pycom07g23020

receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0012362
Physical Location & Seq
Forward (+)
24113954 .. 24114737
784 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g23020.1

Sequence Viewer

Length: 678 bp
ATGTTAACCTCGTTGAGGCATGAAAATTTGGTGAGCTTGAGAGGGTTCTGTTGCTCAAGGGGCCGCGGAGAATGCTTCCTCATTTATGATTTCGTTCCTAATGGAAATTTGCTGCAGTATCTTGATGTCAAGGACGGCGACAGCCATGTTCTTGAATGGACTACTAGGGTTTCTATTGTGAAGGGCATTGCTAAAGGTCTGTCTTATTTACATGGATACAAGCCAAACAAACCTGCCCTTGTTCACCAAAACATCTCAGCTGAAAAGGTGCAGATCGACCAGCGATACAGCCCCCTGCTCTCAGATCCTGGCCTGCACAAACTTCTCACCAATGATGTTGTTTTCTCTGCGCTCAAGGCCAGTGCTGCCATGGGATACCTAGCTCCCGAGTACACCACCACAGGACGGTTCACAGAGAAAAGTGACGTCTATGCATTTGGGGTGCTTGTTCTCCAAATTCTATCAGGGAAGCGGAAAGTCACCAGCTCAATGCGCCTTGGTGCTGAGTCGGGCAGATTCCAAGATTTCATTGACCAAAACCTCAATGGCAGGTTCTTTGAGTACGAAGCGTCCAAGCTTGCAAAGACTGCTCTGCTCTGCACCCACGAGTCTCCAATTGAGAGACCCTCCATGGAAGAAGTTGTTCAGGAGTTGAGTAATTGTAGCAGTTGTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase_Tyr

Protein Analysis

226

Amino Acids

25.06

Weight (kDa)

7.63

Isoelectric Point (pI)

