pycom01g19660

receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0012362
Physical Location & Seq
Forward (+)
18269741 .. 18272598
2858 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g19660.1

Sequence Viewer

Length: 2043 bp
ATGGGTTTTGCTGTTCTTCTCATCTCCTTCCTGATTTTCTTCACAAACCCCTTATGGGTTTGTGGAAACGCGGAGTTAAAAGCACTTTTGGACATGAAAGCGCTTTTGGACCCGCAAAGCTTGTATCTTTCTTCGTGGACCGTCAATGGAGACCCCTGCAGCGGGTCCTTTGAAGGAATAGGGTGCAATGAGGCGGGTCAAGTCGCTAACATTTCGCTGCAGGGAAAGGGTTTCACTGGGAAGCTATCTCCCTCCATTGCAGGGCTCAAGCATTTGACTGGCATTTACTTGCATTACAACTCTCTGCATGGAGAGATTCCCAGAGAGATTGCCAACTTGACGGAGCTGAGTGACTTGTATTTGAATGTGAATAATCTCTCTGGAGTGATCCCTCCCGAGATCGGAAATATGAGCAACTTGCAAGTTTTGCAACTTTGCTATAACCAGCTTACCGGAAACATCCCCACGCAGCTGGGATCTTTGAAAAACCTGAGCGTTCTTGCTCTGCAATCTAACCACTTAACCGGCGCAATACCGGCAAGTTTGGGGGACTTAGAAATGTTGATGAGACTGGATTTGAGTGTCAATCATCTTTTCGGTTCTATTCCCACAAAACTTGCTGATGCCCCGGTGCTTGAAGTTCTAGACGTTCGGAATAATACTCTATCCGGCAATGTACCTCTTGCTTTGAAGAGACTTAATGAAGGATTCTCTTATGAAAACAATCTGGGATTATGTGGAGCTGGATTCATGTCCTTAACCGCCTGCACTACTTCCGGTCATCCCAACGCGAGTAGACCCGAACCCTTTGGAGCTGGCGTGCCAACAAGAGACTTCCCGGAAACTGCAAATGTGCCTTTGCCATGCAACCAAACTCATTGCTCCAATTTGTCAAAATCCCATCAAGCCTCTGTTGCTGTTGTTGTTGGTGTCATTGTAGTAACTATTGCATTATCAGGTGTGGTGTTTGTATTCTTTACCCAACACCGACGCAGGAAACAGAAGCTTGGTAACTCATTTGATATCGCTGATGGCCGTTTTAGTACTGACGAGGCCAAAGCCAATGGAGTTTACAGGAAGAATGGATCTCCTCTCATCAGCCTTGAGTATGCCAACGGCTGGGATCCTTTAGCTAATGGGAGGAATTTAAGTGCGTTTGCACAAGAAGTCTTCGGTAGCTTTAGATTCAATTTGGAAGAGGTGGAGACGGCTACTCAGTATTTCTCAGAGGTGAATTTATTGGGTAAGAGTAACTTCTCTGCAACATACAAAGGAATACTACGAGACGGGTCTGCTGTTGCCATTAAGAGCATTGGCAAATCATGCTGCAAGACGGAGGAATCTGAATTCTTGAAGGGATTGAACATGTTAACCTCGTTGAGGCACGAAAATTTGGTGAAGTTGAGAGGGTTTTGTTGCTCAAAGGGCCGGGGAGAATGCTTCCTCATTTATGACTTTGTTCCTAATGGAAATTTGCTGCAGTATCTTGATGTCAAGGACGGCGACAGCCATGTTCTTGAATGGACTACTAGGGTTTGTATTGTGAAGGGCATTGCTAAAGGTCTATCTTATTTACATGGATACAAGCCAAACAAACCTCCCCTTGTTCACCAAAACATCTCGGCTGAGAAGGTGCTGATCGACCAGCGATACAACCCCTTGCTTTCCGACTCTGGCCTGCACAAGCTTCTAACCAATGATGTTGTCTTCTCAGCACTCAAGGCCAGCGCTGCAATGGGATACCTAGCTCCCGAGTACACCACCACAGGACGGTTCACAGAGAAAAGTGACGTTTATGCATTTGGGGTGCTCATTCTCCAAATCCTATCAGGGAAGCGGGAAGTCACCAACGCAATGCGCATTGGGGCTGAGTCAGGCAGGTTCCAAGATTTCATCGACCAAAACCTCAATGGAAGGTTCTTCGAATACGAAGCATCCAAGCTTGCAAAGACTGCTTTGCTTTGCACCCATGAGTCTGCGATTGAGAGACCTTCCATGGAAGAAGTTGTTCAGGAGTTGAGTAATTGTAGCAGTTGTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase_Tyr

