MD00G1178200.v1.1

GDSL esterase lipase At1g71250-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0014790
Physical Location & Seq
Forward (+)
41603638 .. 41605564
1927 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1178200.v1.1.491

Sequence Viewer

Length: 1278 bp
ATGAGCGAGACTTTGAGCACAAGGAACAAAGCAAAGATGATGGGGTTGGATAGCGCGAAGCTGCCAAAAGGGTCAATTCTGGTTCATCTTCTTGCCCTCTCAGTGATGGGTTTCGTCGTTATAGCTCACGGGAAATTGCCTGAGGTTTCAACAGGGCAGAATGACTCCCCAAATGCGCCCCCTGCATTCTACATTCTTGGGGATTCCTCTGTCGACTGCGGAGACAACACGCTGTTTTACCCACTTGTTCACAGCTCTCTCTCCTTGTATTCCTGCAACGGTTCTTCTGATGCGAGTCTCATTCCTCATCTTCTCGCTGGGAAGATGGGGATGCCGAGTATCCTACCATTCTACAGTCAACAAAACGGATCCATTGACGGAATACTAAGAGGCCTCAACTTTGGTTCGGCACAAGCAACTATCATGAACCCTAGCGGCCAGAGCTACCAGTCCATAAACCAACAACTCCGCCAAGTTTTCGATTCAATGCAGCTCTTACAACTGCAGCTCAGCCAGGATGTAGCCCTCCATTTCATCAGATCATCCATCTTCTACCTCTCCTTTGGCAAAGACGACTACATCAACCTCTACCTACGCAACGCCTCCTCCTCCTCCGGCGCTGAGAAACCCAAGAACAGTGACCAGGAATTTTCCCACATTTTAGCAAATCAGATGATACATGTCATAAGGAACCTGTACGACATGAATGTGAGGAGGATCATATGCATGGGGATATTGCCCTTGGGATGCACGCCGCGTATGTTGTTGGACGGCCACAATTCCAGTTCCACCAATTCCAATTCTGAACCTGGTGACGAAGGAGACGATGATGTCGGGAGTGGATGCGTGGAGGAGATCAACGAGCAGGTCTCGGAGTACAACAAGGTGCTGCACCAGCAGATAGGCAAGCTCCACGCAGAGTTGCCGGATGCACAGATAGTGTTCTGTGATGTGTACCAAGGAATTACAAAGATCATAAACAACCCGAAACGCTATGGATTTGAGGAAGTGAAGAGTGCTTGCTGTGGGTTGGGGATGTACGGAGCAGCAATCGGATGCCTTTCGAAAGAAATGGCGTGCCAACAAGATTCAAGCCACGTGTGGTGGGATTTGTACAACCCAACCAAGGGAGTCAACTCCTTGCTTGCTGACTCCGCCTGGAACGGCTTTCCCCTCTCTCGCATTTGCCGCCCTACCACCATCCAGGAATTACTTTCCACCTCCACTACTACCGCCGCCGCCGCCGCCGCTGCTCCGGTCTCGCATCCTCCCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

426

Amino Acids

46.07

Weight (kDa)

5.61

Isoelectric Point (pI)

