Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0012974

Physical Location & Seq

Genomic Coordinates Forward (+)

19167964 .. 19171871

Gene Structure 3908 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom01g20870.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3768 bp

ATGGCGTCGAAAAAGCTTCTGTTTTTAGTAATTTTCTTAGCTTTTGAGTTTCTCTTTCTACTCGCCAATGGAGTTTCCCACCAAAACGACCAAAACCAAGGAATGGAAGCCCTTCTTTCCTTCAAAACCGCCCTCGGAAACCCCGCAGTTCTCTCCTCGTGGCGGCCCTCCGCCTCGCACTGCGACTGGGTCGGCGTTTCCTGCCAATTTGGCCGAGTCACCGCCCTCGCCCTTCCGTCCCTCTCCCTCAAAGGCCCTCTACCTCCCTCCCTCTTCTCCCTTTCAAACCTTGTCGTGGTGGACCTCTCCTCCAACCTACTCCGCGGCAAAATCCCACCTCAAGTTCTCAACCTCAAACGCCTAAGACACCTCTGCCTCACCGATAACCAGCTCTCCGGCGAAATACTCACCGAACTCGCCGAGTTAACTCAGCTAGAGACTTTGAAGCTCGGAACCAACTTTTTCGCCGGAAAAATCCCGCCAGAACTTGGAAGTTTAGCACTGCTTCAAACCTTGGAGCTCTCCAACAATGGCTTCCATGGAGATATTCCGACCCAGATTGGAAACTTGACCCGGCTGCAGTTCCTGGACCTGGGCAACAATGTCCTCTCAGGTTCTCTTCCCTCTGCCCTGTTTCCAAAACTTCAATCTTTAGCTTCCATGGACGTTTCAAACAATTCATTTTCCGGCGCAATTCCACCTGAGATTGGTAGCCTGAAGAATCTCACCGAGCTCTACGTTGGCATCAACCATTTCTCCGGCCCGTTACCCCGCGAAATCGGTGAGCTTTCGCGGCTAGAAATTCTGTACGCTGCTTCCTGTTCCATAACAGGGCCGTTGCCGGAAGAGATTTCTAAGCTGGAATCTTTGACCAATATTGACCTCTCGTACAACCCATTGAGGTGTTCGTTGCCAAAAGGCATTGGGAAGTTGCAGAAGCTGACTATTTTGGATTTGGTCTTCACTGAGCTCAACGGGTCTGTTCCTGCTGAGCTGGGGAACTGTAAAAGTTTGAGGACTTTGATGCTTTCGTTCAATTCGCTTTCTGGGACGTTGCCTGAGGAGCTCGCTGAGCTTCCGGTGATGACGTTTTCTGCCGAAAAGAACCAGCTCTCCGGCCTGTTGCCTTCCTGGCTTGGAAAGTTGAAGCAAGTCGAATCGATTCTGCTTTCCAGCAATCTATTTTCCGGGAAAATTCCACCGGAGATTGGCAACTGCTCTATGCTGAGGTCACTTAGTTTGAGCAATAACCTGTTGACGGGTCCGATTCCGGAAGAGCTTTGCAATGCGGGTTCGCTTTCGGAGATTGATCTCGATAGCAATTTCCTCTCGGGAACAATTGACAACACGTTTGTCAATTGCAAGAACTTGACGCAGCTCAACTTGGTGAACAATCAGATCGCCGGCGCAATCCCTGACTACCTCTCGGAGCTTCCTCTTTGGAATTCGGAAAATTTGATGGAGTTCACAGCTTCGAACAATCAGCTGCGCGGTCCGATATCGAATGAGATTGGTCGGGCAGCCGTCCTGGAAAGGCTTGTCCTTAGTAACAATCAGCTGAGAGGCACTCTTCCTAAAGAAATTGGAAATGTCAGCAGCCTTTCTGTTCTAATATTGAGTTCGAATCTCCTGGAAGGGAACATTCCGGCGGAGCTTGGCCAGTGCACCGGTCTTACTACATTGGAGCTCGGAAACAACCAGCTCAGCGGGTCTATCCCGGCGGAGCTCGCGGAGCTAAGCCAATTGCAATGTCTGGTACTTTCTCACAACAATCTATCAGGTTCAATTCCTTCGAAAAAATCGTTGTACTTTCGACAAGCAAACATCCCGGATCTGAGCTTTGTGCAACATGTTGGAGTGTTCGATCTGTCCTACAATAGATTGTCGGGTGCAATACCGGAAGATTTGGGCGACTGTGTTGTTCTGGTGGATGTTTTGATTAGCAACAACCTGCTCTCCGGTGAAATTCCAAGGTCCCTCGCTCGTTTAAGAAATCTTACAACTTTGGATTTGTCTGGGAACATGCTCAGTGGTTCAATTCCTCCTGAGTTTGGTGATTCGCCGAAGCTTCAAGGCTTGAATCTGGGAAATAACCAGCTTACGAGCACCATCCCCGGAAGCTTAGGTCGTTTGGGAAGCTTGGTGAAGCTTAATTTGACTGGTAACAAGTTGTCCGGTGCTGTACCTTTGAGCTTTGGGGACTTGAATGAGCTTACTCACTTGGATTTAAGCTCGAACGAGCTTGATGGCGAGCTTCCCTCGTCGCTGTCAGGCATGCAGAATCTTGTGGGGCTGTATGTTCAGGAGAACGGGCTTAATGGGGGTGTTGATAAGCTGTTCTCGAATAGCATGGCTTGGAGGATCGAAAAGATGAACTTGAGCAATAATCTCTTTGATGGGGAGCTGCCCCATTCTCTTGGTAATCTGTCATACTTGACGGATTTGGATCTTCATTCAAATTCGTTTCGAGGAGAGATTCCTCCAGACCTGGGGAATCTGATGCAACTTGAGTTTTTTGATGTTTCAAATAACAAGCTCTCTGGAAAGATCCCAGATAAAATTTGCAGCCTCATCAATCTGTTTTACGTGAACTTGGCGGAAAACAGGTTGGAAGGGCCGATTCCTAAGAGTGGTATCTGCCGGAACCTGTCAGAAAAATCGCTTGCCGGTAACAAGAAATTATGTGGGAGAAGTAAAAATTTGGATTGCCATGCCGAGAGTTTTGATAAATCAGCACTGTTGAATGCCTGGGGAGTTGCTGCTGTTGTAGTTGGAAGTGCTCTAATCCTTATTGTTGCAGCTTATGCTGTAGTAAGATGGATCACCCGAAGCAGCCGGCGTGATCCTGAAGAAGTTGAAGAACGCAGGTTGAGCAGTTTCATGGAACATAATCTCTATTTTCTCAGTAGCAGCAGCAGATCAAAGGAGCCTCTGAGCATCAACGTTGCCATGTTTCAGCAGCCGCTACTCAAGCTTACTTTAGTTGACATTCTTGAAGCCACCAACAACTTCTGCAAGACAAACATAATAGGAGACGGAGGCTTTGGAACTGTGTACAAGGCCACATTGTCGGATGGGAAAACAGTCGCGGTTAAGAAGCTAAGCGAGTATAAAACTCAGGGCCATCGAGAATTCATCGCTGAAATGGAAACCCTGGGGAAGGTAAACCACCAGAATCTCGTTCCATTACTCGGGTACTGCTCTCTTGGCGAGGAGAAGCTCCTTGTTTACGAGTATATGGTAAATGGGAGCCTGGACATTTGGCTGAGAAATCGGACTGGGGATCTTGAAGTCCTAGATTGGGACAGACGTTTCAAAATCGCAATGGGTGCAGCCCGCGGCCTGGCTTTTCTTCACCATGGATTCAGCCCCCACATCATCCACAGGGACATCAAAGCCAGCAACATCTTGCTCAACGAAGACTTCGAGCCCAAAGTTGCAGACTTTGGACTCGCAAGCGGGCGGTCTACCACAAAAGGAGATGTCTATAGTTTTGGCGTGATACTGCTCGAACTGGTGACAGGGAAAGAGCCAACCGGACCCGATTTCAAAGACCTGGAAGGCGGGAATTTAGTCGGGTGGGTTTTTCAGATGATGAAGAAGGGGAAGGCAGCTGATGTTCTTGATCCGGTAGTACTCGACGCGGATTCGAAGCGCAGGATGGTTCAAGTGTTGAAGATTGCCTGTGTGTGCCTCTCTGATAACCCAGCTCAGAGGCCTACGATGCTTCAAGTGTTGAAGTTTCTCAAAGGGATTAGTGATGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001708 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005575 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009556 GO:0009653 GO:0009791 GO:0009908 GO:0009987 GO:0010234 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019953 GO:0022402 GO:0022414 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0034293 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0043934 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044703 GO:0045165 GO:0046777 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048656 GO:0048657 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051704 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140096 GO:1901564 GO:1903046 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1256

