Rh1AG134500

GDSL esterase lipase At1g71250-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0014790
Physical Location & Seq
Reverse (-)
25697361 .. 25698324
964 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG134500.1

Sequence Viewer

Length: 876 bp
ATGGGCATGCCAAACATTTTACCATTCTACAGTCAAAATGGATCCATTAATGGAATACAAAGAGGCCTTAACTTCGGTTCAGCACATGCAACGATCTTGAATCACGGCAGCCAGAGTTATCAGTCTGTGAACCAGCAGCTCCGCCAAGTTTTTGATTCTATGCAACTCTTACAATTGCAGCTTAGTGAGGACAGAGCTCTCAGTTTCATCAAATCTTCCATCTTCTACCTCTCCTTTGGCAAAGATGACTTCATAGAGCTCTTCCTACGCAATGCCTCAGGTACTGCAAAACAGAATAATACTGATCAAGAATTTTCACACATTTTAGCAGATCAGATGGTACATGTTATACGGAACCTTTATGACATGAATGTGAGGAGGATCATATGCATGGGAATATTGCCGTTGGGATGCACCCCGCGGGTGTTATTGAACCAGTACAATTCCACAGCCCATCCAAATGAAGATGATGATGATGATGTTCCAAGGGGATGTGTAGAGGAGATTAATGCACAGGTCTTAGAGTACAACAGAGAGCTGAATGGGCAAATCTCTGAGCTTCATGCAGAGTTGCCTGATGCACAGCTTCTGTTTTGTGATGTTTACCAAGGACTTGCAACGATCATAAACAACCCAGAACAATATGGATTTGAGGATGTAAAGAATGCTTGCTGCGGGCTTGGTATGTATGGAGCAGAAATTGGATGCCTCTCTAAAGATATGGCATGTGACAAAGAAGCAAGTCATGTGTGGTGGGATTTGTACAATCCTACAAAGGCAGTGAACTCATTGTTGGCTGATTCAGCCTGGTCGAGCTATCCGCTTCGGATTTGTCGTCCTATGAGCATCCAGGAATTTTTTAGTGCTTCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

291

Amino Acids

32.7

Weight (kDa)

4.74

Isoelectric Point (pI)

