Rw1G011220

GDSL esterase lipase At1g71250-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0014790
Physical Location & Seq
Reverse (-)
25328363 .. 25329721
1359 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G011220.1

Sequence Viewer

Length: 1200 bp
ATGCAAGCCACACAACAGAACCAAAGTAAAATGACAAAGATGAAAGAAACCCTCTATTCCAAAACAAAGGGTTTGCTTCTCGTTCATCATCTAGCTCTATTGGTCATGGGGTTTATGGTTTTAGCTCAAGGGAAACTGAAACAGGCTTCGAGTGTCCAAAATTGTAGAAATGACTCTGCAGCATTCTACATATTGGGGGATTCCTCTGTTGATTGCGGAGACAACACGCTCTTTTACCCTCTCATTCACAGTTATCTGTCCTTGTATTCGTGTAATGGTTCTGACGGCTCAACTCTTATTCCCCATCTTCTTGCTGAGAAGATGGGCATGCCAAACATTTTACCATTCTACAGTCAAAATGGATCCATTAATGGAATACAAAGAGGCCTTAACTTCGGTTCAGCACATGCAACGATCTTGAATCACGGCAGCCAGAGTTATCAGTCTGTGAACCAGCAGCTCCGCCAAGTTTTTGATTCTATGCAACTCTTACAATTGCAGCTTAGCGAGGACAGAGCTCTCAGTTTCATCAAATCTTCCATCTTCTACCTCTCCTTTGGCAAAGATGACTTCATAGAGCTCTTCCTACGCAATGCCTCAGGTACTGCAAAACAGAATAATACCGATCAAGGATTTTCACACATTTTAGCAGATCAGATGGTACATGTTATACGGAACCTTTATGACATGAATGTGAGGAGGATCATATGCATGGGAATATTGCCGTTGGGATGCACCCCGCGGGTGTTATTGAACCAGTACAATTCCACAGCCCATCCAAATGAAGATGATGATGATGATGATGTTCCAAGGGGATGTGTAGAGGAGATTAATGCACAGGTCTTAGAGTACAACAGAGAGCTGAATGAGCAAATCTCTGAGCTTCATGCAGAGTTGCCTGATGCACAGCTTCTGTTTTGTGATGTTTACCAAGGACTTGCAACGATCATAAACAACCCAGAACAATATGGATTTGAGGATGTAAAGAATGCTTGCTGCGGGCTTGGTATGTATGGAGCAGAAATTGGATGCCTCTCTAAAGATATGGCATGTGACAAAGAAGCAAGTCATGTGTGGTGGGATTTGTACAATCCTACAATGGCAGTGAACTCATTGTTGGCTGATTCAGCCTGGTCGAGCTATCCGCTTCGGATTTGTCGTCCTATGAGCATCCAGGAATTTTTTACTGCTTCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

399

Amino Acids

44.59

Weight (kDa)

5.09

Isoelectric Point (pI)