Amino Acids

136.28

Weight (kDa)

5.48

Isoelectric Point (pI)

32.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	36 - 68	1.6e-09	Leucine rich repeat N-terminal domain
LRR_14	PF23598	158 - 249	3.2e-09	Leucine-rich repeat region
LRR_14	PF23598	247 - 356	3e-11	Leucine-rich repeat region
LRR_4	PF12799	665 - 702	2.3e-06	Leucine Rich repeats (2 copies)
LRR_8	PF13855	684 - 724	1.3e-06	Leucine rich repeat
LRR_8	PF13855	716 - 772	5.2e-06	Leucine rich repeat
Pkinase	PF00069	1007 - 1153	4.3e-28	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	1008 - 1153	2.4e-31	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012974)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07280
fragaria_vesca	FvH4_7g29180
malus_domestica	MD01G1198500.v1.1 MD07G1266400.v1.1
prunus_persica	Prupe.2G292000_v2.0.a1
pyrus_communis	pycom01g20870 pycom07g24420
rosa_chinensis	RchiOBHm_Chr1g0376951
rosa_laevigata	RLG00000026557
rosa_multiflora	Rmu_sc0026264.1_g000001 Rmu_ssc0000155.1_g000035
rosa_roxburghii	Rroxscaffold_4G00281610
rosa_rugosa	Rorug01G0399800
rosa_samantha	Rh1AG417000 Rh1BG376400 Rh1CG390200 Rh1DG407100
rosa_wichuraiana	Rw1G036600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1959, 2856
AccB7I	CCANNNNNTGG	2 cut(s)	103, 488
AccI	GTMKAC	1 cut(s)	3467
AccII	CGCG	8 cut(s)	324, 774, 793, 1491, 1730, 3089, 3339, 3644
AccIII	TCCGGA	1 cut(s)	1270
AclI	AACGTT	1 cut(s)	2943
AclWI	GGATC	8 cut(s)	1839, 2369, 2454, 2542, 2828, 2837, 3291, 3620
AcoI	YGGCCR	2 cut(s)	211, 1657
AcuI	CTGAAG	2 cut(s)	737, 2868
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	8 cut(s)	809, 890, 1758, 1808, 2184, 3056, 3197, 3636
AflIII	ACRYGT	2 cut(s)	1347, 1849
AgeI	ACCGGT	1 cut(s)	1667
AjuI	GAANNNNNNNTTGG	2 cut(s)	518, 550
AloI	GAACNNNNNNTCC	2 cut(s)	1097, 1129
Alw21I	GWGCWC	9 cut(s)	522, 735, 972, 1068, 1667, 1689, 1728, 2108, 2782
Alw26I	GTCTC	2 cut(s)	431, 3027
Alw44I	GTGCAC	1 cut(s)	1663
AlwI	GGATC	8 cut(s)	1839, 2369, 2454, 2542, 2828, 2837, 3291, 3620
AlwNI	CAGNNNCTG	2 cut(s)	586, 940
Ama87I	CYCGRG	2 cut(s)	1330, 3191
Aor13HI	TCCGGA	1 cut(s)	1270
ApaLI	GTGCAC	1 cut(s)	1663
ArsI	GACNNNNNNTTYG	4 cut(s)	1334, 1366, 3026, 3058
AsiGI	ACCGGT	1 cut(s)	1667
Asp700I	GAANNNNTTC	2 cut(s)	111, 1161
