Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0012974

Physical Location & Seq

Genomic Coordinates Reverse (-)

3791224 .. 3795042

Gene Structure 3819 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000026557

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3819 bp

ATGGAGTTGATAAAGTCTGTTCCTTTAGTGGTCTTAGCATTTGGGTGGCTTCTGATCACCACTGCAGCCATTGTTGAAGAAAACCCAGAAGCAAAAGCCCTCATTTCCTTCAAAATTGGCCTCGAAAACCCAGAAATTCTCAGCTCATGGAGAGGTTCCATTCCAGTCTGCAACTGGGTCGGCGTCTCATGCCAACTCGGCCGAGTCAACTCACTCTCCCTACCCAATTTGGGCCTCAGAGGCCCACTGCCTCCTTCTCTCTTCTCTCTCCCAGACCTCGCCGTCCTCGACCTCTCCTCCAACCTCCTCCGCGGCCCAATTCCGGCCCAACTCGGTGACCTGACTCAGCTGCAGACTCTCAAACTCAGCTCCAACTCGCTCGCCGGAAAAATCCCGCCGGAGCTCGGAAAATTGTCCCGGCTGCAGACTCTTGAGCTCTCCGGAAATGGGCTCGCCGGAGAAGTCCCGGCCCACGTTGGGAACTTGACCCGGCTTCAGTACTTGGACTTGGGTAACAACCTCCTCTCTGGTTCTCTGCCTTTAACTCTGTTTCAAAATCTTGAGTCTTTATCTTCATTGGACATATCAAACAATTCGTTTTCCGGTTTAATCCCACCGGAAATCGGTAACCTGAGAAACCTCACTGACCTCTACATTGGGGTGAACCGTTTTTCGGGTCCATTGCCTAAAGAAATCGGAAACCTTTCAATGCTGGAGAATTTTTTCTCTCCGGCGTGTTCTATAACCGGTCCGTTGCCGGAGGAGTTGTCCAAGCTGAAGTCTTTGAGTAAGCTTGATCTTTCTCACAACCCTTTGAGGTGTTCTATACCGAAAGAGATAGGGGAGTTGAGAGAGCTGAGTATATTGAACTTGGTGTTTTCTGAGCTCAGTGGGTTTGTCCCTGCCGAGCTGGGAAAGTGTAGGAATTTGAAGACTTTGATGCTTTCGTTTAATTCGCTTTCCGGGGAGTTGCCGGAGGAGCTTTCGGAGCTGCCAATGCTGACGTTTTCGGCTGAGAAGAACCAGCTTTCCGGGTCATTGCCGGCTTGGCTTGGGAAGTGGAGTCAGGCTGAGGCGATTTTGCTTTCGAGCAATCTGTTTTCAGGGAGGATTCCTCCTGAGATTGGGAATTGTTCTGTGCTCATGCATCTTAGCTTGAGCAACAACTTGTTGAGTGGTGCAATTCCAGAAGAGCTGTGCAATGCGGTTTCGCTTTTGGAGATTGATCTGGATAGTAATTTCCTTTCTGGAACAATTGAGAATACATTTGTGGGGTGTAGGAACCTTACTCAGTTGAATTTGGTGAATAATCAGATTGCTGGTTCGATTCCTGAGTACCTTTCGGAGCTTCCTCTGATGGCGATTGACCTTGATTCAAATAACTTCACCGGTGAATTACCTACTAGACTTTGGAGCTCTGCTAATTTGATGGAATTTTCGGCTGCTAATAATCAGTTGAAGGGTTCTCTCACCAAGGAGATTGGCAATGCTGCTGCATTGGAAAGGCTTGTTCTGAGTAACAACCAGTTGAAGGGCACCATTCCTAAGGAGATAGGGAATCTGAGTACTCTCTCTGTTCTGAACCTGAACTCTAATTTCCTTGAGGGGCAGATTCCGAGTGAGGTTAGCCTCTGCAAACTCACTACACTTGACTTGGGGAGCAACCATCTCAATGGCTCTATACCGGAGGAGCTTGCGGACCTCCCTGAGTTACAGTGTCTAGTCCTTTCCCACAATGATCTGTCCGGGCCAATACCTTCAAAGCCATCACTCTATTTTCGGCAGCTGACCATTCCTGATTTGAGCTTTGTTCAGCATGTTGGGGTCTTTGATCTCTCCTACAACAGATTGTCTGGCCCTATACCCGAAGACCTGGGCAGTTGTGTCGCTCTGGTTAATCTGTTGATCAGCAACAACATGCTCTCTGGTGGAGTTCCAAGATCACTCTCGCGCTTAACAAATCTCACAACCTTGGATTTATCTGGGAATATGCTTTCTGGTTCCATTCCTCCAGAGTTAGGTGATTCCCCCAAGCTTCAAGGTTTATATCTGGGAAATAACCAGCTTACAAGCACCATCCCAGCAAGCTTGGGTCGTTTGGGCAGTTTGGTGAAGCTGAATTTAACTGGTAATAAGTTATCAGGTGCTGTACCGACAAGTTTTGGGAACCTGAAAGAGTTAACTCACTTAGATTTGAGTTCTAATGAGCTGGATGGTGAGCTTCCTTCTTCTCTTTCAAGCATGCAGAATCTGGGAGGGTTGTACGTGCAAGAGAACAGGCTTTCTGGTCGAATAGATGAGTTGTTCTCGAACTCTATGGCTTGGAGGATTGAAACTGTCAACTTGAGCAACAACTTCCTTACTGGTGAATTGCCGCTTTCATTGGGAAATCTGTCATACTTGACATATTTGGATCTTCATGCAAATTTGTTTAGAGGAAAGATTCCTCCAGACCTTGGGAATCTGGTGCAACTCGAGTACATTGATGTTTCTAGTAACCAGCTATCTGGACAGATTCCTGAGAAAGTATGCAACCTCACCAGTCTGGTTTACTTGAATTTGGCAGAAAATGGTTTGGAAGGGTCGATTCCCAGTAGTGGAATTTGCAGGGACCTTTCAAAAGTATCTGTTGTTGGTAATAGAAAATTGTGTGGGAGAAGTTCGAATTTAGATTGCCGAGTCAAAAGCTTAGACAAATCAGCTGTGCTGAATGCCTGGGGACTCGCAGCAATTGTCGTTGGAAGTGCTCTGATCTCTCTTGCAGCGGTCGTGGGTCTGATCAGATGGGCTACAAGAAGTAGCCAGCAAAACGATCTTGAACAAAGCGAGGATAGCAAGCTCAAGAGTTTCATGGATCATCATCTCTATTTCCTGGGTAACAGCCAACCAAAGGAGCCTCTGAGCATCAATATGGCCATGTTTGAGCAGCCTCTTCTGAAACTAACTTTAGTGGATATCCTTGAAGCTACAAACAATTTCTGCAAGACAAACATTATTGGAGATGGAGGCTTTGGAACTGTGTACAAGGCCACTTTGTCCAATGGGAAAACAGTTGCAGTTAAGAAGCTGAGTGAGTATAAAACCCAGGGTCACCGAGAGTTCATTGCTGAAATGGAAACCCTGGGTAAGGTGAAGCACCAAAATCTCGTTTCATTGCTGGGATACTGTTCTATCAGTGAGGAGAAGCTCCTTGTTTATGAATACATGGTGAATGGGAGCCTAGACCTATGGCTGAGAAATCGGATGGGAGCTCTTGACGTCCTAGATTGGGACAGACGTTTCAAAATTGCAATGGGTGCAGCGCGAGGCCTATCATTTCTTCACCATGAACTAATCCCTCATATCATTCACAGGGATATTAAAGCCAGCAACATCTTACTTAACGAAGACTTTGAGCCAAAGGTTGCAGATTTTGGACTTGCAAGACTGATCAGTGACTGCGAGACTCATATCACAACTGATGTTGCCGGGACATTTGGCTACATTCCACCAGAGTATGGACAAAGTGGGAGGTCGACTAAAAAAGGAGATGTCTACAGTTTTGGTGTGATACTGCTTGAATTGGTGACCGGAAAAGAGCCAACTGGACCAGACTTCAAAGAGAAGGAAGGAGGAAATTTGGTCGGCTGGGTGCTTCAGAAGGTCAAGAAGGGGCAAGCTGCTGATGTTCTTGACCCCTTGGTACTCAGTGCGGATTCGAAGCAGGCCATGTTGCAGGTGCTGCAGATTGCTTGTGTGTGCCTTTGTGACAATCCTGCCAGCAGACCTACAATGCTTCAAGTGCTGAAGATCCTTAAAGGGATGAAAGATGATGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001708 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005575 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009556 GO:0009653 GO:0009791 GO:0009908 GO:0009987 GO:0010234 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019953 GO:0022402 GO:0022414 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0034293 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0043934 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044703 GO:0045165 GO:0046777 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048656 GO:0048657 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051704 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140096 GO:1901564 GO:1903046 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1273

Amino Acids

137.7

Weight (kDa)

5.23

Isoelectric Point (pI)

