RLG00000026320

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0008155
Physical Location & Seq
Forward (+)
2153478 .. 2160214
6737 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026320

Sequence Viewer

Length: 1203 bp
ATGCTCCTCTGGCTTTCTAGGGCAACCCAGATTTCGTCACCTTTCATCAGTCGATCGACGACCATGACAAACTTTGCTAAGCTGAACCAGGCAATGGGGTTTCAGAGATCATCAACATCTGATAAGCAAAAGGTGCAACCTATCAGTACAAAAGTAGTAACAAACGATGAGAACAGTGGTGATCTTTCCTTGGCATCAATAGCCTCAAACCTGCATGATATTTCTACCATTGTTAATACACCTATCCCCTCATCAGATACAGCTTATAGTTCATGGGTGGTGGAGCACCCTTCTGCACTAGGCTCATTTGATAGGATGATGAAAGCTGCAAAAGGGAAGAGGATTATTGTCTTTTTAGACTATGATGGCACCCTTTCACCGATTGTTGATGATCCGGATCGTGCTTTCATGTCCAATGAGATGCGTGCAGCAGTACGCGAAGTTGCCAAATATTTTCCAACAGCAGTAATCAGTGGGAGGAGTAGAGACAAGGTAAAAGGATTTGTACAGTTAAGTAATGTCTATTATGCCGGCAGCCATGGGATGGACATTATGGTCCCTCCAAAGCCACTAAAGCCCTGTGATGCCAAGAACCACGTCACAGCCACAGGCTCAGCCTTGGATAAGGAGGGTGACATGCTCTTTCAACCTGCCAAAAGATTCCTGCCTGCAATCCAAGAGATAAGCATAGTGTTGGAGGAAATAGCAAGAAAGATAGAAGGTGCAAGAGTAGAGGACAATAGATTCTGCATCTCTGTGCATTACCGGAAGGTCCGGGAAGAGGATTATGGCATATTAGAAGAAAAGGTGAAGTCTATGATTGAAAACTATCCAGATTTTCACTTAACTCGGGGTAAAAAGGTCCTGGAGATACGACCATCTATAGAATGGAACAAAGGTCATGCGCTTGAATATTTACTTGACACTCTTGGGTTCAGTAATTCGGGTGATGTATTCCCTTTGTACATCGGGGATGATCGAACGGATGAAGATGCTTTCAAGGTGATAGAAGCCAGAGGACAGGGATTTCCAATAATCGTGTCTTCCATCCCAAAAGACACCAAAGCTGCTTTTTCTCTGCACGATCCGTCAGAAGTGTTGACTTTCTTGTTACGCCTTGCAAGGTGGAGAAAAGCCTCGTCTTCAAGCAGGTCACTCGCTCAAATATGGGGTCTCGGGAATTCACATAAGGCCAATTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Trehalose_PPase

Protein Analysis

401

Amino Acids

44.56

Weight (kDa)

8.55

Isoelectric Point (pI)

