Rh1AG398100

receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0012362
Physical Location & Seq
Forward (+)
62568661 .. 62572303
3643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG398100.1

Sequence Viewer

Length: 1488 bp
ATGCTAATGAGACTGGATTTGGGCTACAACAACCTCTTTGGTTCTATTCCCATGAAAGTAGCTGATGCACCTTTGCTTGAAGTTCTAGACATTCGGAATAATACCCTTTCTGGCAATGTACCTCTTGCTCTGAAGAGACTAAATGATGGATTCTCTTATGAAAACAATCTGGGATTGTGTGGAGCTGGATTTATGTCCTTAACAGCCTGCAACACTTCAAGTCATCTAAATGCAAGTAGACCTGAACCCTTTGGATCAACTGGGCCAACACGAGCTATCCCGGAGACTGCAAATGTGCAGTTGCCTTGCAACCAATCTCGGTGCTCGAATATGTCAAAATCCCATCAAGCATCTGTTGCTGTTGTTGTTGGTATTGTTGTACTAACAATTGCATTATCGGCTATAGGAATTCTACTCTTCACACAGCATCGTCGCCAGAAACAGAAGCTTGGGTCCTCATTTGATGTTTCTGATGCCCGCCTTAGTGTTGATGAGGCCAAGGCGAAGGGTGTAGCTCACAGGAAATATGGCTCTCCCCTTATGAGCCTCGAGTATTCTCACGGGTGGGATCCTTTAGCTGATGGGAGGAATTTAAGTGCATTTGCCCAAGACGTGTTCCATAGCTTTAAATTCAATTTGGAAGAGGTGGAGACTGCTACTCAGTATTTCTCCGAGGTGAATCTATTGGGAAAGAGTAACTTCTCTGCTACCTATAAAGGAATATTACGAGATGGGACTGTTGTGGCTGTTAAGAGCATTGGAAAAACTTGCTGCAAGACTGAGGAAGCAGAGTTCTTGAAGGGGTTGAACATGTTAACCTCACTGAGGCATGAAAATTTAGTGAAATTGAGGGGATTTTGTTGCTCAAGGGGCCGCGGAGAGTGCTTTCTCATTTATGAATTTGTTCCGAATGGAAGTTTGCTGCGATATCTTGACATCAAGGAGGGCGACAGCAATGTTCTTGAATGGTCAACTAGAGTTTCTATTGTGAAGGGCATTGCTAAAGGTCTATCTTATTTACATGGATATAAGCCAAACAAACCTGCTCTTGTTCACCAAAACATTTCGGCTGAAAAGGTGCTGATTGACCAGCGATTCAACCCCTTGCTCTCAGATTCCGGACTGCACAAGCTTCTTACCAATGATGTTGTCTTCTCAGCACTCAAGGCCAGCGCCGCTATGGGATACCTAGCTCCAGAGTACACCACTACCGGACGATTTACAGAGAAAAGTGATGTTTATGCATTTGGGGTGCTTGTTTTCCAAATTCTATCGGGGAAACGGAAGGTGACCAACTCCATGCGTCTTGGTGCTGAGTCGGGCAGATTCAAAGACTATATCGACCAAAACCTCAATGGTAGGTTCTTTGAGTATGAAGCGTCCAAGCTTGCAAAGACTGCTTTGCTTTGCACCCATGATTCTCCAATTGAGAGACCATCTATGGGAGAAGTTGTTCAAGAGTTTAGTAATTGCAGCAGCTGTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase_Tyr

Protein Analysis

495

Amino Acids

54.36

Weight (kDa)

8.78

Isoelectric Point (pI)

