Rh1DG140000

GDSL esterase lipase At1g71250-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0014790
Physical Location & Seq
Reverse (-)
29641896 .. 29647695
5800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG140000.1

Sequence Viewer

Length: 1098 bp
ATGAACCATCAATCATACTTGTTACGCCTATCGCCTAACCGATACACACAAACACCATGCACTGAAACTCAAATGCTGAACGCAACATTAGTATCAAGCAGTGAAATGCAAGCCACACAACAGAACCAAAGTAAGATGACAAAGATGAAAGAAACCCTCTCTTCCAAAACAAAGGGTTTGCTTCTCGTTCATCATCTAGCTCTATTGGTCATGGGGTTTATGGTTTTAGCTCAAGGGAAACTGAAACAGGCTTCGAGTGTCCAAAATTGTAGAAATGACTCTGCAGCATTCTACATATTGGGGGATTCCTCTGTTGATTGCGGAGACAACACGCTCTTTTACCCTCTCATTCACAGTTATCTGTCCTTGTATTCGTGTAATGGTTCCGACGGCTCAACTCTTATTCCCCATCTTCTTGCTGAGAAGATGGGCATGCCAAACATTTTACCATTCTACAGTCAAAATGGATCCATTAATGGAATACAAAGAGGCCTTAACTTCGGTTCAGCACATGCAACGATCTTGAATCACGGCAGCCAGAGTTATCAGTCTGTGAACCAGCAGCTCCGCCAAGTTTTTGATTCTATGCAACTCTTACAATTGCAGCTTAGCGAGGACAGAGCTCTCAGTTTCATCAAATCTTCCATCTTCTACCTCTCCTTTGGCAAAGATGACTTCATAGAACTCTTCCTACGCAATGCCTCGGGTACTGCAAAACAGAACAATACTGATCAAGAATTCTCACACATTTTAGCAGATCAGATGGTACATGTTATACGGAACCTTTATGACATGAATGTGAGGAGGATCATATGCATGGGAATATTGCCGTTGGGATGCACCCCGCGGGTGTTATTGAACCAGTACAATTCCACAGCCCATCCAAATGAAGATGATGATGATGATGTTCCAAGGGGATGTGTAGAGGAGATTAATGCGCAGGTCTTAGAGTACAACAGAGAGCTGAACGAGCAAATCTCTGAGCTTCATGCAGAGTTGCCTGATGCACAGCTTCTGTTTTGTGATGTTTACCAAGGACTTACAACGATCATAAACAACCCAGAACAATATGGTACACAAACACAAACACTTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

365

Amino Acids

40.93

Weight (kDa)

