Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0012974

Physical Location & Seq

Genomic Coordinates Forward (+)

29626796 .. 29630698

Gene Structure 3903 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1198500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3903 bp

ATGGCGTCGAAAAAGCCTCTGTTTATAGTAATTTTCTTAGCTTTTGAGTTTCTCTTTCTACTCGCCAATGGAGTTTCCCACCAAAATGACCAAAACCCAGAAATGGAAGCCCTTCTTTCCTTCAAAACCGCCCTCGGAAACCCCACAGTTCTCTCCTCGTGGCGGCCCTCCGCCTCGCACTGCGACTGGGTCGGCGTTTCCTGCCAATTTGGCCGAGTCACCGCCCTCGCCCTTCCGTTCCTCTCCCTCAAAGGCCCTCTCCCTTCCTCCCTCTTCTCCCTTGCAAACCTTGTCGTGCTAGACCTCTCCTCCAACCTACTAAGCGGCAAAATCCCACGTCAAGTTCTCAACCTCAAACGCCTAAGCCACCTCTGCCTCACCGATAACCAGCTCTCCGGCGAAATACCCACCCAACTCGCCGAGTTAACTCAGCTAGAGACTCTGAAGCTCGGAACCAACTTTTTCGCCGGAAAAATCCCGCCAGAACTTGGAAATTTAGCACTGCTTCAAACCTTGGAGCTCTCCAACAATGGGTTCCATGGAGATATTCCTACCCAGATTGGAAACTTGACCCGGCTGCAGTTCCTGGACCTGGGCAACAATGTCCTCTCGGGTTCTCTTCCCTCTGCCCTGTTTCCAAAACTTCAATCTTTAGCTTCCATGGACGTTTCAAACAATTCATTTTCCGGCGCAATTCCACCTGAGATTGGTAGCCTGAAGGATCTCACCGAGCTCTACGTTGGCATCAACCATTTCTCCGGCCCGTTACCCCGCGAAATCGGTGAGCTTTCGCGGCTCGAAATTCTGTACGCTGCTTCCTGTTCCATAACAGGGCCGTTGCCGGAAGAGATTTCTAAGCTGGAATCGTTGACCAATATTGACCTCTCGTACAACCCGTTGAGGTGTTCGTTGCCAAAAGGCATTGGGAAGTTGCAGAAGCTGAGTATTTTGGATTTGGTCTTCACTGAGCTCAATGGCTCTATTCCTGCTGAGCTGGGGAACTGTAAAAGTTTGAGGACTTTGATGCTTTCGTTCAATTCGCTTTCGGGGACGTTGCCTGAGGAGCTCTCTGAGCTTCCGGTGATGACGTTTTCTGCCGAAAAGAACCAGCTCTCCGGCCTATTGCCTTCCTGGCTCGGAAAGTTGAAGCAAGTCGAATCGATTCTGCTTTCCAGCAATCTATTTTCCGGGAAGATTCCACCGGAGATTGGCAACTGCTCTATGCTGAGGTTACTTAGCTTGAGCAATAACATGTTGACGGGGCCGATTCCGGAAGAGCTTTGCAATGCGGGTTCGCTTTCGGAGATTGATCTCGATAGCAATTTTCTCTCGGGAACAATTGACAACACGTTTGTCAAGTGCAAGAACTTGACGCAACTCAACCTGGTGAATAATCAGATTGCCGGCGCAATCCCTGACTACCTTTCGGAGCTTCCACTCATGGTGATCGACCTTGATTCCAACAACTTCACGGGCACGATACCGAAAAGTCTTTGGAATTCGGAAAATTTGATGGAGTTCACAGCTTCGAACAATCAGCTGCGAGGTCCGATATCGAATGAGATTGGTCGGGCAGCCGTCTTGGAAAGGCTTGTTCTTAGTAACAATCAGCTGACAGGCACTCTTCCTACAGAGATTAGAAATGTCAGCAGCCTTTCTGTTCTAATATTGAGTTCGAATCTTCTGGAAGGGAACATTCCGCCTGAGCTTGGCCGGTGCACCGGTCTTACTACATTGGAGCTCGGAAACAACCTGCTCAGCGGGTCGATCCCGGCGGAGCTTGCGCAGCTGAGCCAATTGCAATGTCTGGTACTTTCTCACAACAATCTATCAGGTTCAATTCCTTCGAAAAAATCGTTGTACTTTCGACAAGTAAACATCCCGGATTTGAGCTTTGTGCAACATGTTGGAGTGTTTGATCTGTCCTACAATAGATTGTCCGGTGCAATACCGGAAGATTTGGGCGACTGTGTTGTTTTGGTGGATGTTTTGATCAGCAACAACCTGATCTCCGGTGAAATTCCAAGGTCCCTTGCTCGTTTAAGAAATCTTACAACTTTGGATTTGTCTGGGAACATGCTCAGTGGTTCAATTCCTCCAGAGTTTGGCGGTTCGCCGAAGCTTCAAGGCTTGAATCTGGCAAATAACCAGCTTACGAGCACCATACCGGGAAGCTTAGGTCGTTTGGGAAGCTTGGTGAAGCTTAATTTGACCGGTAATAAGTTGTCTGGTGCCATACCTGTGAGCTTTGGGGATTTGAAAGAGCTTACTCACTTGGATTTAAGCTCGAACGAGCTTGATGGCGAGCTTCCTTCATCGCTGTCAGGCATGCAGAATCTTGTGGGGTTGTATGTTCAGGACAACCGGCTTAATGGTGGTGTGGATAAGCTGTTCTCGAATACCATGGCTTGGAGGATCGAAAAGATGAACTTGAGCAATAATCTCTTTGATGGGGAGCTGCCCCATTCTCTTGGTAATCTGTCATACTTGACGGATTTGGATCTTCATTCAAATTCGTTTCGAGGAGAGATTCCTCCAGACCTGGGGAATCTGATGCAACTCGAGTTTTTTGATGTTTCAAATAACAAGCTCTCTGGAAGGATTCCAGATAAAATTTGCAGCCTCATCAATCTGTTTAAGGTGAACTTGGCAGAAAATAGGTTGGAAGGGCCGATTCCTAAGAGTGGTATCTGCCGGAACCTGTCAGAAAAATCGCTTGCCGGTAACAAGAAATTATGTGGGAGAAGTAAAAATTTGGATTGCCATGCCAAGAGTTTTGATAAATCAGCATTGTTGAATGCCTGGGGAGTTGCTGCTGTTGTAGTTGGAAGTGCTCTAGTCTTTATTGTTGCAGCTTATGCTGTAGTAAGATGGATCGCCCGAAGCAGCCGGCATGATCCCGAAGAAGCTGAAGAAAGCAGGTTGAGCAGTTTCATGGAACATAATCTCTATTTTCTCAGTAGCAGCAGCAGATCAAAGGAGCCTCTGAGCATCAACGTCGCCATGTTTCAGCAGCCGCTGCTCAAGCTTACTTTAGTTGACATTCTTGAAGCCACCAACAACTTCTGCAAGACAAACATAATAGGAGACGGAGGCTTTGGAACTGTGTACAAGGCCACATTGTCGGATGGGAAAACAGTCGCGGTTAAGAAGCTAAGCGAGTATAAAACTCAGGGCCATCGAGAATTCATCGCTGAAATGGAAACCCTGGGTAAGGTAAACCACCAGAATGTCGTTCCATTACTCGGGTACTGCTCTCTTGGCGAGGAGAAGCTCCTTGTTTACGAGTATATGGTAAATGGGAGCCTGGACATTTGGCTGAGAAATCGGACTGGGGATCTTGAAGTCCTAGATTGGGACAGACGTTTCAAAATCGCAATGGGTGCAGCCCGCGGCCTTGCTTTTCTTCACCATGGATTCAGCCCCCACATCATCCACAGGGACATCAAAGCCAGCAACATCTTGCTCAACGAAGACTTCGAGCCCAAAGTTGCAGACTTTGGACTCGCAAGGTTGATCAGTGCCTGTGAGACTCACATTAGTACTGATATAGCCGGAACATTTGGCTACATTCCACCGGAGTATGGACAGAGCGGGCGGTCTACCACAAAAGGAGACGTCTATAGTTTTGGCGTGATACTGCTCGAACTGGTGACAGGGAAAGAGCCAACCGGACCCGATTTCAAAGACTTGGAAGGCGGGAATCTAGTCGGGTGGGTTTTTCAGATGATGAAGAAGGCGAAGGCAGCTGATGTTCTTGATCCGGTAGTACTCGACGCGGATTCGAAGCGCAGGATGGTTCAAGTGTTGAAGATTGCCTGTGTGTGCCTCTCTGATAACCCAGCTCAGAGGCCTACGATGCTTCAAGTGTTGAAGTTTCTCAAAGAGATTAGTGATGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001708 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005575 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009556 GO:0009653 GO:0009791 GO:0009908 GO:0009987 GO:0010234 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019953 GO:0022402 GO:0022414 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0034293 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0043934 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044703 GO:0045165 GO:0046777 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048656 GO:0048657 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051704 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140096 GO:1901564 GO:1903046 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1301

Amino Acids

141.35

Weight (kDa)

5.54

Isoelectric Point (pI)

