RLG00000029528

GDSL esterase lipase At1g71250-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0014790
Physical Location & Seq
Forward (+)
39894406 .. 39897526
3121 bp
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UTR
Exon/CDS
Intron
RLM00000029528

Sequence Viewer

Length: 1188 bp
ATGGCTTTGCTTTTTAAACATGAAAGATTGTTTAATTACTTTGTGGTGAGCAGGTTATCAAAAGACCAAAAGCAGAAGAATATTGCAGCATGCACATCGAAGTCTAAACAATCCTTGTATTTACAAAGAGGATGGGTTGGGTTGGCGGCGGCGGCGGCGGTGGAGGGTGATAGGAGAAGTGGAGTTTGGCAGGGATCGGGAGTTGGGGTTGGCGGCGAGGTCGAGACAGGAGATGAGGGACTTGAGGGAGGGGTCGCGGTCGATGAAGGCGGGGAGAAGGAGAGGAGAGGATATCGGTGGGCTGAGAAGATGGGCATGCCAAACATTTTACCATTCTACGGTCAAAATGGATCCATTAATGGAATACAAAGAGGCCTTAACTTCGGTTCAGCACATGCAACGATCTTGAATCACGGCAGCCAGAGTTATCAGTCTGTGAACCAGCAGCTCCGCCAAGTTTTTGATTCTATGCAACTCTTACAATTGCAGCTTAGCGAGGACAGAGCTCTCAGTTTCATCAAATCTTCCATCTTCTACCTCTCCTTTGGCAAAGATGACTTCATAGAGCTCTTCCTACGCAATGCCTCGGGTACTGCAAAACAGAATAGTACCGATCAAGAATTTTCACACATTTTAGCAGATCAGATGGTACATGTTATACGGAACCTTTATGACATGAATGTGAGGAGGATCATATGCATGGGAATATTGCCGTTGGGATGCACCCCGCGGGTGTTATTGAACCAGTACAATTCCTCCACAGCCCATCCAGATGAACATGATGATGATGATGTTGCAAGGGGATGTGTAGAGGAGATTAATGCACAGGTCTTCGAGTACAACAGAGAGCTGAATGAGCAAATCTCTGAGCTTCATGCAGAGTTGCCTGATGCACAGCTTCTGTTTTGTGATGTTTACCAAGGACTTACAACGATCATAAACAACCCAGAACAATATGGATTTGAGGATGTAAAGAATGCTTGCTGCGGGCTTGGTATTTATGGAGCAGAAATTGGATGTCTCTCTAAAGATATGGCGTGTGACAAAGAAGCAAGTCATGTGTGGTGGGATTTATACAATCCTACAAAGGCAGTGAACTCATTGCTGGCTGATTCAGCCTGGTCGAGCTATCCGCTTCGGATTTGTCGTCCTATGAGCATCCAGGAATTTTTTACCGCTTCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

396

Amino Acids

44.06

Weight (kDa)

5.14

Isoelectric Point (pI)

