pycom11g15560

GDSL esterase lipase At1g71250-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0014790
Physical Location & Seq
Forward (+)
16296704 .. 16299656
2953 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g15560.2

Sequence Viewer

Length: 1473 bp
ATGGATGACGTCATAATATGTTGGCCCACCAATTTGAGACGGAAGCCTGGGCTCAAATTGCTCAGGCTTTTGGCTAGGCATGTAGCCCACGAATTGCCCTCCACAAAATGGATCGGTGGAACCGTTGAAGTAATTTTTGGGCAAAAGAGCAATTTGGCAGCCCTTTTGCGTGCCTTTTGGGTAACAACTGGACTTGCTCTAAGGATCTTTGCTCACTCGAACTCTTTTTTTTTTTCATGTGTGGCAGCGCAGAAGATAAGCGGGACGTTGAGCACAAGGAACAAAGCAAAGATGACGGGGTTGGATAGCGCGAAGCCGCCAAAAGGGTCGATTCTGGTTCATCTTCTTGCCCTCTCAGTGATGGGTTTCGTCGTTACAGCTCACGGGAAATTGCCTGAGGTTTCGGCAGTGCAGAATGACACCCCAAATGCGCCCCCTGCATTCTACATTCTTGGGGATTCCTCTGTCGACTGCGGAGACAACACGCTGTTTTACCCACTCGTTCACAGCTTTCTCTCCTTGTATTCCTGCAACGGTTCTTCTGATGCGAGTCTCATTCCTCATCTTCTCGCTGGGAAGATGGGGATGCCGAGTGTCCTACCATTCTACAGCCAACAAAACGGATCCATTGATGGAATACTAAGAGGCCTCAACTTTGGTTCGGCACAAGCAACTATCATGAACCCTAGCGGCCAGAGCTACCAGTCCATAAACCAACAACTCCGCCAAGTTTTCGATTCAATGCAGCTCTTACAACTGCAGCTCAGCCAGGATGTAGCTCTCCATTTCATCAGATCATCCATCTTCTACCTCTCCTTTGGCAAGGACGACTACATCAACCTCTACCTACGCCACGCCTCCTCCTCCTCCGGCGCTGAGAAACCCAAGAACAGTGACCAGGAATTTTCCCACATTTTAGCAAATCAGATGATACATGTCATAAGGAACCTGTACGACATGAATGTGAGGAGGATCATATGCATGGGGATATTGCCTTTGGGATGCACGCCGCGTATGTTGTTCGACCACCACAATTCCAGTTCCACCAATTCCAATTCTGAACCTGGTGACGAAGAAGACGATGATGTCCGGAGTGGATGCGTGGAGGAGATCAACGAGCAGGTCTCGGAGTACAACAAAGTGCTGCACCAGCAGATCGGCAAGCTCCACGCAGAGTTGCCGGATGCACATATAGTGTTCTGTGATGATGTGAAGAGTGCTTGCTGTGGGTTGGGCATGTACGGAGCAGCGATCGGATGCCTTTCGAAAGAAATGGCGTGCCAACAAGATTCAAGCCATGTGTGGTGGGATTTGTACAACCCAACCAAGGGAGTCAACTCCTTGCTTGCTGACTCCGCCTGGAACGGCTTTCCCCTCTCTCGCATTTGCCGCCCTACCACCATCAAGGAATTACTTTCCACCTCCACTACTACCGCCGCCGCCGCTGCTCCGGTCTCGCATCCTCCCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

491

Amino Acids

53.56

Weight (kDa)

6.64

Isoelectric Point (pI)

49.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014790)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06121 FvH4_7g06190
malus_domestica MD00G1178200.v1.1
prunus_persica Prupe.6G152900_v2.0.a1
pyrus_communis pycom11g15560
rosa_chinensis RchiOBHm_Chr1g0333821
rosa_laevigata RLG00000029528
rosa_multiflora Rmu_sc0000026.1_g000017 Rmu_sc0007177.1_g000005
rosa_roxburghii Rroxscaffold_4G00317200
