Rw1G035330

receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0012362
Physical Location & Seq
Forward (+)
63154098 .. 63157067
2970 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G035330.1

Sequence Viewer

Length: 2046 bp
ATGGCCATCACAGTTTTCCTTATCACCTTGCTATTTTTCTTCACACACCCACCGCATGTTTATGGAAACACAGAGCTGAGAGCACTCATGGACATCAAGGCCCTTTTGGACCCGCCGAGTCTGTACCTTTCTTCATGGACTGTCCATGGAGACCCGTGTGACGGGTCGTTTGAAGGTATCGGGTGCAACAAAGCGGGTCGGGTTGCCAATATTTCATTGCAAGGAAAAGGGCTATCTGGGAAGCTGTCTCCTGCCATTGGAGAGCTCAAGGATTTGACTGGGATTTACATGCATTATAACTCTTTGAAGGGAGAGATACCCAGAGAGATTGCCAACTTGACTCAGCTCAGTGACTTGTATTTGAATGTCAATAATCTAAGTGGAGAGATTCCCCCTGAGATCGGAAACGTGGGCAGCTTAGAAGTTTTGCAGCTTTGCTATAATCAGCTAACTGGAAGCATACCAACGCAGCTGGGGTCTTTGAAGAACCTTAGTGTTCTTGCTCTGCAGTCCAACCAGTTAACTGGTGCAATTCCAGCAAGTTTAGGAAACTTGGAAATGCTAATGAGACTGGATTTGAGCTACAACAACCTCTTTGGTTCTATTCCCATGAAAGTAGCTGATGCACCTTTGCTTGAAGTTCTAGACATTCGGAATAATACCCTTTCTGGCAATGTACCTCTTGTTCTGAAGAGACTAAATGATGGATTCTCTTATGAAAACAATCTGGGATTGTGTGGAGCTGGATTTATGTCCTTAACAGCCTGTAACACTTCAAGTCATCTAAATGCAAGTAGACCTGAACCCTTTGGATCAACTGGGCCAACACGAGCTATCCCGGAGACTGCAAATGTGCAGTTGCCTTGCAACCAATCTCGGTGCTCGAATATGTCAAAATCCCATCAAACATCTGTTGCTGTTGTTGTTGGTATTGTTGTACTAACAATTGCATTATCGGCTATAGGAATTCTACTCTTCACACAGCATCGTCGCCAAAAACAGAAGCTTGGGTCCTCATTTGATGTTTCTGATGCCCGCCTTAGTGTTGATGAGGCCAAGGCGAAGGGTGTAGCTCACAGGAAATATGGCTCTCCCCTTATGAGCCTTGAGTATTCTCACGGGTGGGATCCTTTAGCTGATGGTAGGAATTTAAGTGCATTTGCCCAAGACGTGTTCCATAGCTTTAAATTCAATTTGGAAGAGGTGGAGACTGCTACTCAGTATTTCTCCGAGGTGAATCTATTGGGAAAGAGTAACTTCTCTGCAACCTATAAAGGAATATTACGAGATGGGACTGTTGTGGCTGTTAAGAGCATTGGAAAAACTTGCTGCAAGACTGAGGAAGCAGAGTTCTTGAAGGGGTTGAACATGTTAACCTCACTGAGGCATGAAAATTTAGTGAAATTGAGGGGATTTTGTTGCTCAAGGGGCCGCGGAGAGTGCTTTCTCATTTATGAATTTGTTCCGAATGGAAGTTTGCTGCGATATCTTGACATCAAGGAGGGCGACAGCAATGTTCTTGAATGGTCAACTAGAGTTTCTATTGTGAAGGGCATTGCTAAAGGTCTATCTTATTTACATGGATATAAGCCAAACAAACCTGCTCTTGTTCACCAAAACATTTCGGCTGAAAAGGTGCTGATTGACCAGCGATTCAACCCCTTGCTCTCAGATTCCGGACTGCACAAGCTTCTTACCAATGATGTTGTCTTCTCAGCACTCAAGGCCAGCGCCGCTATGGGATACCTAGCTCCAGAGTACACCACTACCGGACGATTTACAGAGAAAAGTGATGTTTATGCATTTGGGGTGCTTGTTTTCCAAATTCTATCGGGGAAACGGAAGGTGACCAACTCCATGCGTCTTGGTGCTGAGTCGGGCAGATTCAAAGACTATATCGACCAAAACCTCAATGGTAGGTTCTTTGAGTATGAAGCGTCCAAGCTTGCAAAGACTGCTTTGCTTTGCACCCATGATTCTCCAATTGAGAGACCATCTATGGGAGAAGTTGTTCAAGAGTTAAGTAATTGCAGCAGCTGTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase_Tyr

