MD07G1286100.v1.1

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008155
Physical Location & Seq
Reverse (-)
34815809 .. 34818454
2646 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1286100.v1.1.491

Sequence Viewer

Length: 1131 bp
ATGGTGATCATGACAAACTCGGCGAAGCTGAACCGAGCAATGGGACTCCATAGGTCATCAACTTCCAATAAGCAAAAGGTGCAACCCATCACGAAAGCAGTAAAGAATGACGAGAACAGTGTCAATCTTTCCTTAGCATCAATCACCTCAAACCTGCATGACATCTCTACCCCTAATAGACCAATTTCCTCAGATTTAGCTTATAATTCCTGGGTGGTTGAGCACCCTTCTGCATTGGGCTCATTTGATCAGATGATGAAAGCAGCAAAAGGGAAGAGGATGGTTGTTTTCCTAGACTACGATGGGACCCTTTCACCAATTGTTGATGATCCTGACCGTGCCTTCATGTCTGATGAGATGCGTGCTGCAGTGCGGGAAGTTGCCAAGTATTTTCCAACAGCAGTAATTAGTGGAAGGAGTAGAGATAAGGTAAAAGGATTTGTACAGTTAAGTAATGTATATTATGCTGGAAGCCATGGGATGGATATAATGGTCCCACCAAGGCCACTAAAGCCCTGCGATGGCAAGAACCACACTACAGCCATGGATAAAAATGCGAGTGACGTTCTCTTTCAACCTGCTAAAAGATTTCTGCCTGCAATCCAAGAGATACGAACACTGTTAGAGGAGATAACCAGGAAAATAGAAGGTGCTAGGGTAGAGGACAACAGATTCTGCCTCTCTGTACATTTCCGCAAGGTCCAGGATGAGGATTATGACATATTAGAAGCAAAGGTGAAGTCTGTACTTGAAAACTATCCTGAATTTCATCTCACCTTCGGCAAAAAGGTCTTGGAAGTACGGCCATCCATAGAATGGAACAAAGGTCATGCCCTAGAATATTTACTTGACACTCTTGGATTCAGCAATTCCAGTGACGTCCTCCCGTTGTACATTGGGGATGATCGGACTGATGAAGATGCTTTCAAGGTGATACGAAGTAGAGGACTAGGGTTTCCGATAATTGTGTCTTCAACCCCAAAGGATACCAAAGCTTGCTATTCCCTGCAGGATCCGTCGGAGGTGTTAACTTTCTTGTTACGGATTGCAAGGTGGAGAAAAGCGTCTTCTTCGAGCAGGTCACTTGCTCAAATTTGGGGTCCTGGGAATTCACCTAGATCAGTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Trehalose_PPase

Protein Analysis

377

Amino Acids

42.12

Weight (kDa)