31.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	32 - 68	2.6e-10	Leucine rich repeat N-terminal domain
LRR_14	PF23598	158 - 249	2.1e-09	Leucine-rich repeat region
LRR_14	PF23598	247 - 356	1.7e-11	Leucine-rich repeat region
LRR_8	PF13855	702 - 742	9.6e-07	Leucine rich repeat
Pkinase	PF00069	1025 - 1292	4.4e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	1026 - 1293	6.3e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012974)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07280
fragaria_vesca	FvH4_7g29180
malus_domestica	MD01G1198500.v1.1 MD07G1266400.v1.1
prunus_persica	Prupe.2G292000_v2.0.a1
pyrus_communis	pycom01g20870 pycom07g24420
rosa_chinensis	RchiOBHm_Chr1g0376951
rosa_laevigata	RLG00000026557
rosa_multiflora	Rmu_sc0026264.1_g000001 Rmu_ssc0000155.1_g000035
rosa_roxburghii	Rroxscaffold_4G00281610
rosa_rugosa	Rorug01G0399800
rosa_samantha	Rh1AG417000 Rh1BG376400 Rh1CG390200 Rh1DG407100
rosa_wichuraiana	Rw1G036600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	3621
Acc16I	TGCGCA	1 cut(s)	1785
Acc36I	ACCTGC	2 cut(s)	1761, 2910
AccB1I	GGYRCC	1 cut(s)	2231
AccB7I	CCANNNNNTGG	3 cut(s)	488, 2105, 2409
AccBSI	CCGCTC	1 cut(s)	3594
AccI	GTMKAC	1 cut(s)	3602
AccII	CGCG	5 cut(s)	774, 793, 3143, 3393, 3779
AccIII	TCCGGA	1 cut(s)	1270
AclWI	GGATC	8 cut(s)	729, 1762, 2423, 2508, 2882, 2891, 3345, 3755
AcoI	YGGCCR	2 cut(s)	211, 1711
AcsI	RAATTY	9 cut(s)	493, 801, 1498, 1507, 2019, 2512, 2613, 2752, 3185
AcuI	CTGAAG	3 cut(s)	464, 737, 2931
AcyI	GRCGYC	2 cut(s)	5, 3618
AfaI	GTAC	8 cut(s)	809, 890, 1812, 1862, 3110, 3251, 3544, 3771
AflIII	ACRYGT	3 cut(s)	1251, 1347, 1903
AgeI	ACCGGT	2 cut(s)	1721, 2213
AjiI	CACGTC	1 cut(s)	338
AjnI	CCWGG	8 cut(s)	585, 591, 1130, 1383, 2541, 2801, 3207, 3306
AjuI	GAANNNNNNNTTGG	2 cut(s)	518, 550
AloI	GAACNNNNNNTCC	4 cut(s)	1097, 1129, 2375, 2407
Alw21I	GWGCWC	8 cut(s)	522, 735, 972, 1068, 1721, 1743, 2162, 2836
Alw26I	GTCTC	4 cut(s)	431, 3081, 3524, 3609
Alw44I	GTGCAC	1 cut(s)	1717
AlwI	GGATC	8 cut(s)	729, 1762, 2423, 2508, 2882, 2891, 3345, 3755
AlwNI	CAGNNNCTG	4 cut(s)	586, 940, 3019, 3525
Ama87I	CYCGRG	4 cut(s)	610, 1330, 2561, 3245
Aor13HI	TCCGGA	1 cut(s)	1270
ApaLI	GTGCAC	1 cut(s)	1717
ApoI	RAATTY	9 cut(s)	493, 801, 1498, 1507, 2019, 2512, 2613, 2752, 3185
ArsI	GACNNNNNNTTYG	4 cut(s)	1334, 1366, 3080, 3112
AsiGI	ACCGGT	2 cut(s)	1721, 2213
Asp700I	GAANNNNTTC	2 cut(s)	111, 1161
AspLEI	GCGC	4 cut(s)	692, 1409, 1786, 3792
AsuC2I	CCSGG	5 cut(s)	574, 1189, 1772, 1883, 2169
AsuII	TTCGAA	4 cut(s)	1529, 1676, 1847, 3785
AvaI	CYCGRG	4 cut(s)	610, 1330, 2561, 3245
AvaII	GGWCC	4 cut(s)	589, 1547, 2028, 3674
