FvH4_7g28070

receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0012362
Physical Location & Seq
Forward (+)
20856673 .. 20861681
5009 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g28070.t1

Sequence Viewer

Length: 3189 bp
ATGGCCACCACAGTTTTCCTTTTCACCTTGCTCTTGTTCTTCTTCACAAACCCACCTCATGTTTATGGAAACACAGAGCTGAGAGCACTCATGGACATCAAGGCCCTTTTGGACCCCCAGAGTCTGTACCTTTCTTCATGGACTGTCCATGGAGACCCCTGCGACACGTCGTTTGAAGGCGTAGCGTGCAATGAAGCGGGTCGGGTTTCGAATATTTCATTGCAAGGGAAAGGGCTAGATGGGAAGCTGTCTCCTGCCATTGGAGAGCTCAAGGATTTGACTGGGATTTACATGCATTATAACTCCTTGAAGGGAGAGATACCCAAAGAGATTGCCAACTTGACTCAGCTCAGTGACTTGTATTTGAATGTTAATAATCTAAGTGGAGAGATTCCTCCTGAGATTGGAAACATGGGCAGCTTGGAAGTTTTGCAGCTTTGTTACAATCAGCTAACTGGAAGCATACCAACGCAGCTGGGGTCTTTAAAGAACCTCAGTGTTCTTGCTCTGCAGTCCAACCAGTTAACCGGTGCGATTCCAGCAAGTTTAGGAAACTTGGAAATGCTAATGAGACTCGATCTGAGCTACAACAACCTCTTTGGTTCTATTCCCATGAAAGTAGCTGATGCCCCTTTGCTTGAAGTTCTGGACATTCGGAATAATACCCTCTCTGGCAATGTACCTCTTGCTCTGAAGAGACTAAATGACGGATTCTCTTATGAAAACAATCTGGGATTGTGTGGAGCCGGATTTATGTCCTTAACAGCCTGTAACACTTCCAGTCATCTTAATGCAAGTAGACCTGAACCCTTTGGATCGACTGGGACAACACGAGCTATCCCGGAGACTGCAAATGTGCAGTTGCCTTGCAACCAGTCTCGGTGCTCGAATATGTCAAAATCCCATCAAGCATCTGTTGCTGTTGTTGTTGGTATTGTCGTACTAACAGTTGCCTTATCGGCTATAGGAATTCTACTTTTCACACAGCATCGTCGTCAAAAACAGAAGCTTGGGTCCTCATTTGATGTTTCTGATGCCCGTCTTAGTGTTGATGAGACTAAGGCTAAGGGAGTAGCTCACAGGAAATATGGCTCTCCACTTATGAGCCTTGAGTATTCTCAAGGGTGGGATCCTTTAGCTGATGGGAGGAATTTAAGTGCATTTGCCCAAGACGTGTTCCATAGCTTTAAGTTCAATTTGGATGAGGTGGAGACTGCCACTCAGTATTTCTCTGAGGTGAATCTATTGGGGAAGAGTAACTTCTCTGCAACCTATAAAGGAATATTACGAGATGGGACTGTTGTGGCTGTTAAGAGCATTGGAAAAACTTGCTGCAAGACTGAGGAAGCAGAGTTCTTGAAGGGGTTGAACATGTTAACCTCATTGAGGCATGAAAATTTAGTGAAATTGAGGGGATTTTGTTGTTCAAGGGGCCGCGGAGAGTGCTTTCTCATTTATGAATATGTTCCAAATGGAAGTTTGCTGCGATATCTTGATGTGAAGGAGGGCGACAGCCATGTTCTTGAATGGTCAACTAGAGTTTCTATTGTGAAGGGCATCGCAAAAGGTCTATCTTATCTACATGGATACAAGCCAAACAAACCGGCTCTTGTTCACCAAAACATTTCAGCTGAAAAAGTGCTGATTGACCAGCGATTCAACCCTTTGCTTTCAAATTCCGGCCTGCACAAGCTTCTTACCAATGATGTCGTTTTCTCAGCACTCAAGGCCAGCGCTGCTATGGGCTACCTAGCTCCAGAGTACACCACTACCGGACGGTTTACAGAGAAAAGTGATGTCTATGCATTTGGAGTGCTGGTTTTCCAAATCCTATCAGGGAAACGGAAAGTAACTAACTCCATGCGTCTTGGTGCTGAGTCAGGCAGATTTGAAGACTATATTGACCAAAACCTCAATGGCAGGTTCTTTGAATATGAAGCGTCCAAGCTTGCAAACACTGCTTTGCTCTGCACTCATGAATCTCCAATTGAGAGACCATCTATTGGAGAAGTTGTTCAAGAGTTAATGCCCCCCAGTGACAACGATGATGACTTTGAAAAATGGCAGAAACAGATTACTAAGGCAGAAGCATATAGTGAAAGACCTTCCACACCACCAGAGGGCGAGGAAGAGTTCACTGACGATGATGGCACTCATTATAAGTGGGATTGCGGTCTTGGGGCTTGGGTTCCTCAGGATAGCATGTTTGGGGTAGAGGATATGACTTTCATGCAAGAAGAGGAAGTCTTCACGAATGTGAAACTTTCTAATGGTTCTTCAACTGGAAATTTTTCTAATCCTCCTAAAGAGAAAGACATTAATGTTAACCGTGAGGTAGAAGAAGGGAATTCAGTCGAAGAAAAGAATTCAACTTCAAAGAGAAAACTATCGGATATGGAAGATATCAAAAAGGAAGCTAATAAACCTCCTGATAGTTGGTTTGAGCTAAAGGTCAATTCACACGTATATGTGACTGGATTACCTGATGATGTTACAGTTGGCGAAGTGGTAGAAGTGTTTTCTAAGTGTGGGATAATAAAAGAGGATCCTGAATTAAAAAAGCCTCGTGTGAAGTTGTATACTGATAAAGCGACGGGAAAGATAAAAGGAGATGCACTTGTTACATATCTAAAGGAACCCTCAGTCGATTTGGCTCTCAAAATTTTTGACGGAACACCTCTGCGTTTGGGCGGCAAGGTTTTCATGTCAGTCAACCGAGCTAAGTTTGAGCAGAAAGGGGATAGCTTTATTGCGAAACAAGCAGACAACAAGAGGAAAAAAAAACTGAAGAAGGTGGAAGAGAAGATGCTTGGTTGGGGTGGTCGTGATGATGCAAAACTGTTGATTCCAGCTACTGTTCTTCTTTGTTATATGTTCACTATTGCTGAAATGAGGGCAGATGAAAACTTACGTAGAGAGTTGCAGGTAGATGTTGAAGAGGAATGTCAGAAGCTTGGTCCAGTGGACTACGTCAAGGTTTGTGAGAATCATCTTCAGGGTGTTGTTTTGGTAAGATTCAAGCATAGGAAGGATGCTCAAAAGTGCATAGAGTTGATGAACGGAAGATGGTTTGGTGGCAGGCAGATACATGCAAGTGAGGATAATGGTTTAATTAATCATGCTTTGGTCAGGAATCTGGATGATGATGCAGAACGTTTAGAGTTGTTTGCTTCCGAACTTGATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase_Tyr

Protein Analysis

1063

Amino Acids

117.73

Weight (kDa)

5.72

Isoelectric Point (pI)

31.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 64 1.6e-07 Leucine rich repeat N-terminal domain
LRR_14 PF23598 81 - 220 2.9e-11 Leucine-rich repeat region
LRR_8 PF13855 140 - 199 7.3e-08 Leucine rich repeat
Pkinase PF00069 411 - 673 1.7e-24 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 414 - 675 6e-38 Protein tyrosine and serine/threonine kinase
RRM_1 PF00076 977 - 1030 4e-07 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51560
fragaria_vesca FvH4_7g28070
malus_domestica MD01G1184900.v1.1 MD07G1257200.v1.1
prunus_persica Prupe.2G281400_v2.0.a1
pyrus_communis pycom01g19660 pycom07g23010 pycom07g23020
rosa_chinensis RchiOBHm_Chr1g0375021
rosa_laevigata RLG00000026697
rosa_multiflora Rmu_sc0000554.1_g000005 Rmu_sc0017099.1_g000006
rosa_roxburghii Rroxscaffold_4G00283080
rosa_rugosa Rorug01G0387500