30.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	28 - 64	1e-08	Leucine rich repeat N-terminal domain
LRR_8	PF13855	83 - 127	6e-06	Leucine rich repeat
LRR_14	PF23598	85 - 221	6.8e-16	Leucine-rich repeat region
LRR_8	PF13855	164 - 223	1.4e-06	Leucine rich repeat
LRR_14	PF23598	210 - 328	2.6e-08	Leucine-rich repeat region
LRR_8	PF13855	381 - 439	9.1e-06	Leucine rich repeat
LRR_8	PF13855	653 - 713	3.4e-07	Leucine rich repeat
LRR_4	PF12799	653 - 691	5.8e-07	Leucine Rich repeats (2 copies)
LRR_8	PF13855	702 - 761	2.1e-07	Leucine rich repeat
LRR_14	PF23598	795 - 857	2e-06	Leucine-rich repeat region
Pkinase	PF00069	996 - 1263	4.7e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	997 - 1264	5.4e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0012974)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07280
fragaria_vesca	FvH4_7g29180
malus_domestica	MD01G1198500.v1.1 MD07G1266400.v1.1
prunus_persica	Prupe.2G292000_v2.0.a1
pyrus_communis	pycom01g20870 pycom07g24420
rosa_chinensis	RchiOBHm_Chr1g0376951
rosa_laevigata	RLG00000026557
rosa_multiflora	Rmu_sc0026264.1_g000001 Rmu_ssc0000155.1_g000035
rosa_roxburghii	Rroxscaffold_4G00281610
rosa_rugosa	Rorug01G0399800
rosa_samantha	Rh1AG417000 Rh1BG376400 Rh1CG390200 Rh1DG407100
rosa_wichuraiana	Rw1G036600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3706
AasI	GACNNNNNNGTC	1 cut(s)	281
AatII	GACGTC	1 cut(s)	3261
Acc36I	ACCTGC	1 cut(s)	3706
AccB1I	GGYRCC	1 cut(s)	1535
AccB7I	CCANNNNNTGG	2 cut(s)	2456, 3497
AccI	GTMKAC	2 cut(s)	3515, 3534
AccII	CGCG	3 cut(s)	312, 1951, 3304
AccIII	TCCGGA	1 cut(s)	440
AciI	CCGC	8 cut(s)	310, 312, 395, 1205, 1697, 2375, 2765, 3692
AclWI	GGATC	3 cut(s)	2421, 2862, 3784
AcoI	YGGCCR	2 cut(s)	199, 2913
AcuI	CTGAAG	4 cut(s)	479, 797, 3620, 3806
AcyI	GRCGYC	2 cut(s)	183, 3258
AfaI	GTAC	8 cut(s)	500, 1337, 1567, 2151, 2264, 2480, 3023, 3684
AgeI	ACCGGT	2 cut(s)	746, 1388
AjnI	CCWGG	5 cut(s)	1872, 2714, 2871, 3084, 3120
AloI	GAACNNNNNNTCC	2 cut(s)	1013, 1045
Alw21I	GWGCWC	7 cut(s)	405, 438, 888, 1143, 1418, 2749, 3253
Alw26I	GTCTC	2 cut(s)	190, 3437
AlwI	GGATC	3 cut(s)	2421, 2862, 3784
AlwNI	CAGNNNCTG	4 cut(s)	2147, 2251, 3438, 3721
Ama87I	CYCGRG	1 cut(s)	2474
Aor13HI	TCCGGA	1 cut(s)	440
AsiGI	ACCGGT	2 cut(s)	746, 1388
Asp700I	GAANNNNTTC	2 cut(s)	703, 722
AspLEI	GCGC	2 cut(s)	1953, 3304
AsuC2I	CCSGG	7 cut(s)	418, 467, 490, 964, 1033, 1747, 3469
AsuII	TTCGAA	2 cut(s)	2663, 3698
AvaI	CYCGRG	1 cut(s)	2474
AvaII	GGWCC	5 cut(s)	677, 749, 1699, 2611, 3587
