FvH4_7g06121

GDSL esterase lipase At1g71250-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0014790
Physical Location & Seq
Reverse (-)
6280332 .. 6281374
1043 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g06121.t1

Sequence Viewer

Length: 972 bp
ATGACAAAGATGAAAGAAACCCTCTTTTCCAAAACTAAAGGCTTGCTTCTTCTTCATCATCTAGCTCCATTGATCATGGGGTTTATGATCTTAGCTGATGCAAAAGTGAACGAGGTTTCAAGTTTCCCAAATTGTAGAAATGGCTCTGCAGCATTCTACGTATTGGGAGATTCGTCTGTTGATTGCGGAGACAACACACTGTTTTACCCACTCATTCACAGTTACCTGTCCTTGTATTCGTGTAATGGTTCCAACGGCTCAACTCTTATTCCTCATCTTCTTGCTGAGAAGATGGGCATGCAGAACATCTTACCATTCTACAGTCAAAATGGATCCATTAATGGAATACAGAGAGGCCTTAACTTCGGTTCAGCACACGCAACAATCATGAATCACGGCAGCCAGAGTTATCAGTCTGTGAACCAGCAGCTCCGGCAAGTTTTTGATTCTCTGCAACTCTTACAACTGCAGCTTAGCGAGGACAGAGCTCTCAGTTTCATCAAATCTTCCATCTTCTACCTCTCCTTTGGCAAAGATGACTTTATAGAACTCCTCCTACAAAATGCTTCGGGTACTGCAAAACAGAACAGTACTGATCAAGAATTTGCACACATTTTAGCAGATCAAATGATACATGTTATACGGAACCTTTATGCCATGAATGTGAGGAGGATCATATGCATGGGAATATTACCTTTGGGATGCACTCCAAGGGTGTTATTGAACCAATACAATTCCACAGCCCAACCACATGATGACGACGATGATGTTGCAAGGGGATGTGTAGAGGAGATCAATGTGCATGTCTTGGAGTACAACAGAGAGCTGCGTGAGCAAATCTCCCAGCTTCATGTAGAGTTGCCTGATGCACAGCTTCTGTTTTGTGATATTTACCAAGGACTTTCAGAGATCATAAACAACCCCCAACAATATGGTACACAAACACAATACTTCAATTTCTTCTGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

324

Amino Acids

36.37

Weight (kDa)

