Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0012974

Physical Location & Seq

Genomic Coordinates Forward (+)

33481811 .. 33485724

Gene Structure 3914 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1266400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3894 bp

ATGGCGTCGATAAAGCCTCTGTTTTTAGTTATTTTCATAGTTTTTGAGTCCCTCATTCTACTCGCCAATGGCGTTTCCCGCCAAAACGTCCAAAACCCTGAACGGAAAGCTCTCCTTTCCTTCAAAACCGTCCTCGGAAACCCCGCAGTTCTCTCCTCGTGGCGGCCCTCCGCCTCGCACTGCGACTGGGTCGGCGTTTCCTGCCAATTGGGCCGAGTCACCGTGCTCGCCCTCCCGTCTTTCTCCCTCAGAGGCCCGCTCCCTCCCTCCCTCTTCTCCCTCTCCAACCTCGCCGTGCTGGACCTCTCCTCCAACCTACTTCACGGTAAAATTCCACTTCAAATTCTCAACCTCAAACACCTAAACCACCTCTGCCTCACTGGTAACCAGCTCTCCGGCGAAATACCGACCCAAATCGCGGAGTTGACTGAGCTGGAGACCCTGAAGCTCGGAACCAACTTTTTCACCGGGAAAATCCCGCCGGAGCTGGGATATTTATCTCTACTTCAAACCCTGGAGCTCTCCAGTAATGACTTCTATGGAGAAATTCCGACCCAGATTGGAAATTTGACCCGGCTGAAGTTCCTGGACCTGGGCAACAACGTCCTCTCAGGTTCTCTTCCCTCTGACCTGTTTCCAAAGCTTCAATCTATAGCTTCCATGGACGTATCACGCAATTCACTCTCTGGGGAAATTCCACCGTCAATTGGTAGCCTGAAGAATCTCACGGACCTCTACATTGGCAACAATCATATCTCCGGCCCGTTGCCCCGCGAAATCGGAGAGCTTTCGCGGCTCGAAATTTTGGACGCTGCTTCCTGTTCCATAACAGGGCCGTTGCCGGAAGAGATTTCTAAGCTGGAATCGTTGACCAAAATTGACCTCTCGTACAACCCATTGAGGTGTTCGTTCCCAAAAGGCGTAGGGAAGTTGCAGAACATGAGCATTTTCGCTTTGGTTTTCACAGAGCTTAATGGGTCTGTTCCTGCTGAGCTAGGGAACTGTAAAAGTTTGACGACTTTGATGCTTTCGTTCAATTCGCTTTCGGGGCCGTTGCCTGAAGAGCTCTCTGAGCTTCCACTGATGACTTTTTCCACTGAAAAGAACCAGCTTTCGGGCATCTTGCCTTCCTGGCTCGGAAAATTGAAGCAAGTCGAATCGCTTCTGCTTTCGAGCAATCTGTTTTCCGGGAAAATTCCACCGGAGATTGGCAACTGCTCTATGCTGAGGTTACTTAGCTTGAGCAATAACTTGTTGACGGGTCCGATTCCGGAAGAGATTTGCAATGCGGGTTCGCTTTTGGAGATTGATCTCGATAGCAATTACCTCTCGGGAACAATTGACAGCACGTTTGTCAAGTGCAAGAACTTGTCGCAGCTCAACTTGGTGAACAATCGGATTGCCGGCGCAATCCCTCATTACCTTTCGGAGCTTCCACTCATGGTGATCGACCTTGATTCGAACAACTTCACGGGCACAATACCGAAAAGTCTTTGGAATTCGAAGAATTTGATGGAGTTCACAGCTTCGAACAATCAGCTGAGCGGTTCAATTTCGAATAAGATTCGTCGGGCAGCTGTCTTGGAAAGGCTCGTCCTCAGTAACAATCGCCTGACAGGAAAACTTCCTAAAGAGATCGGAAATGTCAGCAGCCTTTCTGTTCTAATATTGAATTCGAATCTTCTGGAAGGGAACATTCCGGCGGAGCTTAGCAAGTGCACCGGTCTTACTACATTGGATCTGGGAAACAATCAGCTTAGCGGCTCTATCCCAACGGAGCTGGCAGAGCTAAGCCAATTGCAATGTCTGGTACTTTCTCACAACAATCTATCTGGTTCAATTCCTTTGAAAAAATCGTTGTATTTTCGACAAGCAAATATCCCGGTTTTGAGCTTTGTGCAACATGTTGGAGTGTTTGATCTGTCCTACAATAGGTTGTCGGGTGCAATACCTGAAGATTTGGGTCACTGTGTTATTCTGGTGGATGTTTTGATCAGCAACAACCTGCTCTCTGGTGAAATTCCAAGGTCCCTCGCTCGTTTAACAAACCTTACAACTTTGGATTTGTCTGGGAACATGCTCAGTGGTTCCATTCCTCCTGAGTTTGGTGATTCGCCGAAGCTTCAAGGCTTGAATCTGGGAAATAACCAGCTTACGAGTACCATCCCTGGAAGCTTTGGTCGTTTAGGAAGCTTGGTGAAGCTTAATTTGACTGGTAATAAGTTATCTGGTTCTGTACCTGTGAGCTTTGGGGAATTGAAAGAGCTAACTCACTTGGATTTAAGCTCAAACAAGCTAGATGGCGAGCTTCCTTCATCGCTGTCAAGCATGCAGAATCTCGTGGGGCTGTATGTTCAGGAGAACCGCCTTAATGGTGGAGTTGATAAGCTGTTCTCGAATACCATAGCTTGGAGGATCGAAAAGATGAACTTGAGCAATAATTTCTTTGATGGGGTGCTGCCCCATTCTCTTGGTAATCTGTCATACTTGACGGATTTGGATCTTCATTCAAATTCGTTCCAAGGAGAGATTCCTCCAGACCTTGGGAATTTGGTGCAACTTGAGTATTTTGATGTTTCAAGTAACAAGCTCTCTGGACAGATTCCAGAGAAAATTTGCAGCCTCATCAATCTGTTTTACATGAACTTGGCGGAAAATAGGTTGGAAGGGCCCCTTCCTAATAGTGGTATTTGCCGGAACCTGTCAGAAAAATCGCTTGCCGGTAACAAGAAACTGTGTGGGAGAAGTAAAGATTTGGATTGCCATGCCAAGAGTTTTGATGAATTAGCATTGTTTAATGCCCAGGGAGTCGCTGCTATTGTAGTTGGAAGTGCTCTAATCTTTATTGTTGCAGCTTATGCTGTAGTAAGATGGATCACCCGAAGCAGCCGGCATGATCCTGAAGAAGATGAAGAAACCAGGTTGACTAGTTTCATGGAACATTATTTTCTCAGTAGCAGCAGATCAAAGGAGCTTCTGAGCATCAACGTTGCCATGTCTCAGCAGCCGCTTCTCAAGCTAACTTTAGTTGACATTCTTGAAGCCACCAACAACTTCTGCAAGACAAACATAATAGGAGATGGAGGCTTTGGAACCGTGTACAAGGCCACGTTGTCAGATGGGAAAGCCGTTGCGGTTAAGAAGCTAAATGAGTATAAAACTCAGGGACATCGAGAATTCATGGCTGAAATGGAAACCCTGGGGAAGGTAAACCACCAAAATCTCGTTCCATTACTCGGGTACTGTTCTCTTGGCGAGGAGAAGCTCCTTGTTTATGAGTATATGGTAAATGGGAGCCTCGACATTTGGCTGAGAAATCGGAGTGGAGATCCTGAAGGCCTAGACTGGGACAGACGTTTCAAAATTGCAACGGGTGCAGCCCGCGGCCTGGCTTTTCTTCACCATGGATTCGGCCCCCATATCATCCACAGGGATATCAAAGCCAGCAACATCTTGCTCAACGAAGACTTTGAACCCAAAGTTGCAGACTTTGGACTTGCAAGATTGATCAATGCCTGTGAGACTCACATTAGCACCGATATAGCCGGAACATTTGGCTACATTCCACCTGAGTATGGACAGAGCGGGCGGTCTACCACAAAGGGAGATGTCTATAGCTTTGGTGTGATACTGCTCGAGTTGGTGACAGGGAAAGAGCCAACCGGACCCGATTTCAAAGATTTGGAAGGCGGGAATTTAGTGGGGTGGGTTTTTCAGATGCTGAAGAAGGGGAAGGCTGCTGATGTTCTTGATCCGATGGTGCTTGACGCGGATTCGAAGCGCACGATGGTTCAAGTGTTGAAGATTGCCTGTGTGTGCGTCTCTGATAACCCAGCTCAGAGGCCTACAATGCTTCAAGTGCTCAAGTTTCTCAAAGGGATTAATGACGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001708 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005575 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009556 GO:0009653 GO:0009791 GO:0009908 GO:0009987 GO:0010234 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019953 GO:0022402 GO:0022414 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0034293 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0043934 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044703 GO:0045165 GO:0046777 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048656 GO:0048657 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051704 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140096 GO:1901564 GO:1903046 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1298