Protein Analysis

681

Amino Acids

73.98

Weight (kDa)

6.45

Isoelectric Point (pI)

29.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 23 - 63 3.3e-07 Leucine rich repeat N-terminal domain
LRR_14 PF23598 80 - 201 1.1e-09 Leucine-rich repeat region
LRR_8 PF13855 138 - 198 2.7e-07 Leucine rich repeat
Pkinase PF00069 409 - 671 1.3e-24 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 412 - 673 4.5e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51560
fragaria_vesca FvH4_7g28070
malus_domestica MD01G1184900.v1.1 MD07G1257200.v1.1
prunus_persica Prupe.2G281400_v2.0.a1
pyrus_communis pycom01g19660 pycom07g23010 pycom07g23020
rosa_chinensis RchiOBHm_Chr1g0375021
rosa_laevigata RLG00000026697
rosa_multiflora Rmu_sc0000554.1_g000005 Rmu_sc0017099.1_g000006
rosa_roxburghii Rroxscaffold_4G00283080
rosa_rugosa Rorug01G0387500
rosa_samantha Rh1AG398100 Rh1BG362000 Rh1CG375700 Rh1DG393200
rosa_wichuraiana Rw1G035330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1859
Acc36I ACCTGC 1 cut(s) 1869
AccB7I CCANNNNNTGG 1 cut(s) 1119
AccI GTMKAC 1 cut(s) 796
AccII CGCG 2 cut(s) 71, 791
AciI CCGC 6 cut(s) 71, 113, 162, 194, 762, 1837
AclWI GGATC 5 cut(s) 382, 484, 1093, 1118, 1131
AcoI YGGCCR 1 cut(s) 1033
AcsI RAATTY 5 cut(s) 1144, 1234, 1346, 1390, 1471
AfaI GTAC 3 cut(s) 678, 1045, 1757
AfeI AGCGCT 2 cut(s) 102, 1729
AfiI CCNNNNNNNGG 8 cut(s) 55, 161, 162, 261, 401, 1119, 1380, 1770
AflIII ACRYGT 1 cut(s) 1365
AgsI TTSAA 9 cut(s) 173, 364, 484, 638, 691, 1189, 1354, 1363, 1520
AjuI GAANNNNNNNTTGG 2 cut(s) 89, 121
Alw21I GWGCWC 1 cut(s) 1812
Alw26I GTCTC 7 cut(s) 144, 562, 688, 825, 1199, 1278, 1981
AlwI GGATC 5 cut(s) 382, 484, 1093, 1118, 1131
Ama87I CYCGRG 2 cut(s) 395, 1751
Aor51HI AGCGCT 2 cut(s) 102, 1729
AoxI GGCC 5 cut(s) 1033, 1053, 1426, 1675, 1722
ApeKI GCWGC 6 cut(s) 159, 217, 469, 1326, 1477, 1730
ApoI RAATTY 5 cut(s) 1144, 1234, 1346, 1390, 1471
Asp700I GAANNNNTTC 1 cut(s) 2007
AspLEI GCGC 4 cut(s) 103, 530, 1730, 1860
AspS9I GGNCC 4 cut(s) 109, 138, 165, 1426
AsuC2I CCSGG 3 cut(s) 629, 839, 1430
AsuHPI GGTGA 4 cut(s) 1243, 1408, 1601, 1837
AsuII TTCGAA 1 cut(s) 1923
AvaI CYCGRG 2 cut(s) 395, 1751
