FvH4_7g27590

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0018081
Physical Location & Seq
Reverse (-)
20562016 .. 20562834
819 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g27590.t1

Sequence Viewer

Length: 819 bp
ATGACGACGAAGAGGTCTTCAGTTTTGCCGACGAAGATCAATGAACTCGACCGCCAAATCACCAAGGCCTTCTCTGATCAACTCGATAACCTAAGTCTCCAACTCATCCATGCAGACATCGAGCAGCGCTTGACGTTCGTGAAGAATCTTCTGTCGGCGGAGATGGCGTCACATCCGACGAAGCAGAACTACTTGGAGCATATGTGCGAAAGAGTTCGAGAACTCGAGGCGGTGTATCGCGGCTCGGGGACTGGTTCCGGTACGACAGTCTCGACGATCGACCATGTCGATAATGTTGGAGATTGTGATGAGGATGATGATGACCATGGGTCGAAGTGTTCCGGTTGCAGCGAGTCTTGTATCCAGGACGGTGGTGATGTTTCAGATGATCTTGAGTCAACGCTTGGTCAAGATACAGGAGATTGTGTGGATTTGGTTGAGGAGAAGAAAGCAATGCTGGTGACGGTGTTTGATAATGTGTTGTTTGAGGAAAAGGTTGATGTTGATCGAGTTGAAGATGAGTTTCATGATCGGGTAGAATATGTACTGTTTGATGATACGACGTCCCACAATGGCGCTTCTGTTGAAGATGGGAATGAAGAGAAGGAGTTTAGGGTTGAGGATGGGAATGGAGAGAAGGAGATTAGGGCTGAAGTGGATATGATAGAGAGTGAAAAGCAGAGTAAAGGTTTAGGGTTTCGGAAGTTATGTTGGGCGGTGGTGATGAGTGGGTTTCTGATTGGAATGGCTTTGCTGACGATGGATAGTCTTGAGTACGATGGCTACGAGGGACATGGACAGTTTCTTACTCCTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

273

Amino Acids

30.29

Weight (kDa)

4.33

Isoelectric Point (pI)

