Rh1DG389300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0018081
Physical Location & Seq
Reverse (-)
60815589 .. 60816786
1198 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG389300.1

Sequence Viewer

Length: 792 bp
ATGAGGAACAAGAGGTCCTCAATTTTGCCGAAGAAACTCAATGAACTCGACCACCTAGTCAACAAGACCTTCTCTGTTCAACCCGATAACCCCAGTCTCCAACGCAATGAAGCAGAAATTGAGCAGAGTCTGACGTTCGTGAAGAATCTGCTGTCGGCGGAGATGGCATCACATCCGCCGAAGCAGCACTACTTGGAAAAAATGTCGGAGAGAGTTCGAGAAATCGAGGCGGCGTTTCGCGGCTTTGGGACAGGTTCCAGTACGACAACCTCGACGCTCGACCATGTCGATAATGTTGGAGACGACGACGACGACGACGACGACGATCATGCATCGAAGTGTTCTTGCAACAAGTCTCCTCTCCAGGACGAGGAGGAAGGTGACAGTGTTTCAGATGATCTTGAGTCAACGCTTGGTCAAGATACGGGAGGTTGTGTGGATTTGGTTGAAGAGAAGAAGGCTATGCCGGTGACGGTGTTTAATAATGACTTATTTGAGGAAAAGGTTGATGATGATCGGGTTGAAGATGAGTTTCTTGATCGGATCGAAGATGTGTTTGATGATATGTCTCATAATGGCACTTCTGTTGAAGATGGGAAGGAGGAGAAGGAGATTGGGGCTGACATGGATTTGAAGGAGAGTGAAAAGAAGGAAAATGGTTTAGGGTTTGGGAAGTTATGTGGGGCTGTGGTGAGTGGGTTTGTGATTGGAATGTTTTCGATGGCTTTGATGATGGAGAGCCTTGATTACGAGGGCTATGAGGGACATGGACACTTTCTTACTCCTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

263

Amino Acids

29.24

Weight (kDa)

4.33

Isoelectric Point (pI)