rosa_samantha Rh1AG398100 Rh1BG362000 Rh1CG375700 Rh1DG393200
rosa_wichuraiana Rw1G035330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 300, 2160
Acc36I ACCTGC 2 cut(s) 1911, 2914
AccB7I CCANNNNNTGG 1 cut(s) 2003
AccI GTMKAC 2 cut(s) 799, 2579
AccII CGCG 1 cut(s) 1437
AciI CCGC 5 cut(s) 197, 1435, 1437, 2172, 2691
AclI AACGTT 1 cut(s) 3154
AclWI GGATC 5 cut(s) 823, 1124, 1137, 2540, 2553
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 8 cut(s) 969, 1150, 1396, 1675, 2287, 2347, 2365, 2661
AcuI CTGAAG 3 cut(s) 713, 2807, 2978
AfaI GTAC 4 cut(s) 128, 681, 942, 1763
AfeI AGCGCT 1 cut(s) 1735
AfiI CCNNNNNNNGG 5 cut(s) 260, 404, 1386, 2003, 2120
AflIII ACRYGT 4 cut(s) 165, 1173, 1371, 2461
AgeI ACCGGT 1 cut(s) 527
AjiI CACGTC 2 cut(s) 168, 1174
AleI CACNNNNGTG 1 cut(s) 2255
AloI GAACNNNNNNTCC 4 cut(s) 1337, 1369, 1998, 2030
Alw21I GWGCWC 3 cut(s) 88, 270, 887
Alw26I GTCTC 9 cut(s) 147, 255, 565, 691, 839, 882, 1049, 1205, 1987
AlwI GGATC 5 cut(s) 823, 1124, 1137, 2540, 2553
AlwNI CAGNNNCTG 2 cut(s) 124, 2855
Aor51HI AGCGCT 1 cut(s) 1735
AoxI GGCC 5 cut(s) 3, 102, 1432, 1681, 1728
ApeKI GCWGC 6 cut(s) 417, 433, 472, 1332, 1483, 1736
ApoI RAATTY 8 cut(s) 969, 1150, 1396, 1675, 2287, 2347, 2365, 2661
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AseI ATTAAT 2 cut(s) 2319, 3114
AsiGI ACCGGT 1 cut(s) 527
Asp700I GAANNNNTTC 3 cut(s) 1464, 2013, 2290
AspLEI GCGC 1 cut(s) 1736
AspS9I GGNCC 5 cut(s) 103, 112, 1014, 1432, 2957
AsuC2I CCSGG 1 cut(s) 842
AsuHPI GGTGA 3 cut(s) 16, 1249, 1607
AsuII TTCGAA 1 cut(s) 209
AvaII GGWCC 3 cut(s) 112, 1014, 2957
AxyI CCTNAGG 1 cut(s) 2193
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 2 cut(s) 1129, 2545
BanII GRGCYC 1 cut(s) 270
BauI CACGAG 2 cut(s) 831, 2565
BbsI GAAGAC 2 cut(s) 1899, 2239
Bbv12I GWGCWC 3 cut(s) 88, 270, 887
BbvI GCAGC 6 cut(s) 429, 445, 484, 1319, 1470, 1723
BccI CCATC 7 cut(s) 233, 912, 1136, 1286, 2005, 2141, 3060
BciVI GTATCC 1 cut(s) 1580
BcnI CCSGG 1 cut(s) 842
BcoDI GTCTC 9 cut(s) 147, 255, 565, 691, 839, 882, 1049, 1205, 1987
BfaI CTAG 3 cut(s) 236, 1536, 1751
BfmI CTRYAG 2 cut(s) 509, 963
BfoI RGCGCY 1 cut(s) 1737
BfuAI ACCTGC 2 cut(s) 1911, 2914
BfuI GTATCC 1 cut(s) 1580
