Rroxscaffold_4G00283470

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0018081
Physical Location & Seq
Forward (+)
5414427 .. 5415215
789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00283470.1

Sequence Viewer

Length: 789 bp
ATGAGGAACAAGAGGTCCTCAATTTTGCCGAAGAAACTCAATGAACTCGACCACCTAGTCAACAAGACCTTCTCTGTTCAACCCGATAACCCCAGTCTCCAACGCAATGAAGCAGAAATTGAGCAGAGTCTGACGTTCGTGAAGAATCTGCTGTCGGCGGAGAAGGCATCACATCCGGCGAAGCAGCAGTACTACTTGGAGAAAATGTCGGAGAGAGTTCGAGAAATCGAGGCGGCGTTTCGCGGCTTTGGGACAGGTTCCGGTACGACAACCTCGACGCTCGACCATGTCGATAATGTTGGAGATGACGACGACGACGACGATCATGCATCGAAGTGTTCTTGCAACAAGTCTCCTCTCCAGGACGAGGAGGAAGGTGACAGTGTTTCAGATGATCTTGAGTCAACGCTTGGTCAAGATACGGGAGGTTGTGTGGATTGGGTTGAAGAGAAGAAGGCTATGCCGGTGACGGTGTTTAATAATGACTTATTTGAGGAAAAGGTTGATGATGATCGGGTTGAAGATGAGTTTCTTGATCGGGTCGAAGATGTGTTTGATGATATGTCTCATAATGGCGCTTCTGTTGAAGATGGGAAGGAGGAGAAGGAGATTGGGGCTGACATGGATTTGAAGGAGAGTGAAAAGAAGGAAAATGGTTTAGGGTTTGGGAAGTTATGTGGAGCTGTGGTGAGTGGGTTTGTGGTTGGAATGTTTTCGATGGCTTTGATGATGGAGAGCCTTGATTACGAGGGCTATGAGGGTCATGGACACTTTCTTACTCCTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

262

Amino Acids

29.12

Weight (kDa)

4.37

Isoelectric Point (pI)

