FvH4_7g23780

Proline transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006135
Physical Location & Seq
Forward (+)
18485988 .. 18489552
3565 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g23780.t1

Sequence Viewer

Length: 1317 bp
ATGAACATCACTGAAGCCGATACCAAAGTCTACGACGAAGACAGTGTGGGCCTTGACGTTCCGGAAACGGCCCATCAGATTAGCACAGATTCATGGTTTCAAGCGGGGTTTGTGCTGACGACTGGAATCAACAGTGCGTATGTGCTGGGGTATTCCGGGACTGTGATGGTGCCGCTGAGTTGGGTAGGTGGTGTGGTGGGGTTGATTTTAGCCACTGCTATATCACTCTACGCTAACACTCTGATTGCCAAGCTTCATTTACTTGGAGGGAAGAGGCACATCAGATATAGGGATCTTGCCGGGTTTATATACGGGAAGAAGGCTTATGCGCTTACATGGGCATTTCAGTATGTGAATCTTTTCATGATCAACACTGGATTTATTATTTTGGCTGGTTCTGCCCTCAAGGCTGCCTATCTTCTGTTCCGGGATGACAGTGTTATGAAACTGCCGTACTTTATTGCCATAGCGGGTTTTGTGTGCGCATTATTTGCCATTGGGATACCACACTTGTCAGCACTGAGGGCTTGGCTGGGGGTTTCAACAGTCTTGAGCTTGGTCTATATTATTGTGGCACTGGTCTTGTCGATTAAAGATGGAATCAAAGCACCACCCAGGGATTATGGCGTCCCAGGGACACGTATAGGCAAAATATTTACAACAACTGGAGCAGTTGCTAACCTTGTTTTCGCATTCAATACTGGAATGCTTCCGGAGATACAGGCTACAGTGAGGAAGCCAGCGGTTGGAAACATGCTAAAAGCTCTGTACTTTCAGTTCACAGCTGGTGTTTTGCCAATGTACGCAGTAACTTTTGTAGGGTATTGGGCATACGGAAACTCAACATCAGCTTACTTGCTCAGCAACGTAAACGGACCAGTTTGGGTGAAGGCATTTGCTAATATCTCAGCCTTCCTGCAATCAGTCATTGCTTTGCATATATTTGCAAGTCCAATGTATGAGTATTTGGACACAAAGTTTGGGATTAAAGGAAGTCCATTCAAAATCCAGAACTTGACATTCAGAATTACCGTTAGAGGCGGCTATCTAGCTGTAAACACATTTGTTGCAGCTCTACTGCCATTCCTTGGAGATTTCATGAGTCTCACAGGAGCCATCAGCACATTCCCTCTTACGTTCATTCTCGCAAACCACATGTACCTGGTGGCAAAGAAGGACAAACTCACTTCATCACAAAAGCTCTGGCATTGGCTAAATATCTGCGTCTTTAGCTTCTTGGCAGTTGCAGCCCTGATATCGGCCTTGAGGCTCATTGCAGTTCATTCCACACAGTACCATGTCTTTGCTGATCTATGA

Protein Analysis

439

Amino Acids

47.8

Weight (kDa)

9.46

Isoelectric Point (pI)

