FvH4_7g24150

Hs1pro-1 N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0013472
Physical Location & Seq
Reverse (-)
18675159 .. 18676595
1437 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g24150.t1

Sequence Viewer

Length: 1437 bp
ATGGTTGATTTGGATTGGAAAGCAAAGATGGTCTCCTCAGATATACCCAGCAAATCACCCAGGCTCTCCAACAAGCTCCACCACCTCTCCATCCCCACCTCCTTCCGCGCCGCCCCTCTCCAGTCCGACATCGCCGCCGCCTCCGACCAAGCCTGCTCCACCTACGAGCACTACCTCCGCCTCCCTGAGCTCCGCCGCCTATGGTCCACCACCGAGTTTCCCGCCTGGAAATCCGAGCCTGTTCTCAAGCCCGCTCTCCAAGGCTTGGAGATCTCCTTCCGCTTCGTCTCCACCGTCGTCTCCGACCCGCGGCCGTACGCTAACCGGAGAGAGTGGCGCCGCCGCCTTGAGTCGTTGATCACTAGTGAGGTTCAGCTCATCGCTTTGTTGATCGAGGACGACGAGGAGGACAGCGAGTCACGCGGCGCGGCGCCGATCGTGGATTTGACGACGTCCGGCGGCGCGTTGGCTCGCGACGGGAGCTTTGCCGAAGTGTGGAGAGGCTCCGGCGAGAGCACGGTGGTGAGCCGCACCAGCGAGTCCAGCCTGCTGCCGCGGCTCGCCGCGTGGCAAAAGTCGGAGTCGGTGGCGCAGAAGATTCTCTACTCGATCGAGTGCGAGATGCGGAGCTGTCCCTACACTCTCGGCCTCGGAGAACCAAACCTCGCCGGAAAACCGAACCTCGAATACGACGCCGTTTGCAAACCGTCGGAGCTTCATTCCTTAAAGAAAAGCCCCTACGATAACTACATCGACAACCACGAGAACCAGGCGTTGTACACCACGCACCAGATCCTCGAGTCGTGGGTGTTCGTCTGCCACGAGCTTCTCAAGCGCGTGAACCGCCGTGTGGAGAGCAACGCCGCCGAAAAAGCCGCGAGCGACTGTTACCTTATCGAGCGCGTCTGGAATCTTCTAGCAGAAATCGAGGACCTCCACCTCTTGATGGACCCGGAGGACTTCCTCCGCCTCAAGAATCAGCTCAAAATCAAAACCGTCGACGATACGGAGTCGTTTTGCTTCCGATCAAAAGGGCTGGTTCAAATCACACAGCTTTGTAAAGACTTGAAGCACAAGGTTCCGAAGGTGCTGGGAGTCGAGGTGGACCCGATGGGCGGGCCGAGAATCCAGGAGGCGGCAATGAGGCTCTACTCGGAGAAAACCGGGTCGGGTAAGATCCATTTGCTTCAGGCGATGCAGAGTATCGAGTCGGCGGTGAAGAGGTTCTACTTTTCGTATAAGCAGGTGTTGGTGGTGTTGATGGGGAGCTTGGAGGCGAAGGGGAACAGGGTCGTGGTGGGACCCGAGTACGGCGACGCGCTGAGTCAGATTTATCTGGAGCCGACGTATTTTCCGAGCTTGGATGCGGCGAAGACGTTTCTTGGGGACGGTTTGAGTCATGAGAACGTGGCGGAGAGACGGACGAGGAGGAGGTAG

Protein Analysis

479

Amino Acids

53.96

Weight (kDa)

6.31

Isoelectric Point (pI)

56.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hs1pro-1_N PF07231 1 - 205 3e-82 Hs1pro-1 N-terminus
Hs1pro-1_C PF07014 208 - 463 1.4e-131 Hs1pro-1 protein C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40000
fragaria_vesca FvH4_7g24150
malus_domestica MD01G1148800.v1.1 MD07G1215700.v1.1
prunus_persica Prupe.2G247600_v2.0.a1
