Rw1G031710

Hs1pro-1 N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0013472
Physical Location & Seq
Reverse (-)
59877305 .. 59879142
1838 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G031710.1

Sequence Viewer

Length: 1440 bp
ATGGTTGATTTGGATTGGAAAGCAAAAATGGTCTCCTCAGATATACCCAGCAAGTCCCCCAGGCTCTCCAACAAGCTCCACCACCTCTCCATCCCCGCCTCCTTCCGCGCCGCCCAAATCCAGTCCGACATTTCCCCCGCCTCCGAGCAGGCCTGCTCCACCTACGAGCACTACCTCCGCCTCCCCGAGCTCCGCAAGCTCTGGTGCTCCACCGACTTCCCCGCCTGGAAAAGCGAGTCCATTCTCAAGCCCGCTCTCCAAGCTCTCGAGATCTCTTTCCGGTTCGTCTCCACCGTCCTCTCCGATCCGAGGCCCTACGCCAACCGGAGAGAGTGGCGCCGCCGCCTCGAGTCGCTTATTATTAGTCAGGTTGAGCTCATCGCTTTGTTGATCGAGGACGATGAGGAGGACAGCGAGACGCGCGGCGCGGCGCCGATCGTTGATCTGAGCACGTCCGGCGGCGCCTTGGCTCGCGACGGGAGCTTGGCCGAGGTGTGGAGGGGCTCCGGCGAGAGCACCGTGGTGAGCCGCACCAGCGAGGCCAGCCTGCTGCCTCGGCTCGCCGCGTGGCAGAAGTCGGAGTCGATGGCGCAGAAGATCCTGTACTCGATCGAGTGCGAGATGCGGAGCTGTCCGTACACTCTTGGACTCGGCGAGCCGAACCTCGCCGGAAAACCGAACCTGGATTACGACGCCGTTTGCAAGCCGTCGGAGCTGCATTTGCTAAAGAGGAGCCCGTACGACAGCCACATCGACAACCACGAGAACCAGGCGGTGTACACCACGCACCAGGTCCTGGAGTCGTGGGTGTTCGCCTGCCACGAGCTTCTCAAGCGCGTGAACCGCCGCGTCGAGTCCAGCGAATTCGAGAAGGCCGCCACCGACTGTTACCTGATCGAGCGCGTCTGGAAGCTTCTGGCGGAAATCGAGGACCTCCACCTGTTAATGGACCCGGAGGACTTTCTCCGGCTCAAGAACCAGTTGAAGATCAAGACGGTGGACGATACGGAGTCGTTTTGCTTCAGGTCGAAGGGACTGGTGGAGATCACAAGGCTGTGTAAGGACTTGAAGCACAAGGTTCCGAACGTGCTGGGAGTGGAGGTGGACCCGATGGGCGGGCCGAGGATCCAGGAGGCGGCGATGAGGCTCTACTCGGAGAAAACCGGGTCGGAGAAGATCCATCTCCTTCAGGCGATGCAGACTATAGAATCGGCCGTGAAACGTTTCTTCTTCGCGTACAAGCAGGTGCTAGTGGTGTTGATGGGGAGCTTGGAGGCGAAAGGGAACCGGGTCGTGGTGAGCCCGGAGTCCGGCGACGCGCTGAGTCAGATATTCCTGGAGCCGACGTATTTTCCGAGCTTGGACGCCGCGAAGACGTTTCTGGGGGACGGTTTGAGTCACGAAAACGTTTCGGAGAGACGGACGCGCAGGACTAGGTAG

Protein Analysis

479

Amino Acids

54.2

Weight (kDa)

6.12

Isoelectric Point (pI)

