Rroxscaffold_4G00317200

GDSL esterase lipase At1g71250-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0014790
Physical Location & Seq
Forward (+)
42345486 .. 42346877
1392 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00317200.1

Sequence Viewer

Length: 1230 bp
ATGCTGAACGCAACATTAGTATCAAGCAGTGAAATGCAAGCCACACAACAGAACCAAAGTAACATGACAAAGATGAAAGAAACCATCTCTTCCAAAACAAAGGGTTTGCTTCTCGTTCATCATCTAGCTCTATTGGTCATGGGGTTTATGGTTTTAGCTCAAGGGAAACTGAAACAGGCTTCGAGTGTCCAAAATTGTAGAAATGACTCTGCAGCATTCTACATATTGGGGGATTCCTCTGTTGATTGCGGAGACAACACGCTCTTTTACCCTCTCATTCACAGTTATCTGTCCTTGTATTCGTGTAATGGTTCCGACGGCTCAACTCTTATTCCCCATCTTCTTGCTGAGAAGATGGGCATGCCAAACATTTTACCATTCTACAGTCAAAATGGATCCATTAATGGAATACAAAGAGGCCTTAACTTCGGTTCAGCACATGCAACGATCTTGAATCACGGCAGTCAGAGTTATCAGTCTGTGAACCAGCAGCTCCGCCAAGTTTTTGATTCTATGCAACTCTTACAATTGCAGCTTAGCGAGGACAGAGCTCTCAATTTCATCAAATCTTCCATCTTCTACCTCTCCTTTGGCAAAGATGACTTCATAGAGCTCTTCCTACGCAATGCCTCGGGTACTGCAAAACAGAATAATACCGATCAAGAATTTTCACACATTTTAGCAGATCAGATGGTACATGTTATACGGAACCTTTATGACATGAATGTGAGGAGGATCATATGCATGGGAATATTGCCGTTGGGATGCACCCCGCGGGTGTTATTGAACCAGTACAATTCCACAGCCCATCCAGATGAAGATGATGATGATGATGTTGCAAGGGGATGTGTAGAGGAGATTAATGCGCAGGTCTTAGAGTACAACAGAGAGCTGAATGAGCAAATCTCTGAGCTTCATGCAGAGTTGCCTGATGCACAGCTTCTGTTTTGTGATGTTTACCAAGGACTTGCAACGATCATAAACAACCCAGAACAATATGGATTTGAGGATGTAAAGAATGCTTGCTGCGGGCTTGGTATGTATGGAGCAGAAATTGGATGCCTCTCTAAAGATATGGCATGTGACAAAGAAGCAAGTCACGTGTGGTGGGATTTATACAATCCTACAAAGGCGGTGAACTCATTGCTGGCTGATTCAGCCTGGTCGAGCTATCCGCTTCGGATTTGTCGTCCTATGAGCATCCAGGAATTTTTTACTGCTTCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

409

Amino Acids

45.63

Weight (kDa)