rosa_rugosa Rorug01G0112100
rosa_samantha Rh1AG134500 Rh1AG134600 Rh1BG102800 Rh1CG127900 Rh1DG140000
rosa_wichuraiana Rw1G011220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1085
AatII GACGTC 1 cut(s) 12
Acc36I ACCTGC 1 cut(s) 1111
AccB7I CCANNNNNTGG 1 cut(s) 108
AccI GTMKAC 1 cut(s) 468
AccII CGCG 2 cut(s) 311, 1012
AccIII TCCGGA 1 cut(s) 1089
AclWI GGATC 5 cut(s) 119, 212, 618, 631, 980
AcoI YGGCCR 1 cut(s) 691
AcsI RAATTY 1 cut(s) 902
AcyI GRCGYC 1 cut(s) 9
AfaI GTAC 4 cut(s) 953, 1133, 1241, 1316
AfiI CCNNNNNNNGG 4 cut(s) 108, 323, 1327, 1328
AflIII ACRYGT 1 cut(s) 934
AgsI TTSAA 3 cut(s) 128, 741, 1293
AjnI CCWGG 5 cut(s) 46, 768, 897, 1063, 1358
AjuI GAANNNNNNNTTGG 2 cut(s) 120, 152
AluBI AGCT 7 cut(s) 380, 510, 699, 748, 763, 779, 1165
AluI AGCT 7 cut(s) 380, 510, 699, 748, 763, 779, 1165
Alw21I GWGCWC 1 cut(s) 275
Alw26I GTCTC 5 cut(s) 31, 471, 557, 1129, 1459
AlwI GGATC 5 cut(s) 119, 212, 618, 631, 980
Aor13HI TCCGGA 1 cut(s) 1089
AoxI GGCC 3 cut(s) 23, 646, 691
ApeKI GCWGC 7 cut(s) 158, 245, 745, 760, 1144, 1247, 1445
ApoI RAATTY 1 cut(s) 902
AspLEI GCGC 4 cut(s) 250, 311, 433, 875
AspS9I GGNCC 1 cut(s) 24
AsuHPI GGTGA 1 cut(s) 1079
AsuII TTCGAA 1 cut(s) 1265
AxyI CCTNAGG 1 cut(s) 396
BamHI GGATCC 1 cut(s) 623
BanII GRGCYC 1 cut(s) 54
BbsI GAAGAC 1 cut(s) 1083
Bbv12I GWGCWC 1 cut(s) 275
BbvI GCAGC 7 cut(s) 170, 257, 757, 772, 1131, 1259, 1432
BccI CCATC 5 cut(s) 355, 574, 626, 809, 1409
BceAI ACGGC 1 cut(s) 1381
BciT130I CCWGG 5 cut(s) 48, 770, 899, 1065, 1360
BcoDI GTCTC 5 cut(s) 31, 471, 557, 1129, 1459
BfaI CTAG 2 cut(s) 75, 687
BfmI CTRYAG 2 cut(s) 607, 758
BfoI RGCGCY 1 cut(s) 876
BfuAI ACCTGC 1 cut(s) 1111
BlpI GCTNAGC 1 cut(s) 764
Bme1390I CCNGG 5 cut(s) 48, 770, 899, 1065, 1360
BmgT120I GGNCC 1 cut(s) 24
BmiI GGNNCC 3 cut(s) 121, 625, 947
BmrFI CCNGG 5 cut(s) 48, 770, 899, 1065, 1360
BmsI GCATC 7 cut(s) 535, 576, 992, 1088, 1174, 1247, 1468
BpiI GAAGAC 1 cut(s) 1083
BplI GAGNNNNNCTC 2 cut(s) 1109, 1141
Bpu10I CCTNAGC 1 cut(s) 62
Bpu1102I GCTNAGC 1 cut(s) 764
Bpu14I TTCGAA 1 cut(s) 1265
BsaHI GRCGYC 1 cut(s) 9
BsaI GGTCTC 2 cut(s) 1129, 1459
BsaJI CCNNGG 2 cut(s) 47, 1326
BsaWI WCCGGW 2 cut(s) 1089, 1450
BsaXI ACNNNNNCTCC 2 cut(s) 845, 875
Bsc4I CCNNNNNNNGG 4 cut(s) 108, 323, 1327, 1328