9.02

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 97 - 340 3.6e-63 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 204
AatII GACGTC 1 cut(s) 882
Acc36I ACCTGC 3 cut(s) 162, 586, 1068
AccB7I CCANNNNNTGG 2 cut(s) 481, 816
AciI CCGC 2 cut(s) 373, 694
AclWI GGATC 3 cut(s) 323, 1007, 1020
AcoI YGGCCR 1 cut(s) 803
AcsI RAATTY 3 cut(s) 764, 1092, 1108
AcyI GRCGYC 1 cut(s) 879
AfaI GTAC 5 cut(s) 444, 687, 747, 801, 893
AfiI CCNNNNNNNGG 5 cut(s) 40, 481, 521, 709, 816
AgsI TTSAA 4 cut(s) 575, 752, 928, 975
AjnI CCWGG 4 cut(s) 209, 635, 702, 1102
AluBI AGCT 3 cut(s) 28, 200, 995
AluI AGCT 3 cut(s) 28, 200, 995
Alw21I GWGCWC 1 cut(s) 225
AlwI GGATC 3 cut(s) 323, 1007, 1020
AlwNI CAGNNNCTG 1 cut(s) 675
AoxI GGCC 2 cut(s) 503, 803
ApeKI GCWGC 2 cut(s) 263, 365
ApoI RAATTY 3 cut(s) 764, 1092, 1108
ArsI GACNNNNNNTTYG 4 cut(s) 725, 757, 1084, 1116
AspS9I GGNCC 4 cut(s) 306, 493, 700, 1100
AsuHPI GGTGA 7 cut(s) 16, 136, 306, 748, 766, 943, 1104
AvaII GGWCC 4 cut(s) 306, 493, 700, 1100
BamHI GGATCC 1 cut(s) 1012
BanII GRGCYC 1 cut(s) 242
BbsI GAAGAC 2 cut(s) 963, 1059
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 2 cut(s) 275, 352
BccI CCATC 6 cut(s) 95, 274, 296, 475, 515, 814
BceAI ACGGC 1 cut(s) 818
BciT130I CCWGG 4 cut(s) 211, 637, 704, 1104
BciVI GTATCC 1 cut(s) 979
BclI TGATCA 2 cut(s) 6, 247
BfaI CTAG 5 cut(s) 293, 654, 836, 950, 1116
BfmI CTRYAG 3 cut(s) 366, 537, 1007
BfuAI ACCTGC 3 cut(s) 162, 586, 1068
BfuI GTATCC 1 cut(s) 979
BisI GCNGC 2 cut(s) 264, 366
BlsI GCNGC 2 cut(s) 265, 367
Bme1390I CCNGG 4 cut(s) 211, 637, 704, 1104
Bme18I GGWCC 4 cut(s) 306, 493, 700, 1100
BmgT120I GGNCC 4 cut(s) 306, 493, 700, 1100
BmiI GGNNCC 5 cut(s) 307, 308, 495, 1014, 1101
BmrFI CCNGG 4 cut(s) 211, 637, 704, 1104
BmsI GCATC 3 cut(s) 146, 348, 910
BpiI GAAGAC 2 cut(s) 963, 1059
Bpu10I CCTNAGC 1 cut(s) 133
BsaHI GRCGYC 1 cut(s) 879
BsaJI CCNNGG 5 cut(s) 210, 475, 500, 543, 1103
Bsc4I CCNNNNNNNGG 5 cut(s) 40, 481, 521, 709, 816
Bse1I ACTGG 1 cut(s) 873
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 4 cut(s) 211, 637, 704, 1104
BseDI CCNNGG 5 cut(s) 210, 475, 500, 543, 1103
BseGI GGATG 5 cut(s) 285, 486, 712, 806, 907
BseLI CCNNNNNNNGG 5 cut(s) 40, 481, 521, 709, 816
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 1 cut(s) 873
BseRI GAGGAG 1 cut(s) 641
BseXI GCAGC 2 cut(s) 275, 352
BshFI GGCC 2 cut(s) 505, 805