27.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 224 - 486 3e-26 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 227 - 487 1.6e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51560
fragaria_vesca FvH4_7g28070
malus_domestica MD01G1184900.v1.1 MD07G1257200.v1.1
prunus_persica Prupe.2G281400_v2.0.a1
pyrus_communis pycom01g19660 pycom07g23010 pycom07g23020
rosa_chinensis RchiOBHm_Chr1g0375021
rosa_laevigata RLG00000026697
rosa_multiflora Rmu_sc0000554.1_g000005 Rmu_sc0017099.1_g000006
rosa_roxburghii Rroxscaffold_4G00283080
rosa_rugosa Rorug01G0387500
rosa_samantha Rh1AG398100 Rh1BG362000 Rh1CG375700 Rh1DG393200
rosa_wichuraiana Rw1G035330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1051
AccI GTMKAC 1 cut(s) 238
AccII CGCG 1 cut(s) 876
AccIII TCCGGA 1 cut(s) 1118
AciI CCGC 4 cut(s) 478, 874, 876, 1176
AclWI GGATC 3 cut(s) 262, 563, 576
AcsI RAATTY 6 cut(s) 408, 589, 629, 835, 899, 1266
AcuI CTGAAG 1 cut(s) 152
AfaI GTAC 3 cut(s) 120, 381, 1202
AfiI CCNNNNNNNGG 2 cut(s) 825, 1442
AflIII ACRYGT 2 cut(s) 612, 810
AgsI TTSAA 9 cut(s) 80, 219, 634, 799, 808, 965, 1099, 1330, 1457
AjiI CACGTC 1 cut(s) 613
AloI GAACNNNNNNTCC 4 cut(s) 776, 808, 1437, 1469
Alw21I GWGCWC 1 cut(s) 326
Alw26I GTCTC 5 cut(s) 4, 130, 278, 644, 1426
AlwI GGATC 3 cut(s) 262, 563, 576
AlwNI CAGNNNCTG 1 cut(s) 1479
Ama87I CYCGRG 1 cut(s) 548
Aor13HI TCCGGA 1 cut(s) 1118
AoxI GGCC 4 cut(s) 263, 495, 871, 1167
ApeKI GCWGC 4 cut(s) 771, 922, 1473, 1476
ApoI RAATTY 6 cut(s) 408, 589, 629, 835, 899, 1266
Asp700I GAANNNNTTC 2 cut(s) 903, 1452
AspLEI GCGC 1 cut(s) 1175
AspS9I GGNCC 3 cut(s) 263, 453, 871
AsuC2I CCSGG 1 cut(s) 281
AsuHPI GGTGA 3 cut(s) 688, 1046, 1300
AvaI CYCGRG 1 cut(s) 548
AvaII GGWCC 1 cut(s) 453
BamHI GGATCC 1 cut(s) 568
BauI CACGAG 1 cut(s) 270
BbsI GAAGAC 1 cut(s) 1144
Bbv12I GWGCWC 1 cut(s) 326
BbvI GCAGC 2 cut(s) 758, 909
BccI CCATC 5 cut(s) 140, 351, 575, 725, 1444
BciVI GTATCC 1 cut(s) 1178
BcnI CCSGG 1 cut(s) 281
BcoDI GTCTC 5 cut(s) 4, 130, 278, 644, 1426
BfaI CTAG 3 cut(s) 86, 975, 1190
BfmI CTRYAG 1 cut(s) 402
BfoI RGCGCY 1 cut(s) 1176
BfuAI ACCTGC 1 cut(s) 1051
BfuI GTATCC 1 cut(s) 1178
BisI GCNGC 6 cut(s) 772, 874, 923, 1176, 1474, 1477