5.41

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 98 - 351 5.3e-17 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014790)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06121 FvH4_7g06190
malus_domestica MD00G1178200.v1.1
prunus_persica Prupe.6G152900_v2.0.a1
pyrus_communis pycom11g15560
rosa_chinensis RchiOBHm_Chr1g0333821
rosa_laevigata RLG00000029528
rosa_multiflora Rmu_sc0000026.1_g000017 Rmu_sc0007177.1_g000005
rosa_roxburghii Rroxscaffold_4G00317200
rosa_rugosa Rorug01G0112100
rosa_samantha Rh1AG134500 Rh1AG134600 Rh1BG102800 Rh1CG127900 Rh1DG140000
rosa_wichuraiana Rw1G011220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 939
Acc36I ACCTGC 1 cut(s) 931
AccII CGCG 1 cut(s) 847
AciI CCGC 4 cut(s) 321, 568, 845, 847
AclWI GGATC 3 cut(s) 462, 475, 815
AcsI RAATTY 1 cut(s) 737
AfaI GTAC 5 cut(s) 709, 768, 866, 953, 1075
AflIII ACRYGT 1 cut(s) 769
AgsI TTSAA 2 cut(s) 526, 859
AluBI AGCT 8 cut(s) 200, 230, 565, 607, 623, 964, 985, 1012
AluI AGCT 8 cut(s) 200, 230, 565, 607, 623, 964, 985, 1012
Alw21I GWGCWC 1 cut(s) 625
Alw26I GTCTC 1 cut(s) 318
AlwI GGATC 3 cut(s) 462, 475, 815
AlwNI CAGNNNCTG 1 cut(s) 1015
Ama87I CYCGRG 1 cut(s) 703
AoxI GGCC 1 cut(s) 490
ApeKI GCWGC 4 cut(s) 284, 534, 562, 604
ApoI RAATTY 1 cut(s) 737
AseI ATTAAT 2 cut(s) 474, 933
AspLEI GCGC 1 cut(s) 940
AvaI CYCGRG 1 cut(s) 703
BaeI ACNNNNGTAYC 2 cut(s) 75, 108
BamHI GGATCC 1 cut(s) 467
BanII GRGCYC 1 cut(s) 625
Bbv12I GWGCWC 1 cut(s) 625
BbvI GCAGC 4 cut(s) 296, 546, 574, 616
BccI CCATC 6 cut(s) 15, 417, 421, 653, 757, 888
BceAI ACGGC 3 cut(s) 406, 547, 814
BclI TGATCA 1 cut(s) 730
BcoDI GTCTC 1 cut(s) 318
BfaI CTAG 1 cut(s) 197
BfmI CTRYAG 2 cut(s) 282, 454
BfuAI ACCTGC 1 cut(s) 931
BisI GCNGC 4 cut(s) 285, 535, 563, 605
BlpI GCTNAGC 1 cut(s) 608
BlsI GCNGC 4 cut(s) 286, 536, 564, 606
BmeT110I CYCGRG 1 cut(s) 703
BmiI GGNNCC 3 cut(s) 385, 469, 782
BmsI GCATC 2 cut(s) 827, 994
BplI GAGNNNNNCTC 2 cut(s) 962, 994
Bpu1102I GCTNAGC 1 cut(s) 608
BpuEI CTTGAG 1 cut(s) 216
BsaJI CCNNGG 4 cut(s) 702, 845, 911, 1033
Bse1I ACTGG 1 cut(s) 862
Bse3DI GCAATG 1 cut(s) 703
BseDI CCNNGG 4 cut(s) 702, 845, 911, 1033
BseGI GGATG 3 cut(s) 842, 880, 923
BseMI GCAATG 1 cut(s) 703
BseMII CTCAG 3 cut(s) 411, 640, 972
BseNI ACTGG 1 cut(s) 862
BseRI GAGGAG 2 cut(s) 817, 941
BseXI GCAGC 4 cut(s) 296, 546, 574, 616
Bsh1236I CGCG 1 cut(s) 847
BshFI GGCC 1 cut(s) 492
BsiHKAI GWGCWC 1 cut(s) 625
BsiHKCI CYCGRG 1 cut(s) 703
BsmAI GTCTC 1 cut(s) 318
BsmI GAATGC 1 cut(s) 287
BsnI GGCC 1 cut(s) 492
BsoBI CYCGRG 1 cut(s) 703
Bsp1286I GDGCHC 1 cut(s) 625
Bsp143I GATC 6 cut(s) 467, 519, 730, 757, 807, 1047
Bsp1720I GCTNAGC 1 cut(s) 608
BspACI CCGC 4 cut(s) 321, 568, 845, 847
BspANI GGCC 1 cut(s) 492
BspCNI CTCAG 3 cut(s) 412, 639, 973
BspFNI CGCG 1 cut(s) 847
BspLI GGNNCC 3 cut(s) 385, 469, 782
BspMAI CTGCAG 1 cut(s) 286
BspMI ACCTGC 1 cut(s) 931
BspPI GGATC 3 cut(s) 462, 475, 815