BglI GCCNNNNNGGC 1 cut(s) 959
BisI GCNGC 8 cut(s) 418, 434, 473, 1333, 1435, 1484, 1737, 2692
BlsI GCNGC 8 cut(s) 419, 435, 474, 1334, 1436, 1485, 1738, 2693
Bme1390I CCNGG 1 cut(s) 842
Bme18I GGWCC 3 cut(s) 112, 1014, 2957
BmgBI CACGTC 2 cut(s) 168, 1174
BmgT120I GGNCC 5 cut(s) 103, 112, 1014, 1432, 2957
BmiI GGNNCC 8 cut(s) 114, 745, 1015, 1131, 1433, 2190, 2547, 2637
BmrFI CCNGG 1 cut(s) 842
BmrI ACTGGG 3 cut(s) 291, 831, 2028
BmuI ACTGGG 3 cut(s) 291, 831, 2028
BpiI GAAGAC 2 cut(s) 1899, 2239
BpmI CTGGAG 1 cut(s) 1740
Bpu10I CCTNAGC 1 cut(s) 1065
Bpu14I TTCGAA 1 cut(s) 209
BpuEI CTTGAG 4 cut(s) 254, 1104, 1130, 1709
BpuMI CCSGG 1 cut(s) 842
BsaAI YACGTR 2 cut(s) 2464, 2912
BsaI GGTCTC 2 cut(s) 147, 1987
BsaJI CCNNGG 2 cut(s) 148, 1435
BsaWI WCCGGW 2 cut(s) 527, 1772
BsaXI ACNNNNNCTCC 4 cut(s) 1803, 1833, 1998, 2028
Bsc4I CCNNNNNNNGG 5 cut(s) 260, 404, 1386, 2003, 2120
Bse118I RCCGGY 2 cut(s) 527, 1603
Bse21I CCTNAGG 1 cut(s) 2193
Bse3DI GCAATG 3 cut(s) 196, 218, 682
BseDI CCNNGG 2 cut(s) 148, 1435
BseGI GGATG 3 cut(s) 1207, 3037, 3145
BseLI CCNNNNNNNGG 5 cut(s) 260, 404, 1386, 2003, 2120
BseMI GCAATG 3 cut(s) 196, 218, 682
BseXI GCAGC 6 cut(s) 429, 445, 484, 1319, 1470, 1723
BseYI CCCAGC 1 cut(s) 475
BsgI GTGCAG 3 cut(s) 878, 1670, 1954
Bsh1236I CGCG 1 cut(s) 1437
BshFI GGCC 5 cut(s) 5, 104, 1434, 1683, 1730
BshTI ACCGGT 1 cut(s) 527
BsiHKAI GWGCWC 3 cut(s) 88, 270, 887
BsiSI CCGG 6 cut(s) 528, 747, 842, 1604, 1680, 1773
BslFI GGGAC 2 cut(s) 838, 1309
BslI CCNNNNNNNGG 5 cut(s) 260, 404, 1386, 2003, 2120
BsmAI GTCTC 9 cut(s) 147, 255, 565, 691, 839, 882, 1049, 1205, 1987
BsmFI GGGAC 2 cut(s) 838, 1309
BsnI GGCC 5 cut(s) 5, 104, 1434, 1683, 1730
Bso31I GGTCTC 2 cut(s) 147, 1987
Bsp119I TTCGAA 1 cut(s) 209
Bsp1286I GDGCHC 3 cut(s) 88, 270, 887
Bsp143I GATC 4 cut(s) 577, 815, 1129, 2545
Bsp19I CCATGG 1 cut(s) 148
BspACI CCGC 5 cut(s) 197, 1435, 1437, 2172, 2691
BspANI GGCC 5 cut(s) 5, 104, 1434, 1683, 1730
BspFNI CGCG 1 cut(s) 1437
BspHI TCATGA 1 cut(s) 1975
BspLI GGNNCC 8 cut(s) 114, 745, 1015, 1131, 1433, 2190, 2547, 2637
BspMAI CTGCAG 1 cut(s) 513
BspMI ACCTGC 2 cut(s) 1911, 2914
BspPI GGATC 5 cut(s) 823, 1124, 1137, 2540, 2553
BspT104I TTCGAA 1 cut(s) 209