49.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 13 - 267 2e-15 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014790)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06121 FvH4_7g06190
malus_domestica MD00G1178200.v1.1
prunus_persica Prupe.6G152900_v2.0.a1
pyrus_communis pycom11g15560
rosa_chinensis RchiOBHm_Chr1g0333821
rosa_laevigata RLG00000029528
rosa_multiflora Rmu_sc0000026.1_g000017 Rmu_sc0007177.1_g000005
rosa_roxburghii Rroxscaffold_4G00317200
rosa_rugosa Rorug01G0112100
rosa_samantha Rh1AG134500 Rh1AG134600 Rh1BG102800 Rh1CG127900 Rh1DG140000
rosa_wichuraiana Rw1G011220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 421
AciI CCGC 5 cut(s) 142, 419, 421, 675, 821
AclWI GGATC 3 cut(s) 36, 49, 389
AcsI RAATTY 2 cut(s) 311, 854
AcuI CTGAAG 1 cut(s) 852
AfaI GTAC 5 cut(s) 283, 342, 440, 527, 764
AflIII ACRYGT 1 cut(s) 343
AgsI TTSAA 2 cut(s) 100, 433
AjnI CCWGG 2 cut(s) 806, 849
AjuI GAANNNNNNNTTGG 2 cut(s) 776, 808
AluBI AGCT 8 cut(s) 139, 181, 197, 259, 538, 559, 586, 816
AluI AGCT 8 cut(s) 139, 181, 197, 259, 538, 559, 586, 816
Alw21I GWGCWC 2 cut(s) 199, 261
AlwI GGATC 3 cut(s) 36, 49, 389
AlwNI CAGNNNCTG 2 cut(s) 284, 589
AoxI GGCC 1 cut(s) 64
ApeKI GCWGC 4 cut(s) 108, 136, 178, 672
ApoI RAATTY 2 cut(s) 311, 854
AseI ATTAAT 2 cut(s) 48, 507
AxyI CCTNAGG 1 cut(s) 277
BamHI GGATCC 1 cut(s) 41
BanII GRGCYC 2 cut(s) 199, 261
Bbv12I GWGCWC 2 cut(s) 199, 261
BbvI GCAGC 4 cut(s) 120, 148, 190, 659
BccI CCATC 3 cut(s) 227, 331, 462
BceAI ACGGC 2 cut(s) 121, 388
BciT130I CCWGG 2 cut(s) 808, 851
BclI TGATCA 1 cut(s) 304
BfmI CTRYAG 1 cut(s) 28
BisI GCNGC 4 cut(s) 109, 137, 179, 673
BlsI GCNGC 4 cut(s) 110, 138, 180, 674
Bme1390I CCNGG 2 cut(s) 808, 851
BmiI GGNNCC 2 cut(s) 43, 356
BmrFI CCNGG 2 cut(s) 808, 851
BmsI GCATC 4 cut(s) 401, 568, 695, 855
BsaJI CCNNGG 3 cut(s) 419, 485, 607
Bse1I ACTGG 1 cut(s) 436
Bse21I CCTNAGG 1 cut(s) 277
Bse3DI GCAATG 1 cut(s) 277
BseBI CCWGG 2 cut(s) 808, 851
BseDI CCNNGG 3 cut(s) 419, 485, 607
BseGI GGATG 6 cut(s) 416, 454, 497, 661, 710, 846
BseMI GCAATG 1 cut(s) 277
BseMII CTCAG 3 cut(s) 214, 291, 546
BseNI ACTGG 1 cut(s) 436
BseRI GAGGAG 2 cut(s) 391, 515
BseXI GCAGC 4 cut(s) 120, 148, 190, 659
Bsh1236I CGCG 1 cut(s) 421
BshFI GGCC 1 cut(s) 66
BsiHKAI GWGCWC 2 cut(s) 199, 261
BsmI GAATGC 1 cut(s) 670
BsnI GGCC 1 cut(s) 66
Bsp1286I GDGCHC 2 cut(s) 199, 261
Bsp1407I TGTACA 1 cut(s) 762
Bsp143I GATC 6 cut(s) 41, 93, 304, 331, 381, 621
BspACI CCGC 5 cut(s) 142, 419, 421, 675, 821
BspANI GGCC 1 cut(s) 66
BspCNI CTCAG 3 cut(s) 213, 290, 547
BspFNI CGCG 1 cut(s) 421
BspLI GGNNCC 2 cut(s) 43, 356
BspPI GGATC 3 cut(s) 36, 49, 389
BspQI GCTCTTC 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 277
BsrGI TGTACA 1 cut(s) 762
BsrI ACTGG 1 cut(s) 436
BssECI CCNNGG 3 cut(s) 419, 485, 607
BssMI GATC 6 cut(s) 41, 93, 304, 331, 381, 621