pyrus_communis pycom01g16720 pycom07g19800
rosa_chinensis RchiOBHm_Chr1g0369681
rosa_laevigata RLG00000027112
rosa_multiflora Rmu_sc0001154.1_g000025
rosa_roxburghii Rroxscaffold_2G00131660 Rroxscaffold_4G00287440
rosa_rugosa Rorug01G0351800
rosa_samantha Rh1AG359900 Rh1BG323800 Rh1CG337000 Rh1DG354400
rosa_wichuraiana Rw1G031710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1234
AatII GACGTC 1 cut(s) 455
Acc36I ACCTGC 1 cut(s) 1234
AccB1I GGYRCC 2 cut(s) 336, 430
AccB7I CCANNNNNTGG 1 cut(s) 265
AccBSI CCGCTC 1 cut(s) 254
AccI GTMKAC 1 cut(s) 999
AclWI GGATC 2 cut(s) 787, 1171
AcoI YGGCCR 1 cut(s) 311
AcuI CTGAAG 1 cut(s) 1172
AcyI GRCGYC 4 cut(s) 337, 431, 452, 693
AfaI GTAC 3 cut(s) 317, 779, 1310
AfiI CCNNNNNNNGG 8 cut(s) 265, 309, 495, 850, 946, 1115, 1135, 1310
AgsI TTSAA 2 cut(s) 1043, 1069
AhdI GACNNNNNGTC 1 cut(s) 415
AhlI ACTAGT 1 cut(s) 362
AjnI CCWGG 4 cut(s) 59, 224, 768, 1128
AleI CACNNNNGTG 1 cut(s) 521
Alw21I GWGCWC 3 cut(s) 171, 192, 518
Alw26I GTCTC 4 cut(s) 37, 292, 304, 1411
AlwI GGATC 2 cut(s) 787, 1171
Ama87I CYCGRG 2 cut(s) 797, 1304
AoxI GGCC 3 cut(s) 311, 646, 1118
ApeKI GCWGC 1 cut(s) 550
ArsI GACNNNNNNTTYG 2 cut(s) 467, 499
Asp700I GAANNNNTTC 1 cut(s) 1223
AspLEI GCGC 9 cut(s) 110, 339, 428, 433, 464, 592, 837, 903, 1321
AspS9I GGNCC 6 cut(s) 204, 931, 949, 1105, 1118, 1301
AsuC2I CCSGG 2 cut(s) 953, 1165
AsuHPI GGTGA 3 cut(s) 48, 535, 1228
AvaI CYCGRG 2 cut(s) 797, 1304
AvaII GGWCC 5 cut(s) 204, 931, 949, 1105, 1301
BanI GGYRCC 2 cut(s) 336, 430
BanII GRGCYC 1 cut(s) 192
BarI GAAGNNNNNNTAC 4 cut(s) 587, 619, 1211, 1243
BauI CACGAG 2 cut(s) 761, 821
BbsI GAAGAC 1 cut(s) 1379
Bbv12I GWGCWC 3 cut(s) 171, 192, 518
BbvI GCAGC 1 cut(s) 537
BccI CCATC 5 cut(s) 22, 98, 940, 1105, 1255
BceAI ACGGC 4 cut(s) 298, 680, 831, 1327
BciT130I CCWGG 4 cut(s) 61, 226, 770, 1130
BclI TGATCA 1 cut(s) 357
BcnI CCSGG 2 cut(s) 953, 1165
BcoDI GTCTC 4 cut(s) 37, 292, 304, 1411
BcuI ACTAGT 1 cut(s) 362
BfaI CTAG 2 cut(s) 363, 917
BfoI RGCGCY 2 cut(s) 340, 434
BfuAI ACCTGC 1 cut(s) 1234
BglII AGATCT 1 cut(s) 270
Bme1390I CCNGG 6 cut(s) 61, 226, 770, 953, 1130, 1165
Bme18I GGWCC 5 cut(s) 204, 931, 949, 1105, 1301