Amino Acids

141.12

Weight (kDa)

5.77

Isoelectric Point (pI)

34.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	32 - 68	5.3e-10	Leucine rich repeat N-terminal domain
LRR_14	PF23598	157 - 247	8.3e-08	Leucine-rich repeat region
LRR_14	PF23598	186 - 273	1e-08	Leucine-rich repeat region
LRR_14	PF23598	236 - 388	3.5e-10	Leucine-rich repeat region
LRR_8	PF13855	551 - 610	9e-06	Leucine rich repeat
LRR_8	PF13855	702 - 742	1.9e-07	Leucine rich repeat
LRR_8	PF13855	806 - 864	9.6e-06	Leucine rich repeat
Pkinase	PF00069	1022 - 1289	6.2e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	1023 - 1290	1.5e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012974)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07280
fragaria_vesca	FvH4_7g29180
malus_domestica	MD01G1198500.v1.1 MD07G1266400.v1.1
prunus_persica	Prupe.2G292000_v2.0.a1
pyrus_communis	pycom01g20870 pycom07g24420
rosa_chinensis	RchiOBHm_Chr1g0376951
rosa_laevigata	RLG00000026557
rosa_multiflora	Rmu_sc0026264.1_g000001 Rmu_ssc0000155.1_g000035
rosa_roxburghii	Rroxscaffold_4G00281610
rosa_rugosa	Rorug01G0399800
rosa_samantha	Rh1AG417000 Rh1BG376400 Rh1CG390200 Rh1DG407100
rosa_wichuraiana	Rw1G036600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2013
AccB7I	CCANNNNNTGG	2 cut(s)	2409, 2543
AccBSI	CCGCTC	3 cut(s)	259, 1545, 3585
AccI	GTMKAC	1 cut(s)	3593
AccII	CGCG	5 cut(s)	419, 774, 793, 3384, 3770
AccIII	TCCGGA	1 cut(s)	1270
AclI	AACGTT	1 cut(s)	2988
AclWI	GGATC	7 cut(s)	1746, 2423, 2508, 2882, 2891, 3323, 3746
AcuI	CTGAAG	8 cut(s)	464, 599, 737, 1080, 1974, 2922, 3354, 3743
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	6 cut(s)	890, 1812, 2161, 2238, 3101, 3242
AflIII	ACRYGT	1 cut(s)	1903
AgeI	ACCGGT	1 cut(s)	1721
AhlI	ACTAGT	1 cut(s)	2927
AjnI	CCWGG	9 cut(s)	513, 585, 591, 1130, 2167, 2802, 2918, 3198, 3387
AjuI	GAANNNNNNNTTGG	4 cut(s)	2514, 2546, 3210, 3242
Alw21I	GWGCWC	6 cut(s)	228, 522, 1068, 1721, 2836, 3864
Alw26I	GTCTC	4 cut(s)	431, 3003, 3515, 3826
Alw44I	GTGCAC	1 cut(s)	1717
AlwI	GGATC	7 cut(s)	1746, 2423, 2508, 2882, 2891, 3323, 3746
AlwNI	CAGNNNCTG	1 cut(s)	3721
Ama87I	CYCGRG	3 cut(s)	1330, 3236, 3635
Aor13HI	TCCGGA	1 cut(s)	1270
ApaI	GGGCCC	1 cut(s)	2673
ApaLI	GTGCAC	1 cut(s)	1717
ArsI	GACNNNNNNTTYG	2 cut(s)	1334, 1366