AvaII GGWCC 3 cut(s) 109, 138, 165
BamHI GGATCC 1 cut(s) 1123
BanII GRGCYC 1 cut(s) 267
BbsI GAAGAC 2 cut(s) 1162, 1699
Bbv12I GWGCWC 1 cut(s) 1812
BbvI GCAGC 6 cut(s) 171, 204, 481, 1313, 1464, 1717
BccI CCATC 2 cut(s) 909, 1025
BceAI ACGGC 4 cut(s) 1020, 1132, 1224, 1516
BciVI GTATCC 2 cut(s) 1574, 1733
BcnI CCSGG 3 cut(s) 629, 839, 1430
BcoDI GTCTC 7 cut(s) 144, 562, 688, 825, 1199, 1278, 1981
BfaI CTAG 3 cut(s) 644, 1530, 1745
BfmI CTRYAG 3 cut(s) 157, 218, 1478
BfoI RGCGCY 2 cut(s) 104, 1731
BfuAI ACCTGC 1 cut(s) 1869
BfuI GTATCC 2 cut(s) 1574, 1733
BisI GCNGC 6 cut(s) 160, 218, 470, 1327, 1478, 1731
BlsI GCNGC 6 cut(s) 161, 219, 471, 1328, 1479, 1732
BmcAI AGTACT 1 cut(s) 1045
Bme1390I CCNGG 3 cut(s) 629, 839, 1430
Bme18I GGWCC 3 cut(s) 109, 138, 165
BmeT110I CYCGRG 2 cut(s) 395, 1751
BmgT120I GGNCC 4 cut(s) 109, 138, 165, 1426
BmiI GGNNCC 4 cut(s) 111, 166, 1125, 1883
BmrFI CCNGG 3 cut(s) 629, 839, 1430
BmrI ACTGGG 1 cut(s) 246
BmsI GCATC 2 cut(s) 613, 1943
BmuI ACTGGG 1 cut(s) 246
BpiI GAAGAC 2 cut(s) 1162, 1699
BpmI CTGGAG 1 cut(s) 402
Bpu10I CCTNAGC 1 cut(s) 491
Bpu14I TTCGAA 1 cut(s) 1923
BpuEI CTTGAG 3 cut(s) 251, 1124, 1703
BpuMI CCSGG 3 cut(s) 629, 839, 1430
BsaI GGTCTC 2 cut(s) 144, 1981
BsaJI CCNNGG 3 cut(s) 627, 1429, 1995
BsaWI WCCGGW 2 cut(s) 452, 776
BsaXI ACNNNNNCTCC 2 cut(s) 804, 834
Bsc4I CCNNNNNNNGG 8 cut(s) 55, 161, 162, 261, 401, 1119, 1380, 1770
Bse118I RCCGGY 2 cut(s) 524, 535
Bse1I ACTGG 3 cut(s) 241, 283, 576
Bse3DI GCAATG 7 cut(s) 193, 255, 679, 877, 1551, 1740, 1860
BseDI CCNNGG 3 cut(s) 627, 1429, 1995
BseGI GGATG 3 cut(s) 459, 781, 1934
BseLI CCNNNNNNNGG 8 cut(s) 55, 161, 162, 261, 401, 1119, 1380, 1770
BseMI GCAATG 7 cut(s) 193, 255, 679, 877, 1551, 1740, 1860
BseMII CTCAG 7 cut(s) 338, 482, 1229, 1239, 1617, 1725, 1860
BseNI ACTGG 3 cut(s) 241, 283, 576
BseRI GAGGAG 1 cut(s) 1080
BseXI GCAGC 6 cut(s) 171, 204, 481, 1313, 1464, 1717
BseYI CCCAGC 2 cut(s) 472, 1119
BsgI GTGCAG 2 cut(s) 751, 1664
Bsh1236I CGCG 2 cut(s) 71, 791
BshFI GGCC 5 cut(s) 1035, 1055, 1428, 1677, 1724
BsiHKAI GWGCWC 1 cut(s) 1812
BsiHKCI CYCGRG 2 cut(s) 395, 1751
BsiSI CCGG 8 cut(s) 453, 525, 536, 629, 669, 777, 839, 1429
BslFI GGGAC 1 cut(s) 563
BslI CCNNNNNNNGG 8 cut(s) 55, 161, 162, 261, 401, 1119, 1380, 1770
