pycom01g19370

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0018081
Physical Location & Seq
Reverse (-)
18048646 .. 18049425
780 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g19370.1

Sequence Viewer

Length: 780 bp
ATGACGAAGACATACAGAATTTTGCGGAAGAAACTGAAAGAACTGGAGTCGTATCTCGAAAGCGTGTTAGCTCTTCCACCGGAAGAGTTGGCGGCAGAATGCCAACTTCATCACTTCCTAGATATGGCGCAAAGGCTGAGTTTTCTCGAGAAAATGCTGTCGGCGGAGAAAGCGTCTAGCCCGTCGAAGCCCAAGCACTTGGAACACATGTCCCGGAGGCTCAAGGAACTCGGGAAGATACTTTCCGAGTGGGTAGACAGTTTGGGCTTAACCTCTTCCGGCATGGATTGTGAACACGTGCGTGATGATCATGAAGATGTCGGTTCGACGTGTTCTTGTACCGAGTCGTGCTTGAATGACGACGGCGATGCCACTGCAGACGATGGAGTCGATGGGTTGTCATCCCCGCAGCGGTACCAAGACCTAGAAGGATGTTATGAAGAAAAGGAAGAAATACAAGAAGAAGAGTACAAGGAACTGCCGGTGGTTTTGTTTGGTCGTGAGAATCGTAGTCAAGTGATCGTGGAGGAGAAGGAAGAGACTGATGGAGGAGTAGTTGCTAAGTTAGTTGATGTGAAGAGATCTTCATCATCGGCGTCAGAAGGAGAGAGGAAGAGTGGTGGGAGTAACGGAGGTGTTGGGGTGAGGATGATGTGTGGGGCGGTGGTGATAGGGTGGTTGCTTGGGATGGTTTCTGCCGTCGTGGCGGGGTTGACTCCTGGAGGTTTTCTGTTTCAACGTGGATCACAGCAATATGGGAATTTTCTCACTCCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

260

Amino Acids

28.46

Weight (kDa)

4.86

Isoelectric Point (pI)