48.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 99 - 371 7.8e-17 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014790)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06121 FvH4_7g06190
malus_domestica MD00G1178200.v1.1
prunus_persica Prupe.6G152900_v2.0.a1
pyrus_communis pycom11g15560
rosa_chinensis RchiOBHm_Chr1g0333821
rosa_laevigata RLG00000029528
rosa_multiflora Rmu_sc0000026.1_g000017 Rmu_sc0007177.1_g000005
rosa_roxburghii Rroxscaffold_4G00317200
rosa_rugosa Rorug01G0112100
rosa_samantha Rh1AG134500 Rh1AG134600 Rh1BG102800 Rh1CG127900 Rh1DG140000
rosa_wichuraiana Rw1G011220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 42
AccII CGCG 2 cut(s) 257, 730
AclWI GGATC 4 cut(s) 202, 345, 358, 698
AcsI RAATTY 2 cut(s) 620, 1166
AcuI CTGAAG 1 cut(s) 1164
AfaI GTAC 5 cut(s) 592, 610, 651, 749, 839
AfiI CCNNNNNNNGG 1 cut(s) 338
AflIII ACRYGT 1 cut(s) 652
AgsI TTSAA 2 cut(s) 409, 742
AjnI CCWGG 2 cut(s) 1118, 1161
AjuI GAANNNNNNNTTGG 2 cut(s) 169, 201
AluBI AGCT 8 cut(s) 448, 490, 506, 568, 850, 871, 898, 1128
AluI AGCT 8 cut(s) 448, 490, 506, 568, 850, 871, 898, 1128
Alw21I GWGCWC 2 cut(s) 508, 570
Alw26I GTCTC 2 cut(s) 218, 1025
AlwI GGATC 4 cut(s) 202, 345, 358, 698
AlwNI CAGNNNCTG 1 cut(s) 901
Ama87I CYCGRG 1 cut(s) 586
AoxI GGCC 1 cut(s) 373
ApeKI GCWGC 5 cut(s) 86, 417, 445, 487, 984
ApoI RAATTY 2 cut(s) 620, 1166
AseI ATTAAT 2 cut(s) 357, 819
AsuHPI GGTGA 2 cut(s) 58, 179
AvaI CYCGRG 1 cut(s) 586
BamHI GGATCC 1 cut(s) 350
BanII GRGCYC 2 cut(s) 508, 570
BbsI GAAGAC 1 cut(s) 823
Bbv12I GWGCWC 2 cut(s) 508, 570
BbvI GCAGC 5 cut(s) 98, 429, 457, 499, 971
BccI CCATC 5 cut(s) 126, 304, 536, 640, 774
BceAI ACGGC 2 cut(s) 430, 697
BcgI CGANNNNNNTGC 2 cut(s) 78, 112
BciT130I CCWGG 2 cut(s) 1120, 1163
BcoDI GTCTC 2 cut(s) 218, 1025
BfuAI ACCTGC 1 cut(s) 42
BlpI GCTNAGC 1 cut(s) 491
Bme1390I CCNGG 2 cut(s) 1120, 1163
BmeT110I CYCGRG 1 cut(s) 586
BmiI GGNNCC 2 cut(s) 352, 665
BmrFI CCNGG 2 cut(s) 1120, 1163
BmsI GCATC 3 cut(s) 710, 880, 1167
BpiI GAAGAC 1 cut(s) 823
BplI GAGNNNNNCTC 2 cut(s) 848, 880
Bpu1102I GCTNAGC 1 cut(s) 491
BpuEI CTTGAG 1 cut(s) 263
BsaJI CCNNGG 3 cut(s) 585, 728, 919
BsaXI ACNNNNNCTCC 4 cut(s) 192, 222, 740, 770
Bsc4I CCNNNNNNNGG 1 cut(s) 338
Bse1I ACTGG 1 cut(s) 745