BsmAI GTCTC 7 cut(s) 144, 562, 688, 825, 1199, 1278, 1981
BsmBI CGTCTC 2 cut(s) 1199, 1278
BsmFI GGGAC 1 cut(s) 563
BsmI GAATGC 1 cut(s) 1442
BsnI GGCC 5 cut(s) 1035, 1055, 1428, 1677, 1724
Bso31I GGTCTC 2 cut(s) 144, 1981
BsoBI CYCGRG 2 cut(s) 395, 1751
Bsp119I TTCGAA 1 cut(s) 1923
Bsp1286I GDGCHC 2 cut(s) 267, 1812
Bsp143I GATC 6 cut(s) 387, 399, 476, 1085, 1123, 1638
Bsp19I CCATGG 1 cut(s) 1995
BspACI CCGC 6 cut(s) 71, 113, 162, 194, 762, 1837
BspANI GGCC 5 cut(s) 1035, 1055, 1428, 1677, 1724
BspCNI CTCAG 7 cut(s) 339, 483, 1228, 1238, 1618, 1724, 1861
BspFNI CGCG 2 cut(s) 71, 791
BspLI GGNNCC 4 cut(s) 111, 166, 1125, 1883
BspMAI CTGCAG 3 cut(s) 161, 222, 1482
BspMI ACCTGC 1 cut(s) 1869
BspPI GGATC 5 cut(s) 382, 484, 1093, 1118, 1131
BspT104I TTCGAA 1 cut(s) 1923
BspTNI GGTCTC 2 cut(s) 144, 1981
BsrDI GCAATG 7 cut(s) 193, 255, 679, 877, 1551, 1740, 1860
BsrFI RCCGGY 2 cut(s) 524, 535
BsrI ACTGG 3 cut(s) 241, 283, 576
BssAI RCCGGY 2 cut(s) 524, 535
BssECI CCNNGG 3 cut(s) 627, 1429, 1995
BssMI GATC 6 cut(s) 387, 399, 476, 1085, 1123, 1638
BssT1I CCWWGG 1 cut(s) 1995
Bst4CI ACNGT 2 cut(s) 142, 1773
Bst6I CTCTTC 2 cut(s) 686, 1191
BstAPI GCANNNNNTGC 3 cut(s) 427, 1323, 1952
BstBI TTCGAA 1 cut(s) 1923
BstC8I GCNNGC 6 cut(s) 766, 817, 821, 1679, 1726, 1944
BstDEI CTNAG 8 cut(s) 347, 491, 553, 1215, 1225, 1626, 1711, 1869
BstDSI CCRYGG 1 cut(s) 1995
BstENI CCTNNNNNAGG 1 cut(s) 1378
BstF5I GGATG 3 cut(s) 459, 781, 1934
BstFNI CGCG 2 cut(s) 71, 791
BstH2I RGCGCY 2 cut(s) 104, 1731
BstHHI GCGC 4 cut(s) 103, 530, 1730, 1860
BstKTI GATC 6 cut(s) 390, 402, 479, 1088, 1126, 1641
BstMAI GTCTC 7 cut(s) 144, 562, 688, 825, 1199, 1278, 1981
BstMBI GATC 6 cut(s) 387, 399, 476, 1085, 1123, 1638
BstMWI GCNNNNNNNGC 8 cut(s) 427, 536, 914, 1323, 1425, 1721, 1730, 1952
BstNSI RCATGY 1 cut(s) 1369
BstSCI CCNGG 3 cut(s) 627, 837, 1428
BstSFI CTRYAG 3 cut(s) 157, 218, 1478
BstUI CGCG 2 cut(s) 71, 791
BstV1I GCAGC 6 cut(s) 171, 204, 481, 1313, 1464, 1717
BstV2I GAAGAC 2 cut(s) 1162, 1699
BstX2I RGATCY 3 cut(s) 476, 1085, 1123
BstXI CCANNNNNNTGG 1 cut(s) 472
BstYI RGATCY 3 cut(s) 476, 1085, 1123
BsuI GTATCC 2 cut(s) 1574, 1733
BsuRI GGCC 5 cut(s) 1035, 1055, 1428, 1677, 1724
BtgI CCRYGG 1 cut(s) 1995