Protein Analysis

681

Amino Acids

74.31

Weight (kDa)

8.06

Isoelectric Point (pI)

29.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 23 - 63 1.1e-07 Leucine rich repeat N-terminal domain
LRR_14 PF23598 80 - 218 1.2e-10 Leucine-rich repeat region
LRR_8 PF13855 139 - 198 4e-08 Leucine rich repeat
Pkinase PF00069 410 - 672 6.3e-26 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 413 - 674 2.3e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51560
fragaria_vesca FvH4_7g28070
malus_domestica MD01G1184900.v1.1 MD07G1257200.v1.1
prunus_persica Prupe.2G281400_v2.0.a1
pyrus_communis pycom01g19660 pycom07g23010 pycom07g23020
rosa_chinensis RchiOBHm_Chr1g0375021
rosa_laevigata RLG00000026697
rosa_multiflora Rmu_sc0000554.1_g000005 Rmu_sc0017099.1_g000006
rosa_roxburghii Rroxscaffold_4G00283080
rosa_rugosa Rorug01G0387500
rosa_samantha Rh1AG398100 Rh1BG362000 Rh1CG375700 Rh1DG393200
rosa_wichuraiana Rw1G035330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 297
Acc36I ACCTGC 1 cut(s) 1609
AccI GTMKAC 1 cut(s) 796
AccII CGCG 1 cut(s) 1434
AccIII TCCGGA 1 cut(s) 1676
AciI CCGC 7 cut(s) 53, 113, 194, 1036, 1432, 1434, 1734
AclWI GGATC 3 cut(s) 820, 1121, 1134
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 6 cut(s) 966, 1147, 1187, 1393, 1457, 1824
AcuI CTGAAG 1 cut(s) 710
AfaI GTAC 4 cut(s) 125, 678, 939, 1760
AfiI CCNNNNNNNGG 5 cut(s) 161, 257, 401, 1383, 2000
AflIII ACRYGT 2 cut(s) 1170, 1368
AjiI CACGTC 1 cut(s) 1171
AloI GAACNNNNNNTCC 4 cut(s) 1334, 1366, 1995, 2027
Alw21I GWGCWC 3 cut(s) 85, 267, 884
Alw26I GTCTC 7 cut(s) 144, 252, 562, 688, 836, 1202, 1984
AlwI GGATC 3 cut(s) 820, 1121, 1134
AlwNI CAGNNNCTG 1 cut(s) 2037
Aor13HI TCCGGA 1 cut(s) 1676
AoxI GGCC 6 cut(s) 3, 99, 821, 1053, 1429, 1725
ApeKI GCWGC 7 cut(s) 414, 430, 469, 1329, 1480, 2031, 2034
ApoI RAATTY 6 cut(s) 966, 1147, 1187, 1393, 1457, 1824
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
Asp700I GAANNNNTTC 2 cut(s) 1461, 2010
