Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0012974

Physical Location & Seq

Genomic Coordinates Forward (+)

21555656 .. 21559926

Gene Structure 4271 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g29180.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3828 bp

ATGGCTCCCAGAAAGTTTCCACCTTTCTGGGTCTTCGCATTTGGGTGCCTTCTGATCACCACCACCACCACAGCCATTGATGACCAAAACCCAGAAGCCAATGCCCTCCTCTCCTTCAAATCCAGCCTCGAAAACCCAGAAATTCTAGCCTCATGGAAACACTCCACCCCGCTCTGCAACTGGGTCGGCGTCGCATGCCGACTCGGCCGAGTTGACTCACTCTCCCTCCCCGATTTAGGCCTCCGCGGCCCGATTCCAGCCCAGCTCGGCGGCCTGACTCAACTCACCGTCCTCGACCTCTCCTCCAACCTCCTCCGCGGCCCAATTCCGGCCCAACTCGGCGACCTGGCTCACCTCCAGACCCTCAAACTCAGCTCCAACTCGCTCGCCGGAAAAATCCCGCCGGAGATCGGAAAATTGGCGAATTTACAGACTCTTGAGCTTTCCGGAAATGGGCTCGCCGGAGAAGTCCCGGCCCAGATTGGGAACCTGACCCGGCTTCAGTACTTGGACTTGGGTAACAACCTCCTCTCTGGTTCTCTGCCTTTAGCTCTGTTTCAAAAGCTTCAGTCTTTATCTACATTGGACATATCAAACAATTCGTTTTCCGGTTTAGTTCCACCGGAAATCGGTAACCTTAGAAACCTCACTGAGCTCTACACTGGGTTGAACCGGTTTTCGGGTGCATTGCCTAAAGAAATTGGAGAGCTTTCACTGCTGGAGATTTTTTCCTCTACGGCGTGTTTGATCACCGGTCCGTTGCCGGCGGAGTTGTGGAAGCTGAAGTCACTGACCAAGCTTGATCTTTCTCATAATCCTCTGAGGTGTTCTATACCGAAAGAGATAGGAGAGTTGAAGGAGCTGAGTATATTGAATATGGTGTTTTCTGAGCTCAATGGGAGTCTCCCTCCGGAGTTGGGAAAATGTAGGAATTTGAAGACTGTGATGCTTTCGTTTAATTCGCTTTCCGGGGAGTTGCCGGAGGAGCTAAAGGAGTTGCCAGTGCTGACGTTTTCGGTTGAGAAGAACCAGCTTTCCGGGCCATTGCCGGCTTGGTTTGGGAAGTGGAGTCAGGTTGCGACGATATTGCTTTCAAGTAATCAGTTTTCGGGGAAGATTCCTCCTGAGATTGGGAATTGTTCTATGCTGATGCATCTTAGCTTGAGCAACAACTTGTTGAGGGGTGCAGTTCCAGAAGAGCTGTGCAATGCTGTTTCGCTTATGGACATTGATCTAGGTGGTAATTTCCTTTCTGGGACGATTGAGAAGACATTTGTGAGGTGTAGGAATCTTACTCAGTTGAATTTGCTGAATAATCAGATTACTGGTTCGATTCCTGAGTACCTTTCGGAGCTTCCATTGATGGCGATTCAGCTTGATTCAAACAACTTCACCGGTGAATTACCTGCTAGCCTTTGGAGCTCTGTTAATTTGATGGAGTTTTCGGCCTCTAATAATCAGTTGAATGGTTCTCTCACCAGGGAGATTGGCAATGCTGCTGCATTGGAAAGGCTTGGTCTGAGTAACAACCAGTTGGGGGGCACCATACCTAAGGAGATAGGGAATCTGAGTGCTCTCTCGGTTTTGAACCTGAACTCTAATTTCCTTGAGGGGCAGATTCCTAGTGAGGTTAGCCTCTGCAAGGCCCTCACTACACTTGACTTGGGGAGCAACAATCTCACTGGCGCGATACCGGAGGAGCTTGGTGACCTTGCTGAGTTACAGTGTCTAATCCTTTCGCGGAATGATCTGTCCGGGCCGATCCCTTCAAAGCCATCACTCTATTTTCGGCAGGTGAATTTCCCGGATATAAGTTTTGTTCAGCATGTTGGTGTATTTGATCTATCCTATAATAGATTGTCTGGCCATATACCGGAAGACTTAGGTGAATGTGTAGTTCTTGTGAATTTGGTGATCAACAACAACATGCTCTCTGGGCGAGTTCCTACATCACTCTCTCGCTTAACAAATCTCACAACCTTGGATTTGTCTGGGAATATGCTTAGTGGTTCCATTCCTCCAGAGTTTGGTGATTCCCCCAAGCTTCAAGGGCTGTATCTGGGAAACAACCAGCTTACAAGCACCATTCCTGAAAGCTTGGGTCGTTTAGGCAGTTTGGTGAAGCTGAATTTAACTGGTAATAAGTTATCTGGTGCTGTACCGGCAAGTTTTGGGAACCTGAAAGGGCTAACTCACTTAGATTTGAGTTCTAATAAGCTAGATGGTGAGCTTCCTTCTTCTCTTTCAAGCATGCAGAATCTGGGAGGGTTGTATGTGCAAGAGAACAGGCTTTCTGGTCGAATAGATGAGTTGTTCTCAAACTCTATGGCTTGGAGGATTGAAACTGTCAACTTGAGTCACAATTTCCTCACTGGGGAATTGCCCCTTTCATTGGGAAATCTTTCATACTTGACATATTTGGATCTTCATGCAAATTTGTTTGAAGGAAAGATTCCTCCAGACCTTGGGAACCTGGTGCAACTTGAGTATTTTGATGTTTCTAGTAACCAGCTATCTGGACATATTCCTGAGCAAGTGTGCAACCTCACCAGTCTGGTTTACTTGAATTTGGCCGAAAATGGTCTGGAAGGGACGATTCCCAGGAGTGGAATTTGCAGGGATCTTTCGAAAATATCTATTGTTGGTAACAAGAAATTATGTGGGAGAAGTGCGAGTTTTGATTGCCGAGTCAAAAGCTTAGACAAATCAGCACTGCTAAATGCCTGGGGACTTGCAGCAATTGTAATTGGAAGTGCTCTGATCTCTCTTGCAGGGGTCGTGGCTCTGATCAGATGGCTTACAAGAAGCAGCCGGCAAAGCGATCTTGAAGAAAGAGAGGATAGCAAGCTGAAGAGTTTCATGGATCACCATCTCTATTTCCTGAGCAATAGCCAACCAAAGGAGCCTCTGAGCATCAATATGGCCATGTTTGGGCAGCCTCTTCTGAAACTAACTCTAGCGGATATCCTTGAAGCTACCAACAATTTCGACAAGATAAATATTATTGGAGATGGGGGGTTCGGAACTGTGTACAAAGCCACTTTGTCCAACGGGAAAACAGTTGCAGTGAAGAAGCTGAGTGAGTATAAAACCCAGGGTCACCGAGAATTTATTGCTGAAATGGAAACCCTGGGTAAGGTGAAGCACCAGAATCTTGTTTCATTGCTGGGATATTGTTCTATCGGTGAGGAGAAGCTCCTTGTTTATGAATACATGGTGAATGGGAGCCTAGACCTGTGGCTGAGAAATCGGATGGGAGCTCTTGACGTCCTAGATTGGGACAGACGTTTCAAAATTGCAATGGGTGCAGCGCGAGGCCTATCATTTCTTCACCATGAATTAGTCCCCCACATCATTCACAGGGATATCAAAGCCAGCAACATCTTACTCAATGAAGACTTTGAGCCAAAGGTTGCAGACTTTGGACTTGCAAGACTGATCAGTGCCTGTGAGACTCATATCACAACTGATGTTGCTGGAACATTTGGCTACATTCCACCAGAGTATGGACAAAGTGGGAGGTCGACCAAAAAGGGAGATGTCTATAGTTTTGGTGTGATACTGCTTGAACTGGTGACTGGAAAAGAGCCAACTGGACCCGACTTCAAAGAGAAGGAAGGTGGAAATTTGGTAGGCTGGGTGCTTCAGAAGGTCAAGAAGGGGCAAGCTGCTGATGTTCTTGACCCTTTGGTACTCAGTGTGGATTCTAAACAGGCCATGTTGCAAGTGCTGCAGATTGCTTGTGTGTGCCTGTGTGACAACCCTGCTAGCAGACCTACAATGCTTCAAGTGCTGAAGATTCTTAAAGGGATGAAAGATGACGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001708 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005575 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009556 GO:0009653 GO:0009791 GO:0009908 GO:0009987 GO:0010234 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019953 GO:0022402 GO:0022414 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0034293 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0043934 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044703 GO:0045165 GO:0046777 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048656 GO:0048657 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051704 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140096 GO:1901564 GO:1903046 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1276

