RLG00000027112

Hs1pro-1 N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0013472
Physical Location & Seq
Forward (+)
8519070 .. 8521827
2758 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027112

Sequence Viewer

Length: 1440 bp
ATGGTTGATTTGGATTGGAAAGCAAAAATGGTCTCCTCAGATATACCCAGCAAGTCCCCCAGGCTCTCCAACAAGCTCCACCACCTCTCCATCCCCGGCTCCTTCCGCGCCGCCCAAATCCAGTCCGACATTTCCCCCGCCTCCGAGCAGGCCTGCTCCACCTACGAGCACTACCTCCGCCTCCCCGAGCTCCGCAAGCTCTGGTGCTCCACCGACTTCCCCGCCTGGAAAAGCGAGTCCATTCTCAAGCCCGCTCTCCAAGCTCTCGAGATCTCTTTCCGCTTCGTCTCCACCGTCCTCTCCGACTCCAGGCCCTACGCCAACCGGAGAGAGTGGCGCCGCCGCCTCGAGTCGCTTATTACTGGTCAGGTTGAGCTCATCGCTTTGTTGATCGAGGACGATGAGGAGGACAGCGAGACGCGCGGAGCGGCGCCGATCGTTGATCTGAGCAATTCCGGCGGCGCGTTGGCTCGCAACGGGAGCTTGGCCGAGGTGTGGAGGGGCTCCGGCGAGAGCACCGTGGTGAGCCGCATCAGCGAGGCCAGCCTGCTGCCTCGGCTCGCCGCGTGGCAGAAGTCGGAGTCGATGGCGCAGAAGATCCTGTACTCGATCGAGTGCGAGATGCGGAGCTGTCCGTACACTCTAGGACTCGGCGAGCCGAACCTCGCCGGAAAACCGAACCTGGAATACGACGCCGTTTGCAAGCCGTCGGAGCTGCATTTGCTAAAGAGGAGCCCGTACGATAGCCACATCCACAACCACGAGAACCAGGCTGTGTACACCACGCACCAGGTACTCGAGTCGTGGGTGTTCGCCTGCCACGAGCTCCTCAAGCGCGTGAACCGCCGCGTCGAGTCCAGCGACTTCGAAAAGGCCGCCACCGACTGTTACTTGATCGAGCGCGTCTGGAAGCTTCTGGCGGAAATCGAGGACCTCCACCTGTTAATGGACCCGGAGGACTTTCTCCGGCTCAAGAACCAGTTGAAGATCAAAACGGTGGACGATACGGAGTCGTTTTGCTTCAGGTCGAAGGGACTGGTGGAGATCACAAGGCTGTGTAAGGACTTGAAGCACAAGGTTCCGAACGTGCTGGGAGTGGAGGTGGACCCGATGGGTGGGCCGAGGATCCAGGAGGCGGCGATGAGGCTCTACTCGGAGAAAACCGGGTCGGAGAAGATCCATCTCCTTCAGGCAATGCAGACTATAGAATCGGCGGTGAAACGTTTCTTCTTTGCGTACAAGCAGGTGCTGGTGGTGTTGATGGGGAGCTTGGAGGCGAAAGGGAACCGGGTCGTGGTGAGCCCGGAGTCCGGCGACGCGCTGAGTCAGATATTCCTGGAGCCGACGTATTTTCCGAGCTTGGACGCCGCGAAGACGTTTCTGGGGGACGGTTTGAGTCACGAAAACGTTTCGGAGAGACGGACGCGGAGGACTGGGTAG

Protein Analysis

480

Amino Acids

54.08

Weight (kDa)

6.27

Isoelectric Point (pI)