Bse1I ACTGG 3 cut(s) 193, 703, 1038
Bse21I CCTNAGG 1 cut(s) 396
BseAI TCCGGA 1 cut(s) 1089
BseBI CCWGG 5 cut(s) 48, 770, 899, 1065, 1360
BseDI CCNNGG 2 cut(s) 47, 1326
BseGI GGATG 9 cut(s) 10, 591, 778, 797, 1007, 1103, 1189, 1262, 1459
BseLI CCNNNNNNNGG 4 cut(s) 108, 323, 1327, 1328
BseMII CTCAG 5 cut(s) 76, 369, 387, 778, 867
BseNI ACTGG 3 cut(s) 193, 703, 1038
BseRI GAGGAG 5 cut(s) 850, 853, 856, 982, 1121
BseXI GCAGC 7 cut(s) 170, 257, 757, 772, 1131, 1259, 1432
BseYI CCCAGC 1 cut(s) 572
BsgI GTGCAG 2 cut(s) 431, 1130
Bsh1236I CGCG 2 cut(s) 311, 1012
Bsh1285I CGRYCG 1 cut(s) 1254
BshFI GGCC 3 cut(s) 25, 648, 693
BsiEI CGRYCG 1 cut(s) 1254
BsiHKAI GWGCWC 1 cut(s) 275
BsiSI CCGG 4 cut(s) 870, 1090, 1181, 1451
BslFI GGGAC 1 cut(s) 277
BslI CCNNNNNNNGG 4 cut(s) 108, 323, 1327, 1328
BsmAI GTCTC 5 cut(s) 31, 471, 557, 1129, 1459
BsmBI CGTCTC 1 cut(s) 31
BsmFI GGGAC 1 cut(s) 277
BsmI GAATGC 1 cut(s) 440
BsnI GGCC 3 cut(s) 25, 648, 693
Bso31I GGTCTC 2 cut(s) 1129, 1459
Bsp119I TTCGAA 1 cut(s) 1265
Bsp1286I GDGCHC 2 cut(s) 54, 275
Bsp13I TCCGGA 1 cut(s) 1089
Bsp1407I TGTACA 1 cut(s) 1314
Bsp143I GATC 8 cut(s) 111, 204, 623, 794, 972, 1110, 1155, 1251
Bsp1720I GCTNAGC 1 cut(s) 764
BspANI GGCC 3 cut(s) 25, 648, 693
BspCNI CTCAG 5 cut(s) 75, 368, 388, 777, 868
BspEI TCCGGA 1 cut(s) 1089
BspFNI CGCG 2 cut(s) 311, 1012
BspHI TCATGA 1 cut(s) 678
BspLI GGNNCC 3 cut(s) 121, 625, 947
BspMAI CTGCAG 1 cut(s) 762
BspMI ACCTGC 1 cut(s) 1111
BspPI GGATC 5 cut(s) 119, 212, 618, 631, 980
BspT104I TTCGAA 1 cut(s) 1265
BspTNI GGTCTC 2 cut(s) 1129, 1459
BsrGI TGTACA 1 cut(s) 1314
BsrI ACTGG 3 cut(s) 193, 703, 1038
BssECI CCNNGG 2 cut(s) 47, 1326
BssMI GATC 8 cut(s) 111, 204, 623, 794, 972, 1110, 1155, 1251
BssNI GRCGYC 1 cut(s) 9
BssT1I CCWWGG 1 cut(s) 1326
Bst2UI CCWGG 5 cut(s) 48, 770, 899, 1065, 1360
Bst4CI ACNGT 3 cut(s) 124, 536, 893
Bst6I CTCTTC 1 cut(s) 1208
BstACI GRCGYC 1 cut(s) 9
BstAUI TGTACA 1 cut(s) 1314
BstBI TTCGAA 1 cut(s) 1265
BstC8I GCNNGC 6 cut(s) 171, 1007, 1163, 1222, 1279, 1347
BstDEI CTNAG 7 cut(s) 62, 200, 355, 396, 641, 764, 876
BstF5I GGATG 9 cut(s) 10, 591, 778, 797, 1007, 1103, 1189, 1262, 1459
BstFNI CGCG 2 cut(s) 311, 1012
BstH2I RGCGCY 1 cut(s) 876
BstHHI GCGC 4 cut(s) 250, 311, 433, 875