Amino Acids

138.33

Weight (kDa)

5.55

Isoelectric Point (pI)

32.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	30 - 66	1e-10	Leucine rich repeat N-terminal domain
LRR_14	PF23598	93 - 221	2.6e-16	Leucine-rich repeat region
LRR_8	PF13855	94 - 153	1.5e-07	Leucine rich repeat
LRR_8	PF13855	118 - 177	9.8e-06	Leucine rich repeat
LRR_8	PF13855	166 - 220	5.1e-06	Leucine rich repeat
LRR_14	PF23598	223 - 329	4.1e-07	Leucine-rich repeat region
LRR_14	PF23598	486 - 579	8.6e-07	Leucine-rich repeat region
LRR_8	PF13855	525 - 584	7.1e-06	Leucine rich repeat
LRR_8	PF13855	634 - 692	1.2e-06	Leucine rich repeat
LRR_8	PF13855	676 - 716	2.7e-06	Leucine rich repeat
LRR_8	PF13855	705 - 764	9.3e-08	Leucine rich repeat
LRR_14	PF23598	798 - 860	4.2e-06	Leucine-rich repeat region
Pkinase	PF00069	997 - 1266	6.4e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	1000 - 1267	3.3e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012974)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07280
fragaria_vesca	FvH4_7g29180
malus_domestica	MD01G1198500.v1.1 MD07G1266400.v1.1
prunus_persica	Prupe.2G292000_v2.0.a1
pyrus_communis	pycom01g20870 pycom07g24420
rosa_chinensis	RchiOBHm_Chr1g0376951
rosa_laevigata	RLG00000026557
rosa_multiflora	Rmu_sc0026264.1_g000001 Rmu_ssc0000155.1_g000035
rosa_roxburghii	Rroxscaffold_4G00281610
rosa_rugosa	Rorug01G0399800
rosa_samantha	Rh1AG417000 Rh1BG376400 Rh1CG390200 Rh1DG407100
rosa_wichuraiana	Rw1G036600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1783
AatII	GACGTC	1 cut(s)	3270
Acc36I	ACCTGC	2 cut(s)	1414, 1783
AccB1I	GGYRCC	2 cut(s)	45, 1541
AccB7I	CCANNNNNTGG	3 cut(s)	2027, 2465, 3506
AccBSI	CCGCTC	1 cut(s)	172
AccI	GTMKAC	1 cut(s)	3524
AccII	CGCG	5 cut(s)	246, 318, 1688, 1741, 3313
AccIII	TCCGGA	2 cut(s)	446, 910
AclWI	GGATC	4 cut(s)	1756, 2430, 2628, 2871
AcoI	YGGCCR	4 cut(s)	205, 1864, 2571, 2922
AcuI	CTGAAG	6 cut(s)	485, 551, 803, 2870, 3629, 3815
AcyI	GRCGYC	2 cut(s)	189, 3267
AfaI	GTAC	5 cut(s)	506, 1343, 2160, 3032, 3693
AgeI	ACCGGT	3 cut(s)	672, 752, 1394
AjnI	CCWGG	7 cut(s)	345, 1478, 2472, 2600, 2723, 3093, 3129
AloI	GAACNNNNNNTCC	2 cut(s)	1019, 1051
Alw21I	GWGCWC	6 cut(s)	657, 894, 1424, 1576, 2758, 3262
Alw26I	GTCTC	2 cut(s)	908, 3446
AlwI	GGATC	4 cut(s)	1756, 2430, 2628, 2871
AlwNI	CAGNNNCTG	2 cut(s)	2260, 3447
Aor13HI	TCCGGA	2 cut(s)	446, 910