25.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 29 - 422 9.6e-85 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 484
AccB1I GGYRCC 1 cut(s) 169
AccB7I CCANNNNNTGG 2 cut(s) 746, 1088
AccI GTMKAC 1 cut(s) 30
AccIII TCCGGA 2 cut(s) 61, 712
AciI CCGC 5 cut(s) 104, 173, 470, 743, 1041
AclWI GGATC 1 cut(s) 300
AcuI CTGAAG 1 cut(s) 33
AcyI GRCGYC 1 cut(s) 627
AfaI GTAC 5 cut(s) 455, 770, 803, 1160, 1295
AfiI CCNNNNNNNGG 3 cut(s) 746, 1088, 1258
AflIII ACRYGT 2 cut(s) 638, 1155
AgsI TTSAA 4 cut(s) 101, 543, 697, 1003
AjnI CCWGG 3 cut(s) 614, 631, 1161
AluBI AGCT 9 cut(s) 253, 555, 764, 785, 851, 1052, 1073, 1201, 1233
AluI AGCT 9 cut(s) 253, 555, 764, 785, 851, 1052, 1073, 1201, 1233
Alw26I GTCTC 1 cut(s) 1109
AlwI GGATC 1 cut(s) 300
Aor13HI TCCGGA 2 cut(s) 61, 712
AoxI GGCC 3 cut(s) 49, 69, 1260
ApeKI GCWGC 3 cut(s) 410, 1070, 1247
ArsI GACNNNNNNTTYG 2 cut(s) 962, 994
Asp700I GAANNNNTTC 1 cut(s) 359
AspLEI GCGC 2 cut(s) 331, 485
AspS9I GGNCC 3 cut(s) 49, 70, 875
AsuC2I CCSGG 3 cut(s) 157, 301, 428
AsuHPI GGTGA 1 cut(s) 898
AvaII GGWCC 1 cut(s) 875
BaeI ACNNNNGTAYC 2 cut(s) 12, 45
BanI GGYRCC 1 cut(s) 169
BbsI GAAGAC 1 cut(s) 45
BbvI GCAGC 3 cut(s) 397, 1082, 1259
BccI CCATC 4 cut(s) 81, 160, 590, 1124
BceAI ACGGC 2 cut(s) 84, 436
BciT130I CCWGG 3 cut(s) 616, 633, 1163
BciVI GTATCC 1 cut(s) 495
BclI TGATCA 1 cut(s) 366
BcnI CCSGG 3 cut(s) 157, 301, 428
BcoDI GTCTC 1 cut(s) 1109
BfaI CTAG 1 cut(s) 1049
BfmI CTRYAG 1 cut(s) 726
BfuI GTATCC 1 cut(s) 495
BglI GCCNNNNNGGC 1 cut(s) 407
BisI GCNGC 5 cut(s) 173, 411, 1042, 1071, 1248
BlpI GCTNAGC 1 cut(s) 860
BlsI GCNGC 5 cut(s) 174, 412, 1043, 1072, 1249
Bme1390I CCNGG 6 cut(s) 157, 301, 428, 616, 633, 1163
Bme18I GGWCC 1 cut(s) 875
BmgT120I GGNCC 3 cut(s) 49, 70, 875
BmiI GGNNCC 2 cut(s) 171, 1114
BmrFI CCNGG 6 cut(s) 157, 301, 428, 616, 633, 1163
BpiI GAAGAC 1 cut(s) 45
BpmI CTGGAG 1 cut(s) 687
Bpu1102I GCTNAGC 1 cut(s) 860
BpuEI CTTGAG 3 cut(s) 389, 571, 1285
BpuMI CCSGG 3 cut(s) 157, 301, 428
BsaAI YACGTR 1 cut(s) 641
BsaHI GRCGYC 1 cut(s) 627
BsaJI CCNNGG 5 cut(s) 614, 615, 631, 632, 1087
BsaWI WCCGGW 2 cut(s) 61, 712
Bsc4I CCNNNNNNNGG 3 cut(s) 746, 1088, 1258
Bse1I ACTGG 6 cut(s) 127, 379, 582, 670, 706, 878
Bse3DI GCAATG 2 cut(s) 927, 1272
BseAI TCCGGA 2 cut(s) 61, 712
BseBI CCWGG 3 cut(s) 616, 633, 1163
BseDI CCNNGG 5 cut(s) 614, 615, 631, 632, 1087
BseGI GGATG 1 cut(s) 436
BseLI CCNNNNNNNGG 3 cut(s) 746, 1088, 1258
BseMI GCAATG 2 cut(s) 927, 1272
BseMII CTCAG 4 cut(s) 167, 512, 874, 921