52.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7610 PF24583 8 - 86 3.8e-21 Domain of unknown function (DUF7610)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018081)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g27590
malus_domestica MD07G1254300.v1.1
prunus_persica Prupe.2G277600_v2.0.a1
pyrus_communis pycom01g19370
rosa_laevigata RLG00000026736
rosa_roxburghii Rroxscaffold_4G00283470
rosa_rugosa Rorug01G0383200
rosa_samantha Rh1AG394100 Rh1BG358700 Rh1CG371700 Rh1DG389300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 386
Acc65I GGTACC 1 cut(s) 414
AccB1I GGYRCC 1 cut(s) 414
AccI GTMKAC 1 cut(s) 255
AciI CCGC 7 cut(s) 25, 92, 164, 407, 412, 662, 707
AclWI GGATC 1 cut(s) 751
AcsI RAATTY 2 cut(s) 18, 760
AcvI CACGTG 1 cut(s) 298
AcyI GRCGYC 1 cut(s) 596
AfaI GTAC 3 cut(s) 340, 416, 470
AfiI CCNNNNNNNGG 2 cut(s) 124, 411
AflIII ACRYGT 3 cut(s) 207, 295, 329
AgsI TTSAA 2 cut(s) 355, 737
AjiI CACGTC 1 cut(s) 330
AjnI CCWGG 1 cut(s) 718
AleI CACNNNNGTG 1 cut(s) 300
AluBI AGCT 1 cut(s) 71
AluI AGCT 1 cut(s) 71
Alw26I GTCTC 1 cut(s) 533
AlwI GGATC 1 cut(s) 751
Ama87I CYCGRG 2 cut(s) 146, 230
ApeKI GCWGC 1 cut(s) 409
ApoI RAATTY 2 cut(s) 18, 760
Asp718I GGTACC 1 cut(s) 414
AspLEI GCGC 1 cut(s) 130
AsuC2I CCSGG 1 cut(s) 214
AsuHPI GGTGA 2 cut(s) 655, 679
AvaI CYCGRG 2 cut(s) 146, 230
BanI GGYRCC 1 cut(s) 414
BbrPI CACGTG 1 cut(s) 298
BbsI GAAGAC 1 cut(s) 14
BbvI GCAGC 1 cut(s) 421
BccI CCATC 4 cut(s) 377, 386, 539, 682
BceAI ACGGC 2 cut(s) 379, 683
BcgI CGANNNNNNTGC 4 cut(s) 350, 356, 384, 390
BciT130I CCWGG 1 cut(s) 720
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 1 cut(s) 214
BcoDI GTCTC 1 cut(s) 533
BfaI CTAG 3 cut(s) 119, 177, 425
BfmI CTRYAG 1 cut(s) 375
BglI GCCNNNNNGGC 1 cut(s) 704
BglII AGATCT 1 cut(s) 581
BisI GCNGC 2 cut(s) 93, 410
BlsI GCNGC 2 cut(s) 94, 411
Bme1390I CCNGG 2 cut(s) 214, 720
BmeT110I CYCGRG 2 cut(s) 146, 230
BmgBI CACGTC 1 cut(s) 330
BmiI GGNNCC 1 cut(s) 416
BmrFI CCNGG 2 cut(s) 214, 720
BmsI GCATC 1 cut(s) 358
BpiI GAAGAC 1 cut(s) 14
BpmI CTGGAG 2 cut(s) 65, 741
BpuEI CTTGAG 1 cut(s) 206
BpuMI CCSGG 1 cut(s) 214
BsaAI YACGTR 1 cut(s) 298
BsaBI GATNNNNATC 1 cut(s) 586
BsaHI GRCGYC 1 cut(s) 596
BsaWI WCCGGW 1 cut(s) 79
BsaXI ACNNNNNCTCC 4 cut(s) 158, 188, 540, 570
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 411
Bse118I RCCGGY 1 cut(s) 481
Bse1I ACTGG 1 cut(s) 48
Bse8I GATNNNNATC 1 cut(s) 586
BseBI CCWGG 1 cut(s) 720
BseGI GGATG 4 cut(s) 401, 437, 654, 693
BseJI GATNNNNATC 1 cut(s) 586
BseLI CCNNNNNNNGG 2 cut(s) 124, 411
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 1 cut(s) 48
BseRI GAGGAG 2 cut(s) 542, 564
BseXI GCAGC 1 cut(s) 421
BshNI GGYRCC 1 cut(s) 414
BsiHKCI CYCGRG 2 cut(s) 146, 230
BsiSI CCGG 4 cut(s) 80, 214, 279, 482
BslFI GGGAC 1 cut(s) 196
BslI CCNNNNNNNGG 2 cut(s) 124, 411
BsmAI GTCTC 1 cut(s) 533
BsmFI GGGAC 1 cut(s) 196
BsmI GAATGC 1 cut(s) 104
BsoBI CYCGRG 2 cut(s) 146, 230
Bsp143I GATC 4 cut(s) 307, 519, 581, 743
BspACI CCGC 7 cut(s) 25, 92, 164, 407, 412, 662, 707