BsiHKAI GWGCWC 1 cut(s) 225
BslFI GGGAC 3 cut(s) 57, 319, 479
BslI CCNNNNNNNGG 5 cut(s) 40, 481, 521, 709, 816
BsmFI GGGAC 3 cut(s) 57, 319, 479
BsnI GGCC 2 cut(s) 505, 805
Bsp1286I GDGCHC 2 cut(s) 225, 242
Bsp1407I TGTACA 3 cut(s) 442, 685, 891
Bsp143I GATC 6 cut(s) 6, 247, 328, 904, 1012, 1118
Bsp19I CCATGG 2 cut(s) 475, 543
BspACI CCGC 2 cut(s) 373, 694
BspANI GGCC 2 cut(s) 505, 805
BspCNI CTCAG 1 cut(s) 203
BspHI TCATGA 1 cut(s) 9
BspLI GGNNCC 5 cut(s) 307, 308, 495, 1014, 1101
BspMAI CTGCAG 2 cut(s) 370, 1011
BspMI ACCTGC 3 cut(s) 162, 586, 1068
BspPI GGATC 3 cut(s) 323, 1007, 1020
BsrDI GCAATG 1 cut(s) 45
BsrGI TGTACA 3 cut(s) 442, 685, 891
BsrI ACTGG 1 cut(s) 873
BssECI CCNNGG 5 cut(s) 210, 475, 500, 543, 1103
BssMI GATC 6 cut(s) 6, 247, 328, 904, 1012, 1118
BssNI GRCGYC 1 cut(s) 879
BssT1I CCWWGG 3 cut(s) 475, 500, 543
Bst2UI CCWGG 4 cut(s) 211, 637, 704, 1104
Bst4CI ACNGT 4 cut(s) 119, 338, 447, 621
Bst6I CTCTTC 1 cut(s) 269
BstACI GRCGYC 1 cut(s) 879
BstAPI GCANNNNNTGC 1 cut(s) 79
BstAUI TGTACA 3 cut(s) 442, 685, 891
BstC8I GCNNGC 3 cut(s) 363, 597, 997
BstDEI CTNAG 2 cut(s) 133, 190
BstDSI CCRYGG 2 cut(s) 475, 543
BstF5I GGATG 5 cut(s) 285, 486, 712, 806, 907
BstKTI GATC 6 cut(s) 9, 250, 331, 907, 1015, 1121
BstMBI GATC 6 cut(s) 6, 247, 328, 904, 1012, 1118
BstMWI GCNNNNNNNGC 2 cut(s) 79, 511
BstNI CCWGG 4 cut(s) 211, 637, 704, 1104
BstSCI CCNGG 4 cut(s) 209, 635, 702, 1102
BstSFI CTRYAG 3 cut(s) 366, 537, 1007
BstV1I GCAGC 2 cut(s) 275, 352
BstV2I GAAGAC 2 cut(s) 963, 1059
BstX2I RGATCY 1 cut(s) 1012
BstYI RGATCY 1 cut(s) 1012
BsuI GTATCC 1 cut(s) 979
BsuRI GGCC 2 cut(s) 505, 805
BtgI CCRYGG 2 cut(s) 475, 543
BtgZI GCGATG 1 cut(s) 534
BtsCI GGATG 5 cut(s) 285, 486, 712, 806, 907
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 4 cut(s) 124, 375, 617, 880
BveI ACCTGC 3 cut(s) 162, 586, 1068
Cac8I GCNNGC 3 cut(s) 363, 597, 997
CaiI CAGNNNCTG 1 cut(s) 675
CciI TCATGA 1 cut(s) 9
Cfr13I GGNCC 4 cut(s) 306, 493, 700, 1100
CseI GACGC 1 cut(s) 1053
Csp6I GTAC 5 cut(s) 443, 686, 746, 800, 892
CviAII CATG 6 cut(s) 10, 158, 346, 476, 544, 830
CviJI RGCY 9 cut(s) 28, 200, 240, 474, 505, 514, 542, 805, 995
CviKI_1 RGCY 9 cut(s) 28, 200, 240, 474, 505, 514, 542, 805, 995
CviQI GTAC 5 cut(s) 443, 686, 746, 800, 892
DdeI CTNAG 2 cut(s) 133, 190
DpnI GATC 6 cut(s) 8, 249, 330, 906, 1014, 1120