AxyI	CCTNAGG	1 cut(s)	1059
BaeGI	GKGCMC	2 cut(s)	1478, 1721
BanI	GGYRCC	1 cut(s)	2231
BanII	GRGCYC	6 cut(s)	522, 735, 972, 1068, 1743, 3486
BauI	CACGAG	1 cut(s)	157
BbsI	GAAGAC	2 cut(s)	952, 3480
Bbv12I	GWGCWC	8 cut(s)	522, 735, 972, 1068, 1721, 1743, 2162, 2836
BbvCI	CCTCAGC	1 cut(s)	1226
BccI	CCATC	7 cut(s)	1507, 2294, 2444, 2865, 3122, 3186, 3790
BceAI	ACGGC	2 cut(s)	820, 1562
BcgI	CGANNNNNNTGC	2 cut(s)	2980, 3014
BciT130I	CCWGG	8 cut(s)	587, 593, 1132, 1385, 2543, 2803, 3209, 3308
BclI	TGATCA	2 cut(s)	1992, 3516
BcnI	CCSGG	5 cut(s)	574, 1189, 1772, 1883, 2169
BcoDI	GTCTC	4 cut(s)	431, 3081, 3524, 3609
BfaI	CTAG	5 cut(s)	299, 434, 2837, 3350, 3707
BfmI	CTRYAG	4 cut(s)	578, 1629, 2862, 3622
BfuAI	ACCTGC	2 cut(s)	1761, 2910
BglI	GCCNNNNNGGC	2 cut(s)	210, 1132
BlpI	GCTNAGC	4 cut(s)	990, 1757, 1790, 3155
BmcAI	AGTACT	2 cut(s)	3544, 3771
Bme18I	GGWCC	4 cut(s)	589, 1547, 2028, 3674
BmeT110I	CYCGRG	4 cut(s)	610, 1330, 2561, 3245
BmgBI	CACGTC	1 cut(s)	338
BmiI	GGNNCC	9 cut(s)	454, 536, 1263, 2030, 2233, 2699, 2982, 3305, 3676
BmrI	ACTGGG	2 cut(s)	196, 3342
BmsI	GCATC	5 cut(s)	753, 1014, 2544, 3000, 3849
BmuI	ACTGGG	2 cut(s)	196, 3342
BpiI	GAAGAC	2 cut(s)	952, 3480
BplI	GAGNNNNNCTC	2 cut(s)	1052, 1084
BpmI	CTGGAG	2 cut(s)	2082, 2520
Bpu10I	CCTNAGC	4 cut(s)	362, 1226, 1704, 2176
Bpu1102I	GCTNAGC	4 cut(s)	990, 1757, 1790, 3155
Bpu14I	TTCGAA	4 cut(s)	1529, 1676, 1847, 3785
BpuEI	CTTGAG	3 cut(s)	1261, 2452, 3008
BpuMI	CCSGG	5 cut(s)	574, 1189, 1772, 1883, 2169
Bsa29I	ATCGAT	1 cut(s)	1160
BsaBI	GATNNNNATC	1 cut(s)	2499
BsaHI	GRCGYC	2 cut(s)	5, 3618
Bse118I	RCCGGY	7 cut(s)	1403, 1713, 1721, 2213, 2364, 2720, 2889
Bse1I	ACTGG	3 cut(s)	191, 3337, 3654
Bse21I	CCTNAGG	1 cut(s)	1059
Bse3DI	GCAATG	3 cut(s)	1291, 1808, 3384
Bse8I	GATNNNNATC	1 cut(s)	2499
BseAI	TCCGGA	1 cut(s)	1270
BseBI	CCWGG	8 cut(s)	587, 593, 1132, 1385, 2543, 2803, 3209, 3308
BseCI	ATCGAT	1 cut(s)	1160
BseGI	GGATG	5 cut(s)	1878, 1990, 3133, 3432, 3801
BseJI	GATNNNNATC	1 cut(s)	2499
BseMI	GCAATG	3 cut(s)	1291, 1808, 3384
BseNI	ACTGG	3 cut(s)	191, 3337, 3654
BseRI	GAGGAG	5 cut(s)	145, 298, 1076, 2538, 3281
BseSI	GKGCMC	2 cut(s)	1478, 1721
BseYI	CCCAGC	2 cut(s)	994, 3841
BsgI	GTGCAG	1 cut(s)	3405
Bsh1236I	CGCG	5 cut(s)	774, 793, 3143, 3393, 3779
BshNI	GGYRCC	1 cut(s)	2231
BshTI	ACCGGT	2 cut(s)	1721, 2213
BshVI	ATCGAT	1 cut(s)	1160
BsiHKAI	GWGCWC	8 cut(s)	522, 735, 972, 1068, 1721, 1743, 2162, 2836
BsiHKCI	CYCGRG	4 cut(s)	610, 1330, 2561, 3245
BslFI	GGGAC	4 cut(s)	1063, 2014, 3371, 3455
BsmAI	GTCTC	4 cut(s)	431, 3081, 3524, 3609
