MD07G1257200.v1.1

receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012362
Physical Location & Seq
Forward (+)
32357206 .. 32360151
2946 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1257200.v1.1.491

Sequence Viewer

Length: 2043 bp
ATGGGTTTTGCAGTTCTTCTCTTCTCCTTCCTGATTTTCTTCACAAACCCGTTATGGGTTTGTGGAAACGCAGAGCTAAAAGCACTTTTGGACATGAAAGCGCTTTTGGACCCGCAAAGCTTGTACCTTTCTTCTTGGACCGTCAGTGGCGACCCCTGCGACGGGTCCTTTGAGGGAATAGGGTGCAATGAGGCGGGTCAAGTTGCTAACATTTCGCTGCAGGGAAAGAGGCTCTCTGGGAAGCTCTCTCCCGCCATTGCAGAGCTCAAGCATTTGACGGGGATTTACATGCATTACAACTCTCTTCAAGGAGAGATTCCAAGGGAGATTGCTAACTTGACGGAGCTGAGTGACTTGTATTTGAATGTGAATAATCTCTCCGGAGTGATTCCTCCCGAGATCGGAAGCATGAGCAGCTTGCAAGTTTTGCAACTTTGCTATAATCAGCTCACCGGAAGCATTCCCACGCAGCTGGGGTCTTTGAAAAACCTGAGCGTTCTTGCTCTGCAATCTAATCAATTAACCGGCGCAATTCCGGCTAGTTTGGGGGACCTAGAAATGTTGATGAGACTGGATTTGAGTGTCAATCATCTTTTCGGTTCTATTCCCACAAAACTTGCTGACGCGCCGGTGCTTGAAGTTCTAGACGTTCGGAATAATACCCTATCCGGCAATGTACCTCTTGCTTTGAAGAGACTTAATGAAGGATTCTCTTACGAAAACAATCTGGGATTATGTGGAGCCGGATTCATGTCCTTAACCGCCTGCACTACCTCCGGTCATCTCAACACGAGTAGACCTGAGCCCTTTGGAGCCGGTGTGCCAACAAGAGACATCCCGGAGACTGCACATGTGCCTTTGCCCTGCAACCAAACTCGTTGCTCGAATTTGTCAAAATCCCATCAGGCCTCTGTTGCTGTAGTTGTTGGTCTCATTGTAGTTACTATTGCATTGTCAGGTGTGGGGTTTCTATTCTTTGCCCAACACCGGCGCAGGAAACAGAAGCTTGGTAACTCATTCGATACAGCTGATGGCCGTCTTAGTACTGACGAGGCCAAGGCCAATGGAGTTTACAGGAAGAATGGATCTCCTCTCATCAGCCTTGAGTACGCCAACGGCTGGGATCCTTTAGCTGATGGGAGGAATTTAAGCGTGTTTGCACAAGAAGTGTTCCAAAGCTTTAGATTCAATTTGGAAGAGGTGGAGACGGCTACCCAGTATTTCTCAGAGGTGAATTTATTGGGTAGGAGTAACTTCTCTGCAACATATAAAGGAATACTACGAGATGGGTCTGTTGTTTCCATTAAGAGCATTGGCAAATCATGCTGCAAGACGGAGGAATCTGAGTTCTTGAAGGGATTGAACATGTTAACCTCGTTGACGCATGAAAATTTGGTGAGGTTGAGAGGGTTCTGTTGCTCAAAGGGTCGCGGAGAATGCTTCCTCATTTATGATTTCGTTCCTAATGGAAATTTGCAGCAGTATCTTGATGTCAAGGACGGTGACAGCCATGCTCTTGAATGGACTACTAGGGTTTCGATTGTGAAGGGCATTGCGAAAGGTCTATCTTATTTACATGGATACAAGCCAAACAAACCTGCCCTTGTTCACCAAAACATCTCAGCTGAAAAGGTGCAGATCGACGAGCAATACAACCCCTTGCTCTCAGATCCTGGCCTGCACAAGCTTCTCACCAACGATGTTGTCTTCTCTGCGCTCAAGGCCAGTGCTGCCATGGGATACCTAGCTCCCGAGTACACCACCACAGGACGGTTCACAGAGAAAAGTGACGTCTATGCATTTGGGGTGCTTGTTCTCCAAATTCTATCAGGGAAGCGGAAAGTCACCAGCTCAATGCGCCTTGGTGCTGAGTCCGGCAGATTCCAAGATTTCATCGACCAAAACCTCAATGGCAGGTTCTTCGAGTACGAAGCGTCCAAGCTTGCAAAGACTGCTCTGCTCTGCACCCACGAGTCTCCAATTGAGAGACCCTCCATGGAAGAAGTTGTTCAGGAGTTGAGTAATTGTAGCAGTTGTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase_Tyr