ApeKI	GCWGC	8 cut(s)	1496, 1499, 2735, 2808, 2935, 3308, 3668, 3730
AsiGI	ACCGGT	3 cut(s)	672, 752, 1394
Asp700I	GAANNNNTTC	2 cut(s)	2401, 2855
AspLEI	GCGC	2 cut(s)	1688, 3313
AspS9I	GGNCC	9 cut(s)	248, 320, 331, 475, 755, 1040, 1645, 1757, 3596
AsuC2I	CCSGG	6 cut(s)	473, 496, 970, 1039, 1756, 1805
AsuII	TTCGAA	1 cut(s)	2627
AsuNHI	GCTAGC	2 cut(s)	1409, 3767
AvaII	GGWCC	2 cut(s)	755, 3596
AxyI	CCTNAGG	1 cut(s)	1551
BaeGI	GKGCMC	1 cut(s)	1544
BalI	TGGCCA	2 cut(s)	1866, 2924
BanI	GGYRCC	2 cut(s)	45, 1541
BanII	GRGCYC	5 cut(s)	459, 657, 894, 1424, 3262
BbsI	GAAGAC	5 cut(s)	25, 944, 1274, 1884, 3402
Bbv12I	GWGCWC	6 cut(s)	657, 894, 1424, 1576, 2758, 3262
BbvI	GCAGC	8 cut(s)	1483, 1486, 2747, 2820, 2947, 3320, 3655, 3717
BccI	CCATC	8 cut(s)	1357, 1429, 1783, 2216, 2787, 2877, 3005, 3247
BceAI	ACGGC	1 cut(s)	753
BcgI	CGANNNNNNTGC	4 cut(s)	166, 200, 1069, 1103
BciT130I	CCWGG	7 cut(s)	347, 1480, 2474, 2602, 2725, 3095, 3131
BclI	TGATCA	5 cut(s)	54, 747, 1914, 2787, 3438
BcnI	CCSGG	6 cut(s)	473, 496, 970, 1039, 1756, 1805
BcoDI	GTCTC	2 cut(s)	908, 3446
BfmI	CTRYAG	2 cut(s)	3544, 3731
BfuAI	ACCTGC	2 cut(s)	1414, 1783
BglI	GCCNNNNNGGC	2 cut(s)	204, 246
BmcAI	AGTACT	1 cut(s)	506
Bme18I	GGWCC	2 cut(s)	755, 3596
BmgT120I	GGNCC	9 cut(s)	248, 320, 331, 475, 755, 1040, 1645, 1757, 3596
BmrI	ACTGGG	3 cut(s)	190, 672, 2382
BmsI	GCATC	4 cut(s)	936, 1140, 1162, 2922
BmtI	GCTAGC	2 cut(s)	1413, 3771
BmuI	ACTGGG	3 cut(s)	190, 672, 2382
BpiI	GAAGAC	5 cut(s)	25, 944, 1274, 1884, 3402
BplI	GAGNNNNNCTC	4 cut(s)	892, 924, 2300, 2332
BpmI	CTGGAG	4 cut(s)	341, 740, 2004, 2442
Bpu10I	CCTNAGC	2 cut(s)	2529, 2882
Bpu14I	TTCGAA	1 cut(s)	2627
BpuEI	CTTGAG	5 cut(s)	458, 1183, 1628, 2374, 2504
BpuMI	CCSGG	6 cut(s)	473, 496, 970, 1039, 1756, 1805
BsaBI	GATNNNNATC	1 cut(s)	2868
BsaHI	GRCGYC	2 cut(s)	189, 3267
BsaWI	WCCGGW	9 cut(s)	446, 608, 622, 672, 752, 910, 1394, 1693, 1873
BsaXI	ACNNNNNCTCC	6 cut(s)	206, 210, 236, 240, 287, 317
Bse118I	RCCGGY	7 cut(s)	672, 752, 763, 1048, 1394, 2161, 2811
Bse21I	CCTNAGG	1 cut(s)	1551
Bse3DI	GCAATG	6 cut(s)	686, 1043, 1213, 1498, 3161, 3306
Bse8I	GATNNNNATC	1 cut(s)	2868
BseAI	TCCGGA	2 cut(s)	446, 910
BseBI	CCWGG	7 cut(s)	347, 1480, 2474, 2602, 2725, 3095, 3131
BseGI	GGATG	2 cut(s)	3258, 3816
BseJI	GATNNNNATC	1 cut(s)	2868
BseMI	GCAATG	6 cut(s)	686, 1043, 1213, 1498, 3161, 3306
BseRI	GAGGAG	7 cut(s)	98, 292, 302, 518, 998, 1712, 3203
BseSI	GKGCMC	1 cut(s)	1544