58.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hs1pro-1_N PF07231 1 - 205 2e-82 Hs1pro-1 N-terminus
Hs1pro-1_C PF07014 208 - 463 4.2e-134 Hs1pro-1 protein C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40000
fragaria_vesca FvH4_7g24150
malus_domestica MD01G1148800.v1.1 MD07G1215700.v1.1
prunus_persica Prupe.2G247600_v2.0.a1
pyrus_communis pycom01g16720 pycom07g19800
rosa_chinensis RchiOBHm_Chr1g0369681
rosa_laevigata RLG00000027112
rosa_multiflora Rmu_sc0001154.1_g000025
rosa_roxburghii Rroxscaffold_2G00131660 Rroxscaffold_4G00287440
rosa_rugosa Rorug01G0351800
rosa_samantha Rh1AG359900 Rh1BG323800 Rh1CG337000 Rh1DG354400
rosa_wichuraiana Rw1G031710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1234
Acc36I ACCTGC 1 cut(s) 1234
AccB1I GGYRCC 2 cut(s) 336, 430
AccBSI CCGCTC 2 cut(s) 254, 428
AclI AACGTT 2 cut(s) 1222, 1407
AclWI GGATC 4 cut(s) 592, 1120, 1133, 1171
AcoI YGGCCR 1 cut(s) 486
AcuI CTGAAG 2 cut(s) 1006, 1172
AcyI GRCGYC 4 cut(s) 337, 431, 693, 1365
AfaI GTAC 6 cut(s) 605, 638, 740, 779, 795, 1238
AfiI CCNNNNNNNGG 7 cut(s) 309, 495, 946, 1115, 1135, 1294, 1310
AgsI TTSAA 2 cut(s) 985, 1069
AjnI CCWGG 8 cut(s) 59, 224, 308, 681, 768, 789, 1128, 1335
AleI CACNNNNGTG 1 cut(s) 521
Alw21I GWGCWC 6 cut(s) 171, 192, 209, 378, 518, 828
Alw26I GTCTC 4 cut(s) 37, 292, 410, 1411
AlwI GGATC 4 cut(s) 592, 1120, 1133, 1171
AlwNI CAGNNNCTG 1 cut(s) 1249
Ama87I CYCGRG 4 cut(s) 185, 266, 347, 797
AoxI GGCC 6 cut(s) 150, 311, 486, 540, 873, 1118
ApeKI GCWGC 2 cut(s) 550, 715
Asp700I GAANNNNTTC 1 cut(s) 1223
AspLEI GCGC 9 cut(s) 110, 339, 423, 433, 464, 592, 837, 903, 1321
AspS9I GGNCC 5 cut(s) 312, 931, 949, 1105, 1118
AsuC2I CCSGG 5 cut(s) 96, 953, 1165, 1289, 1304
AsuHPI GGTGA 3 cut(s) 535, 1228, 1309
AsuII TTCGAA 1 cut(s) 867
AvaI CYCGRG 4 cut(s) 185, 266, 347, 797
AvaII GGWCC 3 cut(s) 931, 949, 1105
BaeI ACNNNNGTAYC 2 cut(s) 785, 818
BamHI GGATCC 1 cut(s) 1125
BanI GGYRCC 2 cut(s) 336, 430
BanII GRGCYC 6 cut(s) 192, 378, 506, 737, 828, 1304
BarI GAAGNNNNNNTAC 2 cut(s) 587, 619
BauI CACGAG 2 cut(s) 761, 821
BbsI GAAGAC 1 cut(s) 1379
Bbv12I GWGCWC 6 cut(s) 171, 192, 209, 378, 518, 828
BbvI GCAGC 2 cut(s) 537, 702
BccI CCATC 5 cut(s) 98, 580, 1105, 1188, 1255
BceAI ACGGC 2 cut(s) 680, 691
