pycom07g25940

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008155
Physical Location & Seq
Reverse (-)
26107989 .. 26109622
1634 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g25940.6

Sequence Viewer

Length: 831 bp
ATGGGACCCTTTCACCAATTTATGATCGATCCTGACCGTGCCTTCATGTCTGATGAGATGCGTGCTGCAGTACGGGAAGTTGCCAAGTATTTTCCAACAGCAGTAATTAGTGGAAGGAGTAGAGATAAGGTTAAAGGATTTGTACAGTTAAGTAATGTATATTATGCTGGAAGCCATGGGATGGATATAATGGTCCCACCAAGGCCACTAAACCCCTGCGATGGCAAGAATCACACTACAACCATGGATAAAAAGGCGAGTGACGTTCTCTTTCAACCTGCTAAAAGATTTCTGCCTGCAATCCAAGAGATACGAACACTGTTAGAGGAGATAACCAGGAAAATAGAAGGTGCTAGGGTAGAGGACAACAGATTCTGCATCTCTGTACATTTCCGCAAGGTCCGCGATGAGGATTATGACATATTAGAAGCAAAGGTAAAGTCTGTACTTGAAAACTATCCTGAATTTCATCTCACCTTCGGCAAAAAGGTCTTGGAAGTACGGCCATCCATAGAATGGAACAAAGGTCATGCCCTAGAATATTTACTTGACACTCTTGGATTCAGCAATTCCAGTGACGTCCTCCCGTTGTACATTGGGGATGATCGGTCTGATGAAGATGCTTTCAAGGTGATACGAAGTAGAGGACTAGGGTTTCCGATAATTGTGTCTTCAACCCCAAAGGATACCAAAGCTTGCTATTCCCTGCAGGATCCGTCGGAGGTGTTAACTTTCTTGTTACGGATTGCAAGGTGGAGAAAAGCGTCTTCTTCGAGCAGGTCACTTGCTCAAATTTGGGGTCCTGGGAATTTACCTAGATCAGTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Trehalose_PPase

Protein Analysis

277

Amino Acids

31.36

Weight (kDa)

8.86

Isoelectric Point (pI)