AspLEI	GCGC	4 cut(s)	692, 1409, 1491, 3657
AsuC2I	CCSGG	5 cut(s)	574, 1189, 1718, 1829, 2115
AsuII	TTCGAA	4 cut(s)	1475, 1622, 1793, 3650
AvaI	CYCGRG	2 cut(s)	1330, 3191
AvaII	GGWCC	6 cut(s)	301, 589, 1262, 1493, 1974, 3539
AxyI	CCTNAGG	1 cut(s)	1059
BaeGI	GKGCMC	1 cut(s)	1667
BalI	TGGCCA	1 cut(s)	1659
BanII	GRGCYC	7 cut(s)	522, 735, 972, 1068, 1689, 1728, 3432
BauI	CACGAG	1 cut(s)	157
BbsI	GAAGAC	2 cut(s)	952, 3426
Bbv12I	GWGCWC	9 cut(s)	522, 735, 972, 1068, 1667, 1689, 1728, 2108, 2782
BbvCI	CCTCAGC	1 cut(s)	1226
BccI	CCATC	8 cut(s)	1453, 2117, 2240, 2390, 2811, 3068, 3132, 3655
BceAI	ACGGC	2 cut(s)	820, 1508
BcnI	CCSGG	5 cut(s)	574, 1189, 1718, 1829, 2115
BcoDI	GTCTC	2 cut(s)	431, 3027
BfaI	CTAG	3 cut(s)	434, 797, 3296
BfmI	CTRYAG	3 cut(s)	578, 2808, 3487
BfuAI	ACCTGC	2 cut(s)	1959, 2856
BglI	GCCNNNNNGGC	2 cut(s)	210, 1132
BlpI	GCTNAGC	5 cut(s)	990, 1071, 1703, 1736, 3101
BmcAI	AGTACT	1 cut(s)	3636
Bme18I	GGWCC	6 cut(s)	301, 589, 1262, 1493, 1974, 3539
BmeT110I	CYCGRG	2 cut(s)	1330, 3191
BmiI	GGNNCC	7 cut(s)	454, 1263, 1976, 2645, 2928, 3251, 3541
BmrI	ACTGGG	2 cut(s)	196, 3288
BmsI	GCATC	5 cut(s)	753, 1014, 2490, 2946, 3714
BmuI	ACTGGG	2 cut(s)	196, 3288
BpiI	GAAGAC	2 cut(s)	952, 3426
BplI	GAGNNNNNCTC	4 cut(s)	1552, 1584, 2243, 2275
BpmI	CTGGAG	1 cut(s)	2466
Bpu10I	CCTNAGC	2 cut(s)	1226, 2122
Bpu1102I	GCTNAGC	5 cut(s)	990, 1071, 1703, 1736, 3101
Bpu14I	TTCGAA	4 cut(s)	1475, 1622, 1793, 3650
BpuEI	CTTGAG	4 cut(s)	324, 2398, 2528, 2954
BpuMI	CCSGG	5 cut(s)	574, 1189, 1718, 1829, 2115
Bsa29I	ATCGAT	1 cut(s)	1160
BsaAI	YACGTR	1 cut(s)	2587
BsaBI	GATNNNNATC	1 cut(s)	2445
BsaHI	GRCGYC	1 cut(s)	5
BsaWI	WCCGGW	9 cut(s)	1078, 1201, 1270, 1667, 1897, 1958, 2174, 3536, 3628
Bse118I	RCCGGY	4 cut(s)	1403, 1667, 2666, 2835
Bse1I	ACTGG	5 cut(s)	191, 1660, 2164, 3283, 3519
Bse21I	CCTNAGG	1 cut(s)	1059
Bse3DI	GCAATG	3 cut(s)	1291, 1754, 3330
Bse8I	GATNNNNATC	1 cut(s)	2445
BseAI	TCCGGA	1 cut(s)	1270
BseCI	ATCGAT	1 cut(s)	1160
BseGI	GGATG	6 cut(s)	1824, 1936, 2109, 3079, 3378, 3666
BseJI	GATNNNNATC	1 cut(s)	2445
BseMI	GCAATG	3 cut(s)	1291, 1754, 3330
BseNI	ACTGG	5 cut(s)	191, 1660, 2164, 3283, 3519
BseRI	GAGGAG	5 cut(s)	145, 298, 1076, 2484, 3227
BseSI	GKGCMC	1 cut(s)	1667
BseYI	CCCAGC	2 cut(s)	994, 3706
BsgI	GTGCAG	1 cut(s)	3351
Bsh1236I	CGCG	8 cut(s)	324, 774, 793, 1491, 1730, 3089, 3339, 3644
BshTI	ACCGGT	1 cut(s)	1667
BshVI	ATCGAT	1 cut(s)	1160