30.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7610 PF24583 9 - 80 7e-19 Domain of unknown function (DUF7610)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018081)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g27590
malus_domestica MD07G1254300.v1.1
prunus_persica Prupe.2G277600_v2.0.a1
pyrus_communis pycom01g19370
rosa_laevigata RLG00000026736
rosa_roxburghii Rroxscaffold_4G00283470
rosa_rugosa Rorug01G0383200
rosa_samantha Rh1AG394100 Rh1BG358700 Rh1CG371700 Rh1DG389300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
AatII GACGTC 1 cut(s) 566
AccII CGCG 1 cut(s) 240
AciI CCGC 5 cut(s) 52, 158, 230, 240, 716
AcuI CTGAAG 1 cut(s) 672
AcyI GRCGYC 2 cut(s) 167, 563
AfaI GTAC 3 cut(s) 262, 546, 776
AfeI AGCGCT 1 cut(s) 128
AgsI TTSAA 2 cut(s) 515, 587
AhdI GACNNNNNGTC 1 cut(s) 328
AjnI CCWGG 1 cut(s) 363
Alw26I GTCTC 2 cut(s) 101, 274
Ama87I CYCGRG 2 cut(s) 224, 244
Aor51HI AGCGCT 1 cut(s) 128
AoxI GGCC 1 cut(s) 66
ApeKI GCWGC 2 cut(s) 124, 348
Asp700I GAANNNNTTC 1 cut(s) 213
AspLEI GCGC 2 cut(s) 129, 578
AsuHPI GGTGA 4 cut(s) 52, 386, 472, 733
AvaI CYCGRG 2 cut(s) 224, 244
BaeI ACNNNNGTAYC 2 cut(s) 252, 285
BarI GAAGNNNNNNTAC 2 cut(s) 173, 205
BbsI GAAGAC 1 cut(s) 9
BbvI GCAGC 2 cut(s) 136, 360
BccI CCATC 5 cut(s) 157, 584, 617, 754, 773
BciT130I CCWGG 1 cut(s) 365
BciVI GTATCC 1 cut(s) 371
BclI TGATCA 1 cut(s) 76
BcoDI GTCTC 2 cut(s) 101, 274
BfoI RGCGCY 2 cut(s) 130, 579
BfuI GTATCC 1 cut(s) 371
BisI GCNGC 3 cut(s) 125, 241, 349
BlsI GCNGC 3 cut(s) 126, 242, 350
Bme1390I CCNGG 1 cut(s) 365
BmeRI GACNNNNNGTC 1 cut(s) 328
BmeT110I CYCGRG 2 cut(s) 224, 244
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 1 cut(s) 365
BpiI GAAGAC 1 cut(s) 9
BpuEI CTTGAG 2 cut(s) 413, 791
BsaBI GATNNNNATC 1 cut(s) 504
BsaHI GRCGYC 2 cut(s) 167, 563
BsaJI CCNNGG 2 cut(s) 63, 325
BsaWI WCCGGW 2 cut(s) 257, 341
BsaXI ACNNNNNCTCC 6 cut(s) 152, 182, 188, 218, 411, 441
Bse1I ACTGG 1 cut(s) 256
Bse3DI GCAATG 1 cut(s) 459
Bse8I GATNNNNATC 1 cut(s) 504
BseBI CCWGG 1 cut(s) 365
BseDI CCNNGG 2 cut(s) 63, 325
BseGI GGATG 4 cut(s) 105, 172, 319, 628
BseJI GATNNNNATC 1 cut(s) 504
BseMI GCAATG 1 cut(s) 459
BseNI ACTGG 1 cut(s) 256
BseRI GAGGAG 1 cut(s) 455
BseXI GCAGC 2 cut(s) 136, 360
Bsh1236I CGCG 1 cut(s) 240
Bsh1285I CGRYCG 2 cut(s) 52, 279
BshFI GGCC 1 cut(s) 68
BsiEI CGRYCG 2 cut(s) 52, 279
BsiHKCI CYCGRG 2 cut(s) 224, 244
BsiSI CCGG 2 cut(s) 258, 342
BslFI GGGAC 3 cut(s) 262, 550, 804
BsmAI GTCTC 2 cut(s) 101, 274
BsmFI GGGAC 3 cut(s) 262, 550, 804
BsnI GGCC 1 cut(s) 68
BsoBI CYCGRG 2 cut(s) 224, 244
Bsp143I GATC 6 cut(s) 36, 76, 276, 388, 505, 529
Bsp19I CCATGG 1 cut(s) 325
BspACI CCGC 5 cut(s) 52, 158, 230, 240, 716
BspANI GGCC 1 cut(s) 68
BspFNI CGCG 1 cut(s) 240
BspHI TCATGA 1 cut(s) 526
