Prupe.1G517200_v2.0.a1

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0016918
Physical Location & Seq
Forward (+)
42521528 .. 42525102
3575 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G517200.1

Sequence Viewer

Length: 732 bp
ATGGCTGGGAAATGCTCCGTGTTTTTCAAGTTTTTGGTTCCTGGGTTCAACAAAAGACTTTCACTTCCAGTAGCTTTCAGTAGTTCTCTGAGTGAAAAGAAACTGGATAAAGCAGTTATTAAAAGCTGCTTGGGGTCTTGGTGTGTGAGACTTGGCAGGAGTGTTGATGGAGTACTTTCTTTCGAAGAAGGTTGGGAAGTTTTTGTGAACCATCACGGCCTGAACATTGGAGAAATGGTAGTATTTGAACACAAAGGCAAAATGGTGTTCAATGTGGTTGCCTATGAGTCACTTGGTAGTGAGAAAGAGTATGAACTGCAGAGCGACAAGCACCAGCATGATTACAAAGGAAAAAGAACCTTAAAAGGAACTGCATCCTCAAGCTCAAGGACATTGTTCAGTACCACGATGAGTAAATCCCATGGGGATCCTCTTCATGCTTATATGACTTTTCCTGCGAAATTTGCAAGATCAAATGGCATTGTGGCCACATCCAGAATCATTCTCAAAGACCCTTCTGGAAGATCATGGCCGCTGATCATAAGTAAGTGGGAAAGCAAAACCAGGGGTAGTTATCGTATAGCTACTAGAAAAGGATGGTACAAGTTTTATGAAGCCAACAAGCTCAAAGATGGGGATGTCTGCATATTCAATCTCAAACCGGTATCATCTAAGTCAGGTTCAAAATCAACTCATGTCTTGGAAGTCCAGATAACCCGAGGTGGATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

244

Amino Acids

27.12

Weight (kDa)