BciT130I CCWGG 8 cut(s) 61, 226, 310, 683, 770, 791, 1130, 1337
BcnI CCSGG 5 cut(s) 96, 953, 1165, 1289, 1304
BcoDI GTCTC 4 cut(s) 37, 292, 410, 1411
BfaI CTAG 1 cut(s) 644
BfmI CTRYAG 1 cut(s) 1203
BfoI RGCGCY 2 cut(s) 340, 434
BfuAI ACCTGC 1 cut(s) 1234
BglII AGATCT 1 cut(s) 270
Bme18I GGWCC 3 cut(s) 931, 949, 1105
BmeT110I CYCGRG 4 cut(s) 185, 266, 347, 797
BmgT120I GGNCC 5 cut(s) 312, 931, 949, 1105, 1118
BmsI GCATC 2 cut(s) 540, 612
BpiI GAAGAC 1 cut(s) 1379
BpmI CTGGAG 2 cut(s) 292, 1358
Bpu14I TTCGAA 1 cut(s) 867
BpuEI CTTGAG 3 cut(s) 230, 815, 956
BpuMI CCSGG 5 cut(s) 96, 953, 1165, 1289, 1304
BsaHI GRCGYC 4 cut(s) 337, 431, 693, 1365
BsaI GGTCTC 1 cut(s) 37
BsaJI CCNNGG 6 cut(s) 59, 94, 489, 519, 554, 1121
BsaWI WCCGGW 1 cut(s) 324
BsaXI ACNNNNNCTCC 6 cut(s) 71, 101, 1086, 1116, 1331, 1361
Bsc4I CCNNNNNNNGG 7 cut(s) 309, 495, 946, 1115, 1135, 1294, 1310
Bse1I ACTGG 5 cut(s) 121, 367, 979, 1041, 1438
Bse3DI GCAATG 1 cut(s) 1200
BseBI CCWGG 8 cut(s) 61, 226, 310, 683, 770, 791, 1130, 1337
BseDI CCNNGG 6 cut(s) 59, 94, 489, 519, 554, 1121
BseGI GGATG 2 cut(s) 90, 750
BseLI CCNNNNNNNGG 7 cut(s) 309, 495, 946, 1115, 1135, 1294, 1310
BseMI GCAATG 1 cut(s) 1200
BseMII CTCAG 3 cut(s) 51, 437, 1313
BseNI ACTGG 5 cut(s) 121, 367, 979, 1041, 1438
BseRI GAGGAG 4 cut(s) 25, 419, 745, 818
BseXI GCAGC 2 cut(s) 537, 702
BseYI CCCAGC 2 cut(s) 47, 1090
Bsh1285I CGRYCG 2 cut(s) 438, 612
BshFI GGCC 6 cut(s) 152, 313, 488, 542, 875, 1120
BshNI GGYRCC 2 cut(s) 336, 430
BsiEI CGRYCG 2 cut(s) 438, 612
BsiHKAI GWGCWC 6 cut(s) 171, 192, 209, 378, 518, 828
BsiHKCI CYCGRG 4 cut(s) 185, 266, 347, 797
BsiWI CGTACG 1 cut(s) 738
BslFI GGGAC 3 cut(s) 40, 1047, 1400
BslI CCNNNNNNNGG 7 cut(s) 309, 495, 946, 1115, 1135, 1294, 1310
BsmAI GTCTC 4 cut(s) 37, 292, 410, 1411
BsmBI CGTCTC 3 cut(s) 292, 410, 1411
BsmFI GGGAC 3 cut(s) 40, 1047, 1400
BsnI GGCC 6 cut(s) 152, 313, 488, 542, 875, 1120
Bso31I GGTCTC 1 cut(s) 37
BsoBI CYCGRG 4 cut(s) 185, 266, 347, 797
Bsp119I TTCGAA 1 cut(s) 867
Bsp1286I GDGCHC 9 cut(s) 171, 192, 209, 378, 506, 518, 737, 828, 1304
Bsp1407I TGTACA 1 cut(s) 777
BspANI GGCC 6 cut(s) 152, 313, 488, 542, 875, 1120
BspCNI CTCAG 3 cut(s) 50, 438, 1314
BspMI ACCTGC 1 cut(s) 1234
BspPI GGATC 4 cut(s) 592, 1120, 1133, 1171