37.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 118 - 364 2.5e-62 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 554
Acc36I ACCTGC 3 cut(s) 219, 658, 1140
AccB1I GGYRCC 1 cut(s) 368
AccB7I CCANNNNNTGG 2 cut(s) 94, 544
AccII CGCG 1 cut(s) 438
AccIII TCCGGA 1 cut(s) 394
AclWI GGATC 3 cut(s) 386, 405, 1079
AcsI RAATTY 1 cut(s) 1180
AfaI GTAC 4 cut(s) 148, 435, 507, 965
AfiI CCNNNNNNNGG 3 cut(s) 94, 544, 781
AgsI TTSAA 5 cut(s) 647, 824, 911, 1000, 1146
AjiI CACGTC 1 cut(s) 598
AjnI CCWGG 2 cut(s) 87, 864
AluBI AGCT 4 cut(s) 82, 263, 326, 1067
AluI AGCT 4 cut(s) 82, 263, 326, 1067
Alw21I GWGCWC 1 cut(s) 288
Alw26I GTCTC 2 cut(s) 480, 1178
AlwI GGATC 3 cut(s) 386, 405, 1079
Ama87I CYCGRG 2 cut(s) 849, 1175
Aor13HI TCCGGA 1 cut(s) 394
AoxI GGCC 1 cut(s) 1191
ApeKI GCWGC 4 cut(s) 326, 428, 534, 1067
ApoI RAATTY 1 cut(s) 1180
ArsI GACNNNNNNTTYG 2 cut(s) 1156, 1188
AspLEI GCGC 1 cut(s) 907
AspS9I GGNCC 3 cut(s) 556, 772, 862
AsuC2I CCSGG 1 cut(s) 776
AsuHPI GGTGA 7 cut(s) 30, 191, 369, 644, 820, 959, 1015
AvaI CYCGRG 2 cut(s) 849, 1175
AvaII GGWCC 3 cut(s) 556, 772, 862
BanI GGYRCC 1 cut(s) 368
BbsI GAAGAC 2 cut(s) 1035, 1134
Bbv12I GWGCWC 1 cut(s) 288
BbvI GCAGC 4 cut(s) 313, 440, 546, 1054
BccI CCATC 4 cut(s) 359, 538, 886, 1055
BcgI CGANNNNNNTGC 2 cut(s) 1138, 1172
BciT130I CCWGG 2 cut(s) 89, 866
BcnI CCSGG 1 cut(s) 776
BcoDI GTCTC 2 cut(s) 480, 1178
BfaI CTAG 3 cut(s) 18, 299, 1201
BfmI CTRYAG 1 cut(s) 882
BfuAI ACCTGC 3 cut(s) 219, 658, 1140
BisI GCNGC 4 cut(s) 327, 429, 535, 1068
BlpI GCTNAGC 2 cut(s) 78, 613
BlsI GCNGC 4 cut(s) 328, 430, 536, 1069
Bme1390I CCNGG 3 cut(s) 89, 776, 866
Bme18I GGWCC 3 cut(s) 556, 772, 862
BmeT110I CYCGRG 2 cut(s) 849, 1175
BmgBI CACGTC 1 cut(s) 598
BmgT120I GGNCC 3 cut(s) 556, 772, 862
BmiI GGNNCC 2 cut(s) 370, 558
BmrFI CCNGG 3 cut(s) 89, 776, 866
BmsI GCATC 5 cut(s) 203, 411, 574, 759, 982
BpiI GAAGAC 2 cut(s) 1035, 1134
BpmI CTGGAG 1 cut(s) 887
Bpu1102I GCTNAGC 2 cut(s) 78, 613
BpuMI CCSGG 1 cut(s) 776
BsaBI GATNNNNATC 1 cut(s) 396
BsaI GGTCTC 1 cut(s) 1178
BsaJI CCNNGG 3 cut(s) 189, 538, 618
BsaWI WCCGGW 2 cut(s) 394, 765
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 544, 781
Bse118I RCCGGY 1 cut(s) 530
Bse3DI GCAATG 1 cut(s) 99
Bse8I GATNNNNATC 1 cut(s) 396
BseAI TCCGGA 1 cut(s) 394
BseBI CCWGG 2 cut(s) 89, 866
BseDI CCNNGG 3 cut(s) 189, 538, 618
BseGI GGATG 5 cut(s) 321, 549, 979, 991, 1047
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 3 cut(s) 94, 544, 781
