FvH4_7g31160

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0008155
Physical Location & Seq
Reverse (-)
22550599 .. 22556594
5996 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g31160.t5

Sequence Viewer

Length: 1221 bp
ATGACGAACTTCGCTAAGCTGAACCTGCAGGCAATGGGCTTCCAGAGATCATCGACACCTGATAAGCAAAAGGTGCAACCTGTCAGTTCAAAAGTACTCAACAACGACGAGAATATTGGTGATCTTACCTTGGCATCAATAGCCTCAAACCTGCATGATATTTCCGCCATTGTTAATACACCCATCCCCTCATCAGATTTAGCTTACAGTTCATGGGTGGTGGAGCACCCTTCTGCACTAGGCTCATTTGATAGGATGATGAAAGCTGCAAAAGGGAAGAGGATTGTTGTCTTTTTAGACTATGACGGCACTCTCTCACCGATTGTTGATGATCCGGATCGTGCTTTCATGTCCAGTGAGATGAGAGCAGCAGTGCGCGAAGTTGCCAAATATTTTCCAACAGCAGTAATCAGCGGGAGGAGTAGAGACAAGGTCAAAGGATTTGTACAGTTAAGCAATGTATATTATGCCGGCAGCCACGGGATGGACATTATGGTCCCCTCAAAGCCGCTAAAGCCCTGTGATGCCAAGAACCAAGCCACAGCCTCAACGTTGGATAAGGAGAGTGACATGCTCTTTCAACCTGCCAAAAGATTCCTGCCTGCAATCCAAGAGATACGCATAGTGTTGGATGAAATAGCAAGAAAGATTGAAGGTGCAAGAGTAGAGGACAATAGATTCTGCATCAGTGTACATTACCGGAATGTCCGGGAAGAGGATTATGACATATTAGAAGAAAAGGTGAAGGCTGTGATTGAAACCTATCCTGAGTTTCACCTGACTCGGGGTAAAAAGGTCCTGGAGGTACGACCATCTATAGAATGGAACAAAGGGCATGCGCTTGAATATCTACTTGACACCCTTGGATTCAGTAACTCAGGTGACGTCCTCCCATTGTACATAGGGGATGATCGAACAGATGAAGATGCTTTCAAGGTGATAGAAGCCAGAGGACAGGGATTTTCTATAATCGTGTCCTCCACCCCAAAGGAAACCAAAGCTGCTTATTCTCTGCACGATCCATCGGAAGTGTTGACATTCTTATTACGCCTTGCAAGGTGGAGAAAAGCCTCGTCTTCAAGCAGGTCACTAGCTCAAATTTGGGGGGTCTTGGGAATTCACATTCACATAAGGCCAATTGCTAGGTTTTGGTACATAAAATTTCAAGAATTAGGAAGATTGAGGGGCAAGTGTGGTTATTTGTTACAGCTTGCCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Trehalose_PPase

Protein Analysis

407

Amino Acids

45.6

Weight (kDa)