BspT104I TTCGAA 1 cut(s) 867
BspT107I GGYRCC 2 cut(s) 336, 430
BspTNI GGTCTC 1 cut(s) 37
BsrBI CCGCTC 2 cut(s) 254, 428
BsrDI GCAATG 1 cut(s) 1200
BsrGI TGTACA 1 cut(s) 777
BsrI ACTGG 5 cut(s) 121, 367, 979, 1041, 1438
BssECI CCNNGG 6 cut(s) 59, 94, 489, 519, 554, 1121
BssNI GRCGYC 4 cut(s) 337, 431, 693, 1365
BssSI CACGAG 2 cut(s) 761, 821
Bst2BI CACGAG 2 cut(s) 761, 821
Bst2UI CCWGG 8 cut(s) 61, 226, 310, 683, 770, 791, 1130, 1337
Bst4CI ACNGT 5 cut(s) 295, 520, 887, 997, 1391
BstACI GRCGYC 4 cut(s) 337, 431, 693, 1365
BstAUI TGTACA 1 cut(s) 777
BstBI TTCGAA 1 cut(s) 867
BstDEI CTNAG 3 cut(s) 37, 446, 1322
BstDSI CCRYGG 1 cut(s) 519
BstF5I GGATG 2 cut(s) 90, 750
BstH2I RGCGCY 2 cut(s) 340, 434
BstHHI GCGC 9 cut(s) 110, 339, 423, 433, 464, 592, 837, 903, 1321
BstMAI GTCTC 4 cut(s) 37, 292, 410, 1411
BstMCI CGRYCG 2 cut(s) 438, 612
BstNI CCWGG 8 cut(s) 61, 226, 310, 683, 770, 791, 1130, 1337
BstSFI CTRYAG 1 cut(s) 1203
BstV1I GCAGC 2 cut(s) 537, 702
BstV2I GAAGAC 1 cut(s) 1379
BstX2I RGATCY 4 cut(s) 270, 597, 1125, 1176
BstYI RGATCY 4 cut(s) 270, 597, 1125, 1176
BsuRI GGCC 6 cut(s) 152, 313, 488, 542, 875, 1120
BtgI CCRYGG 1 cut(s) 519
BtgZI GCGATG 2 cut(s) 364, 1154
BtsCI GGATG 2 cut(s) 90, 750
BveI ACCTGC 1 cut(s) 1234
CaiI CAGNNNCTG 1 cut(s) 1249
CfoI GCGC 9 cut(s) 110, 339, 423, 433, 464, 592, 837, 903, 1321
Cfr13I GGNCC 5 cut(s) 312, 931, 949, 1105, 1118
CseI GACGC 7 cut(s) 427, 701, 838, 892, 1325, 1373, 1432
CsiI ACCWGGT 1 cut(s) 789
Csp6I GTAC 6 cut(s) 604, 637, 739, 778, 794, 1237
CviQI GTAC 6 cut(s) 604, 637, 739, 778, 794, 1237
DdeI CTNAG 3 cut(s) 37, 446, 1322
DinI GGCGCC 2 cut(s) 338, 432
EaeI YGGCCR 1 cut(s) 486
EciI GGCGGA 2 cut(s) 167, 935
Ecl136II GAGCTC 3 cut(s) 190, 376, 826
Eco147I AGGCCT 1 cut(s) 152
Eco24I GRGCYC 6 cut(s) 192, 378, 506, 737, 828, 1304
Eco31I GGTCTC 1 cut(s) 37
Eco47I GGWCC 3 cut(s) 931, 949, 1105
Eco53kI GAGCTC 3 cut(s) 190, 376, 826
Eco57I CTGAAG 2 cut(s) 1006, 1172
Eco88I CYCGRG 4 cut(s) 185, 266, 347, 797
EcoICRI GAGCTC 3 cut(s) 190, 376, 826
EcoO109I RGGNCCY 2 cut(s) 312, 931
EcoRII CCWGG 8 cut(s) 59, 224, 308, 681, 768, 789, 1128, 1335
EcoT38I GRGCYC 6 cut(s) 192, 378, 506, 737, 828, 1304
EgeI GGCGCC 2 cut(s) 338, 432
EheI GGCGCC 2 cut(s) 338, 432