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 1 cut(s) 627
BseRI GAGGAG 1 cut(s) 493
BseXI GCAGC 4 cut(s) 313, 440, 546, 1054
BsgI GTGCAG 3 cut(s) 279, 447, 1064
Bsh1236I CGCG 1 cut(s) 438
Bsh1285I CGRYCG 1 cut(s) 56
BshFI GGCC 1 cut(s) 1193
BshNI GGYRCC 1 cut(s) 368
BsiEI CGRYCG 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 288
BsiHKCI CYCGRG 2 cut(s) 849, 1175
BsiSI CCGG 4 cut(s) 395, 531, 766, 775
BslFI GGGAC 1 cut(s) 542
BslI CCNNNNNNNGG 3 cut(s) 94, 544, 781
BsmAI GTCTC 2 cut(s) 480, 1178
BsmFI GGGAC 1 cut(s) 542
BsnI GGCC 1 cut(s) 1193
Bso31I GGTCTC 1 cut(s) 1178
BsoBI CYCGRG 2 cut(s) 849, 1175
Bsp1286I GDGCHC 1 cut(s) 288
Bsp13I TCCGGA 1 cut(s) 394
Bsp1407I TGTACA 2 cut(s) 505, 963
Bsp143I GATC 7 cut(s) 53, 107, 181, 391, 397, 976, 1084
Bsp1720I GCTNAGC 2 cut(s) 78, 613
Bsp19I CCATGG 1 cut(s) 538
BspANI GGCC 1 cut(s) 1193
BspCNI CTCAG 1 cut(s) 626
BspEI TCCGGA 1 cut(s) 394
BspFNI CGCG 1 cut(s) 438
BspLI GGNNCC 2 cut(s) 370, 558
BspMI ACCTGC 3 cut(s) 219, 658, 1140
BspPI GGATC 3 cut(s) 386, 405, 1079
BspT107I GGYRCC 1 cut(s) 368
BspTNI GGTCTC 1 cut(s) 1178
BsrDI GCAATG 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 530
BsrGI TGTACA 2 cut(s) 505, 963
BssAI RCCGGY 1 cut(s) 530
BssECI CCNNGG 3 cut(s) 189, 538, 618
BssMI GATC 7 cut(s) 53, 107, 181, 391, 397, 976, 1084
BssT1I CCWWGG 3 cut(s) 189, 538, 618
Bst2UI CCWGG 2 cut(s) 89, 866
Bst4CI ACNGT 2 cut(s) 176, 510
Bst6I CTCTTC 2 cut(s) 332, 774
BstAPI GCANNNNNTGC 1 cut(s) 133
BstAUI TGTACA 2 cut(s) 505, 963
BstC8I GCNNGC 3 cut(s) 426, 532, 669
BstDEI CTNAG 2 cut(s) 78, 613
BstDSI CCRYGG 1 cut(s) 538
BstF5I GGATG 5 cut(s) 321, 549, 979, 991, 1047
BstFNI CGCG 1 cut(s) 438
BstHHI GCGC 1 cut(s) 907
BstKTI GATC 7 cut(s) 56, 110, 184, 394, 400, 979, 1087
BstMAI GTCTC 2 cut(s) 480, 1178
BstMBI GATC 7 cut(s) 53, 107, 181, 391, 397, 976, 1084
BstMCI CGRYCG 1 cut(s) 56
BstMWI GCNNNNNNNGC 4 cut(s) 10, 133, 200, 574
BstNI CCWGG 2 cut(s) 89, 866
BstNSI RCATGY 1 cut(s) 640
BstSCI CCNGG 3 cut(s) 87, 774, 864
BstSFI CTRYAG 1 cut(s) 882
BstUI CGCG 1 cut(s) 438
BstV1I GCAGC 4 cut(s) 313, 440, 546, 1054
BstV2I GAAGAC 2 cut(s) 1035, 1134
BsuRI GGCC 1 cut(s) 1193
BtgI CCRYGG 1 cut(s) 538
BtrI CACGTC 1 cut(s) 598
BtsCI GGATG 5 cut(s) 321, 549, 979, 991, 1047
BtsIMutI CAGTG 2 cut(s) 181, 478
BveI ACCTGC 3 cut(s) 219, 658, 1140
Cac8I GCNNGC 3 cut(s) 426, 532, 669
CfoI GCGC 1 cut(s) 907
Cfr10I RCCGGY 1 cut(s) 530
Cfr13I GGNCC 3 cut(s) 556, 772, 862
Csp6I GTAC 4 cut(s) 147, 434, 506, 964