AspLEI GCGC 1 cut(s) 1733
AspS9I GGNCC 5 cut(s) 100, 109, 821, 1011, 1429
AsuC2I CCSGG 1 cut(s) 839
AsuHPI GGTGA 4 cut(s) 16, 1246, 1604, 1858
AvaII GGWCC 2 cut(s) 109, 1011
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 1126
BanII GRGCYC 1 cut(s) 267
BauI CACGAG 1 cut(s) 828
BbsI GAAGAC 1 cut(s) 1702
Bbv12I GWGCWC 3 cut(s) 85, 267, 884
BbvI GCAGC 5 cut(s) 426, 442, 481, 1316, 1467
BccI CCATC 6 cut(s) 14, 698, 909, 1133, 1283, 2002
BciVI GTATCC 1 cut(s) 1736
BcnI CCSGG 1 cut(s) 839
BcoDI GTCTC 7 cut(s) 144, 252, 562, 688, 836, 1202, 1984
BfaI CTAG 3 cut(s) 644, 1533, 1748
BfmI CTRYAG 2 cut(s) 506, 960
BfoI RGCGCY 1 cut(s) 1734
BfuAI ACCTGC 1 cut(s) 1609
BfuI GTATCC 1 cut(s) 1736
BisI GCNGC 9 cut(s) 415, 431, 470, 1330, 1432, 1481, 1734, 2032, 2035
BlsI GCNGC 9 cut(s) 416, 432, 471, 1331, 1433, 1482, 1735, 2033, 2036
Bme1390I CCNGG 1 cut(s) 839
Bme18I GGWCC 2 cut(s) 109, 1011
BmgBI CACGTC 1 cut(s) 1171
BmgT120I GGNCC 5 cut(s) 100, 109, 821, 1011, 1429
BmiI GGNNCC 4 cut(s) 111, 1012, 1128, 1430
BmrFI CCNGG 1 cut(s) 839
BmrI ACTGGG 2 cut(s) 288, 828
BmsI GCATC 3 cut(s) 613, 994, 1021
BmuI ACTGGG 2 cut(s) 288, 828
BpiI GAAGAC 1 cut(s) 1702
BpmI CTGGAG 1 cut(s) 1737
BpuEI CTTGAG 4 cut(s) 251, 1127, 1408, 1706
BpuMI CCSGG 1 cut(s) 839
BsaI GGTCTC 2 cut(s) 144, 1984
BsaJI CCNNGG 4 cut(s) 145, 1056, 1230, 1432
BsaWI WCCGGW 2 cut(s) 1676, 1769
BsaXI ACNNNNNCTCC 2 cut(s) 1995, 2025
Bsc4I CCNNNNNNNGG 5 cut(s) 161, 257, 401, 1383, 2000
Bse1I ACTGG 6 cut(s) 283, 457, 517, 529, 576, 823
Bse3DI GCAATG 4 cut(s) 215, 679, 1519, 1554
BseAI TCCGGA 1 cut(s) 1676
BseDI CCNNGG 4 cut(s) 145, 1056, 1230, 1432
BseLI CCNNNNNNNGG 5 cut(s) 161, 257, 401, 1383, 2000
BseMI GCAATG 4 cut(s) 215, 679, 1519, 1554
BseNI ACTGG 6 cut(s) 283, 457, 517, 529, 576, 823
BseXI GCAGC 5 cut(s) 426, 442, 481, 1316, 1467
BseYI CCCAGC 1 cut(s) 472
BsgI GTGCAG 2 cut(s) 875, 1667
Bsh1236I CGCG 1 cut(s) 1434
BshFI GGCC 6 cut(s) 5, 101, 823, 1055, 1431, 1727
BsiHKAI GWGCWC 3 cut(s) 85, 267, 884
BsiSI CCGG 3 cut(s) 839, 1677, 1770
BslFI GGGAC 1 cut(s) 1306
BslI CCNNNNNNNGG 5 cut(s) 161, 257, 401, 1383, 2000