BsmBI	CGTCTC	2 cut(s)	3081, 3609
BsmFI	GGGAC	4 cut(s)	1063, 2014, 3371, 3455
BsmI	GAATGC	1 cut(s)	2803
BsoBI	CYCGRG	4 cut(s)	610, 1330, 2561, 3245
Bsp119I	TTCGAA	4 cut(s)	1529, 1676, 1847, 3785
Bsp13I	TCCGGA	1 cut(s)	1270
Bsp1407I	TGTACA	1 cut(s)	3108
Bsp1720I	GCTNAGC	4 cut(s)	990, 1757, 1790, 3155
Bsp19I	CCATGG	4 cut(s)	538, 660, 2403, 3412
BspDI	ATCGAT	1 cut(s)	1160
BspEI	TCCGGA	1 cut(s)	1270
BspFNI	CGCG	5 cut(s)	774, 793, 3143, 3393, 3779
BspLI	GGNNCC	9 cut(s)	454, 536, 1263, 2030, 2233, 2699, 2982, 3305, 3676
BspMAI	CTGCAG	1 cut(s)	582
BspMI	ACCTGC	2 cut(s)	1761, 2910
BspPI	GGATC	8 cut(s)	729, 1762, 2423, 2508, 2882, 2891, 3345, 3755
BspQI	GCTCTTC	1 cut(s)	1269
BspT104I	TTCGAA	4 cut(s)	1529, 1676, 1847, 3785
BspT107I	GGYRCC	1 cut(s)	2231
BsrBI	CCGCTC	1 cut(s)	3594
BsrDI	GCAATG	3 cut(s)	1291, 1808, 3384
BsrFI	RCCGGY	7 cut(s)	1403, 1713, 1721, 2213, 2364, 2720, 2889
BsrGI	TGTACA	1 cut(s)	3108
BsrI	ACTGG	3 cut(s)	191, 3337, 3654
BssAI	RCCGGY	7 cut(s)	1403, 1713, 1721, 2213, 2364, 2720, 2889
BssNI	GRCGYC	2 cut(s)	5, 3618
BssSI	CACGAG	1 cut(s)	157
BssT1I	CCWWGG	6 cut(s)	513, 538, 660, 2024, 2403, 3412
Bst2BI	CACGAG	1 cut(s)	157
Bst2UI	CCWGG	8 cut(s)	587, 593, 1132, 1385, 2543, 2803, 3209, 3308
Bst4CI	ACNGT	5 cut(s)	148, 1004, 1970, 3106, 3139
Bst6I	CTCTTC	5 cut(s)	278, 624, 840, 1269, 1629
BstACI	GRCGYC	2 cut(s)	5, 3618
BstAPI	GCANNNNNTGC	3 cut(s)	2858, 3019, 3383
BstAUI	TGTACA	1 cut(s)	3108
BstBI	TTCGAA	4 cut(s)	1529, 1676, 1847, 3785
BstC8I	GCNNGC	9 cut(s)	1405, 1782, 2306, 2330, 2718, 2891, 3391, 3454, 3596
BstDSI	CCRYGG	5 cut(s)	538, 660, 2403, 3391, 3412
BstENI	CCTNNNNNAGG	1 cut(s)	3212
BstF5I	GGATG	5 cut(s)	1878, 1990, 3133, 3432, 3801
BstFNI	CGCG	5 cut(s)	774, 793, 3143, 3393, 3779
BstHHI	GCGC	4 cut(s)	692, 1409, 1786, 3792
BstMAI	GTCTC	4 cut(s)	431, 3081, 3524, 3609
BstNI	CCWGG	8 cut(s)	587, 593, 1132, 1385, 2543, 2803, 3209, 3308
BstNSI	RCATGY	4 cut(s)	1255, 1907, 2080, 2332
BstSFI	CTRYAG	4 cut(s)	578, 1629, 2862, 3622
BstSLI	GKGCMC	2 cut(s)	1478, 1721
BstUI	CGCG	5 cut(s)	774, 793, 3143, 3393, 3779
BstV2I	GAAGAC	2 cut(s)	952, 3480
BstX2I	RGATCY	3 cut(s)	721, 2500, 3337
BstXI	CCANNNNNNTGG	1 cut(s)	2471
BstYI	RGATCY	3 cut(s)	721, 2500, 3337
Bsu15I	ATCGAT	1 cut(s)	1160
Bsu36I	CCTNAGG	1 cut(s)	1059
BsuTUI	ATCGAT	1 cut(s)	1160
BtgI	CCRYGG	5 cut(s)	538, 660, 2403, 3391, 3412
BtgZI	GCGATG	2 cut(s)	2301, 3175
BtrI	CACGTC	1 cut(s)	338
BtsCI	GGATG	5 cut(s)	1878, 1990, 3133, 3432, 3801
BtsI	GCAGTG	2 cut(s)	178, 500
BtsIMutI	CAGTG	5 cut(s)	178, 500, 963, 2089, 3526
BveI	ACCTGC	2 cut(s)	1761, 2910
