MD01G1193100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014009
Physical Location & Seq
Forward (+)
29193324 .. 29196684
3361 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1193100.v1.1.491

Sequence Viewer

Length: 747 bp
ATGGCGTCGAATTCGAATTTGAATCCGTCGCTGCAGCCTGAGATCGGACCCGACGGCCTTGCTCGCGAGGCTTCTGTCATCACCTACACTGAGAAGATAATCGAGAACGAACAGCTTCAATTAAGAAGATATATTGAAGAAAACTATTCTAAAATTCGTGATGTCGAACGAGAGCTGGCACAGTTGTCAATGGAGATGAAACTCACAGCTGGGCCAAAAAAATCAGCTCTTGAACATTTGAGGAAGAAAATAGAGATGTCAACGGAGAAAATTCATGCCGCCAAGCTGAAGGAAGAGCAGGCACGAAAGGTGTGGGAAGCAGCATTACAGGCTGTAAAGGACGAGGAAGCTATCAAGCAGCAGCTATGTGAAGACTTAAACCAACTGGTGCAAGAAAGCAGCAGTTCTCAGTTTAATAGACTGGAGGAGTTAAAAAGACGACTTGAAGCTATGAACCCAAGCAGATCATCAGCTTCCGTTTCTCATGATGGGAAATCAATGGGTCCAGATGCTCCTACAGTTCCGGGATCAAAAGAATCAGGTGGAGTCGCTCAAAACATCCCTAATCCAGGAAATGGTGGAAATGTTTCGGTCGCGAACGGGCACAATCAACAGCCTCCGGTTGAGGGAGAAGGAAGAGGAAAGAAGAAAAGTCAGTTCCACGGAAGAGGGAAGGGACTTGGAGCTGTGCCCAAGGGTAGAGGACCTCCTCCGCCGGGATGGACAGGGGCAGGGTTTGATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transcrip_act

Protein Analysis

249

Amino Acids

26.96

Weight (kDa)

8.53

Isoelectric Point (pI)