5.49

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 53 - 313 2e-16 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014790)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06121 FvH4_7g06190
malus_domestica MD00G1178200.v1.1
prunus_persica Prupe.6G152900_v2.0.a1
pyrus_communis pycom11g15560
rosa_chinensis RchiOBHm_Chr1g0333821
rosa_laevigata RLG00000029528
rosa_multiflora Rmu_sc0000026.1_g000017 Rmu_sc0007177.1_g000005
rosa_roxburghii Rroxscaffold_4G00317200
rosa_rugosa Rorug01G0112100
rosa_samantha Rh1AG134500 Rh1AG134600 Rh1BG102800 Rh1CG127900 Rh1DG140000
rosa_wichuraiana Rw1G011220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 186
AclWI GGATC 3 cut(s) 327, 340, 680
AcsI RAATTY 1 cut(s) 602
AfaI GTAC 4 cut(s) 574, 592, 815, 937
AflIII ACRYGT 1 cut(s) 634
AgsI TTSAA 3 cut(s) 120, 724, 955
AluBI AGCT 8 cut(s) 65, 95, 430, 472, 488, 826, 847, 874
AluI AGCT 8 cut(s) 65, 95, 430, 472, 488, 826, 847, 874
Alw21I GWGCWC 1 cut(s) 490
Alw26I GTCTC 1 cut(s) 183
AlwI GGATC 3 cut(s) 327, 340, 680
AlwNI CAGNNNCTG 1 cut(s) 877
AoxI GGCC 1 cut(s) 355
ApeKI GCWGC 5 cut(s) 149, 399, 427, 469, 826
ApoI RAATTY 1 cut(s) 602
AseI ATTAAT 1 cut(s) 339
BamHI GGATCC 1 cut(s) 332
BanII GRGCYC 1 cut(s) 490
Bbv12I GWGCWC 1 cut(s) 490
BbvI GCAGC 5 cut(s) 161, 411, 439, 481, 813
BccI CCATC 2 cut(s) 286, 518
BceAI ACGGC 2 cut(s) 271, 412
BcgI CGANNNNNNTGC 2 cut(s) 752, 786
BclI TGATCA 2 cut(s) 72, 595
BcoDI GTCTC 1 cut(s) 183
BfaI CTAG 1 cut(s) 62
BfmI CTRYAG 3 cut(s) 147, 319, 467
BisI GCNGC 5 cut(s) 150, 400, 428, 470, 827
BlpI GCTNAGC 1 cut(s) 473
BlsI GCNGC 5 cut(s) 151, 401, 429, 471, 828
BmcAI AGTACT 1 cut(s) 592
BmiI GGNNCC 3 cut(s) 250, 334, 647
BmsI GCATC 3 cut(s) 88, 692, 856
BplI GAGNNNNNCTC 2 cut(s) 824, 856
Bpu1102I GCTNAGC 1 cut(s) 473
BsaAI YACGTR 1 cut(s) 160
BsaJI CCNNGG 2 cut(s) 710, 895
BseDI CCNNGG 2 cut(s) 710, 895
BseGI GGATG 2 cut(s) 707, 785
BseMII CTCAG 2 cut(s) 276, 505
BseRI GAGGAG 3 cut(s) 542, 682, 803
BseXI GCAGC 5 cut(s) 161, 411, 439, 481, 813
BseYI CCCAGC 1 cut(s) 843
BshFI GGCC 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 490
BsiSI CCGG 1 cut(s) 433
BsmAI GTCTC 1 cut(s) 183
BsmI GAATGC 1 cut(s) 152
BsnI GGCC 1 cut(s) 357
Bsp1286I GDGCHC 1 cut(s) 490
Bsp143I GATC 8 cut(s) 72, 87, 332, 595, 622, 672, 792, 909
Bsp1720I GCTNAGC 1 cut(s) 473
BspACI CCGC 1 cut(s) 186
BspANI GGCC 1 cut(s) 357
BspCNI CTCAG 2 cut(s) 277, 504
BspHI TCATGA 1 cut(s) 387
BspLI GGNNCC 3 cut(s) 250, 334, 647
BspMAI CTGCAG 2 cut(s) 151, 471
BspPI GGATC 3 cut(s) 327, 340, 680
BssECI CCNNGG 2 cut(s) 710, 895
BssMI GATC 8 cut(s) 72, 87, 332, 595, 622, 672, 792, 909