BspCNI CTCAG 1 cut(s) 129
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 1 cut(s) 416
BspMAI CTGCAG 1 cut(s) 379
BspPI GGATC 1 cut(s) 751
BspQI GCTCTTC 1 cut(s) 78
BspT107I GGYRCC 1 cut(s) 414
BsrFI RCCGGY 1 cut(s) 481
BsrI ACTGG 1 cut(s) 48
BssAI RCCGGY 1 cut(s) 481
BssMI GATC 4 cut(s) 307, 519, 581, 743
BssNI GRCGYC 1 cut(s) 596
Bst2UI CCWGG 1 cut(s) 720
Bst4CI ACNGT 1 cut(s) 260
Bst6I CTCTTC 7 cut(s) 78, 78, 280, 459, 531, 572, 608
BstACI GRCGYC 1 cut(s) 596
BstBAI YACGTR 1 cut(s) 298
BstDEI CTNAG 2 cut(s) 137, 561
BstF5I GGATG 4 cut(s) 401, 437, 654, 693
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 4 cut(s) 310, 522, 584, 746
BstMAI GTCTC 1 cut(s) 533
BstMBI GATC 4 cut(s) 307, 519, 581, 743
BstMWI GCNNNNNNNGC 2 cut(s) 170, 704
BstNI CCWGG 1 cut(s) 720
BstNSI RCATGY 1 cut(s) 211
BstSCI CCNGG 2 cut(s) 212, 718
BstSFI CTRYAG 1 cut(s) 375
BstV1I GCAGC 1 cut(s) 421
BstV2I GAAGAC 1 cut(s) 14
BstX2I RGATCY 1 cut(s) 581
BstXI CCANNNNNNTGG 1 cut(s) 199
BstYI RGATCY 1 cut(s) 581
BtgZI GCGATG 1 cut(s) 381
BtrI CACGTC 1 cut(s) 330
BtsCI GGATG 4 cut(s) 401, 437, 654, 693
BtsI GCAGTG 1 cut(s) 372
BtsIMutI CAGTG 1 cut(s) 372
CciI TCATGA 1 cut(s) 310
CfoI GCGC 1 cut(s) 130
Cfr10I RCCGGY 1 cut(s) 481
CseI GACGC 2 cut(s) 162, 585
Csp6I GTAC 3 cut(s) 339, 415, 469
CviAII CATG 3 cut(s) 208, 283, 311
CviJI RGCY 6 cut(s) 71, 136, 180, 190, 220, 267
CviKI_1 RGCY 6 cut(s) 71, 136, 180, 190, 220, 267
CviQI GTAC 3 cut(s) 339, 415, 469
DdeI CTNAG 2 cut(s) 137, 561
DpnI GATC 4 cut(s) 309, 521, 583, 745
DpnII GATC 4 cut(s) 307, 519, 581, 743
DrdI GACNNNNNNGTC 1 cut(s) 386
DseDI GACNNNNNNGTC 1 cut(s) 386
Eam1104I CTCTTC 7 cut(s) 78, 78, 280, 459, 531, 572, 608
EarI CTCTTC 7 cut(s) 78, 78, 280, 459, 531, 572, 608
EciI GGCGGA 1 cut(s) 179
Eco72I CACGTG 1 cut(s) 298
Eco88I CYCGRG 2 cut(s) 146, 230
EcoRII CCWGG 1 cut(s) 718
FaeI CATG 3 cut(s) 211, 286, 314
FaiI YATR 7 cut(s) 13, 125, 209, 284, 312, 438, 756
FaqI GGGAC 1 cut(s) 196
FatI CATG 3 cut(s) 207, 282, 310
FauI CCCGC 2 cut(s) 414, 700
FbaI TGATCA 1 cut(s) 307
FblI GTMKAC 1 cut(s) 255
Fnu4HI GCNGC 2 cut(s) 93, 410
FokI GGATG 4 cut(s) 388, 444, 661, 700
Fsp4HI GCNGC 2 cut(s) 93, 410
FspBI CTAG 3 cut(s) 119, 177, 425
GlaI GCGC 1 cut(s) 129
GluI GCNGC 2 cut(s) 93, 410
GsuI CTGGAG 2 cut(s) 65, 741
HapII CCGG 4 cut(s) 80, 214, 279, 482
HgaI GACGC 2 cut(s) 162, 585
HhaI GCGC 1 cut(s) 130
Hin1I GRCGYC 1 cut(s) 596
Hin1II CATG 3 cut(s) 211, 286, 314
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HincII GTYRAC 1 cut(s) 714
HindII GTYRAC 1 cut(s) 714
HinfI GANTC 5 cut(s) 47, 344, 387, 505, 715
HpaII CCGG 4 cut(s) 80, 214, 279, 482
HphI GGTGA 2 cut(s) 655, 679
Hpy166II GTNNAC 3 cut(s) 256, 293, 714
Hpy188I TCNGA 2 cut(s) 247, 601
Hpy188III TCNNGA 6 cut(s) 56, 146, 148, 232, 311, 500
Hpy8I GTNNAC 3 cut(s) 256, 293, 714
Hpy99I CGWCG 4 cut(s) 187, 331, 365, 704
HpyAV CCTTC 3 cut(s) 422, 526, 596
HpyCH4III ACNGT 1 cut(s) 260
HpyCH4IV ACGT 3 cut(s) 297, 329, 739
HpyCH4V TGCA 1 cut(s) 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 704