47.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 63 - 375 1e-20 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014790)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06121 FvH4_7g06190
malus_domestica MD00G1178200.v1.1
prunus_persica Prupe.6G152900_v2.0.a1
pyrus_communis pycom11g15560
rosa_chinensis RchiOBHm_Chr1g0333821
rosa_laevigata RLG00000029528
rosa_multiflora Rmu_sc0000026.1_g000017 Rmu_sc0007177.1_g000005
rosa_roxburghii Rroxscaffold_4G00317200
rosa_rugosa Rorug01G0112100
rosa_samantha Rh1AG134500 Rh1AG134600 Rh1BG102800 Rh1CG127900 Rh1DG140000
rosa_wichuraiana Rw1G011220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 742
AciI CCGC 6 cut(s) 216, 463, 740, 742, 999, 1145
AclWI GGATC 3 cut(s) 357, 370, 710
AcsI RAATTY 1 cut(s) 1178
AcuI CTGAAG 1 cut(s) 1176
AfaI GTAC 5 cut(s) 604, 663, 761, 851, 1088
AflIII ACRYGT 1 cut(s) 664
AgsI TTSAA 2 cut(s) 421, 754
AjnI CCWGG 2 cut(s) 1130, 1173
AjuI GAANNNNNNNTTGG 2 cut(s) 1100, 1132
Alw21I GWGCWC 2 cut(s) 520, 582
Alw26I GTCTC 1 cut(s) 213
AlwI GGATC 3 cut(s) 357, 370, 710
AlwNI CAGNNNCTG 2 cut(s) 605, 913
AoxI GGCC 1 cut(s) 385
ApeKI GCWGC 5 cut(s) 179, 429, 457, 499, 996
ApoI RAATTY 1 cut(s) 1178
AseI ATTAAT 2 cut(s) 369, 831
AxyI CCTNAGG 1 cut(s) 598
BamHI GGATCC 1 cut(s) 362
BanII GRGCYC 2 cut(s) 520, 582
Bbv12I GWGCWC 2 cut(s) 520, 582
BbvI GCAGC 5 cut(s) 191, 441, 469, 511, 983
BccI CCATC 5 cut(s) 312, 316, 548, 652, 783
BceAI ACGGC 3 cut(s) 301, 442, 709
BciT130I CCWGG 2 cut(s) 1132, 1175
BcoDI GTCTC 1 cut(s) 213
BfaI CTAG 1 cut(s) 92
BfmI CTRYAG 2 cut(s) 177, 349
BisI GCNGC 5 cut(s) 180, 430, 458, 500, 997
BlpI GCTNAGC 1 cut(s) 503
BlsI GCNGC 5 cut(s) 181, 431, 459, 501, 998
Bme1390I CCNGG 2 cut(s) 1132, 1175
BmiI GGNNCC 2 cut(s) 364, 677
BmrFI CCNGG 2 cut(s) 1132, 1175
BmsI GCATC 4 cut(s) 722, 892, 1019, 1179
BplI GAGNNNNNCTC 2 cut(s) 860, 892
Bpu1102I GCTNAGC 1 cut(s) 503
BpuEI CTTGAG 1 cut(s) 111
BsaJI CCNNGG 3 cut(s) 740, 809, 931
Bse1I ACTGG 1 cut(s) 757
Bse21I CCTNAGG 1 cut(s) 598
Bse3DI GCAATG 1 cut(s) 598
BseBI CCWGG 2 cut(s) 1132, 1175
BseDI CCNNGG 3 cut(s) 740, 809, 931
BseGI GGATG 6 cut(s) 737, 775, 821, 985, 1034, 1170
BseMI GCAATG 1 cut(s) 598
BseMII CTCAG 4 cut(s) 306, 535, 612, 870
BseNI ACTGG 1 cut(s) 757
BseRI GAGGAG 2 cut(s) 712, 839