Protein Analysis

681

Amino Acids

74.1

Weight (kDa)

6.16

Isoelectric Point (pI)

34.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 23 - 63 1.7e-07 Leucine rich repeat N-terminal domain
LRR_14 PF23598 80 - 199 3.4e-09 Leucine-rich repeat region
LRR_8 PF13855 139 - 198 4.2e-08 Leucine rich repeat
Pkinase PF00069 409 - 671 1.6e-23 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 412 - 673 8.5e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51560
fragaria_vesca FvH4_7g28070
malus_domestica MD01G1184900.v1.1 MD07G1257200.v1.1
prunus_persica Prupe.2G281400_v2.0.a1
pyrus_communis pycom01g19660 pycom07g23010 pycom07g23020
rosa_chinensis RchiOBHm_Chr1g0375021
rosa_laevigata RLG00000026697
rosa_multiflora Rmu_sc0000554.1_g000005 Rmu_sc0017099.1_g000006
rosa_roxburghii Rroxscaffold_4G00283080
rosa_rugosa Rorug01G0387500
rosa_samantha Rh1AG398100 Rh1BG362000 Rh1CG375700 Rh1DG393200
rosa_wichuraiana Rw1G035330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1794
Acc36I ACCTGC 2 cut(s) 1606, 1905
AccB7I CCANNNNNTGG 1 cut(s) 1119
AccI GTMKAC 1 cut(s) 796
AccII CGCG 2 cut(s) 626, 1431
AccIII TCCGGA 1 cut(s) 380
AciI CCGC 6 cut(s) 113, 194, 252, 762, 1431, 1837
AclWI GGATC 4 cut(s) 1093, 1118, 1131, 1664
AcoI YGGCCR 1 cut(s) 1033
AcsI RAATTY 6 cut(s) 886, 1144, 1234, 1390, 1471, 1821
AcyI GRCGYC 1 cut(s) 1791
AfaI GTAC 6 cut(s) 125, 678, 1045, 1109, 1757, 1928
AfeI AGCGCT 1 cut(s) 102
AfiI CCNNNNNNNGG 7 cut(s) 55, 161, 162, 401, 987, 1119, 1770
AflIII ACRYGT 2 cut(s) 850, 1365
AgsI TTSAA 9 cut(s) 308, 364, 484, 638, 691, 1189, 1354, 1363, 1520
AjnI CCWGG 1 cut(s) 1672
AjuI GAANNNNNNNTTGG 2 cut(s) 89, 121
AloI GAACNNNNNNTCC 2 cut(s) 1331, 1363
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 8 cut(s) 562, 688, 825, 836, 935, 1199, 1980, 1981
AlwI GGATC 4 cut(s) 1093, 1118, 1131, 1664
AlwNI CAGNNNCTG 1 cut(s) 1673
Ama87I CYCGRG 2 cut(s) 395, 1751
Aor13HI TCCGGA 1 cut(s) 380
Aor51HI AGCGCT 1 cut(s) 102
AoxI GGCC 6 cut(s) 906, 1033, 1053, 1059, 1675, 1722
ApeKI GCWGC 6 cut(s) 217, 414, 469, 1326, 1477, 1730
ApoI RAATTY 6 cut(s) 886, 1144, 1234, 1390, 1471, 1821
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
Asp700I GAANNNNTTC 1 cut(s) 2007
AspLEI GCGC 6 cut(s) 103, 530, 628, 993, 1717, 1860
AspS9I GGNCC 4 cut(s) 109, 138, 165, 550