BmeRI GACNNNNNGTC 1 cut(s) 415
BmeT110I CYCGRG 2 cut(s) 797, 1304
BmgT120I GGNCC 6 cut(s) 204, 931, 949, 1105, 1118, 1301
BmiI GGNNCC 9 cut(s) 338, 432, 505, 951, 1080, 1107, 1302, 1303, 1341
BmrFI CCNGG 6 cut(s) 61, 226, 770, 953, 1130, 1165
BmsI GCATC 3 cut(s) 612, 1185, 1354
BpiI GAAGAC 1 cut(s) 1379
BpmI CTGGAG 2 cut(s) 104, 1358
Bpu10I CCTNAGC 1 cut(s) 186
BpuEI CTTGAG 4 cut(s) 230, 368, 815, 956
BpuMI CCSGG 2 cut(s) 953, 1165
BsaHI GRCGYC 4 cut(s) 337, 431, 452, 693
BsaI GGTCTC 1 cut(s) 37
BsaJI CCNNGG 5 cut(s) 59, 259, 308, 554, 649
BsaWI WCCGGW 1 cut(s) 324
BsaXI ACNNNNNCTCC 6 cut(s) 71, 101, 1086, 1116, 1331, 1361
Bsc4I CCNNNNNNNGG 8 cut(s) 265, 309, 495, 850, 946, 1115, 1135, 1310
Bse1I ACTGG 1 cut(s) 121
Bse3DI GCAATG 1 cut(s) 1146
BseBI CCWGG 4 cut(s) 61, 226, 770, 1130
BseDI CCNNGG 5 cut(s) 59, 259, 308, 554, 649
BseGI GGATG 2 cut(s) 90, 1369
BseLI CCNNNNNNNGG 8 cut(s) 265, 309, 495, 850, 946, 1115, 1135, 1310
BseMI GCAATG 1 cut(s) 1146
BseMII CTCAG 3 cut(s) 51, 177, 1313
BseNI ACTGG 1 cut(s) 121
BseRI GAGGAG 2 cut(s) 25, 419
BseX3I CGGCCG 1 cut(s) 311
BseXI GCAGC 1 cut(s) 537
BseYI CCCAGC 2 cut(s) 47, 1090
Bsh1285I CGRYCG 3 cut(s) 314, 438, 612
BshFI GGCC 3 cut(s) 313, 648, 1120
BshNI GGYRCC 2 cut(s) 336, 430
BsiEI CGRYCG 3 cut(s) 314, 438, 612
BsiHKAI GWGCWC 3 cut(s) 171, 192, 518
BsiHKCI CYCGRG 2 cut(s) 797, 1304
BsiSI CCGG 6 cut(s) 325, 456, 507, 669, 953, 1164
BsiWI CGTACG 1 cut(s) 315
BslFI GGGAC 3 cut(s) 618, 1314, 1400
BslI CCNNNNNNNGG 8 cut(s) 265, 309, 495, 850, 946, 1115, 1135, 1310
BsmAI GTCTC 4 cut(s) 37, 292, 304, 1411
BsmBI CGTCTC 3 cut(s) 292, 304, 1411
BsmFI GGGAC 3 cut(s) 618, 1314, 1400
BsnI GGCC 3 cut(s) 313, 648, 1120
Bso31I GGTCTC 1 cut(s) 37
BsoBI CYCGRG 2 cut(s) 797, 1304
Bsp1286I GDGCHC 3 cut(s) 171, 192, 518
Bsp1407I TGTACA 1 cut(s) 777
Bsp143I GATC 8 cut(s) 270, 357, 390, 435, 609, 792, 1025, 1176
Bsp68I TCGCGA 1 cut(s) 474
BspANI GGCC 3 cut(s) 313, 648, 1120
BspCNI CTCAG 3 cut(s) 50, 178, 1314
BspHI TCATGA 1 cut(s) 1399
BspLI GGNNCC 9 cut(s) 338, 432, 505, 951, 1080, 1107, 1302, 1303, 1341
BspMI ACCTGC 1 cut(s) 1234
BspPI GGATC 2 cut(s) 787, 1171