BseXI GCAGC 5 cut(s) 191, 441, 469, 511, 983
Bsh1236I CGCG 1 cut(s) 742
BshFI GGCC 1 cut(s) 387
BsiHKAI GWGCWC 2 cut(s) 520, 582
BsmAI GTCTC 1 cut(s) 213
BsmI GAATGC 2 cut(s) 182, 994
BsnI GGCC 1 cut(s) 387
Bsp1286I GDGCHC 2 cut(s) 520, 582
Bsp1407I TGTACA 1 cut(s) 1086
Bsp143I GATC 6 cut(s) 362, 414, 625, 652, 702, 945
Bsp1720I GCTNAGC 1 cut(s) 503
BspACI CCGC 6 cut(s) 216, 463, 740, 742, 999, 1145
BspANI GGCC 1 cut(s) 387
BspCNI CTCAG 4 cut(s) 307, 534, 611, 871
BspFNI CGCG 1 cut(s) 742
BspLI GGNNCC 2 cut(s) 364, 677
BspMAI CTGCAG 1 cut(s) 181
BspPI GGATC 3 cut(s) 357, 370, 710
BspQI GCTCTTC 1 cut(s) 587
BsrDI GCAATG 1 cut(s) 598
BsrGI TGTACA 1 cut(s) 1086
BsrI ACTGG 1 cut(s) 757
BssECI CCNNGG 3 cut(s) 740, 809, 931
BssMI GATC 6 cut(s) 362, 414, 625, 652, 702, 945
BssT1I CCWWGG 2 cut(s) 809, 931
Bst2UI CCWGG 2 cut(s) 1132, 1175
Bst4CI ACNGT 2 cut(s) 251, 353
Bst6I CTCTTC 1 cut(s) 587
BstAUI TGTACA 1 cut(s) 1086
BstC8I GCNNGC 4 cut(s) 6, 329, 994, 1001
BstDEI CTNAG 6 cut(s) 315, 503, 521, 598, 844, 879
BstDSI CCRYGG 1 cut(s) 740
BstF5I GGATG 6 cut(s) 737, 775, 821, 985, 1034, 1170
BstFNI CGCG 1 cut(s) 742
BstKTI GATC 6 cut(s) 365, 417, 628, 655, 705, 948
BstMAI GTCTC 1 cut(s) 213
BstMBI GATC 6 cut(s) 362, 414, 625, 652, 702, 945
BstMWI GCNNNNNNNGC 2 cut(s) 868, 1127
BstNI CCWGG 2 cut(s) 1132, 1175
BstNSI RCATGY 4 cut(s) 331, 410, 668, 1053
BstSCI CCNGG 2 cut(s) 1130, 1173
BstSFI CTRYAG 2 cut(s) 177, 349
BstUI CGCG 1 cut(s) 742
BstV1I GCAGC 5 cut(s) 191, 441, 469, 511, 983
BstX2I RGATCY 1 cut(s) 362
BstYI RGATCY 1 cut(s) 362
Bsu36I CCTNAGG 1 cut(s) 598
BsuRI GGCC 1 cut(s) 387
BtgI CCRYGG 1 cut(s) 740
BtsCI GGATG 6 cut(s) 737, 775, 821, 985, 1034, 1170
BtsI GCAGTG 1 cut(s) 1110
BtsIMutI CAGTG 1 cut(s) 1110
Cac8I GCNNGC 4 cut(s) 6, 329, 994, 1001
CaiI CAGNNNCTG 2 cut(s) 605, 913
Cfr42I CCGCGG 1 cut(s) 743
Csp6I GTAC 5 cut(s) 603, 662, 760, 850, 1087
CviAII CATG 9 cut(s) 106, 328, 407, 665, 688, 712, 887, 1050, 1070
CviQI GTAC 5 cut(s) 603, 662, 760, 850, 1087
DdeI CTNAG 6 cut(s) 315, 503, 521, 598, 844, 879
DpnI GATC 6 cut(s) 364, 416, 627, 654, 704, 947
DpnII GATC 6 cut(s) 362, 414, 625, 652, 702, 945
Eam1104I CTCTTC 1 cut(s) 587
EarI CTCTTC 1 cut(s) 587
EciI GGCGGA 1 cut(s) 452
Ecl136II GAGCTC 2 cut(s) 518, 580
Eco130I CCWWGG 2 cut(s) 809, 931
Eco147I AGGCCT 1 cut(s) 387