BseX3I	CGGCCG	1 cut(s)	205
BseXI	GCAGC	8 cut(s)	1483, 1486, 2747, 2820, 2947, 3320, 3655, 3717
BseYI	CCCAGC	3 cut(s)	261, 3166, 3636
BsgI	GTGCAG	2 cut(s)	1206, 3327
Bsh1236I	CGCG	5 cut(s)	246, 318, 1688, 1741, 3313
Bsh1285I	CGRYCG	1 cut(s)	208
BshNI	GGYRCC	2 cut(s)	45, 1541
BshTI	ACCGGT	3 cut(s)	672, 752, 1394
BsiEI	CGRYCG	1 cut(s)	208
BsiHKAI	GWGCWC	6 cut(s)	657, 894, 1424, 1576, 2758, 3262
BslFI	GGGAC	6 cut(s)	455, 1270, 2605, 2742, 3293, 3329
BsmAI	GTCTC	2 cut(s)	908, 3446
BsmFI	GGGAC	6 cut(s)	455, 1270, 2605, 2742, 3293, 3329
Bsp119I	TTCGAA	1 cut(s)	2627
Bsp1286I	GDGCHC	8 cut(s)	459, 657, 894, 1424, 1544, 1576, 2758, 3262
Bsp13I	TCCGGA	2 cut(s)	446, 910
Bsp1407I	TGTACA	1 cut(s)	3030
BspEI	TCCGGA	2 cut(s)	446, 910
BspFNI	CGCG	5 cut(s)	246, 318, 1688, 1741, 3313
BspMAI	CTGCAG	1 cut(s)	3735
BspMI	ACCTGC	2 cut(s)	1414, 1783
BspOI	GCTAGC	2 cut(s)	1413, 3771
BspPI	GGATC	4 cut(s)	1756, 2430, 2628, 2871
BspQI	GCTCTTC	1 cut(s)	1191
BspT104I	TTCGAA	1 cut(s)	2627
BspT107I	GGYRCC	2 cut(s)	45, 1541
BsrBI	CCGCTC	1 cut(s)	172
BsrDI	GCAATG	6 cut(s)	686, 1043, 1213, 1498, 3161, 3306
BsrFI	RCCGGY	7 cut(s)	672, 752, 763, 1048, 1394, 2161, 2811
BsrGI	TGTACA	1 cut(s)	3030
BssAI	RCCGGY	7 cut(s)	672, 752, 763, 1048, 1394, 2161, 2811
BssNI	GRCGYC	2 cut(s)	189, 3267
BssT1I	CCWWGG	2 cut(s)	1980, 2464
Bst2UI	CCWGG	7 cut(s)	347, 1480, 2474, 2602, 2725, 3095, 3131
Bst4CI	ACNGT	6 cut(s)	289, 943, 1725, 2347, 3028, 3061
Bst6I	CTCTTC	3 cut(s)	1191, 2846, 2946
BstACI	GRCGYC	2 cut(s)	189, 3267
BstAPI	GCANNNNNTGC	2 cut(s)	3305, 3730
BstAUI	TGTACA	1 cut(s)	3030
BstBI	TTCGAA	1 cut(s)	2627
BstDSI	CCRYGG	2 cut(s)	244, 316
BstEII	GGTNACC	3 cut(s)	632, 1706, 3098
BstENI	CCTNNNNNAGG	2 cut(s)	1640, 3134
BstF5I	GGATG	2 cut(s)	3258, 3816
BstFNI	CGCG	5 cut(s)	246, 318, 1688, 1741, 3313
BstHHI	GCGC	2 cut(s)	1688, 3313
BstMAI	GTCTC	2 cut(s)	908, 3446
BstMCI	CGRYCG	1 cut(s)	208
BstNI	CCWGG	7 cut(s)	347, 1480, 2474, 2602, 2725, 3095, 3131
BstNSI	RCATGY	4 cut(s)	198, 1829, 1930, 2254
BstPI	GGTNACC	3 cut(s)	632, 1706, 3098
BstSFI	CTRYAG	2 cut(s)	3544, 3731
BstSLI	GKGCMC	1 cut(s)	1544
BstUI	CGCG	5 cut(s)	246, 318, 1688, 1741, 3313
BstV1I	GCAGC	8 cut(s)	1483, 1486, 2747, 2820, 2947, 3320, 3655, 3717
BstV2I	GAAGAC	5 cut(s)	25, 944, 1274, 1884, 3402
BstX2I	RGATCY	2 cut(s)	2422, 2620
BstXI	CCANNNNNNTGG	2 cut(s)	27, 2516
BstYI	RGATCY	2 cut(s)	2422, 2620
BstZI	CGGCCG	1 cut(s)	205
Bsu36I	CCTNAGG	1 cut(s)	1551
BtgI	CCRYGG	2 cut(s)	244, 316