Cac8I	GCNNGC	9 cut(s)	1405, 1782, 2306, 2330, 2718, 2891, 3391, 3454, 3596
CaiI	CAGNNNCTG	4 cut(s)	586, 940, 3019, 3525
CfoI	GCGC	4 cut(s)	692, 1409, 1786, 3792
Cfr10I	RCCGGY	7 cut(s)	1403, 1713, 1721, 2213, 2364, 2720, 2889
Cfr42I	CCGCGG	1 cut(s)	3394
ClaI	ATCGAT	1 cut(s)	1160
CseI	GACGC	2 cut(s)	1381, 3785
CsiI	ACCWGGT	1 cut(s)	1383
Csp6I	GTAC	8 cut(s)	808, 889, 1811, 1861, 3109, 3250, 3543, 3770
CspAI	ACCGGT	2 cut(s)	1721, 2213
CviQI	GTAC	8 cut(s)	808, 889, 1811, 1861, 3109, 3250, 3543, 3770
EaeI	YGGCCR	2 cut(s)	211, 1711
Eam1104I	CTCTTC	5 cut(s)	278, 624, 840, 1269, 1629
EarI	CTCTTC	5 cut(s)	278, 624, 840, 1269, 1629
EciI	GGCGGA	3 cut(s)	160, 1689, 1790
Ecl136II	GAGCTC	5 cut(s)	520, 733, 970, 1066, 1741
Eco130I	CCWWGG	6 cut(s)	513, 538, 660, 2024, 2403, 3412
Eco147I	AGGCCT	1 cut(s)	3853
Eco24I	GRGCYC	6 cut(s)	522, 735, 972, 1068, 1743, 3486
Eco32I	GATATC	1 cut(s)	1554
Eco47I	GGWCC	4 cut(s)	589, 1547, 2028, 3674
Eco53kI	GAGCTC	5 cut(s)	520, 733, 970, 1066, 1741
Eco57I	CTGAAG	3 cut(s)	464, 737, 2931
Eco81I	CCTNAGG	1 cut(s)	1059
Eco88I	CYCGRG	4 cut(s)	610, 1330, 2561, 3245
EcoICRI	GAGCTC	5 cut(s)	520, 733, 970, 1066, 1741
EcoNI	CCTNNNNNAGG	1 cut(s)	3212
EcoO109I	RGGNCCY	2 cut(s)	254, 2028
EcoRI	GAATTC	2 cut(s)	1498, 3185
EcoRII	CCWGG	8 cut(s)	585, 591, 1130, 1383, 2541, 2801, 3207, 3306
EcoRV	GATATC	1 cut(s)	1554
EcoT14I	CCWWGG	6 cut(s)	513, 538, 660, 2024, 2403, 3412
EcoT38I	GRGCYC	6 cut(s)	522, 735, 972, 1068, 1743, 3486
ErhI	CCWWGG	6 cut(s)	513, 538, 660, 2024, 2403, 3412
Esp3I	CGTCTC	2 cut(s)	3081, 3609
FalI	AAGNNNNNCTT	6 cut(s)	99, 131, 2414, 2446, 2630, 2662
FaqI	GGGAC	4 cut(s)	1063, 2014, 3371, 3455
FauI	CCCGC	7 cut(s)	486, 779, 1282, 1754, 3398, 3587, 3692
FbaI	TGATCA	2 cut(s)	1992, 3516
FblI	GTMKAC	1 cut(s)	3602
FokI	GGATG	5 cut(s)	1865, 1997, 3140, 3419, 3808
FriOI	GRGCYC	6 cut(s)	522, 735, 972, 1068, 1743, 3486
FspBI	CTAG	5 cut(s)	299, 434, 2837, 3350, 3707
FspI	TGCGCA	1 cut(s)	1785
GlaI	GCGC	4 cut(s)	691, 1408, 1785, 3791
GsaI	CCCAGC	2 cut(s)	998, 3845
GsuI	CTGGAG	2 cut(s)	2082, 2520
HgaI	GACGC	2 cut(s)	1381, 3785
HhaI	GCGC	4 cut(s)	692, 1409, 1786, 3792
Hin1I	GRCGYC	2 cut(s)	5, 3618
Hin6I	GCGC	4 cut(s)	690, 1407, 1784, 3790
HinP1I	GCGC	4 cut(s)	690, 1407, 1784, 3790
HincII	GTYRAC	4 cut(s)	426, 870, 1257, 3040
HindII	GTYRAC	4 cut(s)	426, 870, 1257, 3040
HindIII	AAGCTT	5 cut(s)	2120, 2173, 2191, 2201, 3026
HpaI	GTTAAC	1 cut(s)	426
Hpy99I	CGWCG	3 cut(s)	10, 3002, 3779
HpyCH4III	ACNGT	5 cut(s)	148, 1004, 1970, 3106, 3139
HpyCH4IV	ACGT	9 cut(s)	337, 666, 738, 1052, 1088, 1349, 2997, 3364, 3618
