s) 222
MboII GAAGA 1 cut(s) 450
MluCI AATT 3 cut(s) 145, 380, 543
MlyI GAGTC 3 cut(s) 64, 233, 524
MmeI TCCRAC 2 cut(s) 238, 261
MroNI GCCGGC 1 cut(s) 295
MseI TTAA 1 cut(s) 609
MslI CAYNNNNRTG 1 cut(s) 50
MspA1I CMGCKG 1 cut(s) 409
MspI CCGG 1 cut(s) 296
MspR9I CCNGG 1 cut(s) 116
Mva1269I GAATGC 2 cut(s) 164, 441
MvaI CCWGG 1 cut(s) 116
MwoI GCNNNNNNNGC 8 cut(s) 39, 48, 161, 201, 305, 314, 392, 480
NaeI GCCGGC 1 cut(s) 297
NdeII GATC 1 cut(s) 222
NgoMIV GCCGGC 1 cut(s) 295
NlaIII CATG 2 cut(s) 49, 474
NlaIV GGNNCC 1 cut(s) 32
NmuCI GTSAC 1 cut(s) 342
PcsI WCGNNNNNNNCGW 4 cut(s) 71, 74, 77, 258
PctI GAATGC 2 cut(s) 164, 441
PdiI GCCGGC 1 cut(s) 297
PfeI GAWTC 1 cut(s) 128
Pfl23II CGTACG 1 cut(s) 309
PflFI GACNNNGTC 2 cut(s) 254, 257
PfoI TCCNGGA 1 cut(s) 114
PkrI GCNGC 4 cut(s) 173, 270, 408, 485
PleI GAGTC 3 cut(s) 63, 233, 524
PmaCI CACGTG 1 cut(s) 424
PmlI CACGTG 1 cut(s) 424
PpsI GAGTC 3 cut(s) 63, 233, 524
Ppu21I YACGTR 2 cut(s) 313, 424
Psp6I CCWGG 1 cut(s) 114
PspCI CACGTG 1 cut(s) 424
PspGI CCWGG 1 cut(s) 114
PspLI CGTACG 1 cut(s) 309
PspN4I GGNNCC 1 cut(s) 32
PsyI GACNNNGTC 2 cut(s) 254, 257
PvuII CAGCTG 1 cut(s) 409
RsaI GTAC 2 cut(s) 311, 428
RsaNI GTAC 2 cut(s) 310, 427
RseI CAYNNNNRTG 1 cut(s) 50
SaqAI TTAA 1 cut(s) 609
SatI GCNGC 4 cut(s) 172, 269, 407, 484
Sau3AI GATC 1 cut(s) 222
SchI GAGTC 3 cut(s) 64, 233, 524
ScrFI CCNGG 1 cut(s) 116
SfaNI GCATC 1 cut(s) 382
SmiMI CAYNNNNRTG 1 cut(s) 50
SnaBI TACGTA 1 cut(s) 313
Sse9I AATT 3 cut(s) 145, 380, 543
StyD4I CCNGG 1 cut(s) 114
StyI CCWWGG 2 cut(s) 303, 449
TaaI ACNGT 2 cut(s) 331, 624
TaiI ACGT 7 cut(s) 26, 258, 315, 344, 349, 426, 553
TaqI TCGA 2 cut(s) 131, 594
TasI AATT 3 cut(s) 145, 380, 543
TauI GCSGC 3 cut(s) 174, 271, 486
TfiI GAWTC 1 cut(s) 128
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TscAI CASTG 1 cut(s) 104
TseFI GTSAC 1 cut(s) 342
TseI GCWGC 1 cut(s) 406
Tsp45I GTSAC 1 cut(s) 342
TspDTI ATGAA 1 cut(s) 459
TspRI CASTG 1 cut(s) 104
Tth111I GACNNNGTC 2 cut(s) 254, 257
XapI RAATTY 1 cut(s) 543
ZraI GACGTC 2 cut(s) 256, 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.