BtsCI	GGATG	2 cut(s)	3258, 3816
BtsI	GCAGTG	3 cut(s)	713, 2711, 3072
BveI	ACCTGC	2 cut(s)	1414, 1783
CaiI	CAGNNNCTG	2 cut(s)	2260, 3447
CfoI	GCGC	2 cut(s)	1688, 3313
Cfr10I	RCCGGY	7 cut(s)	672, 752, 763, 1048, 1394, 2161, 2811
Cfr13I	GGNCC	9 cut(s)	248, 320, 331, 475, 755, 1040, 1645, 1757, 3596
Cfr42I	CCGCGG	2 cut(s)	247, 319
CpoI	CGGWCCG	1 cut(s)	755
CseI	GACGC	1 cut(s)	178
CsiI	ACCWGGT	1 cut(s)	2472
Csp6I	GTAC	5 cut(s)	505, 1342, 2159, 3031, 3692
CspAI	ACCGGT	3 cut(s)	672, 752, 1394
CspCI	CAANNNNNGTGG	2 cut(s)	58, 93
CspI	CGGWCCG	1 cut(s)	755
CviQI	GTAC	5 cut(s)	505, 1342, 2159, 3031, 3692
EaeI	YGGCCR	4 cut(s)	205, 1864, 2571, 2922
EagI	CGGCCG	1 cut(s)	205
Eam1104I	CTCTTC	3 cut(s)	1191, 2846, 2946
EarI	CTCTTC	3 cut(s)	1191, 2846, 2946
EciI	GGCGGA	1 cut(s)	782
Ecl136II	GAGCTC	4 cut(s)	655, 892, 1422, 3260
EclXI	CGGCCG	1 cut(s)	205
Eco130I	CCWWGG	2 cut(s)	1980, 2464
Eco147I	AGGCCT	2 cut(s)	240, 3318
Eco24I	GRGCYC	5 cut(s)	459, 657, 894, 1424, 3262
Eco32I	GATATC	2 cut(s)	2965, 3367
Eco47I	GGWCC	2 cut(s)	755, 3596
Eco52I	CGGCCG	1 cut(s)	205
Eco53kI	GAGCTC	4 cut(s)	655, 892, 1422, 3260
Eco57I	CTGAAG	6 cut(s)	485, 551, 803, 2870, 3629, 3815
Eco81I	CCTNAGG	1 cut(s)	1551
Eco91I	GGTNACC	3 cut(s)	632, 1706, 3098
EcoICRI	GAGCTC	4 cut(s)	655, 892, 1422, 3260
EcoNI	CCTNNNNNAGG	2 cut(s)	1640, 3134
EcoO109I	RGGNCCY	1 cut(s)	1645
EcoO65I	GGTNACC	3 cut(s)	632, 1706, 3098
EcoRII	CCWGG	7 cut(s)	345, 1478, 2472, 2600, 2723, 3093, 3129
EcoRV	GATATC	2 cut(s)	2965, 3367
EcoT14I	CCWWGG	2 cut(s)	1980, 2464
EcoT22I	ATGCAT	1 cut(s)	1155
EcoT38I	GRGCYC	5 cut(s)	459, 657, 894, 1424, 3262
ErhI	CCWWGG	2 cut(s)	1980, 2464
FaqI	GGGAC	6 cut(s)	455, 1270, 2605, 2742, 3293, 3329
FauI	CCCGC	2 cut(s)	177, 408
FbaI	TGATCA	5 cut(s)	54, 747, 1914, 2787, 3438
FblI	GTMKAC	1 cut(s)	3524
FokI	GGATG	2 cut(s)	3265, 3823
FriOI	GRGCYC	5 cut(s)	459, 657, 894, 1424, 3262
GlaI	GCGC	2 cut(s)	1687, 3312
GsaI	CCCAGC	3 cut(s)	265, 3170, 3640
GsuI	CTGGAG	4 cut(s)	341, 740, 2004, 2442
HgaI	GACGC	1 cut(s)	178
HhaI	GCGC	2 cut(s)	1688, 3313
Hin1I	GRCGYC	2 cut(s)	189, 3267
Hin6I	GCGC	2 cut(s)	1686, 3311
HinP1I	GCGC	2 cut(s)	1686, 3311
HincII	GTYRAC	3 cut(s)	214, 2350, 3525
HindII	GTYRAC	3 cut(s)	214, 2350, 3525
HindIII	AAGCTT	5 cut(s)	563, 797, 2042, 2095, 2695
Hpy166II	GTNNAC	5 cut(s)	214, 2350, 2560, 3031, 3525
Hpy8I	GTNNAC	5 cut(s)	214, 2350, 2560, 3031, 3525
Hpy99I	CGWCG	2 cut(s)	194, 1084