8.72

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 109 - 353 1.3e-62 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 494
AatII GACGTC 1 cut(s) 888
Acc36I ACCTGC 4 cut(s) 33, 159, 592, 1074
AccB7I CCANNNNNTGG 1 cut(s) 484
AccII CGCG 1 cut(s) 378
AccIII TCCGGA 1 cut(s) 334
AciI CCGC 3 cut(s) 165, 414, 509
AclI AACGTT 1 cut(s) 551
AclWI GGATC 3 cut(s) 326, 345, 1013
AcsI RAATTY 3 cut(s) 1098, 1116, 1160
AcyI GRCGYC 1 cut(s) 885
AfaI GTAC 6 cut(s) 96, 447, 693, 807, 899, 1154
AfiI CCNNNNNNNGG 3 cut(s) 484, 715, 784
AgsI TTSAA 8 cut(s) 90, 581, 653, 758, 845, 934, 1080, 1166
AjnI CCWGG 1 cut(s) 798
AluBI AGCT 6 cut(s) 19, 203, 266, 1001, 1094, 1210
AluI AGCT 6 cut(s) 19, 203, 266, 1001, 1094, 1210
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 1 cut(s) 420
AlwI GGATC 3 cut(s) 326, 345, 1013
Ama87I CYCGRG 1 cut(s) 783
Aor13HI TCCGGA 1 cut(s) 334
AoxI GGCC 1 cut(s) 1133
ApeKI GCWGC 4 cut(s) 266, 368, 474, 1001
ApoI RAATTY 3 cut(s) 1098, 1116, 1160
AspLEI GCGC 2 cut(s) 378, 841
AspS9I GGNCC 2 cut(s) 496, 796
AsuC2I CCSGG 1 cut(s) 710
AsuHPI GGTGA 6 cut(s) 131, 309, 754, 767, 893, 949
AvaI CYCGRG 1 cut(s) 783
AvaII GGWCC 2 cut(s) 496, 796
BbsI GAAGAC 1 cut(s) 1068
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 4 cut(s) 253, 380, 486, 988
BccI CCATC 4 cut(s) 191, 478, 820, 1030
BceAI ACGGC 1 cut(s) 322
BciT130I CCWGG 1 cut(s) 800
BcnI CCSGG 1 cut(s) 710
BcoDI GTCTC 1 cut(s) 420
BfaI CTAG 3 cut(s) 239, 1091, 1143
BfmI CTRYAG 2 cut(s) 26, 816
BfuAI ACCTGC 4 cut(s) 33, 159, 592, 1074
BisI GCNGC 5 cut(s) 267, 369, 475, 509, 1002
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 5 cut(s) 268, 370, 476, 510, 1003
BmcAI AGTACT 1 cut(s) 96
Bme1390I CCNGG 2 cut(s) 710, 800
Bme18I GGWCC 2 cut(s) 496, 796
BmeT110I CYCGRG 1 cut(s) 783
BmgT120I GGNCC 2 cut(s) 496, 796
BmiI GGNNCC 1 cut(s) 498
BmrFI CCNGG 2 cut(s) 710, 800
BmsI GCATC 4 cut(s) 143, 514, 693, 916
BpiI GAAGAC 1 cut(s) 1068
BpmI CTGGAG 1 cut(s) 821
Bpu1102I GCTNAGC 1 cut(s) 15
BpuMI CCSGG 1 cut(s) 710
BsaBI GATNNNNATC 1 cut(s) 336
BsaHI GRCGYC 1 cut(s) 885
BsaJI CCNNGG 3 cut(s) 129, 478, 862
BsaWI WCCGGW 2 cut(s) 334, 699
Bsc4I CCNNNNNNNGG 3 cut(s) 484, 715, 784
Bse118I RCCGGY 1 cut(s) 470
Bse1I ACTGG 1 cut(s) 354
Bse3DI GCAATG 2 cut(s) 39, 463
Bse8I GATNNNNATC 1 cut(s) 336
BseAI TCCGGA 1 cut(s) 334
BseBI CCWGG 1 cut(s) 800
BseDI CCNNGG 3 cut(s) 129, 478, 862
BseGI GGATG 5 cut(s) 183, 261, 489, 637, 913
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 3 cut(s) 484, 715, 784
BseMI GCAATG 2 cut(s) 39, 463
BseMII CTCAG 2 cut(s) 759, 891