BssT1I CCWWGG 2 cut(s) 485, 607
Bst2UI CCWGG 2 cut(s) 808, 851
Bst4CI ACNGT 1 cut(s) 32
Bst6I CTCTTC 1 cut(s) 266
BstAUI TGTACA 1 cut(s) 762
BstC8I GCNNGC 3 cut(s) 8, 670, 677
BstDEI CTNAG 5 cut(s) 182, 200, 277, 520, 555
BstDSI CCRYGG 1 cut(s) 419
BstF5I GGATG 6 cut(s) 416, 454, 497, 661, 710, 846
BstFNI CGCG 1 cut(s) 421
BstKTI GATC 6 cut(s) 44, 96, 307, 334, 384, 624
BstMBI GATC 6 cut(s) 41, 93, 304, 331, 381, 621
BstMWI GCNNNNNNNGC 2 cut(s) 544, 803
BstNI CCWGG 2 cut(s) 808, 851
BstNSI RCATGY 4 cut(s) 10, 89, 347, 729
BstSCI CCNGG 2 cut(s) 806, 849
BstSFI CTRYAG 1 cut(s) 28
BstUI CGCG 1 cut(s) 421
BstV1I GCAGC 4 cut(s) 120, 148, 190, 659
BstX2I RGATCY 1 cut(s) 41
BstYI RGATCY 1 cut(s) 41
Bsu36I CCTNAGG 1 cut(s) 277
BsuRI GGCC 1 cut(s) 66
BtgI CCRYGG 1 cut(s) 419
BtsCI GGATG 6 cut(s) 416, 454, 497, 661, 710, 846
BtsI GCAGTG 1 cut(s) 786
BtsIMutI CAGTG 1 cut(s) 786
Cac8I GCNNGC 3 cut(s) 8, 670, 677
CaiI CAGNNNCTG 2 cut(s) 284, 589
Cfr42I CCGCGG 1 cut(s) 422
Csp6I GTAC 5 cut(s) 282, 341, 439, 526, 763
CviAII CATG 8 cut(s) 7, 86, 344, 367, 391, 563, 726, 746
CviQI GTAC 5 cut(s) 282, 341, 439, 526, 763
DdeI CTNAG 5 cut(s) 182, 200, 277, 520, 555
DpnI GATC 6 cut(s) 43, 95, 306, 333, 383, 623
DpnII GATC 6 cut(s) 41, 93, 304, 331, 381, 621
Eam1104I CTCTTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 266
EciI GGCGGA 1 cut(s) 131
Ecl136II GAGCTC 2 cut(s) 197, 259
Eco130I CCWWGG 2 cut(s) 485, 607
Eco147I AGGCCT 1 cut(s) 66
Eco24I GRGCYC 2 cut(s) 199, 261
Eco53kI GAGCTC 2 cut(s) 197, 259
Eco57I CTGAAG 1 cut(s) 852
Eco81I CCTNAGG 1 cut(s) 277
EcoICRI GAGCTC 2 cut(s) 197, 259
EcoRII CCWGG 2 cut(s) 806, 849
EcoT14I CCWWGG 2 cut(s) 485, 607
EcoT22I ATGCAT 1 cut(s) 392
EcoT38I GRGCYC 2 cut(s) 199, 261
ErhI CCWWGG 2 cut(s) 485, 607
FaeI CATG 8 cut(s) 10, 89, 347, 370, 394, 566, 729, 749
FatI CATG 8 cut(s) 6, 85, 343, 366, 390, 562, 725, 745
FauI CCCGC 3 cut(s) 414, 426, 668
FauNDI CATATG 1 cut(s) 386
FbaI TGATCA 1 cut(s) 304
Fnu4HI GCNGC 4 cut(s) 109, 137, 179, 673
FokI GGATG 6 cut(s) 423, 441, 504, 668, 717, 833
FriOI GRGCYC 2 cut(s) 199, 261
Fsp4HI GCNGC 4 cut(s) 109, 137, 179, 673
GluI GCNGC 4 cut(s) 109, 137, 179, 673
HaeIII GGCC 1 cut(s) 66
Hin1II CATG 8 cut(s) 10, 89, 347, 370, 394, 566, 729, 749
HinfI GANTC 3 cut(s) 100, 155, 800
Hpy166II GTNNAC 3 cut(s) 130, 604, 784
Hpy188I TCNGA 3 cut(s) 336, 556, 828
Hpy188III TCNNGA 2 cut(s) 97, 308
Hpy8I GTNNAC 3 cut(s) 130, 604, 784
HpyCH4III ACNGT 1 cut(s) 32
HpyF10VI GCNNNNNNNGC 2 cut(s) 544, 803
HpyF3I CTNAG 5 cut(s) 182, 200, 277, 520, 555
Hsp92II CATG 8 cut(s) 10, 89, 347, 370, 394, 566, 729, 749
Ksp22I TGATCA 1 cut(s) 304
KspI CCGCGG 1 cut(s) 422
Kzo9I GATC 6 cut(s) 41, 93, 304, 331, 381, 621
LguI GCTCTTC 1 cut(s) 266
LmnI GCTCC 2 cut(s) 144, 692
Lsp1109I GCAGC 4 cut(s) 120, 148, 190, 659
LweI GCATC 4 cut(s) 401, 568, 695, 855