AxyI	CCTNAGG	1 cut(s)	1545
BaeGI	GKGCMC	1 cut(s)	1538
BalI	TGGCCA	1 cut(s)	2915
BanI	GGYRCC	1 cut(s)	1535
BanII	GRGCYC	6 cut(s)	405, 438, 453, 888, 1418, 3253
BbsI	GAAGAC	3 cut(s)	938, 1875, 3393
Bbv12I	GWGCWC	7 cut(s)	405, 438, 888, 1143, 1418, 2749, 3253
BbvCI	CCTCAGC	1 cut(s)	1071
BccI	CCATC	9 cut(s)	1351, 1423, 1674, 1774, 2084, 2207, 2778, 2996, 3238
BceAI	ACGGC	1 cut(s)	266
BcgI	CGANNNNNNTGC	4 cut(s)	160, 194, 1867, 1901
BciT130I	CCWGG	5 cut(s)	1874, 2716, 2873, 3086, 3122
BciVI	GTATCC	1 cut(s)	3155
BclI	TGATCA	4 cut(s)	54, 1905, 2778, 3429
BcnI	CCSGG	7 cut(s)	418, 467, 490, 964, 1033, 1747, 3469
BcoDI	GTCTC	2 cut(s)	190, 3437
BfaI	CTAG	5 cut(s)	1404, 1721, 2493, 3221, 3263
BfmI	CTRYAG	5 cut(s)	63, 350, 422, 3535, 3722
BfuAI	ACCTGC	1 cut(s)	3706
BfuI	GTATCC	1 cut(s)	3155
BglI	GCCNNNNNGGC	3 cut(s)	198, 240, 1048
BmcAI	AGTACT	2 cut(s)	500, 1567
Bme18I	GGWCC	5 cut(s)	677, 749, 1699, 2611, 3587
BmeT110I	CYCGRG	1 cut(s)	2474
BmiI	GGNNCC	9 cut(s)	157, 678, 1283, 1537, 2002, 2168, 2612, 2895, 3218
BmrI	ACTGGG	2 cut(s)	184, 2586
BmsI	GCATC	3 cut(s)	930, 1156, 2913
BmuI	ACTGGG	2 cut(s)	184, 2586
BpiI	GAAGAC	3 cut(s)	938, 1875, 3393
BplI	GAGNNNNNCTC	4 cut(s)	1099, 1131, 2291, 2323
BpmI	CTGGAG	3 cut(s)	734, 1995, 2433
Bpu10I	CCTNAGC	1 cut(s)	1071
Bpu14I	TTCGAA	2 cut(s)	2663, 3698
BpuEI	CTTGAG	6 cut(s)	452, 581, 1177, 1622, 2365, 2825
BpuMI	CCSGG	7 cut(s)	418, 467, 490, 964, 1033, 1747, 3469
BsaAI	YACGTR	1 cut(s)	2266
BsaBI	GATNNNNATC	1 cut(s)	2859
BsaHI	GRCGYC	2 cut(s)	183, 3258
BsaWI	WCCGGW	7 cut(s)	440, 602, 616, 746, 1388, 1684, 3569
BsaXI	ACNNNNNCTCC	6 cut(s)	200, 230, 281, 311, 752, 782
Bse118I	RCCGGY	3 cut(s)	746, 1042, 1388
Bse1I	ACTGG	8 cut(s)	164, 179, 1525, 2131, 2368, 2541, 2592, 3589
Bse21I	CCTNAGG	1 cut(s)	1545
Bse3DI	GCAATG	7 cut(s)	680, 1037, 1207, 1492, 3102, 3152, 3297
Bse8I	GATNNNNATC	1 cut(s)	2859
BseAI	TCCGGA	1 cut(s)	440
BseBI	CCWGG	5 cut(s)	1874, 2716, 2873, 3086, 3122
BseGI	GGATG	4 cut(s)	2076, 2218, 3249, 3807
BseJI	GATNNNNATC	1 cut(s)	2859
BseMI	GCAATG	7 cut(s)	680, 1037, 1207, 1492, 3102, 3152, 3297
BseNI	ACTGG	8 cut(s)	164, 179, 1525, 2131, 2368, 2541, 2592, 3589
BseRI	GAGGAG	7 cut(s)	286, 296, 512, 776, 992, 1703, 3194
BseSI	GKGCMC	1 cut(s)	1538
BseX3I	CGGCCG	1 cut(s)	199
BseYI	CCCAGC	4 cut(s)	910, 2080, 3157, 3627
BsgI	GTGCAG	1 cut(s)	3318
Bsh1236I	CGCG	3 cut(s)	312, 1951, 3304
Bsh1285I	CGRYCG	2 cut(s)	202, 2769
BshNI	GGYRCC	1 cut(s)	1535
BshTI	ACCGGT	2 cut(s)	746, 1388
BsiEI	CGRYCG	2 cut(s)	202, 2769