BsmAI GTCTC 7 cut(s) 144, 252, 562, 688, 836, 1202, 1984
BsmFI GGGAC 1 cut(s) 1306
BsnI GGCC 6 cut(s) 5, 101, 823, 1055, 1431, 1727
Bso31I GGTCTC 2 cut(s) 144, 1984
Bsp1286I GDGCHC 3 cut(s) 85, 267, 884
Bsp13I TCCGGA 1 cut(s) 1676
Bsp143I GATC 3 cut(s) 399, 812, 1126
Bsp19I CCATGG 1 cut(s) 145
BspACI CCGC 7 cut(s) 53, 113, 194, 1036, 1432, 1434, 1734
BspANI GGCC 6 cut(s) 5, 101, 823, 1055, 1431, 1727
BspEI TCCGGA 1 cut(s) 1676
BspFNI CGCG 1 cut(s) 1434
BspLI GGNNCC 4 cut(s) 111, 1012, 1128, 1430
BspMAI CTGCAG 1 cut(s) 510
BspMI ACCTGC 1 cut(s) 1609
BspPI GGATC 3 cut(s) 820, 1121, 1134
BspTNI GGTCTC 2 cut(s) 144, 1984
BsrDI GCAATG 4 cut(s) 215, 679, 1519, 1554
BsrI ACTGG 6 cut(s) 283, 457, 517, 529, 576, 823
BssECI CCNNGG 4 cut(s) 145, 1056, 1230, 1432
BssMI GATC 3 cut(s) 399, 812, 1126
BssSI CACGAG 1 cut(s) 828
BssT1I CCWWGG 2 cut(s) 145, 1056
Bst2BI CACGAG 1 cut(s) 828
Bst4CI ACNGT 3 cut(s) 13, 142, 1297
Bst6I CTCTTC 3 cut(s) 686, 980, 1194
BstAPI GCANNNNNTGC 1 cut(s) 1955
BstC8I GCNNGC 3 cut(s) 1036, 1729, 1947
BstDSI CCRYGG 2 cut(s) 145, 1432
BstEII GGTNACC 1 cut(s) 1846
BstENI CCTNNNNNAGG 1 cut(s) 1381
BstFNI CGCG 1 cut(s) 1434
BstH2I RGCGCY 1 cut(s) 1734
BstHHI GCGC 1 cut(s) 1733
BstKTI GATC 3 cut(s) 402, 815, 1129
BstMAI GTCTC 7 cut(s) 144, 252, 562, 688, 836, 1202, 1984
BstMBI GATC 3 cut(s) 399, 812, 1126
BstMWI GCNNNNNNNGC 7 cut(s) 536, 956, 1428, 1440, 1724, 1733, 1955
BstNSI RCATGY 3 cut(s) 59, 292, 1372
BstPI GGTNACC 1 cut(s) 1846
BstSCI CCNGG 1 cut(s) 837
BstSFI CTRYAG 2 cut(s) 506, 960
BstUI CGCG 1 cut(s) 1434
BstV1I GCAGC 5 cut(s) 426, 442, 481, 1316, 1467
BstV2I GAAGAC 1 cut(s) 1702
BstX2I RGATCY 1 cut(s) 1126
BstXI CCANNNNNNTGG 1 cut(s) 524
BstYI RGATCY 1 cut(s) 1126
BsuI GTATCC 1 cut(s) 1736
BsuRI GGCC 6 cut(s) 5, 101, 823, 1055, 1431, 1727
BtgI CCRYGG 2 cut(s) 145, 1432
BtrI CACGTC 1 cut(s) 1171
BtsIMutI CAGTG 2 cut(s) 355, 1379
BveI ACCTGC 1 cut(s) 1609
Cac8I GCNNGC 3 cut(s) 1036, 1729, 1947
CaiI CAGNNNCTG 1 cut(s) 2037
CfoI GCGC 1 cut(s) 1733
Cfr13I GGNCC 5 cut(s) 100, 109, 821, 1011, 1429
Cfr42I CCGCGG 1 cut(s) 1435
CseI GACGC 2 cut(s) 1850, 1926
Csp6I GTAC 4 cut(s) 124, 677, 938, 1759