47.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7610 PF24583 9 - 81 7.2e-19 Domain of unknown function (DUF7610)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018081)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g27590
malus_domestica MD07G1254300.v1.1
prunus_persica Prupe.2G277600_v2.0.a1
pyrus_communis pycom01g19370
rosa_laevigata RLG00000026736
rosa_roxburghii Rroxscaffold_4G00283470
rosa_rugosa Rorug01G0383200
rosa_samantha Rh1AG394100 Rh1BG358700 Rh1CG371700 Rh1DG389300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 56
AccII CGCG 1 cut(s) 243
AciI CCGC 3 cut(s) 158, 233, 243
AfaI GTAC 2 cut(s) 191, 265
AfiI CCNNNNNNNGG 1 cut(s) 367
AgsI TTSAA 5 cut(s) 80, 446, 521, 587, 631
AjnI CCWGG 1 cut(s) 360
AloI GAACNNNNNNTCC 1 cut(s) 31
AluBI AGCT 1 cut(s) 683
AluI AGCT 1 cut(s) 683
Alw26I GTCTC 3 cut(s) 101, 357, 570
AlwNI CAGNNNCTG 1 cut(s) 130
ApeKI GCWGC 1 cut(s) 184
Asp700I GAANNNNTTC 1 cut(s) 712
AspLEI GCGC 1 cut(s) 578
AspS9I GGNCC 1 cut(s) 15
AsuHPI GGTGA 3 cut(s) 389, 478, 700
AvaII GGWCC 1 cut(s) 15
BarI GAAGNNNNNNTAC 2 cut(s) 173, 205
BbvI GCAGC 1 cut(s) 196
BccI CCATC 3 cut(s) 584, 712, 724
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciT130I CCWGG 1 cut(s) 362
BcoDI GTCTC 3 cut(s) 101, 357, 570
BfaI CTAG 1 cut(s) 56
BfoI RGCGCY 1 cut(s) 579
BisI GCNGC 3 cut(s) 185, 234, 244
BlsI GCNGC 3 cut(s) 186, 235, 245
BmcAI AGTACT 1 cut(s) 191
Bme1390I CCNGG 1 cut(s) 362
Bme18I GGWCC 1 cut(s) 15
BmgT120I GGNCC 1 cut(s) 15
BmiI GGNNCC 1 cut(s) 259
BmrFI CCNGG 1 cut(s) 362
BmrI ACTGGG 1 cut(s) 87
BmsI GCATC 2 cut(s) 176, 338
BmuI ACTGGG 1 cut(s) 87
BpmI CTGGAG 1 cut(s) 344
BpuEI CTTGAG 1 cut(s) 419
BsaBI GATNNNNATC 1 cut(s) 510
BsaWI WCCGGW 1 cut(s) 260
BsaXI ACNNNNNCTCC 6 cut(s) 191, 221, 417, 447, 672, 702
Bsc4I CCNNNNNNNGG 1 cut(s) 367
Bse118I RCCGGY 1 cut(s) 463
Bse1I ACTGG 1 cut(s) 93
Bse3DI GCAATG 1 cut(s) 112
Bse8I GATNNNNATC 1 cut(s) 510
BseBI CCWGG 1 cut(s) 362
BseGI GGATG 1 cut(s) 172
BseJI GATNNNNATC 1 cut(s) 510
BseLI CCNNNNNNNGG 1 cut(s) 367
BseMI GCAATG 1 cut(s) 112
BseNI ACTGG 1 cut(s) 93
BseRI GAGGAG 3 cut(s) 345, 383, 614
BseXI GCAGC 1 cut(s) 196
Bsh1236I CGCG 1 cut(s) 243
BsiSI CCGG 3 cut(s) 176, 261, 464
BslFI GGGAC 1 cut(s) 265
BslI CCNNNNNNNGG 1 cut(s) 367
BsmAI GTCTC 3 cut(s) 101, 357, 570
BsmFI GGGAC 1 cut(s) 265
Bsp143I GATC 4 cut(s) 322, 394, 511, 535
BspACI CCGC 3 cut(s) 158, 233, 243
BspFNI CGCG 1 cut(s) 243
BspLI GGNNCC 1 cut(s) 259
BsrDI GCAATG 1 cut(s) 112
BsrFI RCCGGY 1 cut(s) 463
BsrI ACTGG 1 cut(s) 93