HpyCH4III	ACNGT	6 cut(s)	289, 943, 1725, 2347, 3028, 3061
HpyCH4IV	ACGT	3 cut(s)	1010, 3267, 3286
HpySE526I	ACGT	3 cut(s)	1010, 3267, 3286
Hsp92I	GRCGYC	2 cut(s)	189, 3267
HspAI	GCGC	2 cut(s)	1686, 3311
Kpn2I	TCCGGA	2 cut(s)	446, 910
KroI	GCCGGC	3 cut(s)	763, 1048, 2811
KroNI	GCCGGC	3 cut(s)	765, 1050, 2813
Ksp22I	TGATCA	5 cut(s)	54, 747, 1914, 2787, 3438
KspI	CCGCGG	2 cut(s)	247, 319
LguI	GCTCTTC	1 cut(s)	1191
Lsp1109I	GCAGC	8 cut(s)	1483, 1486, 2747, 2820, 2947, 3320, 3655, 3717
LweI	GCATC	4 cut(s)	936, 1140, 1162, 2922
MabI	ACCWGGT	1 cut(s)	2472
MaeII	ACGT	3 cut(s)	1010, 3267, 3286
MbiI	CCGCTC	1 cut(s)	172
MfeI	CAATTG	1 cut(s)	2739
MflI	RGATCY	2 cut(s)	2422, 2620
MhlI	GDGCHC	8 cut(s)	459, 657, 894, 1424, 1544, 1576, 2758, 3262
MlsI	TGGCCA	2 cut(s)	1866, 2924
MluNI	TGGCCA	2 cut(s)	1866, 2924
MlyI	GAGTC	9 cut(s)	195, 209, 271, 427, 910, 1078, 2365, 2697, 3448
MmeI	TCCRAC	3 cut(s)	330, 402, 3072
Mox20I	TGGCCA	2 cut(s)	1866, 2924
Mph1103I	ATGCAT	1 cut(s)	1155
MroI	TCCGGA	2 cut(s)	446, 910
MroNI	GCCGGC	3 cut(s)	763, 1048, 2811
MroXI	GAANNNNTTC	2 cut(s)	2401, 2855
MscI	TGGCCA	2 cut(s)	1866, 2924
MseI	TTAA	5 cut(s)	957, 1428, 1964, 2132, 3804
MslI	CAYNNNNRTG	3 cut(s)	43, 1830, 2918
Msp20I	TGGCCA	2 cut(s)	1866, 2924
MspA1I	CMGCKG	2 cut(s)	246, 318
MunI	CAATTG	1 cut(s)	2739
MvaI	CCWGG	7 cut(s)	347, 1480, 2474, 2602, 2725, 3095, 3131
MvnI	CGCG	5 cut(s)	246, 318, 1688, 1741, 3313
NaeI	GCCGGC	3 cut(s)	765, 1050, 2813
NciI	CCSGG	6 cut(s)	473, 496, 970, 1039, 1756, 1805
NgoMIV	GCCGGC	3 cut(s)	763, 1048, 2811
NheI	GCTAGC	2 cut(s)	1409, 3767
NmeAIII	GCCGAG	5 cut(s)	183, 233, 246, 318, 2711
NmuCI	GTSAC	6 cut(s)	786, 1706, 2357, 3098, 3574, 3755
NsiI	ATGCAT	1 cut(s)	1155
NspI	RCATGY	4 cut(s)	198, 1829, 1930, 2254
NspV	TTCGAA	1 cut(s)	2627
PaeI	GCATGC	2 cut(s)	198, 2254
PaqCI	CACCTGC	1 cut(s)	1783
PasI	CCCWGGG	2 cut(s)	3094, 3130
PceI	AGGCCT	2 cut(s)	240, 3318
PciSI	GCTCTTC	1 cut(s)	1191
PdiI	GCCGGC	3 cut(s)	765, 1050, 2813
PdmI	GAANNNNTTC	2 cut(s)	2401, 2855
PflMI	CCANNNNNTGG	3 cut(s)	2027, 2465, 3506
PfoI	TCCNGGA	1 cut(s)	1803
PinAI	ACCGGT	3 cut(s)	672, 752, 1394
PleI	GAGTC	9 cut(s)	195, 209, 271, 427, 909, 1077, 2364, 2696, 3448
PpsI	GAGTC	9 cut(s)	195, 209, 271, 427, 909, 1077, 2364, 2696, 3448
Psp124BI	GAGCTC	4 cut(s)	657, 894, 1424, 3262
Psp6I	CCWGG	7 cut(s)	345, 1478, 2472, 2600, 2723, 3093, 3129
PspEI	GGTNACC	3 cut(s)	632, 1706, 3098
PspFI	CCCAGC	3 cut(s)	261, 3166, 3636
