MD00G1004200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0014009
Physical Location & Seq
Forward (+)
661757 .. 665431
3675 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1004200.v1.1.491

Sequence Viewer

Length: 741 bp
ATGGCGTCGAATTCGAATCCGTCGCTGCAGGCTGAGATCGGACCCGACGGCCTTGCTCGCGAGGCTTCTGTCATTACCTACACGGAGAAGATAATCGAGAACGAACAGCTTCAATTAAGAAAATATATTGAAGAAAACTATTCTAAAATACGGGATGTCGAACGAGAGCTGGCACAGTTGTCAATGGAAATGAAACTCACAGCTGGGCCAAAAAAATCAGCTCTTGAACATTTGAGGAAGAAAATAGAGATGTCAACGGAGAAAATTCATGCTGCCAAGCTGAAGGAAGAACAAGCACGAAAGGTGTGGGAAGCAGCATTACAGGCTGTAAAGGACGAGGAAGCTATCAAGCAGCAGCTTTGTGAAGACTTAAACCAACTGGTGCAAGAAAGCAGCAGTTCTCAGTTTACGAGACTTGAGGAGTTAAAAAGACGACTTGAAGCTATGAACCCAAGCAGATCATCAGCTTCTGTTTCTCATGATGGGAAATCAATGGGTCCAGATGCTCCTACAGTTCCGGGATCAACAGAATCAGGTGGAGTCACTCAAAACATCCCTAATCCAAGAAATCCTGGAAATGTTTCGGTCACGAATGGGCACAATCAACAGGCACCTGTTGAGGGAGAAGGAAGAGGAAAGAAGAAAAGTCAGTTCCACGGAAGAGGGAAGGGACTTGGAGCTGTGCCCAAGGGTAGGGGACCTCCTCCGCCCGGCTGGACAGGGGCTGGGTTCGATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transcrip_act

Protein Analysis

247

Amino Acids

26.82

Weight (kDa)

8.53

Isoelectric Point (pI)