47.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 582
Acc36I ACCTGC 2 cut(s) 286, 768
AccB7I CCANNNNNTGG 2 cut(s) 181, 516
AccII CGCG 1 cut(s) 405
AciI CCGC 2 cut(s) 394, 403
AclWI GGATC 3 cut(s) 23, 707, 720
AcoI YGGCCR 1 cut(s) 503
AcsI RAATTY 3 cut(s) 464, 792, 808
AcyI GRCGYC 1 cut(s) 579
AfaI GTAC 6 cut(s) 72, 144, 387, 447, 501, 593
AfiI CCNNNNNNNGG 4 cut(s) 181, 221, 409, 516
AgsI TTSAA 4 cut(s) 275, 452, 628, 675
AjnI CCWGG 2 cut(s) 335, 802
AluBI AGCT 1 cut(s) 695
AluI AGCT 1 cut(s) 695
AlwI GGATC 3 cut(s) 23, 707, 720
AlwNI CAGNNNCTG 1 cut(s) 375
AoxI GGCC 2 cut(s) 203, 503
ApeKI GCWGC 1 cut(s) 65
ApoI RAATTY 3 cut(s) 464, 792, 808
ArsI GACNNNNNNTTYG 4 cut(s) 425, 457, 784, 816
AspS9I GGNCC 4 cut(s) 5, 193, 400, 800
AsuHPI GGTGA 3 cut(s) 5, 466, 643
AvaII GGWCC 4 cut(s) 5, 193, 400, 800
BamHI GGATCC 1 cut(s) 712
BbsI GAAGAC 2 cut(s) 663, 759
BbvI GCAGC 1 cut(s) 52
BccI CCATC 3 cut(s) 175, 215, 514
BceAI ACGGC 1 cut(s) 518
BciT130I CCWGG 2 cut(s) 337, 804
BciVI GTATCC 1 cut(s) 679
BfaI CTAG 4 cut(s) 354, 536, 650, 816
BfmI CTRYAG 2 cut(s) 66, 707
BfuAI ACCTGC 2 cut(s) 286, 768
BfuI GTATCC 1 cut(s) 679
BisI GCNGC 1 cut(s) 66
BlsI GCNGC 1 cut(s) 67
Bme1390I CCNGG 2 cut(s) 337, 804
Bme18I GGWCC 4 cut(s) 5, 193, 400, 800
BmgT120I GGNCC 4 cut(s) 5, 193, 400, 800
BmiI GGNNCC 5 cut(s) 6, 7, 195, 714, 801
BmrFI CCNGG 2 cut(s) 337, 804
BmsI GCATC 3 cut(s) 48, 387, 610
BpiI GAAGAC 2 cut(s) 663, 759
Bsa29I ATCGAT 1 cut(s) 27
BsaHI GRCGYC 1 cut(s) 579
BsaJI CCNNGG 4 cut(s) 175, 200, 243, 803
Bsc4I CCNNNNNNNGG 4 cut(s) 181, 221, 409, 516
Bse1I ACTGG 1 cut(s) 573
BseBI CCWGG 2 cut(s) 337, 804
BseCI ATCGAT 1 cut(s) 27
BseDI CCNNGG 4 cut(s) 175, 200, 243, 803
BseGI GGATG 3 cut(s) 186, 506, 607
BseLI CCNNNNNNNGG 4 cut(s) 181, 221, 409, 516
BseNI ACTGG 1 cut(s) 573
BseRI GAGGAG 1 cut(s) 341
BseXI GCAGC 1 cut(s) 52
Bsh1236I CGCG 1 cut(s) 405
BshFI GGCC 2 cut(s) 205, 505
BshVI ATCGAT 1 cut(s) 27
BslFI GGGAC 2 cut(s) 18, 179
BslI CCNNNNNNNGG 4 cut(s) 181, 221, 409, 516
BsmFI GGGAC 2 cut(s) 18, 179
BsnI GGCC 2 cut(s) 205, 505
Bsp1407I TGTACA 3 cut(s) 142, 385, 591
Bsp143I GATC 5 cut(s) 24, 28, 604, 712, 818
Bsp19I CCATGG 2 cut(s) 175, 243
BspACI CCGC 2 cut(s) 394, 403
BspANI GGCC 2 cut(s) 205, 505
BspDI ATCGAT 1 cut(s) 27
BspFNI CGCG 1 cut(s) 405
BspLI GGNNCC 5 cut(s) 6, 7, 195, 714, 801
BspMAI CTGCAG 2 cut(s) 70, 711
BspMI ACCTGC 2 cut(s) 286, 768
BspPI GGATC 3 cut(s) 23, 707, 720
BsrGI TGTACA 3 cut(s) 142, 385, 591
BsrI ACTGG 1 cut(s) 573
BssECI CCNNGG 4 cut(s) 175, 200, 243, 803
BssMI GATC 5 cut(s) 24, 28, 604, 712, 818
BssNI GRCGYC 1 cut(s) 579
BssT1I CCWWGG 3 cut(s) 175, 200, 243