AseI	ATTAAT	1 cut(s)	3882
AsiGI	ACCGGT	1 cut(s)	1721
Asp700I	GAANNNNTTC	2 cut(s)	1161, 1466
AspLEI	GCGC	2 cut(s)	1409, 3783
AsuC2I	CCSGG	4 cut(s)	469, 574, 1189, 1883
AsuII	TTCGAA	6 cut(s)	1460, 1502, 1529, 1556, 1676, 3776
AvaI	CYCGRG	3 cut(s)	1330, 3236, 3635
AvaII	GGWCC	6 cut(s)	301, 589, 730, 1262, 2028, 3665
BaeGI	GKGCMC	3 cut(s)	1478, 1721, 2673
BanII	GRGCYC	3 cut(s)	522, 1068, 2673
BauI	CACGAG	2 cut(s)	157, 2339
BbsI	GAAGAC	1 cut(s)	3471
Bbv12I	GWGCWC	6 cut(s)	228, 522, 1068, 1721, 2836, 3864
BbvCI	CCTCAGC	1 cut(s)	1226
BccI	CCATC	9 cut(s)	1507, 2171, 2294, 2444, 2865, 3074, 3113, 3751, 3781
BceAI	ACGGC	4 cut(s)	278, 820, 1036, 3113
BcgI	CGANNNNNNTGC	2 cut(s)	1006, 1040
BciT130I	CCWGG	9 cut(s)	515, 587, 593, 1132, 2169, 2804, 2920, 3200, 3389
BclI	TGATCA	2 cut(s)	1992, 3507
BcnI	CCSGG	4 cut(s)	469, 574, 1189, 1883
BcoDI	GTCTC	4 cut(s)	431, 3003, 3515, 3826
BcuI	ACTAGT	1 cut(s)	2927
BfaI	CTAG	4 cut(s)	995, 2297, 2928, 3341
BfmI	CTRYAG	3 cut(s)	651, 2862, 3613
BfuAI	ACCTGC	1 cut(s)	2013
BglI	GCCNNNNNGGC	2 cut(s)	210, 1132
BlpI	GCTNAGC	5 cut(s)	990, 1541, 1709, 1757, 1790
Bme18I	GGWCC	6 cut(s)	301, 589, 730, 1262, 2028, 3665
BmeT110I	CYCGRG	3 cut(s)	1330, 3236, 3635
BmrI	ACTGGG	2 cut(s)	196, 3355
BmsI	GCATC	4 cut(s)	1014, 1128, 2991, 3708
BmuI	ACTGGG	2 cut(s)	196, 3355
BpiI	GAAGAC	1 cut(s)	3471
BplI	GAGNNNNNCTC	2 cut(s)	243, 275
BpmI	CTGGAG	4 cut(s)	455, 508, 536, 2520
Bpu10I	CCTNAGC	1 cut(s)	1226
Bpu1102I	GCTNAGC	5 cut(s)	990, 1541, 1709, 1757, 1790
Bpu14I	TTCGAA	6 cut(s)	1460, 1502, 1529, 1556, 1676, 3776
BpuEI	CTTGAG	5 cut(s)	1261, 2452, 2582, 2999, 3848
BpuMI	CCSGG	4 cut(s)	469, 574, 1189, 1883
BsaBI	GATNNNNATC	2 cut(s)	496, 2499
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	1 cut(s)	431
BsaWI	WCCGGW	4 cut(s)	1201, 1270, 1721, 3662
BsaXI	ACNNNNNCTCC	4 cut(s)	293, 323, 377, 407
Bse118I	RCCGGY	4 cut(s)	1403, 1721, 2720, 2889
Bse1I	ACTGG	5 cut(s)	191, 385, 525, 2218, 3350
Bse3DI	GCAATG	2 cut(s)	1291, 1808
Bse8I	GATNNNNATC	2 cut(s)	496, 2499
BseAI	TCCGGA	1 cut(s)	1270
BseBI	CCWGG	9 cut(s)	515, 587, 593, 1132, 2169, 2804, 2920, 3200, 3389
BseGI	GGATG	3 cut(s)	1990, 2163, 3423
BseJI	GATNNNNATC	2 cut(s)	496, 2499
BseMI	GCAATG	2 cut(s)	1291, 1808
BseNI	ACTGG	5 cut(s)	191, 385, 525, 2218, 3350
BseRI	GAGGAG	3 cut(s)	145, 298, 3272
BseSI	GKGCMC	3 cut(s)	1478, 1721, 2673
BseYI	CCCAGC	2 cut(s)	487, 3832
BsgI	GTGCAG	1 cut(s)	3396
Bsh1236I	CGCG	5 cut(s)	419, 774, 793, 3384, 3770