9.78

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 533
AclWI GGATC 3 cut(s) 422, 435, 720
AcoI YGGCCR 2 cut(s) 486, 530
AcsI RAATTY 1 cut(s) 461
AfaI GTAC 3 cut(s) 174, 403, 602
AgeI ACCGGT 1 cut(s) 661
AgsI TTSAA 6 cut(s) 28, 49, 248, 271, 652, 684
AjnI CCWGG 2 cut(s) 40, 563
AluBI AGCT 5 cut(s) 74, 126, 384, 584, 625
AluI AGCT 5 cut(s) 74, 126, 384, 584, 625
Alw26I GTCTC 1 cut(s) 142
AlwI GGATC 3 cut(s) 422, 435, 720
Ama87I CYCGRG 1 cut(s) 717
AoxI GGCC 3 cut(s) 217, 486, 530
ApeKI GCWGC 1 cut(s) 126
ApoI RAATTY 1 cut(s) 461
AsiGI ACCGGT 1 cut(s) 661
AsuII TTCGAA 1 cut(s) 183
AvaI CYCGRG 1 cut(s) 717
BalI TGGCCA 1 cut(s) 488
BamHI GGATCC 2 cut(s) 427, 725
BbvI GCAGC 1 cut(s) 113
BccI CCATC 4 cut(s) 161, 219, 591, 626
BceAI ACGGC 1 cut(s) 232
BciT130I CCWGG 2 cut(s) 42, 565
BclI TGATCA 1 cut(s) 537
BcoDI GTCTC 1 cut(s) 142
BfaI CTAG 2 cut(s) 588, 730
BfmI CTRYAG 1 cut(s) 317
BisI GCNGC 2 cut(s) 127, 533
BlsI GCNGC 2 cut(s) 128, 534
BmcAI AGTACT 1 cut(s) 174
Bme1390I CCNGG 2 cut(s) 42, 565
BmeT110I CYCGRG 1 cut(s) 717
BmiI GGNNCC 3 cut(s) 39, 429, 727
BmrFI CCNGG 2 cut(s) 42, 565
BmsI GCATC 1 cut(s) 383
Bpu14I TTCGAA 1 cut(s) 183
BpuEI CTTGAG 2 cut(s) 364, 370
BsaJI CCNNGG 4 cut(s) 41, 421, 564, 718
BsaWI WCCGGW 1 cut(s) 661
BsaXI ACNNNNNCTCC 2 cut(s) 222, 252
Bse118I RCCGGY 1 cut(s) 661
Bse1I ACTGG 2 cut(s) 68, 108
BseBI CCWGG 2 cut(s) 42, 565
BseDI CCNNGG 4 cut(s) 41, 421, 564, 718
BseGI GGATG 4 cut(s) 374, 491, 602, 643
BseMII CTCAG 1 cut(s) 80
BseNI ACTGG 2 cut(s) 68, 108
BseXI GCAGC 1 cut(s) 113
BseYI CCCAGC 1 cut(s) 5
BshFI GGCC 3 cut(s) 219, 488, 532
BshTI ACCGGT 1 cut(s) 661
BsiHKCI CYCGRG 1 cut(s) 717
BsiSI CCGG 1 cut(s) 662
BsmAI GTCTC 1 cut(s) 142
BsnI GGCC 3 cut(s) 219, 488, 532
BsoBI CYCGRG 1 cut(s) 717
Bsp119I TTCGAA 1 cut(s) 183
Bsp143I GATC 5 cut(s) 427, 470, 524, 537, 725
Bsp19I CCATGG 1 cut(s) 421
BspACI CCGC 1 cut(s) 533
BspANI GGCC 3 cut(s) 219, 488, 532
BspCNI CTCAG 1 cut(s) 81
BspLI GGNNCC 3 cut(s) 39, 429, 727
BspMAI CTGCAG 1 cut(s) 321
BspPI GGATC 3 cut(s) 422, 435, 720
BspT104I TTCGAA 1 cut(s) 183
BsrFI RCCGGY 1 cut(s) 661
BsrI ACTGG 2 cut(s) 68, 108
BssAI RCCGGY 1 cut(s) 661
BssECI CCNNGG 4 cut(s) 41, 421, 564, 718
BssMI GATC 5 cut(s) 427, 470, 524, 537, 725
BssT1I CCWWGG 1 cut(s) 421
Bst2UI CCWGG 2 cut(s) 42, 565
Bst6I CTCTTC 1 cut(s) 438
BstBI TTCGAA 1 cut(s) 183
BstDEI CTNAG 2 cut(s) 89, 672
BstDSI CCRYGG 1 cut(s) 421
BstF5I GGATG 4 cut(s) 374, 491, 602, 643
BstKTI GATC 5 cut(s) 430, 473, 527, 540, 728
BstMAI GTCTC 1 cut(s) 142
BstMBI GATC 5 cut(s) 427, 470, 524, 537, 725
BstMWI GCNNNNNNNGC 1 cut(s) 464
BstNI CCWGG 2 cut(s) 42, 565
BstSCI CCNGG 2 cut(s) 40, 563
BstSFI CTRYAG 1 cut(s) 317
BstV1I GCAGC 1 cut(s) 113
BstX2I RGATCY 2 cut(s) 427, 725
BstYI RGATCY 2 cut(s) 427, 725
BsuRI GGCC 3 cut(s) 219, 488, 532
BtgI CCRYGG 1 cut(s) 421
BtsCI GGATG 4 cut(s) 374, 491, 602, 643
Cfr10I RCCGGY 1 cut(s) 661
Csp6I GTAC 3 cut(s) 173, 402, 601
CspAI ACCGGT 1 cut(s) 661
CviAII CATG 5 cut(s) 338, 422, 437, 528, 695
CviQI GTAC 3 cut(s) 173, 402, 601
DdeI CTNAG 2 cut(s) 89, 672
DpnI GATC 5 cut(s) 429, 472, 526, 539, 727
DpnII GATC 5 cut(s) 427, 470, 524, 537, 725
EaeI YGGCCR 2 cut(s) 486, 530
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Eco130I CCWWGG 1 cut(s) 421