4.98

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 74 - 385 1.5e-21 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014790)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06121 FvH4_7g06190
malus_domestica MD00G1178200.v1.1
prunus_persica Prupe.6G152900_v2.0.a1
pyrus_communis pycom11g15560
rosa_chinensis RchiOBHm_Chr1g0333821
rosa_laevigata RLG00000029528
rosa_multiflora Rmu_sc0000026.1_g000017 Rmu_sc0007177.1_g000005
rosa_roxburghii Rroxscaffold_4G00317200
rosa_rugosa Rorug01G0112100
rosa_samantha Rh1AG134500 Rh1AG134600 Rh1BG102800 Rh1CG127900 Rh1DG140000
rosa_wichuraiana Rw1G011220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 867
Acc36I ACCTGC 1 cut(s) 859
AccII CGCG 1 cut(s) 775
AciI CCGC 7 cut(s) 249, 496, 773, 775, 1029, 1133, 1175
AclWI GGATC 3 cut(s) 390, 403, 743
AcsI RAATTY 2 cut(s) 665, 1208
AcvI CACGTG 1 cut(s) 1102
AfaI GTAC 4 cut(s) 637, 696, 794, 881
AflIII ACRYGT 2 cut(s) 697, 1101
AgsI TTSAA 2 cut(s) 454, 787
AjnI CCWGG 2 cut(s) 1160, 1203
Alw21I GWGCWC 2 cut(s) 553, 615
Alw26I GTCTC 1 cut(s) 246
AlwI GGATC 3 cut(s) 390, 403, 743
AlwNI CAGNNNCTG 1 cut(s) 943
Ama87I CYCGRG 1 cut(s) 631
AoxI GGCC 1 cut(s) 418
ApeKI GCWGC 4 cut(s) 212, 490, 532, 1026
ApoI RAATTY 2 cut(s) 665, 1208
AseI ATTAAT 2 cut(s) 402, 861
AspLEI GCGC 1 cut(s) 868
AsuHPI GGTGA 1 cut(s) 1147
AvaI CYCGRG 1 cut(s) 631
BaeI ACNNNNGTAYC 1 cut(s) 36
BamHI GGATCC 1 cut(s) 395
BanII GRGCYC 2 cut(s) 553, 615
BbrPI CACGTG 1 cut(s) 1102
Bbv12I GWGCWC 2 cut(s) 553, 615
BbvI GCAGC 4 cut(s) 224, 502, 544, 1013
BccI CCATC 6 cut(s) 92, 345, 349, 581, 685, 816
BceAI ACGGC 3 cut(s) 334, 475, 742
BciT130I CCWGG 2 cut(s) 1162, 1205
BcoDI GTCTC 1 cut(s) 246
BfaI CTAG 1 cut(s) 125
BfmI CTRYAG 2 cut(s) 210, 382
BfuAI ACCTGC 1 cut(s) 859
BisI GCNGC 4 cut(s) 213, 491, 533, 1027
BlpI GCTNAGC 1 cut(s) 536
BlsI GCNGC 4 cut(s) 214, 492, 534, 1028
Bme1390I CCNGG 2 cut(s) 1162, 1205
BmeT110I CYCGRG 1 cut(s) 631
BmiI GGNNCC 3 cut(s) 313, 397, 710
BmrFI CCNGG 2 cut(s) 1162, 1205
BmsI GCATC 4 cut(s) 755, 922, 1049, 1209
BplI GAGNNNNNCTC 2 cut(s) 890, 922
Bpu1102I GCTNAGC 1 cut(s) 536
BpuEI CTTGAG 1 cut(s) 144
BsaAI YACGTR 1 cut(s) 1102
BsaJI CCNNGG 3 cut(s) 630, 773, 961
Bse1I ACTGG 1 cut(s) 790
Bse3DI GCAATG 2 cut(s) 631, 1142
BseBI CCWGG 2 cut(s) 1162, 1205
BseDI CCNNGG 3 cut(s) 630, 773, 961
BseGI GGATG 6 cut(s) 770, 808, 851, 1015, 1064, 1200
BseMI GCAATG 2 cut(s) 631, 1142
BseMII CTCAG 2 cut(s) 339, 900
BseNI ACTGG 1 cut(s) 790
BseRI GAGGAG 2 cut(s) 745, 869