CviQI GTAC 4 cut(s) 124, 677, 938, 1759
DpnI GATC 3 cut(s) 401, 814, 1128
DpnII GATC 3 cut(s) 399, 812, 1126
DraI TTTAAA 1 cut(s) 1186
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 686, 980, 1194
EarI CTCTTC 3 cut(s) 686, 980, 1194
Ecl136II GAGCTC 1 cut(s) 265
Eco130I CCWWGG 2 cut(s) 145, 1056
Eco24I GRGCYC 1 cut(s) 267
Eco31I GGTCTC 2 cut(s) 144, 1984
Eco32I GATATC 1 cut(s) 1487
Eco47I GGWCC 2 cut(s) 109, 1011
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 710
Eco91I GGTNACC 1 cut(s) 1846
EcoICRI GAGCTC 1 cut(s) 265
EcoNI CCTNNNNNAGG 1 cut(s) 1381
EcoO109I RGGNCCY 2 cut(s) 100, 1011
EcoO65I GGTNACC 1 cut(s) 1846
EcoRI GAATTC 1 cut(s) 966
EcoRV GATATC 1 cut(s) 1487
EcoT14I CCWWGG 2 cut(s) 145, 1056
EcoT22I ATGCAT 2 cut(s) 294, 1804
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 2 cut(s) 145, 1056
FalI AAGNNNNNCTT 2 cut(s) 1241, 1273
FaqI GGGAC 1 cut(s) 1306
FauI CCCGC 3 cut(s) 120, 187, 1043
FblI GTMKAC 1 cut(s) 796
Fnu4HI GCNGC 9 cut(s) 415, 431, 470, 1330, 1432, 1481, 1734, 2032, 2035
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 9 cut(s) 415, 431, 470, 1330, 1432, 1481, 1734, 2032, 2035
FspBI CTAG 3 cut(s) 644, 1533, 1748
GlaI GCGC 1 cut(s) 1732
GluI GCNGC 9 cut(s) 415, 431, 470, 1330, 1432, 1481, 1734, 2032, 2035
GsaI CCCAGC 1 cut(s) 476
GsuI CTGGAG 1 cut(s) 1737
HaeII RGCGCY 1 cut(s) 1734
HaeIII GGCC 6 cut(s) 5, 101, 823, 1055, 1431, 1727
HapII CCGG 3 cut(s) 839, 1677, 1770
HgaI GACGC 2 cut(s) 1850, 1926
HhaI GCGC 1 cut(s) 1733
Hin6I GCGC 1 cut(s) 1731
HinP1I GCGC 1 cut(s) 1731
HincII GTYRAC 3 cut(s) 522, 1374, 1530
HindII GTYRAC 3 cut(s) 522, 1374, 1530
HindIII AAGCTT 3 cut(s) 1004, 1688, 1943
HpaI GTTAAC 2 cut(s) 522, 1374
HpaII CCGG 3 cut(s) 839, 1677, 1770
HphI GGTGA 4 cut(s) 16, 1246, 1604, 1858
Hpy166II GTNNAC 6 cut(s) 522, 797, 1374, 1530, 1612, 1761
Hpy188I TCNGA 8 cut(s) 404, 654, 690, 1030, 1231, 1467, 1672, 2045
Hpy188III TCNNGA 7 cut(s) 644, 1354, 1490, 1520, 1677, 1754, 2015
Hpy8I GTNNAC 6 cut(s) 522, 797, 1374, 1530, 1612, 1761
Hpy99I CGWCG 1 cut(s) 993
HpyAV CCTTC 6 cut(s) 167, 301, 1057, 1351, 1543, 1837
HpyCH4III ACNGT 3 cut(s) 13, 142, 1297
HpyCH4IV ACGT 2 cut(s) 408, 1170