AsuC2I CCSGG 1 cut(s) 839
AsuHPI GGTGA 7 cut(s) 442, 1243, 1408, 1514, 1601, 1684, 1837
AvaI CYCGRG 2 cut(s) 395, 1751
AvaII GGWCC 4 cut(s) 109, 138, 165, 550
BamHI GGATCC 1 cut(s) 1123
BanII GRGCYC 2 cut(s) 267, 807
BauI CACGAG 2 cut(s) 790, 1970
BbsI GAAGAC 1 cut(s) 1699
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 6 cut(s) 204, 426, 481, 1313, 1489, 1717
BccI CCATC 4 cut(s) 909, 1025, 1130, 1280
BceAI ACGGC 3 cut(s) 1020, 1132, 1224
BcgI CGANNNNNNTGC 2 cut(s) 1903, 1937
BciT130I CCWGG 1 cut(s) 1674
BciVI GTATCC 2 cut(s) 1574, 1733
BcnI CCSGG 1 cut(s) 839
BcoDI GTCTC 8 cut(s) 562, 688, 825, 836, 935, 1199, 1980, 1981
BfaI CTAG 5 cut(s) 540, 554, 644, 1530, 1745
BfmI CTRYAG 2 cut(s) 218, 918
BfoI RGCGCY 1 cut(s) 104
BfuAI ACCTGC 2 cut(s) 1606, 1905
BfuI GTATCC 2 cut(s) 1574, 1733
BisI GCNGC 6 cut(s) 218, 415, 470, 1327, 1478, 1731
BlsI GCNGC 6 cut(s) 219, 416, 471, 1328, 1479, 1732
BmcAI AGTACT 1 cut(s) 1045
Bme1390I CCNGG 2 cut(s) 839, 1674
Bme18I GGWCC 4 cut(s) 109, 138, 165, 550
BmeT110I CYCGRG 2 cut(s) 395, 1751
BmgT120I GGNCC 4 cut(s) 109, 138, 165, 550
BmiI GGNNCC 6 cut(s) 111, 166, 551, 742, 814, 1125
BmrFI CCNGG 2 cut(s) 839, 1674
BmrI ACTGGG 1 cut(s) 1210
BmuI ACTGGG 1 cut(s) 1210
BpiI GAAGAC 1 cut(s) 1699
BplI GAGNNNNNCTC 2 cut(s) 1976, 2008
Bpu10I CCTNAGC 2 cut(s) 491, 801
BpuEI CTTGAG 3 cut(s) 251, 1124, 1703
BpuMI CCSGG 1 cut(s) 839
BsaHI GRCGYC 1 cut(s) 1791
BsaI GGTCTC 2 cut(s) 935, 1981
BsaJI CCNNGG 5 cut(s) 320, 1056, 1734, 1861, 1995
BsaWI WCCGGW 3 cut(s) 380, 452, 776
BsaXI ACNNNNNCTCC 2 cut(s) 804, 834
Bsc4I CCNNNNNNNGG 7 cut(s) 55, 161, 162, 401, 987, 1119, 1770
Bse118I RCCGGY 4 cut(s) 524, 628, 815, 987
Bse1I ACTGG 3 cut(s) 576, 1216, 1725
Bse3DI GCAATG 4 cut(s) 193, 255, 679, 1551
BseAI TCCGGA 1 cut(s) 380
BseBI CCWGG 1 cut(s) 1674
BseDI CCNNGG 5 cut(s) 320, 1056, 1734, 1861, 1995
BseGI GGATG 1 cut(s) 834
BseLI CCNNNNNNNGG 7 cut(s) 55, 161, 162, 401, 987, 1119, 1770
BseMI GCAATG 4 cut(s) 193, 255, 679, 1551
BseMII CTCAG 8 cut(s) 338, 482, 792, 1239, 1335, 1635, 1680, 1860
BseNI ACTGG 3 cut(s) 576, 1216, 1725
BseRI GAGGAG 1 cut(s) 1080
BseXI GCAGC 6 cut(s) 204, 426, 481, 1313, 1489, 1717
BseYI CCCAGC 2 cut(s) 472, 1119
BsgI GTGCAG 5 cut(s) 751, 831, 1655, 1664, 1948
Bsh1236I CGCG 2 cut(s) 626, 1431
BshFI GGCC 6 cut(s) 908, 1035, 1055, 1061, 1677, 1724