BseNI ACTGG 6 cut(s) 127, 379, 582, 670, 706, 878
BseXI GCAGC 3 cut(s) 397, 1082, 1259
BseYI CCCAGC 2 cut(s) 145, 532
BshFI GGCC 3 cut(s) 51, 71, 1262
BshNI GGYRCC 1 cut(s) 169
BsiSI CCGG 5 cut(s) 62, 156, 300, 427, 713
BslFI GGGAC 3 cut(s) 172, 614, 649
BslI CCNNNNNNNGG 3 cut(s) 746, 1088, 1258
BsmAI GTCTC 1 cut(s) 1109
BsmFI GGGAC 3 cut(s) 172, 614, 649
BsmI GAATGC 2 cut(s) 692, 711
BsnI GGCC 3 cut(s) 51, 71, 1262
Bsp13I TCCGGA 2 cut(s) 61, 712
Bsp143I GATC 3 cut(s) 292, 366, 1309
Bsp1720I GCTNAGC 1 cut(s) 860
BspACI CCGC 5 cut(s) 104, 173, 470, 743, 1041
BspANI GGCC 3 cut(s) 51, 71, 1262
BspCNI CTCAG 4 cut(s) 168, 513, 873, 920
BspEI TCCGGA 2 cut(s) 61, 712
BspHI TCATGA 2 cut(s) 363, 1098
BspLI GGNNCC 2 cut(s) 171, 1114
BspPI GGATC 1 cut(s) 300
BspT107I GGYRCC 1 cut(s) 169
BsrDI GCAATG 2 cut(s) 927, 1272
BsrI ACTGG 6 cut(s) 127, 379, 582, 670, 706, 878
BssECI CCNNGG 5 cut(s) 614, 615, 631, 632, 1087
BssMI GATC 3 cut(s) 292, 366, 1309
BssNI GRCGYC 1 cut(s) 627
BssT1I CCWWGG 1 cut(s) 1087
Bst2UI CCWGG 3 cut(s) 616, 633, 1163
Bst4CI ACNGT 8 cut(s) 44, 134, 163, 437, 547, 730, 1033, 1293
Bst6I CTCTTC 1 cut(s) 266
BstACI GRCGYC 1 cut(s) 627
BstAPI GCANNNNNTGC 1 cut(s) 491
BstBAI YACGTR 1 cut(s) 641
BstC8I GCNNGC 1 cut(s) 741
BstDEI CTNAG 4 cut(s) 176, 521, 860, 907
BstF5I GGATG 1 cut(s) 436
BstHHI GCGC 2 cut(s) 331, 485
BstKTI GATC 3 cut(s) 295, 369, 1312
BstMAI GTCTC 1 cut(s) 1109
BstMBI GATC 3 cut(s) 292, 366, 1309
BstMWI GCNNNNNNNGC 6 cut(s) 398, 407, 491, 524, 1230, 1247
BstNI CCWGG 3 cut(s) 616, 633, 1163
BstNSI RCATGY 2 cut(s) 757, 1159
BstSCI CCNGG 6 cut(s) 155, 299, 426, 614, 631, 1161
BstSFI CTRYAG 1 cut(s) 726
BstV1I GCAGC 3 cut(s) 397, 1082, 1259
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
BsuI GTATCC 1 cut(s) 495
BsuRI GGCC 3 cut(s) 51, 71, 1262
BtsCI GGATG 1 cut(s) 436
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 9 cut(s) 9, 49, 139, 213, 372, 442, 518, 575, 735
Cac8I GCNNGC 1 cut(s) 741
CciI TCATGA 2 cut(s) 363, 1098
CfoI GCGC 2 cut(s) 331, 485
Cfr13I GGNCC 3 cut(s) 49, 70, 875
CseI GACGC 2 cut(s) 616, 1213
CsiI ACCWGGT 1 cut(s) 1161
Csp6I GTAC 5 cut(s) 454, 769, 802, 1159, 1294
CviAII CATG 7 cut(s) 93, 336, 364, 754, 1099, 1156, 1298
CviQI GTAC 5 cut(s) 454, 769, 802, 1159, 1294
DdeI CTNAG 4 cut(s) 176, 521, 860, 907
DpnI GATC 3 cut(s) 294, 368, 1311
DpnII GATC 3 cut(s) 292, 366, 1309
Eam1104I CTCTTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 266
Eco130I CCWWGG 1 cut(s) 1087
Eco32I GATATC 1 cut(s) 1257
Eco47I GGWCC 1 cut(s) 875