DpnII GATC 6 cut(s) 6, 247, 328, 904, 1012, 1118
EaeI YGGCCR 1 cut(s) 803
Eam1104I CTCTTC 1 cut(s) 269
EarI CTCTTC 1 cut(s) 269
Eco130I CCWWGG 3 cut(s) 475, 500, 543
Eco24I GRGCYC 1 cut(s) 242
Eco47I GGWCC 4 cut(s) 306, 493, 700, 1100
EcoO109I RGGNCCY 2 cut(s) 306, 1100
EcoRI GAATTC 1 cut(s) 1108
EcoRII CCWGG 4 cut(s) 209, 635, 702, 1102
EcoT14I CCWWGG 3 cut(s) 475, 500, 543
EcoT38I GRGCYC 1 cut(s) 242
ErhI CCWWGG 3 cut(s) 475, 500, 543
FaeI CATG 6 cut(s) 13, 161, 349, 479, 547, 833
FaqI GGGAC 3 cut(s) 57, 319, 479
FatI CATG 6 cut(s) 9, 157, 345, 475, 543, 829
FauI CCCGC 1 cut(s) 366
FbaI TGATCA 2 cut(s) 6, 247
Fnu4HI GCNGC 2 cut(s) 264, 366
FokI GGATG 5 cut(s) 292, 493, 719, 793, 914
FriOI GRGCYC 1 cut(s) 242
Fsp4HI GCNGC 2 cut(s) 264, 366
FspBI CTAG 5 cut(s) 293, 654, 836, 950, 1116
GluI GCNGC 2 cut(s) 264, 366
HaeIII GGCC 2 cut(s) 505, 805
HgaI GACGC 1 cut(s) 1053
Hin1I GRCGYC 1 cut(s) 879
Hin1II CATG 6 cut(s) 13, 161, 349, 479, 547, 833
HincII GTYRAC 1 cut(s) 1029
HindII GTYRAC 1 cut(s) 1029
HindIII AAGCTT 1 cut(s) 993
HinfI GANTC 3 cut(s) 45, 672, 861
HpaI GTTAAC 1 cut(s) 1029
HphI GGTGA 7 cut(s) 16, 136, 306, 748, 766, 943, 1104
Hpy166II GTNNAC 1 cut(s) 1029
Hpy188I TCNGA 6 cut(s) 193, 252, 352, 909, 960, 1021
Hpy188III TCNNGA 4 cut(s) 10, 91, 332, 761
Hpy8I GTNNAC 1 cut(s) 1029
Hpy99I CGWCG 1 cut(s) 1021
HpyAV CCTTC 5 cut(s) 237, 352, 408, 641, 787
HpyCH4III ACNGT 4 cut(s) 119, 338, 447, 621
HpyCH4IV ACGT 2 cut(s) 564, 879
HpyCH4V TGCA 7 cut(s) 82, 157, 233, 368, 599, 1009, 1049
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 511
HpyF3I CTNAG 2 cut(s) 133, 190
HpySE526I ACGT 2 cut(s) 564, 879
Hsp92I GRCGYC 1 cut(s) 879
Hsp92II CATG 6 cut(s) 13, 161, 349, 479, 547, 833
KflI GGGWCCC 1 cut(s) 306
Ksp22I TGATCA 2 cut(s) 6, 247
KspAI GTTAAC 1 cut(s) 1029
Kzo9I GATC 6 cut(s) 6, 247, 328, 904, 1012, 1118
Lsp1109I GCAGC 2 cut(s) 275, 352
LweI GCATC 3 cut(s) 146, 348, 910
MaeI CTAG 5 cut(s) 293, 654, 836, 950, 1116
MaeII ACGT 2 cut(s) 564, 879
MaeIII GTNAC 4 cut(s) 560, 875, 1038, 1080
MalI GATC 6 cut(s) 8, 249, 330, 906, 1014, 1120
MboI GATC 6 cut(s) 6, 247, 328, 904, 1012, 1118
MboII GAAGA 5 cut(s) 286, 929, 963, 1059, 1062
MfeI CAATTG 1 cut(s) 318
MflI RGATCY 1 cut(s) 1012
MhlI GDGCHC 2 cut(s) 225, 242
MlyI GAGTC 1 cut(s) 39
MmeI TCCRAC 2 cut(s) 419, 999
MseI TTAA 3 cut(s) 449, 1028, 1125
MspR9I CCNGG 4 cut(s) 211, 637, 704, 1104