BlsI GCNGC 6 cut(s) 773, 875, 924, 1177, 1475, 1478
Bme1390I CCNGG 1 cut(s) 281
Bme18I GGWCC 1 cut(s) 453
BmeT110I CYCGRG 1 cut(s) 548
BmgBI CACGTC 1 cut(s) 613
BmgT120I GGNCC 3 cut(s) 263, 453, 871
BmiI GGNNCC 3 cut(s) 454, 570, 872
BmrFI CCNGG 1 cut(s) 281
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 4 cut(s) 55, 359, 436, 463
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 1144
BpmI CTGGAG 1 cut(s) 1179
BpuEI CTTGAG 2 cut(s) 850, 1148
BpuMI CCSGG 1 cut(s) 281
BsaI GGTCTC 1 cut(s) 1426
BsaJI CCNNGG 3 cut(s) 498, 672, 874
BsaWI WCCGGW 2 cut(s) 1118, 1211
BsaXI ACNNNNNCTCC 2 cut(s) 1437, 1467
Bsc4I CCNNNNNNNGG 2 cut(s) 825, 1442
Bse1I ACTGG 2 cut(s) 18, 265
Bse3DI GCAATG 3 cut(s) 121, 961, 996
BseAI TCCGGA 1 cut(s) 1118
BseDI CCNNGG 3 cut(s) 498, 672, 874
BseLI CCNNNNNNNGG 2 cut(s) 825, 1442
BseMI GCAATG 3 cut(s) 121, 961, 996
BseMII CTCAG 6 cut(s) 674, 771, 815, 1125, 1170, 1305
BseNI ACTGG 2 cut(s) 18, 265
BseXI GCAGC 2 cut(s) 758, 909
BsgI GTGCAG 2 cut(s) 317, 1109
Bsh1236I CGCG 1 cut(s) 876
BshFI GGCC 4 cut(s) 265, 497, 873, 1169
BsiHKAI GWGCWC 1 cut(s) 326
BsiHKCI CYCGRG 1 cut(s) 548
BsiSI CCGG 3 cut(s) 281, 1119, 1212
BslFI GGGAC 1 cut(s) 748
BslI CCNNNNNNNGG 2 cut(s) 825, 1442
BsmAI GTCTC 5 cut(s) 4, 130, 278, 644, 1426
BsmFI GGGAC 1 cut(s) 748
BsnI GGCC 4 cut(s) 265, 497, 873, 1169
Bso31I GGTCTC 1 cut(s) 1426
BsoBI CYCGRG 1 cut(s) 548
Bsp1286I GDGCHC 1 cut(s) 326
Bsp13I TCCGGA 1 cut(s) 1118
Bsp143I GATC 2 cut(s) 254, 568
BspACI CCGC 4 cut(s) 478, 874, 876, 1176
BspANI GGCC 4 cut(s) 265, 497, 873, 1169
BspCNI CTCAG 6 cut(s) 673, 772, 816, 1124, 1169, 1306
BspEI TCCGGA 1 cut(s) 1118
BspFNI CGCG 1 cut(s) 876
BspLI GGNNCC 3 cut(s) 454, 570, 872
BspMI ACCTGC 1 cut(s) 1051
BspPI GGATC 3 cut(s) 262, 563, 576
BspTNI GGTCTC 1 cut(s) 1426
BsrDI GCAATG 3 cut(s) 121, 961, 996
BsrI ACTGG 2 cut(s) 18, 265
BssECI CCNNGG 3 cut(s) 498, 672, 874
BssMI GATC 2 cut(s) 254, 568
BssSI CACGAG 1 cut(s) 270
BssT1I CCWWGG 1 cut(s) 498
Bst2BI CACGAG 1 cut(s) 270
Bst4CI ACNGT 1 cut(s) 739
Bst6I CTCTTC 3 cut(s) 128, 422, 636
BstAPI GCANNNNNTGC 2 cut(s) 356, 1397
BstC8I GCNNGC 4 cut(s) 208, 478, 1171, 1389
BstDEI CTNAG 7 cut(s) 482, 660, 780, 824, 1111, 1156, 1314
BstDSI CCRYGG 1 cut(s) 874