BstKTI GATC 8 cut(s) 114, 207, 626, 797, 975, 1113, 1158, 1254
BstMAI GTCTC 5 cut(s) 31, 471, 557, 1129, 1459
BstMBI GATC 8 cut(s) 111, 204, 623, 794, 972, 1110, 1155, 1251
BstMCI CGRYCG 1 cut(s) 1254
BstMWI GCNNNNNNNGC 8 cut(s) 58, 437, 696, 1150, 1355, 1389, 1442, 1445
BstNI CCWGG 5 cut(s) 48, 770, 899, 1065, 1360
BstNSI RCATGY 3 cut(s) 83, 938, 1240
BstSCI CCNGG 5 cut(s) 46, 768, 897, 1063, 1358
BstSFI CTRYAG 2 cut(s) 607, 758
BstUI CGCG 2 cut(s) 311, 1012
BstV1I GCAGC 7 cut(s) 170, 257, 757, 772, 1131, 1259, 1432
BstV2I GAAGAC 1 cut(s) 1083
BstX2I RGATCY 2 cut(s) 204, 623
BstYI RGATCY 2 cut(s) 204, 623
Bsu36I CCTNAGG 1 cut(s) 396
BsuRI GGCC 3 cut(s) 25, 648, 693
BtsCI GGATG 9 cut(s) 10, 591, 778, 797, 1007, 1103, 1189, 1262, 1459
BtsI GCAGTG 1 cut(s) 414
BtsIMutI CAGTG 3 cut(s) 363, 414, 898
BveI ACCTGC 1 cut(s) 1111
Cac8I GCNNGC 6 cut(s) 171, 1007, 1163, 1222, 1279, 1347
CciI TCATGA 1 cut(s) 678
CfoI GCGC 4 cut(s) 250, 311, 433, 875
Cfr13I GGNCC 1 cut(s) 24
CsiI ACCWGGT 1 cut(s) 1063
Csp6I GTAC 4 cut(s) 952, 1132, 1240, 1315
CviAII CATG 8 cut(s) 80, 237, 679, 935, 958, 982, 1237, 1298
CviQI GTAC 4 cut(s) 952, 1132, 1240, 1315
DdeI CTNAG 7 cut(s) 62, 200, 355, 396, 641, 764, 876
DpnI GATC 8 cut(s) 113, 206, 625, 796, 974, 1112, 1157, 1253
DpnII GATC 8 cut(s) 111, 204, 623, 794, 972, 1110, 1155, 1251
DrdI GACNNNNNNGTC 1 cut(s) 1085
DseDI GACNNNNNNGTC 1 cut(s) 1085
EaeI YGGCCR 1 cut(s) 691
Eam1104I CTCTTC 1 cut(s) 1208
EarI CTCTTC 1 cut(s) 1208
EciI GGCGGA 2 cut(s) 713, 1345
Eco130I CCWWGG 1 cut(s) 1326
Eco147I AGGCCT 1 cut(s) 648
Eco24I GRGCYC 1 cut(s) 54
Eco31I GGTCTC 2 cut(s) 1129, 1459
Eco81I CCTNAGG 1 cut(s) 396
EcoRII CCWGG 5 cut(s) 46, 768, 897, 1063, 1358
EcoT14I CCWWGG 1 cut(s) 1326
EcoT22I ATGCAT 1 cut(s) 983
EcoT38I GRGCYC 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 1326
Esp3I CGTCTC 1 cut(s) 31
FaeI CATG 8 cut(s) 83, 240, 682, 938, 961, 985, 1240, 1301
FaqI GGGAC 1 cut(s) 277
FatI CATG 8 cut(s) 79, 236, 678, 934, 957, 981, 1236, 1297
FauI CCCGC 1 cut(s) 254
FauNDI CATATG 1 cut(s) 977
FblI GTMKAC 1 cut(s) 468
FokI GGATG 9 cut(s) 17, 598, 784, 785, 1014, 1110, 1196, 1269, 1446
FriOI GRGCYC 1 cut(s) 54
FspBI CTAG 2 cut(s) 75, 687
GlaI GCGC 4 cut(s) 249, 310, 432, 874
GsaI CCCAGC 1 cut(s) 576
HaeII RGCGCY 1 cut(s) 876