MunI CAATTG 1 cut(s) 318
MvaI CCWGG 4 cut(s) 211, 637, 704, 1104
MwoI GCNNNNNNNGC 2 cut(s) 79, 511
NcoI CCATGG 2 cut(s) 475, 543
NdeII GATC 6 cut(s) 6, 247, 328, 904, 1012, 1118
NlaIII CATG 6 cut(s) 13, 161, 349, 479, 547, 833
NlaIV GGNNCC 5 cut(s) 307, 308, 495, 1014, 1101
NmuCI GTSAC 3 cut(s) 560, 875, 1080
PagI TCATGA 1 cut(s) 9
PfeI GAWTC 2 cut(s) 672, 861
PflMI CCANNNNNTGG 2 cut(s) 481, 816
PfoI TCCNGGA 1 cut(s) 702
PkrI GCNGC 2 cut(s) 265, 367
PleI GAGTC 1 cut(s) 39
PpsI GAGTC 1 cut(s) 39
PpuMI RGGWCCY 2 cut(s) 306, 1100
PsiI TTATAA 1 cut(s) 204
Psp5II RGGWCCY 2 cut(s) 306, 1100
Psp6I CCWGG 4 cut(s) 209, 635, 702, 1102
PspGI CCWGG 4 cut(s) 209, 635, 702, 1102
PspN4I GGNNCC 5 cut(s) 307, 308, 495, 1014, 1101
PspPI GGNCC 4 cut(s) 306, 493, 700, 1100
PspPPI RGGWCCY 2 cut(s) 306, 1100
PstI CTGCAG 2 cut(s) 370, 1011
PstNI CAGNNNCTG 1 cut(s) 675
PsuI RGATCY 1 cut(s) 1012
RsaI GTAC 5 cut(s) 444, 687, 747, 801, 893
RsaNI GTAC 5 cut(s) 443, 686, 746, 800, 892
SaqAI TTAA 3 cut(s) 449, 1028, 1125
SatI GCNGC 2 cut(s) 264, 366
Sau3AI GATC 6 cut(s) 6, 247, 328, 904, 1012, 1118
Sau96I GGNCC 4 cut(s) 306, 493, 700, 1100
SbfI CCTGCAGG 1 cut(s) 1011
SchI GAGTC 1 cut(s) 39
ScrFI CCNGG 4 cut(s) 211, 637, 704, 1104
SdaI CCTGCAGG 1 cut(s) 1011
SduI GDGCHC 2 cut(s) 225, 242
SfaNI GCATC 3 cut(s) 146, 348, 910
SfcI CTRYAG 3 cut(s) 366, 537, 1007
SinI GGWCC 4 cut(s) 306, 493, 700, 1100
Sse8387I CCTGCAGG 1 cut(s) 1011
SsiI CCGC 2 cut(s) 373, 694
SspI AATATT 1 cut(s) 842
SspMI CTAG 5 cut(s) 293, 654, 836, 950, 1116
StyD4I CCNGG 4 cut(s) 209, 635, 702, 1102
StyI CCWWGG 3 cut(s) 475, 500, 543
TaaI ACNGT 4 cut(s) 119, 338, 447, 621
TaiI ACGT 2 cut(s) 567, 882
TaqI TCGA 1 cut(s) 1073
TatI WGTACW 4 cut(s) 442, 685, 745, 891
TfiI GAWTC 2 cut(s) 672, 861
Tru1I TTAA 3 cut(s) 449, 1028, 1125
Tru9I TTAA 3 cut(s) 449, 1028, 1125
TscAI CASTG 4 cut(s) 124, 375, 624, 880
TseFI GTSAC 3 cut(s) 560, 875, 1080
TseI GCWGC 2 cut(s) 263, 365
Tsp45I GTSAC 3 cut(s) 560, 875, 1080
TspDTI ATGAA 4 cut(s) 272, 334, 758, 930
TspGWI ACGGA 2 cut(s) 1005, 1057
TspRI CASTG 4 cut(s) 124, 375, 624, 880
Van91I CCANNNNNTGG 2 cut(s) 481, 816
VpaK11BI GGWCC 4 cut(s) 306, 493, 700, 1100
XapI RAATTY 3 cut(s) 764, 1092, 1108
XcmI CCANNNNNNNNNTGG 1 cut(s) 813
XspI CTAG 5 cut(s) 293, 654, 836, 950, 1116
ZraI GACGTC 1 cut(s) 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.