BseNI ACTGG 1 cut(s) 354
BseRI GAGGAG 1 cut(s) 433
BseXI GCAGC 4 cut(s) 253, 380, 486, 988
BsgI GTGCAG 2 cut(s) 219, 998
Bsh1236I CGCG 1 cut(s) 378
BshFI GGCC 1 cut(s) 1135
BsiHKAI GWGCWC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 783
BsiSI CCGG 4 cut(s) 335, 471, 700, 709
BslFI GGGAC 1 cut(s) 482
BslI CCNNNNNNNGG 3 cut(s) 484, 715, 784
BsmAI GTCTC 1 cut(s) 420
BsmFI GGGAC 1 cut(s) 482
BsnI GGCC 1 cut(s) 1135
BsoBI CYCGRG 1 cut(s) 783
Bsp1286I GDGCHC 1 cut(s) 228
Bsp13I TCCGGA 1 cut(s) 334
Bsp1407I TGTACA 3 cut(s) 445, 691, 897
Bsp143I GATC 6 cut(s) 47, 121, 331, 337, 910, 1018
Bsp1720I GCTNAGC 1 cut(s) 15
BspACI CCGC 3 cut(s) 165, 414, 509
BspANI GGCC 1 cut(s) 1135
BspCNI CTCAG 2 cut(s) 760, 890
BspEI TCCGGA 1 cut(s) 334
BspFNI CGCG 1 cut(s) 378
BspLI GGNNCC 1 cut(s) 498
BspMAI CTGCAG 1 cut(s) 30
BspMI ACCTGC 4 cut(s) 33, 159, 592, 1074
BspPI GGATC 3 cut(s) 326, 345, 1013
BsrDI GCAATG 2 cut(s) 39, 463
BsrFI RCCGGY 1 cut(s) 470
BsrGI TGTACA 3 cut(s) 445, 691, 897
BsrI ACTGG 1 cut(s) 354
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 3 cut(s) 129, 478, 862
BssMI GATC 6 cut(s) 47, 121, 331, 337, 910, 1018
BssNI GRCGYC 1 cut(s) 885
BssT1I CCWWGG 2 cut(s) 129, 862
Bst2UI CCWGG 1 cut(s) 800
Bst4CI ACNGT 2 cut(s) 209, 450
Bst6I CTCTTC 2 cut(s) 272, 708
BstACI GRCGYC 1 cut(s) 885
BstAPI GCANNNNNTGC 1 cut(s) 73
BstAUI TGTACA 3 cut(s) 445, 691, 897
BstC8I GCNNGC 5 cut(s) 30, 472, 603, 837, 1212
BstDEI CTNAG 3 cut(s) 15, 768, 877
BstDSI CCRYGG 1 cut(s) 478
BstF5I GGATG 5 cut(s) 183, 261, 489, 637, 913
BstFNI CGCG 1 cut(s) 378
BstHHI GCGC 2 cut(s) 378, 841
BstKTI GATC 6 cut(s) 50, 124, 334, 340, 913, 1021
BstMAI GTCTC 1 cut(s) 420
BstMBI GATC 6 cut(s) 47, 121, 331, 337, 910, 1018
BstMWI GCNNNNNNNGC 4 cut(s) 25, 73, 140, 514
BstNI CCWGG 1 cut(s) 800
BstNSI RCATGY 2 cut(s) 574, 839
BstSCI CCNGG 2 cut(s) 708, 798
BstSFI CTRYAG 2 cut(s) 26, 816
BstUI CGCG 1 cut(s) 378
BstV1I GCAGC 4 cut(s) 253, 380, 486, 988
BstV2I GAAGAC 1 cut(s) 1068
BsuRI GGCC 1 cut(s) 1135
BtgI CCRYGG 1 cut(s) 478
BtsCI GGATG 5 cut(s) 183, 261, 489, 637, 913
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 3 cut(s) 361, 378, 694
BveI ACCTGC 4 cut(s) 33, 159, 592, 1074
Cac8I GCNNGC 5 cut(s) 30, 472, 603, 837, 1212
CfoI GCGC 2 cut(s) 378, 841
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 2 cut(s) 496, 796
Csp6I GTAC 6 cut(s) 95, 446, 692, 806, 898, 1153
CviAII CATG 5 cut(s) 155, 213, 349, 571, 836
CviQI GTAC 6 cut(s) 95, 446, 692, 806, 898, 1153
DdeI CTNAG 3 cut(s) 15, 768, 877
DpnI GATC 6 cut(s) 49, 123, 333, 339, 912, 1020
DpnII GATC 6 cut(s) 47, 121, 331, 337, 910, 1018