54.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 64 - 384 1.3e-18 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014790)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06121 FvH4_7g06190
malus_domestica MD00G1178200.v1.1
prunus_persica Prupe.6G152900_v2.0.a1
pyrus_communis pycom11g15560
rosa_chinensis RchiOBHm_Chr1g0333821
rosa_laevigata RLG00000029528
rosa_multiflora Rmu_sc0000026.1_g000017 Rmu_sc0007177.1_g000005
rosa_roxburghii Rroxscaffold_4G00317200
rosa_rugosa Rorug01G0112100
rosa_samantha Rh1AG134500 Rh1AG134600 Rh1BG102800 Rh1CG127900 Rh1DG140000
rosa_wichuraiana Rw1G011220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 830
Acc36I ACCTGC 1 cut(s) 856
AccI GTMKAC 1 cut(s) 213
AccII CGCG 2 cut(s) 56, 757
AclWI GGATC 3 cut(s) 363, 376, 725
AcoI YGGCCR 2 cut(s) 436, 772
AcsI RAATTY 1 cut(s) 647
AcvI CACGTG 1 cut(s) 1099
AfaI GTAC 5 cut(s) 698, 878, 956, 1040, 1115
AfiI CCNNNNNNNGG 2 cut(s) 1126, 1127
AflIII ACRYGT 2 cut(s) 679, 1098
AgsI TTSAA 3 cut(s) 150, 486, 1092
AjnI CCWGG 5 cut(s) 513, 642, 808, 1157, 1203
AluBI AGCT 7 cut(s) 61, 125, 255, 444, 493, 508, 910
AluI AGCT 7 cut(s) 61, 125, 255, 444, 493, 508, 910
Alw21I GWGCWC 1 cut(s) 20
Alw26I GTCTC 6 cut(s) 2, 216, 302, 816, 874, 1264
AlwI GGATC 3 cut(s) 363, 376, 725
AoxI GGCC 3 cut(s) 391, 436, 772
ApeKI GCWGC 6 cut(s) 61, 490, 505, 889, 1046, 1250
ApoI RAATTY 1 cut(s) 647
AspLEI GCGC 3 cut(s) 56, 178, 620
AsuHPI GGTGA 1 cut(s) 824
AsuII TTCGAA 1 cut(s) 1064
AxyI CCTNAGG 1 cut(s) 141
BamHI GGATCC 1 cut(s) 368
BbrPI CACGTG 1 cut(s) 1099
Bbv12I GWGCWC 1 cut(s) 20
BbvI GCAGC 6 cut(s) 48, 502, 517, 876, 1058, 1237
BccI CCATC 5 cut(s) 34, 100, 319, 554, 1208
BceAI ACGGC 2 cut(s) 787, 1180
BciT130I CCWGG 5 cut(s) 515, 644, 810, 1159, 1205
BciVI GTATCC 1 cut(s) 350
BcoDI GTCTC 6 cut(s) 2, 216, 302, 816, 874, 1264
BfaI CTAG 1 cut(s) 432
BfmI CTRYAG 2 cut(s) 352, 503
BfoI RGCGCY 1 cut(s) 621
BfuAI ACCTGC 1 cut(s) 856
BfuI GTATCC 1 cut(s) 350
BlpI GCTNAGC 1 cut(s) 509
Bme1390I CCNGG 5 cut(s) 515, 644, 810, 1159, 1205
BmiI GGNNCC 2 cut(s) 370, 692
BmrFI CCNGG 5 cut(s) 515, 644, 810, 1159, 1205
BmsI GCATC 7 cut(s) 280, 321, 737, 833, 919, 1046, 1273
BplI GAGNNNNNCTC 2 cut(s) 854, 886
Bpu1102I GCTNAGC 1 cut(s) 509
Bpu14I TTCGAA 1 cut(s) 1064
BsaAI YACGTR 1 cut(s) 1099
BsaI GGTCTC 2 cut(s) 874, 1264
BsaJI CCNNGG 3 cut(s) 741, 958, 1125
BsaWI WCCGGW 1 cut(s) 1255
BsaXI ACNNNNNCTCC 2 cut(s) 590, 620
Bsc4I CCNNNNNNNGG 2 cut(s) 1126, 1127
Bse1I ACTGG 2 cut(s) 448, 783
Bse21I CCTNAGG 1 cut(s) 141
BseBI CCWGG 5 cut(s) 515, 644, 810, 1159, 1205