BspLI GGNNCC 1 cut(s) 256
BsrDI GCAATG 1 cut(s) 459
BsrI ACTGG 1 cut(s) 256
BssECI CCNNGG 2 cut(s) 63, 325
BssMI GATC 6 cut(s) 36, 76, 276, 388, 505, 529
BssNI GRCGYC 2 cut(s) 167, 563
BssT1I CCWWGG 2 cut(s) 63, 325
Bst2UI CCWGG 1 cut(s) 365
Bst4CI ACNGT 5 cut(s) 268, 371, 466, 549, 801
Bst6I CTCTTC 2 cut(s) 5, 594
BstACI GRCGYC 2 cut(s) 167, 563
BstDEI CTNAG 2 cut(s) 92, 816
BstDSI CCRYGG 1 cut(s) 325
BstF5I GGATG 4 cut(s) 105, 172, 319, 628
BstFNI CGCG 1 cut(s) 240
BstH2I RGCGCY 2 cut(s) 130, 579
BstHHI GCGC 2 cut(s) 129, 578
BstKTI GATC 6 cut(s) 39, 79, 279, 391, 508, 532
BstMAI GTCTC 2 cut(s) 101, 274
BstMBI GATC 6 cut(s) 36, 76, 276, 388, 505, 529
BstMCI CGRYCG 2 cut(s) 52, 279
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNI CCWGG 1 cut(s) 365
BstSCI CCNGG 1 cut(s) 363
BstUI CGCG 1 cut(s) 240
BstV1I GCAGC 2 cut(s) 136, 360
BstV2I GAAGAC 1 cut(s) 9
BstXI CCANNNNNNTGG 1 cut(s) 371
BsuI GTATCC 1 cut(s) 371
BsuRI GGCC 1 cut(s) 68
BtgI CCRYGG 1 cut(s) 325
BtsCI GGATG 4 cut(s) 105, 172, 319, 628
CciI TCATGA 1 cut(s) 526
CfoI GCGC 2 cut(s) 129, 578
CseI GACGC 1 cut(s) 156
Csp6I GTAC 3 cut(s) 261, 545, 775
CviAII CATG 5 cut(s) 110, 284, 326, 527, 794
CviJI RGCY 5 cut(s) 68, 243, 650, 749, 783
CviKI_1 RGCY 5 cut(s) 68, 243, 650, 749, 783
CviQI GTAC 3 cut(s) 261, 545, 775
DdeI CTNAG 2 cut(s) 92, 816
DpnI GATC 6 cut(s) 38, 78, 278, 390, 507, 531
DpnII GATC 6 cut(s) 36, 76, 276, 388, 505, 529
DrdI GACNNNNNNGTC 1 cut(s) 13
DriI GACNNNNNGTC 1 cut(s) 328
DseDI GACNNNNNNGTC 1 cut(s) 13
Eam1104I CTCTTC 2 cut(s) 5, 594
Eam1105I GACNNNNNGTC 1 cut(s) 328
EarI CTCTTC 2 cut(s) 5, 594
EciI GGCGGA 1 cut(s) 173
Eco130I CCWWGG 2 cut(s) 63, 325
Eco147I AGGCCT 1 cut(s) 68
Eco47III AGCGCT 1 cut(s) 128
Eco57I CTGAAG 1 cut(s) 672
Eco88I CYCGRG 2 cut(s) 224, 244
EcoRII CCWGG 1 cut(s) 363
EcoT14I CCWWGG 2 cut(s) 63, 325
ErhI CCWWGG 2 cut(s) 63, 325
FaeI CATG 5 cut(s) 113, 287, 329, 530, 797
FaqI GGGAC 3 cut(s) 262, 550, 804
FatI CATG 5 cut(s) 109, 283, 325, 526, 793
FauNDI CATATG 1 cut(s) 201
FbaI TGATCA 1 cut(s) 76
Fnu4HI GCNGC 3 cut(s) 125, 241, 349
FokI GGATG 4 cut(s) 92, 159, 326, 635
Fsp4HI GCNGC 3 cut(s) 125, 241, 349
GlaI GCGC 2 cut(s) 128, 577
GluI GCNGC 3 cut(s) 125, 241, 349
HaeII RGCGCY 2 cut(s) 130, 579
HaeIII GGCC 1 cut(s) 68
HapII CCGG 2 cut(s) 258, 342
HgaI GACGC 1 cut(s) 156
HhaI GCGC 2 cut(s) 129, 578
Hin1I GRCGYC 2 cut(s) 167, 563
Hin1II CATG 5 cut(s) 113, 287, 329, 530, 797
Hin6I GCGC 2 cut(s) 127, 576
HinP1I GCGC 2 cut(s) 127, 576
HincII GTYRAC 1 cut(s) 399
HindII GTYRAC 1 cut(s) 399
HinfI GANTC 3 cut(s) 145, 353, 395
HpaII CCGG 2 cut(s) 258, 342
HphI GGTGA 4 cut(s) 52, 386, 472, 733
Hpy166II GTNNAC 1 cut(s) 399
Hpy188I TCNGA 5 cut(s) 76, 177, 385, 702, 738
Hpy188III TCNNGA 7 cut(s) 139, 218, 271, 392, 410, 527, 770