Eco57I CTGAAG 1 cut(s) 33
EcoRII CCWGG 3 cut(s) 614, 631, 1161
EcoRV GATATC 1 cut(s) 1257
EcoT14I CCWWGG 1 cut(s) 1087
ErhI CCWWGG 1 cut(s) 1087
FaeI CATG 7 cut(s) 96, 339, 367, 757, 1102, 1159, 1301
FaqI GGGAC 3 cut(s) 172, 614, 649
FatI CATG 7 cut(s) 92, 335, 363, 753, 1098, 1155, 1297
FauI CCCGC 2 cut(s) 97, 463
FbaI TGATCA 1 cut(s) 366
FblI GTMKAC 1 cut(s) 30
Fnu4HI GCNGC 5 cut(s) 173, 411, 1042, 1071, 1248
FokI GGATG 1 cut(s) 443
Fsp4HI GCNGC 5 cut(s) 173, 411, 1042, 1071, 1248
FspAI RTGCGCAY 1 cut(s) 484
FspBI CTAG 1 cut(s) 1049
FspI TGCGCA 1 cut(s) 484
GlaI GCGC 2 cut(s) 330, 484
GluI GCNGC 5 cut(s) 173, 411, 1042, 1071, 1248
GsaI CCCAGC 2 cut(s) 149, 536
GsuI CTGGAG 1 cut(s) 687
HaeIII GGCC 3 cut(s) 51, 71, 1262
HapII CCGG 5 cut(s) 62, 156, 300, 427, 713
HgaI GACGC 2 cut(s) 616, 1213
HhaI GCGC 2 cut(s) 331, 485
Hin1I GRCGYC 1 cut(s) 627
Hin1II CATG 7 cut(s) 96, 339, 367, 757, 1102, 1159, 1301
Hin6I GCGC 2 cut(s) 329, 483
HinP1I GCGC 2 cut(s) 329, 483
HindIII AAGCTT 1 cut(s) 251
HinfI GANTC 5 cut(s) 89, 126, 355, 600, 1102
HpaII CCGG 5 cut(s) 62, 156, 300, 427, 713
HphI GGTGA 1 cut(s) 898
Hpy166II GTNNAC 4 cut(s) 31, 780, 871, 1057
Hpy188I TCNGA 4 cut(s) 78, 243, 284, 1025
Hpy188III TCNNGA 6 cut(s) 62, 364, 550, 713, 1009, 1099
Hpy8I GTNNAC 4 cut(s) 31, 780, 871, 1057
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 4 cut(s) 313, 883, 922, 1168
HpyCH4III ACNGT 8 cut(s) 44, 134, 163, 437, 547, 730, 1033, 1293
HpyCH4IV ACGT 4 cut(s) 57, 640, 867, 1136
HpyCH4V TGCA 6 cut(s) 919, 937, 947, 1070, 1247, 1277
HpyF10VI GCNNNNNNNGC 6 cut(s) 398, 407, 491, 524, 1230, 1247
HpyF3I CTNAG 4 cut(s) 176, 521, 860, 907
HpySE526I ACGT 4 cut(s) 57, 640, 867, 1136
Hsp92I GRCGYC 1 cut(s) 627
Hsp92II CATG 7 cut(s) 96, 339, 367, 757, 1102, 1159, 1301
HspAI GCGC 2 cut(s) 329, 483
Kpn2I TCCGGA 2 cut(s) 61, 712
Ksp22I TGATCA 1 cut(s) 366
Kzo9I GATC 3 cut(s) 292, 366, 1309
LmnI GCTCC 2 cut(s) 668, 1112
Lsp1109I GCAGC 3 cut(s) 397, 1082, 1259
MabI ACCWGGT 1 cut(s) 1161
MaeI CTAG 1 cut(s) 1049
MaeII ACGT 4 cut(s) 57, 640, 867, 1136
MaeIII GTNAC 1 cut(s) 808
MalI GATC 3 cut(s) 294, 368, 1311
MboI GATC 3 cut(s) 292, 366, 1309
MboII GAAGA 4 cut(s) 50, 283, 328, 410
MflI RGATCY 1 cut(s) 292
MluCI AATT 1 cut(s) 1026
MlyI GAGTC 1 cut(s) 1111
MmeI TCCRAC 1 cut(s) 727
MnlI CCTC 8 cut(s) 260, 267, 413, 516, 726, 1031, 1140, 1260
MroI TCCGGA 2 cut(s) 61, 712
MroXI GAANNNNTTC 1 cut(s) 359
MseI TTAA 2 cut(s) 591, 987
MspA1I CMGCKG 3 cut(s) 175, 743, 785
MspI CCGG 5 cut(s) 62, 156, 300, 427, 713