58.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hs1pro-1_N PF07231 1 - 205 9.3e-83 Hs1pro-1 N-terminus
Hs1pro-1_C PF07014 208 - 463 2.1e-134 Hs1pro-1 protein C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40000
fragaria_vesca FvH4_7g24150
malus_domestica MD01G1148800.v1.1 MD07G1215700.v1.1
prunus_persica Prupe.2G247600_v2.0.a1
pyrus_communis pycom01g16720 pycom07g19800
rosa_chinensis RchiOBHm_Chr1g0369681
rosa_laevigata RLG00000027112
rosa_multiflora Rmu_sc0001154.1_g000025
rosa_roxburghii Rroxscaffold_2G00131660 Rroxscaffold_4G00287440
rosa_rugosa Rorug01G0351800
rosa_samantha Rh1AG359900 Rh1BG323800 Rh1CG337000 Rh1DG354400
rosa_wichuraiana Rw1G031710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1234
Acc36I ACCTGC 1 cut(s) 1234
AccB1I GGYRCC 3 cut(s) 336, 430, 461
AccB7I CCANNNNNTGG 1 cut(s) 796
AccBSI CCGCTC 1 cut(s) 254
AclI AACGTT 2 cut(s) 1222, 1407
AclWI GGATC 5 cut(s) 299, 592, 1120, 1133, 1171
AcoI YGGCCR 2 cut(s) 486, 1212
AcsI RAATTY 1 cut(s) 863
AcuI CTGAAG 2 cut(s) 1006, 1172
AcyI GRCGYC 5 cut(s) 337, 431, 462, 693, 1365
AfaI GTAC 5 cut(s) 605, 638, 740, 779, 1238
AfiI CCNNNNNNNGG 8 cut(s) 309, 495, 796, 946, 1115, 1135, 1294, 1310
AgsI TTSAA 2 cut(s) 985, 1069
AjiI CACGTC 1 cut(s) 453
AjnI CCWGG 8 cut(s) 59, 224, 681, 768, 789, 795, 1128, 1335
AleI CACNNNNGTG 1 cut(s) 521
Alw21I GWGCWC 6 cut(s) 171, 192, 209, 378, 452, 518
Alw26I GTCTC 4 cut(s) 37, 292, 410, 1411
AlwI GGATC 5 cut(s) 299, 592, 1120, 1133, 1171
AlwNI CAGNNNCTG 1 cut(s) 796
Ama87I CYCGRG 3 cut(s) 185, 266, 347
AoxI GGCC 7 cut(s) 150, 311, 486, 540, 873, 1118, 1212
ApeKI GCWGC 2 cut(s) 550, 715
ApoI RAATTY 1 cut(s) 863
Asp700I GAANNNNTTC 1 cut(s) 1223
AspS9I GGNCC 6 cut(s) 312, 793, 931, 949, 1105, 1118
AsuC2I CCSGG 4 cut(s) 953, 1165, 1289, 1304
AsuHPI GGTGA 2 cut(s) 535, 1309
AvaI CYCGRG 3 cut(s) 185, 266, 347
AvaII GGWCC 4 cut(s) 793, 931, 949, 1105
BamHI GGATCC 1 cut(s) 1125
BanI GGYRCC 3 cut(s) 336, 430, 461
BanII GRGCYC 5 cut(s) 192, 378, 506, 737, 1304
BarI GAAGNNNNNNTAC 2 cut(s) 587, 619
BauI CACGAG 2 cut(s) 761, 821
BbsI GAAGAC 1 cut(s) 1379
Bbv12I GWGCWC 6 cut(s) 171, 192, 209, 378, 452, 518
BbvI GCAGC 2 cut(s) 537, 702
BccI CCATC 5 cut(s) 98, 580, 1105, 1188, 1255
BceAI ACGGC 3 cut(s) 680, 691, 1199