BssT1I CCWWGG 2 cut(s) 710, 895
Bst4CI ACNGT 4 cut(s) 201, 221, 323, 590
BstBAI YACGTR 1 cut(s) 160
BstC8I GCNNGC 2 cut(s) 44, 299
BstDEI CTNAG 4 cut(s) 91, 285, 473, 491
BstF5I GGATG 2 cut(s) 707, 785
BstKTI GATC 8 cut(s) 75, 90, 335, 598, 625, 675, 795, 912
BstMAI GTCTC 1 cut(s) 183
BstMBI GATC 8 cut(s) 72, 87, 332, 595, 622, 672, 792, 909
BstMWI GCNNNNNNNGC 2 cut(s) 433, 832
BstNSI RCATGY 3 cut(s) 301, 638, 806
BstSFI CTRYAG 3 cut(s) 147, 319, 467
BstSNI TACGTA 1 cut(s) 160
BstV1I GCAGC 5 cut(s) 161, 411, 439, 481, 813
BstX2I RGATCY 1 cut(s) 332
BstXI CCANNNNNNTGG 1 cut(s) 932
BstYI RGATCY 1 cut(s) 332
BsuRI GGCC 1 cut(s) 357
BtsCI GGATG 2 cut(s) 707, 785
BtsIMutI CAGTG 1 cut(s) 197
Cac8I GCNNGC 2 cut(s) 44, 299
CaiI CAGNNNCTG 1 cut(s) 877
CciI TCATGA 1 cut(s) 387
Csp6I GTAC 4 cut(s) 573, 591, 814, 936
CviAII CATG 9 cut(s) 76, 298, 388, 635, 658, 682, 752, 803, 851
CviQI GTAC 4 cut(s) 573, 591, 814, 936
DdeI CTNAG 4 cut(s) 91, 285, 473, 491
DpnI GATC 8 cut(s) 74, 89, 334, 597, 624, 674, 794, 911
DpnII GATC 8 cut(s) 72, 87, 332, 595, 622, 672, 792, 909
Ecl136II GAGCTC 1 cut(s) 488
Eco105I TACGTA 1 cut(s) 160
Eco130I CCWWGG 2 cut(s) 710, 895
Eco147I AGGCCT 1 cut(s) 357
Eco24I GRGCYC 1 cut(s) 490
Eco53kI GAGCTC 1 cut(s) 488
EcoICRI GAGCTC 1 cut(s) 488
EcoT14I CCWWGG 2 cut(s) 710, 895
EcoT22I ATGCAT 1 cut(s) 683
EcoT38I GRGCYC 1 cut(s) 490
ErhI CCWWGG 2 cut(s) 710, 895
FaeI CATG 9 cut(s) 79, 301, 391, 638, 661, 685, 755, 806, 854
FalI AAGNNNNNCTT 2 cut(s) 30, 62
FatI CATG 9 cut(s) 75, 297, 387, 634, 657, 681, 751, 802, 850
FauNDI CATATG 1 cut(s) 677
FbaI TGATCA 2 cut(s) 72, 595
Fnu4HI GCNGC 5 cut(s) 150, 400, 428, 470, 827
FokI GGATG 2 cut(s) 714, 792
FriOI GRGCYC 1 cut(s) 490
Fsp4HI GCNGC 5 cut(s) 150, 400, 428, 470, 827
FspBI CTAG 1 cut(s) 62
GluI GCNGC 5 cut(s) 150, 400, 428, 470, 827
GsaI CCCAGC 1 cut(s) 847
HaeIII GGCC 1 cut(s) 357
HapII CCGG 1 cut(s) 433
Hin1II CATG 9 cut(s) 79, 301, 391, 638, 661, 685, 755, 806, 854
HinfI GANTC 3 cut(s) 170, 391, 446
HpaII CCGG 1 cut(s) 433
Hpy166II GTNNAC 3 cut(s) 109, 421, 938
Hpy188I TCNGA 1 cut(s) 907
Hpy188III TCNNGA 2 cut(s) 388, 599
Hpy8I GTNNAC 3 cut(s) 109, 421, 938
Hpy99I CGWCG 1 cut(s) 764
HpyCH4III ACNGT 4 cut(s) 201, 221, 323, 590
HpyCH4IV ACGT 1 cut(s) 159
HpyF10VI GCNNNNNNNGC 2 cut(s) 433, 832
HpyF3I CTNAG 4 cut(s) 91, 285, 473, 491
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 9 cut(s) 79, 301, 391, 638, 661, 685, 755, 806, 854
Ksp22I TGATCA 2 cut(s) 72, 595
Kzo9I GATC 8 cut(s) 72, 87, 332, 595, 622, 672, 792, 909
LmnI GCTCC 2 cut(s) 70, 435
LpnPI CCDG 6 cut(s) 239, 416, 437, 446, 857, 876