Eco24I GRGCYC 2 cut(s) 520, 582
Eco53kI GAGCTC 2 cut(s) 518, 580
Eco57I CTGAAG 1 cut(s) 1176
Eco81I CCTNAGG 1 cut(s) 598
EcoICRI GAGCTC 2 cut(s) 518, 580
EcoRII CCWGG 2 cut(s) 1130, 1173
EcoT14I CCWWGG 2 cut(s) 809, 931
EcoT22I ATGCAT 1 cut(s) 713
EcoT38I GRGCYC 2 cut(s) 520, 582
ErhI CCWWGG 2 cut(s) 809, 931
FaeI CATG 9 cut(s) 109, 331, 410, 668, 691, 715, 890, 1053, 1073
FatI CATG 9 cut(s) 105, 327, 406, 664, 687, 711, 886, 1049, 1069
FauI CCCGC 3 cut(s) 735, 747, 992
FauNDI CATATG 1 cut(s) 707
Fnu4HI GCNGC 5 cut(s) 180, 430, 458, 500, 997
FokI GGATG 6 cut(s) 744, 762, 828, 992, 1041, 1157
FriOI GRGCYC 2 cut(s) 520, 582
Fsp4HI GCNGC 5 cut(s) 180, 430, 458, 500, 997
FspBI CTAG 1 cut(s) 92
GluI GCNGC 5 cut(s) 180, 430, 458, 500, 997
HaeIII GGCC 1 cut(s) 387
Hin1II CATG 9 cut(s) 109, 331, 410, 668, 691, 715, 890, 1053, 1073
HinfI GANTC 5 cut(s) 173, 200, 421, 476, 1124
Hpy166II GTNNAC 3 cut(s) 451, 928, 1108
Hpy188I TCNGA 4 cut(s) 283, 657, 880, 1152
Hpy188III TCNNGA 1 cut(s) 418
Hpy8I GTNNAC 3 cut(s) 451, 928, 1108
HpyCH4III ACNGT 2 cut(s) 251, 353
HpyF10VI GCNNNNNNNGC 2 cut(s) 868, 1127
HpyF3I CTNAG 6 cut(s) 315, 503, 521, 598, 844, 879
Hsp92II CATG 9 cut(s) 109, 331, 410, 668, 691, 715, 890, 1053, 1073
KspI CCGCGG 1 cut(s) 743
Kzo9I GATC 6 cut(s) 362, 414, 625, 652, 702, 945
LguI GCTCTTC 1 cut(s) 587
LmnI GCTCC 2 cut(s) 465, 1016
Lsp1109I GCAGC 5 cut(s) 191, 441, 469, 511, 983
LweI GCATC 4 cut(s) 722, 892, 1019, 1179
MaeI CTAG 1 cut(s) 92
MaeIII GTNAC 1 cut(s) 1052
MalI GATC 6 cut(s) 364, 416, 627, 654, 704, 947
MboI GATC 6 cut(s) 362, 414, 625, 652, 702, 945
MboII GAAGA 6 cut(s) 299, 331, 528, 535, 574, 797
MfeI CAATTG 1 cut(s) 494
MflI RGATCY 1 cut(s) 362
MhlI GDGCHC 2 cut(s) 520, 582
MluCI AATT 5 cut(s) 160, 494, 763, 1023, 1178
MlyI GAGTC 1 cut(s) 167
Mph1103I ATGCAT 1 cut(s) 713
MseI TTAA 4 cut(s) 369, 390, 831, 1198
MslI CAYNNNNRTG 3 cut(s) 692, 710, 780
MspA1I CMGCKG 1 cut(s) 742
MspR9I CCNGG 2 cut(s) 1132, 1175
MunI CAATTG 1 cut(s) 494
Mva1269I GAATGC 2 cut(s) 182, 994
MvaI CCWGG 2 cut(s) 1132, 1175
MvnI CGCG 1 cut(s) 742
MwoI GCNNNNNNNGC 2 cut(s) 868, 1127
NdeI CATATG 1 cut(s) 707
NdeII GATC 6 cut(s) 362, 414, 625, 652, 702, 945
NlaIII CATG 9 cut(s) 109, 331, 410, 668, 691, 715, 890, 1053, 1073
NlaIV GGNNCC 2 cut(s) 364, 677
NmuCI GTSAC 1 cut(s) 1052