BsiHKAI GWGCWC 1 cut(s) 267
BsiHKCI CYCGRG 2 cut(s) 395, 1751
BslFI GGGAC 1 cut(s) 563
BslI CCNNNNNNNGG 7 cut(s) 55, 161, 162, 401, 987, 1119, 1770
BsmAI GTCTC 8 cut(s) 562, 688, 825, 836, 935, 1199, 1980, 1981
BsmBI CGTCTC 1 cut(s) 1199
BsmFI GGGAC 1 cut(s) 563
BsmI GAATGC 2 cut(s) 459, 1442
BsnI GGCC 6 cut(s) 908, 1035, 1055, 1061, 1677, 1724
Bso31I GGTCTC 2 cut(s) 935, 1981
BsoBI CYCGRG 2 cut(s) 395, 1751
Bsp1286I GDGCHC 2 cut(s) 267, 807
Bsp13I TCCGGA 1 cut(s) 380
Bsp143I GATC 5 cut(s) 399, 1085, 1123, 1638, 1669
Bsp19I CCATGG 2 cut(s) 1734, 1995
BspACI CCGC 6 cut(s) 113, 194, 252, 762, 1431, 1837
BspANI GGCC 6 cut(s) 908, 1035, 1055, 1061, 1677, 1724
BspCNI CTCAG 8 cut(s) 339, 483, 793, 1238, 1336, 1634, 1679, 1861
BspEI TCCGGA 1 cut(s) 380
BspFNI CGCG 2 cut(s) 626, 1431
BspLI GGNNCC 6 cut(s) 111, 166, 551, 742, 814, 1125
BspMAI CTGCAG 1 cut(s) 222
BspMI ACCTGC 2 cut(s) 1606, 1905
BspPI GGATC 4 cut(s) 1093, 1118, 1131, 1664
BspTNI GGTCTC 2 cut(s) 935, 1981
BsrDI GCAATG 4 cut(s) 193, 255, 679, 1551
BsrFI RCCGGY 4 cut(s) 524, 628, 815, 987
BsrI ACTGG 3 cut(s) 576, 1216, 1725
BssAI RCCGGY 4 cut(s) 524, 628, 815, 987
BssECI CCNNGG 5 cut(s) 320, 1056, 1734, 1861, 1995
BssMI GATC 5 cut(s) 399, 1085, 1123, 1638, 1669
BssNI GRCGYC 1 cut(s) 1791
BssSI CACGAG 2 cut(s) 790, 1970
BssT1I CCWWGG 5 cut(s) 320, 1056, 1734, 1861, 1995
Bst2BI CACGAG 2 cut(s) 790, 1970
Bst2UI CCWGG 1 cut(s) 1674
Bst4CI ACNGT 3 cut(s) 142, 1502, 1773
Bst6I CTCTTC 4 cut(s) 26, 309, 686, 1191
BstACI GRCGYC 1 cut(s) 1791
BstAPI GCANNNNNTGC 3 cut(s) 427, 1323, 1952
BstC8I GCNNGC 4 cut(s) 419, 766, 1679, 1944
BstDEI CTNAG 9 cut(s) 347, 491, 801, 1040, 1225, 1344, 1621, 1666, 1869
BstDSI CCRYGG 2 cut(s) 1734, 1995
BstF5I GGATG 1 cut(s) 834
BstFNI CGCG 2 cut(s) 626, 1431
BstH2I RGCGCY 1 cut(s) 104
BstHHI GCGC 6 cut(s) 103, 530, 628, 993, 1717, 1860
BstKTI GATC 5 cut(s) 402, 1088, 1126, 1641, 1672
BstMAI GTCTC 8 cut(s) 562, 688, 825, 836, 935, 1199, 1980, 1981
BstMBI GATC 5 cut(s) 399, 1085, 1123, 1638, 1669
BstNI CCWGG 1 cut(s) 1674
BstNSI RCATGY 3 cut(s) 292, 854, 1369
BstSCI CCNGG 2 cut(s) 837, 1672
BstSFI CTRYAG 2 cut(s) 218, 918
BstUI CGCG 2 cut(s) 626, 1431
BstV1I GCAGC 6 cut(s) 204, 426, 481, 1313, 1489, 1717
BstV2I GAAGAC 1 cut(s) 1699
BstX2I RGATCY 3 cut(s) 1085, 1123, 1669
BstXI CCANNNNNNTGG 1 cut(s) 472