DrdI GACNNNNNNGTC 1 cut(s) 494
DseDI GACNNNNNNGTC 1 cut(s) 494
Eam1104I CTCTTC 2 cut(s) 272, 708
EarI CTCTTC 2 cut(s) 272, 708
EciI GGCGGA 1 cut(s) 154
Eco130I CCWWGG 2 cut(s) 129, 862
Eco47I GGWCC 2 cut(s) 496, 796
Eco88I CYCGRG 1 cut(s) 783
EcoO109I RGGNCCY 1 cut(s) 796
EcoRI GAATTC 1 cut(s) 1116
EcoRII CCWGG 1 cut(s) 798
EcoT14I CCWWGG 2 cut(s) 129, 862
ErhI CCWWGG 2 cut(s) 129, 862
FaeI CATG 5 cut(s) 158, 216, 352, 574, 839
FaqI GGGAC 1 cut(s) 482
FatI CATG 5 cut(s) 154, 212, 348, 570, 835
FauI CCCGC 1 cut(s) 407
Fnu4HI GCNGC 5 cut(s) 267, 369, 475, 509, 1002
FokI GGATG 5 cut(s) 170, 268, 496, 644, 920
Fsp4HI GCNGC 5 cut(s) 267, 369, 475, 509, 1002
FspBI CTAG 3 cut(s) 239, 1091, 1143
GlaI GCGC 2 cut(s) 377, 840
GluI GCNGC 5 cut(s) 267, 369, 475, 509, 1002
GsuI CTGGAG 1 cut(s) 821
HaeIII GGCC 1 cut(s) 1135
HapII CCGG 4 cut(s) 335, 471, 700, 709
HhaI GCGC 2 cut(s) 378, 841
Hin1I GRCGYC 1 cut(s) 885
Hin1II CATG 5 cut(s) 158, 216, 352, 574, 839
Hin6I GCGC 2 cut(s) 376, 839
HinP1I GCGC 2 cut(s) 376, 839
HincII GTYRAC 1 cut(s) 1035
HindII GTYRAC 1 cut(s) 1035
HinfI GANTC 4 cut(s) 594, 678, 781, 867
HpaII CCGG 4 cut(s) 335, 471, 700, 709
HphI GGTGA 6 cut(s) 131, 309, 754, 767, 893, 949
Hpy166II GTNNAC 2 cut(s) 692, 1035
Hpy188I TCNGA 2 cut(s) 196, 1027
Hpy188III TCNNGA 4 cut(s) 43, 335, 767, 1166
Hpy8I GTNNAC 2 cut(s) 692, 1035
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 3 cut(s) 240, 647, 739
HpyCH4III ACNGT 2 cut(s) 209, 450
HpyCH4IV ACGT 2 cut(s) 551, 885
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 73, 140, 514
HpyF3I CTNAG 3 cut(s) 15, 768, 877
HpySE526I ACGT 2 cut(s) 551, 885
Hsp92I GRCGYC 1 cut(s) 885
Hsp92II CATG 5 cut(s) 158, 216, 352, 574, 839
HspAI GCGC 2 cut(s) 376, 839
Kpn2I TCCGGA 1 cut(s) 334
KroI GCCGGC 1 cut(s) 470
KroNI GCCGGC 1 cut(s) 472
Kzo9I GATC 6 cut(s) 47, 121, 331, 337, 910, 1018
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 4 cut(s) 253, 380, 486, 988
LweI GCATC 4 cut(s) 143, 514, 693, 916
MaeI CTAG 3 cut(s) 239, 1091, 1143
MaeII ACGT 2 cut(s) 551, 885
MaeIII GTNAC 5 cut(s) 566, 872, 881, 1086, 1203
MalI GATC 6 cut(s) 49, 123, 333, 339, 912, 1020
MboI GATC 6 cut(s) 47, 121, 331, 337, 910, 1018
MboII GAAGA 6 cut(s) 289, 725, 746, 935, 1068, 1188
MfeI CAATTG 1 cut(s) 1137
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 5 cut(s) 1098, 1116, 1137, 1160, 1169
MlyI GAGTC 1 cut(s) 775
MmeI TCCRAC 3 cut(s) 422, 534, 609
MroI TCCGGA 1 cut(s) 334
MroNI GCCGGC 1 cut(s) 470
MseI TTAA 2 cut(s) 174, 452
MspA1I CMGCKG 1 cut(s) 414
MspI CCGG 4 cut(s) 335, 471, 700, 709