BsrDI GCAATG 1 cut(s) 703
BsrI ACTGG 1 cut(s) 862
BssECI CCNNGG 4 cut(s) 702, 845, 911, 1033
BssMI GATC 6 cut(s) 467, 519, 730, 757, 807, 1047
BssT1I CCWWGG 2 cut(s) 911, 1033
Bst4CI ACNGT 2 cut(s) 356, 458
Bst6I CTCTTC 2 cut(s) 166, 692
BstC8I GCNNGC 2 cut(s) 111, 434
BstDEI CTNAG 5 cut(s) 420, 608, 626, 946, 981
BstDSI CCRYGG 1 cut(s) 845
BstF5I GGATG 3 cut(s) 842, 880, 923
BstFNI CGCG 1 cut(s) 847
BstHHI GCGC 1 cut(s) 940
BstKTI GATC 6 cut(s) 470, 522, 733, 760, 810, 1050
BstMAI GTCTC 1 cut(s) 318
BstMBI GATC 6 cut(s) 467, 519, 730, 757, 807, 1047
BstMWI GCNNNNNNNGC 1 cut(s) 970
BstNSI RCATGY 3 cut(s) 436, 515, 773
BstSFI CTRYAG 2 cut(s) 282, 454
BstUI CGCG 1 cut(s) 847
BstV1I GCAGC 4 cut(s) 296, 546, 574, 616
BstX2I RGATCY 1 cut(s) 467
BstYI RGATCY 1 cut(s) 467
BsuRI GGCC 1 cut(s) 492
BtgI CCRYGG 1 cut(s) 845
BtsCI GGATG 3 cut(s) 842, 880, 923
BtsI GCAGTG 1 cut(s) 106
BtsIMutI CAGTG 2 cut(s) 60, 106
BveI ACCTGC 1 cut(s) 931
Cac8I GCNNGC 2 cut(s) 111, 434
CaiI CAGNNNCTG 1 cut(s) 1015
CfoI GCGC 1 cut(s) 940
Cfr42I CCGCGG 1 cut(s) 848
Csp6I GTAC 5 cut(s) 708, 767, 865, 952, 1074
CviAII CATG 8 cut(s) 57, 211, 433, 512, 770, 793, 817, 989
CviQI GTAC 5 cut(s) 708, 767, 865, 952, 1074
DdeI CTNAG 5 cut(s) 420, 608, 626, 946, 981
DpnI GATC 6 cut(s) 469, 521, 732, 759, 809, 1049
DpnII GATC 6 cut(s) 467, 519, 730, 757, 807, 1047
Eam1104I CTCTTC 2 cut(s) 166, 692
EarI CTCTTC 2 cut(s) 166, 692
EciI GGCGGA 1 cut(s) 557
Ecl136II GAGCTC 1 cut(s) 623
Eco130I CCWWGG 2 cut(s) 911, 1033
Eco147I AGGCCT 1 cut(s) 492
Eco24I GRGCYC 1 cut(s) 625
Eco53kI GAGCTC 1 cut(s) 623
Eco88I CYCGRG 1 cut(s) 703
EcoICRI GAGCTC 1 cut(s) 623
EcoRI GAATTC 1 cut(s) 737
EcoT14I CCWWGG 2 cut(s) 911, 1033
EcoT22I ATGCAT 1 cut(s) 818
EcoT38I GRGCYC 1 cut(s) 625
ErhI CCWWGG 2 cut(s) 911, 1033
FaeI CATG 8 cut(s) 60, 214, 436, 515, 773, 796, 820, 992
FatI CATG 8 cut(s) 56, 210, 432, 511, 769, 792, 816, 988
FauI CCCGC 2 cut(s) 840, 852
FauNDI CATATG 1 cut(s) 812
FbaI TGATCA 1 cut(s) 730
Fnu4HI GCNGC 4 cut(s) 285, 535, 563, 605
FokI GGATG 3 cut(s) 849, 867, 930
FriOI GRGCYC 1 cut(s) 625
Fsp4HI GCNGC 4 cut(s) 285, 535, 563, 605
FspBI CTAG 1 cut(s) 197
FspI TGCGCA 1 cut(s) 939
GlaI GCGC 1 cut(s) 939
GluI GCNGC 4 cut(s) 285, 535, 563, 605
HaeIII GGCC 1 cut(s) 492
HhaI GCGC 1 cut(s) 940
Hin1II CATG 8 cut(s) 60, 214, 436, 515, 773, 796, 820, 992
Hin6I GCGC 1 cut(s) 938
HinP1I GCGC 1 cut(s) 938
HinfI GANTC 4 cut(s) 278, 305, 526, 581
Hpy166II GTNNAC 3 cut(s) 556, 1030, 1076
Hpy188I TCNGA 3 cut(s) 388, 762, 982
Hpy188III TCNNGA 2 cut(s) 523, 734
Hpy8I GTNNAC 3 cut(s) 556, 1030, 1076
Hpy99I CGWCG 1 cut(s) 392
HpyCH4III ACNGT 2 cut(s) 356, 458
HpyF10VI GCNNNNNNNGC 1 cut(s) 970
HpyF3I CTNAG 5 cut(s) 420, 608, 626, 946, 981
Hsp92II CATG 8 cut(s) 60, 214, 436, 515, 773, 796, 820, 992
HspAI GCGC 1 cut(s) 938
Ksp22I TGATCA 1 cut(s) 730
KspI CCGCGG 1 cut(s) 848
Kzo9I GATC 6 cut(s) 467, 519, 730, 757, 807, 1047
LmnI GCTCC 1 cut(s) 570