BspTNI GGTCTC 2 cut(s) 147, 1987
BsrDI GCAATG 3 cut(s) 196, 218, 682
BsrFI RCCGGY 2 cut(s) 527, 1603
BssAI RCCGGY 2 cut(s) 527, 1603
BssECI CCNNGG 2 cut(s) 148, 1435
BssMI GATC 4 cut(s) 577, 815, 1129, 2545
BssNAI GTATAC 1 cut(s) 2580
BssSI CACGAG 2 cut(s) 831, 2565
BssT1I CCWWGG 1 cut(s) 148
Bst1107I GTATAC 1 cut(s) 2580
Bst2BI CACGAG 2 cut(s) 831, 2565
Bst4CI ACNGT 9 cut(s) 13, 145, 949, 1300, 1779, 2330, 2497, 2841, 2857
Bst6I CTCTTC 6 cut(s) 689, 1247, 2124, 2232, 2793, 2931
BstAPI GCANNNNNTGC 2 cut(s) 917, 1958
BstBAI YACGTR 2 cut(s) 2464, 2912
BstBI TTCGAA 1 cut(s) 209
BstC8I GCNNGC 5 cut(s) 187, 1685, 1732, 1950, 3080
BstDSI CCRYGG 2 cut(s) 148, 1435
BstENI CCTNNNNNAGG 1 cut(s) 1384
BstF5I GGATG 3 cut(s) 1207, 3037, 3145
BstFNI CGCG 1 cut(s) 1437
BstH2I RGCGCY 1 cut(s) 1737
BstHHI GCGC 1 cut(s) 1736
BstKTI GATC 4 cut(s) 580, 818, 1132, 2548
BstMAI GTCTC 9 cut(s) 147, 255, 565, 691, 839, 882, 1049, 1205, 1987
BstMBI GATC 4 cut(s) 577, 815, 1129, 2545
BstMWI GCNNNNNNNGC 9 cut(s) 186, 539, 917, 959, 1443, 1727, 1736, 1958, 2759
BstNSI RCATGY 4 cut(s) 295, 1375, 2206, 3092
BstSCI CCNGG 1 cut(s) 840
BstSFI CTRYAG 2 cut(s) 509, 963
BstSNI TACGTA 1 cut(s) 2912
BstUI CGCG 1 cut(s) 1437
BstV1I GCAGC 6 cut(s) 429, 445, 484, 1319, 1470, 1723
BstV2I GAAGAC 2 cut(s) 1899, 2239
BstX2I RGATCY 2 cut(s) 1129, 2545
BstYI RGATCY 2 cut(s) 1129, 2545
BstZ17I GTATAC 1 cut(s) 2580
Bsu36I CCTNAGG 1 cut(s) 2193
BsuI GTATCC 1 cut(s) 1580
BsuRI GGCC 5 cut(s) 5, 104, 1434, 1683, 1730
BtgI CCRYGG 2 cut(s) 148, 1435
BtgZI GCGATG 1 cut(s) 1543
BtrI CACGTC 2 cut(s) 168, 1174
BtsCI GGATG 3 cut(s) 1207, 3037, 3145
BtsI GCAGTG 1 cut(s) 1956
BtsIMutI CAGTG 6 cut(s) 358, 502, 1956, 2041, 2136, 2967
BveI ACCTGC 2 cut(s) 1911, 2914
Cac8I GCNNGC 5 cut(s) 187, 1685, 1732, 1950, 3080
CaiI CAGNNNCTG 2 cut(s) 124, 2855
CciI TCATGA 1 cut(s) 1975
CfoI GCGC 1 cut(s) 1736
Cfr10I RCCGGY 2 cut(s) 527, 1603
Cfr13I GGNCC 5 cut(s) 103, 112, 1014, 1432, 2957
Cfr42I CCGCGG 1 cut(s) 1438
CseI GACGC 2 cut(s) 1853, 1929
Csp6I GTAC 4 cut(s) 127, 680, 941, 1762
CspAI ACCGGT 1 cut(s) 527
CviQI GTAC 4 cut(s) 127, 680, 941, 1762
DpnI GATC 4 cut(s) 579, 817, 1131, 2547
DpnII GATC 4 cut(s) 577, 815, 1129, 2545
DraI TTTAAA 1 cut(s) 486