BciT130I CCWGG 8 cut(s) 61, 226, 683, 770, 791, 797, 1130, 1337
BcnI CCSGG 4 cut(s) 953, 1165, 1289, 1304
BcoDI GTCTC 4 cut(s) 37, 292, 410, 1411
BfaI CTAG 2 cut(s) 1250, 1434
BfmI CTRYAG 1 cut(s) 1203
BfoI RGCGCY 3 cut(s) 340, 434, 465
BfuAI ACCTGC 1 cut(s) 1234
BglII AGATCT 1 cut(s) 270
Bme18I GGWCC 4 cut(s) 793, 931, 949, 1105
BmeT110I CYCGRG 3 cut(s) 185, 266, 347
BmgBI CACGTC 1 cut(s) 453
BmgT120I GGNCC 6 cut(s) 312, 793, 931, 949, 1105, 1118
BmsI GCATC 2 cut(s) 612, 1185
BpiI GAAGAC 1 cut(s) 1379
BpmI CTGGAG 2 cut(s) 818, 1358
BpuEI CTTGAG 3 cut(s) 230, 815, 956
BpuMI CCSGG 4 cut(s) 953, 1165, 1289, 1304
BsaHI GRCGYC 5 cut(s) 337, 431, 462, 693, 1365
BsaI GGTCTC 1 cut(s) 37
BsaJI CCNNGG 7 cut(s) 59, 308, 465, 489, 519, 554, 1121
BsaWI WCCGGW 2 cut(s) 279, 324
BsaXI ACNNNNNCTCC 6 cut(s) 71, 101, 1086, 1116, 1331, 1361
Bsc4I CCNNNNNNNGG 8 cut(s) 309, 495, 796, 946, 1115, 1135, 1294, 1310
Bse1I ACTGG 3 cut(s) 121, 979, 1041
BseBI CCWGG 8 cut(s) 61, 226, 683, 770, 791, 797, 1130, 1337
BseDI CCNNGG 7 cut(s) 59, 308, 465, 489, 519, 554, 1121
BseGI GGATG 1 cut(s) 90
BseLI CCNNNNNNNGG 8 cut(s) 309, 495, 796, 946, 1115, 1135, 1294, 1310
BseMII CTCAG 3 cut(s) 51, 437, 1313
BseNI ACTGG 3 cut(s) 121, 979, 1041
BseRI GAGGAG 3 cut(s) 25, 419, 745
BseX3I CGGCCG 1 cut(s) 1212
BseXI GCAGC 2 cut(s) 537, 702
BseYI CCCAGC 2 cut(s) 47, 1090
Bsh1285I CGRYCG 3 cut(s) 438, 612, 1215
BshFI GGCC 7 cut(s) 152, 313, 488, 542, 875, 1120, 1214
BshNI GGYRCC 3 cut(s) 336, 430, 461
BsiEI CGRYCG 3 cut(s) 438, 612, 1215
BsiHKAI GWGCWC 6 cut(s) 171, 192, 209, 378, 452, 518
BsiHKCI CYCGRG 3 cut(s) 185, 266, 347
BsiWI CGTACG 1 cut(s) 738
BslFI GGGAC 3 cut(s) 40, 1047, 1400
BslI CCNNNNNNNGG 8 cut(s) 309, 495, 796, 946, 1115, 1135, 1294, 1310
BsmAI GTCTC 4 cut(s) 37, 292, 410, 1411
BsmBI CGTCTC 3 cut(s) 292, 410, 1411
BsmFI GGGAC 3 cut(s) 40, 1047, 1400
BsnI GGCC 7 cut(s) 152, 313, 488, 542, 875, 1120, 1214
Bso31I GGTCTC 1 cut(s) 37
BsoBI CYCGRG 3 cut(s) 185, 266, 347
Bsp1286I GDGCHC 9 cut(s) 171, 192, 209, 378, 452, 506, 518, 737, 1304
Bsp1407I TGTACA 1 cut(s) 777
Bsp68I TCGCGA 1 cut(s) 474
BspANI GGCC 7 cut(s) 152, 313, 488, 542, 875, 1120, 1214
BspCNI CTCAG 3 cut(s) 50, 438, 1314
BspMI ACCTGC 1 cut(s) 1234
BspPI GGATC 5 cut(s) 299, 592, 1120, 1133, 1171