HpySE526I	ACGT	9 cut(s)	337, 666, 738, 1052, 1088, 1349, 2997, 3364, 3618
Hsp92I	GRCGYC	2 cut(s)	5, 3618
HspAI	GCGC	4 cut(s)	690, 1407, 1784, 3790
Kpn2I	TCCGGA	1 cut(s)	1270
KroI	GCCGGC	2 cut(s)	1403, 2889
KroNI	GCCGGC	2 cut(s)	1405, 2891
Ksp22I	TGATCA	2 cut(s)	1992, 3516
KspAI	GTTAAC	1 cut(s)	426
KspI	CCGCGG	1 cut(s)	3394
LguI	GCTCTTC	1 cut(s)	1269
LmnI	GCTCC	9 cut(s)	517, 1063, 1429, 1738, 1777, 2455, 2980, 3279, 3303
LweI	GCATC	5 cut(s)	753, 1014, 2544, 3000, 3849
MabI	ACCWGGT	1 cut(s)	1383
MaeI	CTAG	5 cut(s)	299, 434, 2837, 3350, 3707
MaeII	ACGT	9 cut(s)	337, 666, 738, 1052, 1088, 1349, 2997, 3364, 3618
MaeIII	GTNAC	6 cut(s)	217, 765, 1230, 1601, 2723, 3652
MbiI	CCGCTC	1 cut(s)	3594
MfeI	CAATTG	2 cut(s)	1338, 1796
MflI	RGATCY	3 cut(s)	721, 2500, 3337
MlyI	GAGTC	4 cut(s)	225, 433, 3498, 3526
MmeI	TCCRAC	7 cut(s)	336, 549, 1485, 1888, 2643, 2806, 3105
MroI	TCCGGA	1 cut(s)	1270
MroNI	GCCGGC	2 cut(s)	1403, 2889
MroXI	GAANNNNTTC	2 cut(s)	111, 1161
MseI	TTAA	7 cut(s)	425, 2042, 2205, 2282, 2370, 2637, 3147
MslI	CAYNNNNRTG	3 cut(s)	84, 2240, 3228
MspA1I	CMGCKG	7 cut(s)	1540, 1612, 1761, 1789, 3019, 3393, 3749
MunI	CAATTG	2 cut(s)	1338, 1796
Mva1269I	GAATGC	1 cut(s)	2803
MvaI	CCWGG	8 cut(s)	587, 593, 1132, 1385, 2543, 2803, 3209, 3308
MvnI	CGCG	5 cut(s)	774, 793, 3143, 3393, 3779
NaeI	GCCGGC	2 cut(s)	1405, 2891
NciI	CCSGG	5 cut(s)	574, 1189, 1772, 1883, 2169
NcoI	CCATGG	4 cut(s)	538, 660, 2403, 3412
NgoMIV	GCCGGC	2 cut(s)	1403, 2889
NlaIV	GGNNCC	9 cut(s)	454, 536, 1263, 2030, 2233, 2699, 2982, 3305, 3676
NmeAIII	GCCGAG	2 cut(s)	239, 445
NmuCI	GTSAC	2 cut(s)	217, 3652
NsbI	TGCGCA	1 cut(s)	1785
NspI	RCATGY	4 cut(s)	1255, 1907, 2080, 2332
NspV	TTCGAA	4 cut(s)	1529, 1676, 1847, 3785
PaeI	GCATGC	1 cut(s)	2332
PaeR7I	CTCGAG	1 cut(s)	2561
PasI	CCCWGGG	1 cut(s)	3208
PceI	AGGCCT	1 cut(s)	3853
PciI	ACATGT	2 cut(s)	1251, 1903
PciSI	GCTCTTC	1 cut(s)	1269
PcsI	WCGNNNNNNNCGW	3 cut(s)	893, 1853, 3477
PctI	GAATGC	1 cut(s)	2803
PdiI	GCCGGC	2 cut(s)	1405, 2891
PdmI	GAANNNNTTC	2 cut(s)	111, 1161
PflFI	GACNNNGTC	1 cut(s)	188
PflMI	CCANNNNNTGG	3 cut(s)	488, 2105, 2409
PfoI	TCCNGGA	3 cut(s)	585, 1187, 1881
PinAI	ACCGGT	2 cut(s)	1721, 2213
PleI	GAGTC	4 cut(s)	224, 433, 3498, 3526
PpsI	GAGTC	4 cut(s)	224, 433, 3498, 3526
PpuMI	RGGWCCY	1 cut(s)	2028
PscI	ACATGT	2 cut(s)	1251, 1903
Psp124BI	GAGCTC	5 cut(s)	522, 735, 972, 1068, 1743
Psp5II	RGGWCCY	1 cut(s)	2028
Psp6I	CCWGG	8 cut(s)	585, 591, 1130, 1383, 2541, 2801, 3207, 3306
PspFI	CCCAGC	2 cut(s)	994, 3841
PspGI	CCWGG	8 cut(s)	585, 591, 1130, 1383, 2541, 2801, 3207, 3306