BsiHKAI	GWGCWC	7 cut(s)	405, 438, 888, 1143, 1418, 2749, 3253
BsiHKCI	CYCGRG	1 cut(s)	2474
BslFI	GGGAC	7 cut(s)	400, 449, 884, 2624, 2733, 3284, 3484
BsmAI	GTCTC	2 cut(s)	190, 3437
BsmBI	CGTCTC	1 cut(s)	190
BsmFI	GGGAC	7 cut(s)	400, 449, 884, 2624, 2733, 3284, 3484
BsmI	GAATGC	1 cut(s)	2716
BsoBI	CYCGRG	1 cut(s)	2474
Bsp119I	TTCGAA	2 cut(s)	2663, 3698
Bsp1286I	GDGCHC	9 cut(s)	405, 438, 453, 888, 1143, 1418, 1538, 2749, 3253
Bsp13I	TCCGGA	1 cut(s)	440
Bsp1407I	TGTACA	1 cut(s)	3021
BspACI	CCGC	8 cut(s)	310, 312, 395, 1205, 1697, 2375, 2765, 3692
BspEI	TCCGGA	1 cut(s)	440
BspFNI	CGCG	3 cut(s)	312, 1951, 3304
BspLI	GGNNCC	9 cut(s)	157, 678, 1283, 1537, 2002, 2168, 2612, 2895, 3218
BspMAI	CTGCAG	4 cut(s)	67, 354, 426, 3726
BspMI	ACCTGC	1 cut(s)	3706
BspPI	GGATC	3 cut(s)	2421, 2862, 3784
BspQI	GCTCTTC	1 cut(s)	1185
BspT104I	TTCGAA	2 cut(s)	2663, 3698
BspT107I	GGYRCC	1 cut(s)	1535
BsrDI	GCAATG	7 cut(s)	680, 1037, 1207, 1492, 3102, 3152, 3297
BsrFI	RCCGGY	3 cut(s)	746, 1042, 1388
BsrGI	TGTACA	1 cut(s)	3021
BsrI	ACTGG	8 cut(s)	164, 179, 1525, 2131, 2368, 2541, 2592, 3589
BssAI	RCCGGY	3 cut(s)	746, 1042, 1388
BssNI	GRCGYC	2 cut(s)	183, 3258
BssT1I	CCWWGG	4 cut(s)	1473, 1971, 2455, 3678
Bst2UI	CCWGG	5 cut(s)	1874, 2716, 2873, 3086, 3122
Bst4CI	ACNGT	7 cut(s)	668, 1716, 2338, 3019, 3052, 3167, 3539
Bst6I	CTCTTC	3 cut(s)	266, 1185, 2937
BstACI	GRCGYC	2 cut(s)	183, 3258
BstAPI	GCANNNNNTGC	2 cut(s)	3296, 3721
BstAUI	TGTACA	1 cut(s)	3021
BstBAI	YACGTR	1 cut(s)	2266
BstBI	TTCGAA	2 cut(s)	2663, 3698
BstDSI	CCRYGG	1 cut(s)	310
BstEII	GGTNACC	4 cut(s)	335, 626, 3089, 3565
BstENI	CCTNNNNNAGG	1 cut(s)	3125
BstF5I	GGATG	4 cut(s)	2076, 2218, 3249, 3807
BstFNI	CGCG	3 cut(s)	312, 1951, 3304
BstHHI	GCGC	2 cut(s)	1953, 3304
BstMAI	GTCTC	2 cut(s)	190, 3437
BstMCI	CGRYCG	2 cut(s)	202, 2769
BstNI	CCWGG	5 cut(s)	1874, 2716, 2873, 3086, 3122
BstNSI	RCATGY	3 cut(s)	1820, 1921, 2245
BstPI	GGTNACC	4 cut(s)	335, 626, 3089, 3565
BstSFI	CTRYAG	5 cut(s)	63, 350, 422, 3535, 3722
BstSLI	GKGCMC	1 cut(s)	1538
BstUI	CGCG	3 cut(s)	312, 1951, 3304
BstV2I	GAAGAC	3 cut(s)	938, 1875, 3393
BstX2I	RGATCY	2 cut(s)	2413, 3789
BstXI	CCANNNNNNTGG	2 cut(s)	1673, 2507
BstYI	RGATCY	2 cut(s)	2413, 3789
BstZI	CGGCCG	1 cut(s)	199
Bsu36I	CCTNAGG	1 cut(s)	1545
BsuI	GTATCC	1 cut(s)	3155
BtgI	CCRYGG	1 cut(s)	310
BtsCI	GGATG	4 cut(s)	2076, 2218, 3249, 3807
BtsI	GCAGTG	2 cut(s)	60, 245
BtsIMutI	CAGTG	8 cut(s)	60, 245, 642, 895, 1721, 3181, 3439, 3694
BveI	ACCTGC	1 cut(s)	3706