BspT107I GGYRCC 2 cut(s) 336, 430
BspTNI GGTCTC 1 cut(s) 37
BsrBI CCGCTC 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 1146
BsrGI TGTACA 1 cut(s) 777
BsrI ACTGG 1 cut(s) 121
BssECI CCNNGG 5 cut(s) 59, 259, 308, 554, 649
BssMI GATC 8 cut(s) 270, 357, 390, 435, 609, 792, 1025, 1176
BssNI GRCGYC 4 cut(s) 337, 431, 452, 693
BssSI CACGAG 2 cut(s) 761, 821
BssT1I CCWWGG 1 cut(s) 259
Bst2BI CACGAG 2 cut(s) 761, 821
Bst2UI CCWGG 4 cut(s) 61, 226, 770, 1130
Bst4CI ACNGT 6 cut(s) 295, 520, 708, 887, 997, 1391
Bst6I CTCTTC 1 cut(s) 1214
BstACI GRCGYC 4 cut(s) 337, 431, 452, 693
BstAUI TGTACA 1 cut(s) 777
BstC8I GCNNGC 7 cut(s) 154, 252, 472, 548, 561, 880, 1118
BstDEI CTNAG 3 cut(s) 37, 186, 1322
BstDSI CCRYGG 2 cut(s) 308, 554
BstF5I GGATG 2 cut(s) 90, 1369
BstH2I RGCGCY 2 cut(s) 340, 434
BstHHI GCGC 9 cut(s) 110, 339, 428, 433, 464, 592, 837, 903, 1321
BstKTI GATC 8 cut(s) 273, 360, 393, 438, 612, 795, 1028, 1179
BstMAI GTCTC 4 cut(s) 37, 292, 304, 1411
BstMBI GATC 8 cut(s) 270, 357, 390, 435, 609, 792, 1025, 1176
BstMCI CGRYCG 3 cut(s) 314, 438, 612
BstMWI GCNNNNNNNGC 8 cut(s) 420, 480, 534, 543, 556, 832, 843, 872
BstNI CCWGG 4 cut(s) 61, 226, 770, 1130
BstSCI CCNGG 6 cut(s) 59, 224, 768, 951, 1128, 1163
BstV1I GCAGC 1 cut(s) 537
BstV2I GAAGAC 1 cut(s) 1379
BstX2I RGATCY 3 cut(s) 270, 792, 1176
BstYI RGATCY 3 cut(s) 270, 792, 1176
BstZI CGGCCG 1 cut(s) 311
BsuRI GGCC 3 cut(s) 313, 648, 1120
BtgI CCRYGG 2 cut(s) 308, 554
BtgZI GCGATG 3 cut(s) 115, 364, 1208
BtsCI GGATG 2 cut(s) 90, 1369
BtuMI TCGCGA 1 cut(s) 474
BveI ACCTGC 1 cut(s) 1234
Cac8I GCNNGC 7 cut(s) 154, 252, 472, 548, 561, 880, 1118
CciI TCATGA 1 cut(s) 1399
CfoI GCGC 9 cut(s) 110, 339, 428, 433, 464, 592, 837, 903, 1321
Cfr13I GGNCC 6 cut(s) 204, 931, 949, 1105, 1118, 1301
Cfr42I CCGCGG 2 cut(s) 311, 557
CseI GACGC 3 cut(s) 701, 892, 1325
Csp6I GTAC 3 cut(s) 316, 778, 1309
CviAII CATG 1 cut(s) 1400
CviQI GTAC 3 cut(s) 316, 778, 1309
DdeI CTNAG 3 cut(s) 37, 186, 1322
DinI GGCGCC 2 cut(s) 338, 432
DpnI GATC 8 cut(s) 272, 359, 392, 437, 611, 794, 1027, 1178
DpnII GATC 8 cut(s) 270, 357, 390, 435, 609, 792, 1025, 1176