52.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transcrip_act PF04949 25 - 149 1.6e-09 Transcriptional activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014009)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51840
fragaria_vesca FvH4_7g28720 FvH4_7g28720
malus_domestica MD00G1004200.v1.1 MD01G1193100.v1.1
prunus_persica Prupe.2G287200_v2.0.a1
pyrus_communis pycom01g20370
rosa_chinensis RchiOBHm_Chr1g0376221
rosa_laevigata RLG00000026618
rosa_roxburghii Rroxscaffold_4G00282290
rosa_rugosa Rorug01G0394500 Rorug01G0394600
rosa_samantha Rh1AG406100 Rh1BG369800 Rh1CG382700 Rh1DG399600
rosa_wichuraiana Rw1G036030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 575
AccII CGCG 2 cut(s) 66, 596
AciI CCGC 2 cut(s) 279, 713
AclWI GGATC 1 cut(s) 535
AcsI RAATTY 4 cut(s) 10, 16, 153, 270
AcuI CTGAAG 1 cut(s) 308
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 5 cut(s) 44, 569, 575, 626, 716
AgsI TTSAA 5 cut(s) 22, 119, 137, 233, 446
AjnI CCWGG 1 cut(s) 568
AlwI GGATC 1 cut(s) 535
AoxI GGCC 2 cut(s) 55, 212
ApeKI GCWGC 6 cut(s) 31, 34, 320, 358, 361, 399
ApoI RAATTY 4 cut(s) 10, 16, 153, 270
Asp700I GAANNNNTTC 2 cut(s) 114, 586
AspS9I GGNCC 4 cut(s) 47, 212, 503, 704
AsuC2I CCSGG 2 cut(s) 525, 717
AsuHPI GGTGA 1 cut(s) 73
AsuII TTCGAA 1 cut(s) 14
AvaII GGWCC 3 cut(s) 47, 503, 704
BaeGI GKGCMC 2 cut(s) 606, 693
BarI GAAGNNNNNNTAC 2 cut(s) 309, 341
BbsI GAAGAC 1 cut(s) 378
BbvI GCAGC 6 cut(s) 18, 46, 332, 370, 373, 411
BccI CCATC 2 cut(s) 482, 714
BceAI ACGGC 1 cut(s) 70
BcgI CGANNNNNNTGC 2 cut(s) 41, 75
BciT130I CCWGG 1 cut(s) 570
BcnI CCSGG 2 cut(s) 525, 717
BfmI CTRYAG 2 cut(s) 32, 516
BisI GCNGC 7 cut(s) 32, 35, 279, 321, 359, 362, 400
BlsI GCNGC 7 cut(s) 33, 36, 280, 322, 360, 363, 401
Bme1390I CCNGG 3 cut(s) 525, 570, 717
Bme18I GGWCC 3 cut(s) 47, 503, 704
BmgT120I GGNCC 4 cut(s) 47, 212, 503, 704
BmiI GGNNCC 2 cut(s) 49, 504
BmrFI CCNGG 3 cut(s) 525, 570, 717
BmsI GCATC 2 cut(s) 499, 730
BpiI GAAGAC 1 cut(s) 378
BpmI CTGGAG 1 cut(s) 443
Bpu14I TTCGAA 1 cut(s) 14
BpuMI CCSGG 2 cut(s) 525, 717
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 661, 693
BsaWI WCCGGW 1 cut(s) 619
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 569, 575, 626, 716
Bse1I ACTGG 2 cut(s) 390, 426
BseBI CCWGG 1 cut(s) 570
BseDI CCNNGG 2 cut(s) 661, 693
BseGI GGATG 2 cut(s) 558, 725
BseLI CCNNNNNNNGG 5 cut(s) 44, 569, 575, 626, 716
BseMII CTCAG 3 cut(s) 30, 81, 422
BseNI ACTGG 2 cut(s) 390, 426
BseRI GAGGAG 2 cut(s) 440, 699
BseSI GKGCMC 2 cut(s) 606, 693
BseXI GCAGC 6 cut(s) 18, 46, 332, 370, 373, 411
BseYI CCCAGC 1 cut(s) 209
Bsh1236I CGCG 2 cut(s) 66, 596
Bsh1285I CGRYCG 1 cut(s) 594
BshFI GGCC 2 cut(s) 57, 214
BsiEI CGRYCG 1 cut(s) 594
BsiSI CCGG 3 cut(s) 524, 620, 716
BslFI GGGAC 1 cut(s) 690
BslI CCNNNNNNNGG 5 cut(s) 44, 569, 575, 626, 716
BsmFI GGGAC 1 cut(s) 690