CaiI	CAGNNNCTG	4 cut(s)	2147, 2251, 3438, 3721
CfoI	GCGC	2 cut(s)	1953, 3304
Cfr10I	RCCGGY	3 cut(s)	746, 1042, 1388
Cfr42I	CCGCGG	1 cut(s)	313
CpoI	CGGWCCG	1 cut(s)	749
CseI	GACGC	1 cut(s)	172
Csp6I	GTAC	8 cut(s)	499, 1336, 1566, 2150, 2263, 2479, 3022, 3683
CspAI	ACCGGT	2 cut(s)	746, 1388
CspI	CGGWCCG	1 cut(s)	749
CviQI	GTAC	8 cut(s)	499, 1336, 1566, 2150, 2263, 2479, 3022, 3683
DrdI	GACNNNNNNGTC	1 cut(s)	281
DseDI	GACNNNNNNGTC	1 cut(s)	281
EaeI	YGGCCR	2 cut(s)	199, 2913
EagI	CGGCCG	1 cut(s)	199
Eam1104I	CTCTTC	3 cut(s)	266, 1185, 2937
EarI	CTCTTC	3 cut(s)	266, 1185, 2937
Ecl136II	GAGCTC	5 cut(s)	403, 436, 886, 1416, 3251
EclXI	CGGCCG	1 cut(s)	199
Eco130I	CCWWGG	4 cut(s)	1473, 1971, 2455, 3678
Eco147I	AGGCCT	1 cut(s)	3309
Eco24I	GRGCYC	6 cut(s)	405, 438, 453, 888, 1418, 3253
Eco32I	GATATC	1 cut(s)	2956
Eco47I	GGWCC	5 cut(s)	677, 749, 1699, 2611, 3587
Eco52I	CGGCCG	1 cut(s)	199
Eco53kI	GAGCTC	5 cut(s)	403, 436, 886, 1416, 3251
Eco57I	CTGAAG	4 cut(s)	479, 797, 3620, 3806
Eco81I	CCTNAGG	1 cut(s)	1545
Eco88I	CYCGRG	1 cut(s)	2474
Eco91I	GGTNACC	4 cut(s)	335, 626, 3089, 3565
EcoICRI	GAGCTC	5 cut(s)	403, 436, 886, 1416, 3251
EcoNI	CCTNNNNNAGG	1 cut(s)	3125
EcoO109I	RGGNCCY	1 cut(s)	2611
EcoO65I	GGTNACC	4 cut(s)	335, 626, 3089, 3565
EcoRII	CCWGG	5 cut(s)	1872, 2714, 2871, 3084, 3120
EcoRV	GATATC	1 cut(s)	2956
EcoT14I	CCWWGG	4 cut(s)	1473, 1971, 2455, 3678
EcoT22I	ATGCAT	1 cut(s)	1149
EcoT38I	GRGCYC	6 cut(s)	405, 438, 453, 888, 1418, 3253
ErhI	CCWWGG	4 cut(s)	1473, 1971, 2455, 3678
Esp3I	CGTCTC	1 cut(s)	190
FaqI	GGGAC	7 cut(s)	400, 449, 884, 2624, 2733, 3284, 3484
FauI	CCCGC	1 cut(s)	402
FbaI	TGATCA	4 cut(s)	54, 1905, 2778, 3429
FblI	GTMKAC	2 cut(s)	3515, 3534
FokI	GGATG	4 cut(s)	2063, 2225, 3256, 3814
FriOI	GRGCYC	6 cut(s)	405, 438, 453, 888, 1418, 3253
FspBI	CTAG	5 cut(s)	1404, 1721, 2493, 3221, 3263
GlaI	GCGC	2 cut(s)	1952, 3303
GsaI	CCCAGC	4 cut(s)	914, 2084, 3161, 3631
GsuI	CTGGAG	3 cut(s)	734, 1995, 2433
HgaI	GACGC	1 cut(s)	172
HhaI	GCGC	2 cut(s)	1953, 3304
Hin1I	GRCGYC	2 cut(s)	183, 3258
Hin6I	GCGC	2 cut(s)	1951, 3302
HinP1I	GCGC	2 cut(s)	1951, 3302
HincII	GTYRAC	4 cut(s)	208, 2181, 2341, 3516
HindII	GTYRAC	4 cut(s)	208, 2181, 2341, 3516
HindIII	AAGCTT	4 cut(s)	791, 2033, 2086, 2686
HpaI	GTTAAC	1 cut(s)	2181
Hpy166II	GTNNAC	8 cut(s)	208, 664, 2181, 2341, 2551, 3022, 3516, 3535
Hpy8I	GTNNAC	8 cut(s)	208, 664, 2181, 2341, 2551, 3022, 3516, 3535
HpyCH4III	ACNGT	7 cut(s)	668, 1716, 2338, 3019, 3052, 3167, 3539
HpyCH4IV	ACGT	5 cut(s)	474, 1004, 2265, 3258, 3277