BshTI	ACCGGT	1 cut(s)	1721
BsiHKAI	GWGCWC	6 cut(s)	228, 522, 1068, 1721, 2836, 3864
BsiHKCI	CYCGRG	3 cut(s)	1330, 3236, 3635
BslFI	GGGAC	4 cut(s)	34, 2014, 3180, 3362
BsmAI	GTCTC	4 cut(s)	431, 3003, 3515, 3826
BsmBI	CGTCTC	1 cut(s)	3826
BsmFI	GGGAC	4 cut(s)	34, 2014, 3180, 3362
Bso31I	GGTCTC	1 cut(s)	431
BsoBI	CYCGRG	3 cut(s)	1330, 3236, 3635
Bsp119I	TTCGAA	6 cut(s)	1460, 1502, 1529, 1556, 1676, 3776
Bsp120I	GGGCCC	1 cut(s)	2669
Bsp1286I	GDGCHC	8 cut(s)	228, 522, 1068, 1478, 1721, 2673, 2836, 3864
Bsp13I	TCCGGA	1 cut(s)	1270
Bsp1407I	TGTACA	1 cut(s)	3099
Bsp1720I	GCTNAGC	5 cut(s)	990, 1541, 1709, 1757, 1790
Bsp19I	CCATGG	2 cut(s)	660, 3403
BspEI	TCCGGA	1 cut(s)	1270
BspFNI	CGCG	5 cut(s)	419, 774, 793, 3384, 3770
BspMI	ACCTGC	1 cut(s)	2013
BspPI	GGATC	7 cut(s)	1746, 2423, 2508, 2882, 2891, 3323, 3746
BspQI	GCTCTTC	1 cut(s)	1056
BspT104I	TTCGAA	6 cut(s)	1460, 1502, 1529, 1556, 1676, 3776
BspTNI	GGTCTC	1 cut(s)	431
BsrBI	CCGCTC	3 cut(s)	259, 1545, 3585
BsrDI	GCAATG	2 cut(s)	1291, 1808
BsrFI	RCCGGY	4 cut(s)	1403, 1721, 2720, 2889
BsrGI	TGTACA	1 cut(s)	3099
BsrI	ACTGG	5 cut(s)	191, 385, 525, 2218, 3350
BssAI	RCCGGY	4 cut(s)	1403, 1721, 2720, 2889
BssNI	GRCGYC	1 cut(s)	5
BssSI	CACGAG	2 cut(s)	157, 2339
BssT1I	CCWWGG	5 cut(s)	660, 2024, 2521, 2542, 3403
Bst2BI	CACGAG	2 cut(s)	157, 2339
Bst2UI	CCWGG	9 cut(s)	515, 587, 593, 1132, 2169, 2804, 2920, 3200, 3389
Bst4CI	ACNGT	9 cut(s)	130, 223, 326, 702, 1004, 1970, 2736, 3097, 3245
Bst6I	CTCTTC	5 cut(s)	278, 624, 840, 1056, 1269
BstACI	GRCGYC	1 cut(s)	5
BstAPI	GCANNNNNTGC	2 cut(s)	2858, 3374
BstAUI	TGTACA	1 cut(s)	3099
BstBI	TTCGAA	6 cut(s)	1460, 1502, 1529, 1556, 1676, 3776
BstDSI	CCRYGG	3 cut(s)	660, 3382, 3403
BstEII	GGTNACC	1 cut(s)	383
BstENI	CCTNNNNNAGG	2 cut(s)	1930, 3203
BstF5I	GGATG	3 cut(s)	1990, 2163, 3423
BstFNI	CGCG	5 cut(s)	419, 774, 793, 3384, 3770
BstHHI	GCGC	2 cut(s)	1409, 3783
BstMAI	GTCTC	4 cut(s)	431, 3003, 3515, 3826
BstNI	CCWGG	9 cut(s)	515, 587, 593, 1132, 2169, 2804, 2920, 3200, 3389
BstNSI	RCATGY	3 cut(s)	1907, 2080, 2332
BstPI	GGTNACC	1 cut(s)	383
BstSFI	CTRYAG	3 cut(s)	651, 2862, 3613
BstSLI	GKGCMC	3 cut(s)	1478, 1721, 2673
BstUI	CGCG	5 cut(s)	419, 774, 793, 3384, 3770
BstV2I	GAAGAC	1 cut(s)	3471
BstX2I	RGATCY	3 cut(s)	1738, 2500, 3328
BstXI	CCANNNNNNTGG	1 cut(s)	2471
BstYI	RGATCY	3 cut(s)	1738, 2500, 3328
BtgI	CCRYGG	3 cut(s)	660, 3382, 3403
BtgZI	GCGATG	1 cut(s)	2301
BtsCI	GGATG	3 cut(s)	1990, 2163, 3423