HaeIII GGCC 3 cut(s) 25, 648, 693
HapII CCGG 4 cut(s) 870, 1090, 1181, 1451
HhaI GCGC 4 cut(s) 250, 311, 433, 875
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 8 cut(s) 83, 240, 682, 938, 961, 985, 1240, 1301
Hin6I GCGC 4 cut(s) 248, 309, 431, 873
HinP1I GCGC 4 cut(s) 248, 309, 431, 873
HincII GTYRAC 2 cut(s) 469, 1336
HindII GTYRAC 2 cut(s) 469, 1336
HinfI GANTC 7 cut(s) 331, 458, 550, 737, 1289, 1332, 1352
HpaII CCGG 4 cut(s) 870, 1090, 1181, 1451
HphI GGTGA 1 cut(s) 1079
Hpy166II GTNNAC 3 cut(s) 469, 505, 1336
Hpy188I TCNGA 6 cut(s) 544, 794, 927, 1060, 1129, 1256
Hpy188III TCNNGA 2 cut(s) 679, 1090
Hpy8I GTNNAC 3 cut(s) 469, 505, 1336
Hpy99I CGWCG 1 cut(s) 374
HpyCH4III ACNGT 3 cut(s) 124, 536, 893
HpyCH4IV ACGT 2 cut(s) 9, 266
HpyCH4V TGCA 9 cut(s) 412, 440, 531, 745, 760, 981, 1005, 1147, 1187
HpyF10VI GCNNNNNNNGC 8 cut(s) 58, 437, 696, 1150, 1355, 1389, 1442, 1445
HpyF3I CTNAG 7 cut(s) 62, 200, 355, 396, 641, 764, 876
HpySE526I ACGT 2 cut(s) 9, 266
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 8 cut(s) 83, 240, 682, 938, 961, 985, 1240, 1301
HspAI GCGC 4 cut(s) 248, 309, 431, 873
Kpn2I TCCGGA 1 cut(s) 1089
Kzo9I GATC 8 cut(s) 111, 204, 623, 794, 972, 1110, 1155, 1251
LmnI GCTCC 3 cut(s) 1170, 1244, 1453
Lsp1109I GCAGC 7 cut(s) 170, 257, 757, 772, 1131, 1259, 1432
LweI GCATC 7 cut(s) 535, 576, 992, 1088, 1174, 1247, 1468
MabI ACCWGGT 1 cut(s) 1063
MaeI CTAG 2 cut(s) 75, 687
MaeII ACGT 2 cut(s) 9, 266
MaeIII GTNAC 4 cut(s) 181, 373, 893, 1067
MalI GATC 8 cut(s) 113, 206, 625, 796, 974, 1112, 1157, 1253
MboI GATC 8 cut(s) 111, 204, 623, 794, 972, 1110, 1155, 1251
MboII GAAGA 9 cut(s) 265, 335, 531, 557, 589, 796, 1085, 1088, 1225
MflI RGATCY 2 cut(s) 204, 623
MhlI GDGCHC 2 cut(s) 54, 275
MlyI GAGTC 3 cut(s) 559, 1341, 1346
MmeI TCCRAC 1 cut(s) 282
Mph1103I ATGCAT 1 cut(s) 983
MroI TCCGGA 1 cut(s) 1089
MslI CAYNNNNRTG 2 cut(s) 962, 980
MspA1I CMGCKG 1 cut(s) 1445
MspI CCGG 4 cut(s) 870, 1090, 1181, 1451
MspR9I CCNGG 5 cut(s) 48, 770, 899, 1065, 1360
Mva1269I GAATGC 1 cut(s) 440
MvaI CCWGG 5 cut(s) 48, 770, 899, 1065, 1360
MvnI CGCG 2 cut(s) 311, 1012
MwoI GCNNNNNNNGC 8 cut(s) 58, 437, 696, 1150, 1355, 1389, 1442, 1445
NdeI CATATG 1 cut(s) 977
NdeII GATC 8 cut(s) 111, 204, 623, 794, 972, 1110, 1155, 1251