33.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 1 - 218 5e-30 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 1 - 216 1.5e-17 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51560
fragaria_vesca FvH4_7g28070
malus_domestica MD01G1184900.v1.1 MD07G1257200.v1.1
prunus_persica Prupe.2G281400_v2.0.a1
pyrus_communis pycom01g19660 pycom07g23010 pycom07g23020
rosa_chinensis RchiOBHm_Chr1g0375021
rosa_laevigata RLG00000026697
rosa_multiflora Rmu_sc0000554.1_g000005 Rmu_sc0017099.1_g000006
rosa_roxburghii Rroxscaffold_4G00283080
rosa_rugosa Rorug01G0387500
rosa_samantha Rh1AG398100 Rh1BG362000 Rh1CG375700 Rh1DG393200
rosa_wichuraiana Rw1G035330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 429
Acc36I ACCTGC 2 cut(s) 241, 540
AccII CGCG 1 cut(s) 66
AciI CCGC 3 cut(s) 64, 66, 472
AclWI GGATC 1 cut(s) 299
AcsI RAATTY 3 cut(s) 25, 106, 456
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 2 cut(s) 392, 563
AfiI CCNNNNNNNGG 2 cut(s) 15, 405
AgsI TTSAA 1 cut(s) 155
AjnI CCWGG 1 cut(s) 307
AluBI AGCT 5 cut(s) 36, 260, 383, 486, 577
AluI AGCT 5 cut(s) 36, 260, 383, 486, 577
Alw26I GTCTC 2 cut(s) 615, 616
AlwI GGATC 1 cut(s) 299
AlwNI CAGNNNCTG 1 cut(s) 308
Ama87I CYCGRG 1 cut(s) 386
AoxI GGCC 3 cut(s) 61, 310, 357
ApeKI GCWGC 2 cut(s) 112, 365
ApoI RAATTY 3 cut(s) 25, 106, 456
Asp700I GAANNNNTTC 1 cut(s) 642
AspLEI GCGC 2 cut(s) 352, 495
AspS9I GGNCC 1 cut(s) 61
AsuHPI GGTGA 4 cut(s) 43, 236, 319, 472
AvaI CYCGRG 1 cut(s) 386
BauI CACGAG 1 cut(s) 605
BbvI GCAGC 2 cut(s) 99, 352
BceAI ACGGC 1 cut(s) 151
BciT130I CCWGG 1 cut(s) 309
BciVI GTATCC 2 cut(s) 209, 368
BcoDI GTCTC 2 cut(s) 615, 616
BfaI CTAG 2 cut(s) 165, 380
BfmI CTRYAG 1 cut(s) 113
BfuAI ACCTGC 2 cut(s) 241, 540
BfuI GTATCC 2 cut(s) 209, 368
BisI GCNGC 3 cut(s) 64, 113, 366
BlsI GCNGC 3 cut(s) 65, 114, 367
Bme1390I CCNGG 1 cut(s) 309
BmeT110I CYCGRG 1 cut(s) 386
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 1 cut(s) 62
BmrFI CCNGG 1 cut(s) 309
BplI GAGNNNNNCTC 2 cut(s) 611, 643
BpuEI CTTGAG 3 cut(s) 40, 58, 338
BsaHI GRCGYC 1 cut(s) 426
BsaI GGTCTC 1 cut(s) 616
BsaJI CCNNGG 4 cut(s) 64, 369, 496, 630
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 405
Bse1I ACTGG 1 cut(s) 360
Bse3DI GCAATG 1 cut(s) 186
BseBI CCWGG 1 cut(s) 309
BseDI CCNNGG 4 cut(s) 64, 369, 496, 630
BseLI CCNNNNNNNGG 2 cut(s) 15, 405
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 3 cut(s) 270, 315, 495
BseNI ACTGG 1 cut(s) 360
BseXI GCAGC 2 cut(s) 99, 352
BsgI GTGCAG 3 cut(s) 290, 299, 583
Bsh1236I CGCG 1 cut(s) 66
BshFI GGCC 3 cut(s) 63, 312, 359
BsiHKCI CYCGRG 1 cut(s) 386
BslI CCNNNNNNNGG 2 cut(s) 15, 405
BsmAI GTCTC 2 cut(s) 615, 616
BsmI GAATGC 1 cut(s) 77
BsnI GGCC 3 cut(s) 63, 312, 359
Bso31I GGTCTC 1 cut(s) 616
BsoBI CYCGRG 1 cut(s) 386
Bsp143I GATC 2 cut(s) 273, 304
Bsp19I CCATGG 2 cut(s) 369, 630