PspGI	CCWGG	7 cut(s)	345, 1478, 2472, 2600, 2723, 3093, 3129
PspPI	GGNCC	9 cut(s)	248, 320, 331, 475, 755, 1040, 1645, 1757, 3596
PstI	CTGCAG	1 cut(s)	3735
PstNI	CAGNNNCTG	2 cut(s)	2260, 3447
PsuI	RGATCY	2 cut(s)	2422, 2620
RsaI	GTAC	5 cut(s)	506, 1343, 2160, 3032, 3693
RsaNI	GTAC	5 cut(s)	505, 1342, 2159, 3031, 3692
RseI	CAYNNNNRTG	3 cut(s)	43, 1830, 2918
Rsr2I	CGGWCCG	1 cut(s)	755
RsrII	CGGWCCG	1 cut(s)	755
SacI	GAGCTC	4 cut(s)	657, 894, 1424, 3262
SacII	CCGCGG	2 cut(s)	247, 319
SalI	GTCGAC	1 cut(s)	3523
SapI	GCTCTTC	1 cut(s)	1191
SaqAI	TTAA	5 cut(s)	957, 1428, 1964, 2132, 3804
Sau96I	GGNCC	9 cut(s)	248, 320, 331, 475, 755, 1040, 1645, 1757, 3596
ScaI	AGTACT	1 cut(s)	506
SchI	GAGTC	9 cut(s)	195, 209, 271, 427, 910, 1078, 2365, 2697, 3448
SduI	GDGCHC	8 cut(s)	459, 657, 894, 1424, 1544, 1576, 2758, 3262
SexAI	ACCWGGT	1 cut(s)	2472
SfaNI	GCATC	4 cut(s)	936, 1140, 1162, 2922
SfcI	CTRYAG	2 cut(s)	3544, 3731
SfiI	GGCCNNNNNGGCC	1 cut(s)	246
Sfr303I	CCGCGG	2 cut(s)	247, 319
SfuI	TTCGAA	1 cut(s)	2627
SgrAI	CRCCGGYG	1 cut(s)	1394
SgrBI	CCGCGG	2 cut(s)	247, 319
SinI	GGWCC	2 cut(s)	755, 3596
SmiMI	CAYNNNNRTG	3 cut(s)	43, 1830, 2918
SmlI	CTYRAG	5 cut(s)	437, 1162, 1607, 2353, 2483
SmoI	CTYRAG	5 cut(s)	437, 1162, 1607, 2353, 2483
SphI	GCATGC	2 cut(s)	198, 2254
SseBI	AGGCCT	2 cut(s)	240, 3318
SspI	AATATT	1 cut(s)	3001
SstI	GAGCTC	4 cut(s)	657, 894, 1424, 3262
StuI	AGGCCT	2 cut(s)	240, 3318
StyI	CCWWGG	2 cut(s)	1980, 2464
TaaI	ACNGT	6 cut(s)	289, 943, 1725, 2347, 3028, 3061
TaiI	ACGT	3 cut(s)	1013, 3270, 3289
TaqI	TCGA	7 cut(s)	129, 294, 1331, 2299, 2627, 2988, 3524
TatI	WGTACW	2 cut(s)	504, 3030
TauI	GCSGC	3 cut(s)	249, 273, 321
Tru1I	TTAA	5 cut(s)	957, 1428, 1964, 2132, 3804
Tru9I	TTAA	5 cut(s)	957, 1428, 1964, 2132, 3804
TseFI	GTSAC	6 cut(s)	786, 1706, 2357, 3098, 3574, 3755
TseI	GCWGC	8 cut(s)	1496, 1499, 2735, 2808, 2935, 3308, 3668, 3730
Tsp45I	GTSAC	6 cut(s)	786, 1706, 2357, 3098, 3574, 3755
TspDTI	ATGAA	9 cut(s)	2379, 2394, 2417, 2848, 3150, 3222, 3351, 3408, 3827
TspGWI	ACGGA	1 cut(s)	747
Van91I	CCANNNNNTGG	3 cut(s)	2027, 2465, 3506
VpaK11BI	GGWCC	2 cut(s)	755, 3596
XagI	CCTNNNNNAGG	2 cut(s)	1640, 3134
XceI	RCATGY	4 cut(s)	198, 1829, 1930, 2254
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1050
XmiI	GTMKAC	1 cut(s)	3524
XmnI	GAANNNNTTC	2 cut(s)	2401, 2855
ZraI	GACGTC	1 cut(s)	3268
ZrmI	AGTACT	1 cut(s)	506
Zsp2I	ATGCAT	1 cut(s)	1155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.