BseXI GCAGC 4 cut(s) 224, 502, 544, 1013
Bsh1236I CGCG 1 cut(s) 775
BshFI GGCC 1 cut(s) 420
BsiHKAI GWGCWC 2 cut(s) 553, 615
BsiHKCI CYCGRG 1 cut(s) 631
BsmAI GTCTC 1 cut(s) 246
BsmI GAATGC 2 cut(s) 215, 1024
BsnI GGCC 1 cut(s) 420
BsoBI CYCGRG 1 cut(s) 631
Bsp1286I GDGCHC 2 cut(s) 553, 615
Bsp143I GATC 6 cut(s) 395, 447, 658, 685, 735, 975
Bsp1720I GCTNAGC 1 cut(s) 536
BspACI CCGC 7 cut(s) 249, 496, 773, 775, 1029, 1133, 1175
BspANI GGCC 1 cut(s) 420
BspCNI CTCAG 2 cut(s) 340, 901
BspFNI CGCG 1 cut(s) 775
BspLI GGNNCC 3 cut(s) 313, 397, 710
BspMAI CTGCAG 1 cut(s) 214
BspMI ACCTGC 1 cut(s) 859
BspPI GGATC 3 cut(s) 390, 403, 743
BspQI GCTCTTC 1 cut(s) 620
BsrDI GCAATG 2 cut(s) 631, 1142
BsrI ACTGG 1 cut(s) 790
BssECI CCNNGG 3 cut(s) 630, 773, 961
BssMI GATC 6 cut(s) 395, 447, 658, 685, 735, 975
BssT1I CCWWGG 1 cut(s) 961
Bst2UI CCWGG 2 cut(s) 1162, 1205
Bst4CI ACNGT 2 cut(s) 284, 386
Bst6I CTCTTC 2 cut(s) 94, 620
BstBAI YACGTR 1 cut(s) 1102
BstC8I GCNNGC 5 cut(s) 39, 362, 1024, 1031, 1149
BstDEI CTNAG 4 cut(s) 348, 536, 874, 909
BstDSI CCRYGG 1 cut(s) 773
BstF5I GGATG 6 cut(s) 770, 808, 851, 1015, 1064, 1200
BstFNI CGCG 1 cut(s) 775
BstHHI GCGC 1 cut(s) 868
BstKTI GATC 6 cut(s) 398, 450, 661, 688, 738, 978
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 6 cut(s) 395, 447, 658, 685, 735, 975
BstMWI GCNNNNNNNGC 2 cut(s) 898, 1157
BstNI CCWGG 2 cut(s) 1162, 1205
BstNSI RCATGY 4 cut(s) 364, 443, 701, 1083
BstSCI CCNGG 2 cut(s) 1160, 1203
BstSFI CTRYAG 2 cut(s) 210, 382
BstUI CGCG 1 cut(s) 775
BstV1I GCAGC 4 cut(s) 224, 502, 544, 1013
BstX2I RGATCY 1 cut(s) 395
BstYI RGATCY 1 cut(s) 395
BsuRI GGCC 1 cut(s) 420
BtgI CCRYGG 1 cut(s) 773
BtsCI GGATG 6 cut(s) 770, 808, 851, 1015, 1064, 1200
BtsI GCAGTG 1 cut(s) 34
BtsIMutI CAGTG 1 cut(s) 34
BveI ACCTGC 1 cut(s) 859
Cac8I GCNNGC 5 cut(s) 39, 362, 1024, 1031, 1149
CaiI CAGNNNCTG 1 cut(s) 943
CfoI GCGC 1 cut(s) 868
Cfr42I CCGCGG 1 cut(s) 776
Csp6I GTAC 4 cut(s) 636, 695, 793, 880
CviAII CATG 9 cut(s) 64, 139, 361, 440, 698, 721, 745, 917, 1080
CviQI GTAC 4 cut(s) 636, 695, 793, 880
DdeI CTNAG 4 cut(s) 348, 536, 874, 909
DpnI GATC 6 cut(s) 397, 449, 660, 687, 737, 977
DpnII GATC 6 cut(s) 395, 447, 658, 685, 735, 975
Eam1104I CTCTTC 2 cut(s) 94, 620
EarI CTCTTC 2 cut(s) 94, 620
EciI GGCGGA 1 cut(s) 485
Ecl136II GAGCTC 2 cut(s) 551, 613
Eco130I CCWWGG 1 cut(s) 961
Eco147I AGGCCT 1 cut(s) 420
Eco24I GRGCYC 2 cut(s) 553, 615
Eco53kI GAGCTC 2 cut(s) 551, 613
Eco72I CACGTG 1 cut(s) 1102
Eco88I CYCGRG 1 cut(s) 631