MspR9I CCNGG 2 cut(s) 710, 800
MunI CAATTG 1 cut(s) 1137
MvaI CCWGG 1 cut(s) 800
MvnI CGCG 1 cut(s) 378
MwoI GCNNNNNNNGC 4 cut(s) 25, 73, 140, 514
NaeI GCCGGC 1 cut(s) 472
NciI CCSGG 1 cut(s) 710
NdeII GATC 6 cut(s) 47, 121, 331, 337, 910, 1018
NgoMIV GCCGGC 1 cut(s) 470
NlaIII CATG 5 cut(s) 158, 216, 352, 574, 839
NlaIV GGNNCC 1 cut(s) 498
NmuCI GTSAC 3 cut(s) 566, 881, 1086
NspI RCATGY 2 cut(s) 574, 839
PaeI GCATGC 1 cut(s) 839
PdiI GCCGGC 1 cut(s) 472
PfeI GAWTC 3 cut(s) 594, 678, 867
PflFI GACNNNGTC 1 cut(s) 431
PflMI CCANNNNNTGG 1 cut(s) 484
PfoI TCCNGGA 2 cut(s) 708, 798
PkrI GCNGC 5 cut(s) 268, 370, 476, 510, 1003
PleI GAGTC 1 cut(s) 775
PpsI GAGTC 1 cut(s) 775
PpuMI RGGWCCY 1 cut(s) 796
Psp1406I AACGTT 1 cut(s) 551
Psp5II RGGWCCY 1 cut(s) 796
Psp6I CCWGG 1 cut(s) 798
PspGI CCWGG 1 cut(s) 798
PspN4I GGNNCC 1 cut(s) 498
PspPI GGNCC 2 cut(s) 496, 796
PspPPI RGGWCCY 1 cut(s) 796
PstI CTGCAG 1 cut(s) 30
PsyI GACNNNGTC 1 cut(s) 431
RsaI GTAC 6 cut(s) 96, 447, 693, 807, 899, 1154
RsaNI GTAC 6 cut(s) 95, 446, 692, 806, 898, 1153
SaqAI TTAA 2 cut(s) 174, 452
SatI GCNGC 5 cut(s) 267, 369, 475, 509, 1002
Sau3AI GATC 6 cut(s) 47, 121, 331, 337, 910, 1018
Sau96I GGNCC 2 cut(s) 496, 796
SbfI CCTGCAGG 1 cut(s) 30
ScaI AGTACT 1 cut(s) 96
SchI GAGTC 1 cut(s) 775
ScrFI CCNGG 2 cut(s) 710, 800
SdaI CCTGCAGG 1 cut(s) 30
SduI GDGCHC 1 cut(s) 228
SfaNI GCATC 4 cut(s) 143, 514, 693, 916
SfcI CTRYAG 2 cut(s) 26, 816
SinI GGWCC 2 cut(s) 496, 796
SphI GCATGC 1 cut(s) 839
Sse8387I CCTGCAGG 1 cut(s) 30
Sse9I AATT 5 cut(s) 1098, 1116, 1137, 1160, 1169
SsiI CCGC 3 cut(s) 165, 414, 509
SspI AATATT 2 cut(s) 115, 392
SspMI CTAG 3 cut(s) 239, 1091, 1143
StyD4I CCNGG 2 cut(s) 708, 798
StyI CCWWGG 2 cut(s) 129, 862
TaaI ACNGT 2 cut(s) 209, 450
TaiI ACGT 2 cut(s) 554, 888
TaqI TCGA 2 cut(s) 53, 913
TasI AATT 5 cut(s) 1098, 1116, 1137, 1160, 1169
TatI WGTACW 4 cut(s) 94, 445, 691, 897
TauI GCSGC 1 cut(s) 511
TfiI GAWTC 3 cut(s) 594, 678, 867
Tru1I TTAA 2 cut(s) 174, 452
Tru9I TTAA 2 cut(s) 174, 452
TscAI CASTG 3 cut(s) 361, 378, 694
TseFI GTSAC 3 cut(s) 566, 881, 1086
TseI GCWGC 4 cut(s) 266, 368, 474, 1001
Tsp45I GTSAC 3 cut(s) 566, 881, 1086
TspDTI ATGAA 5 cut(s) 201, 275, 337, 648, 936
TspRI CASTG 3 cut(s) 361, 378, 694
Tth111I GACNNNGTC 1 cut(s) 431
Van91I CCANNNNNTGG 1 cut(s) 484
VpaK11BI GGWCC 2 cut(s) 496, 796
XapI RAATTY 3 cut(s) 1098, 1116, 1160
XceI RCATGY 2 cut(s) 574, 839
XcmI CCANNNNNNNNNTGG 1 cut(s) 819
XspI CTAG 3 cut(s) 239, 1091, 1143
ZraI GACGTC 1 cut(s) 886
ZrmI AGTACT 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.