Eco88I CYCGRG 1 cut(s) 717
EcoRII CCWGG 2 cut(s) 40, 563
EcoT14I CCWWGG 1 cut(s) 421
ErhI CCWWGG 1 cut(s) 421
FaeI CATG 5 cut(s) 341, 425, 440, 531, 698
FatI CATG 5 cut(s) 337, 421, 436, 527, 694
FbaI TGATCA 1 cut(s) 537
Fnu4HI GCNGC 2 cut(s) 127, 533
FokI GGATG 4 cut(s) 361, 478, 609, 650
Fsp4HI GCNGC 2 cut(s) 127, 533
FspBI CTAG 2 cut(s) 588, 730
GluI GCNGC 2 cut(s) 127, 533
GsaI CCCAGC 1 cut(s) 9
HaeIII GGCC 3 cut(s) 219, 488, 532
HapII CCGG 1 cut(s) 662
Hin1II CATG 5 cut(s) 341, 425, 440, 531, 698
HinfI GANTC 2 cut(s) 287, 498
HpaII CCGG 1 cut(s) 662
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 1 cut(s) 90
Hpy188III TCNNGA 3 cut(s) 495, 519, 709
Hpy8I GTNNAC 1 cut(s) 208
HpyAV CCTTC 2 cut(s) 182, 525
HpyCH4V TGCA 4 cut(s) 319, 374, 467, 645
HpyF10VI GCNNNNNNNGC 1 cut(s) 464
HpyF3I CTNAG 2 cut(s) 89, 672
Hsp92II CATG 5 cut(s) 341, 425, 440, 531, 698
Ksp22I TGATCA 1 cut(s) 537
Kzo9I GATC 5 cut(s) 427, 470, 524, 537, 725
LmnI GCTCC 1 cut(s) 20
Lsp1109I GCAGC 1 cut(s) 113
LweI GCATC 1 cut(s) 383
MaeI CTAG 2 cut(s) 588, 730
MaeIII GTNAC 1 cut(s) 288
MalI GATC 5 cut(s) 429, 472, 526, 539, 727
MboI GATC 5 cut(s) 427, 470, 524, 537, 725
MboII GAAGA 3 cut(s) 197, 425, 534
MflI RGATCY 2 cut(s) 427, 725
MlsI TGGCCA 1 cut(s) 488
MluCI AATT 1 cut(s) 461
MluNI TGGCCA 1 cut(s) 488
MlyI GAGTC 1 cut(s) 296
MnlI CCTC 3 cut(s) 388, 441, 713
Mox20I TGGCCA 1 cut(s) 488
MscI TGGCCA 1 cut(s) 488
MseI TTAA 2 cut(s) 120, 362
MslI CAYNNNNRTG 1 cut(s) 336
Msp20I TGGCCA 1 cut(s) 488
MspA1I CMGCKG 1 cut(s) 535
MspI CCGG 1 cut(s) 662
MspR9I CCNGG 2 cut(s) 42, 565
MvaI CCWGG 2 cut(s) 42, 565
MwoI GCNNNNNNNGC 1 cut(s) 464
NcoI CCATGG 1 cut(s) 421
NdeII GATC 5 cut(s) 427, 470, 524, 537, 725
NlaIII CATG 5 cut(s) 341, 425, 440, 531, 698
NlaIV GGNNCC 3 cut(s) 39, 429, 727
NmuCI GTSAC 1 cut(s) 288
NspV TTCGAA 1 cut(s) 183
PfeI GAWTC 1 cut(s) 498
PinAI ACCGGT 1 cut(s) 661
PkrI GCNGC 2 cut(s) 128, 534
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
Psp6I CCWGG 2 cut(s) 40, 563
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 2 cut(s) 40, 563
PspN4I GGNNCC 3 cut(s) 39, 429, 727
PstI CTGCAG 1 cut(s) 321
PsuI RGATCY 2 cut(s) 427, 725
RsaI GTAC 3 cut(s) 174, 403, 602
RsaNI GTAC 3 cut(s) 173, 402, 601
RseI CAYNNNNRTG 1 cut(s) 336
SaqAI TTAA 2 cut(s) 120, 362
SatI GCNGC 2 cut(s) 127, 533
Sau3AI GATC 5 cut(s) 427, 470, 524, 537, 725
ScaI AGTACT 1 cut(s) 174
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 2 cut(s) 42, 565
SetI ASST 9 cut(s) 76, 128, 193, 362, 386, 586, 627, 682, 724
SfaNI GCATC 1 cut(s) 383
SfcI CTRYAG 1 cut(s) 317
SfuI TTCGAA 1 cut(s) 183
SmiMI CAYNNNNRTG 1 cut(s) 336
SmlI CTYRAG 2 cut(s) 379, 385
SmoI CTYRAG 2 cut(s) 379, 385
Sse9I AATT 1 cut(s) 461
SsiI CCGC 1 cut(s) 533
SspMI CTAG 2 cut(s) 588, 730
StyD4I CCNGG 2 cut(s) 40, 563
StyI CCWWGG 1 cut(s) 421
TaqI TCGA 1 cut(s) 183
TasI AATT 1 cut(s) 461
TatI WGTACW 1 cut(s) 172
TauI GCSGC 1 cut(s) 535
TfiI GAWTC 1 cut(s) 498
Tru1I TTAA 2 cut(s) 120, 362
Tru9I TTAA 2 cut(s) 120, 362
TseFI GTSAC 1 cut(s) 288
TseI GCWGC 1 cut(s) 126
Tsp45I GTSAC 1 cut(s) 288
TspDTI ATGAA 3 cut(s) 327, 425, 627
TspGWI ACGGA 1 cut(s) 7
XapI RAATTY 1 cut(s) 461
XspI CTAG 2 cut(s) 588, 730
ZrmI AGTACT 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.