MspR9I CCNGG 6 cut(s) 157, 301, 428, 616, 633, 1163
Mva1269I GAATGC 2 cut(s) 692, 711
MvaI CCWGG 3 cut(s) 616, 633, 1163
MwoI GCNNNNNNNGC 6 cut(s) 398, 407, 491, 524, 1230, 1247
NciI CCSGG 3 cut(s) 157, 301, 428
NdeII GATC 3 cut(s) 292, 366, 1309
NlaIII CATG 7 cut(s) 96, 339, 367, 757, 1102, 1159, 1301
NlaIV GGNNCC 2 cut(s) 171, 1114
NsbI TGCGCA 1 cut(s) 484
NspI RCATGY 2 cut(s) 757, 1159
PagI TCATGA 2 cut(s) 363, 1098
PasI CCCWGGG 2 cut(s) 615, 632
PciI ACATGT 1 cut(s) 1155
PctI GAATGC 2 cut(s) 692, 711
PdmI GAANNNNTTC 1 cut(s) 359
PfeI GAWTC 4 cut(s) 89, 126, 355, 600
PflMI CCANNNNNTGG 2 cut(s) 746, 1088
PfoI TCCNGGA 2 cut(s) 155, 426
PkrI GCNGC 5 cut(s) 174, 412, 1043, 1072, 1249
PleI GAGTC 1 cut(s) 1110
PpsI GAGTC 1 cut(s) 1110
Ppu21I YACGTR 1 cut(s) 641
PscI ACATGT 1 cut(s) 1155
Psp6I CCWGG 3 cut(s) 614, 631, 1161
PspFI CCCAGC 2 cut(s) 145, 532
PspGI CCWGG 3 cut(s) 614, 631, 1161
PspN4I GGNNCC 2 cut(s) 171, 1114
PspPI GGNCC 3 cut(s) 49, 70, 875
PsrI GAACNNNNNNTAC 2 cut(s) 761, 793
PsuI RGATCY 1 cut(s) 292
PvuII CAGCTG 1 cut(s) 785
RsaI GTAC 5 cut(s) 455, 770, 803, 1160, 1295
RsaNI GTAC 5 cut(s) 454, 769, 802, 1159, 1294
SaqAI TTAA 2 cut(s) 591, 987
SatI GCNGC 5 cut(s) 173, 411, 1042, 1071, 1248
Sau3AI GATC 3 cut(s) 292, 366, 1309
Sau96I GGNCC 3 cut(s) 49, 70, 875
SchI GAGTC 1 cut(s) 1111
ScrFI CCNGG 6 cut(s) 157, 301, 428, 616, 633, 1163
SexAI ACCWGGT 1 cut(s) 1161
SfcI CTRYAG 1 cut(s) 726
SinI GGWCC 1 cut(s) 875
SmlI CTYRAG 3 cut(s) 404, 550, 1264
SmoI CTYRAG 3 cut(s) 404, 550, 1264
Sse9I AATT 1 cut(s) 1026
SsiI CCGC 5 cut(s) 104, 173, 470, 743, 1041
SspI AATATT 1 cut(s) 654
SspMI CTAG 1 cut(s) 1049
StyD4I CCNGG 6 cut(s) 155, 299, 426, 614, 631, 1161
StyI CCWWGG 1 cut(s) 1087
TaaI ACNGT 8 cut(s) 44, 134, 163, 437, 547, 730, 1033, 1293
TaiI ACGT 4 cut(s) 60, 643, 870, 1139
TaqI TCGA 1 cut(s) 587
TasI AATT 1 cut(s) 1026
TatI WGTACW 1 cut(s) 768
TauI GCSGC 2 cut(s) 175, 1044
TfiI GAWTC 4 cut(s) 89, 126, 355, 600
Tru1I TTAA 2 cut(s) 591, 987
Tru9I TTAA 2 cut(s) 591, 987
TscAI CASTG 9 cut(s) 16, 49, 139, 220, 379, 442, 525, 582, 735
TseI GCWGC 3 cut(s) 410, 1070, 1247
TspDTI ATGAA 9 cut(s) 17, 81, 245, 352, 458, 1087, 1129, 1179, 1271
TspGWI ACGGA 2 cut(s) 849, 888
TspRI CASTG 9 cut(s) 16, 49, 139, 220, 379, 442, 525, 582, 735
Van91I CCANNNNNTGG 2 cut(s) 746, 1088
VpaK11BI GGWCC 1 cut(s) 875
XceI RCATGY 2 cut(s) 757, 1159
XmiI GTMKAC 1 cut(s) 30
XmnI GAANNNNTTC 1 cut(s) 359
XspI CTAG 1 cut(s) 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.