BsnI GGCC 2 cut(s) 57, 214
Bsp119I TTCGAA 1 cut(s) 14
Bsp1286I GDGCHC 2 cut(s) 606, 693
Bsp143I GATC 3 cut(s) 42, 464, 527
Bsp68I TCGCGA 2 cut(s) 66, 596
BspACI CCGC 2 cut(s) 279, 713
BspANI GGCC 2 cut(s) 57, 214
BspCNI CTCAG 3 cut(s) 31, 82, 421
BspFNI CGCG 2 cut(s) 66, 596
BspHI TCATGA 1 cut(s) 484
BspLI GGNNCC 2 cut(s) 49, 504
BspMAI CTGCAG 1 cut(s) 36
BspPI GGATC 1 cut(s) 535
BspQI GCTCTTC 1 cut(s) 288
BspT104I TTCGAA 1 cut(s) 14
BsrI ACTGG 2 cut(s) 390, 426
BssECI CCNNGG 2 cut(s) 661, 693
BssMI GATC 3 cut(s) 42, 464, 527
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 693
Bst2UI CCWGG 1 cut(s) 570
Bst4CI ACNGT 2 cut(s) 183, 520
Bst6I CTCTTC 3 cut(s) 288, 631, 661
BstACI GRCGYC 1 cut(s) 5
BstBI TTCGAA 1 cut(s) 14
BstC8I GCNNGC 3 cut(s) 64, 177, 300
BstDEI CTNAG 4 cut(s) 39, 90, 408, 744
BstDSI CCRYGG 1 cut(s) 661
BstENI CCTNNNNNAGG 1 cut(s) 567
BstF5I GGATG 2 cut(s) 558, 725
BstFNI CGCG 2 cut(s) 66, 596
BstKTI GATC 3 cut(s) 45, 467, 530
BstMBI GATC 3 cut(s) 42, 464, 527
BstMCI CGRYCG 1 cut(s) 594
BstMWI GCNNNNNNNGC 3 cut(s) 63, 68, 329
BstNI CCWGG 1 cut(s) 570
BstSCI CCNGG 3 cut(s) 523, 568, 715
BstSFI CTRYAG 2 cut(s) 32, 516
BstSLI GKGCMC 2 cut(s) 606, 693
BstUI CGCG 2 cut(s) 66, 596
BstV1I GCAGC 6 cut(s) 18, 46, 332, 370, 373, 411
BstV2I GAAGAC 1 cut(s) 378
BsuRI GGCC 2 cut(s) 57, 214
BtgI CCRYGG 1 cut(s) 661
BtsCI GGATG 2 cut(s) 558, 725
BtsIMutI CAGTG 1 cut(s) 87
BtuMI TCGCGA 2 cut(s) 66, 596
Cac8I GCNNGC 3 cut(s) 64, 177, 300
CciI TCATGA 1 cut(s) 484
Cfr13I GGNCC 4 cut(s) 47, 212, 503, 704
CviAII CATG 2 cut(s) 275, 485
DdeI CTNAG 4 cut(s) 39, 90, 408, 744
DpnI GATC 3 cut(s) 44, 466, 529
DpnII GATC 3 cut(s) 42, 464, 527
Eam1104I CTCTTC 3 cut(s) 288, 631, 661
EarI CTCTTC 3 cut(s) 288, 631, 661
EciI GGCGGA 1 cut(s) 702
Eco130I CCWWGG 1 cut(s) 693
Eco47I GGWCC 3 cut(s) 47, 503, 704
Eco57I CTGAAG 1 cut(s) 308
EcoNI CCTNNNNNAGG 1 cut(s) 567
EcoO109I RGGNCCY 1 cut(s) 704
EcoRI GAATTC 1 cut(s) 10
EcoRII CCWGG 1 cut(s) 568
EcoT14I CCWWGG 1 cut(s) 693
ErhI CCWWGG 1 cut(s) 693
FaeI CATG 2 cut(s) 278, 488
FaiI YATR 5 cut(s) 132, 276, 367, 452, 486
FaqI GGGAC 1 cut(s) 690
FatI CATG 2 cut(s) 274, 484
Fnu4HI GCNGC 7 cut(s) 32, 35, 279, 321, 359, 362, 400
FokI GGATG 2 cut(s) 545, 732
Fsp4HI GCNGC 7 cut(s) 32, 35, 279, 321, 359, 362, 400
GluI GCNGC 7 cut(s) 32, 35, 279, 321, 359, 362, 400
GsaI CCCAGC 1 cut(s) 213
GsuI CTGGAG 1 cut(s) 443
HaeIII GGCC 2 cut(s) 57, 214
HapII CCGG 3 cut(s) 524, 620, 716
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 278, 488
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HinfI GANTC 3 cut(s) 22, 536, 546
HpaII CCGG 3 cut(s) 524, 620, 716
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 1 cut(s) 261
Hpy188I TCNGA 1 cut(s) 47
Hpy188III TCNNGA 7 cut(s) 65, 103, 158, 230, 485, 506, 595
Hpy8I GTNNAC 1 cut(s) 261
Hpy99I CGWCG 3 cut(s) 10, 31, 56