BstYI RGATCY 3 cut(s) 1085, 1123, 1669
BsuI GTATCC 2 cut(s) 1574, 1733
BsuRI GGCC 6 cut(s) 908, 1035, 1055, 1061, 1677, 1724
BtgI CCRYGG 2 cut(s) 1734, 1995
BtsCI GGATG 1 cut(s) 834
BtsIMutI CAGTG 2 cut(s) 151, 1732
BveI ACCTGC 2 cut(s) 1606, 1905
Cac8I GCNNGC 4 cut(s) 419, 766, 1679, 1944
CaiI CAGNNNCTG 1 cut(s) 1673
CfoI GCGC 6 cut(s) 103, 530, 628, 993, 1717, 1860
Cfr10I RCCGGY 4 cut(s) 524, 628, 815, 987
Cfr13I GGNCC 4 cut(s) 109, 138, 165, 550
CseI GACGC 3 cut(s) 632, 1390, 1923
Csp6I GTAC 6 cut(s) 124, 677, 1044, 1108, 1756, 1927
CspCI CAANNNNNGTGG 2 cut(s) 598, 633
CviQI GTAC 6 cut(s) 124, 677, 1044, 1108, 1756, 1927
DdeI CTNAG 9 cut(s) 347, 491, 801, 1040, 1225, 1344, 1621, 1666, 1869
DpnI GATC 5 cut(s) 401, 1087, 1125, 1640, 1671
DpnII GATC 5 cut(s) 399, 1085, 1123, 1638, 1669
EaeI YGGCCR 1 cut(s) 1033
Eam1104I CTCTTC 4 cut(s) 26, 309, 686, 1191
EarI CTCTTC 4 cut(s) 26, 309, 686, 1191
Ecl136II GAGCTC 1 cut(s) 265
Eco130I CCWWGG 5 cut(s) 320, 1056, 1734, 1861, 1995
Eco147I AGGCCT 1 cut(s) 908
Eco24I GRGCYC 2 cut(s) 267, 807
Eco31I GGTCTC 2 cut(s) 935, 1981
Eco47I GGWCC 4 cut(s) 109, 138, 165, 550
Eco47III AGCGCT 1 cut(s) 102
Eco53kI GAGCTC 1 cut(s) 265
Eco88I CYCGRG 2 cut(s) 395, 1751
EcoICRI GAGCTC 1 cut(s) 265
EcoO109I RGGNCCY 2 cut(s) 165, 550
EcoRII CCWGG 1 cut(s) 1672
EcoT14I CCWWGG 5 cut(s) 320, 1056, 1734, 1861, 1995
EcoT22I ATGCAT 2 cut(s) 294, 1801
EcoT38I GRGCYC 2 cut(s) 267, 807
ErhI CCWWGG 5 cut(s) 320, 1056, 1734, 1861, 1995
Esp3I CGTCTC 1 cut(s) 1199
FaqI GGGAC 1 cut(s) 563
FauI CCCGC 3 cut(s) 120, 187, 259
FblI GTMKAC 1 cut(s) 796
Fnu4HI GCNGC 6 cut(s) 218, 415, 470, 1327, 1478, 1731
FokI GGATG 1 cut(s) 821
FriOI GRGCYC 2 cut(s) 267, 807
Fsp4HI GCNGC 6 cut(s) 218, 415, 470, 1327, 1478, 1731
FspBI CTAG 5 cut(s) 540, 554, 644, 1530, 1745
GlaI GCGC 6 cut(s) 102, 529, 627, 992, 1716, 1859
GluI GCNGC 6 cut(s) 218, 415, 470, 1327, 1478, 1731
GsaI CCCAGC 2 cut(s) 476, 1123
HaeII RGCGCY 1 cut(s) 104
HaeIII GGCC 6 cut(s) 908, 1035, 1055, 1061, 1677, 1724
HgaI GACGC 3 cut(s) 632, 1390, 1923
HhaI GCGC 6 cut(s) 103, 530, 628, 993, 1717, 1860
Hin1I GRCGYC 1 cut(s) 1791
Hin6I GCGC 6 cut(s) 101, 528, 626, 991, 1715, 1858
HinP1I GCGC 6 cut(s) 101, 528, 626, 991, 1715, 1858
HincII GTYRAC 2 cut(s) 1371, 1380
HindII GTYRAC 2 cut(s) 1371, 1380
HindIII AAGCTT 5 cut(s) 118, 1004, 1177, 1685, 1940
