FvH4_7g12260

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0016918
Physical Location & Seq
Reverse (-)
11104973 .. 11106979
2007 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g12260.t2

Sequence Viewer

Length: 576 bp
ATGGCTGGGACTAGAATGCTTCAATCATCTCCTACAAAGTTTTTCAAGGTTTTGATGCCTGGCTTCCGCAAGAAACTTGGTATTCCAGTAGCTTTCTGTCTCAATGAAAATAAAATGGATGCAGCGATGATACAAAGCTGCCAGGGATCTTGGGATGTTAAAGTTGGTAAGAGTGGTAATGGACAACTTTATTTCAAAGAAGGTTGGCTAAAATTCGTGAACCATCATAGCCTGAGAGTTGGAGAGTTTCTTGTATTTGAACACAAAGGGAATATGGTATTTAATGTGGTATCTTATGGTTTAAGTGCTTGTGAGAAAGAGTATCATTTCCCTTCTGATTCGATGGTAAACGGTAGGATGACAACACCTCATGAAACACAAAGGGTTAAGCGTCCTCGCGATGACAATTTGGAAGGAACTCATTTCTTCCGGACAATTATGAAGCCATCCCATCGTGATAAACGTAACCCTTATATGAATATCCCTCCTTCATTTGTTGAAGCGAATGGAATTGCTCACCAAATCCAGCATGACTCTGATAGATCCTTCAAGACATCATTGGAAGGTGGACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

192

Amino Acids

21.77

Weight (kDa)