Bst2UI CCWGG 2 cut(s) 337, 804
Bst4CI ACNGT 3 cut(s) 38, 147, 321
BstACI GRCGYC 1 cut(s) 579
BstAUI TGTACA 3 cut(s) 142, 385, 591
BstC8I GCNNGC 3 cut(s) 63, 297, 697
BstDSI CCRYGG 2 cut(s) 175, 243
BstF5I GGATG 3 cut(s) 186, 506, 607
BstFNI CGCG 1 cut(s) 405
BstKTI GATC 5 cut(s) 27, 31, 607, 715, 821
BstMBI GATC 5 cut(s) 24, 28, 604, 712, 818
BstMWI GCNNNNNNNGC 1 cut(s) 402
BstNI CCWGG 2 cut(s) 337, 804
BstSCI CCNGG 2 cut(s) 335, 802
BstSFI CTRYAG 2 cut(s) 66, 707
BstUI CGCG 1 cut(s) 405
BstV1I GCAGC 1 cut(s) 52
BstV2I GAAGAC 2 cut(s) 663, 759
BstX2I RGATCY 1 cut(s) 712
BstYI RGATCY 1 cut(s) 712
Bsu15I ATCGAT 1 cut(s) 27
BsuI GTATCC 1 cut(s) 679
BsuRI GGCC 2 cut(s) 205, 505
BsuTUI ATCGAT 1 cut(s) 27
BtgI CCRYGG 2 cut(s) 175, 243
BtgZI GCGATG 2 cut(s) 234, 420
BtsCI GGATG 3 cut(s) 186, 506, 607
BtsIMutI CAGTG 2 cut(s) 317, 580
BveI ACCTGC 2 cut(s) 286, 768
Cac8I GCNNGC 3 cut(s) 63, 297, 697
CaiI CAGNNNCTG 1 cut(s) 375
Cfr13I GGNCC 4 cut(s) 5, 193, 400, 800
ClaI ATCGAT 1 cut(s) 27
CseI GACGC 1 cut(s) 753
Csp6I GTAC 6 cut(s) 71, 143, 386, 446, 500, 592
CviAII CATG 4 cut(s) 46, 176, 244, 530
CviJI RGCY 4 cut(s) 174, 205, 505, 695
CviKI_1 RGCY 4 cut(s) 174, 205, 505, 695
CviQI GTAC 6 cut(s) 71, 143, 386, 446, 500, 592
DpnI GATC 5 cut(s) 26, 30, 606, 714, 820
DpnII GATC 5 cut(s) 24, 28, 604, 712, 818
EaeI YGGCCR 1 cut(s) 503
Eco130I CCWWGG 3 cut(s) 175, 200, 243
Eco47I GGWCC 4 cut(s) 5, 193, 400, 800
EcoO109I RGGNCCY 2 cut(s) 5, 800
EcoRII CCWGG 2 cut(s) 335, 802
EcoT14I CCWWGG 3 cut(s) 175, 200, 243
ErhI CCWWGG 3 cut(s) 175, 200, 243
FaeI CATG 4 cut(s) 49, 179, 247, 533
FaqI GGGAC 2 cut(s) 18, 179
FatI CATG 4 cut(s) 45, 175, 243, 529
Fnu4HI GCNGC 1 cut(s) 66
FokI GGATG 3 cut(s) 193, 493, 614
Fsp4HI GCNGC 1 cut(s) 66
FspBI CTAG 4 cut(s) 354, 536, 650, 816
GluI GCNGC 1 cut(s) 66
HaeIII GGCC 2 cut(s) 205, 505
HgaI GACGC 1 cut(s) 753
Hin1I GRCGYC 1 cut(s) 579
Hin1II CATG 4 cut(s) 49, 179, 247, 533
HincII GTYRAC 1 cut(s) 729
HindII GTYRAC 1 cut(s) 729
HindIII AAGCTT 1 cut(s) 693
HinfI GANTC 3 cut(s) 229, 372, 561
HpaI GTTAAC 1 cut(s) 729
HphI GGTGA 3 cut(s) 5, 466, 643
Hpy166II GTNNAC 1 cut(s) 729
Hpy188I TCNGA 4 cut(s) 52, 613, 660, 721
Hpy188III TCNNGA 2 cut(s) 32, 461
Hpy8I GTNNAC 1 cut(s) 729
Hpy99I CGWCG 1 cut(s) 721
HpyAV CCTTC 4 cut(s) 52, 108, 341, 487
HpyCH4III ACNGT 3 cut(s) 38, 147, 321
HpyCH4IV ACGT 2 cut(s) 264, 579
HpyCH4V TGCA 5 cut(s) 68, 299, 378, 709, 749
HpyF10VI GCNNNNNNNGC 1 cut(s) 402
HpySE526I ACGT 2 cut(s) 264, 579
Hsp92I GRCGYC 1 cut(s) 579
Hsp92II CATG 4 cut(s) 49, 179, 247, 533
KflI GGGWCCC 1 cut(s) 5
KspAI GTTAAC 1 cut(s) 729
Kzo9I GATC 5 cut(s) 24, 28, 604, 712, 818
Lsp1109I GCAGC 1 cut(s) 52