NlaIII CATG 8 cut(s) 83, 240, 682, 938, 961, 985, 1240, 1301
NlaIV GGNNCC 3 cut(s) 121, 625, 947
NmeAIII GCCGAG 1 cut(s) 615
NmuCI GTSAC 2 cut(s) 893, 1067
NsiI ATGCAT 1 cut(s) 983
NspI RCATGY 3 cut(s) 83, 938, 1240
NspV TTCGAA 1 cut(s) 1265
PagI TCATGA 1 cut(s) 678
PceI AGGCCT 1 cut(s) 648
PciI ACATGT 1 cut(s) 934
PcsI WCGNNNNNNNCGW 2 cut(s) 120, 1077
PctI GAATGC 1 cut(s) 440
PfeI GAWTC 4 cut(s) 331, 458, 737, 1289
PflMI CCANNNNNTGG 1 cut(s) 108
Ple19I CGATCG 1 cut(s) 1254
PleI GAGTC 3 cut(s) 558, 1340, 1346
PpsI GAGTC 3 cut(s) 558, 1340, 1346
PscI ACATGT 1 cut(s) 934
Psp6I CCWGG 5 cut(s) 46, 768, 897, 1063, 1358
PspFI CCCAGC 1 cut(s) 572
PspGI CCWGG 5 cut(s) 46, 768, 897, 1063, 1358
PspN4I GGNNCC 3 cut(s) 121, 625, 947
PspPI GGNCC 1 cut(s) 24
PstI CTGCAG 1 cut(s) 762
PsuI RGATCY 2 cut(s) 204, 623
PvuI CGATCG 1 cut(s) 1254
RsaI GTAC 4 cut(s) 953, 1133, 1241, 1316
RsaNI GTAC 4 cut(s) 952, 1132, 1240, 1315
RseI CAYNNNNRTG 2 cut(s) 962, 980
SalI GTCGAC 1 cut(s) 467
Sau3AI GATC 8 cut(s) 111, 204, 623, 794, 972, 1110, 1155, 1251
Sau96I GGNCC 1 cut(s) 24
SchI GAGTC 3 cut(s) 559, 1341, 1346
ScrFI CCNGG 5 cut(s) 48, 770, 899, 1065, 1360
SduI GDGCHC 2 cut(s) 54, 275
SexAI ACCWGGT 1 cut(s) 1063
SfaNI GCATC 7 cut(s) 535, 576, 992, 1088, 1174, 1247, 1468
SfcI CTRYAG 2 cut(s) 607, 758
SfuI TTCGAA 1 cut(s) 1265
SmiMI CAYNNNNRTG 2 cut(s) 962, 980
SseBI AGGCCT 1 cut(s) 648
SspMI CTAG 2 cut(s) 75, 687
StuI AGGCCT 1 cut(s) 648
StyD4I CCNGG 5 cut(s) 46, 768, 897, 1063, 1358
StyI CCWWGG 1 cut(s) 1326
TaaI ACNGT 3 cut(s) 124, 536, 893
TaiI ACGT 2 cut(s) 12, 269
TaqI TCGA 6 cut(s) 218, 329, 468, 735, 1023, 1265
TatI WGTACW 2 cut(s) 1131, 1314
TauI GCSGC 7 cut(s) 319, 693, 1012, 1392, 1439, 1442, 1445
TfiI GAWTC 4 cut(s) 331, 458, 737, 1289
TscAI CASTG 3 cut(s) 363, 414, 898
TseFI GTSAC 2 cut(s) 893, 1067
TseI GCWGC 7 cut(s) 158, 245, 745, 760, 1144, 1247, 1445
Tsp45I GTSAC 2 cut(s) 893, 1067
TspDTI ATGAA 5 cut(s) 225, 329, 695, 778, 974
TspGWI ACGGA 3 cut(s) 55, 636, 1257
TspRI CASTG 3 cut(s) 363, 414, 898
Van91I CCANNNNNTGG 1 cut(s) 108
XapI RAATTY 1 cut(s) 902
XceI RCATGY 3 cut(s) 83, 938, 1240
XmiI GTMKAC 1 cut(s) 468
XspI CTAG 2 cut(s) 75, 687
ZraI GACGTC 1 cut(s) 10
Zsp2I ATGCAT 1 cut(s) 983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.