BspT107I GGYRCC 3 cut(s) 336, 430, 461
BspTNI GGTCTC 1 cut(s) 37
BsrBI CCGCTC 1 cut(s) 254
BsrGI TGTACA 1 cut(s) 777
BsrI ACTGG 3 cut(s) 121, 979, 1041
BssECI CCNNGG 7 cut(s) 59, 308, 465, 489, 519, 554, 1121
BssNI GRCGYC 5 cut(s) 337, 431, 462, 693, 1365
BssSI CACGAG 2 cut(s) 761, 821
BssT1I CCWWGG 1 cut(s) 465
Bst2BI CACGAG 2 cut(s) 761, 821
Bst2UI CCWGG 8 cut(s) 61, 226, 683, 770, 791, 797, 1130, 1337
Bst4CI ACNGT 5 cut(s) 295, 520, 887, 997, 1391
BstACI GRCGYC 5 cut(s) 337, 431, 462, 693, 1365
BstAUI TGTACA 1 cut(s) 777
BstDEI CTNAG 3 cut(s) 37, 446, 1322
BstDSI CCRYGG 1 cut(s) 519
BstF5I GGATG 1 cut(s) 90
BstH2I RGCGCY 3 cut(s) 340, 434, 465
BstMAI GTCTC 4 cut(s) 37, 292, 410, 1411
BstMCI CGRYCG 3 cut(s) 438, 612, 1215
BstNI CCWGG 8 cut(s) 61, 226, 683, 770, 791, 797, 1130, 1337
BstSFI CTRYAG 1 cut(s) 1203
BstV1I GCAGC 2 cut(s) 537, 702
BstV2I GAAGAC 1 cut(s) 1379
BstX2I RGATCY 4 cut(s) 270, 597, 1125, 1176
BstYI RGATCY 4 cut(s) 270, 597, 1125, 1176
BstZI CGGCCG 1 cut(s) 1212
BsuRI GGCC 7 cut(s) 152, 313, 488, 542, 875, 1120, 1214
BtgI CCRYGG 1 cut(s) 519
BtgZI GCGATG 3 cut(s) 364, 1154, 1208
BtrI CACGTC 1 cut(s) 453
BtsCI GGATG 1 cut(s) 90
BtuMI TCGCGA 1 cut(s) 474
BveI ACCTGC 1 cut(s) 1234
CaiI CAGNNNCTG 1 cut(s) 796
Cfr13I GGNCC 6 cut(s) 312, 793, 931, 949, 1105, 1118
CseI GACGC 7 cut(s) 427, 701, 838, 892, 1325, 1373, 1432
CsiI ACCWGGT 1 cut(s) 789
Csp6I GTAC 5 cut(s) 604, 637, 739, 778, 1237
CviQI GTAC 5 cut(s) 604, 637, 739, 778, 1237
DdeI CTNAG 3 cut(s) 37, 446, 1322
DinI GGCGCC 3 cut(s) 338, 432, 463
EaeI YGGCCR 2 cut(s) 486, 1212
EagI CGGCCG 1 cut(s) 1212
EciI GGCGGA 2 cut(s) 167, 935
Ecl136II GAGCTC 2 cut(s) 190, 376
EclXI CGGCCG 1 cut(s) 1212
Eco130I CCWWGG 1 cut(s) 465
Eco147I AGGCCT 1 cut(s) 152
Eco24I GRGCYC 5 cut(s) 192, 378, 506, 737, 1304
Eco31I GGTCTC 1 cut(s) 37
Eco47I GGWCC 4 cut(s) 793, 931, 949, 1105
Eco52I CGGCCG 1 cut(s) 1212
Eco53kI GAGCTC 2 cut(s) 190, 376
Eco57I CTGAAG 2 cut(s) 1006, 1172
Eco88I CYCGRG 3 cut(s) 185, 266, 347
EcoICRI GAGCTC 2 cut(s) 190, 376
EcoO109I RGGNCCY 3 cut(s) 312, 793, 931
EcoRI GAATTC 1 cut(s) 863
EcoRII CCWGG 8 cut(s) 59, 224, 681, 768, 789, 795, 1128, 1335
EcoT14I CCWWGG 1 cut(s) 465
EcoT38I GRGCYC 5 cut(s) 192, 378, 506, 737, 1304