PspN4I	GGNNCC	9 cut(s)	454, 536, 1263, 2030, 2233, 2699, 2982, 3305, 3676
PspPPI	RGGWCCY	1 cut(s)	2028
PspXI	VCTCGAGB	1 cut(s)	2561
PstI	CTGCAG	1 cut(s)	582
PstNI	CAGNNNCTG	4 cut(s)	586, 940, 3019, 3525
PsuI	RGATCY	3 cut(s)	721, 2500, 3337
PsyI	GACNNNGTC	1 cut(s)	188
PvuII	CAGCTG	4 cut(s)	1540, 1612, 1789, 3749
RsaI	GTAC	8 cut(s)	809, 890, 1812, 1862, 3110, 3251, 3544, 3771
RsaNI	GTAC	8 cut(s)	808, 889, 1811, 1861, 3109, 3250, 3543, 3770
RseI	CAYNNNNRTG	3 cut(s)	84, 2240, 3228
SacI	GAGCTC	5 cut(s)	522, 735, 972, 1068, 1743
SacII	CCGCGG	1 cut(s)	3394
SapI	GCTCTTC	1 cut(s)	1269
SaqAI	TTAA	7 cut(s)	425, 2042, 2205, 2282, 2370, 2637, 3147
ScaI	AGTACT	2 cut(s)	3544, 3771
SchI	GAGTC	4 cut(s)	225, 433, 3498, 3526
SexAI	ACCWGGT	1 cut(s)	1383
SfaNI	GCATC	5 cut(s)	753, 1014, 2544, 3000, 3849
SfcI	CTRYAG	4 cut(s)	578, 1629, 2862, 3622
Sfr274I	CTCGAG	1 cut(s)	2561
Sfr303I	CCGCGG	1 cut(s)	3394
SfuI	TTCGAA	4 cut(s)	1529, 1676, 1847, 3785
SgrBI	CCGCGG	1 cut(s)	3394
SinI	GGWCC	4 cut(s)	589, 1547, 2028, 3674
SlaI	CTCGAG	1 cut(s)	2561
SmiMI	CAYNNNNRTG	3 cut(s)	84, 2240, 3228
SmlI	CTYRAG	4 cut(s)	1240, 2431, 2561, 3023
SmoI	CTYRAG	4 cut(s)	1240, 2431, 2561, 3023
SphI	GCATGC	1 cut(s)	2332
SseBI	AGGCCT	1 cut(s)	3853
SspI	AATATT	2 cut(s)	877, 1668
SspMI	CTAG	5 cut(s)	299, 434, 2837, 3350, 3707
SstI	GAGCTC	5 cut(s)	522, 735, 972, 1068, 1743
StuI	AGGCCT	1 cut(s)	3853
StyI	CCWWGG	6 cut(s)	513, 538, 660, 2024, 2403, 3412
TaaI	ACNGT	5 cut(s)	148, 1004, 1970, 3106, 3139
TaiI	ACGT	9 cut(s)	340, 669, 741, 1055, 1091, 1352, 3000, 3367, 3621
TatI	WGTACW	4 cut(s)	1860, 3108, 3542, 3769
TauI	GCSGC	5 cut(s)	166, 327, 796, 3019, 3396
Tru1I	TTAA	7 cut(s)	425, 2042, 2205, 2282, 2370, 2637, 3147
Tru9I	TTAA	7 cut(s)	425, 2042, 2205, 2282, 2370, 2637, 3147
TscAI	CASTG	5 cut(s)	185, 507, 970, 2089, 3526
TseFI	GTSAC	2 cut(s)	217, 3652
Tsp45I	GTSAC	2 cut(s)	217, 3652
TspDTI	ATGAA	7 cut(s)	669, 2304, 2441, 2495, 2923, 3178, 3746
TspGWI	ACGGA	3 cut(s)	225, 2507, 3105
TspRI	CASTG	5 cut(s)	185, 507, 970, 2089, 3526
Tth111I	GACNNNGTC	1 cut(s)	188
Van91I	CCANNNNNTGG	3 cut(s)	488, 2105, 2409
VneI	GTGCAC	1 cut(s)	1717
VpaK11BI	GGWCC	4 cut(s)	589, 1547, 2028, 3674
XagI	CCTNNNNNAGG	1 cut(s)	3212
XapI	RAATTY	9 cut(s)	493, 801, 1498, 1507, 2019, 2512, 2613, 2752, 3185
XceI	RCATGY	4 cut(s)	1255, 1907, 2080, 2332
XhoI	CTCGAG	1 cut(s)	2561
XmiI	GTMKAC	1 cut(s)	3602
XmnI	GAANNNNTTC	2 cut(s)	111, 1161
XspI	CTAG	5 cut(s)	299, 434, 2837, 3350, 3707
ZraI	GACGTC	1 cut(s)	3619
ZrmI	AGTACT	2 cut(s)	3544, 3771

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.