DriI GACNNNNNGTC 1 cut(s) 415
EaeI YGGCCR 1 cut(s) 311
EagI CGGCCG 1 cut(s) 311
Eam1104I CTCTTC 1 cut(s) 1214
Eam1105I GACNNNNNGTC 1 cut(s) 415
EarI CTCTTC 1 cut(s) 1214
EciI GGCGGA 4 cut(s) 167, 182, 956, 1427
Ecl136II GAGCTC 1 cut(s) 190
EclXI CGGCCG 1 cut(s) 311
Eco130I CCWWGG 1 cut(s) 259
Eco24I GRGCYC 1 cut(s) 192
Eco31I GGTCTC 1 cut(s) 37
Eco47I GGWCC 5 cut(s) 204, 931, 949, 1105, 1301
Eco52I CGGCCG 1 cut(s) 311
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 1172
Eco88I CYCGRG 2 cut(s) 797, 1304
EcoICRI GAGCTC 1 cut(s) 190
EcoO109I RGGNCCY 2 cut(s) 931, 1301
EcoRII CCWGG 4 cut(s) 59, 224, 768, 1128
EcoT14I CCWWGG 1 cut(s) 259
EcoT38I GRGCYC 1 cut(s) 192
EgeI GGCGCC 2 cut(s) 338, 432
EheI GGCGCC 2 cut(s) 338, 432
ErhI CCWWGG 1 cut(s) 259
Esp3I CGTCTC 3 cut(s) 292, 304, 1411
FaeI CATG 1 cut(s) 1403
FaiI YATR 4 cut(s) 44, 202, 1239, 1401
FaqI GGGAC 3 cut(s) 618, 1314, 1400
FatI CATG 1 cut(s) 1399
FauI CCCGC 4 cut(s) 229, 259, 315, 1109
FbaI TGATCA 1 cut(s) 357
FblI GTMKAC 1 cut(s) 999
FokI GGATG 2 cut(s) 77, 1376
FriOI GRGCYC 1 cut(s) 192
FspBI CTAG 2 cut(s) 363, 917
GlaI GCGC 9 cut(s) 109, 338, 427, 432, 463, 591, 836, 902, 1320
GsaI CCCAGC 2 cut(s) 51, 1094
GsuI CTGGAG 2 cut(s) 104, 1358
HaeII RGCGCY 2 cut(s) 340, 434
HaeIII GGCC 3 cut(s) 313, 648, 1120
HapII CCGG 6 cut(s) 325, 456, 507, 669, 953, 1164
HgaI GACGC 3 cut(s) 701, 892, 1325
HhaI GCGC 9 cut(s) 110, 339, 428, 433, 464, 592, 837, 903, 1321
Hin1I GRCGYC 4 cut(s) 337, 431, 452, 693
Hin1II CATG 1 cut(s) 1403
Hin6I GCGC 9 cut(s) 108, 337, 426, 431, 462, 590, 835, 901, 1319
HinP1I GCGC 9 cut(s) 108, 337, 426, 431, 462, 590, 835, 901, 1319
HincII GTYRAC 1 cut(s) 1000
HindII GTYRAC 1 cut(s) 1000
HpaII CCGG 6 cut(s) 325, 456, 507, 669, 953, 1164
HphI GGTGA 3 cut(s) 48, 535, 1228
Hpy166II GTNNAC 5 cut(s) 207, 780, 841, 1000, 1105
Hpy188III TCNNGA 6 cut(s) 473, 907, 943, 973, 1337, 1400
Hpy8I GTNNAC 5 cut(s) 207, 780, 841, 1000, 1105
HpyAV CCTTC 4 cut(s) 112, 286, 1078, 1273
HpyCH4III ACNGT 6 cut(s) 295, 520, 708, 887, 997, 1391
HpyCH4IV ACGT 4 cut(s) 452, 1346, 1376, 1407
HpyCH4V TGCA 2 cut(s) 702, 1198