HpyF10VI GCNNNNNNNGC 7 cut(s) 536, 956, 1428, 1440, 1724, 1733, 1955
HpySE526I ACGT 2 cut(s) 408, 1170
HspAI GCGC 1 cut(s) 1731
Kpn2I TCCGGA 1 cut(s) 1676
KspAI GTTAAC 2 cut(s) 522, 1374
KspI CCGCGG 1 cut(s) 1435
Kzo9I GATC 3 cut(s) 399, 812, 1126
LmnI GCTCC 2 cut(s) 740, 1756
Lsp1109I GCAGC 5 cut(s) 426, 442, 481, 1316, 1467
LweI GCATC 3 cut(s) 613, 994, 1021
MaeI CTAG 3 cut(s) 644, 1533, 1748
MaeII ACGT 2 cut(s) 408, 1170
MaeIII GTNAC 5 cut(s) 158, 350, 767, 1253, 1846
MalI GATC 3 cut(s) 401, 814, 1128
MboI GATC 3 cut(s) 399, 812, 1126
MboII GAAGA 7 cut(s) 31, 123, 496, 703, 967, 1211, 1702
MfeI CAATTG 2 cut(s) 945, 1983
MflI RGATCY 1 cut(s) 1126
MhlI GDGCHC 3 cut(s) 85, 267, 884
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 127, 334, 1883
MmeI TCCRAC 1 cut(s) 537
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 2 cut(s) 294, 1804
MroI TCCGGA 1 cut(s) 1676
MroXI GAANNNNTTC 2 cut(s) 1461, 2010
MscI TGGCCA 1 cut(s) 5
MseI TTAA 7 cut(s) 521, 758, 1151, 1185, 1308, 1373, 2021
MslI CAYNNNNRTG 2 cut(s) 60, 786
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 472, 1434, 2037
MspI CCGG 3 cut(s) 839, 1677, 1770
MspR9I CCNGG 1 cut(s) 839
MunI CAATTG 2 cut(s) 945, 1983
MvnI CGCG 1 cut(s) 1434
MwoI GCNNNNNNNGC 7 cut(s) 536, 956, 1428, 1440, 1724, 1733, 1955
NciI CCSGG 1 cut(s) 839
NcoI CCATGG 1 cut(s) 145
NdeII GATC 3 cut(s) 399, 812, 1126
NlaIV GGNNCC 4 cut(s) 111, 1012, 1128, 1430
NmeAIII GCCGAG 1 cut(s) 141
NmuCI GTSAC 3 cut(s) 158, 350, 1846
NsiI ATGCAT 2 cut(s) 294, 1804
NspI RCATGY 3 cut(s) 59, 292, 1372
PciI ACATGT 1 cut(s) 1368
PdmI GAANNNNTTC 2 cut(s) 1461, 2010
PfeI GAWTC 7 cut(s) 388, 708, 1237, 1653, 1673, 1884, 1976
PfoI TCCNGGA 1 cut(s) 837
PkrI GCNGC 9 cut(s) 416, 432, 471, 1331, 1433, 1482, 1735, 2033, 2036
PleI GAGTC 3 cut(s) 126, 334, 1882
PpsI GAGTC 3 cut(s) 126, 334, 1882
PpuMI RGGWCCY 1 cut(s) 1011
PscI ACATGT 1 cut(s) 1368
PsiI TTATAA 1 cut(s) 297
Psp124BI GAGCTC 1 cut(s) 267
Psp5II RGGWCCY 1 cut(s) 1011
PspEI GGTNACC 1 cut(s) 1846
PspFI CCCAGC 1 cut(s) 472
PspN4I GGNNCC 4 cut(s) 111, 1012, 1128, 1430
PspPI GGNCC 5 cut(s) 100, 109, 821, 1011, 1429
PspPPI RGGWCCY 1 cut(s) 1011