LweI GCATC 3 cut(s) 48, 387, 610
MaeI CTAG 4 cut(s) 354, 536, 650, 816
MaeII ACGT 2 cut(s) 264, 579
MaeIII GTNAC 4 cut(s) 260, 575, 738, 780
MalI GATC 5 cut(s) 26, 30, 606, 714, 820
MboI GATC 5 cut(s) 24, 28, 604, 712, 818
MboII GAAGA 4 cut(s) 629, 663, 759, 762
MflI RGATCY 1 cut(s) 712
MluCI AATT 8 cut(s) 17, 105, 464, 568, 663, 792, 808, 826
MmeI TCCRAC 2 cut(s) 119, 699
MnlI CCTC 6 cut(s) 319, 355, 403, 593, 638, 715
MseI TTAA 4 cut(s) 132, 149, 728, 825
MspR9I CCNGG 2 cut(s) 337, 804
MvaI CCWGG 2 cut(s) 337, 804
MvnI CGCG 1 cut(s) 405
MwoI GCNNNNNNNGC 1 cut(s) 402
NcoI CCATGG 2 cut(s) 175, 243
NdeII GATC 5 cut(s) 24, 28, 604, 712, 818
NlaIII CATG 4 cut(s) 49, 179, 247, 533
NlaIV GGNNCC 5 cut(s) 6, 7, 195, 714, 801
NmuCI GTSAC 3 cut(s) 260, 575, 780
PfeI GAWTC 3 cut(s) 229, 372, 561
PflMI CCANNNNNTGG 2 cut(s) 181, 516
PkrI GCNGC 1 cut(s) 67
PpuMI RGGWCCY 2 cut(s) 5, 800
Psp5II RGGWCCY 2 cut(s) 5, 800
Psp6I CCWGG 2 cut(s) 335, 802
PspGI CCWGG 2 cut(s) 335, 802
PspN4I GGNNCC 5 cut(s) 6, 7, 195, 714, 801
PspPI GGNCC 4 cut(s) 5, 193, 400, 800
PspPPI RGGWCCY 2 cut(s) 5, 800
PstI CTGCAG 2 cut(s) 70, 711
PstNI CAGNNNCTG 1 cut(s) 375
PsuI RGATCY 1 cut(s) 712
RsaI GTAC 6 cut(s) 72, 144, 387, 447, 501, 593
RsaNI GTAC 6 cut(s) 71, 143, 386, 446, 500, 592
SaqAI TTAA 4 cut(s) 132, 149, 728, 825
SatI GCNGC 1 cut(s) 66
Sau3AI GATC 5 cut(s) 24, 28, 604, 712, 818
Sau96I GGNCC 4 cut(s) 5, 193, 400, 800
SbfI CCTGCAGG 1 cut(s) 711
ScrFI CCNGG 2 cut(s) 337, 804
SdaI CCTGCAGG 1 cut(s) 711
SfaNI GCATC 3 cut(s) 48, 387, 610
SfcI CTRYAG 2 cut(s) 66, 707
SinI GGWCC 4 cut(s) 5, 193, 400, 800
Sse8387I CCTGCAGG 1 cut(s) 711
Sse9I AATT 8 cut(s) 17, 105, 464, 568, 663, 792, 808, 826
SsiI CCGC 2 cut(s) 394, 403
SspI AATATT 1 cut(s) 542
SspMI CTAG 4 cut(s) 354, 536, 650, 816
StyD4I CCNGG 2 cut(s) 335, 802
StyI CCWWGG 3 cut(s) 175, 200, 243
TaaI ACNGT 3 cut(s) 38, 147, 321
TaiI ACGT 2 cut(s) 267, 582
TaqI TCGA 2 cut(s) 27, 773
TaqII GACCGA 1 cut(s) 597
TasI AATT 8 cut(s) 17, 105, 464, 568, 663, 792, 808, 826
TatI WGTACW 4 cut(s) 142, 385, 445, 591
TfiI GAWTC 3 cut(s) 229, 372, 561
Tru1I TTAA 4 cut(s) 132, 149, 728, 825
Tru9I TTAA 4 cut(s) 132, 149, 728, 825
TscAI CASTG 2 cut(s) 324, 580
TseFI GTSAC 3 cut(s) 260, 575, 780
TseI GCWGC 1 cut(s) 65
Tsp45I GTSAC 3 cut(s) 260, 575, 780
TspDTI ATGAA 3 cut(s) 34, 458, 630
TspGWI ACGGA 2 cut(s) 705, 757
TspRI CASTG 2 cut(s) 324, 580
Van91I CCANNNNNTGG 2 cut(s) 181, 516
VpaK11BI GGWCC 4 cut(s) 5, 193, 400, 800
XapI RAATTY 3 cut(s) 464, 792, 808
XcmI CCANNNNNNNNNTGG 1 cut(s) 513
XspI CTAG 4 cut(s) 354, 536, 650, 816
ZraI GACGTC 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.