HinfI GANTC 9 cut(s) 316, 388, 708, 747, 1185, 1340, 1871, 1881, 1973
HpaI GTTAAC 1 cut(s) 1371
HphI GGTGA 7 cut(s) 442, 1243, 1408, 1514, 1601, 1684, 1837
Hpy166II GTNNAC 7 cut(s) 797, 1072, 1371, 1380, 1609, 1758, 1776
Hpy188I TCNGA 6 cut(s) 404, 654, 1228, 1345, 1669, 2042
Hpy188III TCNNGA 9 cut(s) 31, 381, 395, 644, 1351, 1487, 1517, 1751, 2012
Hpy8I GTNNAC 7 cut(s) 797, 1072, 1371, 1380, 1609, 1758, 1776
Hpy99I CGWCG 2 cut(s) 164, 1646
HpyAV CCTTC 4 cut(s) 37, 698, 1348, 1540
HpyCH4III ACNGT 3 cut(s) 142, 1502, 1773
HpyCH4IV ACGT 2 cut(s) 648, 1791
HpyF3I CTNAG 9 cut(s) 347, 491, 801, 1040, 1225, 1344, 1621, 1666, 1869
HpySE526I ACGT 2 cut(s) 648, 1791
Hsp92I GRCGYC 1 cut(s) 1791
HspAI GCGC 6 cut(s) 101, 528, 626, 991, 1715, 1858
Kpn2I TCCGGA 1 cut(s) 380
KspAI GTTAAC 1 cut(s) 1371
Kzo9I GATC 5 cut(s) 399, 1085, 1123, 1638, 1669
LmnI GCTCC 4 cut(s) 343, 740, 812, 1753
Lsp1109I GCAGC 6 cut(s) 204, 426, 481, 1313, 1489, 1717
MaeI CTAG 5 cut(s) 540, 554, 644, 1530, 1745
MaeII ACGT 2 cut(s) 648, 1791
MaeIII GTNAC 7 cut(s) 350, 940, 1010, 1250, 1502, 1787, 1843
MalI GATC 5 cut(s) 401, 1087, 1125, 1640, 1671
MboI GATC 5 cut(s) 399, 1085, 1123, 1638, 1669
MfeI CAATTG 1 cut(s) 1980
MflI RGATCY 3 cut(s) 1085, 1123, 1669
MhlI GDGCHC 2 cut(s) 267, 807
MlyI GAGTC 2 cut(s) 1880, 1982
Mph1103I ATGCAT 2 cut(s) 294, 1801
MroI TCCGGA 1 cut(s) 380
MroXI GAANNNNTTC 1 cut(s) 2007
MseI TTAA 6 cut(s) 521, 699, 758, 1148, 1305, 1370
MspA1I CMGCKG 3 cut(s) 472, 1028, 1625
MspR9I CCNGG 2 cut(s) 839, 1674
MunI CAATTG 1 cut(s) 1980
Mva1269I GAATGC 2 cut(s) 459, 1442
MvaI CCWGG 1 cut(s) 1674
MvnI CGCG 2 cut(s) 626, 1431
NciI CCSGG 1 cut(s) 839
NcoI CCATGG 2 cut(s) 1734, 1995
NdeII GATC 5 cut(s) 399, 1085, 1123, 1638, 1669
NlaIV GGNNCC 6 cut(s) 111, 166, 551, 742, 814, 1125
NmuCI GTSAC 4 cut(s) 350, 1502, 1787, 1843
NsiI ATGCAT 2 cut(s) 294, 1801
NspI RCATGY 3 cut(s) 292, 854, 1369
PceI AGGCCT 1 cut(s) 908
PciI ACATGT 2 cut(s) 850, 1365
PctI GAATGC 2 cut(s) 459, 1442
PdmI GAANNNNTTC 1 cut(s) 2007
PfeI GAWTC 7 cut(s) 316, 388, 708, 747, 1185, 1340, 1881
PflMI CCANNNNNTGG 1 cut(s) 1119
PfoI TCCNGGA 1 cut(s) 837
PkrI GCNGC 6 cut(s) 219, 416, 471, 1328, 1479, 1732
PleI GAGTC 2 cut(s) 1879, 1981
PpsI GAGTC 2 cut(s) 1879, 1981
PpuMI RGGWCCY 2 cut(s) 165, 550
PscI ACATGT 2 cut(s) 850, 1365