BsiHKAI	GWGCWC	9 cut(s)	522, 735, 972, 1068, 1667, 1689, 1728, 2108, 2782
BsiHKCI	CYCGRG	2 cut(s)	1330, 3191
BslFI	GGGAC	6 cut(s)	223, 1063, 1960, 2213, 3317, 3401
BsmAI	GTCTC	2 cut(s)	431, 3027
BsmBI	CGTCTC	1 cut(s)	3027
BsmFI	GGGAC	6 cut(s)	223, 1063, 1960, 2213, 3317, 3401
BsmI	GAATGC	1 cut(s)	2749
BsoBI	CYCGRG	2 cut(s)	1330, 3191
Bsp119I	TTCGAA	4 cut(s)	1475, 1622, 1793, 3650
Bsp13I	TCCGGA	1 cut(s)	1270
Bsp1407I	TGTACA	1 cut(s)	3054
Bsp1720I	GCTNAGC	5 cut(s)	990, 1071, 1703, 1736, 3101
Bsp19I	CCATGG	3 cut(s)	538, 660, 3358
BspDI	ATCGAT	1 cut(s)	1160
BspEI	TCCGGA	1 cut(s)	1270
BspFNI	CGCG	8 cut(s)	324, 774, 793, 1491, 1730, 3089, 3339, 3644
BspLI	GGNNCC	7 cut(s)	454, 1263, 1976, 2645, 2928, 3251, 3541
BspMAI	CTGCAG	1 cut(s)	582
BspMI	ACCTGC	2 cut(s)	1959, 2856
BspPI	GGATC	8 cut(s)	1839, 2369, 2454, 2542, 2828, 2837, 3291, 3620
BspQI	GCTCTTC	1 cut(s)	1269
BspT104I	TTCGAA	4 cut(s)	1475, 1622, 1793, 3650
BsrDI	GCAATG	3 cut(s)	1291, 1754, 3330
BsrFI	RCCGGY	4 cut(s)	1403, 1667, 2666, 2835
BsrGI	TGTACA	1 cut(s)	3054
BsrI	ACTGG	5 cut(s)	191, 1660, 2164, 3283, 3519
BssAI	RCCGGY	4 cut(s)	1403, 1667, 2666, 2835
BssNI	GRCGYC	1 cut(s)	5
BssSI	CACGAG	1 cut(s)	157
BssT1I	CCWWGG	6 cut(s)	97, 513, 538, 660, 1970, 3358
Bst2BI	CACGAG	1 cut(s)	157
Bst4CI	ACNGT	5 cut(s)	1004, 1916, 2739, 3052, 3085
Bst6I	CTCTTC	5 cut(s)	278, 624, 840, 1269, 1575
BstACI	GRCGYC	1 cut(s)	5
BstAPI	GCANNNNNTGC	2 cut(s)	2804, 3329
BstAUI	TGTACA	1 cut(s)	3054
BstBAI	YACGTR	1 cut(s)	2587
BstBI	TTCGAA	4 cut(s)	1475, 1622, 1793, 3650
BstDSI	CCRYGG	5 cut(s)	322, 538, 660, 3337, 3358
BstENI	CCTNNNNNAGG	1 cut(s)	3158
BstF5I	GGATG	6 cut(s)	1824, 1936, 2109, 3079, 3378, 3666
BstFNI	CGCG	8 cut(s)	324, 774, 793, 1491, 1730, 3089, 3339, 3644
BstHHI	GCGC	4 cut(s)	692, 1409, 1491, 3657
BstMAI	GTCTC	2 cut(s)	431, 3027
BstNSI	RCATGY	3 cut(s)	1853, 2026, 2278
BstSFI	CTRYAG	3 cut(s)	578, 2808, 3487
BstSLI	GKGCMC	1 cut(s)	1667
BstUI	CGCG	8 cut(s)	324, 774, 793, 1491, 1730, 3089, 3339, 3644
BstV2I	GAAGAC	2 cut(s)	952, 3426
BstX2I	RGATCY	4 cut(s)	1831, 2446, 2547, 3283
BstXI	CCANNNNNNTGG	1 cut(s)	2417
BstYI	RGATCY	4 cut(s)	1831, 2446, 2547, 3283
Bsu15I	ATCGAT	1 cut(s)	1160
Bsu36I	CCTNAGG	1 cut(s)	1059
BsuTUI	ATCGAT	1 cut(s)	1160
BtgI	CCRYGG	5 cut(s)	322, 538, 660, 3337, 3358
BtgZI	GCGATG	1 cut(s)	3121
BtsCI	GGATG	6 cut(s)	1824, 1936, 2109, 3079, 3378, 3666