BspACI CCGC 3 cut(s) 64, 66, 472
BspANI GGCC 3 cut(s) 63, 312, 359
BspCNI CTCAG 3 cut(s) 269, 314, 496
BspFNI CGCG 1 cut(s) 66
BspLI GGNNCC 1 cut(s) 62
BspMAI CTGCAG 1 cut(s) 117
BspMI ACCTGC 2 cut(s) 241, 540
BspPI GGATC 1 cut(s) 299
BspTNI GGTCTC 1 cut(s) 616
BsrDI GCAATG 1 cut(s) 186
BsrI ACTGG 1 cut(s) 360
BssECI CCNNGG 4 cut(s) 64, 369, 496, 630
BssMI GATC 2 cut(s) 273, 304
BssNI GRCGYC 1 cut(s) 426
BssSI CACGAG 1 cut(s) 605
BssT1I CCWWGG 3 cut(s) 369, 496, 630
Bst2BI CACGAG 1 cut(s) 605
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 1 cut(s) 408
BstACI GRCGYC 1 cut(s) 426
BstAPI GCANNNNNTGC 1 cut(s) 587
BstC8I GCNNGC 2 cut(s) 314, 579
BstDEI CTNAG 3 cut(s) 256, 301, 504
BstDSI CCRYGG 3 cut(s) 64, 369, 630
BstENI CCTNNNNNAGG 1 cut(s) 13
BstFNI CGCG 1 cut(s) 66
BstHHI GCGC 2 cut(s) 352, 495
BstKTI GATC 2 cut(s) 276, 307
BstMAI GTCTC 2 cut(s) 615, 616
BstMBI GATC 2 cut(s) 273, 304
BstMWI GCNNNNNNNGC 6 cut(s) 60, 72, 356, 365, 492, 587
BstNI CCWGG 1 cut(s) 309
BstSCI CCNGG 1 cut(s) 307
BstSFI CTRYAG 1 cut(s) 113
BstUI CGCG 1 cut(s) 66
BstV1I GCAGC 2 cut(s) 99, 352
BstX2I RGATCY 1 cut(s) 304
BstYI RGATCY 1 cut(s) 304
BsuI GTATCC 2 cut(s) 209, 368
BsuRI GGCC 3 cut(s) 63, 312, 359
BtgI CCRYGG 3 cut(s) 64, 369, 630
BtsIMutI CAGTG 1 cut(s) 367
BveI ACCTGC 2 cut(s) 241, 540
Cac8I GCNNGC 2 cut(s) 314, 579
CaiI CAGNNNCTG 1 cut(s) 308
CfoI GCGC 2 cut(s) 352, 495
Cfr13I GGNCC 1 cut(s) 61
Cfr42I CCGCGG 1 cut(s) 67
CseI GACGC 1 cut(s) 558
Csp6I GTAC 2 cut(s) 391, 562
CviAII CATG 5 cut(s) 20, 146, 212, 370, 631
CviQI GTAC 2 cut(s) 391, 562
DdeI CTNAG 3 cut(s) 256, 301, 504
DpnI GATC 2 cut(s) 275, 306
DpnII GATC 2 cut(s) 273, 304
Eco130I CCWWGG 3 cut(s) 369, 496, 630
Eco31I GGTCTC 1 cut(s) 616
Eco88I CYCGRG 1 cut(s) 386
EcoNI CCTNNNNNAGG 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 307
EcoT14I CCWWGG 3 cut(s) 369, 496, 630
EcoT22I ATGCAT 1 cut(s) 436
ErhI CCWWGG 3 cut(s) 369, 496, 630
FaeI CATG 5 cut(s) 23, 149, 215, 373, 634
FaiI YATR 7 cut(s) 21, 87, 147, 213, 371, 432, 632
FatI CATG 5 cut(s) 19, 145, 211, 369, 630
Fnu4HI GCNGC 3 cut(s) 64, 113, 366
Fsp4HI GCNGC 3 cut(s) 64, 113, 366
FspBI CTAG 2 cut(s) 165, 380
GlaI GCGC 2 cut(s) 351, 494
GluI GCNGC 3 cut(s) 64, 113, 366
HaeIII GGCC 3 cut(s) 63, 312, 359
HgaI GACGC 1 cut(s) 558
HhaI GCGC 2 cut(s) 352, 495
Hin1I GRCGYC 1 cut(s) 426
Hin1II CATG 5 cut(s) 23, 149, 215, 373, 634
Hin6I GCGC 2 cut(s) 350, 493
HinP1I GCGC 2 cut(s) 350, 493
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 575
HinfI GANTC 3 cut(s) 506, 516, 608
HpaI GTTAAC 1 cut(s) 6
HphI GGTGA 4 cut(s) 43, 236, 319, 472
Hpy166II GTNNAC 4 cut(s) 6, 244, 393, 411
Hpy188I TCNGA 2 cut(s) 304, 677
Hpy188III TCNNGA 4 cut(s) 122, 152, 386, 647
Hpy8I GTNNAC 4 cut(s) 6, 244, 393, 411
HpyAV CCTTC 1 cut(s) 175
HpyCH4III ACNGT 1 cut(s) 408