Hpy8I GTNNAC 1 cut(s) 399
Hpy99I CGWCG 5 cut(s) 10, 34, 181, 277, 565
HpyAV CCTTC 3 cut(s) 79, 598, 631
HpyCH4III ACNGT 5 cut(s) 268, 371, 466, 549, 801
HpyCH4IV ACGT 2 cut(s) 134, 563
HpyCH4V TGCA 2 cut(s) 113, 348
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 2 cut(s) 92, 816
HpySE526I ACGT 2 cut(s) 134, 563
Hsp92I GRCGYC 2 cut(s) 167, 563
Hsp92II CATG 5 cut(s) 113, 287, 329, 530, 797
HspAI GCGC 2 cut(s) 127, 576
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 6 cut(s) 36, 76, 276, 388, 505, 529
LmnI GCTCC 1 cut(s) 196
LpnPI CCDG 7 cut(s) 237, 271, 350, 355, 377, 402, 443
Lsp1109I GCAGC 2 cut(s) 136, 360
MaeII ACGT 2 cut(s) 134, 563
MaeIII GTNAC 2 cut(s) 168, 460
MalI GATC 6 cut(s) 38, 78, 278, 390, 507, 531
MboI GATC 6 cut(s) 36, 76, 276, 388, 505, 529
MboII GAAGA 9 cut(s) 9, 22, 46, 140, 154, 457, 527, 599, 611
MlyI GAGTC 2 cut(s) 362, 404
MmeI TCCRAC 3 cut(s) 124, 200, 277
MnlI CCTC 7 cut(s) 6, 220, 304, 433, 481, 613, 781
MroXI GAANNNNTTC 1 cut(s) 213
MspI CCGG 2 cut(s) 258, 342
MspR9I CCNGG 1 cut(s) 365
MvaI CCWGG 1 cut(s) 365
MvnI CGCG 1 cut(s) 240
MwoI GCNNNNNNNGC 1 cut(s) 164
NcoI CCATGG 1 cut(s) 325
NdeI CATATG 1 cut(s) 201
NdeII GATC 6 cut(s) 36, 76, 276, 388, 505, 529
NlaIII CATG 5 cut(s) 113, 287, 329, 530, 797
NlaIV GGNNCC 1 cut(s) 256
NmuCI GTSAC 2 cut(s) 168, 460
PaeR7I CTCGAG 1 cut(s) 224
PagI TCATGA 1 cut(s) 526
PceI AGGCCT 1 cut(s) 68
PcsI WCGNNNNNNNCGW 3 cut(s) 269, 285, 783
PdmI GAANNNNTTC 1 cut(s) 213
PfeI GAWTC 1 cut(s) 145
PflFI GACNNNGTC 1 cut(s) 284
PfoI TCCNGGA 1 cut(s) 363
PkrI GCNGC 3 cut(s) 126, 242, 350
Ple19I CGATCG 1 cut(s) 279
PleI GAGTC 2 cut(s) 361, 403
PpsI GAGTC 2 cut(s) 361, 403
Psp6I CCWGG 1 cut(s) 363
PspGI CCWGG 1 cut(s) 363
PspN4I GGNNCC 1 cut(s) 256
PspXI VCTCGAGB 1 cut(s) 224
PsyI GACNNNGTC 1 cut(s) 284
PvuI CGATCG 1 cut(s) 279
RsaI GTAC 3 cut(s) 262, 546, 776
RsaNI GTAC 3 cut(s) 261, 545, 775
SatI GCNGC 3 cut(s) 125, 241, 349
Sau3AI GATC 6 cut(s) 36, 76, 276, 388, 505, 529
SchI GAGTC 2 cut(s) 362, 404
ScrFI CCNGG 1 cut(s) 365
SetI ASST 6 cut(s) 17, 93, 137, 498, 566, 691
Sfr274I CTCGAG 1 cut(s) 224
SlaI CTCGAG 1 cut(s) 224
SmlI CTYRAG 3 cut(s) 224, 392, 770
SmoI CTYRAG 3 cut(s) 224, 392, 770
SseBI AGGCCT 1 cut(s) 68
SsiI CCGC 5 cut(s) 52, 158, 230, 240, 716
StuI AGGCCT 1 cut(s) 68
StyD4I CCNGG 1 cut(s) 363
StyI CCWWGG 2 cut(s) 63, 325
TaaI ACNGT 5 cut(s) 268, 371, 466, 549, 801
TaiI ACGT 2 cut(s) 137, 566
TatI WGTACW 1 cut(s) 544
TauI GCSGC 1 cut(s) 243
TfiI GAWTC 1 cut(s) 145
TseFI GTSAC 2 cut(s) 168, 460
TseI GCWGC 2 cut(s) 124, 348
Tsp45I GTSAC 2 cut(s) 168, 460
TspDTI ATGAA 3 cut(s) 57, 515, 612
Tth111I GACNNNGTC 1 cut(s) 284
XhoI CTCGAG 1 cut(s) 224
XmnI GAANNNNTTC 1 cut(s) 213
ZraI GACGTC 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.