LpnPI CCDG 7 cut(s) 233, 551, 572, 875, 926, 1014, 1074
Lsp1109I GCAGC 4 cut(s) 296, 546, 574, 616
LweI GCATC 2 cut(s) 827, 994
MaeI CTAG 1 cut(s) 197
MaeIII GTNAC 1 cut(s) 21
MalI GATC 6 cut(s) 469, 521, 732, 759, 809, 1049
MboI GATC 6 cut(s) 467, 519, 730, 757, 807, 1047
MboII GAAGA 7 cut(s) 153, 404, 436, 633, 640, 679, 902
MfeI CAATTG 1 cut(s) 599
MflI RGATCY 1 cut(s) 467
MhlI GDGCHC 1 cut(s) 625
MluCI AATT 4 cut(s) 265, 599, 737, 868
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 1 cut(s) 411
Mph1103I ATGCAT 1 cut(s) 818
MseI TTAA 4 cut(s) 474, 495, 933, 1096
MslI CAYNNNNRTG 3 cut(s) 797, 815, 885
MspA1I CMGCKG 1 cut(s) 847
MunI CAATTG 1 cut(s) 599
Mva1269I GAATGC 1 cut(s) 287
MvnI CGCG 1 cut(s) 847
MwoI GCNNNNNNNGC 1 cut(s) 970
NdeI CATATG 1 cut(s) 812
NdeII GATC 6 cut(s) 467, 519, 730, 757, 807, 1047
NlaIII CATG 8 cut(s) 60, 214, 436, 515, 773, 796, 820, 992
NlaIV GGNNCC 3 cut(s) 385, 469, 782
NsbI TGCGCA 1 cut(s) 939
NsiI ATGCAT 1 cut(s) 818
NspI RCATGY 3 cut(s) 436, 515, 773
PaeI GCATGC 1 cut(s) 436
PceI AGGCCT 1 cut(s) 492
PciI ACATGT 1 cut(s) 769
PctI GAATGC 1 cut(s) 287
PfeI GAWTC 3 cut(s) 305, 526, 581
PkrI GCNGC 4 cut(s) 286, 536, 564, 606
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PscI ACATGT 1 cut(s) 769
PshBI ATTAAT 2 cut(s) 474, 933
Psp124BI GAGCTC 1 cut(s) 625
PspN4I GGNNCC 3 cut(s) 385, 469, 782
PsrI GAACNNNNNNTAC 2 cut(s) 675, 707
PstI CTGCAG 1 cut(s) 286
PstNI CAGNNNCTG 1 cut(s) 1015
PsuI RGATCY 1 cut(s) 467
RsaI GTAC 5 cut(s) 709, 768, 866, 953, 1075
RsaNI GTAC 5 cut(s) 708, 767, 865, 952, 1074
RseI CAYNNNNRTG 3 cut(s) 797, 815, 885
SacI GAGCTC 1 cut(s) 625
SacII CCGCGG 1 cut(s) 848
SaqAI TTAA 4 cut(s) 474, 495, 933, 1096
SatI GCNGC 4 cut(s) 285, 535, 563, 605
Sau3AI GATC 6 cut(s) 467, 519, 730, 757, 807, 1047
SchI GAGTC 1 cut(s) 272
SduI GDGCHC 1 cut(s) 625
SfaNI GCATC 2 cut(s) 827, 994
SfcI CTRYAG 2 cut(s) 282, 454
Sfr303I CCGCGG 1 cut(s) 848
SgrBI CCGCGG 1 cut(s) 848
SmiMI CAYNNNNRTG 3 cut(s) 797, 815, 885
SmlI CTYRAG 1 cut(s) 231
SmoI CTYRAG 1 cut(s) 231
SphI GCATGC 1 cut(s) 436
Sse9I AATT 4 cut(s) 265, 599, 737, 868
SseBI AGGCCT 1 cut(s) 492
SsiI CCGC 4 cut(s) 321, 568, 845, 847
SspI AATATT 1 cut(s) 825
SspMI CTAG 1 cut(s) 197
SstI GAGCTC 1 cut(s) 625
StuI AGGCCT 1 cut(s) 492
StyI CCWWGG 2 cut(s) 911, 1033
TaaI ACNGT 2 cut(s) 356, 458
TaqI TCGA 1 cut(s) 254
TasI AATT 4 cut(s) 265, 599, 737, 868
TatI WGTACW 2 cut(s) 864, 951
TfiI GAWTC 3 cut(s) 305, 526, 581
Tru1I TTAA 4 cut(s) 474, 495, 933, 1096
Tru9I TTAA 4 cut(s) 474, 495, 933, 1096
TscAI CASTG 2 cut(s) 67, 106
TseI GCWGC 4 cut(s) 284, 534, 562, 604
TspDTI ATGAA 8 cut(s) 17, 161, 179, 622, 667, 809, 903, 977
TspGWI ACGGA 1 cut(s) 793
TspRI CASTG 2 cut(s) 67, 106
VspI ATTAAT 2 cut(s) 474, 933
XapI RAATTY 1 cut(s) 737
XceI RCATGY 3 cut(s) 436, 515, 773
XspI CTAG 1 cut(s) 197
Zsp2I ATGCAT 1 cut(s) 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.