48.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transcrip_act PF04949 23 - 147 1.3e-09 Transcriptional activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014009)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51840
fragaria_vesca FvH4_7g28720 FvH4_7g28720
malus_domestica MD00G1004200.v1.1 MD01G1193100.v1.1
prunus_persica Prupe.2G287200_v2.0.a1
pyrus_communis pycom01g20370
rosa_chinensis RchiOBHm_Chr1g0376221
rosa_laevigata RLG00000026618
rosa_roxburghii Rroxscaffold_4G00282290
rosa_rugosa Rorug01G0394500 Rorug01G0394600
rosa_samantha Rh1AG406100 Rh1BG369800 Rh1CG382700 Rh1DG399600
rosa_wichuraiana Rw1G036030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 610
AccII CGCG 1 cut(s) 60
AciI CCGC 1 cut(s) 707
AclWI GGATC 1 cut(s) 529
AcsI RAATTY 2 cut(s) 10, 264
AcuI CTGAAG 1 cut(s) 302
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 3 cut(s) 620, 693, 710
AgsI TTSAA 4 cut(s) 113, 131, 227, 440
AjnI CCWGG 1 cut(s) 571
Alw26I GTCTC 1 cut(s) 406
AlwI GGATC 1 cut(s) 529
AlwNI CAGNNNCTG 2 cut(s) 470, 725
AoxI GGCC 2 cut(s) 49, 206
ApeKI GCWGC 6 cut(s) 25, 272, 314, 352, 355, 393
ApoI RAATTY 2 cut(s) 10, 264
Asp700I GAANNNNTTC 2 cut(s) 108, 580
AspS9I GGNCC 4 cut(s) 41, 206, 497, 698
AsuC2I CCSGG 2 cut(s) 519, 711
AsuII TTCGAA 1 cut(s) 14
AvaII GGWCC 3 cut(s) 41, 497, 698
BaeGI GKGCMC 2 cut(s) 600, 687
BanI GGYRCC 1 cut(s) 610
BarI GAAGNNNNNNTAC 2 cut(s) 303, 335
BbsI GAAGAC 1 cut(s) 372
BbvI GCAGC 6 cut(s) 12, 259, 326, 364, 367, 405
BccI CCATC 1 cut(s) 476
BceAI ACGGC 1 cut(s) 64
BcgI CGANNNNNNTGC 2 cut(s) 35, 69
BciT130I CCWGG 1 cut(s) 573
BcnI CCSGG 2 cut(s) 519, 711
BcoDI GTCTC 1 cut(s) 406
BfmI CTRYAG 2 cut(s) 26, 510
BisI GCNGC 6 cut(s) 26, 273, 315, 353, 356, 394
BlsI GCNGC 6 cut(s) 27, 274, 316, 354, 357, 395
Bme1390I CCNGG 3 cut(s) 519, 573, 711
Bme18I GGWCC 3 cut(s) 41, 497, 698
BmgT120I GGNCC 4 cut(s) 41, 206, 497, 698
BmiI GGNNCC 4 cut(s) 43, 498, 612, 699
BmrFI CCNGG 3 cut(s) 519, 573, 711
BmsI GCATC 2 cut(s) 493, 724
BpiI GAAGAC 1 cut(s) 372
Bpu14I TTCGAA 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 437
BpuMI CCSGG 2 cut(s) 519, 711
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 655, 687
Bsc4I CCNNNNNNNGG 3 cut(s) 620, 693, 710
Bse1I ACTGG 1 cut(s) 384
BseBI CCWGG 1 cut(s) 573
BseDI CCNNGG 2 cut(s) 655, 687
BseGI GGATG 2 cut(s) 160, 552
BseLI CCNNNNNNNGG 3 cut(s) 620, 693, 710
BseMII CTCAG 2 cut(s) 24, 416
BseNI ACTGG 1 cut(s) 384
BseRI GAGGAG 2 cut(s) 434, 693
BseSI GKGCMC 2 cut(s) 600, 687
BseXI GCAGC 6 cut(s) 12, 259, 326, 364, 367, 405
BseYI CCCAGC 2 cut(s) 203, 725
Bsh1236I CGCG 1 cut(s) 60
BshFI GGCC 2 cut(s) 51, 208
BshNI GGYRCC 1 cut(s) 610
BsiSI CCGG 2 cut(s) 518, 711
BslFI GGGAC 2 cut(s) 684, 711
BslI CCNNNNNNNGG 3 cut(s) 620, 693, 710
BsmAI GTCTC 1 cut(s) 406