EgeI GGCGCC 3 cut(s) 338, 432, 463
EheI GGCGCC 3 cut(s) 338, 432, 463
ErhI CCWWGG 1 cut(s) 465
Esp3I CGTCTC 3 cut(s) 292, 410, 1411
FaiI YATR 2 cut(s) 44, 1205
FaqI GGGAC 3 cut(s) 40, 1047, 1400
FauI CCCGC 5 cut(s) 103, 145, 229, 259, 1109
FokI GGATG 1 cut(s) 77
FriOI GRGCYC 5 cut(s) 192, 378, 506, 737, 1304
FspBI CTAG 2 cut(s) 1250, 1434
GsaI CCCAGC 2 cut(s) 51, 1094
GsuI CTGGAG 2 cut(s) 818, 1358
HaeII RGCGCY 3 cut(s) 340, 434, 465
HaeIII GGCC 7 cut(s) 152, 313, 488, 542, 875, 1120, 1214
HgaI GACGC 7 cut(s) 427, 701, 838, 892, 1325, 1373, 1432
Hin1I GRCGYC 5 cut(s) 337, 431, 462, 693, 1365
HindIII AAGCTT 1 cut(s) 911
HphI GGTGA 2 cut(s) 535, 1309
Hpy166II GTNNAC 6 cut(s) 639, 778, 780, 841, 1000, 1105
Hpy188III TCNNGA 8 cut(s) 266, 268, 473, 868, 907, 973, 991, 1400
Hpy8I GTNNAC 6 cut(s) 639, 778, 780, 841, 1000, 1105
Hpy99I CGWCG 6 cut(s) 479, 695, 712, 854, 1319, 1348
HpyAV CCTTC 4 cut(s) 112, 865, 1024, 1196
HpyCH4III ACNGT 5 cut(s) 295, 520, 887, 997, 1391
HpyCH4IV ACGT 6 cut(s) 452, 1086, 1222, 1346, 1376, 1407
HpyCH4V TGCA 3 cut(s) 702, 718, 1198
HpyF3I CTNAG 3 cut(s) 37, 446, 1322
HpySE526I ACGT 6 cut(s) 452, 1086, 1222, 1346, 1376, 1407
Hsp92I GRCGYC 5 cut(s) 337, 431, 462, 693, 1365
KasI GGCGCC 3 cut(s) 336, 430, 461
Lsp1109I GCAGC 2 cut(s) 537, 702
LweI GCATC 2 cut(s) 612, 1185
MabI ACCWGGT 1 cut(s) 789
MaeI CTAG 2 cut(s) 1250, 1434
MaeII ACGT 6 cut(s) 452, 1086, 1222, 1346, 1376, 1407
MaeIII GTNAC 2 cut(s) 887, 1397
MbiI CCGCTC 1 cut(s) 254
MboII GAAGA 6 cut(s) 607, 997, 1186, 1219, 1222, 1384
MflI RGATCY 4 cut(s) 270, 597, 1125, 1176
MhlI GDGCHC 9 cut(s) 171, 192, 209, 378, 452, 506, 518, 737, 1304
MluCI AATT 1 cut(s) 863
Mly113I GGCGCC 3 cut(s) 337, 431, 462
MmeI TCCRAC 5 cut(s) 93, 150, 558, 690, 1149
MroXI GAANNNNTTC 1 cut(s) 1223
MseI TTAA 1 cut(s) 944
MslI CAYNNNNRTG 1 cut(s) 521
MteI GCGCNGCGC 2 cut(s) 424, 429
MvaI CCWGG 8 cut(s) 61, 226, 683, 770, 791, 797, 1130, 1337
NarI GGCGCC 3 cut(s) 337, 431, 462
NciI CCSGG 4 cut(s) 953, 1165, 1289, 1304
NmeAIII GCCGAG 4 cut(s) 514, 535, 630, 1146
NmuCI GTSAC 1 cut(s) 1397
NruI TCGCGA 1 cut(s) 474
OliI CACNNNNGTG 1 cut(s) 521
PaeR7I CTCGAG 2 cut(s) 266, 347
PaqCI CACCTGC 1 cut(s) 1234
PceI AGGCCT 1 cut(s) 152
PcsI WCGNNNNNNNCGW 5 cut(s) 291, 759, 819, 858, 1352