BtsCI GGATG 3 cut(s) 459, 781, 1934
BtsIMutI CAGTG 1 cut(s) 234
BveI ACCTGC 1 cut(s) 1869
Cac8I GCNNGC 6 cut(s) 766, 817, 821, 1679, 1726, 1944
CfoI GCGC 4 cut(s) 103, 530, 1730, 1860
Cfr10I RCCGGY 2 cut(s) 524, 535
Cfr13I GGNCC 4 cut(s) 109, 138, 165, 1426
CseI GACGC 1 cut(s) 999
Csp6I GTAC 3 cut(s) 677, 1044, 1756
CspCI CAANNNNNGTGG 2 cut(s) 598, 633
CviQI GTAC 3 cut(s) 677, 1044, 1756
DdeI CTNAG 8 cut(s) 347, 491, 553, 1215, 1225, 1626, 1711, 1869
DpnI GATC 6 cut(s) 389, 401, 478, 1087, 1125, 1640
DpnII GATC 6 cut(s) 387, 399, 476, 1085, 1123, 1638
EaeI YGGCCR 1 cut(s) 1033
Eam1104I CTCTTC 2 cut(s) 686, 1191
EarI CTCTTC 2 cut(s) 686, 1191
Eco130I CCWWGG 1 cut(s) 1995
Eco24I GRGCYC 1 cut(s) 267
Eco31I GGTCTC 2 cut(s) 144, 1981
Eco32I GATATC 1 cut(s) 1024
Eco47I GGWCC 3 cut(s) 109, 138, 165
Eco47III AGCGCT 2 cut(s) 102, 1729
Eco88I CYCGRG 2 cut(s) 395, 1751
EcoNI CCTNNNNNAGG 1 cut(s) 1378
EcoO109I RGGNCCY 1 cut(s) 165
EcoRI GAATTC 1 cut(s) 1346
EcoRV GATATC 1 cut(s) 1024
EcoT14I CCWWGG 1 cut(s) 1995
EcoT22I ATGCAT 1 cut(s) 1801
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 1995
Esp3I CGTCTC 2 cut(s) 1199, 1278
FalI AAGNNNNNCTT 2 cut(s) 1238, 1270
FaqI GGGAC 1 cut(s) 563
FauI CCCGC 4 cut(s) 120, 155, 187, 1830
FblI GTMKAC 1 cut(s) 796
Fnu4HI GCNGC 6 cut(s) 160, 218, 470, 1327, 1478, 1731
FokI GGATG 3 cut(s) 446, 768, 1921
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 6 cut(s) 160, 218, 470, 1327, 1478, 1731
FspAI RTGCGCAY 1 cut(s) 1859
FspBI CTAG 3 cut(s) 644, 1530, 1745
FspI TGCGCA 1 cut(s) 1859
GlaI GCGC 4 cut(s) 102, 529, 1729, 1859
GluI GCNGC 6 cut(s) 160, 218, 470, 1327, 1478, 1731
GsaI CCCAGC 2 cut(s) 476, 1123
GsuI CTGGAG 1 cut(s) 402
HaeII RGCGCY 2 cut(s) 104, 1731
HaeIII GGCC 5 cut(s) 1035, 1055, 1428, 1677, 1724
HapII CCGG 8 cut(s) 453, 525, 536, 629, 669, 777, 839, 1429
HgaI GACGC 1 cut(s) 999
HhaI GCGC 4 cut(s) 103, 530, 1730, 1860
Hin6I GCGC 4 cut(s) 101, 528, 1728, 1858
HinP1I GCGC 4 cut(s) 101, 528, 1728, 1858
HincII GTYRAC 1 cut(s) 1371
HindII GTYRAC 1 cut(s) 1371
HindIII AAGCTT 4 cut(s) 118, 1004, 1685, 1940
HinfI GANTC 8 cut(s) 316, 708, 747, 1185, 1340, 1670, 1871, 1973
HpaI GTTAAC 1 cut(s) 1371
HpaII CCGG 8 cut(s) 453, 525, 536, 629, 669, 777, 839, 1429