HpyF3I CTNAG 2 cut(s) 137, 561
HpySE526I ACGT 3 cut(s) 297, 329, 739
Hsp92I GRCGYC 1 cut(s) 596
Hsp92II CATG 3 cut(s) 211, 286, 314
HspAI GCGC 1 cut(s) 128
KpnI GGTACC 1 cut(s) 418
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 4 cut(s) 307, 519, 581, 743
LguI GCTCTTC 1 cut(s) 78
LpnPI CCDG 7 cut(s) 29, 93, 227, 292, 495, 705, 732
Lsp1109I GCAGC 1 cut(s) 421
LweI GCATC 1 cut(s) 358
MaeI CTAG 3 cut(s) 119, 177, 425
MaeII ACGT 3 cut(s) 297, 329, 739
MaeIII GTNAC 1 cut(s) 626
MalI GATC 4 cut(s) 309, 521, 583, 745
MboI GATC 4 cut(s) 307, 519, 581, 743
MflI RGATCY 1 cut(s) 581
MluCI AATT 2 cut(s) 18, 760
MlyI GAGTC 4 cut(s) 56, 353, 396, 709
MnlI CCTC 8 cut(s) 210, 283, 520, 542, 603, 626, 639, 716
MseI TTAA 2 cut(s) 269, 778
MslI CAYNNNNRTG 2 cut(s) 300, 315
MspA1I CMGCKG 1 cut(s) 412
MspI CCGG 4 cut(s) 80, 214, 279, 482
MspR9I CCNGG 2 cut(s) 214, 720
Mva1269I GAATGC 1 cut(s) 104
MvaI CCWGG 1 cut(s) 720
MwoI GCNNNNNNNGC 2 cut(s) 170, 704
NciI CCSGG 1 cut(s) 214
NdeII GATC 4 cut(s) 307, 519, 581, 743
NlaIII CATG 3 cut(s) 211, 286, 314
NlaIV GGNNCC 1 cut(s) 416
NspI RCATGY 1 cut(s) 211
OliI CACNNNNGTG 1 cut(s) 300
PaeR7I CTCGAG 1 cut(s) 146
PagI TCATGA 1 cut(s) 310
PciI ACATGT 1 cut(s) 207
PciSI GCTCTTC 1 cut(s) 78
PcsI WCGNNNNNNNCGW 2 cut(s) 387, 505
PctI GAATGC 1 cut(s) 104
PfeI GAWTC 1 cut(s) 505
PfoI TCCNGGA 2 cut(s) 212, 718
PkrI GCNGC 2 cut(s) 94, 411
PleI GAGTC 4 cut(s) 55, 352, 395, 709
PmaCI CACGTG 1 cut(s) 298
PmlI CACGTG 1 cut(s) 298
PpsI GAGTC 4 cut(s) 55, 352, 395, 709
Ppu21I YACGTR 1 cut(s) 298
PscI ACATGT 1 cut(s) 207
Psp6I CCWGG 1 cut(s) 718
PspCI CACGTG 1 cut(s) 298
PspGI CCWGG 1 cut(s) 718
PspN4I GGNNCC 1 cut(s) 416
PstI CTGCAG 1 cut(s) 379
PsuI RGATCY 1 cut(s) 581
RsaI GTAC 3 cut(s) 340, 416, 470
RsaNI GTAC 3 cut(s) 339, 415, 469
RseI CAYNNNNRTG 2 cut(s) 300, 315
SapI GCTCTTC 1 cut(s) 78
SaqAI TTAA 2 cut(s) 269, 778
SatI GCNGC 2 cut(s) 93, 410
Sau3AI GATC 4 cut(s) 307, 519, 581, 743
SchI GAGTC 4 cut(s) 56, 353, 396, 709
ScrFI CCNGG 2 cut(s) 214, 720
SetI ASST 8 cut(s) 73, 275, 300, 332, 426, 637, 727, 742
SfaNI GCATC 1 cut(s) 358
SfcI CTRYAG 1 cut(s) 375
Sfr274I CTCGAG 1 cut(s) 146
SlaI CTCGAG 1 cut(s) 146
SmiMI CAYNNNNRTG 2 cut(s) 300, 315
SmlI CTYRAG 2 cut(s) 146, 221
SmoI CTYRAG 2 cut(s) 146, 221
Sse9I AATT 2 cut(s) 18, 760
SsiI CCGC 7 cut(s) 25, 92, 164, 407, 412, 662, 707
SspMI CTAG 3 cut(s) 119, 177, 425
StyD4I CCNGG 2 cut(s) 212, 718
TaaI ACNGT 1 cut(s) 260
TaiI ACGT 3 cut(s) 300, 332, 742
TaqI TCGA 5 cut(s) 57, 147, 185, 326, 390
TasI AATT 2 cut(s) 18, 760
TatI WGTACW 1 cut(s) 468
TauI GCSGC 1 cut(s) 95
TfiI GAWTC 1 cut(s) 505
Tru1I TTAA 2 cut(s) 269, 778
Tru9I TTAA 2 cut(s) 269, 778
TscAI CASTG 1 cut(s) 379
TseI GCWGC 1 cut(s) 409
TspDTI ATGAA 4 cut(s) 98, 327, 453, 576
TspGWI ACGGA 1 cut(s) 645
TspRI CASTG 1 cut(s) 379
XapI RAATTY 2 cut(s) 18, 760
XceI RCATGY 1 cut(s) 211
XhoI CTCGAG 1 cut(s) 146
XmiI GTMKAC 1 cut(s) 255
XspI CTAG 3 cut(s) 119, 177, 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.