BtsI	GCAGTG	2 cut(s)	178, 500
BtsIMutI	CAGTG	6 cut(s)	178, 500, 963, 1667, 2035, 2735
BveI	ACCTGC	2 cut(s)	1959, 2856
CaiI	CAGNNNCTG	2 cut(s)	586, 940
CfoI	GCGC	4 cut(s)	692, 1409, 1491, 3657
Cfr10I	RCCGGY	4 cut(s)	1403, 1667, 2666, 2835
Cfr42I	CCGCGG	2 cut(s)	325, 3340
ClaI	ATCGAT	1 cut(s)	1160
CpoI	CGGWCCG	1 cut(s)	1493
CseI	GACGC	2 cut(s)	1381, 3650
Csp6I	GTAC	8 cut(s)	808, 889, 1757, 1807, 2183, 3055, 3196, 3635
CspAI	ACCGGT	1 cut(s)	1667
CspI	CGGWCCG	1 cut(s)	1493
CviQI	GTAC	8 cut(s)	808, 889, 1757, 1807, 2183, 3055, 3196, 3635
EaeI	YGGCCR	2 cut(s)	211, 1657
Eam1104I	CTCTTC	5 cut(s)	278, 624, 840, 1269, 1575
EarI	CTCTTC	5 cut(s)	278, 624, 840, 1269, 1575
EciI	GGCGGA	4 cut(s)	160, 1664, 1736, 2612
Ecl136II	GAGCTC	6 cut(s)	520, 733, 970, 1066, 1687, 1726
Eco130I	CCWWGG	6 cut(s)	97, 513, 538, 660, 1970, 3358
Eco147I	AGGCCT	1 cut(s)	3718
Eco24I	GRGCYC	7 cut(s)	522, 735, 972, 1068, 1689, 1728, 3432
Eco32I	GATATC	1 cut(s)	1500
Eco47I	GGWCC	6 cut(s)	301, 589, 1262, 1493, 1974, 3539
Eco53kI	GAGCTC	6 cut(s)	520, 733, 970, 1066, 1687, 1726
Eco57I	CTGAAG	2 cut(s)	737, 2868
Eco81I	CCTNAGG	1 cut(s)	1059
Eco88I	CYCGRG	2 cut(s)	1330, 3191
EcoICRI	GAGCTC	6 cut(s)	520, 733, 970, 1066, 1687, 1726
EcoNI	CCTNNNNNAGG	1 cut(s)	3158
EcoO109I	RGGNCCY	2 cut(s)	254, 1974
EcoRI	GAATTC	2 cut(s)	1444, 3131
EcoRV	GATATC	1 cut(s)	1500
EcoT14I	CCWWGG	6 cut(s)	97, 513, 538, 660, 1970, 3358
EcoT38I	GRGCYC	7 cut(s)	522, 735, 972, 1068, 1689, 1728, 3432
ErhI	CCWWGG	6 cut(s)	97, 513, 538, 660, 1970, 3358
Esp3I	CGTCTC	1 cut(s)	3027
FalI	AAGNNNNNCTT	4 cut(s)	99, 131, 2360, 2392
FaqI	GGGAC	6 cut(s)	223, 1063, 1960, 2213, 3317, 3401
FauI	CCCGC	8 cut(s)	151, 486, 779, 1282, 1700, 3344, 3452, 3557
FblI	GTMKAC	1 cut(s)	3467
FokI	GGATG	6 cut(s)	1811, 1943, 2096, 3086, 3365, 3673
FriOI	GRGCYC	7 cut(s)	522, 735, 972, 1068, 1689, 1728, 3432
FspBI	CTAG	3 cut(s)	434, 797, 3296
GlaI	GCGC	4 cut(s)	691, 1408, 1490, 3656
GsaI	CCCAGC	2 cut(s)	998, 3710
GsuI	CTGGAG	1 cut(s)	2466
HgaI	GACGC	2 cut(s)	1381, 3650
HhaI	GCGC	4 cut(s)	692, 1409, 1491, 3657
Hin1I	GRCGYC	1 cut(s)	5
Hin6I	GCGC	4 cut(s)	690, 1407, 1489, 3655
HinP1I	GCGC	4 cut(s)	690, 1407, 1489, 3655
HincII	GTYRAC	3 cut(s)	426, 1257, 2986
HindII	GTYRAC	3 cut(s)	426, 1257, 2986
HindIII	AAGCTT	6 cut(s)	14, 2066, 2119, 2137, 2147, 2972
HpaI	GTTAAC	1 cut(s)	426
Hpy99I	CGWCG	3 cut(s)	10, 2266, 3644
