MD07G1254300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0018081
Physical Location & Seq
Reverse (-)
32130655 .. 32131425
771 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1254300.v1.1.491

Sequence Viewer

Length: 771 bp
ATGACGAAGAACTACAGAACTTTGCGGAAGAAACTGAAAGAACTGGAGTCGCATCTTGAAAGCGTGTTATCTCTTCCACCAGAAGAGTCGGCGGCCGAATGCCAACTTCAACACTTCGCAGACATGGCGCAGAGGCTGAGTTTTCTCGAGAAAATGCTGTCGGCGGAGAAAGCGTCTGGCCCGTCGAAGCCCAAGCACTTGGAGCACATGTCCCGGAGGATCAAGGAACTCGGTAAGATACTTTTCGAGTGGGCGGACAGTTTGGGATTAACCTCCTCCGCCATTGATTGTGACCACGTGCATGATGATCATGAAGATGTCGGCTCGACGTGTTCTTGCACCGAGTCGTGTTTGAATGACGACGGCGATGCCGCGGACGATGGCGTAAGTGGGTTGTCATCGCCGCAGCGGTACCAAGACCTAGAAGGATGTTATGAAGAGGAAGAAGAAGAGTACAAGGAACTGCCGGTAGTTTTGTTTAATGCTGATCGTGAGAATTGTAGACAAGTGATCGTGGAGGAAAAGGAAGAGACTGCGATGAAAGATGAAGGAGTAGTTGCTAAGTTAGTTGGTGTGAAGAGATCATCGGAAGAAGGAGAGAGGAAGAGTGGTGGGAGTAATGGAGGTGTTGGGGTGGGGATGATATGTGGGGCGGTGGTGACAGGGTGGTTCCTTGGGATCGTTTCGGCCGTCTTGGCGGGGTTGTCTCCTGGAGGTTTCCTGTTTCAATGTGGATCACATCAATATGGGAATTTTCTCACTCCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

257

Amino Acids

27.95

Weight (kDa)

4.8

Isoelectric Point (pI)