BseDI CCNNGG 3 cut(s) 741, 958, 1125
BseLI CCNNNNNNNGG 2 cut(s) 1126, 1127
BseMII CTCAG 4 cut(s) 114, 132, 523, 612
BseNI ACTGG 2 cut(s) 448, 783
BseRI GAGGAG 5 cut(s) 595, 598, 601, 727, 866
BseXI GCAGC 6 cut(s) 48, 502, 517, 876, 1058, 1237
BseYI CCCAGC 1 cut(s) 317
BsgI GTGCAG 1 cut(s) 875
Bsh1236I CGCG 2 cut(s) 56, 757
BshFI GGCC 3 cut(s) 393, 438, 774
BsiHKAI GWGCWC 1 cut(s) 20
BsiSI CCGG 3 cut(s) 615, 926, 1256
BslI CCNNNNNNNGG 2 cut(s) 1126, 1127
BsmAI GTCTC 6 cut(s) 2, 216, 302, 816, 874, 1264
BsmBI CGTCTC 1 cut(s) 816
BsmI GAATGC 1 cut(s) 185
BsnI GGCC 3 cut(s) 393, 438, 774
Bso31I GGTCTC 2 cut(s) 874, 1264
Bsp119I TTCGAA 1 cut(s) 1064
Bsp1286I GDGCHC 1 cut(s) 20
Bsp1407I TGTACA 1 cut(s) 1113
Bsp143I GATC 5 cut(s) 368, 539, 717, 855, 972
Bsp1720I GCTNAGC 1 cut(s) 509
BspANI GGCC 3 cut(s) 393, 438, 774
BspCNI CTCAG 4 cut(s) 113, 133, 522, 613
BspFNI CGCG 2 cut(s) 56, 757
BspHI TCATGA 1 cut(s) 423
BspLI GGNNCC 2 cut(s) 370, 692
BspMAI CTGCAG 1 cut(s) 507
BspMI ACCTGC 1 cut(s) 856
BspPI GGATC 3 cut(s) 363, 376, 725
BspT104I TTCGAA 1 cut(s) 1064
BspTNI GGTCTC 2 cut(s) 874, 1264
BsrGI TGTACA 1 cut(s) 1113
BsrI ACTGG 2 cut(s) 448, 783
BssECI CCNNGG 3 cut(s) 741, 958, 1125
BssMI GATC 5 cut(s) 368, 539, 717, 855, 972
BssT1I CCWWGG 3 cut(s) 741, 958, 1125
Bst2UI CCWGG 5 cut(s) 515, 644, 810, 1159, 1205
Bst4CI ACNGT 3 cut(s) 281, 356, 638
Bst6I CTCTTC 1 cut(s) 1007
BstAUI TGTACA 1 cut(s) 1113
BstBAI YACGTR 1 cut(s) 1099
BstBI TTCGAA 1 cut(s) 1064
BstC8I GCNNGC 5 cut(s) 752, 908, 1021, 1078, 1146
BstDEI CTNAG 5 cut(s) 100, 141, 386, 509, 621
BstFNI CGCG 2 cut(s) 56, 757
BstH2I RGCGCY 1 cut(s) 621
BstHHI GCGC 3 cut(s) 56, 178, 620
BstKTI GATC 5 cut(s) 371, 542, 720, 858, 975
BstMAI GTCTC 6 cut(s) 2, 216, 302, 816, 874, 1264
BstMBI GATC 5 cut(s) 368, 539, 717, 855, 972
BstMWI GCNNNNNNNGC 9 cut(s) 182, 441, 895, 1154, 1188, 1241, 1244, 1247, 1250
BstNI CCWGG 5 cut(s) 515, 644, 810, 1159, 1205
BstNSI RCATGY 1 cut(s) 683
BstSCI CCNGG 5 cut(s) 513, 642, 808, 1157, 1203
BstSFI CTRYAG 2 cut(s) 352, 503
BstUI CGCG 2 cut(s) 56, 757
BstV1I GCAGC 6 cut(s) 48, 502, 517, 876, 1058, 1237
BstX2I RGATCY 1 cut(s) 368
BstYI RGATCY 1 cut(s) 368
Bsu36I CCTNAGG 1 cut(s) 141
BsuI GTATCC 1 cut(s) 350
BsuRI GGCC 3 cut(s) 393, 438, 774
BtsIMutI CAGTG 2 cut(s) 108, 643
BveI ACCTGC 1 cut(s) 856
Cac8I GCNNGC 5 cut(s) 752, 908, 1021, 1078, 1146
CciI TCATGA 1 cut(s) 423
CfoI GCGC 3 cut(s) 56, 178, 620
CsiI ACCWGGT 1 cut(s) 808
Csp6I GTAC 5 cut(s) 697, 877, 955, 1039, 1114
CviAII CATG 4 cut(s) 424, 680, 703, 727
CviQI GTAC 5 cut(s) 697, 877, 955, 1039, 1114