HpyCH4III	ACNGT	5 cut(s)	1004, 1916, 2739, 3052, 3085
HpyCH4IV	ACGT	8 cut(s)	666, 738, 1052, 1088, 1349, 2586, 2943, 3310
HpySE526I	ACGT	8 cut(s)	666, 738, 1052, 1088, 1349, 2586, 2943, 3310
Hsp92I	GRCGYC	1 cut(s)	5
HspAI	GCGC	4 cut(s)	690, 1407, 1489, 3655
Kpn2I	TCCGGA	1 cut(s)	1270
KroI	GCCGGC	2 cut(s)	1403, 2835
KroNI	GCCGGC	2 cut(s)	1405, 2837
KspAI	GTTAAC	1 cut(s)	426
KspI	CCGCGG	2 cut(s)	325, 3340
LguI	GCTCTTC	1 cut(s)	1269
LweI	GCATC	5 cut(s)	753, 1014, 2490, 2946, 3714
MaeI	CTAG	3 cut(s)	434, 797, 3296
MaeII	ACGT	8 cut(s)	666, 738, 1052, 1088, 1349, 2586, 2943, 3310
MaeIII	GTNAC	7 cut(s)	217, 765, 1230, 1547, 2162, 2669, 3517
MfeI	CAATTG	3 cut(s)	1338, 1357, 1742
MflI	RGATCY	4 cut(s)	1831, 2446, 2547, 3283
MlsI	TGGCCA	1 cut(s)	1659
MluNI	TGGCCA	1 cut(s)	1659
MlyI	GAGTC	2 cut(s)	225, 3444
MmeI	TCCRAC	7 cut(s)	336, 549, 575, 1834, 2589, 2752, 3051
Mox20I	TGGCCA	1 cut(s)	1659
MreI	CGCCGGCG	1 cut(s)	1403
MroI	TCCGGA	1 cut(s)	1270
MroNI	GCCGGC	2 cut(s)	1403, 2835
MroXI	GAANNNNTTC	2 cut(s)	111, 1161
MscI	TGGCCA	1 cut(s)	1659
MseI	TTAA	6 cut(s)	425, 1988, 2151, 2228, 2316, 3093
MslI	CAYNNNNRTG	1 cut(s)	901
Msp20I	TGGCCA	1 cut(s)	1659
MspA1I	CMGCKG	6 cut(s)	324, 1486, 1558, 1707, 3339, 3614
MunI	CAATTG	3 cut(s)	1338, 1357, 1742
Mva1269I	GAATGC	1 cut(s)	2749
MvnI	CGCG	8 cut(s)	324, 774, 793, 1491, 1730, 3089, 3339, 3644
NaeI	GCCGGC	2 cut(s)	1405, 2837
NciI	CCSGG	5 cut(s)	574, 1189, 1718, 1829, 2115
NcoI	CCATGG	3 cut(s)	538, 660, 3358
NgoMIV	GCCGGC	2 cut(s)	1403, 2835
NlaIV	GGNNCC	7 cut(s)	454, 1263, 1976, 2645, 2928, 3251, 3541
NmeAIII	GCCGAG	3 cut(s)	239, 445, 2740
NmuCI	GTSAC	3 cut(s)	217, 1230, 3517
NspI	RCATGY	3 cut(s)	1853, 2026, 2278
NspV	TTCGAA	4 cut(s)	1475, 1622, 1793, 3650
PaeI	GCATGC	1 cut(s)	2278
PasI	CCCWGGG	1 cut(s)	3154
PceI	AGGCCT	1 cut(s)	3718
PciI	ACATGT	1 cut(s)	1849
PciSI	GCTCTTC	1 cut(s)	1269
PcsI	WCGNNNNNNNCGW	2 cut(s)	1799, 3423
PctI	GAATGC	1 cut(s)	2749
PdiI	GCCGGC	2 cut(s)	1405, 2837
PdmI	GAANNNNTTC	2 cut(s)	111, 1161
PflFI	GACNNNGTC	1 cut(s)	188
PflMI	CCANNNNNTGG	2 cut(s)	103, 488
PfoI	TCCNGGA	5 cut(s)	585, 1187, 1527, 1629, 1827
PinAI	ACCGGT	1 cut(s)	1667
PleI	GAGTC	2 cut(s)	224, 3444
PpsI	GAGTC	2 cut(s)	224, 3444
Ppu21I	YACGTR	1 cut(s)	2587
PpuMI	RGGWCCY	1 cut(s)	1974
PscI	ACATGT	1 cut(s)	1849
Psp124BI	GAGCTC	6 cut(s)	522, 735, 972, 1068, 1689, 1728
Psp1406I	AACGTT	1 cut(s)	2943
Psp5II	RGGWCCY	1 cut(s)	1974