Bse3DI GCAATG 2 cut(s) 586, 1100
BseBI CCWGG 2 cut(s) 1120, 1163
BseDI CCNNGG 3 cut(s) 585, 728, 919
BseGI GGATG 7 cut(s) 137, 725, 766, 809, 973, 1022, 1158
BseLI CCNNNNNNNGG 1 cut(s) 338
BseMI GCAATG 2 cut(s) 586, 1100
BseMII CTCAG 3 cut(s) 294, 523, 858
BseNI ACTGG 1 cut(s) 745
BseRI GAGGAG 3 cut(s) 298, 700, 827
BseXI GCAGC 5 cut(s) 98, 429, 457, 499, 971
Bsh1236I CGCG 2 cut(s) 257, 730
Bsh1285I CGRYCG 1 cut(s) 261
BshFI GGCC 1 cut(s) 375
BsiEI CGRYCG 1 cut(s) 261
BsiHKAI GWGCWC 2 cut(s) 508, 570
BsiHKCI CYCGRG 1 cut(s) 586
BslFI GGGAC 1 cut(s) 252
BslI CCNNNNNNNGG 1 cut(s) 338
BsmAI GTCTC 2 cut(s) 218, 1025
BsmFI GGGAC 1 cut(s) 252
BsmI GAATGC 1 cut(s) 982
BsnI GGCC 1 cut(s) 375
BsoBI CYCGRG 1 cut(s) 586
Bsp1286I GDGCHC 2 cut(s) 508, 570
Bsp143I GATC 7 cut(s) 194, 350, 402, 613, 640, 690, 933
Bsp1720I GCTNAGC 1 cut(s) 491
BspANI GGCC 1 cut(s) 375
BspCNI CTCAG 3 cut(s) 295, 522, 859
BspFNI CGCG 2 cut(s) 257, 730
BspLI GGNNCC 2 cut(s) 352, 665
BspMI ACCTGC 1 cut(s) 42
BspPI GGATC 4 cut(s) 202, 345, 358, 698
BspQI GCTCTTC 1 cut(s) 575
BsrDI GCAATG 2 cut(s) 586, 1100
BsrI ACTGG 1 cut(s) 745
BssECI CCNNGG 3 cut(s) 585, 728, 919
BssMI GATC 7 cut(s) 194, 350, 402, 613, 640, 690, 933
BssT1I CCWWGG 1 cut(s) 919
Bst2UI CCWGG 2 cut(s) 1120, 1163
Bst4CI ACNGT 1 cut(s) 341
Bst6I CTCTTC 1 cut(s) 575
BstC8I GCNNGC 5 cut(s) 91, 317, 982, 989, 1107
BstDEI CTNAG 4 cut(s) 303, 491, 509, 867
BstDSI CCRYGG 1 cut(s) 728
BstF5I GGATG 7 cut(s) 137, 725, 766, 809, 973, 1022, 1158
BstFNI CGCG 2 cut(s) 257, 730
BstKTI GATC 7 cut(s) 197, 353, 405, 616, 643, 693, 936
BstMAI GTCTC 2 cut(s) 218, 1025
BstMBI GATC 7 cut(s) 194, 350, 402, 613, 640, 690, 933
BstMCI CGRYCG 1 cut(s) 261
BstMWI GCNNNNNNNGC 4 cut(s) 152, 155, 856, 1115
BstNI CCWGG 2 cut(s) 1120, 1163
BstNSI RCATGY 4 cut(s) 93, 319, 398, 656
BstSCI CCNGG 2 cut(s) 1118, 1161
BstUI CGCG 2 cut(s) 257, 730
BstV1I GCAGC 5 cut(s) 98, 429, 457, 499, 971
BstV2I GAAGAC 1 cut(s) 823
BstX2I RGATCY 1 cut(s) 350
BstYI RGATCY 1 cut(s) 350
BsuRI GGCC 1 cut(s) 375
BtgI CCRYGG 1 cut(s) 728
BtsCI GGATG 7 cut(s) 137, 725, 766, 809, 973, 1022, 1158
BtsI GCAGTG 1 cut(s) 1098
BtsIMutI CAGTG 1 cut(s) 1098
BveI ACCTGC 1 cut(s) 42
Cac8I GCNNGC 5 cut(s) 91, 317, 982, 989, 1107
CaiI CAGNNNCTG 1 cut(s) 901