BstEII GGTNACC 1 cut(s) 1288
BstENI CCTNNNNNAGG 1 cut(s) 823
BstFNI CGCG 1 cut(s) 876
BstH2I RGCGCY 1 cut(s) 1176
BstHHI GCGC 1 cut(s) 1175
BstKTI GATC 2 cut(s) 257, 571
BstMAI GTCTC 5 cut(s) 4, 130, 278, 644, 1426
BstMBI GATC 2 cut(s) 254, 568
BstMWI GCNNNNNNNGC 7 cut(s) 356, 398, 870, 882, 1166, 1175, 1397
BstNSI RCATGY 1 cut(s) 814
BstPI GGTNACC 1 cut(s) 1288
BstSCI CCNGG 1 cut(s) 279
BstSFI CTRYAG 1 cut(s) 402
BstUI CGCG 1 cut(s) 876
BstV1I GCAGC 2 cut(s) 758, 909
BstV2I GAAGAC 1 cut(s) 1144
BstX2I RGATCY 1 cut(s) 568
BstYI RGATCY 1 cut(s) 568
BsuI GTATCC 1 cut(s) 1178
BsuRI GGCC 4 cut(s) 265, 497, 873, 1169
BtgI CCRYGG 1 cut(s) 874
BtrI CACGTC 1 cut(s) 613
BtsIMutI CAGTG 1 cut(s) 821
BveI ACCTGC 1 cut(s) 1051
Cac8I GCNNGC 4 cut(s) 208, 478, 1171, 1389
CaiI CAGNNNCTG 1 cut(s) 1479
CfoI GCGC 1 cut(s) 1175
Cfr13I GGNCC 3 cut(s) 263, 453, 871
Cfr42I CCGCGG 1 cut(s) 877
CseI GACGC 2 cut(s) 1292, 1368
Csp6I GTAC 3 cut(s) 119, 380, 1201
CviAII CATG 6 cut(s) 52, 811, 830, 1022, 1300, 1415
CviQI GTAC 3 cut(s) 119, 380, 1201
DdeI CTNAG 7 cut(s) 482, 660, 780, 824, 1111, 1156, 1314
DpnI GATC 2 cut(s) 256, 570
DpnII GATC 2 cut(s) 254, 568
DraI TTTAAA 1 cut(s) 628
Eam1104I CTCTTC 3 cut(s) 128, 422, 636
EarI CTCTTC 3 cut(s) 128, 422, 636
Eco130I CCWWGG 1 cut(s) 498
Eco31I GGTCTC 1 cut(s) 1426
Eco32I GATATC 1 cut(s) 929
Eco47I GGWCC 1 cut(s) 453
Eco57I CTGAAG 1 cut(s) 152
Eco88I CYCGRG 1 cut(s) 548
Eco91I GGTNACC 1 cut(s) 1288
EcoNI CCTNNNNNAGG 1 cut(s) 823
EcoO109I RGGNCCY 1 cut(s) 453
EcoO65I GGTNACC 1 cut(s) 1288
EcoRI GAATTC 1 cut(s) 408
EcoRV GATATC 1 cut(s) 929
EcoT14I CCWWGG 1 cut(s) 498
EcoT22I ATGCAT 1 cut(s) 1246
ErhI CCWWGG 1 cut(s) 498
FaeI CATG 6 cut(s) 55, 814, 833, 1025, 1303, 1418
FalI AAGNNNNNCTT 2 cut(s) 683, 715
FaqI GGGAC 1 cut(s) 748
FatI CATG 6 cut(s) 51, 810, 829, 1021, 1299, 1414
FauI CCCGC 1 cut(s) 485
FblI GTMKAC 1 cut(s) 238
Fnu4HI GCNGC 6 cut(s) 772, 874, 923, 1176, 1474, 1477
Fsp4HI GCNGC 6 cut(s) 772, 874, 923, 1176, 1474, 1477
FspBI CTAG 3 cut(s) 86, 975, 1190
GlaI GCGC 1 cut(s) 1174
GluI GCNGC 6 cut(s) 772, 874, 923, 1176, 1474, 1477
GsuI CTGGAG 1 cut(s) 1179
HaeII RGCGCY 1 cut(s) 1176
HaeIII GGCC 4 cut(s) 265, 497, 873, 1169
HapII CCGG 3 cut(s) 281, 1119, 1212