9.51

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 45 - 114 1.7e-10 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 399
AccIII TCCGGA 1 cut(s) 429
AciI CCGC 1 cut(s) 67
AclWI GGATC 2 cut(s) 154, 537
AcsI RAATTY 1 cut(s) 212
AgsI TTSAA 6 cut(s) 23, 46, 196, 260, 500, 550
AjnI CCWGG 2 cut(s) 58, 141
AluBI AGCT 2 cut(s) 92, 138
AluI AGCT 2 cut(s) 92, 138
Alw26I GTCTC 1 cut(s) 104
AlwI GGATC 2 cut(s) 154, 537
Aor13HI TCCGGA 1 cut(s) 429
ApeKI GCWGC 2 cut(s) 122, 138
ApoI RAATTY 1 cut(s) 212
AspS9I GGNCC 1 cut(s) 569
AsuHPI GGTGA 1 cut(s) 509
AvaII GGWCC 1 cut(s) 569
BbvI GCAGC 2 cut(s) 125, 134
BccI CCATC 4 cut(s) 231, 337, 454, 459
BciT130I CCWGG 2 cut(s) 60, 143
BcoDI GTCTC 1 cut(s) 104
BfaI CTAG 1 cut(s) 12
BisI GCNGC 2 cut(s) 123, 139
BlsI GCNGC 2 cut(s) 124, 140
Bme1390I CCNGG 2 cut(s) 60, 143
Bme18I GGWCC 1 cut(s) 569
BmgT120I GGNCC 1 cut(s) 569
BmrFI CCNGG 2 cut(s) 60, 143
BmsI GCATC 2 cut(s) 45, 109
BsaJI CCNNGG 1 cut(s) 142
BsaWI WCCGGW 1 cut(s) 429
Bse1I ACTGG 1 cut(s) 86
BseAI TCCGGA 1 cut(s) 429
BseBI CCWGG 2 cut(s) 60, 143
BseDI CCNNGG 1 cut(s) 142
BseGI GGATG 4 cut(s) 124, 160, 363, 446
BseMII CTCAG 1 cut(s) 224
BseNI ACTGG 1 cut(s) 86
BseXI GCAGC 2 cut(s) 125, 134
BseYI CCCAGC 1 cut(s) 5
Bsh1236I CGCG 1 cut(s) 399
BsiSI CCGG 1 cut(s) 430
BslFI GGGAC 1 cut(s) 22
BsmAI GTCTC 1 cut(s) 104
BsmFI GGGAC 1 cut(s) 22
BsmI GAATGC 1 cut(s) 21
Bsp13I TCCGGA 1 cut(s) 429
Bsp143I GATC 2 cut(s) 146, 542
Bsp68I TCGCGA 1 cut(s) 399
BspACI CCGC 1 cut(s) 67
BspCNI CTCAG 1 cut(s) 225
BspEI TCCGGA 1 cut(s) 429
BspFNI CGCG 1 cut(s) 399
BspHI TCATGA 1 cut(s) 370
BspPI GGATC 2 cut(s) 154, 537
BsrI ACTGG 1 cut(s) 86
BssECI CCNNGG 1 cut(s) 142
BssMI GATC 2 cut(s) 146, 542
Bst2UI CCWGG 2 cut(s) 60, 143
Bst4CI ACNGT 1 cut(s) 353
BstDEI CTNAG 1 cut(s) 233
BstF5I GGATG 4 cut(s) 124, 160, 363, 446
BstFNI CGCG 1 cut(s) 399
BstKTI GATC 2 cut(s) 149, 545
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 2 cut(s) 146, 542
BstNI CCWGG 2 cut(s) 60, 143
BstSCI CCNGG 2 cut(s) 58, 141
BstUI CGCG 1 cut(s) 399
BstV1I GCAGC 2 cut(s) 125, 134
BstX2I RGATCY 2 cut(s) 146, 542
BstYI RGATCY 2 cut(s) 146, 542
BtgZI GCGATG 2 cut(s) 140, 414
BtsCI GGATG 4 cut(s) 124, 160, 363, 446
BtuMI TCGCGA 1 cut(s) 399
CciI TCATGA 1 cut(s) 370
Cfr13I GGNCC 1 cut(s) 569
CseI GACGC 1 cut(s) 380
CviAII CATG 2 cut(s) 371, 530
CviJI RGCY 7 cut(s) 5, 63, 92, 138, 208, 231, 445
CviKI_1 RGCY 7 cut(s) 5, 63, 92, 138, 208, 231, 445
DdeI CTNAG 1 cut(s) 233
DpnI GATC 2 cut(s) 148, 544
DpnII GATC 2 cut(s) 146, 542
Eco47I GGWCC 1 cut(s) 569
EcoRII CCWGG 2 cut(s) 58, 141
FaeI CATG 2 cut(s) 374, 533
FaiI YATR 8 cut(s) 228, 275, 297, 372, 440, 474, 476, 531
FaqI GGGAC 1 cut(s) 22
FatI CATG 2 cut(s) 370, 529
Fnu4HI GCNGC 2 cut(s) 123, 139
FokI GGATG 4 cut(s) 131, 167, 370, 433
Fsp4HI GCNGC 2 cut(s) 123, 139
FspBI CTAG 1 cut(s) 12
GluI GCNGC 2 cut(s) 123, 139
GsaI CCCAGC 1 cut(s) 9
HapII CCGG 1 cut(s) 430
HgaI GACGC 1 cut(s) 380
Hin1II CATG 2 cut(s) 374, 533
HinfI GANTC 2 cut(s) 338, 533
HpaII CCGG 1 cut(s) 430
HphI GGTGA 1 cut(s) 509
Hpy166II GTNNAC 3 cut(s) 220, 349, 569
Hpy188I TCNGA 2 cut(s) 337, 538
Hpy188III TCNNGA 6 cut(s) 217, 371, 398, 430, 455, 550
Hpy8I GTNNAC 3 cut(s) 220, 349, 569
HpyAV CCTTC 6 cut(s) 194, 342, 407, 498, 556, 557
HpyCH4III ACNGT 1 cut(s) 353
HpyCH4IV ACGT 1 cut(s) 463
HpyCH4V TGCA 1 cut(s) 122
HpyF3I CTNAG 1 cut(s) 233
HpySE526I ACGT 1 cut(s) 463
Hsp92II CATG 2 cut(s) 374, 533
Kpn2I TCCGGA 1 cut(s) 429
Kzo9I GATC 2 cut(s) 146, 542
LpnPI CCDG 8 cut(s) 45, 72, 99, 128, 155, 245, 443, 539
Lsp1109I GCAGC 2 cut(s) 125, 134
LweI GCATC 2 cut(s) 45, 109
MaeI CTAG 1 cut(s) 12
MaeII ACGT 1 cut(s) 463
MaeIII GTNAC 1 cut(s) 464
MalI GATC 2 cut(s) 148, 544
MboI GATC 2 cut(s) 146, 542
MboII GAAGA 1 cut(s) 418
MflI RGATCY 2 cut(s) 146, 542
MluCI AATT 4 cut(s) 212, 406, 435, 510
MlyI GAGTC 1 cut(s) 527
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 3 cut(s) 378, 405, 495
MroI TCCGGA 1 cut(s) 429
MseI TTAA 5 cut(s) 159, 282, 302, 387, 574
MspI CCGG 1 cut(s) 430
MspR9I CCNGG 2 cut(s) 60, 143
Mva1269I GAATGC 1 cut(s) 21
MvaI CCWGG 2 cut(s) 60, 143
MvnI CGCG 1 cut(s) 399
NdeII GATC 2 cut(s) 146, 542
NlaIII CATG 2 cut(s) 374, 533
NruI TCGCGA 1 cut(s) 399
PagI TCATGA 1 cut(s) 370
PcsI WCGNNNNNNNCGW 1 cut(s) 460
PctI GAATGC 1 cut(s) 21
PfeI GAWTC 1 cut(s) 338
PkrI GCNGC 2 cut(s) 124, 140
PleI GAGTC 1 cut(s) 527
PpsI GAGTC 1 cut(s) 527
Psp6I CCWGG 2 cut(s) 58, 141
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 2 cut(s) 58, 141
PspPI GGNCC 1 cut(s) 569
PsuI RGATCY 2 cut(s) 146, 542
RruI TCGCGA 1 cut(s) 399
SaqAI TTAA 5 cut(s) 159, 282, 302, 387, 574
SatI GCNGC 2 cut(s) 123, 139
Sau3AI GATC 2 cut(s) 146, 542
Sau96I GGNCC 1 cut(s) 569
SchI GAGTC 1 cut(s) 527
ScrFI CCNGG 2 cut(s) 60, 143
SetI ASST 8 cut(s) 51, 94, 140, 205, 370, 466, 568, 574
SfaNI GCATC 2 cut(s) 45, 109
SinI GGWCC 1 cut(s) 569
Sse9I AATT 4 cut(s) 212, 406, 435, 510
SsiI CCGC 1 cut(s) 67
SspMI CTAG 1 cut(s) 12
StyD4I CCNGG 2 cut(s) 58, 141
TaaI ACNGT 1 cut(s) 353
TaiI ACGT 1 cut(s) 466
TaqI TCGA 1 cut(s) 341
TasI AATT 4 cut(s) 212, 406, 435, 510
TfiI GAWTC 1 cut(s) 338
Tru1I TTAA 5 cut(s) 159, 282, 302, 387, 574
Tru9I TTAA 5 cut(s) 159, 282, 302, 387, 574
TseI GCWGC 2 cut(s) 122, 138
TspDTI ATGAA 5 cut(s) 120, 387, 455, 480, 491
VpaK11BI GGWCC 1 cut(s) 569
XapI RAATTY 1 cut(s) 212
XspI CTAG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.