EcoICRI GAGCTC 2 cut(s) 551, 613
EcoRII CCWGG 2 cut(s) 1160, 1203
EcoT14I CCWWGG 1 cut(s) 961
EcoT22I ATGCAT 1 cut(s) 746
EcoT38I GRGCYC 2 cut(s) 553, 615
ErhI CCWWGG 1 cut(s) 961
FaeI CATG 9 cut(s) 67, 142, 364, 443, 701, 724, 748, 920, 1083
FatI CATG 9 cut(s) 63, 138, 360, 439, 697, 720, 744, 916, 1079
FauI CCCGC 3 cut(s) 768, 780, 1022
FauNDI CATATG 1 cut(s) 740
Fnu4HI GCNGC 4 cut(s) 213, 491, 533, 1027
FokI GGATG 6 cut(s) 777, 795, 858, 1022, 1071, 1187
FriOI GRGCYC 2 cut(s) 553, 615
Fsp4HI GCNGC 4 cut(s) 213, 491, 533, 1027
FspBI CTAG 1 cut(s) 125
FspI TGCGCA 1 cut(s) 867
GlaI GCGC 1 cut(s) 867
GluI GCNGC 4 cut(s) 213, 491, 533, 1027
HaeIII GGCC 1 cut(s) 420
HhaI GCGC 1 cut(s) 868
Hin1II CATG 9 cut(s) 67, 142, 364, 443, 701, 724, 748, 920, 1083
Hin6I GCGC 1 cut(s) 866
HinP1I GCGC 1 cut(s) 866
HinfI GANTC 5 cut(s) 206, 233, 454, 509, 1154
HphI GGTGA 1 cut(s) 1147
Hpy166II GTNNAC 3 cut(s) 484, 958, 1138
Hpy188I TCNGA 5 cut(s) 316, 468, 690, 910, 1182
Hpy188III TCNNGA 3 cut(s) 451, 662, 812
Hpy8I GTNNAC 3 cut(s) 484, 958, 1138
Hpy99I CGWCG 1 cut(s) 320
HpyCH4III ACNGT 2 cut(s) 284, 386
HpyCH4IV ACGT 1 cut(s) 1101
HpyF10VI GCNNNNNNNGC 2 cut(s) 898, 1157
HpyF3I CTNAG 4 cut(s) 348, 536, 874, 909
HpySE526I ACGT 1 cut(s) 1101
Hsp92II CATG 9 cut(s) 67, 142, 364, 443, 701, 724, 748, 920, 1083
HspAI GCGC 1 cut(s) 866
KspI CCGCGG 1 cut(s) 776
Kzo9I GATC 6 cut(s) 395, 447, 658, 685, 735, 975
LguI GCTCTTC 1 cut(s) 620
LmnI GCTCC 2 cut(s) 498, 1046
Lsp1109I GCAGC 4 cut(s) 224, 502, 544, 1013
LweI GCATC 4 cut(s) 755, 922, 1049, 1209
MaeI CTAG 1 cut(s) 125
MaeII ACGT 1 cut(s) 1101
MaeIII GTNAC 3 cut(s) 59, 1082, 1097
MalI GATC 6 cut(s) 397, 449, 660, 687, 737, 977
MboI GATC 6 cut(s) 395, 447, 658, 685, 735, 975
MboII GAAGA 7 cut(s) 81, 332, 364, 561, 568, 607, 830
MfeI CAATTG 1 cut(s) 527
MflI RGATCY 1 cut(s) 395
MhlI GDGCHC 2 cut(s) 553, 615
MluCI AATT 7 cut(s) 193, 527, 556, 665, 796, 1053, 1208
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 339
Mph1103I ATGCAT 1 cut(s) 746
MseI TTAA 4 cut(s) 402, 423, 861, 1228
MslI CAYNNNNRTG 3 cut(s) 725, 743, 813
MspA1I CMGCKG 1 cut(s) 775
MspR9I CCNGG 2 cut(s) 1162, 1205
MunI CAATTG 1 cut(s) 527
Mva1269I GAATGC 2 cut(s) 215, 1024
MvaI CCWGG 2 cut(s) 1162, 1205
MvnI CGCG 1 cut(s) 775
MwoI GCNNNNNNNGC 2 cut(s) 898, 1157
NdeI CATATG 1 cut(s) 740
NdeII GATC 6 cut(s) 395, 447, 658, 685, 735, 975
NlaIII CATG 9 cut(s) 67, 142, 364, 443, 701, 724, 748, 920, 1083
NlaIV GGNNCC 3 cut(s) 313, 397, 710
NmuCI GTSAC 2 cut(s) 1082, 1097