HpyCH4IV ACGT 1 cut(s) 426
HpyCH4V TGCA 6 cut(s) 115, 271, 316, 434, 581, 600
HpyF10VI GCNNNNNNNGC 6 cut(s) 60, 72, 356, 365, 492, 587
HpyF3I CTNAG 3 cut(s) 256, 301, 504
HpySE526I ACGT 1 cut(s) 426
Hsp92I GRCGYC 1 cut(s) 426
Hsp92II CATG 5 cut(s) 23, 149, 215, 373, 634
HspAI GCGC 2 cut(s) 350, 493
KspAI GTTAAC 1 cut(s) 6
KspI CCGCGG 1 cut(s) 67
Kzo9I GATC 2 cut(s) 273, 304
LmnI GCTCC 1 cut(s) 388
Lsp1109I GCAGC 2 cut(s) 99, 352
MaeI CTAG 2 cut(s) 165, 380
MaeII ACGT 1 cut(s) 426
MaeIII GTNAC 2 cut(s) 422, 478
MalI GATC 2 cut(s) 275, 306
MboI GATC 2 cut(s) 273, 304
MboII GAAGA 1 cut(s) 647
MfeI CAATTG 1 cut(s) 615
MflI RGATCY 1 cut(s) 304
MluCI AATT 5 cut(s) 25, 106, 456, 615, 658
MlyI GAGTC 2 cut(s) 515, 617
MnlI CCTC 6 cut(s) 9, 19, 35, 89, 551, 637
Mph1103I ATGCAT 1 cut(s) 436
MroXI GAANNNNTTC 1 cut(s) 642
MseI TTAA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 66, 260
MspR9I CCNGG 1 cut(s) 309
MunI CAATTG 1 cut(s) 615
Mva1269I GAATGC 1 cut(s) 77
MvaI CCWGG 1 cut(s) 309
MvnI CGCG 1 cut(s) 66
MwoI GCNNNNNNNGC 6 cut(s) 60, 72, 356, 365, 492, 587
NcoI CCATGG 2 cut(s) 369, 630
NdeII GATC 2 cut(s) 273, 304
NlaIII CATG 5 cut(s) 23, 149, 215, 373, 634
NlaIV GGNNCC 1 cut(s) 62
NmuCI GTSAC 2 cut(s) 422, 478
NsiI ATGCAT 1 cut(s) 436
PctI GAATGC 1 cut(s) 77
PdmI GAANNNNTTC 1 cut(s) 642
PfeI GAWTC 1 cut(s) 516
PkrI GCNGC 3 cut(s) 65, 114, 367
PleI GAGTC 2 cut(s) 514, 616
PpsI GAGTC 2 cut(s) 514, 616
Psp6I CCWGG 1 cut(s) 307
PspGI CCWGG 1 cut(s) 307
PspN4I GGNNCC 1 cut(s) 62
PspPI GGNCC 1 cut(s) 61
PstI CTGCAG 1 cut(s) 117
PstNI CAGNNNCTG 1 cut(s) 308
PsuI RGATCY 1 cut(s) 304
PvuII CAGCTG 1 cut(s) 260
RsaI GTAC 2 cut(s) 392, 563
RsaNI GTAC 2 cut(s) 391, 562
SacII CCGCGG 1 cut(s) 67
SaqAI TTAA 1 cut(s) 5
SatI GCNGC 3 cut(s) 64, 113, 366
Sau3AI GATC 2 cut(s) 273, 304
Sau96I GGNCC 1 cut(s) 61
SchI GAGTC 2 cut(s) 515, 617
ScrFI CCNGG 1 cut(s) 309
SfcI CTRYAG 1 cut(s) 113
Sfr303I CCGCGG 1 cut(s) 67
SgrBI CCGCGG 1 cut(s) 67
SmlI CTYRAG 3 cut(s) 37, 55, 353
SmoI CTYRAG 3 cut(s) 37, 55, 353
Sse9I AATT 5 cut(s) 25, 106, 456, 615, 658
SsiI CCGC 3 cut(s) 64, 66, 472
SspMI CTAG 2 cut(s) 165, 380
StyD4I CCNGG 1 cut(s) 307
StyI CCWWGG 3 cut(s) 369, 496, 630
TaaI ACNGT 1 cut(s) 408
TaiI ACGT 1 cut(s) 429
TaqI TCGA 1 cut(s) 276
TasI AATT 5 cut(s) 25, 106, 456, 615, 658
TatI WGTACW 1 cut(s) 390
TauI GCSGC 1 cut(s) 66
TfiI GAWTC 1 cut(s) 516
Tru1I TTAA 1 cut(s) 5
Tru9I TTAA 1 cut(s) 5
TscAI CASTG 1 cut(s) 367
TseFI GTSAC 2 cut(s) 422, 478
TseI GCWGC 2 cut(s) 112, 365
Tsp45I GTSAC 2 cut(s) 422, 478
TspDTI ATGAA 2 cut(s) 36, 517
TspRI CASTG 1 cut(s) 367
XagI CCTNNNNNAGG 1 cut(s) 13
XapI RAATTY 3 cut(s) 25, 106, 456
XcmI CCANNNNNNNNNTGG 2 cut(s) 367, 542
XmnI GAANNNNTTC 1 cut(s) 642
XspI CTAG 2 cut(s) 165, 380
ZraI GACGTC 1 cut(s) 427
Zsp2I ATGCAT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.