BsmFI GGGAC 2 cut(s) 684, 711
BsnI GGCC 2 cut(s) 51, 208
Bsp119I TTCGAA 1 cut(s) 14
Bsp1286I GDGCHC 2 cut(s) 600, 687
Bsp143I GATC 3 cut(s) 36, 458, 521
Bsp68I TCGCGA 1 cut(s) 60
BspACI CCGC 1 cut(s) 707
BspANI GGCC 2 cut(s) 51, 208
BspCNI CTCAG 2 cut(s) 25, 415
BspFNI CGCG 1 cut(s) 60
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 4 cut(s) 43, 498, 612, 699
BspMAI CTGCAG 1 cut(s) 30
BspPI GGATC 1 cut(s) 529
BspT104I TTCGAA 1 cut(s) 14
BspT107I GGYRCC 1 cut(s) 610
BsrI ACTGG 1 cut(s) 384
BssECI CCNNGG 2 cut(s) 655, 687
BssMI GATC 3 cut(s) 36, 458, 521
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 687
Bst2UI CCWGG 1 cut(s) 573
Bst4CI ACNGT 2 cut(s) 177, 514
Bst6I CTCTTC 2 cut(s) 625, 655
BstACI GRCGYC 1 cut(s) 5
BstBI TTCGAA 1 cut(s) 14
BstC8I GCNNGC 3 cut(s) 30, 58, 171
BstDEI CTNAG 3 cut(s) 33, 402, 738
BstDSI CCRYGG 1 cut(s) 655
BstF5I GGATG 2 cut(s) 160, 552
BstFNI CGCG 1 cut(s) 60
BstKTI GATC 3 cut(s) 39, 461, 524
BstMAI GTCTC 1 cut(s) 406
BstMBI GATC 3 cut(s) 36, 458, 521
BstMWI GCNNNNNNNGC 3 cut(s) 57, 62, 323
BstNI CCWGG 1 cut(s) 573
BstSCI CCNGG 3 cut(s) 517, 571, 709
BstSFI CTRYAG 2 cut(s) 26, 510
BstSLI GKGCMC 2 cut(s) 600, 687
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 6 cut(s) 12, 259, 326, 364, 367, 405
BstV2I GAAGAC 1 cut(s) 372
BsuRI GGCC 2 cut(s) 51, 208
BtgI CCRYGG 1 cut(s) 655
BtsCI GGATG 2 cut(s) 160, 552
BtuMI TCGCGA 1 cut(s) 60
Cac8I GCNNGC 3 cut(s) 30, 58, 171
CaiI CAGNNNCTG 2 cut(s) 470, 725
CciI TCATGA 1 cut(s) 478
Cfr13I GGNCC 4 cut(s) 41, 206, 497, 698
CviAII CATG 2 cut(s) 269, 479
DdeI CTNAG 3 cut(s) 33, 402, 738
DpnI GATC 3 cut(s) 38, 460, 523
DpnII GATC 3 cut(s) 36, 458, 521
Eam1104I CTCTTC 2 cut(s) 625, 655
EarI CTCTTC 2 cut(s) 625, 655
EciI GGCGGA 1 cut(s) 696
Eco130I CCWWGG 1 cut(s) 687
Eco47I GGWCC 3 cut(s) 41, 497, 698
Eco57I CTGAAG 1 cut(s) 302
EcoO109I RGGNCCY 1 cut(s) 698
EcoRI GAATTC 1 cut(s) 10
EcoRII CCWGG 1 cut(s) 571
EcoT14I CCWWGG 1 cut(s) 687
ErhI CCWWGG 1 cut(s) 687
FaeI CATG 2 cut(s) 272, 482
FaiI YATR 4 cut(s) 126, 270, 446, 480
FaqI GGGAC 2 cut(s) 684, 711
FatI CATG 2 cut(s) 268, 478
Fnu4HI GCNGC 6 cut(s) 26, 273, 315, 353, 356, 394
FokI GGATG 2 cut(s) 167, 539
Fsp4HI GCNGC 6 cut(s) 26, 273, 315, 353, 356, 394
GluI GCNGC 6 cut(s) 26, 273, 315, 353, 356, 394
GsaI CCCAGC 2 cut(s) 207, 729
HaeIII GGCC 2 cut(s) 51, 208
HapII CCGG 2 cut(s) 518, 711
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 272, 482
HincII GTYRAC 1 cut(s) 255
HindII GTYRAC 1 cut(s) 255
HinfI GANTC 3 cut(s) 16, 530, 540
HpaII CCGG 2 cut(s) 518, 711
Hpy166II GTNNAC 2 cut(s) 255, 408
Hpy188I TCNGA 1 cut(s) 41
Hpy188III TCNNGA 6 cut(s) 59, 97, 224, 479, 500, 589
Hpy8I GTNNAC 2 cut(s) 255, 408
Hpy99I CGWCG 3 cut(s) 10, 25, 50
HpyAV CCTTC 3 cut(s) 277, 620, 661
HpyCH4III ACNGT 2 cut(s) 177, 514