Esp3I CGTCTC 3 cut(s) 292, 410, 1411
FaiI YATR 2 cut(s) 44, 1205
FaqI GGGAC 3 cut(s) 40, 1047, 1400
FauI CCCGC 3 cut(s) 145, 229, 259
FokI GGATG 2 cut(s) 77, 737
FriOI GRGCYC 6 cut(s) 192, 378, 506, 737, 828, 1304
FspBI CTAG 1 cut(s) 644
GlaI GCGC 9 cut(s) 109, 338, 422, 432, 463, 591, 836, 902, 1320
GsaI CCCAGC 2 cut(s) 51, 1094
GsuI CTGGAG 2 cut(s) 292, 1358
HaeII RGCGCY 2 cut(s) 340, 434
HaeIII GGCC 6 cut(s) 152, 313, 488, 542, 875, 1120
HgaI GACGC 7 cut(s) 427, 701, 838, 892, 1325, 1373, 1432
HhaI GCGC 9 cut(s) 110, 339, 423, 433, 464, 592, 837, 903, 1321
Hin1I GRCGYC 4 cut(s) 337, 431, 693, 1365
Hin6I GCGC 9 cut(s) 108, 337, 421, 431, 462, 590, 835, 901, 1319
HinP1I GCGC 9 cut(s) 108, 337, 421, 431, 462, 590, 835, 901, 1319
HindIII AAGCTT 1 cut(s) 911
HphI GGTGA 3 cut(s) 535, 1228, 1309
Hpy166II GTNNAC 6 cut(s) 639, 778, 780, 841, 1000, 1105
Hpy188III TCNNGA 5 cut(s) 266, 268, 907, 973, 1400
Hpy8I GTNNAC 6 cut(s) 639, 778, 780, 841, 1000, 1105
Hpy99I CGWCG 5 cut(s) 695, 712, 854, 1319, 1348
HpyAV CCTTC 3 cut(s) 112, 1024, 1196
HpyCH4III ACNGT 5 cut(s) 295, 520, 887, 997, 1391
HpyCH4IV ACGT 5 cut(s) 1086, 1222, 1346, 1376, 1407
HpyCH4V TGCA 3 cut(s) 702, 718, 1198
HpyF3I CTNAG 3 cut(s) 37, 446, 1322
HpySE526I ACGT 5 cut(s) 1086, 1222, 1346, 1376, 1407
Hsp92I GRCGYC 4 cut(s) 337, 431, 693, 1365
HspAI GCGC 9 cut(s) 108, 337, 421, 431, 462, 590, 835, 901, 1319
KasI GGCGCC 2 cut(s) 336, 430
Lsp1109I GCAGC 2 cut(s) 537, 702
LweI GCATC 2 cut(s) 540, 612
MabI ACCWGGT 1 cut(s) 789
MaeI CTAG 1 cut(s) 644
MaeII ACGT 5 cut(s) 1086, 1222, 1346, 1376, 1407
MaeIII GTNAC 2 cut(s) 887, 1397
MbiI CCGCTC 2 cut(s) 254, 428
MboII GAAGA 5 cut(s) 607, 997, 1186, 1219, 1384
MflI RGATCY 4 cut(s) 270, 597, 1125, 1176
MhlI GDGCHC 9 cut(s) 171, 192, 209, 378, 506, 518, 737, 828, 1304
MluCI AATT 1 cut(s) 451
Mly113I GGCGCC 2 cut(s) 337, 431
MmeI TCCRAC 6 cut(s) 93, 150, 327, 558, 690, 1149
MroXI GAANNNNTTC 1 cut(s) 1223
MseI TTAA 1 cut(s) 944
MslI CAYNNNNRTG 1 cut(s) 521
MvaI CCWGG 8 cut(s) 61, 226, 310, 683, 770, 791, 1130, 1337
NarI GGCGCC 2 cut(s) 337, 431
NciI CCSGG 5 cut(s) 96, 953, 1165, 1289, 1304
NmeAIII GCCGAG 4 cut(s) 514, 535, 630, 1146
NmuCI GTSAC 1 cut(s) 1397
NspV TTCGAA 1 cut(s) 867
OliI CACNNNNGTG 1 cut(s) 521