47.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7610 PF24583 8 - 87 6.3e-21 Domain of unknown function (DUF7610)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018081)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g27590
malus_domestica MD07G1254300.v1.1
prunus_persica Prupe.2G277600_v2.0.a1
pyrus_communis pycom01g19370
rosa_laevigata RLG00000026736
rosa_roxburghii Rroxscaffold_4G00283470
rosa_rugosa Rorug01G0383200
rosa_samantha Rh1AG394100 Rh1BG358700 Rh1CG371700 Rh1DG389300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 411
AccB1I GGYRCC 1 cut(s) 411
AccI GTMKAC 1 cut(s) 502
AccII CGCG 1 cut(s) 374
AclWI GGATC 3 cut(s) 227, 686, 742
AcoI YGGCCR 2 cut(s) 93, 687
AcsI RAATTY 1 cut(s) 751
AcvI CACGTG 1 cut(s) 298
AfaI GTAC 2 cut(s) 413, 455
AflIII ACRYGT 2 cut(s) 207, 329
AgsI TTSAA 4 cut(s) 59, 110, 355, 728
AjiI CACGTC 1 cut(s) 330
AjnI CCWGG 1 cut(s) 709
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 2 cut(s) 524, 711
AlwI GGATC 3 cut(s) 227, 686, 742
AlwNI CAGNNNCTG 1 cut(s) 136
Ama87I CYCGRG 1 cut(s) 146
AoxI GGCC 3 cut(s) 93, 178, 687
ApeKI GCWGC 1 cut(s) 406
ApoI RAATTY 1 cut(s) 751
Asp718I GGTACC 1 cut(s) 411
AspLEI GCGC 1 cut(s) 130
AspS9I GGNCC 1 cut(s) 179
AsuC2I CCSGG 1 cut(s) 214
AsuHPI GGTGA 1 cut(s) 670
AvaI CYCGRG 1 cut(s) 146
BanI GGYRCC 1 cut(s) 411
BbrPI CACGTG 1 cut(s) 298
Bbv12I GWGCWC 1 cut(s) 207
BbvI GCAGC 1 cut(s) 418
BccI CCATC 1 cut(s) 374
BceAI ACGGC 2 cut(s) 379, 674
BcgI CGANNNNNNTGC 2 cut(s) 350, 384
BciT130I CCWGG 1 cut(s) 711
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 1 cut(s) 214
BcoDI GTCTC 2 cut(s) 524, 711
BfaI CTAG 1 cut(s) 422
BfmI CTRYAG 1 cut(s) 13
BglI GCCNNNNNGGC 1 cut(s) 695
BisI GCNGC 4 cut(s) 93, 372, 404, 407
BlsI GCNGC 4 cut(s) 94, 373, 405, 408
Bme1390I CCNGG 2 cut(s) 214, 711
BmeT110I CYCGRG 1 cut(s) 146
BmgBI CACGTC 1 cut(s) 330
BmgT120I GGNCC 1 cut(s) 179
BmiI GGNNCC 2 cut(s) 413, 671
BmrFI CCNGG 2 cut(s) 214, 711
BmsI GCATC 2 cut(s) 61, 358
BpmI CTGGAG 2 cut(s) 65, 732
BpuMI CCSGG 1 cut(s) 214
BsaAI YACGTR 1 cut(s) 298
BsaJI CCNNGG 2 cut(s) 372, 673
BsaXI ACNNNNNCTCC 4 cut(s) 158, 188, 194, 224
Bse118I RCCGGY 1 cut(s) 466
Bse1I ACTGG 1 cut(s) 48
BseBI CCWGG 1 cut(s) 711
BseDI CCNNGG 2 cut(s) 372, 673
BseGI GGATG 2 cut(s) 434, 645
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 1 cut(s) 48
BseRI GAGGAG 1 cut(s) 265
BseX3I CGGCCG 2 cut(s) 93, 687
BseXI GCAGC 1 cut(s) 418
Bsh1236I CGCG 1 cut(s) 374
Bsh1285I CGRYCG 2 cut(s) 96, 690
BshFI GGCC 3 cut(s) 95, 180, 689
BshNI GGYRCC 1 cut(s) 411
BsiEI CGRYCG 2 cut(s) 96, 690
BsiHKAI GWGCWC 1 cut(s) 207
BsiHKCI CYCGRG 1 cut(s) 146
BsiSI CCGG 2 cut(s) 214, 467
BslFI GGGAC 1 cut(s) 196
BsmAI GTCTC 2 cut(s) 524, 711
BsmFI GGGAC 1 cut(s) 196
BsmI GAATGC 1 cut(s) 104
BsnI GGCC 3 cut(s) 95, 180, 689
BsoBI CYCGRG 1 cut(s) 146
Bsp1286I GDGCHC 1 cut(s) 207
Bsp143I GATC 7 cut(s) 219, 307, 487, 510, 581, 678, 734
BspANI GGCC 3 cut(s) 95, 180, 689
BspCNI CTCAG 1 cut(s) 129
BspFNI CGCG 1 cut(s) 374
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 2 cut(s) 413, 671
BspPI GGATC 3 cut(s) 227, 686, 742
BspT107I GGYRCC 1 cut(s) 411