52.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7610 PF24583 9 - 80 4.7e-20 Domain of unknown function (DUF7610)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018081)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g27590
malus_domestica MD07G1254300.v1.1
prunus_persica Prupe.2G277600_v2.0.a1
pyrus_communis pycom01g19370
rosa_laevigata RLG00000026736
rosa_roxburghii Rroxscaffold_4G00283470
rosa_rugosa Rorug01G0383200
rosa_samantha Rh1AG394100 Rh1BG358700 Rh1CG371700 Rh1DG389300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 56
AccII CGCG 1 cut(s) 240
AciI CCGC 4 cut(s) 158, 176, 230, 240
AclWI GGATC 1 cut(s) 551
AfaI GTAC 1 cut(s) 262
AfiI CCNNNNNNNGG 1 cut(s) 370
AgsI TTSAA 5 cut(s) 80, 449, 524, 590, 634
AjnI CCWGG 1 cut(s) 363
AloI GAACNNNNNNTCC 1 cut(s) 31
Alw26I GTCTC 4 cut(s) 101, 294, 360, 573
AlwI GGATC 1 cut(s) 551
AlwNI CAGNNNCTG 1 cut(s) 130
ApeKI GCWGC 1 cut(s) 184
Asp700I GAANNNNTTC 1 cut(s) 715
AspS9I GGNCC 1 cut(s) 15
AsuHPI GGTGA 3 cut(s) 392, 481, 703
AvaII GGWCC 1 cut(s) 15
BarI GAAGNNNNNNTAC 2 cut(s) 173, 205
BbvI GCAGC 1 cut(s) 196
BccI CCATC 4 cut(s) 157, 587, 715, 727
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BciT130I CCWGG 1 cut(s) 365
BcoDI GTCTC 4 cut(s) 101, 294, 360, 573
BfaI CTAG 1 cut(s) 56
BisI GCNGC 3 cut(s) 185, 231, 241
BlsI GCNGC 3 cut(s) 186, 232, 242
Bme1390I CCNGG 1 cut(s) 365
Bme18I GGWCC 1 cut(s) 15
BmgT120I GGNCC 1 cut(s) 15
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 1 cut(s) 365
BmrI ACTGGG 1 cut(s) 87
BmsI GCATC 2 cut(s) 176, 341
BmuI ACTGGG 1 cut(s) 87
BpmI CTGGAG 1 cut(s) 347
BpuEI CTTGAG 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 513
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 1 cut(s) 370
Bse118I RCCGGY 1 cut(s) 466
Bse1I ACTGG 2 cut(s) 93, 258
Bse3DI GCAATG 1 cut(s) 112
Bse8I GATNNNNATC 1 cut(s) 513
BseBI CCWGG 1 cut(s) 365
BseGI GGATG 1 cut(s) 172
BseJI GATNNNNATC 1 cut(s) 513
BseLI CCNNNNNNNGG 1 cut(s) 370
BseMI GCAATG 1 cut(s) 112
BseNI ACTGG 2 cut(s) 93, 258
BseRI GAGGAG 3 cut(s) 348, 386, 617
BseXI GCAGC 1 cut(s) 196
Bsh1236I CGCG 1 cut(s) 240
BsiSI CCGG 1 cut(s) 467
BslFI GGGAC 2 cut(s) 262, 777
BslI CCNNNNNNNGG 1 cut(s) 370
BsmAI GTCTC 4 cut(s) 101, 294, 360, 573
BsmBI CGTCTC 1 cut(s) 294
BsmFI GGGAC 2 cut(s) 262, 777
Bsp143I GATC 5 cut(s) 325, 397, 514, 538, 543
BspACI CCGC 4 cut(s) 158, 176, 230, 240
BspFNI CGCG 1 cut(s) 240
BspLI GGNNCC 1 cut(s) 256
BspPI GGATC 1 cut(s) 551
BsrDI GCAATG 1 cut(s) 112
BsrFI RCCGGY 1 cut(s) 466
BsrI ACTGG 2 cut(s) 93, 258
BssAI RCCGGY 1 cut(s) 466
BssMI GATC 5 cut(s) 325, 397, 514, 538, 543
Bst2UI CCWGG 1 cut(s) 365
Bst4CI ACNGT 2 cut(s) 386, 475
Bst6I CTCTTC 1 cut(s) 444
BstDEI CTNAG 1 cut(s) 789
BstF5I GGATG 1 cut(s) 172
BstFNI CGCG 1 cut(s) 240
BstKTI GATC 5 cut(s) 328, 400, 517, 541, 546
BstMAI GTCTC 4 cut(s) 101, 294, 360, 573
BstMBI GATC 5 cut(s) 325, 397, 514, 538, 543
BstMWI GCNNNNNNNGC 2 cut(s) 164, 184
BstNI CCWGG 1 cut(s) 365
BstSCI CCNGG 1 cut(s) 363
BstUI CGCG 1 cut(s) 240
BstV1I GCAGC 1 cut(s) 196
BtsCI GGATG 1 cut(s) 172
BtsIMutI CAGTG 1 cut(s) 391
CaiI CAGNNNCTG 1 cut(s) 130
Cfr10I RCCGGY 1 cut(s) 466
Cfr13I GGNCC 1 cut(s) 15
CseI GACGC 1 cut(s) 283
Csp6I GTAC 1 cut(s) 261
CviAII CATG 4 cut(s) 284, 329, 625, 767
CviJI RGCY 7 cut(s) 243, 461, 620, 686, 725, 741, 756
CviKI_1 RGCY 7 cut(s) 243, 461, 620, 686, 725, 741, 756
CviQI GTAC 1 cut(s) 261
DdeI CTNAG 1 cut(s) 789
DpnI GATC 5 cut(s) 327, 399, 516, 540, 545
DpnII GATC 5 cut(s) 325, 397, 514, 538, 543
DrdI GACNNNNNNGTC 1 cut(s) 56
DseDI GACNNNNNNGTC 1 cut(s) 56
Eam1104I CTCTTC 1 cut(s) 444
EarI CTCTTC 1 cut(s) 444
EciI GGCGGA 2 cut(s) 165, 173
Eco47I GGWCC 1 cut(s) 15
EcoO109I RGGNCCY 1 cut(s) 15
EcoRII CCWGG 1 cut(s) 363