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 6 cut(s) 689, 1247, 2124, 2232, 2793, 2931
EarI CTCTTC 6 cut(s) 689, 1247, 2124, 2232, 2793, 2931
Ecl136II GAGCTC 1 cut(s) 268
Eco105I TACGTA 1 cut(s) 2912
Eco130I CCWWGG 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 270
Eco31I GGTCTC 2 cut(s) 147, 1987
Eco32I GATATC 2 cut(s) 1490, 2404
Eco47I GGWCC 3 cut(s) 112, 1014, 2957
Eco47III AGCGCT 1 cut(s) 1735
Eco53kI GAGCTC 1 cut(s) 268
Eco57I CTGAAG 3 cut(s) 713, 2807, 2978
Eco81I CCTNAGG 1 cut(s) 2193
EcoICRI GAGCTC 1 cut(s) 268
EcoNI CCTNNNNNAGG 1 cut(s) 1384
EcoO109I RGGNCCY 2 cut(s) 103, 1014
EcoRI GAATTC 3 cut(s) 969, 2347, 2365
EcoRV GATATC 2 cut(s) 1490, 2404
EcoT14I CCWWGG 1 cut(s) 148
EcoT22I ATGCAT 2 cut(s) 297, 1807
EcoT38I GRGCYC 1 cut(s) 270
ErhI CCWWGG 1 cut(s) 148
FalI AAGNNNNNCTT 2 cut(s) 1244, 1276
FaqI GGGAC 2 cut(s) 838, 1309
FauI CCCGC 1 cut(s) 190
FblI GTMKAC 2 cut(s) 799, 2579
Fnu4HI GCNGC 8 cut(s) 418, 434, 473, 1333, 1435, 1484, 1737, 2692
FokI GGATG 3 cut(s) 1214, 3044, 3152
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 8 cut(s) 418, 434, 473, 1333, 1435, 1484, 1737, 2692
FspBI CTAG 3 cut(s) 236, 1536, 1751
GlaI GCGC 1 cut(s) 1735
GluI GCNGC 8 cut(s) 418, 434, 473, 1333, 1435, 1484, 1737, 2692
GsaI CCCAGC 1 cut(s) 479
GsuI CTGGAG 1 cut(s) 1740
HaeII RGCGCY 1 cut(s) 1737
HaeIII GGCC 5 cut(s) 5, 104, 1434, 1683, 1730
HapII CCGG 6 cut(s) 528, 747, 842, 1604, 1680, 1773
HgaI GACGC 2 cut(s) 1853, 1929
HhaI GCGC 1 cut(s) 1736
Hin6I GCGC 1 cut(s) 1734
HinP1I GCGC 1 cut(s) 1734
HincII GTYRAC 5 cut(s) 525, 1377, 1533, 2326, 2713
HindII GTYRAC 5 cut(s) 525, 1377, 1533, 2326, 2713
HindIII AAGCTT 4 cut(s) 1007, 1691, 1946, 2951
HpaI GTTAAC 3 cut(s) 525, 1377, 2326
HpaII CCGG 6 cut(s) 528, 747, 842, 1604, 1680, 1773
HphI GGTGA 3 cut(s) 16, 1249, 1607
Hpy188I TCNGA 8 cut(s) 582, 657, 693, 1033, 1234, 2392, 2949, 3175
Hpy99I CGWCG 3 cut(s) 172, 996, 2596
HpyAV CCTTC 9 cut(s) 170, 304, 1354, 1495, 1546, 2115, 2336, 2785, 3022
HpyCH4III ACNGT 9 cut(s) 13, 145, 949, 1300, 1779, 2330, 2497, 2841, 2857
HpyCH4IV ACGT 6 cut(s) 167, 1173, 2463, 2911, 2970, 3154
HpyF10VI GCNNNNNNNGC 9 cut(s) 186, 539, 917, 959, 1443, 1727, 1736, 1958, 2759