HpyAV CCTTC 3 cut(s) 283, 626, 667
HpyCH4III ACNGT 2 cut(s) 183, 520
HpyCH4V TGCA 2 cut(s) 34, 391
HpyF10VI GCNNNNNNNGC 3 cut(s) 63, 68, 329
HpyF3I CTNAG 4 cut(s) 39, 90, 408, 744
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 278, 488
Kzo9I GATC 3 cut(s) 42, 464, 527
LguI GCTCTTC 1 cut(s) 288
LmnI GCTCC 2 cut(s) 517, 683
Lsp1109I GCAGC 6 cut(s) 18, 46, 332, 370, 373, 411
LweI GCATC 2 cut(s) 499, 730
MalI GATC 3 cut(s) 44, 466, 529
MboI GATC 3 cut(s) 42, 464, 527
MboII GAAGA 9 cut(s) 106, 138, 149, 256, 305, 383, 648, 658, 678
MhlI GDGCHC 2 cut(s) 606, 693
MluCI AATT 5 cut(s) 10, 16, 119, 153, 270
MlyI GAGTC 1 cut(s) 555
MroXI GAANNNNTTC 2 cut(s) 114, 586
MseI TTAA 4 cut(s) 122, 377, 414, 431
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 3 cut(s) 524, 620, 716
MspR9I CCNGG 3 cut(s) 525, 570, 717
MvaI CCWGG 1 cut(s) 570
MvnI CGCG 2 cut(s) 66, 596
MwoI GCNNNNNNNGC 3 cut(s) 63, 68, 329
NciI CCSGG 2 cut(s) 525, 717
NdeII GATC 3 cut(s) 42, 464, 527
NlaIII CATG 2 cut(s) 278, 488
NlaIV GGNNCC 2 cut(s) 49, 504
NruI TCGCGA 2 cut(s) 66, 596
NspV TTCGAA 1 cut(s) 14
PagI TCATGA 1 cut(s) 484
PciSI GCTCTTC 1 cut(s) 288
PdmI GAANNNNTTC 2 cut(s) 114, 586
PfeI GAWTC 2 cut(s) 22, 536
PflMI CCANNNNNTGG 1 cut(s) 575
PfoI TCCNGGA 2 cut(s) 523, 568
PkrI GCNGC 7 cut(s) 33, 36, 280, 322, 360, 363, 401
PleI GAGTC 1 cut(s) 554
PpsI GAGTC 1 cut(s) 554
PpuMI RGGWCCY 1 cut(s) 704
Psp5II RGGWCCY 1 cut(s) 704
Psp6I CCWGG 1 cut(s) 568
PspFI CCCAGC 1 cut(s) 209
PspGI CCWGG 1 cut(s) 568
PspN4I GGNNCC 2 cut(s) 49, 504
PspPI GGNCC 4 cut(s) 47, 212, 503, 704
PspPPI RGGWCCY 1 cut(s) 704
PstI CTGCAG 1 cut(s) 36
PvuII CAGCTG 1 cut(s) 209
RruI TCGCGA 2 cut(s) 66, 596
SapI GCTCTTC 1 cut(s) 288
SaqAI TTAA 4 cut(s) 122, 377, 414, 431
SatI GCNGC 7 cut(s) 32, 35, 279, 321, 359, 362, 400
Sau3AI GATC 3 cut(s) 42, 464, 527
Sau96I GGNCC 4 cut(s) 47, 212, 503, 704
SchI GAGTC 1 cut(s) 555
ScrFI CCNGG 3 cut(s) 525, 570, 717
SduI GDGCHC 2 cut(s) 606, 693
SfaNI GCATC 2 cut(s) 499, 730
SfcI CTRYAG 2 cut(s) 32, 516
SfuI TTCGAA 1 cut(s) 14
SinI GGWCC 3 cut(s) 47, 503, 704
Sse9I AATT 5 cut(s) 10, 16, 119, 153, 270
SsiI CCGC 2 cut(s) 279, 713
StyD4I CCNGG 3 cut(s) 523, 568, 715
StyI CCWWGG 1 cut(s) 693
TaaI ACNGT 2 cut(s) 183, 520
TaqI TCGA 4 cut(s) 8, 14, 102, 165
TaqII GACCGA 1 cut(s) 580
TasI AATT 5 cut(s) 10, 16, 119, 153, 270
TauI GCSGC 1 cut(s) 281
TfiI GAWTC 2 cut(s) 22, 536
Tru1I TTAA 4 cut(s) 122, 377, 414, 431
Tru9I TTAA 4 cut(s) 122, 377, 414, 431
TscAI CASTG 1 cut(s) 94
TseI GCWGC 6 cut(s) 31, 34, 320, 358, 361, 399
TspDTI ATGAA 3 cut(s) 212, 263, 467
TspGWI ACGGA 4 cut(s) 15, 278, 466, 678
TspRI CASTG 1 cut(s) 94
Van91I CCANNNNNTGG 1 cut(s) 575
VpaK11BI GGWCC 3 cut(s) 47, 503, 704
XagI CCTNNNNNAGG 1 cut(s) 567
XapI RAATTY 4 cut(s) 10, 16, 153, 270
XmnI GAANNNNTTC 2 cut(s) 114, 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.