BsrFI RCCGGY 1 cut(s) 466
BsrI ACTGG 1 cut(s) 48
BssAI RCCGGY 1 cut(s) 466
BssECI CCNNGG 2 cut(s) 372, 673
BssMI GATC 7 cut(s) 219, 307, 487, 510, 581, 678, 734
BssT1I CCWWGG 1 cut(s) 673
Bst2UI CCWGG 1 cut(s) 711
Bst4CI ACNGT 1 cut(s) 260
Bst6I CTCTTC 7 cut(s) 78, 78, 432, 444, 522, 572, 599
BstBAI YACGTR 1 cut(s) 298
BstDEI CTNAG 2 cut(s) 137, 561
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 2 cut(s) 434, 645
BstFNI CGCG 1 cut(s) 374
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 7 cut(s) 222, 310, 490, 513, 584, 681, 737
BstMAI GTCTC 2 cut(s) 524, 711
BstMBI GATC 7 cut(s) 219, 307, 487, 510, 581, 678, 734
BstMCI CGRYCG 2 cut(s) 96, 690
BstMWI GCNNNNNNNGC 4 cut(s) 125, 170, 202, 695
BstNI CCWGG 1 cut(s) 711
BstNSI RCATGY 1 cut(s) 211
BstSCI CCNGG 2 cut(s) 212, 709
BstSFI CTRYAG 1 cut(s) 13
BstUI CGCG 1 cut(s) 374
BstV1I GCAGC 1 cut(s) 418
BstXI CCANNNNNNTGG 1 cut(s) 199
BstZI CGGCCG 2 cut(s) 93, 687
BsuRI GGCC 3 cut(s) 95, 180, 689
BtgI CCRYGG 1 cut(s) 372
BtgZI GCGATG 3 cut(s) 381, 384, 551
BtrI CACGTC 1 cut(s) 330
BtsCI GGATG 2 cut(s) 434, 645
CaiI CAGNNNCTG 1 cut(s) 136
CciI TCATGA 1 cut(s) 310
CfoI GCGC 1 cut(s) 130
Cfr10I RCCGGY 1 cut(s) 466
Cfr13I GGNCC 1 cut(s) 179
Cfr42I CCGCGG 1 cut(s) 375
CseI GACGC 1 cut(s) 162
Csp6I GTAC 2 cut(s) 412, 454
CviAII CATG 4 cut(s) 124, 208, 302, 311
CviJI RGCY 6 cut(s) 95, 136, 180, 190, 324, 689
CviKI_1 RGCY 6 cut(s) 95, 136, 180, 190, 324, 689
CviQI GTAC 2 cut(s) 412, 454
DdeI CTNAG 2 cut(s) 137, 561
DpnI GATC 7 cut(s) 221, 309, 489, 512, 583, 680, 736
DpnII GATC 7 cut(s) 219, 307, 487, 510, 581, 678, 734
EaeI YGGCCR 2 cut(s) 93, 687
EagI CGGCCG 2 cut(s) 93, 687
Eam1104I CTCTTC 7 cut(s) 78, 78, 432, 444, 522, 572, 599
EarI CTCTTC 7 cut(s) 78, 78, 432, 444, 522, 572, 599
EciI GGCGGA 3 cut(s) 179, 268, 269
EclXI CGGCCG 2 cut(s) 93, 687
Eco130I CCWWGG 1 cut(s) 673
Eco52I CGGCCG 2 cut(s) 93, 687
Eco72I CACGTG 1 cut(s) 298
Eco88I CYCGRG 1 cut(s) 146
EcoRII CCWGG 1 cut(s) 709
EcoT14I CCWWGG 1 cut(s) 673
ErhI CCWWGG 1 cut(s) 673
FaeI CATG 4 cut(s) 127, 211, 305, 314
FaiI YATR 7 cut(s) 125, 209, 303, 312, 435, 646, 747
FaqI GGGAC 1 cut(s) 196
FatI CATG 4 cut(s) 123, 207, 301, 310
FauI CCCGC 1 cut(s) 691
FbaI TGATCA 1 cut(s) 307
FblI GTMKAC 1 cut(s) 502
Fnu4HI GCNGC 4 cut(s) 93, 372, 404, 407
FokI GGATG 2 cut(s) 441, 652
Fsp4HI GCNGC 4 cut(s) 93, 372, 404, 407
FspBI CTAG 1 cut(s) 422
GlaI GCGC 1 cut(s) 129
GluI GCNGC 4 cut(s) 93, 372, 404, 407
GsuI CTGGAG 2 cut(s) 65, 732
HaeIII GGCC 3 cut(s) 95, 180, 689
HapII CCGG 2 cut(s) 214, 467
HgaI GACGC 1 cut(s) 162
HhaI GCGC 1 cut(s) 130
Hin1II CATG 4 cut(s) 127, 211, 305, 314
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HinfI GANTC 3 cut(s) 47, 86, 344
HpaII CCGG 2 cut(s) 214, 467
HphI GGTGA 1 cut(s) 670
Hpy166II GTNNAC 1 cut(s) 503
Hpy188I TCNGA 1 cut(s) 589
Hpy188III TCNNGA 5 cut(s) 56, 146, 148, 311, 491
Hpy8I GTNNAC 1 cut(s) 503
Hpy99I CGWCG 3 cut(s) 187, 331, 365
HpyAV CCTTC 3 cut(s) 419, 542, 587
HpyCH4III ACNGT 1 cut(s) 260
HpyCH4IV ACGT 2 cut(s) 297, 329
HpyCH4V TGCA 2 cut(s) 301, 339
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 170, 202, 695