Psp124BI GAGCTC 1 cut(s) 267
Psp5II RGGWCCY 2 cut(s) 165, 550
Psp6I CCWGG 1 cut(s) 1672
PspFI CCCAGC 2 cut(s) 472, 1119
PspGI CCWGG 1 cut(s) 1672
PspN4I GGNNCC 6 cut(s) 111, 166, 551, 742, 814, 1125
PspPI GGNCC 4 cut(s) 109, 138, 165, 550
PspPPI RGGWCCY 2 cut(s) 165, 550
PstI CTGCAG 1 cut(s) 222
PstNI CAGNNNCTG 1 cut(s) 1673
PsuI RGATCY 3 cut(s) 1085, 1123, 1669
PvuII CAGCTG 3 cut(s) 472, 1028, 1625
RsaI GTAC 6 cut(s) 125, 678, 1045, 1109, 1757, 1928
RsaNI GTAC 6 cut(s) 124, 677, 1044, 1108, 1756, 1927
SacI GAGCTC 1 cut(s) 267
SaqAI TTAA 6 cut(s) 521, 699, 758, 1148, 1305, 1370
SatI GCNGC 6 cut(s) 218, 415, 470, 1327, 1478, 1731
Sau3AI GATC 5 cut(s) 399, 1085, 1123, 1638, 1669
Sau96I GGNCC 4 cut(s) 109, 138, 165, 550
ScaI AGTACT 1 cut(s) 1045
SchI GAGTC 2 cut(s) 1880, 1982
ScrFI CCNGG 2 cut(s) 839, 1674
SduI GDGCHC 2 cut(s) 267, 807
SfcI CTRYAG 2 cut(s) 218, 918
SgrAI CRCCGGYG 2 cut(s) 628, 987
SinI GGWCC 4 cut(s) 109, 138, 165, 550
SmlI CTYRAG 3 cut(s) 266, 1103, 1718
SmoI CTYRAG 3 cut(s) 266, 1103, 1718
SseBI AGGCCT 1 cut(s) 908
SsiI CCGC 6 cut(s) 113, 194, 252, 762, 1431, 1837
SspMI CTAG 5 cut(s) 540, 554, 644, 1530, 1745
SstI GAGCTC 1 cut(s) 267
StuI AGGCCT 1 cut(s) 908
StyD4I CCNGG 2 cut(s) 837, 1672
StyI CCWWGG 5 cut(s) 320, 1056, 1734, 1861, 1995
TaaI ACNGT 3 cut(s) 142, 1502, 1773
TaiI ACGT 2 cut(s) 651, 1794
TaqI TCGA 6 cut(s) 884, 1020, 1538, 1641, 1896, 1923
TatI WGTACW 2 cut(s) 1043, 1755
TfiI GAWTC 7 cut(s) 316, 388, 708, 747, 1185, 1340, 1881
Tru1I TTAA 6 cut(s) 521, 699, 758, 1148, 1305, 1370
Tru9I TTAA 6 cut(s) 521, 699, 758, 1148, 1305, 1370
TscAI CASTG 2 cut(s) 151, 1732
TseFI GTSAC 4 cut(s) 350, 1502, 1787, 1843
TseI GCWGC 6 cut(s) 217, 414, 469, 1326, 1477, 1730
Tsp45I GTSAC 4 cut(s) 350, 1502, 1787, 1843
TspDTI ATGAA 5 cut(s) 110, 717, 739, 1401, 1882
TspGWI ACGGA 2 cut(s) 356, 1349
TspRI CASTG 2 cut(s) 151, 1732
Van91I CCANNNNNTGG 1 cut(s) 1119
VpaK11BI GGWCC 4 cut(s) 109, 138, 165, 550
XapI RAATTY 6 cut(s) 886, 1144, 1234, 1390, 1471, 1821
XbaI TCTAGA 1 cut(s) 643
XceI RCATGY 3 cut(s) 292, 854, 1369
XcmI CCANNNNNNNNNTGG 2 cut(s) 1732, 1907
XmiI GTMKAC 1 cut(s) 796
XmnI GAANNNNTTC 1 cut(s) 2007
XspI CTAG 5 cut(s) 540, 554, 644, 1530, 1745
ZraI GACGTC 1 cut(s) 1792
ZrmI AGTACT 1 cut(s) 1045
Zsp2I ATGCAT 2 cut(s) 294, 1801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.