MD00G1006100.v1.1

Sorbitol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0018083
Physical Location & Seq
Forward (+)
824079 .. 824539
461 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1006100.v1.1.491

Sequence Viewer

Length: 321 bp
ATGGGTAAGGGAGGCCAATCCAGCAATGGCGTGGTAAGAGAAGCCAAACCTGTTGAGCAGCAAAACATGGCTGCTTGGCTCGTTGATGTTAACACCATCAAGATCCTACCTTTCAAGCTCCCCATTATCGGACCCCATGATGTTCGAATTCGGATCAAGGCTGTCGGCATTTGTGGAAGCGATGTCCACTACCTCAAGACCATGAAATGTGCGGATTTTGAGGTGAAAGAGCCAATGGTAATCGGACACGAGTGTGCAGGAATCGTAGACAAAGTTGGGAGCGCGGTGAAGCATTTGGTACCTGGGACCGTGTGGCGGTAG

Protein Analysis

107

Amino Acids

11.52

Weight (kDa)

9.12

Isoelectric Point (pI)

22.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 44 - 103 1.4e-14 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018083)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g28900
malus_domestica MD00G1006100.v1.1 MD13G1239400.v1.1 MD17G1261300.v1.1
pyrus_communis pycom01g20510 pycom07g24110
rosa_multiflora Rmu_sc0016049.1_g000005
rosa_roxburghii Rroxscaffold_4G00282120
rosa_samantha Rh1AG407500 Rh1CG385000 Rh1DG402000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 298
AccB1I GGYRCC 1 cut(s) 298
AccI GTMKAC 1 cut(s) 267
AccII CGCG 1 cut(s) 284
AciI CCGC 3 cut(s) 212, 284, 316
AclWI GGATC 2 cut(s) 97, 161
AcsI RAATTY 1 cut(s) 147
AfaI GTAC 1 cut(s) 300
AfiI CCNNNNNNNGG 2 cut(s) 128, 315
AgsI TTSAA 1 cut(s) 115
AjnI CCWGG 1 cut(s) 301
AleI CACNNNNGTG 1 cut(s) 252
AluBI AGCT 1 cut(s) 118
AluI AGCT 1 cut(s) 118
AlwI GGATC 2 cut(s) 97, 161
AoxI GGCC 1 cut(s) 13
ApeKI GCWGC 2 cut(s) 58, 71
ApoI RAATTY 1 cut(s) 147
Asp718I GGTACC 1 cut(s) 298
AspLEI GCGC 1 cut(s) 284
AspS9I GGNCC 2 cut(s) 131, 306
AsuHPI GGTGA 2 cut(s) 235, 298
AsuII TTCGAA 1 cut(s) 145
AvaII GGWCC 2 cut(s) 131, 306
BanI GGYRCC 1 cut(s) 298
BauI CACGAG 1 cut(s) 248
BbvI GCAGC 2 cut(s) 58, 70
BccI CCATC 1 cut(s) 104
BciT130I CCWGG 1 cut(s) 303
BisI GCNGC 2 cut(s) 59, 72
BlsI GCNGC 2 cut(s) 60, 73
Bme1390I CCNGG 1 cut(s) 303
Bme18I GGWCC 2 cut(s) 131, 306
BmgT120I GGNCC 2 cut(s) 131, 306
BmiI GGNNCC 3 cut(s) 133, 300, 307
BmrFI CCNGG 1 cut(s) 303
Bpu14I TTCGAA 1 cut(s) 145
BpuEI CTTGAG 1 cut(s) 179
BsaJI CCNNGG 1 cut(s) 302
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 315
Bse3DI GCAATG 1 cut(s) 31
BseBI CCWGG 1 cut(s) 303
BseDI CCNNGG 1 cut(s) 302
BseLI CCNNNNNNNGG 2 cut(s) 128, 315
BseMI GCAATG 1 cut(s) 31
BseXI GCAGC 2 cut(s) 58, 70
BsgI GTGCAG 1 cut(s) 276
Bsh1236I CGCG 1 cut(s) 284
BshFI GGCC 1 cut(s) 15
BshNI GGYRCC 1 cut(s) 298
BslI CCNNNNNNNGG 2 cut(s) 128, 315
BsnI GGCC 1 cut(s) 15
Bsp119I TTCGAA 1 cut(s) 145
Bsp143I GATC 2 cut(s) 102, 153
BspACI CCGC 3 cut(s) 212, 284, 316
BspANI GGCC 1 cut(s) 15
BspFNI CGCG 1 cut(s) 284
BspLI GGNNCC 3 cut(s) 133, 300, 307
BspPI GGATC 2 cut(s) 97, 161
BspT104I TTCGAA 1 cut(s) 145
BspT107I GGYRCC 1 cut(s) 298
BsrDI GCAATG 1 cut(s) 31
BssECI CCNNGG 1 cut(s) 302
BssMI GATC 2 cut(s) 102, 153
BssSI CACGAG 1 cut(s) 248
Bst2BI CACGAG 1 cut(s) 248
Bst2UI CCWGG 1 cut(s) 303
Bst4CI ACNGT 1 cut(s) 310
BstBI TTCGAA 1 cut(s) 145
BstFNI CGCG 1 cut(s) 284
BstHHI GCGC 1 cut(s) 284