HpySE526I ACGT 6 cut(s) 167, 1173, 2463, 2911, 2970, 3154
HspAI GCGC 1 cut(s) 1734
KspAI GTTAAC 3 cut(s) 525, 1377, 2326
KspI CCGCGG 1 cut(s) 1438
Kzo9I GATC 4 cut(s) 577, 815, 1129, 2545
LmnI GCTCC 2 cut(s) 743, 1759
Lsp1109I GCAGC 6 cut(s) 429, 445, 484, 1319, 1470, 1723
MaeI CTAG 3 cut(s) 236, 1536, 1751
MaeII ACGT 6 cut(s) 167, 1173, 2463, 2911, 2970, 3154
MaeIII GTNAC 9 cut(s) 353, 440, 770, 1256, 1849, 2036, 2470, 2491, 2620
MalI GATC 4 cut(s) 579, 817, 1131, 2547
MboI GATC 4 cut(s) 577, 815, 1129, 2545
MfeI CAATTG 1 cut(s) 1986
MflI RGATCY 2 cut(s) 1129, 2545
MhlI GDGCHC 3 cut(s) 88, 270, 887
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 130, 337, 567, 1886
MmeI TCCRAC 1 cut(s) 540
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 2 cut(s) 297, 1807
MroXI GAANNNNTTC 3 cut(s) 1464, 2013, 2290
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 5 cut(s) 63, 789, 2255, 2466, 3093
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 475, 1437, 1631
MspI CCGG 6 cut(s) 528, 747, 842, 1604, 1680, 1773
MspR9I CCNGG 1 cut(s) 842
MunI CAATTG 1 cut(s) 1986
MvnI CGCG 1 cut(s) 1437
MwoI GCNNNNNNNGC 9 cut(s) 186, 539, 917, 959, 1443, 1727, 1736, 1958, 2759
NciI CCSGG 1 cut(s) 842
NcoI CCATGG 1 cut(s) 148
NdeII GATC 4 cut(s) 577, 815, 1129, 2545
NlaIV GGNNCC 8 cut(s) 114, 745, 1015, 1131, 1433, 2190, 2547, 2637
NmuCI GTSAC 3 cut(s) 353, 2036, 2470
NsiI ATGCAT 2 cut(s) 297, 1807
NspI RCATGY 4 cut(s) 295, 1375, 2206, 3092
NspV TTCGAA 1 cut(s) 209
OliI CACNNNNGTG 1 cut(s) 2255
PacI TTAATTAA 1 cut(s) 3114
PagI TCATGA 1 cut(s) 1975
PciI ACATGT 1 cut(s) 1371
PdmI GAANNNNTTC 3 cut(s) 1464, 2013, 2290
PflFI GACNNNGTC 1 cut(s) 2969
PflMI CCANNNNNTGG 1 cut(s) 2003
PfoI TCCNGGA 1 cut(s) 840
PinAI ACCGGT 1 cut(s) 527
PkrI GCNGC 8 cut(s) 419, 435, 474, 1334, 1436, 1485, 1738, 2693
PleI GAGTC 4 cut(s) 129, 337, 567, 1885
PpsI GAGTC 4 cut(s) 129, 337, 567, 1885
Ppu21I YACGTR 2 cut(s) 2464, 2912
PpuMI RGGWCCY 1 cut(s) 1014
PscI ACATGT 1 cut(s) 1371
PshBI ATTAAT 2 cut(s) 2319, 3114
PsiI TTATAA 2 cut(s) 300, 2160
Psp124BI GAGCTC 1 cut(s) 270
Psp1406I AACGTT 1 cut(s) 3154
Psp5II RGGWCCY 1 cut(s) 1014
PspFI CCCAGC 1 cut(s) 475
PspN4I GGNNCC 8 cut(s) 114, 745, 1015, 1131, 1433, 2190, 2547, 2637