HphI GGTGA 4 cut(s) 1243, 1408, 1601, 1837
Hpy166II GTNNAC 7 cut(s) 138, 797, 1072, 1371, 1609, 1758, 1776
Hpy188I TCNGA 6 cut(s) 404, 654, 1228, 1345, 1669, 2042
Hpy188III TCNNGA 9 cut(s) 31, 381, 395, 644, 1351, 1487, 1517, 1751, 2012
Hpy8I GTNNAC 7 cut(s) 138, 797, 1072, 1371, 1609, 1758, 1776
Hpy99I CGWCG 1 cut(s) 993
HpyAV CCTTC 8 cut(s) 37, 167, 698, 1348, 1540, 1624, 1908, 2001
HpyCH4III ACNGT 2 cut(s) 142, 1773
HpyCH4IV ACGT 2 cut(s) 648, 1791
HpyF10VI GCNNNNNNNGC 8 cut(s) 427, 536, 914, 1323, 1425, 1721, 1730, 1952
HpyF3I CTNAG 8 cut(s) 347, 491, 553, 1215, 1225, 1626, 1711, 1869
HpySE526I ACGT 2 cut(s) 648, 1791
HspAI GCGC 4 cut(s) 101, 528, 1728, 1858
KspAI GTTAAC 1 cut(s) 1371
Kzo9I GATC 6 cut(s) 387, 399, 476, 1085, 1123, 1638
LmnI GCTCC 5 cut(s) 343, 740, 812, 887, 1753
Lsp1109I GCAGC 6 cut(s) 171, 204, 481, 1313, 1464, 1717
LweI GCATC 2 cut(s) 613, 1943
MaeI CTAG 3 cut(s) 644, 1530, 1745
MaeII ACGT 2 cut(s) 648, 1791
MaeIII GTNAC 6 cut(s) 350, 940, 1010, 1250, 1787, 1843
MalI GATC 6 cut(s) 389, 401, 478, 1087, 1125, 1640
MboI GATC 6 cut(s) 387, 399, 476, 1085, 1123, 1638
MflI RGATCY 3 cut(s) 476, 1085, 1123
MhlI GDGCHC 2 cut(s) 267, 1812
MluCI AATT 8 cut(s) 886, 1144, 1189, 1234, 1346, 1390, 1471, 2023
MlyI GAGTC 3 cut(s) 1664, 1880, 1982
MmeI TCCRAC 1 cut(s) 1692
Mph1103I ATGCAT 1 cut(s) 1801
MroXI GAANNNNTTC 1 cut(s) 2007
MseI TTAA 7 cut(s) 77, 521, 699, 758, 1148, 1305, 1370
MspA1I CMGCKG 2 cut(s) 162, 472
MspI CCGG 8 cut(s) 453, 525, 536, 629, 669, 777, 839, 1429
MspR9I CCNGG 3 cut(s) 629, 839, 1430
Mva1269I GAATGC 1 cut(s) 1442
MvnI CGCG 2 cut(s) 71, 791
MwoI GCNNNNNNNGC 8 cut(s) 427, 536, 914, 1323, 1425, 1721, 1730, 1952
NciI CCSGG 3 cut(s) 629, 839, 1430
NcoI CCATGG 1 cut(s) 1995
NdeII GATC 6 cut(s) 387, 399, 476, 1085, 1123, 1638
NlaIV GGNNCC 4 cut(s) 111, 166, 1125, 1883
NmeAIII GCCGAG 1 cut(s) 1601
NmuCI GTSAC 3 cut(s) 350, 1787, 1843
NsbI TGCGCA 1 cut(s) 1859
NsiI ATGCAT 1 cut(s) 1801
NspI RCATGY 1 cut(s) 1369
NspV TTCGAA 1 cut(s) 1923
PciI ACATGT 1 cut(s) 1365
PctI GAATGC 1 cut(s) 1442
PdmI GAANNNNTTC 1 cut(s) 2007
PfeI GAWTC 5 cut(s) 316, 708, 747, 1185, 1340
PflMI CCANNNNNTGG 1 cut(s) 1119
PfoI TCCNGGA 1 cut(s) 837
PkrI GCNGC 6 cut(s) 161, 219, 471, 1328, 1479, 1732
PleI GAGTC 3 cut(s) 1664, 1879, 1981