HpySE526I	ACGT	5 cut(s)	474, 1004, 2265, 3258, 3277
Hsp92I	GRCGYC	2 cut(s)	183, 3258
HspAI	GCGC	2 cut(s)	1951, 3302
Kpn2I	TCCGGA	1 cut(s)	440
KroI	GCCGGC	1 cut(s)	1042
KroNI	GCCGGC	1 cut(s)	1044
Ksp22I	TGATCA	4 cut(s)	54, 1905, 2778, 3429
KspAI	GTTAAC	1 cut(s)	2181
KspI	CCGCGG	1 cut(s)	313
LguI	GCTCTTC	1 cut(s)	1185
LweI	GCATC	3 cut(s)	930, 1156, 2913
MaeI	CTAG	5 cut(s)	1404, 1721, 2493, 3221, 3263
MaeII	ACGT	5 cut(s)	474, 1004, 2265, 3258, 3277
MfeI	CAATTG	2 cut(s)	1254, 2730
MflI	RGATCY	2 cut(s)	2413, 3789
MhlI	GDGCHC	9 cut(s)	405, 438, 453, 888, 1143, 1418, 1538, 2749, 3253
MlsI	TGGCCA	1 cut(s)	2915
MluNI	TGGCCA	1 cut(s)	2915
MlyI	GAGTC	9 cut(s)	213, 337, 349, 421, 572, 1072, 2688, 2715, 3439
MmeI	TCCRAC	3 cut(s)	324, 396, 2719
Mox20I	TGGCCA	1 cut(s)	2915
Mph1103I	ATGCAT	1 cut(s)	1149
MroI	TCCGGA	1 cut(s)	440
MroNI	GCCGGC	1 cut(s)	1042
MroXI	GAANNNNTTC	2 cut(s)	703, 722
MscI	TGGCCA	1 cut(s)	2915
MslI	CAYNNNNRTG	4 cut(s)	43, 659, 1671, 2909
Msp20I	TGGCCA	1 cut(s)	2915
MspA1I	CMGCKG	5 cut(s)	312, 349, 1786, 2702, 2765
MunI	CAATTG	2 cut(s)	1254, 2730
Mva1269I	GAATGC	1 cut(s)	2716
MvaI	CCWGG	5 cut(s)	1874, 2716, 2873, 3086, 3122
MvnI	CGCG	3 cut(s)	312, 1951, 3304
NaeI	GCCGGC	1 cut(s)	1044
NciI	CCSGG	7 cut(s)	418, 467, 490, 964, 1033, 1747, 3469
NgoMIV	GCCGGC	1 cut(s)	1042
NlaIV	GGNNCC	9 cut(s)	157, 678, 1283, 1537, 2002, 2168, 2612, 2895, 3218
NmeAIII	GCCGAG	4 cut(s)	177, 227, 931, 2702
NmuCI	GTSAC	5 cut(s)	335, 3089, 3434, 3565, 3746
NsiI	ATGCAT	1 cut(s)	1149
NspI	RCATGY	3 cut(s)	1820, 1921, 2245
NspV	TTCGAA	2 cut(s)	2663, 3698
PaeI	GCATGC	1 cut(s)	2245
PaeR7I	CTCGAG	1 cut(s)	2474
PaqCI	CACCTGC	1 cut(s)	3706
PasI	CCCWGGG	2 cut(s)	3085, 3121
PceI	AGGCCT	1 cut(s)	3309
PciSI	GCTCTTC	1 cut(s)	1185
PcsI	WCGNNNNNNNCGW	1 cut(s)	285
PctI	GAATGC	1 cut(s)	2716
PdiI	GCCGGC	1 cut(s)	1044
PdmI	GAANNNNTTC	2 cut(s)	703, 722
PflMI	CCANNNNNTGG	2 cut(s)	2456, 3497
PinAI	ACCGGT	2 cut(s)	746, 1388
PleI	GAGTC	9 cut(s)	212, 337, 349, 421, 571, 1071, 2687, 2715, 3439
PpsI	GAGTC	9 cut(s)	212, 337, 349, 421, 571, 1071, 2687, 2715, 3439
Ppu21I	YACGTR	1 cut(s)	2266
PpuMI	RGGWCCY	1 cut(s)	2611
Psp124BI	GAGCTC	5 cut(s)	405, 438, 888, 1418, 3253
Psp5II	RGGWCCY	1 cut(s)	2611
Psp6I	CCWGG	5 cut(s)	1872, 2714, 2871, 3084, 3120
PspEI	GGTNACC	4 cut(s)	335, 626, 3089, 3565
PspFI	CCCAGC	4 cut(s)	910, 2080, 3157, 3627
PspGI	CCWGG	5 cut(s)	1872, 2714, 2871, 3084, 3120
PspN4I	GGNNCC	9 cut(s)	157, 678, 1283, 1537, 2002, 2168, 2612, 2895, 3218
PspPPI	RGGWCCY	1 cut(s)	2611