HpyF3I CTNAG 2 cut(s) 137, 561
HpySE526I ACGT 2 cut(s) 297, 329
Hsp92II CATG 4 cut(s) 127, 211, 305, 314
HspAI GCGC 1 cut(s) 128
KpnI GGTACC 1 cut(s) 415
Ksp22I TGATCA 1 cut(s) 307
KspI CCGCGG 1 cut(s) 375
Kzo9I GATC 7 cut(s) 219, 307, 487, 510, 581, 678, 734
LmnI GCTCC 1 cut(s) 202
LpnPI CCDG 9 cut(s) 29, 93, 162, 227, 480, 648, 696, 723, 734
Lsp1109I GCAGC 1 cut(s) 418
LweI GCATC 2 cut(s) 61, 358
MaeI CTAG 1 cut(s) 422
MaeII ACGT 2 cut(s) 297, 329
MaeIII GTNAC 2 cut(s) 290, 658
MalI GATC 7 cut(s) 221, 309, 489, 512, 583, 680, 736
MboI GATC 7 cut(s) 219, 307, 487, 510, 581, 678, 734
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 2 cut(s) 496, 751
MlyI GAGTC 3 cut(s) 56, 95, 353
MnlI CCTC 9 cut(s) 126, 210, 283, 286, 433, 511, 594, 617, 707
MseI TTAA 3 cut(s) 269, 480, 769
MslI CAYNNNNRTG 3 cut(s) 300, 315, 744
MspA1I CMGCKG 2 cut(s) 374, 409
MspI CCGG 2 cut(s) 214, 467
MspR9I CCNGG 2 cut(s) 214, 711
Mva1269I GAATGC 1 cut(s) 104
MvaI CCWGG 1 cut(s) 711
MvnI CGCG 1 cut(s) 374
MwoI GCNNNNNNNGC 4 cut(s) 125, 170, 202, 695
NciI CCSGG 1 cut(s) 214
NdeII GATC 7 cut(s) 219, 307, 487, 510, 581, 678, 734
NlaIII CATG 4 cut(s) 127, 211, 305, 314
NlaIV GGNNCC 2 cut(s) 413, 671
NmuCI GTSAC 2 cut(s) 290, 658
NspI RCATGY 1 cut(s) 211
PaeR7I CTCGAG 1 cut(s) 146
PagI TCATGA 1 cut(s) 310
PciI ACATGT 1 cut(s) 207
PcsI WCGNNNNNNNCGW 1 cut(s) 687
PctI GAATGC 1 cut(s) 104
PfoI TCCNGGA 2 cut(s) 212, 709
PkrI GCNGC 4 cut(s) 94, 373, 405, 408
PleI GAGTC 3 cut(s) 55, 94, 352
PmaCI CACGTG 1 cut(s) 298
PmlI CACGTG 1 cut(s) 298
PpsI GAGTC 3 cut(s) 55, 94, 352
Ppu21I YACGTR 1 cut(s) 298
PscI ACATGT 1 cut(s) 207
Psp6I CCWGG 1 cut(s) 709
PspCI CACGTG 1 cut(s) 298
PspGI CCWGG 1 cut(s) 709
PspN4I GGNNCC 2 cut(s) 413, 671
PspPI GGNCC 1 cut(s) 179
PstNI CAGNNNCTG 1 cut(s) 136
RsaI GTAC 2 cut(s) 413, 455
RsaNI GTAC 2 cut(s) 412, 454
RseI CAYNNNNRTG 3 cut(s) 300, 315, 744
SacII CCGCGG 1 cut(s) 375
SaqAI TTAA 3 cut(s) 269, 480, 769
SatI GCNGC 4 cut(s) 93, 372, 404, 407
Sau3AI GATC 7 cut(s) 219, 307, 487, 510, 581, 678, 734
Sau96I GGNCC 1 cut(s) 179
SchI GAGTC 3 cut(s) 56, 95, 353
ScrFI CCNGG 2 cut(s) 214, 711
SduI GDGCHC 1 cut(s) 207
SetI ASST 6 cut(s) 275, 300, 332, 423, 628, 718
SfaNI GCATC 2 cut(s) 61, 358
SfcI CTRYAG 1 cut(s) 13
Sfr274I CTCGAG 1 cut(s) 146
Sfr303I CCGCGG 1 cut(s) 375
SgrBI CCGCGG 1 cut(s) 375
SlaI CTCGAG 1 cut(s) 146
SmiMI CAYNNNNRTG 3 cut(s) 300, 315, 744
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
Sse9I AATT 2 cut(s) 496, 751
SspMI CTAG 1 cut(s) 422
StyD4I CCNGG 2 cut(s) 212, 709
StyI CCWWGG 1 cut(s) 673
TaaI ACNGT 1 cut(s) 260
TaiI ACGT 2 cut(s) 300, 332
TaqI TCGA 4 cut(s) 147, 185, 246, 326
TasI AATT 2 cut(s) 496, 751
TatI WGTACW 1 cut(s) 453
TauI GCSGC 3 cut(s) 95, 374, 406
Tru1I TTAA 3 cut(s) 269, 480, 769
Tru9I TTAA 3 cut(s) 269, 480, 769
TseFI GTSAC 2 cut(s) 290, 658
TseI GCWGC 1 cut(s) 406
Tsp45I GTSAC 2 cut(s) 290, 658
TspDTI ATGAA 4 cut(s) 327, 450, 554, 561
XapI RAATTY 1 cut(s) 751
XceI RCATGY 1 cut(s) 211
XhoI CTCGAG 1 cut(s) 146
XmiI GTMKAC 1 cut(s) 502
XspI CTAG 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.