DdeI CTNAG 5 cut(s) 100, 141, 386, 509, 621
DpnI GATC 5 cut(s) 370, 541, 719, 857, 974
DpnII GATC 5 cut(s) 368, 539, 717, 855, 972
DrdI GACNNNNNNGTC 1 cut(s) 830
DseDI GACNNNNNNGTC 1 cut(s) 830
EaeI YGGCCR 2 cut(s) 436, 772
Eam1104I CTCTTC 1 cut(s) 1007
EarI CTCTTC 1 cut(s) 1007
EciI GGCGGA 2 cut(s) 458, 1144
Eco130I CCWWGG 3 cut(s) 741, 958, 1125
Eco147I AGGCCT 1 cut(s) 393
Eco31I GGTCTC 2 cut(s) 874, 1264
Eco72I CACGTG 1 cut(s) 1099
Eco81I CCTNAGG 1 cut(s) 141
EcoRII CCWGG 5 cut(s) 513, 642, 808, 1157, 1203
EcoT14I CCWWGG 3 cut(s) 741, 958, 1125
EcoT22I ATGCAT 1 cut(s) 728
ErhI CCWWGG 3 cut(s) 741, 958, 1125
Esp3I CGTCTC 1 cut(s) 816
FaeI CATG 4 cut(s) 427, 683, 706, 730
FatI CATG 4 cut(s) 423, 679, 702, 726
FauNDI CATATG 1 cut(s) 722
FblI GTMKAC 1 cut(s) 213
FspBI CTAG 1 cut(s) 432
GlaI GCGC 3 cut(s) 55, 177, 619
GsaI CCCAGC 1 cut(s) 321
HaeII RGCGCY 1 cut(s) 621
HaeIII GGCC 3 cut(s) 393, 438, 774
HapII CCGG 3 cut(s) 615, 926, 1256
HhaI GCGC 3 cut(s) 56, 178, 620
Hin1II CATG 4 cut(s) 427, 683, 706, 730
Hin6I GCGC 3 cut(s) 54, 176, 618
HinP1I GCGC 3 cut(s) 54, 176, 618
HincII GTYRAC 3 cut(s) 214, 359, 1135
HindII GTYRAC 3 cut(s) 214, 359, 1135
HinfI GANTC 7 cut(s) 164, 203, 295, 482, 1088, 1131, 1151
HpaII CCGG 3 cut(s) 615, 926, 1256
HphI GGTGA 1 cut(s) 824
Hpy166II GTNNAC 5 cut(s) 214, 250, 359, 955, 1135
Hpy188I TCNGA 6 cut(s) 289, 539, 672, 805, 874, 1055
Hpy188III TCNNGA 2 cut(s) 424, 835
Hpy8I GTNNAC 5 cut(s) 214, 250, 359, 955, 1135
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 1 cut(s) 812
HpyCH4III ACNGT 3 cut(s) 281, 356, 638
HpyCH4IV ACGT 1 cut(s) 1098
HpyCH4V TGCA 8 cut(s) 185, 276, 490, 505, 726, 750, 892, 932
HpyF10VI GCNNNNNNNGC 9 cut(s) 182, 441, 895, 1154, 1188, 1241, 1244, 1247, 1250
HpyF3I CTNAG 5 cut(s) 100, 141, 386, 509, 621
HpySE526I ACGT 1 cut(s) 1098
Hsp92II CATG 4 cut(s) 427, 683, 706, 730
HspAI GCGC 3 cut(s) 54, 176, 618
Kzo9I GATC 5 cut(s) 368, 539, 717, 855, 972
LmnI GCTCC 3 cut(s) 915, 1043, 1258
Lsp1109I GCAGC 6 cut(s) 48, 502, 517, 876, 1058, 1237
LweI GCATC 7 cut(s) 280, 321, 737, 833, 919, 1046, 1273
MabI ACCWGGT 1 cut(s) 808
MaeI CTAG 1 cut(s) 432
MaeII ACGT 1 cut(s) 1098
MaeIII GTNAC 2 cut(s) 638, 812
MalI GATC 5 cut(s) 370, 541, 719, 857, 974
MboI GATC 5 cut(s) 368, 539, 717, 855, 972
MboII GAAGA 6 cut(s) 80, 276, 302, 334, 541, 1024
MflI RGATCY 1 cut(s) 368
MhlI GDGCHC 1 cut(s) 20
MluCI AATT 8 cut(s) 75, 134, 647, 778, 793, 799, 963, 1208
MlyI GAGTC 4 cut(s) 158, 304, 1140, 1145
MmeI TCCRAC 2 cut(s) 27, 747
Mph1103I ATGCAT 1 cut(s) 728
MslI CAYNNNNRTG 2 cut(s) 707, 725