HgaI GACGC 2 cut(s) 1292, 1368
HhaI GCGC 1 cut(s) 1175
Hin1II CATG 6 cut(s) 55, 814, 833, 1025, 1303, 1418
Hin6I GCGC 1 cut(s) 1173
HinP1I GCGC 1 cut(s) 1173
HincII GTYRAC 2 cut(s) 816, 972
HindII GTYRAC 2 cut(s) 816, 972
HindIII AAGCTT 3 cut(s) 446, 1130, 1385
HinfI GANTC 7 cut(s) 150, 679, 1095, 1115, 1316, 1326, 1418
HpaI GTTAAC 1 cut(s) 816
HpaII CCGG 3 cut(s) 281, 1119, 1212
HphI GGTGA 3 cut(s) 688, 1046, 1300
Hpy166II GTNNAC 5 cut(s) 239, 816, 972, 1054, 1203
Hpy188I TCNGA 7 cut(s) 96, 132, 472, 673, 909, 1114, 1487
Hpy188III TCNNGA 7 cut(s) 86, 796, 932, 962, 1119, 1196, 1457
Hpy8I GTNNAC 5 cut(s) 239, 816, 972, 1054, 1203
Hpy99I CGWCG 1 cut(s) 435
HpyAV CCTTC 4 cut(s) 499, 793, 985, 1279
HpyCH4III ACNGT 1 cut(s) 739
HpyCH4IV ACGT 1 cut(s) 612
HpyF10VI GCNNNNNNNGC 7 cut(s) 356, 398, 870, 882, 1166, 1175, 1397
HpyF3I CTNAG 7 cut(s) 482, 660, 780, 824, 1111, 1156, 1314
HpySE526I ACGT 1 cut(s) 612
Hsp92II CATG 6 cut(s) 55, 814, 833, 1025, 1303, 1418
HspAI GCGC 1 cut(s) 1173
Kpn2I TCCGGA 1 cut(s) 1118
KspAI GTTAAC 1 cut(s) 816
KspI CCGCGG 1 cut(s) 877
Kzo9I GATC 2 cut(s) 254, 568
LmnI GCTCC 2 cut(s) 182, 1198
Lsp1109I GCAGC 2 cut(s) 758, 909
LweI GCATC 4 cut(s) 55, 359, 436, 463
MaeI CTAG 3 cut(s) 86, 975, 1190
MaeII ACGT 1 cut(s) 612
MaeIII GTNAC 2 cut(s) 695, 1288
MalI GATC 2 cut(s) 256, 570
MboI GATC 2 cut(s) 254, 568
MboII GAAGA 4 cut(s) 145, 409, 653, 1144
MfeI CAATTG 2 cut(s) 387, 1425
MflI RGATCY 1 cut(s) 568
MhlI GDGCHC 1 cut(s) 326
MlyI GAGTC 1 cut(s) 1325
Mph1103I ATGCAT 1 cut(s) 1246
MroI TCCGGA 1 cut(s) 1118
MroXI GAANNNNTTC 2 cut(s) 903, 1452
MseI TTAA 5 cut(s) 200, 593, 627, 750, 815
MslI CAYNNNNRTG 1 cut(s) 228
MspA1I CMGCKG 2 cut(s) 876, 1479
MspI CCGG 3 cut(s) 281, 1119, 1212
MspR9I CCNGG 1 cut(s) 281
MunI CAATTG 2 cut(s) 387, 1425
MvnI CGCG 1 cut(s) 876
MwoI GCNNNNNNNGC 7 cut(s) 356, 398, 870, 882, 1166, 1175, 1397
NciI CCSGG 1 cut(s) 281
NdeII GATC 2 cut(s) 254, 568
NlaIII CATG 6 cut(s) 55, 814, 833, 1025, 1303, 1418
NlaIV GGNNCC 3 cut(s) 454, 570, 872
NmuCI GTSAC 1 cut(s) 1288
NsiI ATGCAT 1 cut(s) 1246
NspI RCATGY 1 cut(s) 814
PaeR7I CTCGAG 1 cut(s) 548
PciI ACATGT 1 cut(s) 810
PdmI GAANNNNTTC 2 cut(s) 903, 1452