PspFI	CCCAGC	2 cut(s)	994, 3706
PspN4I	GGNNCC	7 cut(s)	454, 1263, 1976, 2645, 2928, 3251, 3541
PspPPI	RGGWCCY	1 cut(s)	1974
PstI	CTGCAG	1 cut(s)	582
PstNI	CAGNNNCTG	2 cut(s)	586, 940
PsuI	RGATCY	4 cut(s)	1831, 2446, 2547, 3283
PsyI	GACNNNGTC	1 cut(s)	188
PvuII	CAGCTG	3 cut(s)	1486, 1558, 3614
RsaI	GTAC	8 cut(s)	809, 890, 1758, 1808, 2184, 3056, 3197, 3636
RsaNI	GTAC	8 cut(s)	808, 889, 1757, 1807, 2183, 3055, 3196, 3635
RseI	CAYNNNNRTG	1 cut(s)	901
Rsr2I	CGGWCCG	1 cut(s)	1493
RsrII	CGGWCCG	1 cut(s)	1493
SacI	GAGCTC	6 cut(s)	522, 735, 972, 1068, 1689, 1728
SacII	CCGCGG	2 cut(s)	325, 3340
SapI	GCTCTTC	1 cut(s)	1269
SaqAI	TTAA	6 cut(s)	425, 1988, 2151, 2228, 2316, 3093
ScaI	AGTACT	1 cut(s)	3636
SchI	GAGTC	2 cut(s)	225, 3444
SfaNI	GCATC	5 cut(s)	753, 1014, 2490, 2946, 3714
SfcI	CTRYAG	3 cut(s)	578, 2808, 3487
Sfr303I	CCGCGG	2 cut(s)	325, 3340
SfuI	TTCGAA	4 cut(s)	1475, 1622, 1793, 3650
SgrAI	CRCCGGYG	1 cut(s)	1403
SgrBI	CCGCGG	2 cut(s)	325, 3340
SinI	GGWCC	6 cut(s)	301, 589, 1262, 1493, 1974, 3539
SmiMI	CAYNNNNRTG	1 cut(s)	901
SmlI	CTYRAG	4 cut(s)	339, 2377, 2507, 2969
SmoI	CTYRAG	4 cut(s)	339, 2377, 2507, 2969
SphI	GCATGC	1 cut(s)	2278
SseBI	AGGCCT	1 cut(s)	3718
SspI	AATATT	2 cut(s)	877, 1614
SspMI	CTAG	3 cut(s)	434, 797, 3296
SstI	GAGCTC	6 cut(s)	522, 735, 972, 1068, 1689, 1728
StuI	AGGCCT	1 cut(s)	3718
StyI	CCWWGG	6 cut(s)	97, 513, 538, 660, 1970, 3358
TaaI	ACNGT	5 cut(s)	1004, 1916, 2739, 3052, 3085
TaiI	ACGT	8 cut(s)	669, 741, 1055, 1091, 1352, 2589, 2946, 3313
TatI	WGTACW	3 cut(s)	1806, 3054, 3634
TauI	GCSGC	5 cut(s)	166, 327, 796, 2965, 3342
Tru1I	TTAA	6 cut(s)	425, 1988, 2151, 2228, 2316, 3093
Tru9I	TTAA	6 cut(s)	425, 1988, 2151, 2228, 2316, 3093
TscAI	CASTG	6 cut(s)	185, 507, 970, 1667, 2035, 2742
TseFI	GTSAC	3 cut(s)	217, 1230, 3517
Tsp45I	GTSAC	3 cut(s)	217, 1230, 3517
TspDTI	ATGAA	6 cut(s)	669, 2387, 2441, 2869, 3124, 3611
TspGWI	ACGGA	3 cut(s)	225, 2453, 3051
TspRI	CASTG	6 cut(s)	185, 507, 970, 1667, 2035, 2742
Tth111I	GACNNNGTC	1 cut(s)	188
Van91I	CCANNNNNTGG	2 cut(s)	103, 488
VneI	GTGCAC	1 cut(s)	1663
VpaK11BI	GGWCC	6 cut(s)	301, 589, 1262, 1493, 1974, 3539
XagI	CCTNNNNNAGG	1 cut(s)	3158
XceI	RCATGY	3 cut(s)	1853, 2026, 2278
XmiI	GTMKAC	1 cut(s)	3467
XmnI	GAANNNNTTC	2 cut(s)	111, 1161
XspI	CTAG	3 cut(s)	434, 797, 3296
ZrmI	AGTACT	1 cut(s)	3636

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.