Lsp1109I GCAGC 5 cut(s) 161, 411, 439, 481, 813
LweI GCATC 3 cut(s) 88, 692, 856
MaeI CTAG 1 cut(s) 62
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 1 cut(s) 221
MalI GATC 8 cut(s) 74, 89, 334, 597, 624, 674, 794, 911
MboI GATC 8 cut(s) 72, 87, 332, 595, 622, 672, 792, 909
MboII GAAGA 7 cut(s) 41, 44, 269, 301, 498, 505, 952
MflI RGATCY 1 cut(s) 332
MhlI GDGCHC 1 cut(s) 490
MluCI AATT 4 cut(s) 130, 602, 733, 955
MmeI TCCRAC 1 cut(s) 276
Mph1103I ATGCAT 1 cut(s) 683
MseI TTAA 2 cut(s) 339, 360
MslI CAYNNNNRTG 2 cut(s) 662, 680
MspI CCGG 1 cut(s) 433
Mva1269I GAATGC 1 cut(s) 152
MwoI GCNNNNNNNGC 2 cut(s) 433, 832
NdeI CATATG 1 cut(s) 677
NdeII GATC 8 cut(s) 72, 87, 332, 595, 622, 672, 792, 909
NlaIII CATG 9 cut(s) 79, 301, 391, 638, 661, 685, 755, 806, 854
NlaIV GGNNCC 3 cut(s) 250, 334, 647
NsiI ATGCAT 1 cut(s) 683
NspI RCATGY 3 cut(s) 301, 638, 806
PaeI GCATGC 1 cut(s) 301
PagI TCATGA 1 cut(s) 387
PceI AGGCCT 1 cut(s) 357
PciI ACATGT 1 cut(s) 634
PctI GAATGC 1 cut(s) 152
PfeI GAWTC 3 cut(s) 170, 391, 446
PkrI GCNGC 5 cut(s) 151, 401, 429, 471, 828
Ppu21I YACGTR 1 cut(s) 160
PscI ACATGT 1 cut(s) 634
PshBI ATTAAT 1 cut(s) 339
Psp124BI GAGCTC 1 cut(s) 490
PspFI CCCAGC 1 cut(s) 843
PspN4I GGNNCC 3 cut(s) 250, 334, 647
PsrI GAACNNNNNNTAC 2 cut(s) 540, 572
PstI CTGCAG 2 cut(s) 151, 471
PstNI CAGNNNCTG 1 cut(s) 877
PsuI RGATCY 1 cut(s) 332
RsaI GTAC 4 cut(s) 574, 592, 815, 937
RsaNI GTAC 4 cut(s) 573, 591, 814, 936
RseI CAYNNNNRTG 2 cut(s) 662, 680
SacI GAGCTC 1 cut(s) 490
SaqAI TTAA 2 cut(s) 339, 360
SatI GCNGC 5 cut(s) 150, 400, 428, 470, 827
Sau3AI GATC 8 cut(s) 72, 87, 332, 595, 622, 672, 792, 909
ScaI AGTACT 1 cut(s) 592
SduI GDGCHC 1 cut(s) 490
SfaNI GCATC 3 cut(s) 88, 692, 856
SfcI CTRYAG 3 cut(s) 147, 319, 467
SmiMI CAYNNNNRTG 2 cut(s) 662, 680
SnaBI TACGTA 1 cut(s) 160
SphI GCATGC 1 cut(s) 301
Sse9I AATT 4 cut(s) 130, 602, 733, 955
SseBI AGGCCT 1 cut(s) 357
SsiI CCGC 1 cut(s) 186
SspI AATATT 1 cut(s) 690
SspMI CTAG 1 cut(s) 62
SstI GAGCTC 1 cut(s) 490
StuI AGGCCT 1 cut(s) 357
StyI CCWWGG 2 cut(s) 710, 895
TaaI ACNGT 4 cut(s) 201, 221, 323, 590
TaiI ACGT 1 cut(s) 162
TasI AATT 4 cut(s) 130, 602, 733, 955
TatI WGTACW 2 cut(s) 590, 813
TfiI GAWTC 3 cut(s) 170, 391, 446
Tru1I TTAA 2 cut(s) 339, 360
Tru9I TTAA 2 cut(s) 339, 360
TscAI CASTG 1 cut(s) 204
TseI GCWGC 5 cut(s) 149, 399, 427, 469, 826
TspDTI ATGAA 6 cut(s) 26, 44, 404, 487, 674, 839
TspGWI ACGGA 1 cut(s) 658
TspRI CASTG 1 cut(s) 204
VspI ATTAAT 1 cut(s) 339
XapI RAATTY 1 cut(s) 602
XceI RCATGY 3 cut(s) 301, 638, 806
XspI CTAG 1 cut(s) 62
ZrmI AGTACT 1 cut(s) 592
Zsp2I ATGCAT 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.