PpsI GAGTC 3 cut(s) 1664, 1879, 1981
PpuMI RGGWCCY 1 cut(s) 165
PscI ACATGT 1 cut(s) 1365
Psp5II RGGWCCY 1 cut(s) 165
PspFI CCCAGC 2 cut(s) 472, 1119
PspN4I GGNNCC 4 cut(s) 111, 166, 1125, 1883
PspPI GGNCC 4 cut(s) 109, 138, 165, 1426
PspPPI RGGWCCY 1 cut(s) 165
PstI CTGCAG 3 cut(s) 161, 222, 1482
PsuI RGATCY 3 cut(s) 476, 1085, 1123
PvuII CAGCTG 1 cut(s) 472
RsaI GTAC 3 cut(s) 678, 1045, 1757
RsaNI GTAC 3 cut(s) 677, 1044, 1756
SaqAI TTAA 7 cut(s) 77, 521, 699, 758, 1148, 1305, 1370
SatI GCNGC 6 cut(s) 160, 218, 470, 1327, 1478, 1731
Sau3AI GATC 6 cut(s) 387, 399, 476, 1085, 1123, 1638
Sau96I GGNCC 4 cut(s) 109, 138, 165, 1426
ScaI AGTACT 1 cut(s) 1045
SchI GAGTC 3 cut(s) 1664, 1880, 1982
ScrFI CCNGG 3 cut(s) 629, 839, 1430
SduI GDGCHC 2 cut(s) 267, 1812
SfaNI GCATC 2 cut(s) 613, 1943
SfcI CTRYAG 3 cut(s) 157, 218, 1478
SfuI TTCGAA 1 cut(s) 1923
SinI GGWCC 3 cut(s) 109, 138, 165
SmlI CTYRAG 3 cut(s) 266, 1103, 1718
SmoI CTYRAG 3 cut(s) 266, 1103, 1718
Sse9I AATT 8 cut(s) 886, 1144, 1189, 1234, 1346, 1390, 1471, 2023
SsiI CCGC 6 cut(s) 71, 113, 162, 194, 762, 1837
SspMI CTAG 3 cut(s) 644, 1530, 1745
StyD4I CCNGG 3 cut(s) 627, 837, 1428
StyI CCWWGG 1 cut(s) 1995
TaaI ACNGT 2 cut(s) 142, 1773
TaiI ACGT 2 cut(s) 651, 1794
TaqI TCGA 3 cut(s) 1641, 1896, 1923
TasI AATT 8 cut(s) 886, 1144, 1189, 1234, 1346, 1390, 1471, 2023
TatI WGTACW 2 cut(s) 1043, 1755
TfiI GAWTC 5 cut(s) 316, 708, 747, 1185, 1340
Tru1I TTAA 7 cut(s) 77, 521, 699, 758, 1148, 1305, 1370
Tru9I TTAA 7 cut(s) 77, 521, 699, 758, 1148, 1305, 1370
TscAI CASTG 1 cut(s) 241
TseFI GTSAC 3 cut(s) 350, 1787, 1843
TseI GCWGC 6 cut(s) 159, 217, 469, 1326, 1477, 1730
Tsp45I GTSAC 3 cut(s) 350, 1787, 1843
TspDTI ATGAA 5 cut(s) 110, 717, 732, 739, 1882
TspGWI ACGGA 2 cut(s) 356, 1349
TspRI CASTG 1 cut(s) 241
Van91I CCANNNNNTGG 1 cut(s) 1119
VpaK11BI GGWCC 3 cut(s) 109, 138, 165
XagI CCTNNNNNAGG 1 cut(s) 1378
XapI RAATTY 5 cut(s) 1144, 1234, 1346, 1390, 1471
XbaI TCTAGA 1 cut(s) 643
XceI RCATGY 1 cut(s) 1369
XcmI CCANNNNNNNNNTGG 2 cut(s) 1732, 1907
XmiI GTMKAC 1 cut(s) 796
XmnI GAANNNNTTC 1 cut(s) 2007
XspI CTAG 3 cut(s) 644, 1530, 1745
ZrmI AGTACT 1 cut(s) 1045
Zsp2I ATGCAT 1 cut(s) 1801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.