BssAI RCCGGY 1 cut(s) 463
BssMI GATC 4 cut(s) 322, 394, 511, 535
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 2 cut(s) 383, 472
Bst6I CTCTTC 1 cut(s) 441
BstDEI CTNAG 1 cut(s) 786
BstF5I GGATG 1 cut(s) 172
BstFNI CGCG 1 cut(s) 243
BstH2I RGCGCY 1 cut(s) 579
BstHHI GCGC 1 cut(s) 578
BstKTI GATC 4 cut(s) 325, 397, 514, 538
BstMAI GTCTC 3 cut(s) 101, 357, 570
BstMBI GATC 4 cut(s) 322, 394, 511, 535
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNI CCWGG 1 cut(s) 362
BstSCI CCNGG 1 cut(s) 360
BstUI CGCG 1 cut(s) 243
BstV1I GCAGC 1 cut(s) 196
BtsCI GGATG 1 cut(s) 172
BtsIMutI CAGTG 1 cut(s) 388
CaiI CAGNNNCTG 1 cut(s) 130
CfoI GCGC 1 cut(s) 578
Cfr10I RCCGGY 1 cut(s) 463
Cfr13I GGNCC 1 cut(s) 15
CseI GACGC 1 cut(s) 286
Csp6I GTAC 2 cut(s) 190, 264
CviAII CATG 4 cut(s) 287, 326, 622, 764
CviJI RGCY 7 cut(s) 246, 458, 617, 683, 722, 738, 753
CviKI_1 RGCY 7 cut(s) 246, 458, 617, 683, 722, 738, 753
CviQI GTAC 2 cut(s) 190, 264
DdeI CTNAG 1 cut(s) 786
DpnI GATC 4 cut(s) 324, 396, 513, 537
DpnII GATC 4 cut(s) 322, 394, 511, 535
DrdI GACNNNNNNGTC 1 cut(s) 56
DseDI GACNNNNNNGTC 1 cut(s) 56
Eam1104I CTCTTC 1 cut(s) 441
EarI CTCTTC 1 cut(s) 441
EciI GGCGGA 1 cut(s) 173
Eco47I GGWCC 1 cut(s) 15
EcoO109I RGGNCCY 1 cut(s) 15
EcoRII CCWGG 1 cut(s) 360
EcoT22I ATGCAT 1 cut(s) 331
FaeI CATG 4 cut(s) 290, 329, 625, 767
FaiI YATR 9 cut(s) 288, 327, 461, 563, 570, 623, 676, 756, 765
FaqI GGGAC 1 cut(s) 265
FatI CATG 4 cut(s) 286, 325, 621, 763
Fnu4HI GCNGC 3 cut(s) 185, 234, 244
FokI GGATG 1 cut(s) 159
Fsp4HI GCNGC 3 cut(s) 185, 234, 244
FspBI CTAG 1 cut(s) 56
GlaI GCGC 1 cut(s) 577
GluI GCNGC 3 cut(s) 185, 234, 244
GsuI CTGGAG 1 cut(s) 344
HaeII RGCGCY 1 cut(s) 579
HapII CCGG 3 cut(s) 176, 261, 464
HgaI GACGC 1 cut(s) 286
HhaI GCGC 1 cut(s) 578
Hin1II CATG 4 cut(s) 290, 329, 625, 767
Hin6I GCGC 1 cut(s) 576
HinP1I GCGC 1 cut(s) 576
HincII GTYRAC 2 cut(s) 61, 405
HindII GTYRAC 2 cut(s) 61, 405
HinfI GANTC 3 cut(s) 127, 145, 401
HpaII CCGG 3 cut(s) 176, 261, 464
HphI GGTGA 3 cut(s) 389, 478, 700
Hpy166II GTNNAC 2 cut(s) 61, 405
Hpy188I TCNGA 3 cut(s) 132, 211, 391
Hpy188III TCNNGA 5 cut(s) 139, 221, 398, 416, 533
Hpy8I GTNNAC 2 cut(s) 61, 405
Hpy99I CGWCG 5 cut(s) 280, 314, 317, 320, 323
HpyAV CCTTC 8 cut(s) 79, 157, 368, 448, 589, 598, 625, 640
HpyCH4III ACNGT 2 cut(s) 383, 472
HpyCH4IV ACGT 1 cut(s) 134
HpyCH4V TGCA 2 cut(s) 329, 345
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 1 cut(s) 786
HpySE526I ACGT 1 cut(s) 134
Hsp92II CATG 4 cut(s) 290, 329, 625, 767
HspAI GCGC 1 cut(s) 576