PspPI GGNCC 5 cut(s) 103, 112, 1014, 1432, 2957
PspPPI RGGWCCY 1 cut(s) 1014
PstI CTGCAG 1 cut(s) 513
PstNI CAGNNNCTG 2 cut(s) 124, 2855
PsuI RGATCY 2 cut(s) 1129, 2545
PsyI GACNNNGTC 1 cut(s) 2969
PvuII CAGCTG 2 cut(s) 475, 1631
RsaI GTAC 4 cut(s) 128, 681, 942, 1763
RsaNI GTAC 4 cut(s) 127, 680, 941, 1762
RseI CAYNNNNRTG 5 cut(s) 63, 789, 2255, 2466, 3093
SacI GAGCTC 1 cut(s) 270
SacII CCGCGG 1 cut(s) 1438
SatI GCNGC 8 cut(s) 418, 434, 473, 1333, 1435, 1484, 1737, 2692
Sau3AI GATC 4 cut(s) 577, 815, 1129, 2545
Sau96I GGNCC 5 cut(s) 103, 112, 1014, 1432, 2957
SchI GAGTC 4 cut(s) 130, 337, 567, 1886
ScrFI CCNGG 1 cut(s) 842
SduI GDGCHC 3 cut(s) 88, 270, 887
SfcI CTRYAG 2 cut(s) 509, 963
Sfr303I CCGCGG 1 cut(s) 1438
SfuI TTCGAA 1 cut(s) 209
SgrBI CCGCGG 1 cut(s) 1438
SinI GGWCC 3 cut(s) 112, 1014, 2957
SmiMI CAYNNNNRTG 5 cut(s) 63, 789, 2255, 2466, 3093
SmlI CTYRAG 4 cut(s) 269, 1109, 1119, 1724
SmoI CTYRAG 4 cut(s) 269, 1109, 1119, 1724
SnaBI TACGTA 1 cut(s) 2912
SsiI CCGC 5 cut(s) 197, 1435, 1437, 2172, 2691
SspI AATATT 2 cut(s) 214, 1284
SspMI CTAG 3 cut(s) 236, 1536, 1751
SstI GAGCTC 1 cut(s) 270
StyD4I CCNGG 1 cut(s) 840
StyI CCWWGG 1 cut(s) 148
TaaI ACNGT 9 cut(s) 13, 145, 949, 1300, 1779, 2330, 2497, 2841, 2857
TaiI ACGT 6 cut(s) 170, 1176, 2466, 2914, 2973, 3157
TaqI TCGA 6 cut(s) 209, 576, 818, 887, 2355, 2646
TatI WGTACW 1 cut(s) 1761
TauI GCSGC 2 cut(s) 1437, 2694
TscAI CASTG 6 cut(s) 358, 502, 1963, 2041, 2143, 2967
TseFI GTSAC 3 cut(s) 353, 2036, 2470
TseI GCWGC 6 cut(s) 417, 433, 472, 1332, 1483, 1736
Tsp45I GTSAC 3 cut(s) 353, 2036, 2470
TspGWI ACGGA 4 cut(s) 723, 1858, 2685, 3075
TspRI CASTG 6 cut(s) 358, 502, 1963, 2041, 2143, 2967
Tth111I GACNNNGTC 1 cut(s) 2969
Van91I CCANNNNNTGG 1 cut(s) 2003
VpaK11BI GGWCC 3 cut(s) 112, 1014, 2957
VspI ATTAAT 2 cut(s) 2319, 3114
XagI CCTNNNNNAGG 1 cut(s) 1384
XapI RAATTY 8 cut(s) 969, 1150, 1396, 1675, 2287, 2347, 2365, 2661
XceI RCATGY 4 cut(s) 295, 1375, 2206, 3092
XcmI CCANNNNNNNNNTGG 2 cut(s) 1738, 1913
XmiI GTMKAC 2 cut(s) 799, 2579
XmnI GAANNNNTTC 3 cut(s) 1464, 2013, 2290
XspI CTAG 3 cut(s) 236, 1536, 1751
Zsp2I ATGCAT 2 cut(s) 297, 1807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.