BtsI	GCAGTG	1 cut(s)	178
BtsIMutI	CAGTG	6 cut(s)	178, 378, 1079, 1095, 1966, 2089
BveI	ACCTGC	1 cut(s)	2013
CaiI	CAGNNNCTG	1 cut(s)	3721
CfoI	GCGC	2 cut(s)	1409, 3783
Cfr10I	RCCGGY	4 cut(s)	1403, 1721, 2720, 2889
Cfr42I	CCGCGG	1 cut(s)	3385
CseI	GACGC	3 cut(s)	818, 3776, 3808
CsiI	ACCWGGT	1 cut(s)	2918
Csp6I	GTAC	6 cut(s)	889, 1811, 2160, 2237, 3100, 3241
CspAI	ACCGGT	1 cut(s)	1721
CviQI	GTAC	6 cut(s)	889, 1811, 2160, 2237, 3100, 3241
Eam1104I	CTCTTC	5 cut(s)	278, 624, 840, 1056, 1269
EarI	CTCTTC	5 cut(s)	278, 624, 840, 1056, 1269
EciI	GGCGGA	3 cut(s)	160, 1718, 2666
Ecl136II	GAGCTC	2 cut(s)	520, 1066
Eco130I	CCWWGG	5 cut(s)	660, 2024, 2521, 2542, 3403
Eco147I	AGGCCT	2 cut(s)	3339, 3844
Eco24I	GRGCYC	3 cut(s)	522, 1068, 2673
Eco31I	GGTCTC	1 cut(s)	431
Eco32I	GATATC	1 cut(s)	3436
Eco47I	GGWCC	6 cut(s)	301, 589, 730, 1262, 2028, 3665
Eco53kI	GAGCTC	2 cut(s)	520, 1066
Eco57I	CTGAAG	8 cut(s)	464, 599, 737, 1080, 1974, 2922, 3354, 3743
Eco88I	CYCGRG	3 cut(s)	1330, 3236, 3635
Eco91I	GGTNACC	1 cut(s)	383
EcoICRI	GAGCTC	2 cut(s)	520, 1066
EcoNI	CCTNNNNNAGG	2 cut(s)	1930, 3203
EcoO109I	RGGNCCY	3 cut(s)	2028, 2669, 2670
EcoO65I	GGTNACC	1 cut(s)	383
EcoRI	GAATTC	3 cut(s)	1498, 1672, 3176
EcoRII	CCWGG	9 cut(s)	513, 585, 591, 1130, 2167, 2802, 2918, 3198, 3387
EcoRV	GATATC	1 cut(s)	3436
EcoT14I	CCWWGG	5 cut(s)	660, 2024, 2521, 2542, 3403
EcoT38I	GRGCYC	3 cut(s)	522, 1068, 2673
ErhI	CCWWGG	5 cut(s)	660, 2024, 2521, 2542, 3403
Esp3I	CGTCTC	1 cut(s)	3826
FalI	AAGNNNNNCTT	6 cut(s)	99, 131, 2414, 2446, 2658, 2690
FaqI	GGGAC	4 cut(s)	34, 2014, 3180, 3362
FauI	CCCGC	9 cut(s)	86, 151, 264, 486, 779, 1282, 3389, 3578, 3683
FbaI	TGATCA	2 cut(s)	1992, 3507
FblI	GTMKAC	1 cut(s)	3593
FokI	GGATG	3 cut(s)	1997, 2150, 3410
FriOI	GRGCYC	3 cut(s)	522, 1068, 2673
FspBI	CTAG	4 cut(s)	995, 2297, 2928, 3341
GlaI	GCGC	2 cut(s)	1408, 3782
GsaI	CCCAGC	2 cut(s)	491, 3836
GsuI	CTGGAG	4 cut(s)	455, 508, 536, 2520
HgaI	GACGC	3 cut(s)	818, 3776, 3808
HhaI	GCGC	2 cut(s)	1409, 3783
Hin1I	GRCGYC	1 cut(s)	5
Hin6I	GCGC	2 cut(s)	1407, 3781
HinP1I	GCGC	2 cut(s)	1407, 3781
HincII	GTYRAC	5 cut(s)	426, 870, 1257, 2925, 3031
HindII	GTYRAC	5 cut(s)	426, 870, 1257, 2925, 3031
HindIII	AAGCTT	5 cut(s)	641, 2120, 2173, 2191, 2201
Hpy99I	CGWCG	2 cut(s)	10, 1572
HpyCH4III	ACNGT	9 cut(s)	130, 223, 326, 702, 1004, 1970, 2736, 3097, 3245
HpyCH4IV	ACGT	7 cut(s)	87, 603, 666, 1349, 2988, 3110, 3355