HpyF10VI GCNNNNNNNGC 8 cut(s) 420, 480, 534, 543, 556, 832, 843, 872
HpyF3I CTNAG 3 cut(s) 37, 186, 1322
HpySE526I ACGT 4 cut(s) 452, 1346, 1376, 1407
Hsp92I GRCGYC 4 cut(s) 337, 431, 452, 693
Hsp92II CATG 1 cut(s) 1403
HspAI GCGC 9 cut(s) 108, 337, 426, 431, 462, 590, 835, 901, 1319
KasI GGCGCC 2 cut(s) 336, 430
KflI GGGWCCC 1 cut(s) 1301
Ksp22I TGATCA 1 cut(s) 357
KspI CCGCGG 2 cut(s) 311, 557
Kzo9I GATC 8 cut(s) 270, 357, 390, 435, 609, 792, 1025, 1176
LmnI GCTCC 9 cut(s) 81, 161, 195, 480, 509, 627, 712, 1266, 1339
Lsp1109I GCAGC 1 cut(s) 537
LweI GCATC 3 cut(s) 612, 1185, 1354
MaeI CTAG 2 cut(s) 363, 917
MaeII ACGT 4 cut(s) 452, 1346, 1376, 1407
MaeIII GTNAC 2 cut(s) 417, 887
MalI GATC 8 cut(s) 272, 359, 392, 437, 611, 794, 1027, 1178
MbiI CCGCTC 1 cut(s) 254
MboI GATC 8 cut(s) 270, 357, 390, 435, 609, 792, 1025, 1176
MboII GAAGA 4 cut(s) 607, 905, 1231, 1384
MflI RGATCY 3 cut(s) 270, 792, 1176
MhlI GDGCHC 3 cut(s) 171, 192, 518
Mly113I GGCGCC 2 cut(s) 337, 431
MmeI TCCRAC 6 cut(s) 93, 150, 168, 327, 558, 690
MroXI GAANNNNTTC 1 cut(s) 1223
MseI TTAA 1 cut(s) 725
MslI CAYNNNNRTG 1 cut(s) 521
MspA1I CMGCKG 2 cut(s) 310, 556
MspI CCGG 6 cut(s) 325, 456, 507, 669, 953, 1164
MspR9I CCNGG 6 cut(s) 61, 226, 770, 953, 1130, 1165
MteI GCGCNGCGC 1 cut(s) 429
MvaI CCWGG 4 cut(s) 61, 226, 770, 1130
MwoI GCNNNNNNNGC 8 cut(s) 420, 480, 534, 543, 556, 832, 843, 872
NarI GGCGCC 2 cut(s) 337, 431
NciI CCSGG 2 cut(s) 953, 1165
NdeII GATC 8 cut(s) 270, 357, 390, 435, 609, 792, 1025, 1176
NlaIII CATG 1 cut(s) 1403
NlaIV GGNNCC 9 cut(s) 338, 432, 505, 951, 1080, 1107, 1302, 1303, 1341
NmeAIII GCCGAG 2 cut(s) 624, 1146
NmuCI GTSAC 1 cut(s) 417
NruI TCGCGA 1 cut(s) 474
OliI CACNNNNGTG 1 cut(s) 521
PaeR7I CTCGAG 1 cut(s) 797
PagI TCATGA 1 cut(s) 1399
PaqCI CACCTGC 1 cut(s) 1234
PcsI WCGNNNNNNNCGW 5 cut(s) 291, 399, 759, 819, 1352
PdmI GAANNNNTTC 1 cut(s) 1223
PfeI GAWTC 4 cut(s) 598, 910, 976, 1125
Pfl23II CGTACG 1 cut(s) 315
PflFI GACNNNGTC 1 cut(s) 451
PflMI CCANNNNNTGG 1 cut(s) 265
PfoI TCCNGGA 1 cut(s) 1128
Ple19I CGATCG 2 cut(s) 438, 612
PluTI GGCGCC 2 cut(s) 340, 434
PpuMI RGGWCCY 2 cut(s) 931, 1301
Psp124BI GAGCTC 1 cut(s) 192