PspXI	VCTCGAGB	1 cut(s)	2474
PstI	CTGCAG	4 cut(s)	67, 354, 426, 3726
PstNI	CAGNNNCTG	4 cut(s)	2147, 2251, 3438, 3721
PsuI	RGATCY	2 cut(s)	2413, 3789
PvuII	CAGCTG	3 cut(s)	349, 1786, 2702
RsaI	GTAC	8 cut(s)	500, 1337, 1567, 2151, 2264, 2480, 3023, 3684
RsaNI	GTAC	8 cut(s)	499, 1336, 1566, 2150, 2263, 2479, 3022, 3683
RseI	CAYNNNNRTG	4 cut(s)	43, 659, 1671, 2909
Rsr2I	CGGWCCG	1 cut(s)	749
RsrII	CGGWCCG	1 cut(s)	749
SacI	GAGCTC	5 cut(s)	405, 438, 888, 1418, 3253
SacII	CCGCGG	1 cut(s)	313
SalI	GTCGAC	1 cut(s)	3514
SapI	GCTCTTC	1 cut(s)	1185
ScaI	AGTACT	2 cut(s)	500, 1567
SchI	GAGTC	9 cut(s)	213, 337, 349, 421, 572, 1072, 2688, 2715, 3439
SduI	GDGCHC	9 cut(s)	405, 438, 453, 888, 1143, 1418, 1538, 2749, 3253
SfaNI	GCATC	3 cut(s)	930, 1156, 2913
SfcI	CTRYAG	5 cut(s)	63, 350, 422, 3535, 3722
SfiI	GGCCNNNNNGGCC	1 cut(s)	240
Sfr274I	CTCGAG	1 cut(s)	2474
Sfr303I	CCGCGG	1 cut(s)	313
SfuI	TTCGAA	2 cut(s)	2663, 3698
SgrAI	CRCCGGYG	1 cut(s)	1388
SgrBI	CCGCGG	1 cut(s)	313
SinI	GGWCC	5 cut(s)	677, 749, 1699, 2611, 3587
SlaI	CTCGAG	1 cut(s)	2474
SmiMI	CAYNNNNRTG	4 cut(s)	43, 659, 1671, 2909
SmlI	CTYRAG	7 cut(s)	431, 560, 1156, 1601, 2344, 2474, 2840
SmoI	CTYRAG	7 cut(s)	431, 560, 1156, 1601, 2344, 2474, 2840
SphI	GCATGC	1 cut(s)	2245
SseBI	AGGCCT	1 cut(s)	3309
SsiI	CCGC	8 cut(s)	310, 312, 395, 1205, 1697, 2375, 2765, 3692
SspMI	CTAG	5 cut(s)	1404, 1721, 2493, 3221, 3263
SstI	GAGCTC	5 cut(s)	405, 438, 888, 1418, 3253
StuI	AGGCCT	1 cut(s)	3309
StyI	CCWWGG	4 cut(s)	1473, 1971, 2455, 3678
TaaI	ACNGT	7 cut(s)	668, 1716, 2338, 3019, 3052, 3167, 3539
TaiI	ACGT	5 cut(s)	477, 1007, 2268, 3261, 3280
TatI	WGTACW	4 cut(s)	498, 1565, 2478, 3021
TauI	GCSGC	2 cut(s)	315, 2377
TscAI	CASTG	8 cut(s)	67, 252, 649, 895, 1721, 3181, 3439, 3694
TseFI	GTSAC	5 cut(s)	335, 3089, 3434, 3565, 3746
Tsp45I	GTSAC	5 cut(s)	335, 3089, 3434, 3565, 3746
TspDTI	ATGAA	9 cut(s)	564, 2370, 2408, 2839, 3091, 3141, 3213, 3342, 3818
TspGWI	ACGGA	1 cut(s)	741
TspRI	CASTG	8 cut(s)	67, 252, 649, 895, 1721, 3181, 3439, 3694
Van91I	CCANNNNNTGG	2 cut(s)	2456, 3497
VpaK11BI	GGWCC	5 cut(s)	677, 749, 1699, 2611, 3587
XagI	CCTNNNNNAGG	1 cut(s)	3125
XceI	RCATGY	3 cut(s)	1820, 1921, 2245
XcmI	CCANNNNNNNNNTGG	1 cut(s)	171
XhoI	CTCGAG	1 cut(s)	2474
XmiI	GTMKAC	2 cut(s)	3515, 3534
XmnI	GAANNNNTTC	2 cut(s)	703, 722
XspI	CTAG	5 cut(s)	1404, 1721, 2493, 3221, 3263
ZraI	GACGTC	1 cut(s)	3259
ZrmI	AGTACT	2 cut(s)	500, 1567
Zsp2I	ATGCAT	1 cut(s)	1149

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.