EcoT22I ATGCAT 1 cut(s) 334
Esp3I CGTCTC 1 cut(s) 294
FaeI CATG 4 cut(s) 287, 332, 628, 770
FaiI YATR 9 cut(s) 285, 330, 464, 566, 573, 626, 679, 759, 768
FaqI GGGAC 2 cut(s) 262, 777
FatI CATG 4 cut(s) 283, 328, 624, 766
Fnu4HI GCNGC 3 cut(s) 185, 231, 241
FokI GGATG 1 cut(s) 159
Fsp4HI GCNGC 3 cut(s) 185, 231, 241
FspBI CTAG 1 cut(s) 56
GluI GCNGC 3 cut(s) 185, 231, 241
GsuI CTGGAG 1 cut(s) 347
HapII CCGG 1 cut(s) 467
HgaI GACGC 1 cut(s) 283
Hin1II CATG 4 cut(s) 287, 332, 628, 770
HincII GTYRAC 2 cut(s) 61, 408
HindII GTYRAC 2 cut(s) 61, 408
HinfI GANTC 3 cut(s) 127, 145, 404
HpaII CCGG 1 cut(s) 467
HphI GGTGA 3 cut(s) 392, 481, 703
Hpy166II GTNNAC 2 cut(s) 61, 408
Hpy188I TCNGA 4 cut(s) 132, 208, 394, 543
Hpy188III TCNNGA 5 cut(s) 139, 218, 401, 419, 536
Hpy8I GTNNAC 2 cut(s) 61, 408
Hpy99I CGWCG 8 cut(s) 277, 308, 311, 314, 317, 320, 323, 326
HpyAV CCTTC 7 cut(s) 79, 371, 451, 592, 601, 628, 643
HpyCH4III ACNGT 2 cut(s) 386, 475
HpyCH4IV ACGT 1 cut(s) 134
HpyCH4V TGCA 2 cut(s) 332, 348
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 184
HpyF3I CTNAG 1 cut(s) 789
HpySE526I ACGT 1 cut(s) 134
Hsp92II CATG 4 cut(s) 287, 332, 628, 770
Kzo9I GATC 5 cut(s) 325, 397, 514, 538, 543
LpnPI CCDG 6 cut(s) 106, 237, 271, 350, 377, 480
Lsp1109I GCAGC 1 cut(s) 196
LweI GCATC 2 cut(s) 176, 341
MaeI CTAG 1 cut(s) 56
MaeII ACGT 1 cut(s) 134
MaeIII GTNAC 2 cut(s) 380, 469
MalI GATC 5 cut(s) 327, 399, 516, 540, 545
MboI GATC 5 cut(s) 325, 397, 514, 538, 543
MboII GAAGA 7 cut(s) 43, 154, 461, 466, 536, 560, 602
MluCI AATT 2 cut(s) 21, 117
MlyI GAGTC 2 cut(s) 136, 413
MmeI TCCRAC 3 cut(s) 124, 186, 277
Mph1103I ATGCAT 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 715
MseI TTAA 1 cut(s) 480
MslI CAYNNNNRTG 1 cut(s) 337
MspI CCGG 1 cut(s) 467
MspR9I CCNGG 1 cut(s) 365
MvaI CCWGG 1 cut(s) 365
MvnI CGCG 1 cut(s) 240
MwoI GCNNNNNNNGC 2 cut(s) 164, 184
NdeII GATC 5 cut(s) 325, 397, 514, 538, 543
NlaIII CATG 4 cut(s) 287, 332, 628, 770
NlaIV GGNNCC 1 cut(s) 256
NmuCI GTSAC 2 cut(s) 380, 469
NsiI ATGCAT 1 cut(s) 334
PcsI WCGNNNNNNNCGW 7 cut(s) 269, 285, 309, 312, 315, 318, 321
PdmI GAANNNNTTC 1 cut(s) 715
PfeI GAWTC 1 cut(s) 145
PflFI GACNNNGTC 1 cut(s) 284
PfoI TCCNGGA 1 cut(s) 363
PkrI GCNGC 3 cut(s) 186, 232, 242
PleI GAGTC 2 cut(s) 135, 412
PpsI GAGTC 2 cut(s) 135, 412
PpuMI RGGWCCY 1 cut(s) 15
Psp5II RGGWCCY 1 cut(s) 15
Psp6I CCWGG 1 cut(s) 363
PspGI CCWGG 1 cut(s) 363
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 1 cut(s) 15
PspPPI RGGWCCY 1 cut(s) 15
PstNI CAGNNNCTG 1 cut(s) 130
PsyI GACNNNGTC 1 cut(s) 284
RsaI GTAC 1 cut(s) 262
RsaNI GTAC 1 cut(s) 261
RseI CAYNNNNRTG 1 cut(s) 337
SaqAI TTAA 1 cut(s) 480
SatI GCNGC 3 cut(s) 185, 231, 241
Sau3AI GATC 5 cut(s) 325, 397, 514, 538, 543
Sau96I GGNCC 1 cut(s) 15
SchI GAGTC 2 cut(s) 136, 413
ScrFI CCNGG 1 cut(s) 365
SetI ASST 9 cut(s) 17, 57, 71, 137, 256, 272, 382, 433, 507
SfaNI GCATC 2 cut(s) 176, 341
SinI GGWCC 1 cut(s) 15
SmiMI CAYNNNNRTG 1 cut(s) 337
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
Sse9I AATT 2 cut(s) 21, 117
SsiI CCGC 4 cut(s) 158, 176, 230, 240
SspMI CTAG 1 cut(s) 56
StyD4I CCNGG 1 cut(s) 363
TaaI ACNGT 2 cut(s) 386, 475
TaiI ACGT 1 cut(s) 137
TaqI TCGA 9 cut(s) 48, 217, 225, 272, 279, 288, 335, 546, 719
TasI AATT 2 cut(s) 21, 117
TauI GCSGC 2 cut(s) 233, 243
TfiI GAWTC 1 cut(s) 145
Tru1I TTAA 1 cut(s) 480
Tru9I TTAA 1 cut(s) 480
TscAI CASTG 1 cut(s) 391
TseFI GTSAC 2 cut(s) 380, 469
TseI GCWGC 1 cut(s) 184
Tsp45I GTSAC 2 cut(s) 380, 469
TspDTI ATGAA 2 cut(s) 57, 123
TspRI CASTG 1 cut(s) 391
Tth111I GACNNNGTC 1 cut(s) 284
VpaK11BI GGWCC 1 cut(s) 15
XmnI GAANNNNTTC 1 cut(s) 715
XspI CTAG 1 cut(s) 56
Zsp2I ATGCAT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.