CviAII CATG 7 cut(s) 64, 215, 273, 409, 539, 637, 902
CviQI GTAC 4 cut(s) 147, 434, 506, 964
DdeI CTNAG 2 cut(s) 78, 613
DpnI GATC 7 cut(s) 55, 109, 183, 393, 399, 978, 1086
DpnII GATC 7 cut(s) 53, 107, 181, 391, 397, 976, 1084
DrdI GACNNNNNNGTC 1 cut(s) 554
DseDI GACNNNNNNGTC 1 cut(s) 554
Eam1104I CTCTTC 2 cut(s) 332, 774
EarI CTCTTC 2 cut(s) 332, 774
Eco130I CCWWGG 3 cut(s) 189, 538, 618
Eco31I GGTCTC 1 cut(s) 1178
Eco47I GGWCC 3 cut(s) 556, 772, 862
Eco88I CYCGRG 2 cut(s) 849, 1175
EcoO109I RGGNCCY 1 cut(s) 862
EcoRI GAATTC 1 cut(s) 1180
EcoRII CCWGG 2 cut(s) 87, 864
EcoT14I CCWWGG 3 cut(s) 189, 538, 618
ErhI CCWWGG 3 cut(s) 189, 538, 618
FaeI CATG 7 cut(s) 67, 218, 276, 412, 542, 640, 905
FaqI GGGAC 1 cut(s) 542
FatI CATG 7 cut(s) 63, 214, 272, 408, 538, 636, 901
Fnu4HI GCNGC 4 cut(s) 327, 429, 535, 1068
FokI GGATG 5 cut(s) 328, 556, 986, 998, 1034
Fsp4HI GCNGC 4 cut(s) 327, 429, 535, 1068
FspBI CTAG 3 cut(s) 18, 299, 1201
GlaI GCGC 1 cut(s) 906
GluI GCNGC 4 cut(s) 327, 429, 535, 1068
GsuI CTGGAG 1 cut(s) 887
HaeIII GGCC 1 cut(s) 1193
HapII CCGG 4 cut(s) 395, 531, 766, 775
HhaI GCGC 1 cut(s) 907
Hin1II CATG 7 cut(s) 67, 218, 276, 412, 542, 640, 905
Hin6I GCGC 1 cut(s) 905
HinP1I GCGC 1 cut(s) 905
HincII GTYRAC 1 cut(s) 1101
HindII GTYRAC 1 cut(s) 1101
HinfI GANTC 2 cut(s) 660, 744
HpaII CCGG 4 cut(s) 395, 531, 766, 775
HphI GGTGA 7 cut(s) 30, 191, 369, 644, 820, 959, 1015
Hpy166II GTNNAC 1 cut(s) 1101
Hpy188I TCNGA 4 cut(s) 105, 121, 256, 1093
Hpy188III TCNNGA 3 cut(s) 395, 833, 1177
Hpy8I GTNNAC 1 cut(s) 1101
Hpy99I CGWCG 1 cut(s) 61
HpyAV CCTTC 3 cut(s) 300, 713, 763
HpyCH4III ACNGT 2 cut(s) 176, 510
HpyCH4IV ACGT 1 cut(s) 597
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 133, 200, 574
HpyF3I CTNAG 2 cut(s) 78, 613
HpySE526I ACGT 1 cut(s) 597
Hsp92II CATG 7 cut(s) 67, 218, 276, 412, 542, 640, 905
HspAI GCGC 1 cut(s) 905
Kpn2I TCCGGA 1 cut(s) 394
KroI GCCGGC 1 cut(s) 530
KroNI GCCGGC 1 cut(s) 532
Kzo9I GATC 7 cut(s) 53, 107, 181, 391, 397, 976, 1084
LmnI GCTCC 2 cut(s) 9, 283
Lsp1109I GCAGC 4 cut(s) 313, 440, 546, 1054
LweI GCATC 5 cut(s) 203, 411, 574, 759, 982
MaeI CTAG 3 cut(s) 18, 299, 1201
MaeII ACGT 1 cut(s) 597
MaeIII GTNAC 6 cut(s) 36, 157, 598, 632, 1110, 1152
MalI GATC 7 cut(s) 55, 109, 183, 393, 399, 978, 1086
MboI GATC 7 cut(s) 53, 107, 181, 391, 397, 976, 1084
MboII GAAGA 6 cut(s) 349, 791, 812, 1001, 1035, 1134
MfeI CAATTG 1 cut(s) 1195
MhlI GDGCHC 1 cut(s) 288
MluCI AATT 3 cut(s) 940, 1180, 1195