HpySE526I	ACGT	7 cut(s)	87, 603, 666, 1349, 2988, 3110, 3355
Hsp92I	GRCGYC	1 cut(s)	5
HspAI	GCGC	2 cut(s)	1407, 3781
Kpn2I	TCCGGA	1 cut(s)	1270
KroI	GCCGGC	2 cut(s)	1403, 2889
KroNI	GCCGGC	2 cut(s)	1405, 2891
Ksp22I	TGATCA	2 cut(s)	1992, 3507
KspI	CCGCGG	1 cut(s)	3385
LguI	GCTCTTC	1 cut(s)	1056
LmnI	GCTCC	9 cut(s)	264, 484, 517, 1429, 1705, 1777, 2971, 3270, 3294
LweI	GCATC	4 cut(s)	1014, 1128, 2991, 3708
MabI	ACCWGGT	1 cut(s)	2918
MaeI	CTAG	4 cut(s)	995, 2297, 2928, 3341
MaeII	ACGT	7 cut(s)	87, 603, 666, 1349, 2988, 3110, 3355
MaeIII	GTNAC	8 cut(s)	217, 383, 1230, 1601, 1964, 2582, 2723, 3643
MbiI	CCGCTC	3 cut(s)	259, 1545, 3585
MfeI	CAATTG	4 cut(s)	206, 705, 1338, 1796
MflI	RGATCY	3 cut(s)	1738, 2500, 3328
MhlI	GDGCHC	8 cut(s)	228, 522, 1068, 1478, 1721, 2673, 2836, 3864
MlyI	GAGTC	4 cut(s)	56, 225, 2817, 3517
MmeI	TCCRAC	6 cut(s)	309, 336, 575, 1888, 2643, 2806
MroI	TCCGGA	1 cut(s)	1270
MroNI	GCCGGC	2 cut(s)	1403, 2889
MroXI	GAANNNNTTC	2 cut(s)	1161, 1466
MseI	TTAA	8 cut(s)	972, 2042, 2205, 2282, 2370, 2796, 3138, 3882
MslI	CAYNNNNRTG	1 cut(s)	901
MspA1I	CMGCKG	3 cut(s)	1540, 1577, 3384
MunI	CAATTG	4 cut(s)	206, 705, 1338, 1796
MvaI	CCWGG	9 cut(s)	515, 587, 593, 1132, 2169, 2804, 2920, 3200, 3389
MvnI	CGCG	5 cut(s)	419, 774, 793, 3384, 3770
NaeI	GCCGGC	2 cut(s)	1405, 2891
NciI	CCSGG	4 cut(s)	469, 574, 1189, 1883
NcoI	CCATGG	2 cut(s)	660, 3403
NgoMIV	GCCGGC	2 cut(s)	1403, 2889
NmeAIII	GCCGAG	1 cut(s)	239
NmuCI	GTSAC	3 cut(s)	217, 1964, 3643
NspI	RCATGY	3 cut(s)	1907, 2080, 2332
NspV	TTCGAA	6 cut(s)	1460, 1502, 1529, 1556, 1676, 3776
PaeI	GCATGC	1 cut(s)	2332
PaeR7I	CTCGAG	1 cut(s)	3635
PasI	CCCWGGG	2 cut(s)	2803, 3199
PceI	AGGCCT	2 cut(s)	3339, 3844
PciI	ACATGT	1 cut(s)	1903
PciSI	GCTCTTC	1 cut(s)	1056
PcsI	WCGNNNNNNNCGW	2 cut(s)	69, 3782
PdiI	GCCGGC	2 cut(s)	1405, 2891
PdmI	GAANNNNTTC	2 cut(s)	1161, 1466
PflFI	GACNNNGTC	1 cut(s)	188
PflMI	CCANNNNNTGG	2 cut(s)	2409, 2543
PfoI	TCCNGGA	2 cut(s)	585, 1187
PinAI	ACCGGT	1 cut(s)	1721
PleI	GAGTC	4 cut(s)	55, 224, 2816, 3517
PpsI	GAGTC	4 cut(s)	55, 224, 2816, 3517
PpuMI	RGGWCCY	1 cut(s)	2028
PscI	ACATGT	1 cut(s)	1903
PshBI	ATTAAT	1 cut(s)	3882
Psp124BI	GAGCTC	2 cut(s)	522, 1068
Psp1406I	AACGTT	1 cut(s)	2988
Psp5II	RGGWCCY	1 cut(s)	2028
Psp6I	CCWGG	9 cut(s)	513, 585, 591, 1130, 2167, 2802, 2918, 3198, 3387
PspEI	GGTNACC	1 cut(s)	383
PspFI	CCCAGC	2 cut(s)	487, 3832
PspGI	CCWGG	9 cut(s)	513, 585, 591, 1130, 2167, 2802, 2918, 3198, 3387