Cfr42I CCGCGG 1 cut(s) 731
Csp6I GTAC 5 cut(s) 591, 609, 650, 748, 838
CviQI GTAC 5 cut(s) 591, 609, 650, 748, 838
DdeI CTNAG 4 cut(s) 303, 491, 509, 867
DpnI GATC 7 cut(s) 196, 352, 404, 615, 642, 692, 935
DpnII GATC 7 cut(s) 194, 350, 402, 613, 640, 690, 933
DraI TTTAAA 1 cut(s) 16
Eam1104I CTCTTC 1 cut(s) 575
EarI CTCTTC 1 cut(s) 575
EciI GGCGGA 1 cut(s) 440
Ecl136II GAGCTC 2 cut(s) 506, 568
Eco130I CCWWGG 1 cut(s) 919
Eco147I AGGCCT 1 cut(s) 375
Eco24I GRGCYC 2 cut(s) 508, 570
Eco32I GATATC 1 cut(s) 293
Eco53kI GAGCTC 2 cut(s) 506, 568
Eco57I CTGAAG 1 cut(s) 1164
Eco88I CYCGRG 1 cut(s) 586
EcoICRI GAGCTC 2 cut(s) 506, 568
EcoRII CCWGG 2 cut(s) 1118, 1161
EcoRV GATATC 1 cut(s) 293
EcoT14I CCWWGG 1 cut(s) 919
EcoT22I ATGCAT 1 cut(s) 701
EcoT38I GRGCYC 2 cut(s) 508, 570
ErhI CCWWGG 1 cut(s) 919
FaqI GGGAC 1 cut(s) 252
FauI CCCGC 4 cut(s) 263, 723, 735, 980
FauNDI CATATG 1 cut(s) 695
FokI GGATG 7 cut(s) 144, 732, 753, 816, 980, 1029, 1145
FriOI GRGCYC 2 cut(s) 508, 570
HaeIII GGCC 1 cut(s) 375
HinfI GANTC 3 cut(s) 409, 464, 1112
HphI GGTGA 2 cut(s) 58, 179
Hpy166II GTNNAC 3 cut(s) 439, 916, 1096
Hpy188I TCNGA 3 cut(s) 645, 868, 1140
Hpy188III TCNNGA 5 cut(s) 198, 223, 406, 617, 770
Hpy8I GTNNAC 3 cut(s) 439, 916, 1096
HpyAV CCTTC 2 cut(s) 260, 271
HpyCH4III ACNGT 1 cut(s) 341
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 155, 856, 1115
HpyF3I CTNAG 4 cut(s) 303, 491, 509, 867
KspI CCGCGG 1 cut(s) 731
Kzo9I GATC 7 cut(s) 194, 350, 402, 613, 640, 690, 933
LguI GCTCTTC 1 cut(s) 575
LmnI GCTCC 2 cut(s) 453, 1004
Lsp1109I GCAGC 5 cut(s) 98, 429, 457, 499, 971
LweI GCATC 3 cut(s) 710, 880, 1167
MaeIII GTNAC 1 cut(s) 1040
MalI GATC 7 cut(s) 196, 352, 404, 615, 642, 692, 935
MboI GATC 7 cut(s) 194, 350, 402, 613, 640, 690, 933
MboII GAAGA 6 cut(s) 88, 319, 516, 523, 562, 823
MfeI CAATTG 1 cut(s) 482
MflI RGATCY 1 cut(s) 350
MhlI GDGCHC 2 cut(s) 508, 570
MluCI AATT 6 cut(s) 34, 482, 620, 751, 1011, 1166
Mph1103I ATGCAT 1 cut(s) 701
MseI TTAA 6 cut(s) 15, 33, 357, 378, 819, 1186
MslI CAYNNNNRTG 4 cut(s) 680, 698, 771, 783
MspA1I CMGCKG 1 cut(s) 730
MspR9I CCNGG 2 cut(s) 1120, 1163
MunI CAATTG 1 cut(s) 482
Mva1269I GAATGC 1 cut(s) 982
MvaI CCWGG 2 cut(s) 1120, 1163
MvnI CGCG 2 cut(s) 257, 730