PsrI GAACNNNNNNTAC 2 cut(s) 669, 701
PstI CTGCAG 1 cut(s) 510
PstNI CAGNNNCTG 1 cut(s) 2037
PsuI RGATCY 1 cut(s) 1126
PvuII CAGCTG 2 cut(s) 472, 2037
RsaI GTAC 4 cut(s) 125, 678, 939, 1760
RsaNI GTAC 4 cut(s) 124, 677, 938, 1759
RseI CAYNNNNRTG 2 cut(s) 60, 786
SacI GAGCTC 1 cut(s) 267
SacII CCGCGG 1 cut(s) 1435
SaqAI TTAA 7 cut(s) 521, 758, 1151, 1185, 1308, 1373, 2021
SatI GCNGC 9 cut(s) 415, 431, 470, 1330, 1432, 1481, 1734, 2032, 2035
Sau3AI GATC 3 cut(s) 399, 812, 1126
Sau96I GGNCC 5 cut(s) 100, 109, 821, 1011, 1429
SchI GAGTC 3 cut(s) 127, 334, 1883
ScrFI CCNGG 1 cut(s) 839
SduI GDGCHC 3 cut(s) 85, 267, 884
SfaNI GCATC 3 cut(s) 613, 994, 1021
SfcI CTRYAG 2 cut(s) 506, 960
Sfr303I CCGCGG 1 cut(s) 1435
SgrBI CCGCGG 1 cut(s) 1435
SinI GGWCC 2 cut(s) 109, 1011
SmiMI CAYNNNNRTG 2 cut(s) 60, 786
SmlI CTYRAG 4 cut(s) 266, 1106, 1423, 1721
SmoI CTYRAG 4 cut(s) 266, 1106, 1423, 1721
SsiI CCGC 7 cut(s) 53, 113, 194, 1036, 1432, 1434, 1734
SspI AATATT 2 cut(s) 211, 1281
SspMI CTAG 3 cut(s) 644, 1533, 1748
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 1 cut(s) 837
StyI CCWWGG 2 cut(s) 145, 1056
TaaI ACNGT 3 cut(s) 13, 142, 1297
TaiI ACGT 2 cut(s) 411, 1173
TaqI TCGA 2 cut(s) 884, 1899
TatI WGTACW 2 cut(s) 937, 1758
TauI GCSGC 2 cut(s) 1434, 1736
TfiI GAWTC 7 cut(s) 388, 708, 1237, 1653, 1673, 1884, 1976
Tru1I TTAA 7 cut(s) 521, 758, 1151, 1185, 1308, 1373, 2021
Tru9I TTAA 7 cut(s) 521, 758, 1151, 1185, 1308, 1373, 2021
TscAI CASTG 2 cut(s) 355, 1386
TseFI GTSAC 3 cut(s) 158, 350, 1846
TseI GCWGC 7 cut(s) 414, 430, 469, 1329, 1480, 2031, 2034
Tsp45I GTSAC 3 cut(s) 158, 350, 1846
TspDTI ATGAA 7 cut(s) 123, 204, 626, 732, 1404, 1470, 1947
TspGWI ACGGA 1 cut(s) 1855
TspRI CASTG 2 cut(s) 355, 1386
VpaK11BI GGWCC 2 cut(s) 109, 1011
XagI CCTNNNNNAGG 1 cut(s) 1381
XapI RAATTY 6 cut(s) 966, 1147, 1187, 1393, 1457, 1824
XbaI TCTAGA 1 cut(s) 643
XceI RCATGY 3 cut(s) 59, 292, 1372
XcmI CCANNNNNNNNNTGG 2 cut(s) 1735, 1910
XmiI GTMKAC 1 cut(s) 796
XmnI GAANNNNTTC 2 cut(s) 1461, 2010
XspI CTAG 3 cut(s) 644, 1533, 1748
Zsp2I ATGCAT 2 cut(s) 294, 1804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.