NsiI ATGCAT 1 cut(s) 713
NspI RCATGY 4 cut(s) 331, 410, 668, 1053
PaeI GCATGC 1 cut(s) 331
PceI AGGCCT 1 cut(s) 387
PciI ACATGT 1 cut(s) 664
PciSI GCTCTTC 1 cut(s) 587
PcsI WCGNNNNNNNCGW 1 cut(s) 1156
PctI GAATGC 2 cut(s) 182, 994
PfeI GAWTC 4 cut(s) 200, 421, 476, 1124
PfoI TCCNGGA 1 cut(s) 1173
PkrI GCNGC 5 cut(s) 181, 431, 459, 501, 998
PleI GAGTC 1 cut(s) 167
PpsI GAGTC 1 cut(s) 167
PscI ACATGT 1 cut(s) 664
PshBI ATTAAT 2 cut(s) 369, 831
Psp124BI GAGCTC 2 cut(s) 520, 582
Psp6I CCWGG 2 cut(s) 1130, 1173
PspGI CCWGG 2 cut(s) 1130, 1173
PspN4I GGNNCC 2 cut(s) 364, 677
PstI CTGCAG 1 cut(s) 181
PstNI CAGNNNCTG 2 cut(s) 605, 913
PsuI RGATCY 1 cut(s) 362
RsaI GTAC 5 cut(s) 604, 663, 761, 851, 1088
RsaNI GTAC 5 cut(s) 603, 662, 760, 850, 1087
RseI CAYNNNNRTG 3 cut(s) 692, 710, 780
SacI GAGCTC 2 cut(s) 520, 582
SacII CCGCGG 1 cut(s) 743
SapI GCTCTTC 1 cut(s) 587
SaqAI TTAA 4 cut(s) 369, 390, 831, 1198
SatI GCNGC 5 cut(s) 180, 430, 458, 500, 997
Sau3AI GATC 6 cut(s) 362, 414, 625, 652, 702, 945
SchI GAGTC 1 cut(s) 167
ScrFI CCNGG 2 cut(s) 1132, 1175
SduI GDGCHC 2 cut(s) 520, 582
SfaNI GCATC 4 cut(s) 722, 892, 1019, 1179
SfcI CTRYAG 2 cut(s) 177, 349
Sfr303I CCGCGG 1 cut(s) 743
SgrBI CCGCGG 1 cut(s) 743
SmiMI CAYNNNNRTG 3 cut(s) 692, 710, 780
SmlI CTYRAG 1 cut(s) 126
SmoI CTYRAG 1 cut(s) 126
SphI GCATGC 1 cut(s) 331
Sse9I AATT 5 cut(s) 160, 494, 763, 1023, 1178
SseBI AGGCCT 1 cut(s) 387
SsiI CCGC 6 cut(s) 216, 463, 740, 742, 999, 1145
SspI AATATT 1 cut(s) 720
SspMI CTAG 1 cut(s) 92
SstI GAGCTC 2 cut(s) 520, 582
StuI AGGCCT 1 cut(s) 387
StyD4I CCNGG 2 cut(s) 1130, 1173
StyI CCWWGG 2 cut(s) 809, 931
TaaI ACNGT 2 cut(s) 251, 353
TaqI TCGA 2 cut(s) 149, 1136
TasI AATT 5 cut(s) 160, 494, 763, 1023, 1178
TatI WGTACW 3 cut(s) 759, 849, 1086
TfiI GAWTC 4 cut(s) 200, 421, 476, 1124
Tru1I TTAA 4 cut(s) 369, 390, 831, 1198
Tru9I TTAA 4 cut(s) 369, 390, 831, 1198
TscAI CASTG 1 cut(s) 1110
TseFI GTSAC 1 cut(s) 1052
TseI GCWGC 5 cut(s) 179, 429, 457, 499, 996
Tsp45I GTSAC 1 cut(s) 1052
TspDTI ATGAA 7 cut(s) 56, 74, 517, 562, 704, 798, 875
TspGWI ACGGA 1 cut(s) 688
TspRI CASTG 1 cut(s) 1110
VspI ATTAAT 2 cut(s) 369, 831
XapI RAATTY 1 cut(s) 1178
XceI RCATGY 4 cut(s) 331, 410, 668, 1053
XspI CTAG 1 cut(s) 92
Zsp2I ATGCAT 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.