MspA1I CMGCKG 1 cut(s) 1250
MspI CCGG 3 cut(s) 615, 926, 1256
MspR9I CCNGG 5 cut(s) 515, 644, 810, 1159, 1205
Mva1269I GAATGC 1 cut(s) 185
MvaI CCWGG 5 cut(s) 515, 644, 810, 1159, 1205
MvnI CGCG 2 cut(s) 56, 757
MwoI GCNNNNNNNGC 9 cut(s) 182, 441, 895, 1154, 1188, 1241, 1244, 1247, 1250
NdeI CATATG 1 cut(s) 722
NdeII GATC 5 cut(s) 368, 539, 717, 855, 972
NlaIII CATG 4 cut(s) 427, 683, 706, 730
NlaIV GGNNCC 2 cut(s) 370, 692
NmeAIII GCCGAG 1 cut(s) 360
NmuCI GTSAC 2 cut(s) 638, 812
NsiI ATGCAT 1 cut(s) 728
NspI RCATGY 1 cut(s) 683
NspV TTCGAA 1 cut(s) 1064
PagI TCATGA 1 cut(s) 423
PceI AGGCCT 1 cut(s) 393
PciI ACATGT 1 cut(s) 679
PcsI WCGNNNNNNNCGW 1 cut(s) 822
PctI GAATGC 1 cut(s) 185
PfeI GAWTC 3 cut(s) 203, 482, 1088
PfoI TCCNGGA 1 cut(s) 1203
PleI GAGTC 4 cut(s) 158, 303, 1139, 1145
PmaCI CACGTG 1 cut(s) 1099
PmlI CACGTG 1 cut(s) 1099
PpsI GAGTC 4 cut(s) 158, 303, 1139, 1145
Ppu21I YACGTR 1 cut(s) 1099
PscI ACATGT 1 cut(s) 679
Psp6I CCWGG 5 cut(s) 513, 642, 808, 1157, 1203
PspCI CACGTG 1 cut(s) 1099
PspFI CCCAGC 1 cut(s) 317
PspGI CCWGG 5 cut(s) 513, 642, 808, 1157, 1203
PspN4I GGNNCC 2 cut(s) 370, 692
PstI CTGCAG 1 cut(s) 507
PsuI RGATCY 1 cut(s) 368
RsaI GTAC 5 cut(s) 698, 878, 956, 1040, 1115
RsaNI GTAC 5 cut(s) 697, 877, 955, 1039, 1114
RseI CAYNNNNRTG 2 cut(s) 707, 725
SalI GTCGAC 1 cut(s) 212
Sau3AI GATC 5 cut(s) 368, 539, 717, 855, 972
SchI GAGTC 4 cut(s) 158, 304, 1140, 1145
ScrFI CCNGG 5 cut(s) 515, 644, 810, 1159, 1205
SduI GDGCHC 1 cut(s) 20
SexAI ACCWGGT 1 cut(s) 808
SfaNI GCATC 7 cut(s) 280, 321, 737, 833, 919, 1046, 1273
SfcI CTRYAG 2 cut(s) 352, 503
SfuI TTCGAA 1 cut(s) 1064
SmiMI CAYNNNNRTG 2 cut(s) 707, 725
Sse9I AATT 8 cut(s) 75, 134, 647, 778, 793, 799, 963, 1208
SseBI AGGCCT 1 cut(s) 393
SspMI CTAG 1 cut(s) 432
StuI AGGCCT 1 cut(s) 393
StyD4I CCNGG 5 cut(s) 513, 642, 808, 1157, 1203
StyI CCWWGG 3 cut(s) 741, 958, 1125
TaaI ACNGT 3 cut(s) 281, 356, 638
TaiI ACGT 1 cut(s) 1101
TaqI TCGA 3 cut(s) 213, 480, 1064
TasI AATT 8 cut(s) 75, 134, 647, 778, 793, 799, 963, 1208
TatI WGTACW 2 cut(s) 876, 1113
TauI GCSGC 8 cut(s) 438, 757, 1191, 1238, 1241, 1244, 1247, 1250
TfiI GAWTC 3 cut(s) 203, 482, 1088
TscAI CASTG 2 cut(s) 108, 643
TseFI GTSAC 2 cut(s) 638, 812
TseI GCWGC 6 cut(s) 61, 490, 505, 889, 1046, 1250
Tsp45I GTSAC 2 cut(s) 638, 812
TspDTI ATGAA 4 cut(s) 74, 440, 523, 719
TspGWI ACGGA 3 cut(s) 381, 393, 1056
TspRI CASTG 2 cut(s) 108, 643
XapI RAATTY 1 cut(s) 647
XceI RCATGY 1 cut(s) 683
XmiI GTMKAC 1 cut(s) 213
XspI CTAG 1 cut(s) 432
Zsp2I ATGCAT 1 cut(s) 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.