PfeI GAWTC 6 cut(s) 150, 679, 1095, 1115, 1326, 1418
PfoI TCCNGGA 1 cut(s) 279
PkrI GCNGC 6 cut(s) 773, 875, 924, 1177, 1475, 1478
PleI GAGTC 1 cut(s) 1324
PpsI GAGTC 1 cut(s) 1324
PpuMI RGGWCCY 1 cut(s) 453
PscI ACATGT 1 cut(s) 810
Psp5II RGGWCCY 1 cut(s) 453
PspEI GGTNACC 1 cut(s) 1288
PspN4I GGNNCC 3 cut(s) 454, 570, 872
PspPI GGNCC 3 cut(s) 263, 453, 871
PspPPI RGGWCCY 1 cut(s) 453
PspXI VCTCGAGB 1 cut(s) 548
PstNI CAGNNNCTG 1 cut(s) 1479
PsuI RGATCY 1 cut(s) 568
PvuII CAGCTG 1 cut(s) 1479
RsaI GTAC 3 cut(s) 120, 381, 1202
RsaNI GTAC 3 cut(s) 119, 380, 1201
RseI CAYNNNNRTG 1 cut(s) 228
SacII CCGCGG 1 cut(s) 877
SaqAI TTAA 5 cut(s) 200, 593, 627, 750, 815
SatI GCNGC 6 cut(s) 772, 874, 923, 1176, 1474, 1477
Sau3AI GATC 2 cut(s) 254, 568
Sau96I GGNCC 3 cut(s) 263, 453, 871
SchI GAGTC 1 cut(s) 1325
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 1 cut(s) 326
SfaNI GCATC 4 cut(s) 55, 359, 436, 463
SfcI CTRYAG 1 cut(s) 402
Sfr274I CTCGAG 1 cut(s) 548
Sfr303I CCGCGG 1 cut(s) 877
SgrBI CCGCGG 1 cut(s) 877
SinI GGWCC 1 cut(s) 453
SlaI CTCGAG 1 cut(s) 548
SmiMI CAYNNNNRTG 1 cut(s) 228
SmlI CTYRAG 3 cut(s) 548, 865, 1163
SmoI CTYRAG 3 cut(s) 548, 865, 1163
SsiI CCGC 4 cut(s) 478, 874, 876, 1176
SspI AATATT 1 cut(s) 723
SspMI CTAG 3 cut(s) 86, 975, 1190
StyD4I CCNGG 1 cut(s) 279
StyI CCWWGG 1 cut(s) 498
TaaI ACNGT 1 cut(s) 739
TaiI ACGT 1 cut(s) 615
TaqI TCGA 3 cut(s) 326, 549, 1341
TatI WGTACW 2 cut(s) 379, 1200
TauI GCSGC 2 cut(s) 876, 1178
TfiI GAWTC 6 cut(s) 150, 679, 1095, 1115, 1326, 1418
Tru1I TTAA 5 cut(s) 200, 593, 627, 750, 815
Tru9I TTAA 5 cut(s) 200, 593, 627, 750, 815
TscAI CASTG 1 cut(s) 828
TseFI GTSAC 1 cut(s) 1288
TseI GCWGC 4 cut(s) 771, 922, 1473, 1476
Tsp45I GTSAC 1 cut(s) 1288
TspDTI ATGAA 5 cut(s) 68, 174, 846, 912, 1389
TspGWI ACGGA 1 cut(s) 1297
TspRI CASTG 1 cut(s) 828
VpaK11BI GGWCC 1 cut(s) 453
XagI CCTNNNNNAGG 1 cut(s) 823
XapI RAATTY 6 cut(s) 408, 589, 629, 835, 899, 1266
XbaI TCTAGA 1 cut(s) 85
XceI RCATGY 1 cut(s) 814
XcmI CCANNNNNNNNNTGG 2 cut(s) 1177, 1352
XhoI CTCGAG 1 cut(s) 548
XmiI GTMKAC 1 cut(s) 238
XmnI GAANNNNTTC 2 cut(s) 903, 1452
XspI CTAG 3 cut(s) 86, 975, 1190
Zsp2I ATGCAT 1 cut(s) 1246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.