Psp5II RGGWCCY 2 cut(s) 931, 1301
Psp6I CCWGG 4 cut(s) 59, 224, 768, 1128
PspFI CCCAGC 2 cut(s) 47, 1090
PspGI CCWGG 4 cut(s) 59, 224, 768, 1128
PspLI CGTACG 1 cut(s) 315
PspN4I GGNNCC 9 cut(s) 338, 432, 505, 951, 1080, 1107, 1302, 1303, 1341
PspPI GGNCC 6 cut(s) 204, 931, 949, 1105, 1118, 1301
PspPPI RGGWCCY 2 cut(s) 931, 1301
PspXI VCTCGAGB 1 cut(s) 797
PsrI GAACNNNNNNTAC 2 cut(s) 671, 703
PsuI RGATCY 3 cut(s) 270, 792, 1176
PsyI GACNNNGTC 1 cut(s) 451
PvuI CGATCG 2 cut(s) 438, 612
RruI TCGCGA 1 cut(s) 474
RsaI GTAC 3 cut(s) 317, 779, 1310
RsaNI GTAC 3 cut(s) 316, 778, 1309
RseI CAYNNNNRTG 1 cut(s) 521
SacI GAGCTC 1 cut(s) 192
SacII CCGCGG 2 cut(s) 311, 557
SalI GTCGAC 1 cut(s) 998
SaqAI TTAA 1 cut(s) 725
Sau3AI GATC 8 cut(s) 270, 357, 390, 435, 609, 792, 1025, 1176
Sau96I GGNCC 6 cut(s) 204, 931, 949, 1105, 1118, 1301
ScrFI CCNGG 6 cut(s) 61, 226, 770, 953, 1130, 1165
SduI GDGCHC 3 cut(s) 171, 192, 518
SfaNI GCATC 3 cut(s) 612, 1185, 1354
SfoI GGCGCC 2 cut(s) 338, 432
Sfr274I CTCGAG 1 cut(s) 797
Sfr303I CCGCGG 2 cut(s) 311, 557
SgrBI CCGCGG 2 cut(s) 311, 557
SgrDI CGTCGACG 1 cut(s) 998
SinI GGWCC 5 cut(s) 204, 931, 949, 1105, 1301
SlaI CTCGAG 1 cut(s) 797
SmiMI CAYNNNNRTG 1 cut(s) 521
SmlI CTYRAG 5 cut(s) 245, 347, 797, 830, 971
SmoI CTYRAG 5 cut(s) 245, 347, 797, 830, 971
SpeI ACTAGT 1 cut(s) 362
SspDI GGCGCC 2 cut(s) 336, 430
SspMI CTAG 2 cut(s) 363, 917
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 6 cut(s) 59, 224, 768, 951, 1128, 1163
StyI CCWWGG 1 cut(s) 259
TaaI ACNGT 6 cut(s) 295, 520, 708, 887, 997, 1391
TaiI ACGT 4 cut(s) 455, 1349, 1379, 1410
TatI WGTACW 1 cut(s) 777
TfiI GAWTC 4 cut(s) 598, 910, 976, 1125
Tru1I TTAA 1 cut(s) 725
Tru9I TTAA 1 cut(s) 725
TseFI GTSAC 1 cut(s) 417
TseI GCWGC 1 cut(s) 550
Tsp45I GTSAC 1 cut(s) 417
TspDTI ATGAA 1 cut(s) 707
TspGWI ACGGA 2 cut(s) 1022, 1435
Tth111I GACNNNGTC 1 cut(s) 451
Van91I CCANNNNNTGG 1 cut(s) 265
VpaK11BI GGWCC 5 cut(s) 204, 931, 949, 1105, 1301
XhoI CTCGAG 1 cut(s) 797
XmiI GTMKAC 1 cut(s) 999
XmnI GAANNNNTTC 1 cut(s) 1223
XspI CTAG 2 cut(s) 363, 917
ZraI GACGTC 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.