MmeI TCCRAC 2 cut(s) 482, 675
MroI TCCGGA 1 cut(s) 394
MroNI GCCGGC 1 cut(s) 530
MseI TTAA 3 cut(s) 234, 512, 845
MslI CAYNNNNRTG 1 cut(s) 755
MspI CCGG 4 cut(s) 395, 531, 766, 775
MspR9I CCNGG 3 cut(s) 89, 776, 866
MunI CAATTG 1 cut(s) 1195
MvaI CCWGG 2 cut(s) 89, 866
MvnI CGCG 1 cut(s) 438
MwoI GCNNNNNNNGC 4 cut(s) 10, 133, 200, 574
NaeI GCCGGC 1 cut(s) 532
NciI CCSGG 1 cut(s) 776
NcoI CCATGG 1 cut(s) 538
NdeII GATC 7 cut(s) 53, 107, 181, 391, 397, 976, 1084
NgoMIV GCCGGC 1 cut(s) 530
NlaIII CATG 7 cut(s) 67, 218, 276, 412, 542, 640, 905
NlaIV GGNNCC 2 cut(s) 370, 558
NmuCI GTSAC 4 cut(s) 36, 598, 632, 1152
NspI RCATGY 1 cut(s) 640
PdiI GCCGGC 1 cut(s) 532
PfeI GAWTC 2 cut(s) 660, 744
PflMI CCANNNNNTGG 2 cut(s) 94, 544
PfoI TCCNGGA 2 cut(s) 774, 864
PkrI GCNGC 4 cut(s) 328, 430, 536, 1069
Ple19I CGATCG 1 cut(s) 56
PpuMI RGGWCCY 1 cut(s) 862
Psp5II RGGWCCY 1 cut(s) 862
Psp6I CCWGG 2 cut(s) 87, 864
PspGI CCWGG 2 cut(s) 87, 864
PspN4I GGNNCC 2 cut(s) 370, 558
PspPI GGNCC 3 cut(s) 556, 772, 862
PspPPI RGGWCCY 1 cut(s) 862
PvuI CGATCG 1 cut(s) 56
RsaI GTAC 4 cut(s) 148, 435, 507, 965
RsaNI GTAC 4 cut(s) 147, 434, 506, 964
RseI CAYNNNNRTG 1 cut(s) 755
SaqAI TTAA 3 cut(s) 234, 512, 845
SatI GCNGC 4 cut(s) 327, 429, 535, 1068
Sau3AI GATC 7 cut(s) 53, 107, 181, 391, 397, 976, 1084
Sau96I GGNCC 3 cut(s) 556, 772, 862
ScrFI CCNGG 3 cut(s) 89, 776, 866
SduI GDGCHC 1 cut(s) 288
SfaNI GCATC 5 cut(s) 203, 411, 574, 759, 982
SfcI CTRYAG 1 cut(s) 882
SinI GGWCC 3 cut(s) 556, 772, 862
SmiMI CAYNNNNRTG 1 cut(s) 755
Sse9I AATT 3 cut(s) 940, 1180, 1195
SspI AATATT 2 cut(s) 452, 914
SspMI CTAG 3 cut(s) 18, 299, 1201
StyD4I CCNGG 3 cut(s) 87, 774, 864
StyI CCWWGG 3 cut(s) 189, 538, 618
TaaI ACNGT 2 cut(s) 176, 510
TaiI ACGT 1 cut(s) 600
TaqI TCGA 3 cut(s) 52, 56, 979
TasI AATT 3 cut(s) 940, 1180, 1195
TatI WGTACW 3 cut(s) 146, 505, 963
TfiI GAWTC 2 cut(s) 660, 744
Tru1I TTAA 3 cut(s) 234, 512, 845
Tru9I TTAA 3 cut(s) 234, 512, 845
TscAI CASTG 2 cut(s) 181, 478
TseFI GTSAC 4 cut(s) 36, 598, 632, 1152
TseI GCWGC 4 cut(s) 326, 428, 534, 1067
Tsp45I GTSAC 4 cut(s) 36, 598, 632, 1152
TspDTI ATGAA 5 cut(s) 34, 261, 335, 397, 1002
TspGWI ACGGA 2 cut(s) 998, 1077
TspRI CASTG 2 cut(s) 181, 478
Van91I CCANNNNNTGG 2 cut(s) 94, 544
VpaK11BI GGWCC 3 cut(s) 556, 772, 862
XapI RAATTY 1 cut(s) 1180
XceI RCATGY 1 cut(s) 640
XcmI CCANNNNNNNNNTGG 1 cut(s) 885
XspI CTAG 3 cut(s) 18, 299, 1201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.