PdmI GAANNNNTTC 1 cut(s) 1223
PfeI GAWTC 1 cut(s) 1208
Pfl23II CGTACG 1 cut(s) 738
PflMI CCANNNNNTGG 1 cut(s) 796
PfoI TCCNGGA 3 cut(s) 795, 1128, 1335
Ple19I CGATCG 2 cut(s) 438, 612
PluTI GGCGCC 3 cut(s) 340, 434, 465
PpuMI RGGWCCY 2 cut(s) 793, 931
Psp124BI GAGCTC 2 cut(s) 192, 378
Psp1406I AACGTT 2 cut(s) 1222, 1407
Psp5II RGGWCCY 2 cut(s) 793, 931
Psp6I CCWGG 8 cut(s) 59, 224, 681, 768, 789, 795, 1128, 1335
PspFI CCCAGC 2 cut(s) 47, 1090
PspGI CCWGG 8 cut(s) 59, 224, 681, 768, 789, 795, 1128, 1335
PspLI CGTACG 1 cut(s) 738
PspPI GGNCC 6 cut(s) 312, 793, 931, 949, 1105, 1118
PspPPI RGGWCCY 2 cut(s) 793, 931
PspXI VCTCGAGB 1 cut(s) 347
PsrI GAACNNNNNNTAC 2 cut(s) 671, 703
PstNI CAGNNNCTG 1 cut(s) 796
PsuI RGATCY 4 cut(s) 270, 597, 1125, 1176
PvuI CGATCG 2 cut(s) 438, 612
RruI TCGCGA 1 cut(s) 474
RsaI GTAC 5 cut(s) 605, 638, 740, 779, 1238
RsaNI GTAC 5 cut(s) 604, 637, 739, 778, 1237
RseI CAYNNNNRTG 1 cut(s) 521
SacI GAGCTC 2 cut(s) 192, 378
SaqAI TTAA 1 cut(s) 944
Sau96I GGNCC 6 cut(s) 312, 793, 931, 949, 1105, 1118
SduI GDGCHC 9 cut(s) 171, 192, 209, 378, 452, 506, 518, 737, 1304
SexAI ACCWGGT 1 cut(s) 789
SfaNI GCATC 2 cut(s) 612, 1185
SfcI CTRYAG 1 cut(s) 1203
SfoI GGCGCC 3 cut(s) 338, 432, 463
Sfr274I CTCGAG 2 cut(s) 266, 347
SinI GGWCC 4 cut(s) 793, 931, 949, 1105
SlaI CTCGAG 2 cut(s) 266, 347
SmiMI CAYNNNNRTG 1 cut(s) 521
SmlI CTYRAG 5 cut(s) 245, 266, 347, 830, 971
SmoI CTYRAG 5 cut(s) 245, 266, 347, 830, 971
Sse9I AATT 1 cut(s) 863
SseBI AGGCCT 1 cut(s) 152
SspDI GGCGCC 3 cut(s) 336, 430, 461
SspMI CTAG 2 cut(s) 1250, 1434
SstI GAGCTC 2 cut(s) 192, 378
StuI AGGCCT 1 cut(s) 152
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 5 cut(s) 295, 520, 887, 997, 1391
TaiI ACGT 6 cut(s) 455, 1089, 1225, 1349, 1379, 1410
TasI AATT 1 cut(s) 863
TatI WGTACW 2 cut(s) 603, 777
TfiI GAWTC 1 cut(s) 1208
Tru1I TTAA 1 cut(s) 944
Tru9I TTAA 1 cut(s) 944
TseFI GTSAC 1 cut(s) 1397
TseI GCWGC 2 cut(s) 550, 715
Tsp45I GTSAC 1 cut(s) 1397
TspGWI ACGGA 3 cut(s) 624, 1022, 1435
Van91I CCANNNNNTGG 1 cut(s) 796
VpaK11BI GGWCC 4 cut(s) 793, 931, 949, 1105
XapI RAATTY 1 cut(s) 863
XhoI CTCGAG 2 cut(s) 266, 347
XmnI GAANNNNTTC 1 cut(s) 1223
XspI CTAG 2 cut(s) 1250, 1434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.