MaeIII GTNAC 1 cut(s) 728
MalI GATC 6 cut(s) 43, 95, 306, 333, 383, 623
MboI GATC 6 cut(s) 41, 93, 304, 331, 381, 621
MboII GAAGA 4 cut(s) 207, 214, 253, 476
MfeI CAATTG 1 cut(s) 173
MflI RGATCY 1 cut(s) 41
MhlI GDGCHC 2 cut(s) 199, 261
MluCI AATT 5 cut(s) 173, 311, 442, 699, 854
MnlI CCTC 9 cut(s) 56, 181, 239, 286, 369, 372, 493, 646, 719
Mph1103I ATGCAT 1 cut(s) 392
MseI TTAA 4 cut(s) 48, 69, 507, 874
MslI CAYNNNNRTG 3 cut(s) 371, 389, 459
MspA1I CMGCKG 1 cut(s) 421
MspR9I CCNGG 2 cut(s) 808, 851
MunI CAATTG 1 cut(s) 173
Mva1269I GAATGC 1 cut(s) 670
MvaI CCWGG 2 cut(s) 808, 851
MvnI CGCG 1 cut(s) 421
MwoI GCNNNNNNNGC 2 cut(s) 544, 803
NdeI CATATG 1 cut(s) 386
NdeII GATC 6 cut(s) 41, 93, 304, 331, 381, 621
NlaIII CATG 8 cut(s) 10, 89, 347, 370, 394, 566, 729, 749
NlaIV GGNNCC 2 cut(s) 43, 356
NmuCI GTSAC 1 cut(s) 728
NsiI ATGCAT 1 cut(s) 392
NspI RCATGY 4 cut(s) 10, 89, 347, 729
PaeI GCATGC 1 cut(s) 10
PceI AGGCCT 1 cut(s) 66
PciI ACATGT 1 cut(s) 343
PciSI GCTCTTC 1 cut(s) 266
PcsI WCGNNNNNNNCGW 1 cut(s) 832
PctI GAATGC 1 cut(s) 670
PfeI GAWTC 3 cut(s) 100, 155, 800
PfoI TCCNGGA 1 cut(s) 849
PkrI GCNGC 4 cut(s) 110, 138, 180, 674
PscI ACATGT 1 cut(s) 343
PshBI ATTAAT 2 cut(s) 48, 507
Psp124BI GAGCTC 2 cut(s) 199, 261
Psp6I CCWGG 2 cut(s) 806, 849
PspGI CCWGG 2 cut(s) 806, 849
PspN4I GGNNCC 2 cut(s) 43, 356
PstNI CAGNNNCTG 2 cut(s) 284, 589
PsuI RGATCY 1 cut(s) 41
RsaI GTAC 5 cut(s) 283, 342, 440, 527, 764
RsaNI GTAC 5 cut(s) 282, 341, 439, 526, 763
RseI CAYNNNNRTG 3 cut(s) 371, 389, 459
SacI GAGCTC 2 cut(s) 199, 261
SacII CCGCGG 1 cut(s) 422
SapI GCTCTTC 1 cut(s) 266
SaqAI TTAA 4 cut(s) 48, 69, 507, 874
SatI GCNGC 4 cut(s) 109, 137, 179, 673
Sau3AI GATC 6 cut(s) 41, 93, 304, 331, 381, 621
ScrFI CCNGG 2 cut(s) 808, 851
SduI GDGCHC 2 cut(s) 199, 261
SfaNI GCATC 4 cut(s) 401, 568, 695, 855
SfcI CTRYAG 1 cut(s) 28
Sfr303I CCGCGG 1 cut(s) 422
SgrBI CCGCGG 1 cut(s) 422
SmiMI CAYNNNNRTG 3 cut(s) 371, 389, 459
SphI GCATGC 1 cut(s) 10
Sse9I AATT 5 cut(s) 173, 311, 442, 699, 854
SseBI AGGCCT 1 cut(s) 66
SsiI CCGC 5 cut(s) 142, 419, 421, 675, 821
SspI AATATT 1 cut(s) 399
SstI GAGCTC 2 cut(s) 199, 261
StuI AGGCCT 1 cut(s) 66
StyD4I CCNGG 2 cut(s) 806, 849
StyI CCWWGG 2 cut(s) 485, 607
TaaI ACNGT 1 cut(s) 32
TaqI TCGA 1 cut(s) 812
TasI AATT 5 cut(s) 173, 311, 442, 699, 854
TatI WGTACW 3 cut(s) 438, 525, 762
TfiI GAWTC 3 cut(s) 100, 155, 800
Tru1I TTAA 4 cut(s) 48, 69, 507, 874
Tru9I TTAA 4 cut(s) 48, 69, 507, 874
TscAI CASTG 1 cut(s) 786
TseFI GTSAC 1 cut(s) 728
TseI GCWGC 4 cut(s) 108, 136, 178, 672
Tsp45I GTSAC 1 cut(s) 728
TspDTI ATGAA 5 cut(s) 196, 241, 383, 477, 551
TspGWI ACGGA 1 cut(s) 367
TspRI CASTG 1 cut(s) 786
VspI ATTAAT 2 cut(s) 48, 507
XapI RAATTY 2 cut(s) 311, 854
XceI RCATGY 4 cut(s) 10, 89, 347, 729
Zsp2I ATGCAT 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.