NsbI TGCGCA 1 cut(s) 867
NsiI ATGCAT 1 cut(s) 746
NspI RCATGY 4 cut(s) 364, 443, 701, 1083
PaeI GCATGC 1 cut(s) 364
PceI AGGCCT 1 cut(s) 420
PciI ACATGT 1 cut(s) 697
PciSI GCTCTTC 1 cut(s) 620
PcsI WCGNNNNNNNCGW 1 cut(s) 1186
PctI GAATGC 2 cut(s) 215, 1024
PfeI GAWTC 4 cut(s) 233, 454, 509, 1154
PfoI TCCNGGA 1 cut(s) 1203
PkrI GCNGC 4 cut(s) 214, 492, 534, 1028
PleI GAGTC 1 cut(s) 200
PmaCI CACGTG 1 cut(s) 1102
PmlI CACGTG 1 cut(s) 1102
PpsI GAGTC 1 cut(s) 200
Ppu21I YACGTR 1 cut(s) 1102
PscI ACATGT 1 cut(s) 697
PshBI ATTAAT 2 cut(s) 402, 861
Psp124BI GAGCTC 2 cut(s) 553, 615
Psp6I CCWGG 2 cut(s) 1160, 1203
PspCI CACGTG 1 cut(s) 1102
PspGI CCWGG 2 cut(s) 1160, 1203
PspN4I GGNNCC 3 cut(s) 313, 397, 710
PstI CTGCAG 1 cut(s) 214
PstNI CAGNNNCTG 1 cut(s) 943
PsuI RGATCY 1 cut(s) 395
RsaI GTAC 4 cut(s) 637, 696, 794, 881
RsaNI GTAC 4 cut(s) 636, 695, 793, 880
RseI CAYNNNNRTG 3 cut(s) 725, 743, 813
SacI GAGCTC 2 cut(s) 553, 615
SacII CCGCGG 1 cut(s) 776
SapI GCTCTTC 1 cut(s) 620
SaqAI TTAA 4 cut(s) 402, 423, 861, 1228
SatI GCNGC 4 cut(s) 213, 491, 533, 1027
Sau3AI GATC 6 cut(s) 395, 447, 658, 685, 735, 975
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 2 cut(s) 1162, 1205
SduI GDGCHC 2 cut(s) 553, 615
SfaNI GCATC 4 cut(s) 755, 922, 1049, 1209
SfcI CTRYAG 2 cut(s) 210, 382
Sfr303I CCGCGG 1 cut(s) 776
SgrBI CCGCGG 1 cut(s) 776
SmiMI CAYNNNNRTG 3 cut(s) 725, 743, 813
SmlI CTYRAG 1 cut(s) 159
SmoI CTYRAG 1 cut(s) 159
SphI GCATGC 1 cut(s) 364
Sse9I AATT 7 cut(s) 193, 527, 556, 665, 796, 1053, 1208
SseBI AGGCCT 1 cut(s) 420
SsiI CCGC 7 cut(s) 249, 496, 773, 775, 1029, 1133, 1175
SspI AATATT 1 cut(s) 753
SspMI CTAG 1 cut(s) 125
SstI GAGCTC 2 cut(s) 553, 615
StuI AGGCCT 1 cut(s) 420
StyD4I CCNGG 2 cut(s) 1160, 1203
StyI CCWWGG 1 cut(s) 961
TaaI ACNGT 2 cut(s) 284, 386
TaiI ACGT 1 cut(s) 1104
TaqI TCGA 2 cut(s) 182, 1166
TasI AATT 7 cut(s) 193, 527, 556, 665, 796, 1053, 1208
TatI WGTACW 2 cut(s) 792, 879
TfiI GAWTC 4 cut(s) 233, 454, 509, 1154
Tru1I TTAA 4 cut(s) 402, 423, 861, 1228
Tru9I TTAA 4 cut(s) 402, 423, 861, 1228
TscAI CASTG 1 cut(s) 34
TseFI GTSAC 2 cut(s) 1082, 1097
TseI GCWGC 4 cut(s) 212, 490, 532, 1026
Tsp45I GTSAC 2 cut(s) 1082, 1097
TspDTI ATGAA 8 cut(s) 89, 107, 550, 595, 737, 831, 905, 1212
TspGWI ACGGA 1 cut(s) 721
TspRI CASTG 1 cut(s) 34
VspI ATTAAT 2 cut(s) 402, 861
XapI RAATTY 2 cut(s) 665, 1208
XceI RCATGY 4 cut(s) 364, 443, 701, 1083
XspI CTAG 1 cut(s) 125
Zsp2I ATGCAT 1 cut(s) 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.