PaeR7I CTCGAG 3 cut(s) 266, 347, 797
PaqCI CACCTGC 1 cut(s) 1234
PceI AGGCCT 1 cut(s) 152
PcsI WCGNNNNNNNCGW 4 cut(s) 291, 819, 858, 1352
PdmI GAANNNNTTC 1 cut(s) 1223
PfeI GAWTC 1 cut(s) 1208
Pfl23II CGTACG 1 cut(s) 738
PfoI TCCNGGA 2 cut(s) 1128, 1335
Ple19I CGATCG 2 cut(s) 438, 612
PluTI GGCGCC 2 cut(s) 340, 434
PpuMI RGGWCCY 1 cut(s) 931
Psp124BI GAGCTC 3 cut(s) 192, 378, 828
Psp1406I AACGTT 2 cut(s) 1222, 1407
Psp5II RGGWCCY 1 cut(s) 931
Psp6I CCWGG 8 cut(s) 59, 224, 308, 681, 768, 789, 1128, 1335
PspFI CCCAGC 2 cut(s) 47, 1090
PspGI CCWGG 8 cut(s) 59, 224, 308, 681, 768, 789, 1128, 1335
PspLI CGTACG 1 cut(s) 738
PspPI GGNCC 5 cut(s) 312, 931, 949, 1105, 1118
PspPPI RGGWCCY 1 cut(s) 931
PspXI VCTCGAGB 2 cut(s) 347, 797
PsrI GAACNNNNNNTAC 2 cut(s) 671, 703
PstNI CAGNNNCTG 1 cut(s) 1249
PsuI RGATCY 4 cut(s) 270, 597, 1125, 1176
PvuI CGATCG 2 cut(s) 438, 612
RsaI GTAC 6 cut(s) 605, 638, 740, 779, 795, 1238
RsaNI GTAC 6 cut(s) 604, 637, 739, 778, 794, 1237
RseI CAYNNNNRTG 1 cut(s) 521
SacI GAGCTC 3 cut(s) 192, 378, 828
SaqAI TTAA 1 cut(s) 944
Sau96I GGNCC 5 cut(s) 312, 931, 949, 1105, 1118
SduI GDGCHC 9 cut(s) 171, 192, 209, 378, 506, 518, 737, 828, 1304
SexAI ACCWGGT 1 cut(s) 789
SfaNI GCATC 2 cut(s) 540, 612
SfcI CTRYAG 1 cut(s) 1203
SfoI GGCGCC 2 cut(s) 338, 432
Sfr274I CTCGAG 3 cut(s) 266, 347, 797
SfuI TTCGAA 1 cut(s) 867
SinI GGWCC 3 cut(s) 931, 949, 1105
SlaI CTCGAG 3 cut(s) 266, 347, 797
SmiMI CAYNNNNRTG 1 cut(s) 521
SmlI CTYRAG 6 cut(s) 245, 266, 347, 797, 830, 971
SmoI CTYRAG 6 cut(s) 245, 266, 347, 797, 830, 971
Sse9I AATT 1 cut(s) 451
SseBI AGGCCT 1 cut(s) 152
SspDI GGCGCC 2 cut(s) 336, 430
SspMI CTAG 1 cut(s) 644
SstI GAGCTC 3 cut(s) 192, 378, 828
StuI AGGCCT 1 cut(s) 152
TaaI ACNGT 5 cut(s) 295, 520, 887, 997, 1391
TaiI ACGT 5 cut(s) 1089, 1225, 1349, 1379, 1410
TasI AATT 1 cut(s) 451
TatI WGTACW 2 cut(s) 603, 777
TfiI GAWTC 1 cut(s) 1208
Tru1I TTAA 1 cut(s) 944
Tru9I TTAA 1 cut(s) 944
TseFI GTSAC 1 cut(s) 1397
TseI GCWGC 2 cut(s) 550, 715
Tsp45I GTSAC 1 cut(s) 1397
TspGWI ACGGA 3 cut(s) 624, 1022, 1435
VpaK11BI GGWCC 3 cut(s) 931, 949, 1105
XhoI CTCGAG 3 cut(s) 266, 347, 797
XmnI GAANNNNTTC 1 cut(s) 1223
XspI CTAG 1 cut(s) 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.