Kzo9I GATC 4 cut(s) 322, 394, 511, 535
LmnI GCTCC 1 cut(s) 680
LpnPI CCDG 7 cut(s) 106, 189, 240, 274, 347, 374, 477
Lsp1109I GCAGC 1 cut(s) 196
LweI GCATC 2 cut(s) 176, 338
MaeI CTAG 1 cut(s) 56
MaeII ACGT 1 cut(s) 134
MaeIII GTNAC 2 cut(s) 377, 466
MalI GATC 4 cut(s) 324, 396, 513, 537
MboI GATC 4 cut(s) 322, 394, 511, 535
MboII GAAGA 7 cut(s) 43, 154, 458, 463, 533, 557, 599
MluCI AATT 2 cut(s) 21, 117
MlyI GAGTC 2 cut(s) 136, 410
MmeI TCCRAC 4 cut(s) 124, 189, 280, 685
Mph1103I ATGCAT 1 cut(s) 331
MroXI GAANNNNTTC 1 cut(s) 712
MseI TTAA 1 cut(s) 477
MslI CAYNNNNRTG 1 cut(s) 334
MspI CCGG 3 cut(s) 176, 261, 464
MspR9I CCNGG 1 cut(s) 362
MvaI CCWGG 1 cut(s) 362
MvnI CGCG 1 cut(s) 243
MwoI GCNNNNNNNGC 1 cut(s) 164
NdeII GATC 4 cut(s) 322, 394, 511, 535
NlaIII CATG 4 cut(s) 290, 329, 625, 767
NlaIV GGNNCC 1 cut(s) 259
NmuCI GTSAC 2 cut(s) 377, 466
NsiI ATGCAT 1 cut(s) 331
PcsI WCGNNNNNNNCGW 4 cut(s) 272, 288, 315, 318
PdmI GAANNNNTTC 1 cut(s) 712
PfeI GAWTC 1 cut(s) 145
PflFI GACNNNGTC 1 cut(s) 287
PfoI TCCNGGA 1 cut(s) 360
PkrI GCNGC 3 cut(s) 186, 235, 245
PleI GAGTC 2 cut(s) 135, 409
PpsI GAGTC 2 cut(s) 135, 409
PpuMI RGGWCCY 1 cut(s) 15
Psp5II RGGWCCY 1 cut(s) 15
Psp6I CCWGG 1 cut(s) 360
PspGI CCWGG 1 cut(s) 360
PspN4I GGNNCC 1 cut(s) 259
PspPI GGNCC 1 cut(s) 15
PspPPI RGGWCCY 1 cut(s) 15
PstNI CAGNNNCTG 1 cut(s) 130
PsyI GACNNNGTC 1 cut(s) 287
RsaI GTAC 2 cut(s) 191, 265
RsaNI GTAC 2 cut(s) 190, 264
RseI CAYNNNNRTG 1 cut(s) 334
SaqAI TTAA 1 cut(s) 477
SatI GCNGC 3 cut(s) 185, 234, 244
Sau3AI GATC 4 cut(s) 322, 394, 511, 535
Sau96I GGNCC 1 cut(s) 15
ScaI AGTACT 1 cut(s) 191
SchI GAGTC 2 cut(s) 136, 410
ScrFI CCNGG 1 cut(s) 362
SfaNI GCATC 2 cut(s) 176, 338
SinI GGWCC 1 cut(s) 15
SmiMI CAYNNNNRTG 1 cut(s) 334
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 2 cut(s) 21, 117
SsiI CCGC 3 cut(s) 158, 233, 243
SspMI CTAG 1 cut(s) 56
StyD4I CCNGG 1 cut(s) 360
TaaI ACNGT 2 cut(s) 383, 472
TaiI ACGT 1 cut(s) 137
TaqI TCGA 9 cut(s) 48, 220, 228, 275, 282, 291, 332, 543, 716
TasI AATT 2 cut(s) 21, 117
TatI WGTACW 1 cut(s) 189
TauI GCSGC 2 cut(s) 236, 246
TfiI GAWTC 1 cut(s) 145
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TscAI CASTG 1 cut(s) 388
TseFI GTSAC 2 cut(s) 377, 466
TseI GCWGC 1 cut(s) 184
Tsp45I GTSAC 2 cut(s) 377, 466
TspDTI ATGAA 2 cut(s) 57, 123
TspRI CASTG 1 cut(s) 388
Tth111I GACNNNGTC 1 cut(s) 287
VpaK11BI GGWCC 1 cut(s) 15
XmnI GAANNNNTTC 1 cut(s) 712
XspI CTAG 1 cut(s) 56
ZrmI AGTACT 1 cut(s) 191
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.