MwoI GCNNNNNNNGC 4 cut(s) 152, 155, 856, 1115
NdeI CATATG 1 cut(s) 695
NdeII GATC 7 cut(s) 194, 350, 402, 613, 640, 690, 933
NlaIV GGNNCC 2 cut(s) 352, 665
NmuCI GTSAC 1 cut(s) 1040
NsiI ATGCAT 1 cut(s) 701
NspI RCATGY 4 cut(s) 93, 319, 398, 656
PaeI GCATGC 2 cut(s) 93, 319
PceI AGGCCT 1 cut(s) 375
PciI ACATGT 1 cut(s) 652
PciSI GCTCTTC 1 cut(s) 575
PcsI WCGNNNNNNNCGW 1 cut(s) 1144
PctI GAATGC 1 cut(s) 982
PfeI GAWTC 3 cut(s) 409, 464, 1112
PfoI TCCNGGA 1 cut(s) 1161
PscI ACATGT 1 cut(s) 652
PshBI ATTAAT 2 cut(s) 357, 819
Psp124BI GAGCTC 2 cut(s) 508, 570
Psp6I CCWGG 2 cut(s) 1118, 1161
PspGI CCWGG 2 cut(s) 1118, 1161
PspN4I GGNNCC 2 cut(s) 352, 665
PstNI CAGNNNCTG 1 cut(s) 901
PsuI RGATCY 1 cut(s) 350
RsaI GTAC 5 cut(s) 592, 610, 651, 749, 839
RsaNI GTAC 5 cut(s) 591, 609, 650, 748, 838
RseI CAYNNNNRTG 4 cut(s) 680, 698, 771, 783
SacI GAGCTC 2 cut(s) 508, 570
SacII CCGCGG 1 cut(s) 731
SapI GCTCTTC 1 cut(s) 575
SaqAI TTAA 6 cut(s) 15, 33, 357, 378, 819, 1186
Sau3AI GATC 7 cut(s) 194, 350, 402, 613, 640, 690, 933
ScrFI CCNGG 2 cut(s) 1120, 1163
SduI GDGCHC 2 cut(s) 508, 570
SfaNI GCATC 3 cut(s) 710, 880, 1167
Sfr303I CCGCGG 1 cut(s) 731
SgrBI CCGCGG 1 cut(s) 731
SmiMI CAYNNNNRTG 4 cut(s) 680, 698, 771, 783
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
SphI GCATGC 2 cut(s) 93, 319
Sse9I AATT 6 cut(s) 34, 482, 620, 751, 1011, 1166
SseBI AGGCCT 1 cut(s) 375
SspI AATATT 2 cut(s) 82, 708
SstI GAGCTC 2 cut(s) 508, 570
StuI AGGCCT 1 cut(s) 375
StyD4I CCNGG 2 cut(s) 1118, 1161
StyI CCWWGG 1 cut(s) 919
TaaI ACNGT 1 cut(s) 341
TaqI TCGA 5 cut(s) 98, 222, 261, 834, 1124
TasI AATT 6 cut(s) 34, 482, 620, 751, 1011, 1166
TatI WGTACW 2 cut(s) 747, 837
TauI GCSGC 5 cut(s) 149, 152, 155, 158, 216
TfiI GAWTC 3 cut(s) 409, 464, 1112
Tru1I TTAA 6 cut(s) 15, 33, 357, 378, 819, 1186
Tru9I TTAA 6 cut(s) 15, 33, 357, 378, 819, 1186
TscAI CASTG 1 cut(s) 1098
TseFI GTSAC 1 cut(s) 1040
TseI GCWGC 5 cut(s) 86, 417, 445, 487, 984
Tsp45I GTSAC 1 cut(s) 1040
TspDTI ATGAA 7 cut(s) 36, 279, 505, 550, 692, 789, 863
TspGWI ACGGA 1 cut(s) 676
TspRI CASTG 1 cut(s) 1098
VspI ATTAAT 2 cut(s) 357, 819
XapI RAATTY 2 cut(s) 620, 1166
XceI RCATGY 4 cut(s) 93, 319, 398, 656
Zsp2I ATGCAT 1 cut(s) 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.