HpyCH4V TGCA 2 cut(s) 28, 385
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 62, 323
HpyF3I CTNAG 3 cut(s) 33, 402, 738
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 272, 482
Kzo9I GATC 3 cut(s) 36, 458, 521
LmnI GCTCC 2 cut(s) 511, 677
Lsp1109I GCAGC 6 cut(s) 12, 259, 326, 364, 367, 405
LweI GCATC 2 cut(s) 493, 724
MaeIII GTNAC 2 cut(s) 541, 586
MalI GATC 3 cut(s) 38, 460, 523
MboI GATC 3 cut(s) 36, 458, 521
MboII GAAGA 8 cut(s) 100, 143, 250, 299, 377, 642, 652, 672
MhlI GDGCHC 2 cut(s) 600, 687
MluCI AATT 3 cut(s) 10, 113, 264
MlyI GAGTC 1 cut(s) 549
MnlI CCTC 9 cut(s) 55, 228, 331, 412, 613, 626, 656, 711, 714
MroXI GAANNNNTTC 2 cut(s) 108, 580
MseI TTAA 3 cut(s) 116, 371, 425
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 2 cut(s) 518, 711
MspR9I CCNGG 3 cut(s) 519, 573, 711
MvaI CCWGG 1 cut(s) 573
MvnI CGCG 1 cut(s) 60
MwoI GCNNNNNNNGC 3 cut(s) 57, 62, 323
NciI CCSGG 2 cut(s) 519, 711
NdeII GATC 3 cut(s) 36, 458, 521
NlaIII CATG 2 cut(s) 272, 482
NlaIV GGNNCC 4 cut(s) 43, 498, 612, 699
NmuCI GTSAC 2 cut(s) 541, 586
NruI TCGCGA 1 cut(s) 60
NspV TTCGAA 1 cut(s) 14
PagI TCATGA 1 cut(s) 478
PdmI GAANNNNTTC 2 cut(s) 108, 580
PfeI GAWTC 2 cut(s) 16, 530
PfoI TCCNGGA 2 cut(s) 517, 571
PkrI GCNGC 6 cut(s) 27, 274, 316, 354, 357, 395
PleI GAGTC 1 cut(s) 548
PpsI GAGTC 1 cut(s) 548
PpuMI RGGWCCY 1 cut(s) 698
Psp5II RGGWCCY 1 cut(s) 698
Psp6I CCWGG 1 cut(s) 571
PspFI CCCAGC 2 cut(s) 203, 725
PspGI CCWGG 1 cut(s) 571
PspN4I GGNNCC 4 cut(s) 43, 498, 612, 699
PspPI GGNCC 4 cut(s) 41, 206, 497, 698
PspPPI RGGWCCY 1 cut(s) 698
PstI CTGCAG 1 cut(s) 30
PstNI CAGNNNCTG 2 cut(s) 470, 725
PvuII CAGCTG 1 cut(s) 203
RruI TCGCGA 1 cut(s) 60
SaqAI TTAA 3 cut(s) 116, 371, 425
SatI GCNGC 6 cut(s) 26, 273, 315, 353, 356, 394
Sau3AI GATC 3 cut(s) 36, 458, 521
Sau96I GGNCC 4 cut(s) 41, 206, 497, 698
SchI GAGTC 1 cut(s) 549
ScrFI CCNGG 3 cut(s) 519, 573, 711
SduI GDGCHC 2 cut(s) 600, 687
SfaNI GCATC 2 cut(s) 493, 724
SfcI CTRYAG 2 cut(s) 26, 510
SfuI TTCGAA 1 cut(s) 14
SinI GGWCC 3 cut(s) 41, 497, 698
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
Sse9I AATT 3 cut(s) 10, 113, 264
SsiI CCGC 1 cut(s) 707
StyD4I CCNGG 3 cut(s) 517, 571, 709
StyI CCWWGG 1 cut(s) 687
TaaI ACNGT 2 cut(s) 177, 514
TaqI TCGA 5 cut(s) 8, 14, 96, 159, 732
TaqII GACCGA 1 cut(s) 574
TasI AATT 3 cut(s) 10, 113, 264
TfiI GAWTC 2 cut(s) 16, 530
Tru1I TTAA 3 cut(s) 116, 371, 425
Tru9I TTAA 3 cut(s) 116, 371, 425
TseFI GTSAC 2 cut(s) 541, 586
TseI GCWGC 6 cut(s) 25, 272, 314, 352, 355, 393
Tsp45I GTSAC 2 cut(s) 541, 586
TspDTI ATGAA 3 cut(s) 206, 257, 461
TspGWI ACGGA 4 cut(s) 9, 98, 272, 672
VpaK11BI GGWCC 3 cut(s) 41, 497, 698
XapI RAATTY 2 cut(s) 10, 264
XmnI GAANNNNTTC 2 cut(s) 108, 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.