BstKTI GATC 2 cut(s) 105, 156
BstMBI GATC 2 cut(s) 102, 153
BstMWI GCNNNNNNNGC 1 cut(s) 21
BstNI CCWGG 1 cut(s) 303
BstSCI CCNGG 1 cut(s) 301
BstUI CGCG 1 cut(s) 284
BstV1I GCAGC 2 cut(s) 58, 70
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
BsuRI GGCC 1 cut(s) 15
BtgZI GCGATG 1 cut(s) 195
CfoI GCGC 1 cut(s) 284
Cfr13I GGNCC 2 cut(s) 131, 306
Csp6I GTAC 1 cut(s) 299
CviAII CATG 3 cut(s) 67, 137, 202
CviJI RGCY 7 cut(s) 15, 44, 71, 79, 118, 161, 232
CviKI_1 RGCY 7 cut(s) 15, 44, 71, 79, 118, 161, 232
CviQI GTAC 1 cut(s) 299
DpnI GATC 2 cut(s) 104, 155
DpnII GATC 2 cut(s) 102, 153
Eco47I GGWCC 2 cut(s) 131, 306
EcoRI GAATTC 1 cut(s) 147
EcoRII CCWGG 1 cut(s) 301
FaeI CATG 3 cut(s) 70, 140, 205
FaiI YATR 3 cut(s) 68, 138, 203
FatI CATG 3 cut(s) 66, 136, 201
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 2 cut(s) 59, 72
Fsp4HI GCNGC 2 cut(s) 59, 72
GlaI GCGC 1 cut(s) 283
GluI GCNGC 2 cut(s) 59, 72
HaeIII GGCC 1 cut(s) 15
HhaI GCGC 1 cut(s) 284
Hin1II CATG 3 cut(s) 70, 140, 205
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HinfI GANTC 1 cut(s) 261
HpaI GTTAAC 1 cut(s) 91
HphI GGTGA 2 cut(s) 235, 298
Hpy166II GTNNAC 3 cut(s) 91, 187, 268
Hpy188I TCNGA 3 cut(s) 131, 153, 245
Hpy188III TCNNGA 2 cut(s) 100, 196
Hpy8I GTNNAC 3 cut(s) 91, 187, 268
HpyCH4III ACNGT 1 cut(s) 310
HpyCH4V TGCA 1 cut(s) 257
HpyF10VI GCNNNNNNNGC 1 cut(s) 21
Hsp92II CATG 3 cut(s) 70, 140, 205
HspAI GCGC 1 cut(s) 282
KpnI GGTACC 1 cut(s) 302
KspAI GTTAAC 1 cut(s) 91
Kzo9I GATC 2 cut(s) 102, 153
LmnI GCTCC 2 cut(s) 123, 279
LpnPI CCDG 5 cut(s) 34, 63, 243, 288, 315
Lsp1109I GCAGC 2 cut(s) 58, 70
MalI GATC 2 cut(s) 104, 155
MboI GATC 2 cut(s) 102, 153
MflI RGATCY 1 cut(s) 102
MluCI AATT 1 cut(s) 147
MnlI CCTC 3 cut(s) 5, 203, 214
MseI TTAA 1 cut(s) 90
MslI CAYNNNNRTG 1 cut(s) 252
MspR9I CCNGG 1 cut(s) 303
MvaI CCWGG 1 cut(s) 303
MvnI CGCG 1 cut(s) 284
MwoI GCNNNNNNNGC 1 cut(s) 21
NdeII GATC 2 cut(s) 102, 153
NlaIII CATG 3 cut(s) 70, 140, 205
NlaIV GGNNCC 3 cut(s) 133, 300, 307
NspV TTCGAA 1 cut(s) 145
OliI CACNNNNGTG 1 cut(s) 252
PfeI GAWTC 1 cut(s) 261
PkrI GCNGC 2 cut(s) 60, 73
Psp6I CCWGG 1 cut(s) 301
PspGI CCWGG 1 cut(s) 301
PspN4I GGNNCC 3 cut(s) 133, 300, 307
PspPI GGNCC 2 cut(s) 131, 306
PsuI RGATCY 1 cut(s) 102
RsaI GTAC 1 cut(s) 300
RsaNI GTAC 1 cut(s) 299
RseI CAYNNNNRTG 1 cut(s) 252
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 2 cut(s) 59, 72
Sau3AI GATC 2 cut(s) 102, 153
Sau96I GGNCC 2 cut(s) 131, 306
ScrFI CCNGG 1 cut(s) 303
SetI ASST 6 cut(s) 52, 112, 120, 195, 225, 304
SfuI TTCGAA 1 cut(s) 145
SinI GGWCC 2 cut(s) 131, 306
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
Sse9I AATT 1 cut(s) 147
SsiI CCGC 3 cut(s) 212, 284, 316
StyD4I CCNGG 1 cut(s) 301
TaaI ACNGT 1 cut(s) 310
TaqI TCGA 1 cut(s) 145
TasI AATT 1 cut(s) 147
TfiI GAWTC 1 cut(s) 261
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TseI GCWGC 2 cut(s) 58, 71
TspDTI ATGAA 1 cut(s) 218
VpaK11BI GGWCC 2 cut(s) 131, 306
XapI RAATTY 1 cut(s) 147
XcmI CCANNNNNNNNNTGG 2 cut(s) 23, 28
XmiI GTMKAC 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.