PspOMI	GGGCCC	1 cut(s)	2669
PspPPI	RGGWCCY	1 cut(s)	2028
PspXI	VCTCGAGB	1 cut(s)	3635
PstNI	CAGNNNCTG	1 cut(s)	3721
PsuI	RGATCY	3 cut(s)	1738, 2500, 3328
PsyI	GACNNNGTC	1 cut(s)	188
PvuII	CAGCTG	2 cut(s)	1540, 1577
RsaI	GTAC	6 cut(s)	890, 1812, 2161, 2238, 3101, 3242
RsaNI	GTAC	6 cut(s)	889, 1811, 2160, 2237, 3100, 3241
RseI	CAYNNNNRTG	1 cut(s)	901
SacI	GAGCTC	2 cut(s)	522, 1068
SacII	CCGCGG	1 cut(s)	3385
SapI	GCTCTTC	1 cut(s)	1056
SaqAI	TTAA	8 cut(s)	972, 2042, 2205, 2282, 2370, 2796, 3138, 3882
SchI	GAGTC	4 cut(s)	56, 225, 2817, 3517
SduI	GDGCHC	8 cut(s)	228, 522, 1068, 1478, 1721, 2673, 2836, 3864
SexAI	ACCWGGT	1 cut(s)	2918
SfaNI	GCATC	4 cut(s)	1014, 1128, 2991, 3708
SfcI	CTRYAG	3 cut(s)	651, 2862, 3613
Sfr274I	CTCGAG	1 cut(s)	3635
Sfr303I	CCGCGG	1 cut(s)	3385
SfuI	TTCGAA	6 cut(s)	1460, 1502, 1529, 1556, 1676, 3776
SgrBI	CCGCGG	1 cut(s)	3385
SinI	GGWCC	6 cut(s)	301, 589, 730, 1262, 2028, 3665
SlaI	CTCGAG	1 cut(s)	3635
SmiMI	CAYNNNNRTG	1 cut(s)	901
SmlI	CTYRAG	6 cut(s)	1240, 2431, 2561, 3014, 3635, 3863
SmoI	CTYRAG	6 cut(s)	1240, 2431, 2561, 3014, 3635, 3863
SpeI	ACTAGT	1 cut(s)	2927
SphI	GCATGC	1 cut(s)	2332
SseBI	AGGCCT	2 cut(s)	3339, 3844
SspI	AATATT	1 cut(s)	1668
SspMI	CTAG	4 cut(s)	995, 2297, 2928, 3341
SstI	GAGCTC	2 cut(s)	522, 1068
StuI	AGGCCT	2 cut(s)	3339, 3844
StyI	CCWWGG	5 cut(s)	660, 2024, 2521, 2542, 3403
TaaI	ACNGT	9 cut(s)	130, 223, 326, 702, 1004, 1970, 2736, 3097, 3245
TaiI	ACGT	7 cut(s)	90, 606, 669, 1352, 2991, 3113, 3358
TatI	WGTACW	1 cut(s)	3099
TauI	GCSGC	5 cut(s)	166, 796, 1764, 3010, 3387
Tru1I	TTAA	8 cut(s)	972, 2042, 2205, 2282, 2370, 2796, 3138, 3882
Tru9I	TTAA	8 cut(s)	972, 2042, 2205, 2282, 2370, 2796, 3138, 3882
TscAI	CASTG	6 cut(s)	185, 385, 1086, 1102, 1973, 2089
TseFI	GTSAC	3 cut(s)	217, 1964, 3643
Tsp45I	GTSAC	3 cut(s)	217, 1964, 3643
TspDTI	ATGAA	9 cut(s)	25, 2304, 2441, 2495, 2657, 2796, 2923, 2925, 3169
TspGWI	ACGGA	4 cut(s)	118, 743, 1790, 2507
TspRI	CASTG	6 cut(s)	185, 385, 1086, 1102, 1973, 2089
Tth111I	GACNNNGTC	1 cut(s)	188
Van91I	CCANNNNNTGG	2 cut(s)	2409, 2543
VneI	GTGCAC	1 cut(s)	1717
VpaK11BI	GGWCC	6 cut(s)	301, 589, 730, 1262, 2028, 3665
VspI	ATTAAT	1 cut(s)	3882
XagI	CCTNNNNNAGG	2 cut(s)	1930, 3203
XceI	RCATGY	3 cut(s)	1907, 2080, 2332
XhoI	CTCGAG	1 cut(s)	3635
XmiI	GTMKAC	1 cut(s)	3593
XmnI	GAANNNNTTC	2 cut(s)	1161, 1466
XspI	CTAG	4 cut(s)	995, 2297, 2928, 3341

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.